Simultaneous, rapid and sensitive quantification of 8-hydroxy-2'-deoxyguanosine and cotinine in human urine

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Simultaneous, rapid and sensitive quantification of 8-hydroxy-2'-deoxyguanosine and cotinine in human urine"

Transcription

1 Analytical and Bioanalytical Chemistry Electronic Supplementary Material Simultaneous, rapid and sensitive quantification of 8-hydroxy-2'-deoxyguanosine and cotinine in human urine by on-line solid-phase extraction LC-MS/MS: correlation with tobacco exposure biomarkers NNAL Chao-Yu Chen, Yu-Ting Jhou, Hui-Ling Lee, Yu-Wen Lin 1

2 Fig. S1 Representative MRM chromatograms of LC-MS/MS analysis for total NNAL in human urine. The concentration of NNAL was ng ml -1, and the concentration of IS was 2 ng ml -1 in the human urine 2

3 Table S1 Literature basal level of 8-OHdG, cotinine and total NNAL in urine samples from smoker or non-smoker subjects Analytes Method Level (ng/mg Creatinine ± SD) Subjects Reference 8-OHdG HPLC-ECD a 4.90 ± 1.09 Non-smoker (n = 58) [1] LC-MS/MS 6.30 ± 0.20 Non-smoker (n = 96) [2] LC-MS/MS 3.72 ± 0.18 Non-smoker (n = 75) [3] ELISA b 7.36 ± 2.50 Non-smoker (n = 627) [4] On-line SPE LC-MS/MS 3.01 ± 1.67 Non-smoker (n = 40) Our study ELISA b 7.75 ± 2.80 Smoker (n = 154) [4] HPLC-ECD a 3.68 ± 0.05 Smoker (n = 104) [5] LC-MS/MS 6.90 ± 0.30 Smoker (n = 80) [2] On-line SPE LC-MS/MS 4.03 ± 1.91 Smoker (n = 40) Our study Cotinine HPLC-MS/MS 2471 (1,662-4,393) Smoker (n = 130) [6] HPLC-UV 975 ± 919 Smoker (n = 101) [7] RIA c 703 ( ) Smoker (n = 3) [8] On-line SPE LC-MS/MS ± Smoker (n = 40) Our study Total NNAL HPLC-MS/MS 0.22 ( ) Smoker (n = 130) [6] HPLC-MS/MS 0.17 ± 0.14 Smoker (n = 101) [7] HPLC-MS/MS 0.04 ( ) Smoker (n = 23) [9] GC-MS/MS 0.06 Smoker (n = 343) [10] LC-MS/MS 0.07 ± 0.04 Smoker (n = 40) Our study a High performance liquid chromatography-electrochemical detection b Enzyme-linked immunosorbent assay c Radioimmunoassay 3

4 Table S2 Comparison of free NNAL and total NNAL levels in literatures and present study Reference Group Free NNAL Ratio c Total NNAL Ratio c (ng mg -1 of creatinine) (ng mg -1 of creatinine) This study Non-smoker a (n=40) 0.03 ± ± Smoker a (n=40) 0.04 ± ± 0.04 Al Ali R et al. [9] Non-smoker a (n=28) Smoker a (n=23) Hu et al. [7] Non-smoker a (n=40) - b - b Smoker a (n=101) 0.10 ± ± 0.14 Lee et al. [11] Non-smoker a (n=69) 0.48 ± ± Smoker a (n=57) 1.06 ± ± 0.99 Chung et al. [12] Non-smoker a (n=81) 0.14 ± ± Smoker a (n=22) 0.13 ± ±0.09 a : mean ± SD, b : not mention, c: the concentration of smoker / the concentration of non-smoker. 4

