BONE HISTOLOGY SLIDE PRESENTATION

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1 BONE HISTOLOGY SLIDE PRESENTATION PRESENTED BY: SKELETECH, INC. Clients and Friends: SkeleTech invites you to use these complimentary images for your own presentations or as teaching slides for bone biology. We hope that when you share the quality of our efforts that you will take a moment to acknowledge SkeleTech as the creator and distributor of this work. Thank you in advance for this consideration and the many opportunities that we have had to enhance the efforts of bone research groups around the globe. SkeleTech, Inc th Ave SE Suite 104 Bothell, WA _01 Tel / Fax / info@skeletech.com

2 Slide 1. Cortical Osteons (Harversian system) Slide 2. Cortical Osteons (Harversian system) Polarized Light (Magnification: 200x) Bright Field (Magnification: 200x) Slide 1 is an unstained ground section of monkey femur mid-shaft viewed under polarized light. This slide shows mature BRU (bone remodeling units) when cortical bone remodeling is completed. Note osteons with lamellar bone arranged in concentric layers and a central Harversian canal (open arrows). Also note interstitial bone between osteons. (sections highlighted in red) Original magnification 200X. Slide 2. Same bone section (Slide 1) when viewed under bright field. Note osteocytes arranged in concentric layers and in parallel with lamellar bone. Slide 3. Mineralized bone matrix, osteoid &. osteoclasts Slide 4. Mineralized bone matrix, osteoid & osteoclasts Goldner s Trichrome Stained (Magnification: 100x) Goldner s Trichrome Stained (Magnification: 200x) Slide 3. A section of monkey lumbar vertebral body showing bone matrix not yet mineralized, i.e., osteoid (stained red), mineralized bone matrix (stain green). The structure at the arrow is the bone resorbing osteoclasts. Undecalcified, 5-µm Goldner s trichrome stained. Slide 4. Same bone section (Slide 3) at higher magnification. SkeleTech Bone Histology Slide Set Page 2 of 7

3 Slide 5. Cortical Bone Remodeling Slide 6. Cortical Bone Remodeling Toluidine Blue Stained (400x) Slide 5. A section of monkey femoral neck cortical bone showing cortical bone remodeling in progress (solid arrows) and several osteons that have completed a remodeling cycle (open arrows). Note the space created by osteoclastic bone resorption, which is being filled by newly synthesized but not yet mineralized bone matrix (i.e., osteoid, stained light blue) and osteoblasts on the osteoid surface. Undecalcified, 7-µm toluidine blue stained. Slide 6. Same bone section (Slide 5) at higher magnification showing a mature osteon that has completed a remodeling cycle. Slide 7. Cortical Bone Remodeling (different field) Slide 8. Cortical Bone Remodeling (different field) Toluidine Blue Stained (400x) Slide 7. A section of monkey femoral neck cortical bone showing cortical bone remodeling in progress. Undecalcified, 7-µm toluidine blue stained. Slide 8. Same bone section (Slide 7) at higher magnification. The two remodeling sites are in a bone formation phase with osteoid seams (solid arrow) and osteoblasts (open arrows) clearly visible. SkeleTech Bone Histology Slide Set Page 3 of 7

4 Slide 9. Cancellous Osteon. Toluidine Blue Stained (100x) Slide 10. Cancellous Osteon. Slide 9. A section of monkey femoral neck showing a cancellous osteon, which represents the end of a remodeling cycle. Note a packet of lamellar bone deposited on the previous resorbing surface (cement line-solid arrows). A new remodeling site within the cancellous bone is also shown. Undecalcified, 7-µm toluidine blue stained. Slide 10. Same bone section (Slide 9) at higher magnification. Slide 11. Cancellous Bone Remodeling Toluidine Blue Stained (100x) Slide 12. Cancellous Bone Remodeling Slide 11. A section of monkey lumber vertebral body showing active bone remodeling in cancellous bone. Note osteoclastic bone resorption (resorption phase) at the lower left (solid arrows) and new bone formation (formation phase) at the upper right (open arrows). Undecalcified, 5-µm toluidine blue stained. Slide 12. Same bone section (Slide 11) at higher magnification. SkeleTech Bone Histology Slide Set Page 4 of 7

5 Slide 13. Osteoclastic Bone Resorption Slide 14. Cancellous bone formation Goldner s Trichrome Stained (Magnification: 200x) Goldner s Trichrome Stained (Magnification: 200x) Slide 13. A section of monkey lumbar vertebral body showing two cancellous bone osteoclasts (solid arrows) with less defined resorptive cavities and one larger osteoclast with a prominent resorptive cavity (Howship s lacunae- open arrow). Undecalcified, 5-µm Goldner s trichrome stained. Original magnification 200X. Slide 14. A section of monkey lumbar vertebral body showing a layer of closely arranged osteoblasts (solid arrows) and their newly deposited bone on the cancellous bone surface. A new remodeling site within the cancellous bone is also shown (open arrow). Undecalcified, 5-µm Golder s trichrome stained. Original magnification 200X. Slide 15. Longitudinal Section of a Monkey Lumbar Slide 16. Cross Section of a Monkey Lumbar Vertebral Vertebral Body. (Magnification: 10x) Body. (Magnification: 10x) Slide 15. A section of monkey lumbar vertebral body stained with the von Kossa s method plus Macneal s tetrachrome counterstain. Mineralized bone is stained black and soft tissues are stained light blue. (Original magnification 10x) Slide 16 A section of monkey lumbar vertebra body stained with the von Kossa s method to show mineralized cortical bone and cancellous bone (black). (Original magnification 10x) SkeleTech Bone Histology Slide Set Page 5 of 7

6 Slide 17. Calcein labels in cortical bone Slide 18. Calcein labels in cortical bone Calcein Labels (Magnification: 100x) Calcein Labels (Magnification: 200x) Slide 17. A section of monkey femoral midshaft cortical bone showing periosteal and intracortical labels as seen under fluorescence microscopy. Bone marrow is on the right. Unstained 50-µm ground section. Slide 18. Same bone section (Slide 17) at higher magnification to show periosteal single (solid arrow) and double labels (open arrow). Slide 19. Endosteal and intracortical labels Slide 20. Endosteal and intracortical labels Calcein Labels (Magnification: 100x) Calcein Labels (Magnification: 200x) Slide 19. A section of monkey femoral midshaft cortical bone showing endosteal and intracortical bone calcein labels as seen under fluorescence microscopy. Bone marrow is on the upper left. Unstained 50- µm ground section. Original magnification 100X. Slide 20. Same bone section (Slide 24) at higher magnification. Original magnification 200X SkeleTech Bone Histology Slide Set Page 6 of 7

7 Slide 21. Calcein Labels in Cancellous Bone Calcein Labels (Magnification: 200x) Slide 21. A section of monkey lumbar vertebral body showing single (solid arrow) and double labels (open arrow). Unstained 8-µm section. Slide 22. Calcein and Alizarin Red Labels in Cancellous Bone. Calcein and Alizarin Red Labels (Magnification: 100x) Slide 22. A section of monkey femoral neck showing alizarin red labels given first (red, single (yellow solid arrow) and double (yellow open arrow)) followed by calcein labels (yellowish green, single (white solid arrow) and double (white open arrow)) in cancellous bone as seen under fluorescent microscopy. Unstained 10-µm section. SkeleTech Bone Histology Slide Set Page 7 of 7

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