mtorc2 controls actin polymerization required for consolidation of long-term memory
|
|
- Esther Dalton
- 5 years ago
- Views:
Transcription
1 CORRECTION NOTICE Nat. Neurosci.; doi:1.138/nn.3351 mtorc2 controls actin polymerization required for consolidation of long-term memory Wei Huang, Ping Jun Zhu, Shixing Zhang, Hongyi Zhou, Loredana Stoica, Mauricio aliano, Krešimir Krnjević, regg Roman & Mauro Costa-Mattioli In the version of this supplementary file originally posted online, in Supplementary igure 11, the wrong images were provided for total S6K for ig. 1a, β-actin for igures 2a and 3a, actin (bottom) for ig. 5a and p-akt for igure 6a, and the panels corresponding to igures 6a, 6b, 6d and 6e were mislabeled 5b, 5c, 5d and 5e, respectively. The errors have been corrected in this file as of 1 March 21. nature neuroscience
2 Supplementary Information mtorc2 controls actin polymerization required for consolidation of long-term memory Wei Huang 1,2#, Ping Jun Zhu 1,2#, Shixing Zhang 3,4, Hongyi Zhou 1,2, Loredana Stoica 1,5, Mauricio aliano 1, Krešimir Krnjević 6, regg Roman 3,4, Mauro Costa-Mattioli 1,2,5*. 1 Department of Neuroscience, 2 Center on Addiction, Learning & Memory, 5 Department of Molecular and Cell Biology, Baylor College of Medicine, Houston, Texas, Department of Biology and Biochemistry, 4 Biology of Behavior Institute, University of Houston, Houston, Texas, Department of Physiology, Mcill University, Montreal H3 1Y6, Canada #These authors contributed equally to this work. *Address correspondence to: Mauro Costa-Mattioli, costamat@bcm.edu
3 a fepsp slope (% change) 1 4X Hz L-LTP +/+;+/+ WT ( ) +/+;Cre/+ Cre ( ) flox/flox;+/+ loxed ( ) Contextual Time (min) Auditory Percentage freezing b /+;+/+ WT ( ) Cre ( ) Naive +/+;Cre/+ flox/flox;+/+ loxed ( ) 24 h c Percentage freezing Pre-CS CS Supplementary igure 1. L-LTP and fear LTM did not differ between WT, CamKII- Cre and floxed mice. a) Similar L-LTP was induced by four tetanic trains in slices from WT ( +/+;+/+, n = 7) αcamkii-cre ( +/+;Cre/+, n = 6) and floxed mice ( flox/flox;+/+, n = 6) (at 22 min (2,17) =.54, p =.95). b) or contextual fear conditioning, freezing times were determined before the conditioning (Naïve, during 2-min period) and then 24 h after training (during a 3-min period). Similar freezing at 24 h reflects normal contextual LTM in WT (n = 7), αcamkii-cre (n = 7) and floxed (n = 7) mice ( (2,19) =.241, p =.79). c) or auditory fear conditioning, freezing times were measured 24 h post-training either before the onset of the tone (Pre-CS, 2 min) or during the tone presentation (Post-CS, 3 min) (n = 7 per group, (2,19) =.662, p =.94).
4 a WT fb-ko Nissl b Rictor Synaptophysin PSD95 AD67 Control fb-ko Relative protein level (arbituary unit) 1 β-actin Synaptophysin PSD95 Control fb-ko AD67 Supplementary igure 2. Lack of Rictor does not alter gross brain morphology or synaptic markers. a) Nissl-stained hippocampal sections from WT and fb-ko mice. b) Antibodies against synaptophysin (t =.152; p =.885), PSD95 (t =.147; p =.889) and AD67 (t =.289; p =.784) showed no difference in CA1 between WT (n = 3) and fb-ko mice (n = 4).
5 mtorc2 a lutamate APV NBQX Total-Akt b NMDA MK c BDN TrkB-c d Immunoreactivity (% control) β actin 2 * ns * e 3 2 * ns f 3 2 * ns Supplementary igure 3. lutamate, NMDA and BDN activate mtorc2. a-c) Western blots show that, in CA1, glutamate ( μμ, a; (3, 12) = 5.832, * p <.5), NMDA ( μμ, b; (2, 12) = 1.8, * p <.5) or BDN ( ng/ml, c; (2, 6) = 5.493, * p <.5) increased mtorc2-mediated Akt-Ser473 phosphorylation. The results, including effects of selective antagonists APV, MK81 and NBQX (for glutamate), and TrkB-c (for BDN), are summarized in d-f. All comparisons are made with control (untreated) group. APV (aminophosphonovalerate) and MK81 block NMDA receptors; BNQX (dihydroxy-nitro-sulfamoyl-benzoquinoxaline-dione) blocks AMPA-type glutamate receptors. ns = no significance.
6 a Afferent volley (mv) 2 1 Control (n = 26) fb-ko (n = 18) b fepsp slope (v/s) _ Stimulation intensity (V) Afferent volley (mv) c Paired pulse ratio Interpulse intervals (ms) Supplementary igure 4. Basal synaptic transmission in CA1 does not differ between slices from control and fb-ko mice. a) Plots of presynaptic fiber volley as function of stimulus intensity show no difference in fiber excitability between control (n = 26) and fb-ko mice (n = 21). The data were fitted by linear regressions: R 2 =.991 for controls and.9838 for fb-ko mice. b) Input-output plots show similar EPSPs as function of presynaptic fiber volley amplitude over a wide range of stimulus intensities. The corresponding linear regressions are: R 2 =.6983 for controls and.7392 for fb-ko mice. c) Paired-pulse facilitation of fepsps also did not differ between WT and fb-ko mice, as shown by the plots of the PP ratio (fepsp 2 / fepsp 1 ) for various intervals of paired stimulation.
