PARASITOLOGY INTRODUCTION
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1 PARASITOLOGY INTRODUCTION
2 DEFINITION Parasite means : one who eats at the table of another. Differs from bacteria and viruses in their complex life cycles, intermediate hosts and chronicity. Evolved to live under totally different environments according to the developmental stage and different host tissues.
3 SIGNIFICANCE Parasitic infections cause a lot of morbidity worldwide : 2nd most common infectious cause of blindness. One billion people harbour intestinal nematodes. Schistosomiasisaffects million people. Filariaeaffect a similar number as well. Several hundred million people contract malaria every year, with the death of 1 million children.
4 DEFINITIONS PARASITE PRIMARY HOST : NORMALLY HUMAN, MAY BE AN ANIMAL. INTERMEDIATE HOST : NORMALLY ANIMAL OR INSECT, HUMANS (ACCIDENTAL). NOT ALWAYS REQUIRED.
5 DEFINITIONS VECTOR. DEAD END HOST. OCCASIONALLY SAME ANIMAL MAY SERVE BOTH AS PRIMARY AND INTERMEDIATE HOST. RESERVOIR. ZOONOSIS.
6 DEFINITIONS PARASITES MAY BE PRESENT IN THE LUMEN OF AN ORGAN ( LUMINAL PARASITES ). PARASITES INFECTING TISSUES ARE CALLED TISSUE PARASITES. ENDOPARASITES. ECTOPARASITES.
7 LIFE CYCLE DIRECT SPREAD : PRIMARY HOST -TO- PRIMARY HOST THROUGH EGGS, CYSTS, PARASITE (RARE). SPREAD THROUGH AN INTERMEDIATE HOST: PRIMARY HOST - INTERMEDIATE HOST (MAY BE MORE THAN ONE) - PRIMARY HOST.
8 CLASSIFICATION TWO GROUPS OF PARASITES. 1)- PROTOZOA. 2)- HELMINTHS ( WORMS ).
9 PROTOZOA UNICELLULAR ORGANISMS. SIZE RANGE: 3 MICRONS MICRONS. CAN BE EITHER INTRACELLULAR OR EXTRACELLULAR PARASITES. VEGETATIVE FORM OF THE ORGANISM IS USUALLY REFERRED TO AS TROPHOZOITE.
10 INTRACELLULAR/EXTRACELLULAR
11 TRANSMISSION TROPHOZOITE IS USUALLY UNABLE TO SURVIVE OUTSIDE THE BODY LONG ENOUGH TO TRANSMIT INFECTION. TRANSMISSION IS THROUGH A TOUGH INERT FORM OF THE ORGANISM viz. A CYST. WHERE THERE IS AN INTERMEDIATE HOST NO CYST EXISTS, BUT ANOTHER FORM OF THE ORGANISM MAY BE INVOLVED.
12 CYST FORMATION
13 MULTIPLICATION PROTOZOA MULTIPLY THROUGH BINARY FISSION. THUS THE NUMBER OF ORGANISMS CAN INCREASE IN THE HOST. THAT CAN LEAD TO SERIOUS PATHOLOGY IN THE HOST.
14 MULTIPLICATION BINARY FISSION IS ASEXUAL REPRODUCTION. SOME PROTOZOA viz. SPOROZOA ALSO HAVE SEXUAL REPRODUCTION AT SOME STAGE OF THEIR LIFE CYCLE : MACROGAMETOCYTES MICROGAMETOCYTES ZYGOTE
15 MULTIPLICATION SEXUAL/ASEXUAL REPRODUCTION USUALLY OCCURS IN EITHER THE PRIMARY OR THE INTERMEDIATE HOST. IN CASE THERE IS ONLY ONE HOST BOTH CAN OCCUR IN THE SAME HOST. SEXUAL REPRODUCTION CAN ALSO BE ACCOMPANIED BY ASEXUAL MULTIPLICATION WITHIN THE HOST.
