f Hydroxytestosterone sulfate in blank urine [M-H] - : c 109 Hydroxytestosterone in deconjugated urine [M+H] + : [M+H] + : 289.

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1 96 OH f Ⅱ Ⅰ Hydroxytestosterone sulfte in blnk urine [M-H] - : b g Ⅱ Ⅰ Hydroxytestosterone in deconjugted urine [M+H] + : c OH h Authentic testosterone [M+H] + : d e Authentic Authentic testosterone testosterone Supplementry Figure 1. Identifiction nd chrcteriztion of hydroxytestosterone sulfte., b: MS/MS spectrum of urinry hydroxytestosterone sulfte I nd II. c, d: MS/MS spectrum of hydroxytestosterone I nd II in deconjugted urine. e: MS/MS spectrum of uthentic testosterone. f: Chromtogrm of hydroxytestosterone sulfte in blnk urine. g: Chromtogrm of hydroxytestosterone in deconjugted urine. h: Chromtogrm of uthentic testosterone.

2 d GCDCA sulfte in blnk urine [M-H] - : b 74 e GCDCA in deconjugted urine [M-H] - : c Authentic GCDCA f Authentic GCDCA [M-H] - : Supplementry Figure 2. Identifiction nd chrcteriztion of GCDCA sulfte. : MS/MS spectrum of urinry GCDCA sulfte. b: MS/MS spectrum of GCDCA in deconjugted urine. c: MS/MS spectrum of uthentic GCDCA. d: Chromtogrm of GCDCA sulfte in blnk urine. e: Chromtogrm of GCDCA in deconjugted urine. f: Chromtogrm of uthentic GCDCA.

3 Urinry ndrosterone sulfte 96 b Authentic ndrosterone sulfte Supplementry Figure 3. Identifiction nd chrcteriztion of ndrosterone sulfte. : MS/MS spectrum of urinry ndrosterone sulfte. b: MS/MS spectrum of uthentic ndrosterone sulfte.

4 Urinry DHEA sulfte 96 b Authentic DHEA sulfte Supplementry Figure 4. Identifiction nd chrcteriztion of DHEA sulfte. : MS/MS spectrum of urinry DHEA sulfte. b: MS/MS spectrum of uthentic DHEA sulfte.

5 Urinry р-cresol sulfte 107 Supplementry Figure 5. MS/MS spectrum of urinry p-cresol sulfte.

exmined using multiple tests, nd only those steroids tht were significntly

6 Rifmpin Control Supplementry Figure 6. Hierrchiclly clustered het mps of the urinry steroid levels in the control nd rifmpin groups. Differences between the steroid levels in the control nd rifmpin groups were exmined using multiple tests, nd only those steroids tht were significntly different t n FDR of < 0.05 were selected. Rows nd columns represent the urinry concentrtions of individul steroids nd subjects, respectively. Ech cell is colored bsed on the concentrtion present in urine smples.

7 Supplementry Tble 1. List of the GC-MS steroids used in the quntittive nlysis. Compounds (trivil nme) Abbrevition Compounds (trivil nme) Abbrevition Androgens (28) Progestins (9) Dihydrotestosterone DHT Pregnenolone Preg Epidihydrotestosterone Epi-DHT 5β-dihydroprogesterone 5β-DHP Dehydroepindrosterone DHEA 5α-dihydroprogesterone 5α-DHP Testosterone T Pregnnolone P-one Epitestosterone EpiT Allopregnnolone Allo-P-one 5α-ndrostn-3α, 17b-diol ααβ-diol Isopregnnolone Iso-P-one 5α-ndrostn-3β, 17β-diol αββ-diol Pregnnediol P-diol 5β-ndrostn-3α, 17β-diol βαβ-diol Pregnnetriol P-triol 5β-ndrostn-3α, 17α-diol βαα-diol 17α-hydroxypregnenolone 17α-OH-Preg 5α-ndrostn-3α, 17α-diol ααα-diol Androstenedione A-dione Sterols (3) Androstenediol A-diol Cholesterol Chol Androsterone An Desmolesterol Desmolesterol Etiocholnolone Etio 24S-hydroxycholesterol 24S-OH-Chol 11β-hydroxyndrosterone 11β-OH-An 11β-hydroxyetiocholnolone 11β-OH-Etio Corticoids (24) 16α-hydroxyDHEA 16α-OH-DHEA Cortisol F Epindrosterone Epi-An Allodihydrocortisol llo-dhf 5α-Androstnedione 5α-dione Corticosterone B 7α-hydroxyDHEA 7α-OH-DHEA Allodihydrocorticosterone llo-dhb 7β-hydroxyDHEA 7β-OH-DHEA Allotetrhydrocorticosterone llo-thb 7α-hydroxytestosterone 7α-OH-T 11-deoxycortisol 11-deoxyF 4-hydroxyndrostenedione 4-OH-A-dione Cortisone E 11β-hydroxyndrostenedione 11β-OH-A-dione Allotetrhydrocortisol llo-thf 16α-hydroxyndrostenedione 16α-OH-A-dione 11-dehydrocorticosterone 11-dehydroB 6β-hydroxytestosterone 6β-OH-T Tetrhydrodeoxycortisol THS 11β-hydroxytestosterone 11β-OH-T Tetrhydrocortisone THE 16α-hydroxytestosterone 16α-OH-T Tetrhydrocortisol THF Tetrhydrodeoxycorticosterone THDOC Estrogens (11) Tetrhydrocorticosterone THB Estrone E1 Tetrhydro-11-dehydrocorticosterone THA Estrdiol E2 α-cortolone α-cortolone Estriol E3 β-cortolone β-cortolone 2-hydroxyestrone 2-OH-E1 20α-dihydrocortisone 20α-DHE 2-hydroxyestrdiol 2-OH-E2 α-cortol α-cortol 2-hydroxyestriol 4-OH-E2 β-cortol β-cortol 2-methoxyestrone 2-MeO-E1 20α-dihydrocortisol 20α-DHF 2-methoxyestrdiol 2-MeO-E2 Allotetrhydrodeoxycorticosterone llo-thdoc 4-methoxyestrdiol 4-MeO-E2 llotetrhydrocortisone llo-the 16-epiestriol 16-epi-E3 Alltetrhydrodeoxycortisol llo-ths 2-hydroxyestriol 2-OH-E3

Arlt W et al.: Steroid Metabolome analysis reveals prevalent glucocorticoid excess in

Arlt W et al.: Steroid Metabolome analysis reveals prevalent glucocorticoid excess in Figure S1: Comparison of 24-h urinary steroid metabolite excretion in 162 healthy controls and 174 patients with. Panel A, tetrahydroaldosterone; Panel B, cortisol; Panel C, total glucocorticoid metabolites.