7 Visible-platform Latency (s) Control fb-ko Days Supplementary igure 5. fb-ko mice show normal visuo-motor function. In the visible platform version of the Morris water maze, latencies of escape did not differ between controls and fb-ko mice.
8 a fepsp slope (% change) Vehicle (n=5) JPK (n=5) 2 mv 5 ms JPK ( nm) fb-ko Time (min) b fepsp slope (% change) Vehicle Cyto-D 2 1X Hz 1 Cyto-D ( nm) _ WT _ 1 2 Time (min) 5 ms 2 mv Supplementary igure 6. Control tests of actin polymerization promoter jasplakinolide (JPK) and inhibitor cytochalasin-d (Cyt-D). a) In slices from fb- KO mice, JPK ( nm) had no effect on basal synaptic transmission expressed by fepsps (n = 5 per group, t = 1.81; p =.12). b) In WT slices Cyt-D ( nm) did not affect early-ltp induced by a single high frequency train ( Hz, 1 s). or these plots, n = 5 for controls (vehicle) and 6 for Cyt-D: at 2 min ( (1, 1) =.42, p =.993). Inset: averaged traces were obtained before and after prolonged application of JPK (a) or before and after 2 min after tetanus (b).
9 Control Vehicle JPK ( ng) fb-ko mm mm -2.6 mm -2.6 mm mm mm -2.3 mm -2.3 mm mm mm Supplementary igure 7. Sites of JPK infusion into dorsal hippocampus at five rostrocaudal planes. Coordinates are posterior to bregma and cannula tip placements are from mice infused with JPK (filled squares) and vehicle (filled circles). Below are photomicrographs showing representative cannula tracks into the dorsal hippocampus.
10 a actin Vehicle JPK Vehicle JPK actin -actin/-actin ratio (arbituary unit) * Vehicle fb-ko JPK Vehicle JPK WT b 6 Auditory Percentage freezing 4 2 Vehicle JPK Pre-CS CS WT Supplementary igure 8. A low dose of JPK infused into the hippocampus promotes actin polymerization in fb-ko mice. a) Western blots show that thirty min after infusion of JPK ( ng/ml) in the dorsal hippocampus from fb-ko mice the -actin/-actin ratio was increased (at left; n = 4, t = 2.942; * p <.5), but not in the dorsal hippocampus from WT mice (at right; n = 3, t = 1.57; p =.187). Normalized data are shown below. b) Intra-hippocampal infusion of JPK immediately after a weak training (a single pairing of a tone with a 1 s,.7 ma foot-shock) had no effect on auditory fear LTM (n = 15 for vehicle and n = 16 for JPK; H =.768, ANOVA on Ranks, p =.78)
11 a fepsp slope (% change) 1 1X Hz _ JPK nm Aniso 4 µμ WT _ 1 2 Time (min) JPK (n=8) JPK + Aniso (n=6) b fepsp slope (% change) 1 4X Hz Aniso (n=4) JPK + Aniso (n=5) Aniso 4 µμ _ JPK nm WT _ 1 2 Time (min) Supplementary igure 9. Late-LTP induced by a single tetanic stimulation in combination with JPK is dependent on mrna translation. a) The facilitated L-LTP induced by a single tetanus in the presence of JPK (nm) was suppressed by anisomycin (Aniso 4 µm, at 18 min: 79 ± 12.7% for JPK and 17 ± 4.1% for JPK + Aniso, H = 9.6, p <.1). b) JPK ( nm) had no effect on the impaired L-LTP induced by four tetanic trains in the presence of anisomycin (4 µm, at 22 min: 27 ± 8.6% for Aniso and 33 ± 11.4% for JPK + Aniso, (1, 7) =.5, p =.945).
12 mtorc2 p-pak (Ser198/23) Total PAK β-actin Vehicle A Immunoreactivity ( % control) p-pak (Ser198/23) 3 ** 2 ** 2 Vehicle A Vehicle A Supplementary igure 1. A promotes mtorc2 activity in the hippocampus in vivo. Western blots show that 3 min after systemic injection of A (2.5 mg/kg, i.p.) mtorc2-mediated phosphorylation of both Akt (Ser473) and PAK (Ser198/23) were increased in dorsal hippocampus (bottom left, n = 3 per group, t = 5.726, ** p <.1; bottom right, n = 3 per group, t = 8.263; ** p <.1).
13 ig. 1a 2 1 Rictor ig. 1c 2 1 Rictor 2 Rictor ig. 1b p-s6k (Thr389) p-s6k (Thr389) Total S6K ig. 1f Total S6K p-s6k (Thr389) Total S6K Control 4 x Hz ig. 1h ig. 1e Control 1 x Hz p-pak (Ser198/23) ig. 2a Time after training (min) ig. 2c Total PAK Control 4 x Hz Time after training (min) H om C ec on ag Sh text e oc -al k- on al e on e Supplementary igure 11. Images of full length blots presented in the main figures.
