Guidelines for workup of Throat and Genital Cultures

Size: px
Start display at page:

Download "Guidelines for workup of Throat and Genital Cultures"

Transcription

1 Guidelines for workup of Throat and Genital Cultures 1

2 Acute Pharyngitis By far the most common infection of the upper respiratory tract Viral infection is by far the most common cause of pharyngitis The most important bacterial pathogen in pharyngitis is Streptococcus pyogenes (GAS) 20-30% children / 5-15% in adults 2

3 Group A Streptococcus Pharyngitis Group A Streptococcus (GAS) is the only common cause of acute pharyngotonsillitis that routinely requires antibiotic treatment Accounts for 15% to 30% of sore throats in children and 5% to 10% in adults Highest rates of infection are in children aged 5 to 11 years 3

4 Streptococcal Pharyngitis Other b-haemolytic streptococci produce symptoms similar to, but more mild than those of GAS These other strains are usually NOT associated with rheumatic fever as a noninfectious sequela GAS can occasionally colonize the oropharynx in small numbers, However Groups C & G streptococci are regular colonizers Therefore difficult to determine clinical significance If in pure culture or as a predominating organism, might be reasonable to report their presence 4

5 Pharyngitis (cont d) Arcanobacterium haemolyticum Formerly known as Corynebacterium haemolyticum (small GPB), cat -, b hem. Rarely causes pharyngitis Symptoms closely mimics GAS pharyngitis Exudative pharyngitis Lymphadenopathy Scarlatiniform rash frequent (scarlet fever?) Primarily affects teenagers and young adults year old groups 5

6 6

7 Pharyngitis (cont d) Arcanobacterium haemolyticum Reverse CAMP positive The b-hemolysin of S.aureus on blood agar is inhibited by the phospholipase of A.haemolyticum Appears as an arrow-head-shaped nonhemolyzed area between S.aureus and A.haemolyticum streaked at right angles to each other Additional ID tests: LAP + 7

8 Reverse CAMP CAMP 8

9 Throat cultures: So what to do? 9

10 Throat Culture Inspect blood plates for b- hemolytic colonies after overnight incubation No b colonies b colonies Colony count of b- hemolytic colonies b colonies Reincubate plates overnight No b colonies Report as negative for Group A Strep Perform Streptex A latex agglutination test Negative Are b colonies greater than oral flora? Yes Perform smear and cat Positive No Perform Cat (=) and smear (gpc) Report as negative for Group A Strep Smear not gpb or gpc OR organism is cat + gpb cat = gpc cat = Report as positive for Group A Strep Perform reverse camp test Report b streptococcus, not group A Negative Positive Report as negative for Group A Strep Arcanobacterium hemolyticum 10

11 11

12 12

13 13

14 MALDI 14

15 Throat cultures: Summary Report any amount of GAS If > oral flora, report presence of: Arcanobacterium haemolyticum b-streptococci, not GAS (may group/report C or G) Do not report: Neisseria spp Haemophilus spp. S.pneumoniae S.aureus No indication that growing these in tht cultures means anything in pharyngitis. 15

16 Rapid GAS antigen detection - appropriate utilization Children/Adolescents negative RADT results should be confirmed with a throat culture result Adults low incidence of streptococcal infection extremely low risk of rheumatic fever negative RADT results do not need culture confirmation Clin Infect Dis. 2002;35(2): doi: /

17 GAS Pharyngitis - Diagnosis Rapid testing & when to back up with culture Shulman et al, CID Clinical practice guideline for the diagnosis and management of group A streptococcal pharyngitis: 2012 update by the IDSA. In children and adolescents, negative RADT tests should be backed up by a throat culture. In adults, negative RADT tests do not need to be backed up by a throat culture. The risk of acute rheumatic fever is exceptionally low in adults Abbott et al, CID Reflexive Culture in Adolescents and Adults With Group A Streptococcal Pharyngitis. Culturing samples from adults with negative rapid antigen detection tests identified additional individuals who can benefit from treatment Felsenstein et al, JCM Molecular and Clinical Diagnosis of Group A Streptococcal Pharyngitis in Children. (illumigene assay) Rapid molecular assays are far superior to a RADT and culture for the detection of GAS, when testing is limited to patients presenting with clinical symptoms. 17

18 Molecular detection for GAS (POC) (culture back up necessary?) 3 rd Q

19 DISCUSSION 19

20 Genital cultures What is Vaginitis? By definition, vaginitis refers to vaginal irritation and discharge Causes of vaginitis 3 agents cause > 90% of cases Cervicitis Trichomonas Yeast Bacterial vaginosis (BV) Neisseria gonorrhoeae molecular Chlamydia trachomatis molecular Herpes Simplex virus culture or molecular Cytomegalovirus culture or molecular Wet prep Antigen detection NAAT There really is no place for a routine vaginal culture. 20

21 Vaginal cultures OK That being said We are all pressured to do routine vaginal cultures There is no clinical studies to show that other organisms cause vaginitis So, what should we do? 21

22 Vaginal cultures Look for growth of any organism in quantities > the amount of normal vaginal flora present in the culture. Note: Gardnerella spp. is considered normal vaginal flora. Quantitate and report the amount of normal vaginal flora (VF) present. If there is growth of the following in quantities > VF: Yeast quantitate and report yeast GBS (β or γ) quantitate and report Group B streptococci Enterics quantitate and report enteric gram negative bacilli If there is overgrowth of any other organism consult Do not perform susceptibilities on any organism. Consult all cultures on patients < 15 y.o. 22