5 References 1. Svoboda P, Ko SH, Cho B, Yoo SH, Choi SW, Ye SK, Kasai H, Chung MH (2008) Neopterin, a marker of immune response, and 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress, correlate at high age as determined by automated simultaneous high-performance liquid chromatography analysis of human urine. Anal Biochem 383 (2): Harman SM, Liang L, Tsitouras PD, Gucciardo F, Heward CB, Reaven PD, Ping W, Ahmed A, Cutler RG (2003) Urinary excretion of three nucleic acid oxidation adducts and isoprostane F(2)alpha measured by liquid chromatography-mass spectrometry in smokers, ex-smokers, and nonsmokers. Free Radic Biol Med 35 (10): Huang CY, Su CT, Chung CJ, Pu YS, Chu JS, Yang HY, Wu CC, Hsueh YM (2012) Urinary total arsenic and 8-hydroxydeoxyguanosine are associated with renal cell carcinoma in an area without obvious arsenic exposure. Toxicol Appl Pharmacol 262 (3): Kanaya S, Ikeya M, Yamamoto K, Moriya T, Furuhashi K, Sonoda M, Goto K, Ochi H (2004) Comparison of an oxidative stress biomarker urinary8-hydroxy-2 -deoxyguanosine, between smokers and non-smokers. BioFactors 22 (1-4): Bogdanov MB, Beal MF, McCabe DR, Griffin RM, Matson WR (1999) A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2'-deoxyguanosine measurements in urine and other biologic matrices: a one-year evaluation of methods. Free Radic Biol Med 27 (5-6): Benowitz N, Goniewicz ML, Eisner MD, Lazcano-Ponce E, Zielinska-Danch W, Koszowski B, Sobczak A, Havel C, Jacob P, 3rd (2010) Urine cotinine underestimates exposure to the tobacco-derived lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in passive compared with active smokers. Cancer Epidemiol Biomarkers Prev 19 (11): Hu CW, Hsu YW, Chen JL, Tam LM, Chao MR (2014) Direct analysis of tobacco-specific nitrosamine NNK and its metabolite NNAL in human urine by LC-MS/MS: evidence of linkage to methylated DNA lesions. Arch Toxicol 88 (2): de Weerd S, Thomas CMG, Kuster JETG, Cikot RJLM, Steegers EAP (2002) 5

6 Variation of Serum and Urine Cotinine in Passive and Active Smokers and Applicability in Preconceptional Smoking Cessation Counseling. Environ Res 90 (2): Al Ali R, Rastam S, Ibrahim I, Bazzi A, Fayad S, Shihadeh AL, Zaatari GS, Maziak W (2015) A comparative study of systemic carcinogen exposure in waterpipe smokers, cigarette smokers and non-smokers. Tob Control 24 (2): Yuan JM, Gao YT, Wang R, Chen M, Carmella SG, Hecht SS (2012) Urinary levels of volatile organic carcinogen and toxicant biomarkers in relation to lung cancer development in smokers. Carcinogenesis 33 (4): Lee HL, Hsueh YM, Chung CJ, Pu YS, Chang LW, Hsieh DPH, Liou SH, Lin P (2008) Correlation between the Urine Profile of 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone Metabolites and N7-Methylguanine in Urothelial Carcinoma Patients. Cancer Epidemiology Biomarkers & Prevention 17 (12): Chung CJ, Pu YS, Shiue HS, Lee HL, Lin P, Yang HY, Su CT, Hsueh YM (2013) 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) metabolism-related enzymes gene polymorphisms, NNK metabolites levels and urothelial carcinoma. Toxicol Lett 216 (1):

Nicotine and Toxicant Exposure among U.S. Smokeless Tobacco Users: Results from 1999 to 2012 National Health and Nutrition Examination Survey Data

Nicotine and Toxicant Exposure among U.S. Smokeless Tobacco Users: Results from 1999 to 2012 National Health and Nutrition Examination Survey Data Research Article Nicotine and Toxicant Exposure among U.S. Smokeless Tobacco Users: Results from 1999 to 2012 National Health and Nutrition Examination Survey Data Brian L. Rostron 1, Cindy M. Chang 1,

More information

Embargo Copy - Not for Distribution

Embargo Copy - Not for Distribution Page 1 of 1 Embargo Copy - Not for Distribution Adolescent Exposure to Toxic Volatile Organic Chemicals From E-Cigarettes Mark L. Rubinstein, MD, Kevin Delucchi, PhD, Neal L. Benowitz, MD, Danielle E.