16 PLASMODIUM LIFE CYCLE
17 CLASSIFICATION PROTOZOA CAN BE CLASSIFIED ACCORDING TO LOCOMOTION ORGANELLES : RHIZOPODA (PSEUDOPODIA) FLAGELLATES. MEMBRANE. CILIATES ALSO UNDULATING SPOROZOA (APICOMPLEXA)
18 FLAGELLATES
19 CILIATE
20
21 HELMINTHS MUTICELLULAR ORGANISMS. SIZE RANGES FROM 2 mm TO 10 m. ALL ARE EXTRACELLUAR PATHOGENS. CAN BE TISSUE OR LUMINAL PARASITES. PRODUCE EGGS OR LARVAE.
22 CLASSIFICATION NEMATOHELMINTHS, NEMATODES OR ROUND WORMS. PLATYHELMINTHS : FLAT WORMS - CESTODES ( TAPEWORMS ). - TREMATODES ( FLUKES ).
23 NEMATODES CYLINDRICAL WORMS SIZE 2 mm. TO 1m. IN LENGTH. TISSUE OR LUMINAL PARASITES. LIFE SPAN WEEKS TO SEVERAL YEARS. SEPARATE SEXES. PRODUCE EGGS OR LARVAE.
24 MORPHOLOGY CUTICLE. GASTROINTESTINAL TRACT. EXCRETORY SYSTEM. NERVOUS SYTEM. MALE AND FEMALE REPRODUCTIVE SYSTEMS. LUMINAL NEMATODE POSSESS DIFFERENT MEANS FOR ATTACHMENT.
25 NEMATODE STRUCTURE
26 Scanning electron micrograph of the oral opening of Ancylostoma duodenale, another species of human hookworm. Note the presence of four cutting "teeth," two on each side.
27 LIFE CYCLE MANY ( LUMINAL ) DO NOT REQUIRE AN INTERMEDIATE HOST. DIRECT SPREAD BY MEANS OF EGGS. OTHERS ( TISSUE ) REQUIRE AN INTERMEDIATE HOST. PRODUCTION OF LARVAE. INTERMEDIATE HOST IS USUALLY AN ARTHROPOD.
28 HELMINTHS EGGS
29 MICROFILARIA
30 CESTODES FLATTENED. TAPE LIKE. SIZE 1 cm TO 10 m. CONSIST OF UNITS CALLED PRGLOTTIDS. EACH UNIT IS A SEPARATE ENTITY. NUMBER OF PROGLOTTIDS RANGES FROM 3 TO TISSUE OR LUMINAL PARASITE. PRODUCE EGGS.
31 CESTODES WITH ONE EXCEPTION ALL REQUIRE AN INTERMEDIATE HOST FOR TRANSMISSION. LIFE SPAN FROM WEEKS TO MANY YEARS. HERMAPHRODITE : WORM IS BOTH MALE AND FEMALE.
32 MORPHOLOGY SCOLEX : FOR ATTACHMENT. SUCKER : ROUND SHAPED USUALLY, MAY BE ELONGATED. HOOKS : SHAPED IN A RING CALLED ROSTELLUM, MAY BE SINGLE OR DOUBLE. NECK REGION FROM WHICH PROGLOTTIDS PROLIFERATE.
33 Cestode morphology
34 Cestode (Tapeworm) Body Structure: Scolex and Proglottids
35 Tapeworm Scolex
36 CESTODES EARLY PROGLOTTIDS ARE IMMATURE. FOLLOWED BY MATURE THAT EXHIBIT REPRODUCTIVE ORGANS : UTERUS, OVARIES, TESTES. GRAVID PROGLOTTIDS CONTAIN DILATED UTERUS WITH LATERAL BRANCHES FULL OF FERTILIZED EGGS.
37 Pork Tapeworm Uterine branches in proglottid
38 CESTODE
39 CESTODES THE SURFACE IS COVERED BY TEGUMENT WITH MICROTRICHIA TO INCREASE SURFACE AREA FOR ABSORPTION. MUSCLE FIBRES. NERVOUS TISSUE.