23 DISCUSSION 23

Upper Respiratory Infections. Mehreen Arshad, MD Assistant Professor Pediatric Infectious Diseases Duke University

Upper Respiratory Infections. Mehreen Arshad, MD Assistant Professor Pediatric Infectious Diseases Duke University Upper Respiratory Infections Mehreen Arshad, MD Assistant Professor Pediatric Infectious Diseases Duke University Disclosures None Objectives Know the common age- and season-specific causes of pharyngitis

More information

EDUCATIONAL COMMENTARY THROAT CULTURES LEARNING OUTCOMES. Upon completion of this exercise, the participant should be able to:

EDUCATIONAL COMMENTARY THROAT CULTURES LEARNING OUTCOMES. Upon completion of this exercise, the participant should be able to: EDUCATIONAL COMMENTARY THROAT CULTURES LEARNING OUTCOMES Upon completion of this exercise, the participant should be able to: distinguish three types of hemolysis produced by bacterial colonies. discuss

More information

This patient had acute pharyngitis, the painful inflammation of the pharynx and surrounding lymphoid tissues.

This patient had acute pharyngitis, the painful inflammation of the pharynx and surrounding lymphoid tissues. CASE ONE 1.1. PATIENT HISTORY Boy with Acute Pharyngitis The patient was a 6 year-old male who had been in good health with no significant medical problems. In late September he presented to his pediatrician

More information

Culture of Throat, Sputum and Other Respiratory Specimens

Culture of Throat, Sputum and Other Respiratory Specimens Culture of Throat, Sputum and Other Respiratory Specimens Carol Spiegel, Ph.D., D(ABMM) Professor of Pathology and Laboratory Medicine at the UW School of Medicine and Public Health and Director of Clinical

More information

Policy # MI_GEN Department of Microbiology. Page Quality Manual TABLE OF CONTENTS INTRODUCTION... 3 LOWER GENITAL TRACT... 5

Policy # MI_GEN Department of Microbiology. Page Quality Manual TABLE OF CONTENTS INTRODUCTION... 3 LOWER GENITAL TRACT... 5 Quality Manual Version: 1.0 CURRENT 1 of 39 Prepared by QA Committee Issued by: Laboratory Manager Revision Date: 8/15/2017 Approved by Laboratory Director: Annual Review Date: 5/1/2018 Microbiologist-in-Chief

More information

II- Streptococci. Practical 3. Objective: Required materials: Classification of Streptococci: Streptococci can be classified according to:

II- Streptococci. Practical 3. Objective: Required materials: Classification of Streptococci: Streptococci can be classified according to: Practical 3 II- Streptococci Objective: 1. Use of blood agar to differentiate between,, and hemolytic streptococci. 2. To know Gram reaction, shape and arrangement of streptococci. 3. To differentiate

More information

Bacteria causing respiratory tract infections

Bacteria causing respiratory tract infections Editing file Bacteria causing respiratory tract infections Objectives : Recognize signs & symptoms of different bacterial respiratory tract infections Be able to come up with a short differential to relevant

More information

Practice Guidelines for the Diagnosis and Management of Group A Streptococcal Pharyngitis

Practice Guidelines for the Diagnosis and Management of Group A Streptococcal Pharyngitis IDSA GUIDELINES Practice Guidelines for the Diagnosis and Management of Group A Streptococcal Pharyngitis Alan L. Bisno, 1 Michael A. Gerber, 2 Jack M. Gwaltney, Jr., 3 Edward L. Kaplan, 5 and Richard

More information

Streptococcus (gram positive coccus)

Streptococcus (gram positive coccus) #13 made by : aseel al-waked corrected by Shatha Khtoum date : 6/11/2016 Streptococcus (gram positive coccus) Slide 2 (56:00): Streptococci Facultative anaerobe Gram-positive usually chains (sometimes

More information

Streptococcus(gram positive coccus) Dr. Hala Al Daghistani

Streptococcus(gram positive coccus) Dr. Hala Al Daghistani Streptococcus(gram positive coccus) Dr. Hala Al Daghistani Streptococci Facultative anaerobe Gram-positive usually chains (sometimes pairs) Catalase negative Non motile Hemolysins Lancefield Groups (C-carbohydrate

More information

Diagnosing Group A Strep pharyngitis - Which Technique is Best for You?

Diagnosing Group A Strep pharyngitis - Which Technique is Best for You? Diagnosing Group A Strep pharyngitis - Which Technique is Best for You? Gregory J. Berry, Ph.D., D(ABMM) Assistant Professor, Pathology and Laboratory Medicine Zucker School of Medicine at Hofstra/Northwell

More information

Advances in STI diagnostics. Dr Paddy Horner Consultant Senior Lecturer University of Bristol

Advances in STI diagnostics. Dr Paddy Horner Consultant Senior Lecturer University of Bristol Advances in STI diagnostics Dr Paddy Horner Consultant Senior Lecturer University of Bristol Advances in STI diagnostics Rapid expansion in on-line STI testing Outstripping NHS expert advice Increasing

More information

Appropriate Use of Antibiotics for the Treatment of Acute Upper Respiratory Tract Infections in Adults

Appropriate Use of Antibiotics for the Treatment of Acute Upper Respiratory Tract Infections in Adults Appropriate Use of Antibiotics for the Treatment of Acute Upper Respiratory Tract Infections in Adults Kyong Ran Peck, M.D. Division of Infectious Diseases Sungkyunkwan University School of Medicine, Samsung

More information

Objectives, Upon completion of this lecture, the student will:

Objectives, Upon completion of this lecture, the student will: Lec.2 Dr.Sarmad Zeiny 2013-2014 BCM Genus Streptococci Objectives, Upon completion of this lecture, the student will: Outline the medically important streptococci species. Classification of genus streptococci.