More information

Assessing secondhand smoke using biological markers

Assessing secondhand smoke using biological markers TC Online First, published on September 2, 2012 as 10.1136/tobaccocontrol-2011-050298 Assessing secondhand smoke using biological markers Erika Avila-Tang, 1 Wael K Al-Delaimy, 2 David L Ashley, 3 Neal

More information

Researching Genetic Influences in Different Racial/Ethnic Populations and Cancer

Researching Genetic Influences in Different Racial/Ethnic Populations and Cancer Researching Genetic Influences in Different Racial/Ethnic Populations and Cancer Lenora WM Loo, PhD Assistant Professor (Specialist) University of Hawaii Cancer Center Off-Label Use Disclosure I do not

More information

Biomarker of Exposure Reductions Upon Switching for 5 Days from Cigarettes to a Carbon Heated Tobacco Product (CHTP 1.0)

Biomarker of Exposure Reductions Upon Switching for 5 Days from Cigarettes to a Carbon Heated Tobacco Product (CHTP 1.0) Biomarker of Exposure Reductions Upon Switching for Days from to a Carbon Heated Tobacco Product () A. Donelli, C. Tran, C. Haziza, J. Ancerewicz, G. de La Bourdonnaye, R. Weitkunat, and F. Lüdicke 8 healthy

More information

Randomized Trial of Reduced-Nicotine Standards for Cigarettes

Randomized Trial of Reduced-Nicotine Standards for Cigarettes The new england journal of medicine Special Article Randomized Trial of Reduced-Nicotine Standards for Cigarettes Eric C. Donny, Ph.D., Rachel L. Denlinger, B.S., Jennifer W. Tidey, Ph.D., Joseph S. Koopmeiners,

More information

REVIEW. Tobacco Smoke Carcinogens and Lung Cancer. Stephen S. Hecht

REVIEW. Tobacco Smoke Carcinogens and Lung Cancer. Stephen S. Hecht REVIEW Tobacco Smoke Carcinogens and Lung Cancer Stephen S. Hecht The complexity of tobacco smoke leads to some confusion about the mechanisms by which it causes lung cancer. Among the multiple components

More information

formaldehyde S-hydroxymethyl-glutatione S-formylglutatione Formic acid CO 2 + H 2 O (exhaled) Na + HCOO - (urine)

formaldehyde S-hydroxymethyl-glutatione S-formylglutatione Formic acid CO 2 + H 2 O (exhaled) Na + HCOO - (urine) Roberto Bono roberto.bono@unito.it Biological monitoring of exposure to formaldehyde. Different mechanisms of action; formation of protein adduct and evidence of oxidative stress formaldehyde Although

More information

DRUG TESTING POLICY. Policy Number: ADMINISTRATIVE T0 Effective Date: January 1, Related Policies None

DRUG TESTING POLICY. Policy Number: ADMINISTRATIVE T0 Effective Date: January 1, Related Policies None DRUG TESTING POLICY UnitedHealthcare Oxford Reimbursement Policy Policy Number: ADMINISTRATIVE 259.2 T0 Effective Date: January 1, 2018 Table of Contents Page INSTRUCTIONS FOR USE... 1 APPLICABLE LINES

More information

SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine

SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine SPE-LC-MS/MS Method for the Determination of Nicotine, Cotinine, and Trans-3-hydroxycotinine in Urine J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 709 Key Words SPE, SOLA

More information

Isoprostanes: Biomarkers for Oxidative Stress

Isoprostanes: Biomarkers for Oxidative Stress Isoprostanes: Biomarkers for Oxidative Stress Denis Callewaert, Ph.D. President, Oxford Biomedical Research, Inc. Professor Emeritus, Oakland University 2012 Oxford Biomedical Research, Inc. All rights

More information

Smoking and Mortality in the Japan Collaborative Cohort Study for Evaluation of Cancer (JACC)

