: Instructor of biochemistry at Faculty of science, Ain Shams University, Egypt.
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1 RANIA HASSAN MOHAMED HASSAN, Ph.D. Lecturer of Biochemistry (Work) Ain Shams University, Faculty of Science Department of Biochemistry 11566, Alkalifa Alma'mon St., Alabbasia, Cairo, Egypt. Telephone: Nationality: Egyptian Date of Birth: March 24,1983 Google scholar h index: 2 Education : Ph.D. in Molecular Immunology at Department of Pathology, Graduate School of Medicine, Hokkaido University, Japan : M.Sc. in Molecular Biology at The Unit of the Genetic Engineering and Molecular Biology Research, Department of Biochemistry, Faculty of Science, Ain Shams University, Egypt : B.Sc in Biochemistry (Grade: Excellent with honor) at Department of Biochemistry, Faculty of Science, Ain Shams University, Egypt Academic Jobs: Now: Lecturer at Department of Biochemistry, Faculty of Science, Ain Shams University, Egypt : Research assistant at Department of Pathology, Graduate School of Medicine, Hokkaido University, Japan : Assistant lecturer at Department of Biochemistry, Faculty of Science, Ain Shams University for undergraduate biochemistry students and postgraduate molecular biology diploma students : Instructor of biochemistry at Faculty of science, Ain Shams University, Egypt. Fellowships: : Grant from the Ministry of Higher Education and Scientific Research in Egypt for Ph.D. study in Hokkaido University, Japan : Scholarship from the Academy of Scientific Research and Technology, Egypt for Master study in Ain Shams University, Egypt.
2 Research Experience: Postdoctoral work: Currently: I am 1- Studying mirna replacement therapy delivery by nanoparticles against liver cancer cell stemness. 2- Testing the in vitro and in vivo Graviola extract anticancer effect on hepatocellular carcinoma and on the chemoresistant cancerous spheroid 3D model. Doctoral program: 1) Investigating the role of H60c as an NKG2D ligand expressed on mouse skin keratinocytes and identified at Department of Pathology by Prof. Dr. Kasahara team in the innate immunological response of a unique γδ T cells, dendritic epidermal T cells (DETC), for murine skin wound healing. 2) Evolutionary study in mammals and specially in primates about Skint1, an indispensable protein in selection of DETC in rodents, and the functional correlation between SKINT1L protein and Skin DETC-like cells homing and development in Old World Monkeys. Master program: Master dissertation entitled Modulation of immune response to Schistosoma mansoni through generation of DNA vaccines against selected antigens. These antigens include Calreticulin, Myosin and Calpain which are isolated and/or identified at The Unit of the Genetic Engineering and Molecular biology research by Prof. Dr. Amr Karim. We evaluated the humoral immune response and protection raised by those vaccines in a prime/boost vaccination manner using DNA and recombinant antigens vaccines. Research Technical Skills: During my study I got experience in many relevant techniques including the: 1-Molecular Biology Techniques: -Conventional and real time PCR, cloning, sequencing and sequencing data analysis, generation and purification of recombinant proteins. 2- Molecular Immunology Techniques: - Generation of monoclonal antibodies, vaccination, skin cell isolation, Western blotting, ELISA, immunohistochemistry, cytospin staining, multicolor flow cytometry analysis of immune cells, magnetic cell sorting, cytotoxicity and blocking assays and immunofluorescence microscopy. - In vitro and in vivo animal experimental experience. Efficient management of mouse colonies and dealing with the mouse models, In addition to collection and analysis of the biological samples. - Maintaining mammalian and bacterial cell cultures. 3- Bioinformatics Analysis: -Using different database tools including those found in NCBI, Ensembl, EMBL and several bioinformatics software dealing with gene, transcript, mirna and protein.