More information

INTRODUCTION TO UPPER RESPIRATORY TRACT DISEASES

INTRODUCTION TO UPPER RESPIRATORY TRACT DISEASES Upper Respiratory Tract Infections Return to Syllabus INTRODUCTION TO UPPER RESPIRATORY TRACT DISEASES General Goal: To know the major mechanisms of defense in the URT, the major mechanisms invaders use

More information

Streptococcus pyogenes

Streptococcus pyogenes Streptococcus pyogenes From Wikipedia, the free encyclopedia Streptococcus pyogenes S. pyogenes bacteria at 900x magnification. Scientific classification Kingdom: Eubacteria Phylum: Firmicutes Class: Cocci

More information

Upper Respiratory Tract Infections / 42

Upper Respiratory Tract Infections / 42 Upper Respiratory Tract Infections 1 Upper Respiratory Tract Infections Acute tonsillitispharyngitis Acute otitis media Acute sinusitis Common cold Acute laryngitis Otitis externa Mastoiditis Acute apiglottis

More information

Students. Exhibit - Reporting and Exclusion Requirements for Common Communicable Disease. Reporting Mandated to Local Health Authority

Students. Exhibit - Reporting and Exclusion Requirements for Common Communicable Disease. Reporting Mandated to Local Health Authority August 2003 7:280-E2 Students Exhibit - Reporting and Exclusion Requirements for Common Communicable s Animal Bites (potential for rabies) Bacterial Vaginosis Chancroid As soon as possible, but within

More information

Burton's Microbiology for the Health Sciences

Burton's Microbiology for the Health Sciences Burton's Microbiology for the Health Sciences Section VII. Pathogenesis and Host Defense Mechanisms Burton's Microbiology for the Health Sciences Chapter 14. Pathogenesis of Infectious Diseases 1 Chapter

More information

UPPER RESPIRATORY TRACT INFECTIONS. IAP UG Teaching slides

UPPER RESPIRATORY TRACT INFECTIONS. IAP UG Teaching slides UPPER RESPIRATORY TRACT INFECTIONS 1 INTRODUCTION Most common problem in children below 5 years. In this age group they get about 6 8 episodes per year. It includes infections of nasal cavity, throat,

More information

Bacterial infections of the Respiratory Tract 1. By: Nader Alaridah MD, PhD

Bacterial infections of the Respiratory Tract 1. By: Nader Alaridah MD, PhD Bacterial infections of the Respiratory Tract 1 By: Nader Alaridah MD, PhD Introduction The respiratory tract is the most common site of body acquired infection by pathogens and opportunistic pathogens.

More information

Pathogens of the Respiratory System

Pathogens of the Respiratory System Pathogens of the Respiratory System Chapter 21, Pages 531-567 1. Respiratory System Introduction A. Anatomy (Pages 532-534) i. Upper Respiratory Track a. Nasal Hairs b. Paranasal Sinuses c. Nasal Chonchae

More information

Labquality External Quality Assessment Programmes General Bacteriology 1 3/2014

Labquality External Quality Assessment Programmes General Bacteriology 1 3/2014 Labquality External Quality Assessment Programmes General Bacteriology 1 3/2014 Photos and text: Markku Koskela, M.D., Ph.D. Clinical microbiology specialist Nordlab Oulu, Finland Specimen 31/2014 Abscess

More information

Genus Streptococcus General criteria:

Genus Streptococcus General criteria: Genus Streptococcus Mostly commensals but may cause opportunistic infections (S.viridans) - Few are primary pathogens causing wide range of infections and can trigger immunologic disorders (S.pyogenes,

More information

Sexually Transmitted Infections in the Adolescent Population. Abraham Lichtmacher MD FACOG Chief of Women s Services Lovelace Health System

Sexually Transmitted Infections in the Adolescent Population. Abraham Lichtmacher MD FACOG Chief of Women s Services Lovelace Health System Sexually Transmitted Infections in the Adolescent Population Abraham Lichtmacher MD FACOG Chief of Women s Services Lovelace Health System STI in the Adolescent High school students nationwide, 34.2% were

More information

5/1/2017. Sexually Transmitted Diseases Burning Questions

5/1/2017. Sexually Transmitted Diseases Burning Questions Sexually Transmitted Diseases Burning Questions Jeffrey D. Klausner, MD, MPH Professor of Medicine and Public Health University of California Los Angeles Los Angeles, California FORMATTED: 04-03-17 Financial

More information

Office and Laboratory Management of Genital Specimens

Office and Laboratory Management of Genital Specimens Office and Laboratory Management of Genital Specimens Scope This protocol describes how genital specimens should be handled by the clinician and the laboratory once the decision to take a specimen has

