Outcomes of the Cost Action Agriculturally Important Toxigenic Fungi and perspectives of the FPVI Integrated project GOOD-FOOD :

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1 utcomes of the Cost Action Agriculturally Important Toxigenic Fungi and perspectives of the FPVI Integrated project GD-FD : ANTNI LGRIEC (ISPA- CNR, ITALY) CH H H N H CH 3 Cl H CH 3 CH CCH 2 CHCH 2 CH CH 3 H H H CH 3 CH 3 H CCH CHCH CH 2 2 CH NH 2 CH 3 H CH 2 CH 3 H MYC-GLBE Launch Conference

2 Agriculturally important toxigenic fungi The objective of CST Action 835 has been to promote, co-ordinate and harmonize European institutions which intend co-operate on agriculturally important toxigenic fungi in sustainable and competitive agriculture in Europe. The coordination is organized by a management committee in accordance with the memorandum signed by 20 European countries and to no Cost countries The action was composed of five Working-Groups listed below: WG1: Biodiversity, Phylogeny and Taxonomy of Toxigenic Fungi WG2: WG3: WG4: WG5: Genetics and Diagnosis of Toxigenic Fungi Ecology and Pathogenicity of Toxigenic Fungi Chemical and Biological characterization of Toxic Metabolites Toxigenic Fungi in Sustainable Agriculture: Biopesticides

3 BJECTIVES DEVELPED BY THE ACTIN to gather European institutions which intend to co-operate on agriculturally important TF in sustainable and competitive agriculture in Europe to provide the necessary know-how for increasing the competitiveness of European research at a crucial moment for the development of new management techniques in agriculture to promote biotechnology in the prevention (in case of mycotoxins accumulation), production and application (in case of biological control) of agriculturally important TF in sustainable agriculture.

4 Main outcomes from the CST 835 Six scientific books overviewing various topics: epidemiology, biodivesity, molecular tools, genomics, and biochemical aspects of agriculturally important TF. Five EU RTD projects in FPV has been financed by efforts and co-operation of EU researchers and Institutions which joined the Cost Action 835 Cost 835 Workshops and various Joint Meeting for each WGs Scientists have worked closely with their counterparts in other countries Cost 835 has promoted co-operation and lowered the fences around national research projects

5 Published by EU Commission in 2001 include a collection of all available data on this topic from all European Countries. Special issue published by Kluwer in 2002 with the aim to gather some specialist reports and opinion pieces on the specific topic Mycotoxin in Plant Disease. The book is of particular value for interdisciplinary scientists and especially for mycologists, mycotoxicologists and plant pathologist. The Workshop Epidemiology and ecology of mycotoxigenic fungi held in East Malling UK September 2002 has lead to the publication by Kluwer in 2003 of a special issue Epidemiology of Mycotoxin Producing Fungi

6 A fourth book entitled Molecular diversity and PCR-detection of toxigenic Fusarium species and ochratoxigenic fungi has been edit in 2004 in European Journal of Plant Pathology. The contributes of the Final CST Workshop held in Martina Franca (TA) Italy ctober 2003 is published by KLUWER in a book entitled An verview on Toxigenic Fungi and Mycotoxins in Europe with the contribution of 18 European countries.

7 EU RTD projects in FPV approved under the efforts of Cost 835 CNTRL MYCTX FD QLK1-CT Hazard analysis control of food contamination: Prevention of Fusarium mycotoxins entering the human and animal food chain DETX-FUNGI QLK Early detection of toxigenic Fusarium species and ochratoxigenic fungi in plant products RAMFIC QLK Sustainability, product safety and quality in cereals: development of novel quantitative models for risk assessment for mycotoxigenic Fusarium species WINE-CHRA RISK QLK Risk assessment and integrated ochratoxin A (TA) management in grape and wine RAFBCA QLK1-CT Risk Assessment of Fungal Biological Control Agents

