Regulating the Internal Environment. AP Biology

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1 Regulating the Internal Environment

2 Conformers vs. Regulators Two evolutionary paths for organisms regulate internal environment maintain relatively constant internal conditions conform to external environment allow internal conditions to fluctuate along with external changes osmoregulation thermoregulation regulator regulator conformer conformer

3 Homeostasis Keeping the balance animal body needs to coordinate many systems all at once temperature blood sugar levels energy production water balance & intracellular waste disposal nutrients ion balance cell growth maintaining a steady state condition

4 Regulating the Internal Environment Water Balance & Nitrogenous Waste Removal

5 Animal systems evolved to support multicellular life CH CHO aa O 2 O 2 aa CO 2 NH 3 aa CH CHO O 2 aa CO 2 NH 3 CO 2 NH 3 CO 2 intracellular waste CO 2 NH 3 O 2 CO 2 O 2 aa NH 3 NH 3 CO 2 CO 2 NH 3 NH 3 CO 2 CO 2 CO 2 NH 3 CHO CH aa Diffusion too slow! extracellular waste

6 Overcoming limitations of diffusion Evolution of exchange systems for distributing nutrients circulatory system removing wastes excretory system aa CO CO 2 2 NH NH 3 3 CO 2 NH 3 CO 2 CO 2 O 2 systems to support multicellular organisms O 2 aa CH NH 3 NH 3 CO 2 CO 2 NH 3 NH 3 CO 2 CO 2 CO 2 NH 3 aa CHO

7 Osmoregulation Water balance freshwater hypotonic water flow into cells & salt loss saltwater hypertonic water loss from cells land dry environment need to conserve water may also need to conserve salt hypotonic hypertonic Why do all land animals have to conserve water? always lose water (breathing & waste) AP may Biology lose life while searching for water

8 Intracellular Waste What waste products? Animals poison themselves from the inside by digesting proteins! what do we digest our food into carbohydrates = CHO CO 2 + H 2 O lipids = CHO CO 2 + H 2 O proteins = CHON CO 2 + H 2 O + N lots! nucleic acids = CHOPN CO 2 + H 2 O + P + N very little cellular digestion cellular waste NH 2 = ammonia H H H O N C C OH R CO 2 + H 2 O

9 Nitrogenous waste disposal Ammonia (NH 3 ) very toxic carcinogenic very soluble easily crosses membranes must dilute it & get rid of it fast! How you get rid of nitrogenous wastes depends on who you are (evolutionary relationship) where you live (habitat) aquatic terrestrial terrestrial egg layer

10 Nitrogen waste Aquatic organisms can afford to lose water ammonia most toxic Terrestrial need to conserve water urea less toxic Terrestrial egg layers need to conserve water need to protect embryo in egg uric acid least toxic

11 Freshwater animals Water removal & nitrogen waste disposal remove surplus water use surplus water to dilute ammonia & excrete it need to excrete a lot of water so dilute ammonia & excrete it as very dilute urine also diffuse ammonia continuously through gills or through any moist membrane overcome loss of salts reabsorb in kidneys or active transport across gills

12 Land animals Nitrogen waste disposal on land need to conserve water must process ammonia so less toxic urea = larger molecule = less soluble = less toxic 2NH 2 + CO 2 = urea produced in liver kidney filter solutes out of blood reabsorb H 2 O (+ any useful solutes) excrete waste urine = urea, salts, excess sugar & H 2 O urine is very concentrated H N concentrated NH 3 would be too toxic mammals H H N H Urea costs energy to synthesize, but it s worth it! C O

13 Egg-laying land animals Nitrogen waste disposal in egg no place to get rid of waste in egg need even less soluble molecule uric acid = BIGGER = less soluble = less toxic birds, reptiles, insects itty bitty living space!

14 H O N Uric acid N H Polymerized urea large molecule O H N N H O And that folks, is why most male birds don t have a penis! precipitates out of solution doesn t harm embryo in egg white dust in egg adults still excrete N waste as white paste no liquid waste uric acid = white bird poop!

