Prof. Dr. Dr. Alfonso Lampen

Transcription

1 FEDERAL INSTITUTE FOR RISK ASSESSMENT Uptake and transport of nanoparticle in vitro and in vivo Prof. Dr. Dr. Alfonso Lampen

2 Nanoparticles: DIN CEN ISO definition: particles that have at least one dimension between nm different properties compared to the bulk material Consumer Relevance: antimicrobial properties in cooking accessories packaging material dietary supplements desinfectants body hygiene and cosmetics Page 2

3 Silver nanoparticles: Certified reference material - NM-300 Silver (JRC, OECD) - BAM-N001 BAM = Federal Institute for Materials Research and Testing Source: EUR EN 2011 JRC Report NM-Series of Representative Manufactured Nanomaterials NM-300 Silver unpublished data stabilized spherical silver-particles: Figure: TEM characterization of NM-300 material - silver core radius (SAXS) 7,9 ± 0,1nm - hydrodynamic radius (DLS) 14,7 ± 0,2 nm - zeta potential [ph 6 bis 7] -30 mv Page 3

4 In vivo repeated dose study 28 daysrepeateddose (OECD: 407) Wistar rats Group Silver species Dose(mg/kg K.G./day) 1 Ag-Nanoparticle 60 2 Ag-Nanoparticle 6 3 Ag-Ions 9 4 Ag-Ions 0,9 5 coating 6 6 vehicle 7,5 ml/kg 5 (+1) Tier pro Gruppe = 36 Tiere Pictures; silver accumulates in the intestine, in testis, spleen and liver Page 4

5 In vivo single dose study bioavailability -in vivo vs. PBPK - silver was mainly detected in liver and feces (i.v.) silver accumulates in the intestine, in testis, spleen and liver Page 5

6 In vivo single dose study bioavailability -in vivo vs. PBPK - Best correlations between in vivo data and PBPK modeling: for nanoparticle treatment (86% large nanoparticles, 14% ions) and for ionic treatment (27% ions, 56% small nanoparticles, 17% large nanoparticles) De novo formation of silver nanoparticles originating from silver ions occurs after i.v. injection Reference: In vivo distribution of nanosilver in the rat: the role of ions and de novo-formed secondary particles 2016 Food and Chemical Toxicology; accepted Page 6

7 Caco-2 human intestine model intestine colon carcinoma human epithelial after 21 days cells differentiated and polarized widely used transport and barrier model gut lumen blood Transwell System apical Caco-2 cells basolateral Page 7

8 Protein Corona analysis of iron oxide NP via magnetization + LC MS/MS 143 unique proteins originated of several cellular compartments were identified Identified proteins were mainly of low molecular weight, low abundant and of different pi Association between identified proteins and cellular functions? unpublished data Page 8

9 Summary In vivo Silver was mainly detected in liver and feces Silver accumulates in testis, spleen and intestine PBPK modeling supported de novo formation of silver NP after i.v. injection Silver ionic or nanoparticulate is only marginally bioavailable after oral ingestion In vitro Silver was taken up in intestinal cells as a function of the particle coating material Intracellular: associations between silver and sulfur was observed Silver can cross the intestinal barrier in small amounts Analysis of protein corona composition reveal additional information regarding uptake and toxicological mechanism Protein corona composition fits well with observed proteomic alterations after NP treatment Page 9

10 Acknowledgement: Linda Böhmert, Dajana Lichtenstein Sabine Juling FEDERAL INSTITUTE FOR RISK ASSESSMENT Andreas Thünemann, Federal Institute formaterials Research andtesting, BAM, Berlin, Germany Irina Estrela-Lopis, University of Leipzig, Germany Thank you for your attention! Prof. Dr. Dr. Alfonso Lampen Department Food Safetyt Federal Institute for Risk Assessment (BfR)