14 r 1 ig. 4a ri c p-dakt (Ser5) ig. 4c Control fb-ko Actin Pull down ig. 4e ig. 4i Lysate IP: lag DH PDZ L DH PDZ L IP: lag *Heavy chain DH PDZ L DH PDZ L 15 lag lag *Heavy chain myc 2 1 ig. 4j lysate ig. 6a Actin Ve hi A- cle IP: myc DH PDZ L lag WT Vehicle JPK * p-pak (Ser198/23) ig. 6d Vehicle A Actin ig. 6e Vehicle A Actin p-pak (Ser198/23) Total PAK Actin ig. 6b Ve hi A- cle Ve hi A- cle fb-ko Vehicle JPK 2 1 Rictor ig. 5a Lysate IP LysTiam1 Ig Tiam1 lag Total Cofilin ig. 4k IP: myc DH PDZ L p-cofilin (Ser3) ig. 4j 2 1 Total PAK 25 2 Cdc42 myc p-pak (Ser198/23) Lysate Control fb-ko Control fb-ko 25 2 Total dakt Control fb-ko 25 Rac1 2 Rac1 Lysate Pull down Control fb-ko Ve hi A- cle C an to to n -S ig. 3a Total PAK Supplementary igure 11(continued). Images of full length blots presented in the main figures.
SUPPLEMENTARY INFORMATION
Supplementary Figure 1. Normal AMPAR-mediated fepsp input-output curve in CA3-Psen cdko mice. Input-output curves, which are plotted initial slopes of the evoked fepsp as function of the amplitude of the
More informationSUPPLEMENTARY INFORMATION
Supplementary Figure 1. Behavioural effects of ketamine in non-stressed and stressed mice. Naive C57BL/6 adult male mice (n=10/group) were given a single dose of saline vehicle or ketamine (3.0 mg/kg,
More informationSupplemental information Acid-sensing ion channel 1a contributes to hippocampal LTP inducibility through multiple mechanisms
Supplemental information Acid-sensing ion channel 1a contributes to hippocampal LTP inducibility through multiple mechanisms Ming-Gang Liu, Hu-Song Li, Wei-Guang Li, Yan-Jiao Wu, Shi-Ning Deng, Chen Huang,
More informationSynaptic plasticityhippocampus. Neur 8790 Topics in Neuroscience: Neuroplasticity. Outline. Synaptic plasticity hypothesis
Synaptic plasticityhippocampus Neur 8790 Topics in Neuroscience: Neuroplasticity Outline Synaptic plasticity hypothesis Long term potentiation in the hippocampus How it s measured What it looks like Mechanisms
More informationSynaptic Plasticity and NO-cGMP-PKG Signaling Regulate Pre- and Postsynaptic Alterations at Rat Lateral Amygdala Synapses Following Fear Conditioning
Synaptic Plasticity and NO-cGMP-PKG Signaling Regulate Pre- and Postsynaptic Alterations at Rat Lateral Amygdala Synapses Following Fear Conditioning Kristie T. Ota 1, Melissa S. Monsey 1, Melissa S. Wu
More informationBIPN140 Lecture 12: Synaptic Plasticity (II)
BIPN140 Lecture 12: Synaptic Plasticity (II) 1. Early v.s. Late LTP 2. Long-Term Depression 3. Molecular Mechanisms of Long-Term Depression: NMDA-R dependent 4. Molecular Mechanisms of Long-Term Depression:
More informationSUPPLEMENTARY INFORMATION
doi:10.1038/nature09667 A critical role for IGF-II in memory consolidation and enhancement Dillon Y. Chen, Sarah A. Stern, Ana Garcia-Osta, Bernadette Saunier-Rebori, Gabriella Pollonini, Dhananjay Bambah-Mukku,
More informationSUPPLEMENTARY INFORMATION. Rett Syndrome Mutation MeCP2 T158A Disrupts DNA Binding, Protein Stability and ERP Responses
SUPPLEMENTARY INFORMATION Rett Syndrome Mutation T158A Disrupts DNA Binding, Protein Stability and ERP Responses Darren Goffin, Megan Allen, Le Zhang, Maria Amorim, I-Ting Judy Wang, Arith-Ruth S. Reyes,
More informationCellular Neurobiology BIPN140
Cellular Neurobiology BIPN140 1st Midterm Exam Ready for Pickup By the elevator on the 3 rd Floor of Pacific Hall (waiver) Exam Depot Window at the north entrance to Pacific Hall (no waiver) Mon-Fri, 10:00
More informationSupplementary Information
Supplementary Information D-Serine regulates cerebellar LTD and motor coordination through the 2 glutamate receptor Wataru Kakegawa, Yurika Miyoshi, Kenji Hamase, Shinji Matsuda, Keiko Matsuda, Kazuhisa
More informationBIPN 140 Problem Set 6
BIPN 140 Problem Set 6 1) The hippocampus is a cortical structure in the medial portion of the temporal lobe (medial temporal lobe in primates. a) What is the main function of the hippocampus? The hippocampus
More informationBIPN 140 Problem Set 6
BIPN 140 Problem Set 6 1) Hippocampus is a cortical structure in the medial portion of the temporal lobe (medial temporal lobe in primates. a) What is the main function of the hippocampus? The hippocampus
More informationSupplementary Figure 1. Basic properties of compound EPSPs at
Supplementary Figure 1. Basic properties of compound EPSPs at hippocampal CA3 CA3 cell synapses. (a) EPSPs were evoked by extracellular stimulation of the recurrent collaterals and pharmacologically isolated
More informationSUPPLEMENTARY INFORMATION
