Keywords. Punnett Square forked line. gene allele dominant recessive character trait phenotype genotype
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1 Genetics
2 Core Concepts Mendel s Law of Segregation states that there are two alleles for every gene determining a specific characteristic, and these alleles are segregated into separate gametes during reproduction. When the 2 different alleles occur together in one individual (heterozygote), the dominant allele will be the one that is expressed in the phenotype of the individual. Mendel s Law of Independent Assortment says that 2 or more different genes, if found on separate chromosomes, are determined independently of each other. The Addition Rule is used to combine probabilities, when there are 2 or more ways to arrive at a given outcome. The Multiplication Rule is used to combine probabilities of 2 or more different events that need to occur in combination. Many characteristics have been found to follow patterns of inheritance that are modifications of Mendel s rules.
3 Keywords gene allele dominant recessive character trait phenotype genotype pure breeding hybrid homozygous heterozygous P (parental generation) F 1 (first filial generation) F 2 (second filial generation) Punnett Square forked line
4 Mendel s Discoveries Pre-Mendel beliefs in genetics: blending theory Augustinian monk and science teacher Why Pisum sativum? Pea plants available in many varieties character (heritable feature) trait (character variant) Perfect flowers cross-pollination and selfpollination Short generation time Many offspring CHARACTERS TRAITS
5 MONOHYBRID CROSS inheritance of a single trait P Generation (true-breeding parents) Purple flowers x White flowers Crossfertilization F 1 Generation (hybrids) All plants had purple flowers self-pollination F 2 Generation 705 plants 224 plants
6 Results for other hybrid (F 1 ) characters
7 Blending inheritance Complete dominance
8 Mendelian Inheritance Complete dominance Allele for purple flowers ( P ) Locus for flower-color gene Homologous pair of chromosomes Allele for white flowers ( p ) Alternative versions of genes exist (alleles) Dominant Recessive Organism inherits 2 alleles Dominant is fully expressed Recessive has no visible effect
9 PP or Pp PP Pp pp pp Alternative versions of genes exist (alleles) Dominant Recessive Organism inherits 2 alleles (genotype) Dominant is fully expressed Recessive has no visible effect
10 Other terms in genetics Homozygous identical alleles, true-breeding Heterozygous different alleles Phenotype physical appearance Genotype genetic make-up
11 Mendelian Inheritance Law of Segregation 2 alleles for one character separate and go to different gametes
12 Parental generation Pure-breeding parents are homozygous for different traits for flower color Each parent produces only one type of gamete: purple parent (P), white parent (p) F 1 generation Hybrids are heterozygous for flower color Can produce two kinds of gametes: P or p F 2 generation Due to the law of segregation, eggs and sperm with different kinds of gametes combine to form a specific ratio Genotypic ratio 1 PP : 2 Pp : 1 pp Phenotypic ratio 3 purple : 1 white
13 An organism displays the dominant phenotype. It may be either homozygous or heterozygous for the trait. Problem-solving How can its genotype be determined? Perform a test-cross: Dominant phenotype x recessive phenotype
14 Dihybrid cross Inheritance of two characters Produces four phenotypes in the F 2 generation
15 Dihybrid cross Inheritance of two characters Characters are NOT transmitted as a package (YR and yr only) Genes are packaged into gametes in all possible combinations of alleles (YR, Yr, yr, yr)
16 Law of independent assortment Allele pairs separate independently during the formation of gametes
17 Genetics and Probability Addition Rule combines probabilities for mutually exclusive events Multiplication Rule combines probabilities of 2 or more independent events that need to occur together Monohybrid cross Round seed shape is dominant over wrinkled seeds in pea plants. What percentage of the F2 generation can be expected to: a. Be homozygous dominant for seed shape? b. Be heterozygous for seed shape? c. Have round seeds?
18 Genetics and Probability Addition Rule combines probabilities for mutually exclusive events Multiplication Rule combines probabilities of 2 or more independent events that need to occur together Dihybrid cross Round seed shape is dominant over wrinkled seeds in pea plants. Green pod color is dominant over yellow pods. What percentage of the F2 generation can be expected to: a. Be heterozygous for both characters? b. Have round seeds in green pods? c. Have wrinkled seeds in green pods?
19 Genetics and Probability Addition Rule combines probabilities for mutually exclusive events Multiplication Rule combines probabilities of 2 or more independent events that need to occur together Trihybrid cross Round seed shape is dominant over wrinkled seeds in pea plants. Green pod color is dominant over yellow pods. Tall plants are dominant over short ones. What percentage of the F2 generation can be expected to: a. Be heterozygous for all characters? b. Be tall plants with round seeds in yellow pods? c. Be short plants with round seeds in green pods?
20 Reminders for the Perio 1. Last exam on Thursday (PM sked), after Math 2. Coverage: Cell Cycle Non-Mendelian inheritance; guide questions available online 3. NBs (4% of grade) + any resubmissions due at 2:30 PM on the day of the Bio Perio 4. EoQRs available online due Aug 11 (Sun), 12 NN
21 Find the genotypic and phenotypic ratios of the offspring from the following crosses using the forked line method: 1. AaBb x aabb 2. AABbCc x AaBbcc
22 Non-mendelian inheritance Alleles with different degrees of dominance and recessiveness Codominance - two dominant alleles affect phenotype in separate ways - both alleles manifest - e.g. roan coloring in horses both red and white Incomplete dominance - phenotype of offspring is between phenotypes of two parents - e.g. red and white parents give rise to pink offspring Multiple alleles - genes with more than two alleles that control the phenotype - e.g. ABO blood type system has 3 alleles-- I A,I B,i. I A,I B are codominant, i is recessive to both
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27 Sample problems 1. A cross between a blue blahblah bird & a white blahblah bird produces silver blahblah birds. a) What are the genotypes of the parent blahblah birds in the original cross? b) What is the genotype of the silver offspring? c) What would be the phenotypic ratios of offspring produced by two silver blahblah birds? 2. The color of fruit for plant "X" is determined by two alleles. When two plants with orange fruits are crossed the following phenotypic ratios are present in the offspring: 25% red fruit, 50% orange fruit, 25% yellow fruit. What are the genotypes of the parent orange-fruited plants? 3. Predict the phenotypic ratios of offspring when a homozygous white cow is crossed with a red roan bull. 4. What should the genotypes & phenotypes for parent cattle be if a farmer wanted only cattle with red fur?
28 Sample problems 5. A cross between a black cat & a tan cat produces a tabby pattern (black & tan fur together). a) What pattern of inheritance does this illustrate? b) What percent of kittens would have tan fur if a tabby cat is crossed with a black cat? 6. A woman with Type O blood and a man who is Type AB are expecting a child. What are the possible blood types of the kid? 7. What are the possible blood types of a child whose parents are both heterozygous for "B" blood type? 8. What are the chances of a woman with Type AB and a man with Type A having a child with Type O? 9. Determine the possible genotypes & phenotypes with respect to blood type of the offspring of a couple whose blood types are A and B. Both sets of grandparents have the AB blood type.
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