BIOZYME is a product in use by Latin American farmers in a wide range of crops to provide outstanding results for more than 18 years.
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1 BIOZYME BIOZYME TM is a unique formulation developed to enhance plant and crop performance. It influences plant nutrient uptake that enhances fruit set, fruit numbers, fruit quality and general crop performance. BIOZYME was developed for the demanding high yielding crop system that requires better quality and higher yield. INTRODUCTION BIOZYME is a bio-stimulant that combines the power of natural plant extracts and naturally occurring macro and micronutrients to enhance crop performance and physiological processes in crops and plants. BIOZYME is a product in use by Latin American farmers in a wide range of crops to provide outstanding results for more than 18 years. BIOZYME can provide better and more uniform fruit development under high crop demanding situations. BIOZYME enhances fruit quality parameters in vegetables and fruit crops such as total soluble solid content (Brix level), carbohydrate content, increases sugar and flavor, and increase antioxidant and vitamins levels. BIOZYME balanced-composition gives its unique and innovative properties that propel and stimulate plant growth, plant nutrition uptake, fruit development and consequently better yield performance. BIOZYME can be applied before and/or during flowering, fruit setting and fruit growth as a foliar spray. It is a safe-to-use product and is not phytotoxic. The application ensures required plant nutrients that optimize fruit setting and activates the growth of bigger and tender fruits.
2 WHAT IS BIOZYME MADE OF? COMPOSITION: Weight (% w/w): Natural plant extract 78.0 Macronutrient -Magnesium -Sulfur Micronutrient -Manganese -Iron -Zinc -Boron Inert materials 20.4 What do these components will provide to you? Natural origin extract from plants Favors the availability of provided nutrients Macro-Nutrient Content Magnesium (Mg ) Sulfur (S) Magnesium is part of the chlorophyll in all green plants and essential for photosynthesis Activate many plant enzymes needed for growth Activator of photosynthesis Is structural part of amino acids Essential plant food for production of protein. Promotes activity and development of enzymes and vitamins Helps in chlorophyll formation
3 Improves root growth and seed production Helps with vigorous plant growth and resistance to cold Micro-Nutrients Contents Manganese (Mn) Activator of photosynthesis and other metabolites Iron (Fe) Part of the chlorophyll molecule Involved in chlorophyll synthesis Involved in redox reactions in respiration and photosynthesis Zinc (Zn) Involved in metabolic reactions Availability enhanced significantly by presence of mycorrhizal fungi in the soil Boron (B) Activator of metabolic process Facilitates transport of sugars across cell membranes Increases calcium efficiency in the plant Involved with pollen viability and good seed set Prevents the excessive conversion of sugars into starch Increases elongation growth of primary and lateral roots BIOZYME SOME FACTS BIOIZYME has been investigated and results of research are available in over 50 peer-reviewed publications around the world BIOZYME can increase plant nutrient uptake BIOZYME can increase capacity to develop more and better fruits BIOZYME can increase quality of fruits BIOZYME can increase activity of chlorophyll and photosynthesis BIOZYME can increase crop yield
4 RELEVANT RESEARCH PEER REVIEW CITATIONS ARE: BIOZYME applied as a foliar application enhanced zinc uptake much higher than typical mineral zinc application alone in soybean plants even in soils with acceptable levels of available zinc in Brazil. Zinc fertilization increased soybean yield, even in a soil with micronutrient availability above critical level. (Ferreira, L., et al, 2012). BIOZYME applied to citrus trees Washington Navel and Thomson (Citrus sinensis (L) Osb.) increased fruit set and final fruits retained per plant (Galvan et al, 2010). The use of BIOZYME complemented the mineral fertilization in lettuce production increasing commercial yields (Garcia, A., and J. Leon, 2010). In Ghana, Africa, BIOZYME as a foliar application increased total fruit yields in treated plants, slightly increased the chlorophyll content of the leaves, and increased seed number per fruit seemingly played a significant role in enhancing the yield of tomato, and may be applied at a rate of 500 ml ha-1 for maximum effect (J. Ofosu-Anim, E. T. Blay & L. Bening, 2007). Plants treated with BIOZYME resulted in higher fruit set, yield, higher fruit weight and volume, more total soluble solids, higher total sugars and longer shelf life (Sharma et al 2009). The application of BIOZYME to pepper plants (Capsicum annun) can increase plant nitrate assimilation and increase foliar dry weight and fruit yield per plant (Romero, L, 2002) BIOZYME can increase tuber number, sets and commercial seed when applied to potato seed production (Caldiz, D, 1996). Foliar application of BIOZYME to pepper (Capsicum annuum L.) grown under greenhouse induced higher levels and content of nonstructural carbohydrates, glucose, sucrose, and fructose (Belekbir et al, 1996). BIOZYME significantly improved emergence rate, percent emergence, final stand and number of ears of sweet corn in the first planting, and the percent emergence final stand, plant dry weight, and number of ears in the second planting (Campos-Cruz, et al 1994). BIOZYME can increase yield and weight of fruit in tomato Lycupersicum sculentum (Quintalan and Rojas 1990).