doi: 10.1038/nature05772 SUPPLEMENTARY INFORMATION Supplemental figure 1. Enrichment facilitates learning. a. Images showing a home cage and a cage used for environmental enrichment (EE). For EE up to
More informationBidirectional NMDA receptor plasticity controls CA3 output and heterosynaptic metaplasticity
Bidirectional NMDA receptor plasticity controls CA output and heterosynaptic metaplasticity David L. Hunt, Nagore Puente, Pedro Grandes, Pablo E. Castillo a NMDAR EPSC (pa) - - -8-6 -4 - st 5 nd 5 b NMDAR
More informationNature Neuroscience: doi: /nn Supplementary Figure 1
Supplementary Figure 1 Bidirectional optogenetic modulation of the tonic activity of CEA PKCδ + neurons in vitro. a, Top, Cell-attached voltage recording illustrating the blue light-induced increase in
More informationSUPPLEMENTARY INFORMATION. Supplementary Figure 1
SUPPLEMENTARY INFORMATION Supplementary Figure 1 The supralinear events evoked in CA3 pyramidal cells fulfill the criteria for NMDA spikes, exhibiting a threshold, sensitivity to NMDAR blockade, and all-or-none
More informationRescue of mutant rhodopsin traffic by metformin-induced AMPK activation accelerates photoreceptor degeneration Athanasiou et al
Supplementary Material Rescue of mutant rhodopsin traffic by metformin-induced AMPK activation accelerates photoreceptor degeneration Athanasiou et al Supplementary Figure 1. AICAR improves P23H rod opsin
More informationBehavioral Neuroscience: Fear thou not. Rony Paz
Behavioral Neuroscience: Fear thou not Rony Paz Rony.paz@weizmann.ac.il Thoughts What is a reward? Learning is best motivated by threats to survival Threats are much better reinforcers Fear is a prime
More informationKey words: fear conditioning; APV; ifenprodil; verapamil; LTP; amygdala
The Journal of Neuroscience, June 15, 2002, 22(12):5239 5249 NMDA Receptors and L-Type Voltage-Gated Calcium Channels Contribute to Long-Term Potentiation and Different Components of Fear Memory Formation
More informationSuppl. Information Supplementary Figure 1. Strategy/latency analysis of individual mice during maze learning. a,
Goal-oriented searching mediated by ventral hippocampus early in trial-and-error learning Ruediger, S, Spirig, D., Donato, F., Caroni, P. Suppl. Information Supplementary Figure 1. Strategy/latency analysis
More informationUbe3a is required for experience-dependent maturation of the neocortex
Ube3a is required for experience-dependent maturation of the neocortex Koji Yashiro, Thorfinn T. Riday, Kathryn H. Condon, Adam C. Roberts, Danilo R. Bernardo, Rohit Prakash, Richard J. Weinberg, Michael
More informationCellular mechanisms of information transfer: neuronal and synaptic plasticity
Cellular mechanisms of information transfer: neuronal and synaptic plasticity Ivan Pavlov (UCL Institute of Neurology, UK) Anton Chizhov (Ioffe Physical Technical Institute) Pavel Zykin (St.-Petersburg
More informationShort- and long-lasting consequences of in vivo nicotine treatment
Short- and long-lasting consequences of in vivo nicotine treatment on hippocampal excitability Rachel E. Penton, Michael W. Quick, Robin A. J. Lester Supplementary Figure 1. Histogram showing the maximal
More informationSupporting Online Material for
www.sciencemag.org/cgi/content/full/312/5779/1533/dc1 Supporting Online Material for Long-Term Potentiation of Neuron-Glia Synapses Mediated by Ca 2+ - Permeable AMPA Receptors Woo-Ping Ge, Xiu-Juan Yang,
More informationBMI risk SNPs associate with increased CADM1 and CADM2 expression in the cerebellum of human subjects.
Supplementary Figure 1 BMI risk SNPs associate with increased CADM1 and CADM2 expression in the cerebellum of human subjects. Boxplots show the 25% and 75% quantiles of normalized mrna expression levels
More information1.0. FSL NMDAR-fEPSP 0.8. amplitude (mv) Intensity (µa) 2.0 SD FSL Time (ms)
a 2.5 1. AMPAR-fEPSP slope (mv/ms) 2. 1. NMDAR-fEPSP amplitude (mv).8.6.4.5.2. 2 4 6 8. 1 2 3 4 5 Intensity (µa) Intensity (µa) b 2. PPF Ratio (fepsp2/fepsp1) 1..5. 5 1 2 5 Time (ms) Supplementary Figure
More informationAstrocyte signaling controls spike timing-dependent depression at neocortical synapses
Supplementary Information Astrocyte signaling controls spike timing-dependent depression at neocortical synapses Rogier Min and Thomas Nevian Department of Physiology, University of Berne, Bern, Switzerland
More informationPersistent improvement in synaptic and cognitive functions in an Alzheimer mouse model after rolipram treatment
Persistent improvement in synaptic and cognitive functions in an Alzheimer mouse model after rolipram treatment Bing Gong,, Michael Shelanski, Ottavio Arancio J Clin Invest. 2004;114(11):1624-1634. https://doi.org/10.1172/jci22831.