Smoking and Mortality in the Japan Collaborative Cohort Study for Evaluation of Cancer (JACC) Smoking and Mortality SECTION 6 Smoking and Mortality in the Japan Collaborative Cohort Study for Evaluation of Cancer (JACC) Kotaro Ozasa Abstract In the JACC study, risk of death with all cancers and

More information

Exposure to Nicotine and Carcinogens among Southwestern Alaskan Native Cigarette Smokers and Smokeless Tobacco Users

Exposure to Nicotine and Carcinogens among Southwestern Alaskan Native Cigarette Smokers and Smokeless Tobacco Users Research Article Cancer Epidemiology, Biomarkers & Prevention Exposure to Nicotine and Carcinogens among Southwestern Alaskan Native Cigarette Smokers and Smokeless Tobacco Users Neal L. Benowitz 1, Caroline

More information

The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides

The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides The Comparison of High Resolution MS with Triple Quadrupole MS for the Analysis of Oligonucleotides Mohammed Abrar Unilabs York Bioanalytical Solutions Outline Introduction Why LC-MS/MS? Limitations of

More information

DRUG TESTING POLICY. Policy Number: ADMINISTRATIVE T0 Effective Date: October 1, Related Policies None

DRUG TESTING POLICY. Policy Number: ADMINISTRATIVE T0 Effective Date: October 1, Related Policies None DRUG TESTING POLICY UnitedHealthcare Oxford Reimbursement Policy Policy Number: ADMINISTRATIVE 259.1 T0 Effective Date: October 1, 2017 Table of Contents Page INSTRUCTIONS FOR USE... 1 APPLICABLE LINES

More information

PhD student Gregorio Peron e-mail: gregorio.peron@studenti.unipd.it tel.: 347 4802865 Proposed supervisor Dott. Stefano Dall Acqua e-mail: stefano.dallacqua@unipd.it tel.: 049 8275332 Project title English:

More information

Self-Reported Exposure to Second-Hand Smoke and Positive Urinary Cotinine in Pregnant Nonsmokers

Self-Reported Exposure to Second-Hand Smoke and Positive Urinary Cotinine in Pregnant Nonsmokers Original Article DOI 10.3349/ymj.2009.50.3.345 pissn: 0513-5796, eissn: 1976-2437 Yonsei Med J 50(3): 345-351, 2009 Self-Reported Exposure to Second-Hand Smoke and Positive Urinary Cotinine in Pregnant

More information

Significant Positive Correlation of Plasma BPDE-Albumin Adducts to Urinary 1-Hydroxypyrene in Coke Oven Workers 1

Significant Positive Correlation of Plasma BPDE-Albumin Adducts to Urinary 1-Hydroxypyrene in Coke Oven Workers 1 BIOMEDICAL AND ENVIRONMENTAL SCIENCES 20, 179-183 (2007) Significant Positive Correlation of Plasma BPDE-Albumin Adducts to Urinary 1-Hydroxypyrene in Coke Oven Workers 1 HONG WANG *,+, XIAO-BO YANG *,

More information

Chronic infections with hepatitis B and hepatitis C

Chronic infections with hepatitis B and hepatitis C Original Article / Liver The impact of family history of hepatocellular carcinoma on its patients' survival Wing Chiu Dai, Sheung Tat Fan, Tan To Cheung, Kenneth SH Chok, Albert CY Chan, Simon HY Tsang,

More information

Cigarettes Determination of tobacco specific nitrosamines in mainstream cigarette smoke Method using LC-MS/MS

Cigarettes Determination of tobacco specific nitrosamines in mainstream cigarette smoke Method using LC-MS/MS INTERNATIONAL STANDARD ISO 19290 First edition 2016-12-01 Cigarettes Determination of tobacco specific nitrosamines in mainstream cigarette smoke Method using LC-MS/MS Cigarettes Dosage des nitrosamines

More information

Investigating the recheck rules for urine analysis in children

Investigating the recheck rules for urine analysis in children Investigating the recheck rules for urine analysis in children Y.M. He*, S.W. Yao*, Y.J. Huang, B.S. Liang and H.Y. Liu Clinical Laboratory, Guangzhou Women and Children s Medical Center, Guangzhou Medical