More information

I have no disclosures

I have no disclosures Disclosures Streptococcal Pharyngitis: Update and Current Guidelines Richard A. Jacobs, MD, PhD Emeritus Professor of Medicine Division of Infectious Diseases I have no disclosures CID 2012:55;e 86-102

More information

Babak Valizadeh, DCLS

Babak Valizadeh, DCLS Laboratory Diagnosis of Bacterial Infections of the Respiratory Tract Babak Valizadeh, DCLS 1391. 02. 05 2012. 04. 25 Babak_Valizadeh@hotmail.com Biological Safety Cabinet Process specimens in biological

More information

Streptococci and Other Streptococci-like Organisms. By:Dr. Aghaei

Streptococci and Other Streptococci-like Organisms. By:Dr. Aghaei Streptococci and Other Streptococci-like Organisms By:Dr. Aghaei Case Study 9-year-old boy complains of fever and sore throat On examination, his pharynx is red and his tonsils are swollen His cervical

More information

Processing of female genital specimens at Labtests and Northland Pathology Laboratory

Processing of female genital specimens at Labtests and Northland Pathology Laboratory Consultation document Processing of female genital specimens at Labtests and Northland Pathology Laboratory Author: Dr Arlo Upton Page 1 of 9 Date: 07/03/16 Table of contents 1. INTRODUCTION...3 2. CURRENT

More information

The Streptococci. Diverse collection of cocci. Gram-positive Chains or pairs significant pathogens

The Streptococci. Diverse collection of cocci. Gram-positive Chains or pairs significant pathogens The Streptococci Diverse collection of cocci. Gram-positive Chains or pairs significant pathogens Strong fermenters Facultative anaerobes Non-motile Catalase Negative 1 Classification 1 2 Classification

More information

AHS Laboratory Services Central Zone Guide to Lab Services Manual Cultures/Procedures

AHS Laboratory Services Central Zone Guide to Lab Services Manual Cultures/Procedures AEROBIC CULTURE Routine culture and routine aerobic culture are synonymous terms. Aerobic culture attempts to identify bacteria which grow in the presence of oxygen and are associated with potential infection

More information

References Required document for Laboratory Accreditation by the College of American Pathologists.

References Required document for Laboratory Accreditation by the College of American Pathologists. Subject Culture: Lower Respiratory Tract Index Number Lab-3375 Section Laboratory Subsection Microbiology Category Departmental Contact Sarah Stoner Last Revised 9/11/2017 References Required document

More information

CDC Laboratory Update

CDC Laboratory Update CDC Laboratory Update Chlamydia and Gonorrhea Laboratory Guidelines Overview of the APHL / CDC STD Steering Committee Laboratory Recommendations for the Detection of Chlamydia trachomatis, Neisseria gonorrhoeae

More information

PHARMACEUTICAL MICROBIOLOGY -1I PHT 313. Dr. Rasheeda Hamid Abdalla Assistant Professor tmail.com

PHARMACEUTICAL MICROBIOLOGY -1I PHT 313. Dr. Rasheeda Hamid Abdalla Assistant Professor  tmail.com PHARMACEUTICAL MICROBIOLOGY -1I PHT 313 Dr. Rasheeda Hamid Abdalla Assistant Professor E-mail rasheedahamed12@ho tmail.com General Characteristics of Streptococci Gram-positive spherical/ovoid cocci arranged

More information

Staphylococci. Gram stain: gram positive cocci arranged in clusters.

Staphylococci. Gram stain: gram positive cocci arranged in clusters. Microbiology lab Respiratory system Third medical year Lab contents: Gram positive bacteria (Staphylococcus and Streptococcus spp), two types of filamentous fungi (Aspergillus and Penicillium spp), and

More information

7/13/2018. NEW TESTS - Please update your EMR catalog with those appropriate to your practice

7/13/2018. NEW TESTS - Please update your EMR catalog with those appropriate to your practice NEW TESTS - Please update your EMR catalog with those appropriate to your practice 6987003 MySwab Vaginosis Profile MySwab Vag Group LOINC Result Code Mnemonic Result Name Reference Range UOM Result Type

More information

RESPIRATORY TRACT INFECTIONS. CLS 212: Medical Microbiology

RESPIRATORY TRACT INFECTIONS. CLS 212: Medical Microbiology RESPIRATORY TRACT INFECTIONS CLS 212: Medical Microbiology Anatomy of the Respiratory System Respiratory Infections Respiratory tract can be divided into: Upper Respiratory Tract (URT): Sinuses Nasopharynx,.

More information

**Florida licensees, please note: This exercise is NOT intended to fulfill your state education requirement for molecular pathology.

**Florida licensees, please note: This exercise is NOT intended to fulfill your state education requirement for molecular pathology. EDUCATIONAL COMMENTARY VAGINAL INFECTIONS Educational commentary is provided through our affiliation with the American Society for Clinical Pathology (ASCP). To obtain FREE CME/CMLE credits click on Earn

More information

ORIGINAL ARTICLE. MICROBIOLOGICAL PROFILE OF VAGINAL SWABS. Sevitha Bhat, Nilica Devi, Shalini Shenoy

ORIGINAL ARTICLE. MICROBIOLOGICAL PROFILE OF VAGINAL SWABS. Sevitha Bhat, Nilica Devi, Shalini Shenoy MICROBIOLOGICAL PROFILE OF VAGINAL SWABS. Sevitha Bhat, Nilica Devi, Shalini Shenoy 1. Assistant Professor, Department of Microbiology, Kasturba Medical College, Mangalore. 2. MSc Student, Department of

More information

Streptococci facultative anaerobe

Streptococci facultative anaerobe THE GENUS STREPTOCOCCUS The genus Streptococcus obtains Gram-positive cocci, nonmotile, nonsporeforming, arranged mostly in chains or in pairs. Most species are facultative anaerobes. Some of streptococci

More information

What's the problem? - click where appropriate.