More informationThe molecular analysis of long-term plasticity in the mammalian
Genetic evidence for a protein-kinase-a-mediated presynaptic component in NMDA-receptor-dependent forms of long-term synaptic potentiation Yan-You Huang*, Stanislav S. Zakharenko*, Susanne Schoch, Pascal
More informationJLC Lee: Memory reconsolidation mediates the strengthening of memories by additional learning
JLC Lee: Memory reconsolidation mediates the strengthening of memories by additional learning 8 6 4 Cond1 Cond2 Test VEH ANI Session Supplementary Fig. 1. Protein synthesis inhibition after a second contextual
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Trial structure for go/no-go behavior
Supplementary Figure 1 Trial structure for go/no-go behavior a, Overall timeline of experiments. Day 1: A1 mapping, injection of AAV1-SYN-GCAMP6s, cranial window and headpost implantation. Water restriction
More informationSUPPLEMENTARY INFORMATION
doi: 10.1038/nature06994 A phosphatase cascade by which rewarding stimuli control nucleosomal response A. Stipanovich*, E. Valjent*, M. Matamales*, A. Nishi, J.H. Ahn, M. Maroteaux, J. Bertran-Gonzalez,
More informationNature Immunology: doi: /ni.3866
Nature Immunology: doi:10.1038/ni.3866 Supplementary Figure 1 The effect of TIPE2 on chemotaxis. a, The expression of TIPE2 in dhl-60c, dhl-60t, TIPE2-expressing and 15/16Q-expressing dhl-60t neutrophils
More informationBehavioral Neuroscience: Fear thou not. Rony Paz
Behavioral Neuroscience: Fear thou not Rony Paz Rony.paz@weizmann.ac.il Thoughts What is a reward? Learning is best motivated by threats to survival? Threats are much better reinforcers? Fear is a prime
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Large-scale calcium imaging in vivo.
Supplementary Figure 1 Large-scale calcium imaging in vivo. (a) Schematic illustration of the in vivo camera imaging set-up for large-scale calcium imaging. (b) High-magnification two-photon image from
More informationLack of GPR88 enhances medium spiny neuron activity and alters. motor- and cue- dependent behaviors
Lack of GPR88 enhances medium spiny neuron activity and alters motor- and cue- dependent behaviors Albert Quintana, Elisenda Sanz, Wengang Wang, Granville P. Storey, Ali D. Güler Matthew J. Wanat, Bryan
More informationSocial deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition
SUPPLEMENTARY INFORMATION Articles https://doi.org/10.1038/s41593-018-0110-8 In the format provided by the authors and unedited. Social deficits in Shank3-deficient mouse models of autism are rescued by
More informationRodent Behavioral Learning and Memory Models. From Mechanisms of Memory, 2 nd Edition by J. David Sweatt, Ph.D.
Rodent Behavioral Learning and Memory Models From Mechanisms of Memory, 2 nd Edition by J. David Sweatt, Ph.D. Hippocampal Pyramidal Neuron of Mice and Rats Figure 1 Open Field Apparatus Open Field Behavior
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/7/308/ra4/dc1 Supplementary Materials for Antipsychotics Activate mtorc1-dependent Translation to Enhance Neuronal Morphological Complexity Heather Bowling, Guoan
More informationSupplementary Materials for
www.sciencesignaling.org/cgi/content/full/6/283/ra57/dc1 Supplementary Materials for JNK3 Couples the Neuronal Stress Response to Inhibition of Secretory Trafficking Guang Yang,* Xun Zhou, Jingyan Zhu,
More informationDNA and Histone Methylation in Learning and Memory
EXPERIMENTAL BIOLOGY ANNUAL MEETING April 2010 DNA and Histone Methylation in Learning and Memory J. David Sweatt Dept of Neurobiology McKnight Brain Institute UAB School of Medicine The Molecular Basis
More informationSupplementary Figure 1
Combination index (CI) Supplementary Figure 1 2. 1.5 1. Ishikawa AN3CA Nou-1 Hec-18.5...2.4.6.8 1. Fraction affected (Fa) Supplementary Figure 1. The synergistic effect of PARP inhibitor and PI3K inhibitor
More informationSystems Neuroscience November 29, Memory
Systems Neuroscience November 29, 2016 Memory Gabriela Michel http: www.ini.unizh.ch/~kiper/system_neurosci.html Forms of memory Different types of learning & memory rely on different brain structures
More informationSupplementary Figure 1. SybII and Ceb are sorted to distinct vesicle populations in astrocytes. Nature Neuroscience: doi: /nn.
Supplementary Figure 1 SybII and Ceb are sorted to distinct vesicle populations in astrocytes. (a) Exemplary images for cultured astrocytes co-immunolabeled with SybII and Ceb antibodies. SybII accumulates
More informationModulation of TRP channels by resolvins in mouse and human
July 9, 2015, Ion Channel Retreat, Vancouver Ion Channel and Pain Targets Modulation of TRP channels by resolvins in mouse and human Ru-Rong Ji, PhD Pain Research Division Department of Anesthesiology
More informationEthanol-mediated long-lasting adaptations of the NR2B-containing NMDA receptors in the dorsomedial striatum
Article Addendum Channels 5:3, 205-209; May/June 2011; 2011 Landes Bioscience Article Addendum Ethanol-mediated long-lasting adaptations of the NR2B-containing NMDA receptors in the dorsomedial striatum
More informationSupplementary Figure 1. Microglia do not show signs of classical immune activation following MD a-b. Images showing immunoreactivity for MHCII (a)
1 Supplementary Figure 1. Microglia do not show signs of classical immune activation following MD a-b. Images showing immunoreactivity for MHCII (a) and CD45 (b) in fixed sections of binocular visual cortex
More informationSynaptic Plasticity and Memory
Synaptic Plasticity and Memory Properties and synaptic mechanisms underlying the induction of long-term potentiation (LTP) The role of calcium/calmodulin-dependent kinase II (CamKII) in the induction,
More informationCdk5 Is Required for Memory Function and Hippocampal Plasticity via the camp Signaling Pathway
Cdk5 Is Required for Memory Function and Hippocampal Plasticity via the camp Signaling Pathway Ji-Song Guan 1,2,3,4., Susan C. Su 1,2,3,4., Jun Gao 1,2,3,4, Nadine Joseph 1,2,3,4, Zhigang Xie 1,2, Ying
More informationThe Neurobiology of Learning and Memory
The Neurobiology of Learning and Memory JERRY W. RUDY University of Colorado, Boulder Sinauer Associates, Inc. Publishers Sunderland, Massachusetts 01375 Table of Contents CHAPTER 1 Introduction: Fundamental
More informationAuthors: K. L. Arendt, M. Royo, M. Fernández-Monreal, S. Knafo, C. N. Petrok, J.
