Concept 8.3: ATP powers cellular work by coupling exergonic reactions to endergonic reactions

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1 Concept 8.3: ATP powers cellular work by coupling exergonic reactions to endergonic reactions A cell does three main kinds of work: Chemical Transport Mechanical To do work, cells manage energy resources by energy coupling, the use of an exergonic process to drive an endergonic one Most energy coupling in cells is mediated by ATP Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

2 The Structure and Hydrolysis of ATP ATP (adenosine triphosphate) is the cell s energy shuttle ATP is composed of ribose (a sugar), adenine (a nitrogenous base), and three phosphate groups Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

3 Fig. 8-8 Adenine Phosphate groups Ribose

4 The bonds between the phosphate groups of ATP s tail can be broken by hydrolysis Energy is released from ATP when the terminal phosphate bond is broken This release of energy comes from the chemical change to a state of lower free energy, not from the phosphate bonds themselves Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

5 Fig. 8-9 P P P Adenosine triphosphate (ATP) H 2 O P + P P + i Energy Inorganic phosphate Adenosine diphosphate (ADP)

6 How ATP Performs Work The three types of cellular work (mechanical, transport, and chemical) are powered by the hydrolysis of ATP In the cell, the energy from the exergonic reaction of ATP hydrolysis can be used to drive an endergonic reaction Overall, the coupled reactions are exergonic Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

7 Fig NH 2 Glu Glutamic acid + NH 3 Ammonia (a) Endergonic reaction Glu Glutamine G = +3.4 kcal/mol 1 ATP phosphorylates glutamic acid, making the amino acid less stable. Glu + ATP Glu P + ADP NH 2 2 Ammonia displaces the phosphate group, forming glutamine. Glu P + NH 3 Glu + P i (b) Coupled with ATP hydrolysis, an exergonic reaction (c) Overall free-energy change

8 ATP drives endergonic reactions by phosphorylation, transferring a phosphate group to some other molecule, such as a reactant The recipient molecule is now phosphorylated Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

9 Fig Membrane protein P Solute P i Solute transported ATP (a) Transport work: ATP phosphorylates transport proteins ADP + Vesicle Cytoskeletal track P i ATP Motor protein Protein moved (b) Mechanical work: ATP binds noncovalently to motor proteins, then is hydrolyzed

10 The Regeneration of ATP ATP is a renewable resource that is regenerated by addition of a phosphate group to adenosine diphosphate (ADP) The energy to phosphorylate ADP comes from catabolic reactions in the cell The chemical potential energy temporarily stored in ATP drives most cellular work Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

11 Fig ATP + H 2 O Energy from catabolism (exergonic, energy-releasing processes) ADP + P i Energy for cellular work (endergonic, energy-consuming processes)

12 Concept 8.4: Enzymes speed up metabolic reactions by lowering energy barriers A catalyst is a chemical agent that speeds up a reaction without being consumed by the reaction An enzyme is a catalytic protein Hydrolysis of sucrose by the enzyme sucrase is an example of an enzyme-catalyzed reaction Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

13 Fig Sucrose (C 12 H 22 O 11 ) Sucrase Glucose (C 6 H 12 O 6 ) Fructose (C 6 H 12 O 6 )

14 The Activation Energy Barrier Every chemical reaction between molecules involves bond breaking and bond forming The initial energy needed to start a chemical reaction is called the free energy of activation, or activation energy (E A ) Activation energy is often supplied in the form of heat from the surroundings Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

15 Fig A B C D Transition state A C B D E A Reactants A C B D G < O Products Progress of the reaction

16 How Enzymes Lower the E A Barrier Enzymes catalyze reactions by lowering the E A barrier Enzymes do not affect the change in free energy ( G); instead, they hasten reactions that would occur eventually Animation: How Enzymes Work Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

17 Fig Course of reaction without enzyme Reactants Course of reaction with enzyme E A without enzyme E A with enzyme is lower G is unaffected by enzyme Progress of the reaction Products

18 Substrate Specificity of Enzymes The reactant that an enzyme acts on is called the enzyme s substrate The enzyme binds to its substrate, forming an enzyme-substrate complex The active site is the region on the enzyme where the substrate binds Induced fit of a substrate brings chemical groups of the active site into positions that enhance their ability to catalyze the reaction Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

19 Fig Substrate Active site Enzyme Enzyme-substrate complex (a) (b)

20 Catalysis in the Enzyme s Active Site In an enzymatic reaction, the substrate binds to the active site of the enzyme The active site can lower an E A barrier by Orienting substrates correctly Straining substrate bonds Providing a favorable microenvironment Covalently bonding to the substrate Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

21 Fig Substrates enter active site; enzyme changes shape such that its active site enfolds the substrates (induced fit). 2 Substrates held in active site by weak interactions, such as hydrogen bonds and ionic bonds. Substrates Enzyme-substrate complex 3 Active site can lower E A and speed up a reaction. 6 Active site is available for two new substrate molecules. Enzyme 5 Products are released. 4 Substrates are converted to products. Products

22 Effects of Local Conditions on Enzyme Activity An enzyme s activity can be affected by General environmental factors, such as temperature and ph Chemicals that specifically influence the enzyme Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

23 Effects of Temperature and ph Each enzyme has an optimal temperature in which it can function Each enzyme has an optimal ph in which it can function Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