What's the problem? - click where appropriate. STI Tool v 1.9 @ 16/11/2017 What's the problem? - click where appropriate. Male problems: screening urethral symptoms proctitis in gay men lumps or swellings ulcers or sores skin rash and/or itch Female

More information

Phenoxymethyl penicillin versus co-amoxiclav in the treatment of acute streptococcal pharyngitis, and the role of /Mactamase activity in saliva

Phenoxymethyl penicillin versus co-amoxiclav in the treatment of acute streptococcal pharyngitis, and the role of /Mactamase activity in saliva Journal of Antimicrobial Chemotherapy (1996) 7, 1-18 Phexymethyl penicillin versus co-amoxiclav in the treatment of acute streptococcal pharyngitis, and the role of /Mactamase activity in saliva R. S.

More information

Pyogenic cocci (Staph&Strep) Stijn van der Veen

Pyogenic cocci (Staph&Strep) Stijn van der Veen Pyogenic cocci (Staph&Strep) Stijn van der Veen Pyogenic cocci Spherical-shaped bacteria that are able to cause purulent inflammation of the mucous membranes of serous cavities (abdomen, pleura, pericardium)

More information

Clinical Microbiology CLS 2019 Clinical Microbiology Laboratory Scheme Application Form

Clinical Microbiology CLS 2019 Clinical Microbiology Laboratory Scheme Application Form Laboratory Scheme Application Form complete all sections below and return to LGC Standards Proficiency Testing by email, fax or post. Returning customer Lab ID: Purchase order no.: (compulsory) Round Despatch

More information

Normal Flora PROF. HANAN HABIB DEPARTMENT OF PATHOLOGY COLLEGE OF MEDICINE, KSU

Normal Flora PROF. HANAN HABIB DEPARTMENT OF PATHOLOGY COLLEGE OF MEDICINE, KSU Normal Flora PROF. HANAN HABIB DEPARTMENT OF PATHOLOGY COLLEGE OF MEDICINE, KSU Objectives 1. Define the terms: Normal Flora, Resident flora, Transient flora and carrier state 2. Know the origin of normal

More information

STIs- REVISION. Prof A A Hoosen

STIs- REVISION. Prof A A Hoosen STIs- REVISION Prof A A Hoosen Department of Medical Microbiology, Faculty of Health Sciences, University of Pretoria and the NHLS Microbiology Tertiary Laboratory at the Pretoria Academic Hospital Complex

More information

Clinical Policy Title: Strep testing

Clinical Policy Title: Strep testing Clinical Policy Title: Strep testing Clinical Policy Number: 07.01.09 Effective Date: December 1, 2017 Initial Review Date: October 19, 2017 Most Recent Review Date: November 16, 2017 Next Review Date:

More information

Work up of Respiratory & Wound Cultures:

Work up of Respiratory & Wound Cultures: Work up of Respiratory & Wound Cultures: Culture work up 2 Systematic approaches 1 Work up of Respiratory & Wound Cultures Resident flora Colonizing organisms Pathogens 2 Work up of Respiratory & Wound

More information

Acute Pharyngo-tonsillitis

Acute Pharyngo-tonsillitis Acute Pharyngo-tonsillitis Tania Sih When referring to tonsillitis, the correct designation is pharyngo-tonsillitis (PT) as the pharynx has lateral cords with the same type of tissue as in Waldeyer s lymphatic

More information

IDSA GUIDELINES EXECUTIVE SUMMARY

IDSA GUIDELINES EXECUTIVE SUMMARY IDSA GUIDELINES Clinical Practice Guideline for the Diagnosis and Management of Group A Streptococcal Pharyngitis: 2012 Update by the Infectious Diseases Society of America Stanford T. Shulman, 1 Alan

More information

Medical Bacteriology- Lecture: 6

Medical Bacteriology- Lecture: 6 Medical Bacteriology- Lecture: 6 Gram Positive Cocci Streptococcal Disease Streptococcus pyogenes Classification of Streptococci based on (1- Hemolysis reactions on blood agar) (Brown in 1903) The type

More information

MINIREVIEW. Role of the Microbiology Laboratory in Diagnosis and Management of Pharyngitis. Paul P. Bourbeau*

MINIREVIEW. Role of the Microbiology Laboratory in Diagnosis and Management of Pharyngitis. Paul P. Bourbeau* JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 2003, p. 3467 3472 Vol. 41, No. 8 0095-1137/03/$08.00 0 DOI: 10.1128/JCM.41.8.3467 3472.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved.