5 MODE OF ACTION BIOZYME has a unique composition that combines essential nutrients with natural plant extracts that biologically activate plant functions. The activation of plant functions help the formation of plant metabolites and trigger biochemical reactions that promotes plant growth and performance. Demonstration of responses of BIOZYME impact in tomato roots at different doses. Research studies have demonstrated that application of BIOZYME stimulates natural plant production of compounds that enhances cell division and elongation. In bioassay tests performed at the University of Sao Paulo Brazil, research results indicated that BIOZYME provided consistently physiological activation that typically favors plant growth. Results indicated responses after 5 days. Treatments were water, 0.5, 1, 2 and 4 ml/l-water of BIOZYME (Source: University of Sao Paulo Brazil).
6 Impact of BIOZYME in tomato roots also treated with water (control) and 0.5, 1, 2 and 4 ml/l-water of BIOZYME (Source: University of Sao Paulo Brazil) BIOZYME not only provided required nutrients for cell activation but also stimulate cellular differentiation, ensuring the number and strength of floral buds that contribute to a higher number of fruits. The application of BIOZYME during fruit growth results in the formation of more epidermis cells, allowing the fruits to increase their size and commercial grade consistency. BIOZYME affects positively some important physiological processes such as photosynthesis, nutrient and carbohydrates translocation-intensively to growing
7 fruits. As a result of these positive metabolic changes, the yields increased and quality and uniformity of fruits are improved. BIOZYME RECOMMENDATION: Crops Rate (L/ha) Application Time Pepper, Tomato, Eggplant st application before first flowering stage, then repeat every 2-3 weeks Cucumber, Melon, Watermelon st application before first flowering stage, then repeat every 2 weeks Strawberry st application before first flowering stage, then repeat every 4 weeks Pomegranate st application at green-pink (white) bud stage, then repeat every 2-3 weeks until natural fruit drop Citrus Fruits st application before first flowering stage, then repeat every 2-3 weeks Grapes st application at beginning of flowering, 2nd application just after fruit setting, 3rd application at pea size berries Rice st application at beginning of stem elongation, 2nd application at beginning of flowering Mustard st application at stem elongation, 2nd application at beginning of flowering Beans st application from budding stage to beginning of flowering, repeat after 2-3 weeks Wheat st application at the end of tillering, 2nd application at late flag leaf stage Rose st application before first flowering stage, then repeat every 2-3 weeks
8 STABILITY BIOZYME is a stable product and remains without changes for up to 24 months. BIOZYME requires storage under regular conditions of humidity and temperature and under such circumstances the properties of its effectiveness is kept for up to 24 months. COMPATIBILITY BIOZYME is compatible with most insecticides, fungicides, herbicides and fertilizers of agricultural use. BIOZYME does not produce plant toxicity when used at the recommended rates. DIRECTIONS FOR USE BIOZYME should be applied as a foliar spray, mixed with enough water to ensure proper plant coverage. BIOZYME can be applied using any type of spraying equipment. ADVANTAGES OF USING BIOZYME Increases the capacity of the plant to uptake essential nutrients resulting in increasing number of flower buds and improves flower setting, total number of fruits per plant enhances the size, uniformity and quality of fruits. STORAGE AND TRANSPORT CONDITIONS Store it in cool, dry places. Keep in its original packaging and closed. Keep away from food, drink, animal feeding and medicine.
9 RESULTS OF BIOZYME IN TOMATO Figure 1. Evaluation of BiIOZYME rates (1.0, 1.5 and 2.0 L/ha, UTC = Untreated control) on tomato, (Lycopersicum sculentum) variety Rio Grande determinate growth, fruit set. Trials conducted in Compostela, State of Nayary, Mexico, BIOZYME at 2 L/ha shows an increment of 47% of number of fruit set per cluster, also increase 17.8% fruit weight (data not shown), followed by BIOZYME 1.5 and 1 L/ha with increase of 28 and 23.5%, last one also increased the fruit weight by 12.6% (data not shown).
10 Figure 2. Evaluation of rates of BIOZYME (1.0, 1.5 and 2.0 L/ha, UTC = Untreated control) on tomato, (Lycopersicum sculentum) var. Rio Grande determinate growth, yield performance at different cuts. Trials conducted in Compostela, State of Nayary, Mexico, BIOZYME 1 L/ha, 89% more yield than control, followed by BIOZYME 1.5 and 2 L/ha with 53% more than UTC, however, there were no statistical difference between BIOZYME treatments (total harvest), all treatments were different with the control. Despite BIOZYME 2 L/ha increase the weight of fruits, BIOZYME 1 L/ha increase weight of fruits as well the number of fruit set.
11 Figure 3. Evaluation of rates of BIOZYME (0.5L/ha 3x, 1L/ha 3x, 1.5L/ha 3x, 2L/ha 3x, 1L/ha, UTC=untreated) on percentage of tomato (Lycopersicum sculentum) fruit set. Trials conducted in Quitman, State of Georgia, USA in All treatments with BIOZYME increased fruit set. BIOZYME 1 and 1.5 L/ha 3x had an increment of 28 and 34.5% of fruits per cluster, respectively; also BIOZYME 1 L/ha 3x increase weight of XL (15%), and LG (3%) fruits, as well as the number of XL fruits, all values compared with the control.
12 SUMMARY Biozyme: Is a unique formulated product that combines nutrients with natural plant extract to provide better crop plant nutrient uptake and performance at fruiting stage. Is designed for increasing plant performance including yield and fruit quality parameters. Only contains natural extracts combined with a set of essential nutrients that provides suitable performance for crops
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