SUPPLEMENTARY INFORMATION Title: PIP 3 controls synaptic function by maintaining AMPA receptor clustering at the postsynaptic membrane Authors: K. L. Arendt, M. Royo, M. Fernández-Monreal, S. Knafo, C.
More informationSupplementary Figure 1. mir124 does not change neuron morphology and synaptic
Supplementary Figure 1. mir124 does not change neuron morphology and synaptic density. Hippocampal neurons were transfected with mir124 (containing DsRed) or DsRed as a control. 2 d after transfection,
More informationTitle: Plasticity of intrinsic excitability in mature granule cells of the dentate gyrus
Title: Plasticity of intrinsic excitability in mature granule cells of the dentate gyrus Authors: Jeffrey Lopez-Rojas a1, Martin Heine b1 and Michael R. Kreutz ac1 a Research Group Neuroplasticity, b Research
More informationCellular Neurobiology / BIPN 140
SECOND MIDTERM EXAMINATION Fall, 2015 GENERAL INSTRUCTIONS 1. Please write your name on ALL 6 pages. 2. Please answer each question IN THE SPACE ALLOTTED. 1) /10 pts 2) /10 pts 3) /15 pts 4) /15 pts 5)
More informationRoles of NMDA NR2B Subtype Receptor in Prefrontal Long-Term Potentiation and Contextual Fear Memory
Neuron, Vol. 47, 859 872, September 15, 2005, Copyright 2005 by Elsevier Inc. DOI 10.1016/j.neuron.2005.08.014 Roles of NMDA NR2B Subtype Receptor in Prefrontal Long-Term Potentiation and Contextual Fear
More informationSupplementary Figure 1
Supplementary Figure 1 Miniature microdrive, spike sorting and sleep stage detection. a, A movable recording probe with 8-tetrodes (32-channels). It weighs ~1g. b, A mouse implanted with 8 tetrodes in
More information9.01 Introduction to Neuroscience Fall 2007
MIT OpenCourseWare http://ocw.mit.edu 9.01 Introduction to Neuroscience Fall 2007 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms. Declarative memory conscious,
More informationRescue of neurological deficits in a mouse model for Angelman Syndrome by reduction of αcamkii inhibitory phosphorylation
Rescue of neurological deficits in a mouse model for Angelman Syndrome by reduction of αcamkii inhibitory phosphorylation Geeske M van Woerden 1, Karen D Harris 2, Mohammad Reza Hojjati 1, Richard M Gustin
More informationBasal Ganglia Anatomy, Physiology, and Function. NS201c
Basal Ganglia Anatomy, Physiology, and Function NS201c Human Basal Ganglia Anatomy Basal Ganglia Circuits: The Classical Model of Direct and Indirect Pathway Function Motor Cortex Premotor Cortex + Glutamate
More informationLearning and Memory. The Case of H.M.
Learning and Memory Learning deals with how experience changes the brain Memory refers to how these changes are stored and later reactivated The Case of H.M. H.M. suffered from severe, intractable epilepsy
More informationSUPPLEMENTARY INFORMATION
doi: 1.138/nature6416 Supplementary Notes Spine Ca 2+ signals produced by glutamate uncaging We imaged uncaging-evoked [Ca 2+ ] transients in neurons loaded with a green Ca 2+ - sensitive indicator (G;
More informationc Ischemia (30 min) Reperfusion (8 w) Supplementary Figure bp 300 bp Ischemia (30 min) Reperfusion (4 h) Dox 20 mg/kg i.p.