More information

Current Issues in Pharyngitis: Carlos A. Arango, M.D., F.A.A.P. Assistant Professor Department of Pediatrics University of Florida

Current Issues in Pharyngitis: Carlos A. Arango, M.D., F.A.A.P. Assistant Professor Department of Pediatrics University of Florida Current Issues in Pharyngitis: Carlos A. Arango, M.D., F.A.A.P. Assistant Professor Department of Pediatrics University of Florida Pharyngitis Inflammation of any structures of the pharynx Common cause

More information

Chapter 14-15, all tables and figures taken from this chapter

Chapter 14-15, all tables and figures taken from this chapter Levinson, W., Review of medical microbiology and immunology. Fourteenth edition. ed. 2016, New York: McGraw-Hill Education. ix, 821 pages. Chapter 14-15, all tables and figures taken from this chapter

More information

Respiratory tract infections. Krzysztof Buczkowski

Respiratory tract infections. Krzysztof Buczkowski Respiratory tract infections Krzysztof Buczkowski Etiology Viruses Rhinoviruses Adenoviruses Coronaviruses Influenza and Parainfluenza Viruses Respiratory Syncitial Viruses Enteroviruses Etiology Bacteria

More information

INFECTIOUS DISEASES IN THE LONG TERM CARE FACILITY

INFECTIOUS DISEASES IN THE LONG TERM CARE FACILITY INFECTIOUS DISEASES IN THE LONG TERM CARE FACILITY The following is a list of the most common infectious diseases that are to be found in the long term care facility. Precautions are recommended and the

More information

OAML Guideline on the Investigation of Genital Tract Infections November, 2015

OAML Guideline on the Investigation of Genital Tract Infections November, 2015 OAML Guideline on the Investigation of Genital Tract Infections November, 2015 1. Purpose The purpose of this guideline is to provide ordering clinicians with a clear and concise reference for the investigation

More information

Comparisons Between Direct Microscopic and Cultural Methods for Recognition of Corynebacterium vaginale in Women with Vaginitis

Comparisons Between Direct Microscopic and Cultural Methods for Recognition of Corynebacterium vaginale in Women with Vaginitis JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1977, p. 268-272 Copyright 1977 American Society for Microbiology Vol. 5, No. 3 Printed in U.S.A. Comparisons Between Direct Microscopic and Cultural Methods for

More information

Who's There? Changing concepts of vaginal microbiota

Who's There? Changing concepts of vaginal microbiota Who's There? Changing concepts of vaginal microbiota Healthy vaginal ecosystem: H 2 O 2 -producing lactobacilli E.J. Baron, Ph.D., D(ABMM), F(AAM), F(IDSA) Prof. Emerita Pathology, Stanford University

More information

Strep-a-Test Twister Test

Strep-a-Test Twister Test Strep-a-Test Twister Test Code: 24524 A rapid test for the qualitative detection of Strep A antigen in throat swab specimens. For professional in vitro diagnostic use only. INTENDED USE The Strep A Twist

More information

A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci

A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci J. clin. Path. (1964), 17, 231 A new selective blood agar medium for Streptococcus pyogenes and other haemolytic streptococci E. J. L. LOWBURY, A. KIDSON, AND H. A. LILLY From the Medical Research Council

More information

Microbiology - Problem Drill 22: Microbial Infections of Urinary & Reproductive Systems

Microbiology - Problem Drill 22: Microbial Infections of Urinary & Reproductive Systems Microbiology - Problem Drill 22: Microbial Infections of Urinary & Reproductive Systems No. 1 of 10 1. What is the name of the functional unit of the kidney? (A) Collecting duct (B) Henel s Loop (C) Glomerulus

More information

Sexually Transmi/ed Diseases

Sexually Transmi/ed Diseases Sexually Transmi/ed Diseases Chapter Fourteen 2013 McGraw-Hill Higher Education. All rights reserved. Also known as sexually transmitted infections The Major STDs (STIs) HIV/AIDS Chlamydia Gonorrhea Human

More information

Sexually Transmitted. Dr. Tetty Aman Nasution, MMedSc Departemen Mikrobiologi FK USU Medan

Sexually Transmitted. Dr. Tetty Aman Nasution, MMedSc Departemen Mikrobiologi FK USU Medan Sexually Transmitted Infection Dr. Tetty Aman Nasution, MMedSc Departemen Mikrobiologi FK USU Medan Terminology STI (Sexually Transmitted Infection) : refers to the way of transmission RTI (Reproductive

More information

Follow this and additional works at:

Follow this and additional works at: Bond University epublications@bond Faculty of Health Sciences & Medicine Publications Faculty of Health Sciences & Medicine 5-1-2004 Chris Del Mar Bond University, chris_del_mar@bond.edu.au Paul Glasziou

More information

Online Ordering Available. GBS Detect

Online Ordering Available. GBS Detect Online Ordering Available GBS Detect Fast New color change method for cultivation and identification of beta-hemolytic strains of Group B Streptococcus! Strep B Carrot Broth kit is a color change method

More information

Noorbakhsh S 1*, Tabatabaei A 1, Farhadi M 2, Ebrahimi Taj F 1

Noorbakhsh S 1*, Tabatabaei A 1, Farhadi M 2, Ebrahimi Taj F 1 Volume 3 Number 2 (June 2011) 99-103 Short Communication Immunoasssay chromatographic antigen test for rapid diagnosis of Group A beta hemolytic Streptococcus in children: A cross/ sectional study Noorbakhsh

More information

Group B streptococcal infection;. Bacteremia without a focus occurs in 80-85%,. July has been recognised as Group B Strep Awareness Month,.