24 Rate of reaction Rate of reaction Fig Optimal temperature for typical human enzyme Optimal temperature for enzyme of thermophilic (heat-tolerant) bacteria Temperature (ºC) (a) Optimal temperature for two enzymes Optimal ph for pepsin (stomach enzyme) Optimal ph for trypsin (intestinal enzyme) ph (b) Optimal ph for two enzymes

25 Cofactors Cofactors are nonprotein enzyme helpers Cofactors may be inorganic (such as a metal in ionic form) or organic An organic cofactor is called a coenzyme Coenzymes include vitamins Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

26 Enzyme Inhibitors Competitive inhibitors bind to the active site of an enzyme, competing with the substrate Noncompetitive inhibitors bind to another part of an enzyme, causing the enzyme to change shape and making the active site less effective Examples of inhibitors include toxins, poisons, pesticides, and antibiotics Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

27 Fig Substrate Active site Competitive inhibitor Enzyme (a) Normal binding (b) Competitive inhibition Noncompetitive inhibitor (c) Noncompetitive inhibition

28 Concept 8.5: Regulation of enzyme activity helps control metabolism Chemical chaos would result if a cell s metabolic pathways were not tightly regulated A cell does this by switching on or off the genes that encode specific enzymes or by regulating the activity of enzymes Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

29 Allosteric Regulation of Enzymes Allosteric regulation may either inhibit or stimulate an enzyme s activity Allosteric regulation occurs when a regulatory molecule binds to a protein at one site and affects the protein s function at another site Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

30 Allosteric Activation and Inhibition Most allosterically regulated enzymes are made from polypeptide subunits Each enzyme has active and inactive forms The binding of an activator stabilizes the active form of the enzyme The binding of an inhibitor stabilizes the inactive form of the enzyme Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

31 Fig Allosteric enyzme with four subunits Active site (one of four) Regulatory site (one of four) Active form Activator Stabilized active form Oscillation Nonfunctional active site Inactive form Inhibitor (a) Allosteric activators and inhibitors Substrate Stabilized inactive form Inactive form Stabilized active form (b) Cooperativity: another type of allosteric activation

32 Fig. 8-20a Allosteric enzyme with four subunits Active site (one of four) Regulatory site (one of four) Active form Activator Stabilized active form Oscillation Nonfunctional active site Inactive form Inhibitor Stabilized inactive form (a) Allosteric activators and inhibitors

33 Cooperativity is a form of allosteric regulation that can amplify enzyme activity In cooperativity, binding by a substrate to one active site stabilizes favorable conformational changes at all other subunits Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

34 Fig. 8-20b Substrate Inactive form Stabilized active form (b) Cooperativity: another type of allosteric activation

35 Identification of Allosteric Regulators Allosteric regulators are attractive drug candidates for enzyme regulation Inhibition of proteolytic enzymes called caspases may help management of inappropriate inflammatory responses Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

36 Fig EXPERIMENT Caspase 1 Active site Substrate SH Known active form SH Active form can bind substrate SH Allosteric binding site Allosteric Known inactive form inhibitor S S Hypothesis: allosteric inhibitor locks enzyme in inactive form RESULTS Caspase 1 Active form Inhibitor Allosterically inhibited form Inactive form

37 Fig. 8-21a EXPERIMENT Caspase 1 Active site Substrate SH Known active form SH Active form can bind substrate SH Allosteric binding site Allosteric Known inactive form inhibitor S S Hypothesis: allosteric inhibitor locks enzyme in inactive form

38 Fig. 8-21b RESULTS Caspase 1 Active form Allosterically inhibited form Inhibitor Inactive form

39 Feedback Inhibition In feedback inhibition, the end product of a metabolic pathway shuts down the pathway Feedback inhibition prevents a cell from synthesizing more product than is needed Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

40 Fig Initial substrate (threonine) Isoleucine used up by cell Isoleucine binds to allosteric site Feedback inhibition Active site available Active site of enzyme 1 no longer binds threonine; pathway is switched off. Intermediate A Enzyme 2 Intermediate B Enzyme 3 Intermediate C Enzyme 4 Intermediate D Enzyme 5 Threonine in active site Enzyme 1 (threonine deaminase) End product (isoleucine)

41 Specific Localization of Enzymes Within the Cell Structures within the cell help bring order to metabolic pathways Some enzymes act as structural components of membranes In eukaryotic cells, some enzymes reside in specific organelles; for example, enzymes for cellular respiration are located in mitochondria Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

42 Fig Mitochondria 1 µm

43 Fig. 8-UN2 Course of reaction without enzyme Reactants Course of reaction with enzyme E A without enzyme E A with enzyme is lower G is unaffected by enzyme Progress of the reaction Products

44 Fig. 8-UN3

45 Fig. 8-UN4

46 Fig. 8-UN5

47 You should now be able to: 1. Distinguish between the following pairs of terms: catabolic and anabolic pathways; kinetic and potential energy; open and closed systems; exergonic and endergonic reactions 2. In your own words, explain the second law of thermodynamics and explain why it is not violated by living organisms 3. Explain in general terms how cells obtain the energy to do cellular work Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

48 4. Explain how ATP performs cellular work 5. Explain why an investment of activation energy is necessary to initiate a spontaneous reaction 6. Describe the mechanisms by which enzymes lower activation energy 7. Describe how allosteric regulators may inhibit or stimulate the activity of an enzyme Copyright 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

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