a Marker Ripk3 +/ 5 bp 3 bp b Ischemia (3 min) Reperfusion (4 h) d 2 mg/kg i.p. 1 w 5 w Sacrifice for IF size A subset for echocardiography and morphological analysis c Ischemia (3 min) Reperfusion (8
More informationPhosphatidylinositol 3-Kinase Regulates the Induction of Long-Term Potentiation through Extracellular Signal- Related Kinase-Independent Mechanisms
The Journal of Neuroscience, May 1, 2003 23(9):3679 3688 3679 Phosphatidylinositol 3-Kinase Regulates the Induction of Long-Term Potentiation through Extracellular Signal- Related Kinase-Independent Mechanisms
More informationPKMζ maintains memories by regulating GluR2- dependent AMPA receptor trafficking
Supplementary information PKMζ maintains memories by regulating GluR2- dependent AMPA receptor trafficking Paola Virginia Migues, Oliver Hardt, Dong Chuan Wu, Karine Gamache, Todd Charlton Sacktor, Yu
More informationSupporting Information
ATP from synaptic terminals and astrocytes regulates NMDA receptors and synaptic plasticity through PSD- 95 multi- protein complex U.Lalo, O.Palygin, A.Verkhratsky, S.G.N. Grant and Y. Pankratov Supporting
More informationSupplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1. Short latency of the fepsp evoked in CA3 by electrical stimulation of perforant path inputs (a) Single and superimposed representative perforant pathway-ca3
More informationSupplementary Figures
Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein
More informationNature Biotechnology: doi: /nbt Supplementary Figure 1. Analysis of hair bundle morphology in Ush1c c.216g>a mice at P18 by SEM.
Supplementary Figure 1 Analysis of hair bundle morphology in Ush1c c.216g>a mice at P18 by SEM. (a-c) Heterozygous c.216ga mice displayed normal hair bundle morphology at P18. (d-i) Disorganized hair bundles
More informationa! b! c! Supplementary Fig. 1! Diameter (μm) S1P (LogM) U (LogM) enos! β-actin! Nogo-B! MLEC! nnos! β-actin!
a! b! c! Diamet (μm) 2 2 1 WT Nogo-A/B-deficient -9-8 -7 - -5-4 PE (LogM) Diamet (μm) 2 2 1-12-11-1 -9-8 -7 - -5 U-419 (LogM) Diamet (μm) 2 2 1-8 -7 - -5 S1P (LogM) d! WT! Nogo-A/B-deficient! MLE! enos!
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Diverse anorexigenic signals induce c-fos expression in CEl PKC-δ + neurons
Supplementary Figure 1 Diverse anorexigenic signals induce c-fos expression in CEl PKC-δ + neurons a-c. Quantification of CEl c-fos expression in mice intraperitoneal injected with anorexigenic drugs (a),
More informationSuppression of PKR Promotes Network Excitability and Enhanced Cognition by Interferon-g-Mediated Disinhibition
Suppression of PKR Promotes Network Excitability and Enhanced Cognition by Interferon-g-Mediated Disinhibition Ping Jun Zhu, 1, Wei Huang, 1, Djanenkhodja Kalikulov, 1,7 Jong W. Yoo, 3 Andon N. Placzek,
More informationSupplemental Information. Menin Deficiency Leads to Depressive-like. Behaviors in Mice by Modulating. Astrocyte-Mediated Neuroinflammation
Neuron, Volume 100 Supplemental Information Menin Deficiency Leads to Depressive-like Behaviors in Mice by Modulating Astrocyte-Mediated Neuroinflammation Lige Leng, Kai Zhuang, Zeyue Liu, Changquan Huang,
More informationHypoxia-Induced Neonatal Seizures Diminish Silent Synapses and Long-Term Potentiation in Hippocampal CA1 Neurons
The Journal of Neuroscience, December 14, 2011 31(50):18211 18222 18211 Cellular/Molecular Hypoxia-Induced Neonatal Seizures Diminish Silent Synapses and Long-Term Potentiation in Hippocampal CA1 Neurons
More informationSupplementary Figure 1) GABAergic enhancement by leptin hyperpolarizes POMC neurons A) Representative recording samples showing the membrane
Supplementary Figure 1) GABAergic enhancement by leptin hyperpolarizes POMC neurons A) Representative recording samples showing the membrane potential recorded from POMC neurons following treatment with
More informationMemory Systems II How Stored: Engram and LTP. Reading: BCP Chapter 25
Memory Systems II How Stored: Engram and LTP Reading: BCP Chapter 25 Memory Systems Learning is the acquisition of new knowledge or skills. Memory is the retention of learned information. Many different
More informationIntroduction to Physiological Psychology Learning and Memory II
Introduction to Physiological Psychology Learning and Memory II ksweeney@cogsci.ucsd.edu cogsci.ucsd.edu/~ksweeney/psy260.html Memory Working Memory Long-term Memory Declarative Memory Procedural Memory
More informationRegulation of Learning by EphA Receptors: a Protein Targeting Study
The Journal of Neuroscience, November 1, 1999, 19(21):9538 9549 Regulation of Learning by EphA Receptors: a Protein Targeting Study R. Gerlai, 1 N. Shinsky, 1 A. Shih, 1 P. Williams, 2 J. Winer, 2 M. Armanini,
More informationBidirectional modifications in synaptic efficacy, exemplified
Capture of a protein synthesis-dependent component of long-term depression Beth S. Kauderer* and Eric R. Kandel* Howard Hughes Medical Institute and *Center for Neurobiology and Behavior, College of Physicians
More informationDopamine in Ube3a m-/p+ mice. Online Supplemental Material
Online Supplemental Material S1 Supplemental Figure 1. Schematic of rate-dependent intracranial self-stimulation (ICSS) (A) Mice implanted with monopolar stimulating electrodes to the medial forebrain
More informationSupplemental Figure 1. Western blot analysis indicated that MIF was detected in the fractions of
Supplemental Figure Legends Supplemental Figure 1. Western blot analysis indicated that was detected in the fractions of plasma membrane and cytosol but not in nuclear fraction isolated from Pkd1 null
More informationSupplementary Material
Supplementary Material The Androgen Receptor is a negative regulator of eif4e Phosphorylation at S209: Implications for the use of mtor inhibitors in advanced prostate cancer Supplementary Figures Supplemental
More informationSupplementary Figure 1
8w Pia II/III IV V VI PV EYFP EYFP PV EYFP PV d PV EYFP Supplementary Figure a Spike probability x - PV-Cre d Spike probability x - RS RS b e Spike probability Spike probability.6......8..... FS FS c f
More informationA form of long-lasting, learning-related synaptic plasticity in the hippocampus induced by heterosynaptic low-frequency pairing
A form of long-lasting, learning-related synaptic plasticity in the hippocampus induced by heterosynaptic low-frequency pairing Yan-You Huang, Christopher Pittenger*, and Eric R. Kandel Center for Neurobiology
More informationDep. Control Time (min)
aa Control Dep. RP 1s 1 mv 2s 1 mv b % potentiation of IPSP 2 15 1 5 Dep. * 1 2 3 4 Time (min) Supplementary Figure 1. Rebound potentiation of IPSPs in PCs. a, IPSPs recorded with a K + gluconate pipette
More informationSynaptic Transmission: Ionic and Metabotropic
Synaptic Transmission: Ionic and Metabotropic D. Purves et al. Neuroscience (Sinauer Assoc.) Chapters 5, 6, 7. C. Koch. Biophysics of Computation (Oxford) Chapter 4. J.G. Nicholls et al. From Neuron to
More informationSupplementary Figure 1. Western blot of hippocampal lysates from WT and Adcy1 KO mice demonstrates the specificity of the ADCY1 antibody.