8 During this project our CST colleague Dr. Wenche Langseth of the Norwegian National Veterinary Institute, passed away in She was very active as CST delegate, and her constant enthusiasm and sincere friendship is still in our mind. We took the opportunity in this action to dedicate a fungal species to her memory.: Fusarium langsethiae M. Torp and H.I. Niremberg International Journal of Food Microbiology Copyright 2004 Elsevier B.V. The European Sporotrichiella project: a polyphasic approach to the biology of a new Fusarium species Edited by M. Torp, H. Nirenberg and U. Thrane Volume 95, Issue 3, Pages (15 September 2004)

9 Food Safety and Quality with Microsystems MYC-GLBE Launch Conference

10 What Microsystems can contribute to Agrofood? Current solution based on: Lab analysis (costly and time consuming) Expert human intervention (subjectivity) Destructive, not massive GoodFood Micro & Nano Systems alternative: Fast response and less reactive consumption Electric output low cost Small dimensions - portability Autonomous Functioning (Non human intervention, non- expert based systems) Pervasive and ubiquitous solutions Compliant with with the the Ambient Intelligent vision

11 Safety solutions Fast, multichannel and portable screening tools Biosensors, Inmunosensors DNA Chips µtas 1 FLW 2 3 UREA 4 MAGNET MAGNET

12 Logistics and Quality control solutions Multisensors e-nose e-tongue µfluidics Flexible substrates Autonomous power source Sensors NH 3,C 2 H 4 Unconditioned Sensor Temperature Humidity NH 3 C 2 H 4 Conditioning Electronics Microcontroller µp NH 3, C 2 H 4 Sensor Conditioning Module EEPRM Communication Interface + _ Power Source Communic ation Interface Module AmI Flexible Tag Substrate

13 Milk and Dairy Products Food Targets Fruits, juices and wine Fish WP1 WP2 WP3 WP4 Antibiotics Pesticides Mycotoxins Pathogens Antibiotics Pesticides Mycotoxins Pathogens WP5 Quality & Freshness WP6 Logistics & Storage Safety Quality Safety Quality WP7 Ambient Intelligence, Integration, Interconnection

14 Foodchain monitoring: safety and quality assessment stages Farmers Industrials Retailers Consumers Recollection Transport Processing Transport Storage (transforming, poisoning) (degrading) Consumption Storage (active-evolving, poisoning) Safety and Quality Assessment with M&NT MST contribution: closeness to the foodstuff & power of analysis & speed (multisensing, multipoint sensing, continuous monitoring, automation/non-specialist intervention)

15 Ambient Intelligence: Solutions for the interconnection MYC-GLBE Launch Conference PRDUCE PRDUCE SENSE SENSE PRCESS PRCESS & DECIDE DECIDE ACT ACT Vineyard: AmI for GoodFood Pilot Site Distributed network of sensors, for spatialtemporal monitoring of the environment process AmI process & DSS: for storing safety, quality and traceability related information; End-users, food quality specialists, nutritionists and consumers associations interact with the AmI platform

16 GoodFood Metrics Budget: Nº Partners: M Euros Nº countries: 10 Personpower 2071 Starting date: Duration: months

17 GoodFood Consortium Companies Research Centers Universities ACTEL (E) AZTI (E) CRANFIELD U. (UK) APIBI (F) CEA (F) SSENCE-LiU (S) BINSTRA (E) CNR (I) SSSA (I) BISENSE (B) CSIC (E) MIDRA (I) CSEM (CH) EPFL (CH) U. Barcelona (E) EADS (G) IPM-FhG (G) U. Pol. Catalunya (E) MICRZNE (UK) HTA (CH) U. Rovira i Virgili (E) NESTLE (CH) IMEL (Gr) U. Valladolid (E) SYNAPSIS (I) TENCAS DE CASASECA (E) IRST (I) NMRC (Ie) TEKNIKER (E) Users Club

18 WP3: Mycotoxins & Toxigenic Fungi bjectives of the WP3 Development of rapid, robust and user friendly diagnostic sensors for toxigenic fungi (DNA arrays) and mycotoxins (Multi-analyte immunosensosrs- nanoelectrode arrays) Toxigenic Fungi: Aspergillus section nigri in grape & Penicillium expansum in apples Mycotoxins: aflatoxin M1 in milk ; patulin in fruit juices; ochratoxin A in must & wine

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