Replicate #1 Replicate #2 Replicate #3 Units Average Units Average/Area Units
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- Ophelia Mitchell
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1 Group 1: Polypropylene Day 2 Day 16
2 Group 2: Polypropylene Day 4 Day 8
3 Group 3: Low Density Polyethylene Day 2 Day 16
4 Group 4: Low Density Polyethylene Day 4 Day 8
5 Group 5: Water (H2O) Day 1 Day 2
6 Day 4 X X X X X X Group 5 & 6 Combined Data: Water (H2O) Day 4
7 Group 6: Water (H2O) Day 4 X X X X X Day 8
8 Day 16 Group 5 & 6 Combined Data: Water (H2O) Day 4
9 All Groups: Water (H2O) Time Temperature (Days) (Celsius)
10 Group 1: Polypropylene Day CFU 4 CFU 2 CFU/cm 2 Day CFU 86 CFU 43 CFU/cm 2
11 Group 2: Polypropylene Day CFU 23 CFU 11 CFU/cm 2 Day CFU 62 CFU 31 CFU/cm 2
12 Group 3: Low Density Polyethylene Day CFU 6 CFU 3 CFU/cm 2 Day CFU 137 CFU 69 CFU/cm 2
13 Group 4: Low Density Polyethylene Day CFU 57 CFU 29 CFU/cm 2 Day CFU 122 CFU 61 CFU/cm 2
14 Group 5: Water (H2O) Day CFU 65 CFU 33 CFU/ml Day CFU 60 CFU 30 CFU/ml
15 Day 4 44 X X CFU X X X X Group 5 & 6 Combined Data: Water (H2O) Day CFU 45 CFU 22 CFU/ml
16 Group 6: Water (H2O) Day 4 X CFU X X X X Day CFU 24 CFU 12 CFU/ml
17 Day CFU 25 CFU 13 CFU/ml Group 5 & 6 Combined Data: Water (H2O) Day CFU 45 CFU 22 CFU/ml
18 All Groups: Water (H2O) Time Temperature (Days) (Celsius)
19 Name: Discussion Questions: 1. What trend do you see for CFU/cm2 over time? a. Why do you think that is? 2. Are both plastic substrates showing the same trend? 3. What trend do you see for CFU/ml over time? 4. What trend do you see for water temperature over time?
20 5. Could water temperature explain the trend seen for CFU/ml over time? Why or why not? 6. Was our hypothesis correct? Could plastic pollution be harboring and possibly transporting bacteria around in the marine environment?
21 Name: Discussion Questions: 1. What trend do you see for CFU/cm2 over time? They should note that there is an increase in CFU/cm2 over time. a. Why do you think that is? They should mention how biofilms protect and enable more growth for bacteria. 2. Are both plastic substrates showing the same trend? We see the same trend for both plastic substrates but they aren t exactly the same. 3. What trend do you see for CFU/ml over time? They should note that there is general decrease in CFU/cm2 over time. 4. What trend do you see for water temperature over time? They should see a decrease in water temperature. 5. Could water temperature explain the trend seen for CFU/ml over time? Why or why not? Yes, vibrios are temperature dependent.
22 6. Was our hypothesis correct? Could plastic pollution be harboring and possibly transporting bacteria around in the marine environment? We can t say for sure that they are transporting bacteria, but they are certainly serving as a habitat. So it is possible! More research should be done! The take home message should be that plastic is not only bad for the environment in ways we ve already listed, but it can also harbor potentially harmful bacteria.
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