Group B streptococcal infection;. Bacteremia without a focus occurs in 80-85%,. July has been recognised as Group B Strep Awareness Month,. Group B streptococcal infection;. Bacteremia without a focus occurs in 80-85%,. July has been recognised as Group B Strep Awareness Month,. 12-10-2017 Group B streptococci are uniformly sensitive to penicillin

More information

Setting The setting was primary care. The economic study was carried out in the USA.

Setting The setting was primary care. The economic study was carried out in the USA. "Shotgun" versus sequential testing: cost-effectiveness of diagnostic strategies for vaginitis Carr P L, Rothberg M B, Friedman R H, Felsenstein D, Pliskin J S Record Status This is a critical abstract

More information

Updated Guidelines for Post-Assault Testing and Treatment

Updated Guidelines for Post-Assault Testing and Treatment Updated Guidelines for Post-Assault Testing and Treatment Ann S. Botash, MD Professor of Pediatrics October 5, 2016!" Disclosure Statement Ann S. Botash, MD, has no financial relationships with any commercial

More information

Foundations in Microbiology

Foundations in Microbiology Foundations in Microbiology Fifth Edition Talaro Chapter 18 Cocci of Medical Importance Chapter 18 2 Cocci of Interest Staphylococcus aureus S. epidermidis, S. hominis, S. capitis S. saprophyticus Streptococcus

More information

GOALS AND OBJECTIVES INFECTIOUS DISEASE

GOALS AND OBJECTIVES INFECTIOUS DISEASE GOALS AND OBJECTIVES INFECTIOUS DISEASE Infectious Disease and HIV Overview: The Infectious Diseases Program at the University of Southern California prepares trainees for the management of problems in

More information

Answers to those burning questions -

Answers to those burning questions - Answers to those burning questions - Ann Avery MD Infectious Diseases Physician-MetroHealth Medical Center Assistant Professor- Case Western Reserve University SOM Medical Director -Cleveland Department

More information

OUTLINE Laboratory Detection and Reporting of Streptococcus agalactiae

OUTLINE Laboratory Detection and Reporting of Streptococcus agalactiae OUTLINE Laboratory Detection and Reporting of Streptococcus agalactiae I. Importance of prenatal screening strategies II. Past approaches Erik Munson Clinical Microbiology Wheaton Franciscan Laboratory

More information

USER GUIDE FOR SPECIMEN COLLECTION

USER GUIDE FOR SPECIMEN COLLECTION DIAGNOSTIC LAB SPECIMEN COLLECTION GUIDE USER GUIDE FOR SPECIMEN COLLECTION ACM GLOBAL LABORATORIES DIAGNOSTIC SERVICES ACM Global Laboratories offers a comprehensive menu of services that support many

More information

Complex Vaginitis Cases: Applying New Diagnostic Methods to Enhance Patient Outcomes ReachMD Page 1 of 5

Complex Vaginitis Cases: Applying New Diagnostic Methods to Enhance Patient Outcomes ReachMD Page 1 of 5 Transcript Details This is a transcript of a continuing medical education (CME) activity accessible on the ReachMD network. Additional media formats for the activity and full activity details (including

More information

How is it transferred?

How is it transferred? STI s What is a STI? It is a contagious infection that is transferred from one person to another through sexual intercourse or other sexually- related behaviors. How is it transferred? The organisms live

More information

Chapter 19. Pathogenic Gram-Positive Bacteria. Staphylococcus & Streptococcus

Chapter 19. Pathogenic Gram-Positive Bacteria. Staphylococcus & Streptococcus Chapter 19 Pathogenic Gram-Positive Bacteria Staphylococcus & Streptococcus Staphylococcus Normal members of every human's microbiota Can be opportunistic pathogens Facultative anaerobes Cells occur in

More information

Index. Infect Dis Clin N Am 21 (2007) Note: Page numbers of article titles are in boldface type.

Index. Infect Dis Clin N Am 21 (2007) Note: Page numbers of article titles are in boldface type. Infect Dis Clin N Am 21 (2007) 591 599 Index Note: Page numbers of article titles are in boldface type. A Abscess(es) epidural, subdural empyema and, 584 586 periotonsillar, microbiologic investigations

More information

Laboratory Detection and Reporting of Streptococcus agalactiae

Laboratory Detection and Reporting of Streptococcus agalactiae Laboratory Detection and Reporting of Streptococcus agalactiae Erik Munson Clinical Microbiology Wheaton Franciscan Laboratory Milwaukee, Wisconsin The presenter states no conflict of interest and has

More information

FINAL. Etiologic Agents & Related Information to Know. Chapter 21: Microbial Diseases of Skin and Eyes Page No. Know term "diphtheroids"...