3 Research Interest: My previous and current study drives me strongly to be interested in the different innate and adaptive immunological responses against different challenges and the anticancer approaches. Moreover, I am concerned with the genetic basis controlling these responses and diseases. Publications, Presentations at academic seminars, and Awards: 1.Publications: 1) Rania Hassan Mohamed, Yoichi Sutoh, Yasushi Itoh, Noriyuki Otsuka, Yukiko Miyatake, Kazumasa Ogasawara and Masanori Kasahara. The SKINT1-like gene is inactivated in hominoids but not in all primate species: Implications for the origin of dendritic epidermal T cells. PLoS One 10 (4) (2015). 2) Shigeru Yoshida, Rania Hassan Mohamed, Mizuho Kajikawa, Jun Koizumi, Minami Tanaka, Kazunori Fugo, Noriyuki Otsuka, Katsumi Maenaka, Hideo Yagita, Hitoshi Chiba and Masanori Kasahara. Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair. J. Immunol 188, (2012). 2.Presentations at academic seminars: 1- γδ T Cells as an Immunotherapy Approach. The 13 th National Committee for Biochemistry and Molecular Biology Conference. March 23-24, 2016, Cairo, Egypt. 2- The SKINT1-like gene is inactivated in hominoids but not in Old World monkeys. The 23 rd Annual Meeting of the Japanese Society for Histocompitability and Immunogenetics. September 13-15, 2014, Nagasaki, Japan. 3- Inactivation of SKINT1L in hominoids but not in Old World monkeys. The 2013 Annual Meeting of the Japanese Society of Immunology. December 11-13, 2013, Chiba, Japan. 4- Inactivation of Skint1 in hominoids but not in Old World monkeys. The 32 nd Annual Meeting of the Japanese association for research on the Thymus. February 9, 2013, Sapporo, Japan. 5- Blockade of interactions between NKG2D and an NKG2D ligand H60c delays wound healing. The 2012 Annual Meeting of the Japanese Society of Immunology. December 5-7, 2012, Kobe, Japan. 6- Blockade of interactions between NKG2D and an NKG2D ligand H60c delays wound healing. The 91 st Hokkaido Medical Congress of Pathology Association. September 10, 2011, Asahikawa, Japan. 3.Awards:
4 The award of the best presentation entitled "γδ T Cells as an Immunotherapy Approach" from the 13 th National Committee for Biochemistry and Molecular Biology Conference, The award of the young scientist of the year 2014 from Dept. of Pathology, Graduate School of Medicine, Hokkaido University. Memberships and Training Workshops: Memberships: 2007-present: Egyptian Society of Biochemistry and Molecular Biology : Japanese Society for Immunology. Training Workshops: 2012: The 4 th HOPE meeting. March 7-11, Tsukuba, Japan. 2009: The 56th scientific congress of Egyptian society of clinical chemistry discussing the laboratory medicine, update clinical applications of genomic medicine, Cairo University, Cairo, Egypt. 2007: Training workshop in human stem cell principles and applications, National Institute of Research, Cairo, Egypt. 2007: The annual scientific congress of the Egyptian society of biochemistry and molecular biology, Ain Shams University, Cairo, Egypt. Personal Skills: Excellent communication skills in Arabic, English and good skills in Japanese. I have the ability to independently manage research project with perfect team work skills acquired through my academic career. I also have very good computer skills with office, bioinformatics software and writing grant proposals. Moreover, I have enthusiasm in working and teaching in international environment and knowing new cultures. Further, I have the ability to set excellent time and project management. References: Kasahara Masanori, M.D.,Ph.D. Prof., Dean and chairman of Department of Pathology, Graduate school of medicine, Hokkaido University, Japan. e.mail address: mkasaha@med.hokudai.ac.jp Tel: Amr Karim, Ph.D. Professor, ex-chairman of Department of Biochemistry, Faculty of Science, Ain Shams University, Egypt. e.mail address: amrkarim3@yahoo.com Tel: Mohamed Ghazy, Ph.D. Professor of Biochemistry and Molecular biology and Senior Research Scientist at
5 Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, USA. Tel: (716)
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