ADCY1 13 kda β-actin 45 kda Supplementary Figure 1. Western blot of hippocampal lysates from and mice demonstrates the specificity of the ADCY1 antibody. a DHPG perk1/2 ERK1/2 Relative level min 1.6 *
More informationSupplementary Fig. 1. GPRC5A post-transcriptionally down-regulates EGFR expression. (a) Plot of the changes in steady state mrna levels versus
Supplementary Fig. 1. GPRC5A post-transcriptionally down-regulates EGFR expression. (a) Plot of the changes in steady state mrna levels versus changes in corresponding proteins between wild type and Gprc5a-/-
More informationSupplementary Figure 1. EC-specific Deletion of Snail1 Does Not Affect EC Apoptosis. (a,b) Cryo-sections of WT (a) and Snail1 LOF (b) embryos at
Supplementary Figure 1. EC-specific Deletion of Snail1 Does Not Affect EC Apoptosis. (a,b) Cryo-sections of WT (a) and Snail1 LOF (b) embryos at E10.5 were double-stained for TUNEL (red) and PECAM-1 (green).
More informationCONTEXT. LTP (long term potentiation) definition. LTP as a interesting mechanism for learning and memory
CONTEXT LTP (long term potentiation) definition LTP as a interesting mechanism for learning and memory LTP is due primarily to a pre or post- synaptic modification? (Increased Glut release or increased
More informationGABA B Receptor-Mediated Presynaptic Inhibition Has History-Dependent Effects on Synaptic Transmission during Physiologically Relevant Spike Trains
The Journal of Neuroscience, June 15, 2003 23(12):4809 4814 4809 Brief Communication GABA B Receptor-Mediated Presynaptic Inhibition Has History-Dependent Effects on Synaptic Transmission during Physiologically
More informationSupporting Online Material for
www.sciencemag.org/cgi/content/full/317/5841/183/dc1 Supporting Online Material for Astrocytes Potentiate Transmitter Release at Single Hippocampal Synapses Gertrudis Perea and Alfonso Araque* *To whom
More informationCellular and Molecular Mechanisms of Learning and Memory
Cellular/Molecular Mechanisms of Learning and Memory 27 Cellular and Molecular Mechanisms of Learning and Memory 2 Matthew Lattal and Ted Abel The nature of the cellular basis of learning and memory remains
More informationExpanded View Figures
Shao-Ming Shen et al Role of I in MT of cancers MO reports xpanded View igures igure V1. nalysis of the expression of I isoforms in cancer cells and their interaction with PTN. RT PR detection of Ish and
More informationTargeting the p75 Receptor to Inhibit Degenerative Signaling and Tau Phosphorylation/Misfolding/ Missorting: Preclinical through Phase 1
Targeting the p75 Receptor to Inhibit Degenerative Signaling and Tau Phosphorylation/Misfolding/ Missorting: Preclinical through Phase 1 ADC Directors Meeting, April 18 th 2015 Frank M. Longo, Stanford
More informationNeuron-Derived Estrogen Regulates Synaptic Plasticity and Memory
This Accepted Manuscript has not been copyedited and formatted. The final version may differ from this version. Research Articles: Cellular/Molecular Neuron-Derived Estrogen Regulates Synaptic Plasticity
More informationSupplementary Figure 1
Supplementary Figure 1 Global TeNT expression effectively impairs synaptic transmission. Injection of 100 pg tent mrna leads to a reduction of vesicle mediated synaptic transmission in the spinal cord
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2566 Figure S1 CDKL5 protein expression pattern and localization in mouse brain. (a) Multiple-tissue western blot from a postnatal day (P) 21 mouse probed with an antibody against CDKL5.
More informationTwo distinct mechanisms for experiencedependent
SUPPLEMENTARY INFORMATION Articles https://doi.org/10.1038/s41593-018-0150-0 In the format provided by the authors and unedited. Two distinct mechanisms for experiencedependent homeostasis Michelle C.
More information