FINAL. Etiologic Agents & Related Information to Know. Chapter 21: Microbial Diseases of Skin and Eyes Page No. Know term diphtheroids... 10 th Edition Tortora FINAL Microbiology Etiologic Agents & Related Information to Know Chapter 21: Microbial Diseases of Skin and Eyes Know term "diphtheroids"................. 586 Agents of boils and

More information

Bacterial Mechanisms of Pathogenicity. 2 nd Lecture

Bacterial Mechanisms of Pathogenicity. 2 nd Lecture Bacterial Mechanisms of Pathogenicity 2 nd Lecture Preferred Portal of Entry Just because a pathogen enters your body it does not mean it s going to cause disease. pathogens - preferred portal of entry

More information

Upper respiratory tract infections

Upper respiratory tract infections Upper respiratory tract infections Pharyngitis Pharyngitis is an acute infection of the oropharynx or nasopharynx 60-70% pharyngitis is viral Streptococcus sore throat(phyringitis) Streptococcus pyogenes

More information

Medical Bacteriology- Lecture 6

Medical Bacteriology- Lecture 6 Medical Bacteriology- Lecture 6 Streptococci 1 Classification of Streptococci based on (1) - Hemolysis reactions on blood agar) (Brown in 1903) The type of hemolytic reaction on blood agar has long been

More information

1. Which of the following statements concerning Plasmodium vivax is TRUE?

1. Which of the following statements concerning Plasmodium vivax is TRUE? 1 Microm 301 Final Exam 2012 Practice Questions and Key 1. Which of the following statements concerning Plasmodium vivax is TRUE? A. It infects all stages of erythrocytes (immature, mature, and senescent).

More information

Sexually Transmitted Infections. Kim Dawson October 2010

Sexually Transmitted Infections. Kim Dawson October 2010 Sexually Transmitted Infections Kim Dawson October 2010 Objectives: You will learn about: Sexually Transmitted Infections (STI s). How they are transferred. High risk behavior. The most common STI s. How

More information

Rheumatic Fever And Post-streptococcal Reactive Arthritis

Rheumatic Fever And Post-streptococcal Reactive Arthritis www.printo.it/pediatric-rheumatology/gb/intro Rheumatic Fever And Post-streptococcal Reactive Arthritis Version of 2016 1. WHAT IS RHEUMATIC FEVER 1.1 What is it? Rheumatic fever is a disease caused by

More information

Sexual health: Hot topics. Anne Connolly Vice President FSRH

Sexual health: Hot topics. Anne Connolly Vice President FSRH Sexual health: Hot topics Anne Connolly Vice President FSRH To cover Contraception choices Contraception safety Sexual history taking Relevant tests We don t do great. 30 50% pregnancies are unplanned

More information

CLINICAL MICROBIOLOGY AND IMMUNOLOGY. Microbial Mechanisms of Pathogenicity

CLINICAL MICROBIOLOGY AND IMMUNOLOGY. Microbial Mechanisms of Pathogenicity CLINICAL MICROBIOLOGY AND IMMUNOLOGY Microbial Mechanisms of Pathogenicity -Pathogenicity - ability of pathogen to cause disease by overcoming the defenses of the host -Virulence - degree of pathogenicity

More information

Non-reproductive tissues and cells

Non-reproductive tissues and cells Colour key Minimum requirements as set out in Directive 2004/23/EC More stringent - legy binding on national level More stringent - recommended on national level Not legy binding and not recommended on

More information

Professor Jonathan Ross

Professor Jonathan Ross SECOND JOINT CONFERENCE OF BHIVA AND BASHH 2010 Professor Jonathan Ross Whittall Street Clinic, Birmingham COMPETING INTEREST OF FINANCIAL VALUE > 1,000: Speaker Name Statement Professor Ross has received

More information

Clarithromycin versus penicillin in the treatment of streptococcal pharyngitis

Clarithromycin versus penicillin in the treatment of streptococcal pharyngitis Journal of Antimicrobial Chemotherapy (1991) 27, Suppl. A, 67-74 Clarithromycin versus penicillin in the treatment of streptococcal pharyngitis Joseph H. Levenstein* South Africa Academy of Family Practice,

More information

Work-up of Respiratory Specimens Now you can breathe easier

Work-up of Respiratory Specimens Now you can breathe easier 34 th Annual Meeting Southwestern Association of Clinical Microbiology Work-up of Respiratory Specimens Now you can breathe easier Yvette S. McCarter, PhD, D(ABMM) Director, Clinical Microbiology Laboratory

More information

Role of Non-Group A Streptococci in Acute Pharyngitis

Role of Non-Group A Streptococci in Acute Pharyngitis Role of Non-Group A Streptococci in Acute Pharyngitis Jeffrey Tiemstra, MD, and Rosita L. F. Miranda, MD, MS, DLO Background: The role of non-group A streptococci (non-gas) as pathogens of acute pharyngitis

More information

Investigation and Management of Vaginal Discharge in Adult Women

Investigation and Management of Vaginal Discharge in Adult Women Investigation and Management of Vaginal Discharge in Adult Women SUMMARY POINTS A detailed history, including sexual history, should be taken to explore potential causes and guide investigation and management.

More information

Elements for a Public Summary

Elements for a Public Summary VI.2 VI.2.1 Elements for a Public Summary Overview of disease epidemiology Rapenin (phenoxymethylpenicillin potassium) is indicated for the treatment of infections caused by penicillin-sensitive bacteria.

More information

RESPIRATORY TRACT INFECTIONS. CLS 212: Medical Microbiology Zeina Alkudmani

RESPIRATORY TRACT INFECTIONS. CLS 212: Medical Microbiology Zeina Alkudmani RESPIRATORY TRACT INFECTIONS CLS 212: Medical Microbiology Zeina Alkudmani Lower Respiratory Tract Upper Respiratory Tract Anatomy of the Respiratory System Nasopharynx Oropharynx Respiratory Tract Infections

More information