15 Mammalian System Filter solutes out of blood & reabsorb H 2 O + desirable solutes Key functions filtration fluids (water & solutes) filtered out of blood reabsorption selectively reabsorb (diffusion) needed water + solutes back to blood secretion pump out any other unwanted solutes to urine excretion expel concentrated urine (N waste + solutes + toxins) from body blood filtrate concentrated urine

16 Mammalian Kidney inferior vena cava aorta adrenal gland kidney ureter bladder urethra renal vein & artery nephron epithelial cells

17 Nephron Functional units of kidney 1 million nephrons per kidney Function filter out urea & other solutes (salt, sugar ) blood plasma filtered into nephron high pressure flow selective reabsorption of valuable solutes & H 2 O back into bloodstream greater flexibility & control why selective reabsorption & not selective filtration? counter current exchange system

18 Mammalian kidney Interaction of circulatory & excretory systems Circulatory system glomerulus = ball of capillaries Excretory system nephron Bowman s capsule loop of Henle proximal tubule descending limb ascending limb distal tubule collecting duct Glomerulus Bowman s capsule How can different sections allow the diffusion of different molecules? Glucose Amino acids Mg ++ Ca ++ Proximal tubule H 2 O H 2 O H 2 O Loop of Henle Na + Cl - Na + Cl - Distal tubule H 2 O H 2 O Collecting duct H 2 O

19 Nephron: Filtration At glomerulus filtered out of blood H 2 O glucose salts / ions urea not filtered out cells proteins high blood pressure in kidneys force to push (filter) H 2 O & solutes out of blood vessel BIG problems when you start out with high blood pressure in system hypertension = kidney damage

20 Nephron: Re-absorption Proximal tubule reabsorbed back into blood NaCl active transport of Na + Cl follows by diffusion H 2 O glucose HCO 3 - bicarbonate buffer for blood ph Descending limb Ascending limb

21 Nephron: Re-absorption Loop of Henle descending limb high permeability to H 2 O many aquaporins in cell membranes low permeability to salt few Na + or Cl channels reabsorbed H 2 O Descending limb structure fits function! Ascending limb

22 Nephron: Re-absorption Loop of Henle ascending limb structure fits function! low permeability to H 2 O Cl - pump Na + follows by diffusion different membrane proteins reabsorbed Descending limb Ascending limb salts maintains osmotic gradient

23 Nephron: Re-absorption Distal tubule reabsorbed salts H 2 O HCO - 3 bicarbonate

24 Nephron: Reabsorption & Excretion Collecting duct reabsorbed H 2 O excretion concentrated urine passed to bladder impermeable lining Descending limb Ascending limb

25 Osmotic control in nephron How is all this re-absorption achieved? tight osmotic control to reduce the energy cost of excretion use diffusion instead of active transport wherever possible the value of a counter current exchange system

26 Summary Not filtered out cells proteins remain in blood (too big) Reabsorbed: active transport Na + amino acids Cl glucose Reabsorbed: diffusion Na + Cl H 2 O Excreted urea why selective reabsorption & not selective filtration? excess H 2 O excess solutes (glucose, salts) toxins, drugs, unknowns

27 Any Questions?

28 Regulating the Internal Environment Maintaining Homeostasis

29 Negative Feedback Loop hormone or nerve signal gland or nervous system lowers body condition (return to set point) high sensor specific body condition sensor low raises body condition (return to set point) gland or nervous system hormone or nerve signal

30 Nervous System Control Controlling Body Temperature nerve signals brain sweat high body temperature low dilates surface blood vessels constricts surface blood vessels shiver brain nerve signals

31 Endocrine System Control Blood Osmolarity ADH pituitary increased water reabsorption increase thirst high blood osmolarity blood pressure low nephron ADH = AntiDiuretic Hormone

32 Maintaining Water Balance Get more water into blood fast High blood osmolarity level too many solutes in blood dehydration, high salt diet stimulates thirst = drink more release ADH from pituitary gland antidiuretic hormone increases permeability of collecting duct & reabsorption of water in kidneys increase water absorption back into blood decrease urination Alcohol suppresses ADH makes you urinate a lot! H 2 O H 2 O H 2 O

33 Endocrine System Control Blood Osmolarity Oooooh, zymogen! adrenal gland high increased water & salt reabsorption in kidney aldosterone blood osmolarity blood pressure angiotensin low nephron renin JGA = JuxtaGlomerular Apparatus JGA angiotensinogen

34 Maintaining Water Balance Low blood osmolarity level or low blood pressure JGA releases renin in kidney renin converts angiotensinogen to angiotensin angiotensin causes arterioles to constrict increase blood pressure angiotensin triggers release of aldosterone from adrenal gland increases reabsorption of NaCl & H 2 O in kidneys puts more water & salts back in blood Why such a rapid response system? Spring a leak? Get more water & salt into blood fast! adrenal gland

35 Endocrine System Control Blood Osmolarity ADH pituitary increased water reabsorption increase thirst adrenal gland high increased water & salt reabsorption aldosterone blood osmolarity blood pressure angiotensin low nephron renin nephron JuxtaGlomerular Apparatus angiotensinogen

36 Don t get batty Ask Questions!!

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