40 LIFE CYCLE SELF FERTILIZATION OF EGGS IS THE RULE. CROSS FERTILIZATION MAY OCCUR IF THERE IS MORE THAN ONE WORM. EGGS ARE PASSED IN FAECES. EGGS ARE INGESTED BY INTERMEDIATE HOST WHICH IS USUALLY AN ANIMAL : CATTLE, PIG, FISH DEPENDING ON SPECIES.
41 LIFE CYCLE TYPICAL CESTODE EGG HAS A STRIATED OUTER LAYER. THE EMBRYO HAS SIX HOOKS HENCE THE NAME HEXACANTH. HEXACANTH PENETRATE THE WALL OF THE INTESTINE OF INTERMEDIATE HOST. BECOMES DISTRIBUTED IN THE TISSUES.
42 Taenia Egg
43 LIFE CYCLE IN THE TISSUES DEVELOPS INTO CYSTICERCUS WHICH IS A FLUID FILLED SAC CONTAINING AN INVAGINATED RUDIMENTARY SCOLEX. WHEN RAW FLESH IS EATEN THE CYSTICERCUS DEVELOPS INTO AN ADULT WORM. COOKING KILLS CYSTICERCI.
44 CESTODES
45 TREMATODES FLAT LEAF SHAPED WORMS ( EXCEPT SCHISTOSOMES ). SIZE 2 TO 8 cm. WITH ONE EXCEPTION ALL ARE HERMAPHRODITES ( SCHISTOSOMES ). LIFE SPAN UP TO MANY YEARS. ALL REQUIRE AN INTERMEDIATE HOST FOR TRANSMISSION.
46 MORPHOLOGY INTERMEDIATE HOST IS A FRESH WATER MOLLUSC ( SNAIL ). LUMINAL OR TISSUE PARASITES. TEGUMENT IS SPINY. TWO SUCKERS : ORAL & VENTRAL. BIFURCATE G.I. TRACT. NO ANAL OPENING.
47 TREMATODES
48 TREMATODES MUSCLE AND NERVE TISSUES. EXCRETORY SYSTEM. EGGS ARE LARGE WITH AN OPERCULUM ( LID ). EXCEPT SCHISTOSOMES. SCHISTOSOME EGGS ARE SPINED.
49 LIFE CYCLE EGGS ARE PASSED INTO WATER. OPERCULUM OPENS RELEASING A CILIATED MIRACIDIUM. MIRACIDIUM ENTERS SNAIL. DEVELOPS INTO SPOROCYST. SPOROCYST GIVES RISE TO DAUGHTER SPOROCYST OR REDIA DEPENDING ON SPECIES.
50 LIFE CYCLE CERCARIA DEVELOP IN REDIA OR SPOROCYST. CERCARIA ARE RELEASED INTO WATER. TWO PATHWAYS : 1)- CERCARIA ENTER SKIN OF NEXT HOST. 2)- ENCYST INTO METACERCARIA AND SETTLE ON AQUATIC PLANTS OR CREATURES TO BE INGESTED BY NEXT HOST.
51 TREMATODES
52
53
54 PATHOGENESIS SYMPTOMS MAY BE ABSENT OR MINIMAL ESPECIALLY WITH LUMINAL PARASITES. PATHOLOGY MAY BE INDUCED BY THE PHYSICAL PRESENCE OF THE PARASITE e.g. BLOOD LOSS, MALABSORPTION, INTESTINAL OBSTRUCTION. INFLAMMATION AND TISSUE DEATH.
55 PATHOGENESIS IN CERTAIN CASES THE PATHOLOGY IS MEDIATED THROUGH AN ALLERGIC PROCESS DTH. PARASITIC IFECTIONS MAY BE ACCOMPANIED BY RAISED IgE AND EOSINOPHILIA
56 DIAGNOSIS EXAMINATION OF STOOLS, URINE, BLOOD, CSF BODY SECRETIONS, TISSUE BIOPSY : FOR THE PRESENCE OF PARASITES, CYSTS, EGGS, LARVAE. EOSINOPHILIA, RAISED IgE TOTAL AND SPECIFIC. Specific IgG
57 MANAGEMENT TREATMENT. PREVENTION OF TRANSMISSION. VACCINATION.
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