More information

Proceedings: Indoor Air 2002

Proceedings: Indoor Air 2002 ASSESSMENT OF ENVIRONMENTAL TOBACCO SMOKE (ETS) EXPOSURE: URINARY COTININE CONCENTRATIONS IN CHILDREN ARE STRONGLY ASSOCIATED WITH THE HOUSE DUST CONCENTRATIONS OF NICOTINE AT HOME S Willers 1*, HO Hein

More information

ANALYTICAL METHODS IN DETERMINING AFLATOXIN B1 IN FOOD

ANALYTICAL METHODS IN DETERMINING AFLATOXIN B1 IN FOOD 1 International Journal of Advance Research, IJOAR.org Volume 1, Issue 4, April 2013, ISSN 2320-9186 ANALYTICAL METHODS IN DETERMINING AFLATOXIN B1 IN FOOD MATERIALS Melvin Samuel. S * * School of Biosciences

More information

Fast and simultaneous analysis of ethanol metabolites and barbiturates using the QTRAP 4500 LC-MS/MS system

Fast and simultaneous analysis of ethanol metabolites and barbiturates using the QTRAP 4500 LC-MS/MS system Fast and simultaneous analysis of ethanol metabolites and barbiturates using the QTRAP 4500 LC-MS/MS system Xiang He 1, Adrian Taylor 2 and Alexandre Wang 1 1 SCIEX, Redwood City, USA. 2 SCIEX, Concord,

More information

Objective research on tongue manifestation of patients with eczema

Objective research on tongue manifestation of patients with eczema Technology and Health Care 25 (2017) S143 S149 DOI 10.3233/THC-171316 IOS Press S143 Objective research on tongue manifestation of patients with eczema Zhifeng Yu, Haifang Zhang, Linjie Fu and Xiaozuo

More information

SCOPE OF ACCREDITATION TO ISO/IEC 17025:2005. ENTHALPY ANALYTICAL, INC East Parham Road Richmond, VA Gwen Vaughan Phone:

SCOPE OF ACCREDITATION TO ISO/IEC 17025:2005. ENTHALPY ANALYTICAL, INC East Parham Road Richmond, VA Gwen Vaughan Phone: SCOPE OF ACCREDITATION TO ISO/IEC 17025:2005 ENTHALPY ANALYTICAL, INC. 1470 East Parham Road Richmond, VA 23228 Gwen Vaughan Phone: 804 887 2104 CHEMICAL Valid To: November 30, 2019 Certificate Number:

More information

Current Developments in Guidance for Regulatory Bioanalysis

Current Developments in Guidance for Regulatory Bioanalysis Current Developments in Guidance for Regulatory Bioanalysis Dr Gerry McGuire Resolution Analytical Consultancy Ltd Edinburgh, Scotland UK gerry@resolutioneurope.com 1st MENA Regulatory Conference on Bioequivalence,

More information

Drug Testing Policy. Reimbursement Policy CMS Approved By. Policy Number. Annual Approval Date. Reimbursement Policy Oversight Committee

Drug Testing Policy. Reimbursement Policy CMS Approved By. Policy Number. Annual Approval Date. Reimbursement Policy Oversight Committee Reimbursement Policy CMS 1500 Drug Testing Policy Policy Number 2017R6005A Annual Approval Date 05/10/2017 Approved By Reimbursement Policy Oversight Committee IMPORTANT NOTE ABOUT THIS REIMBURSEMENT POLICY

More information

Harmful and potentially harmful constituents in e-cigarettes

Harmful and potentially harmful constituents in e-cigarettes Harmful and potentially harmful constituents in e-cigarettes Prof. Riccardo Polosa Institute of Internal Medicine Centre for the Prevention and Cure of Tabagism University of Catania - ITALY Global Forum

More information

67:78-83, 2005 (SCI).

67:78-83, 2005 (SCI). * 董石城醫師 所有發表期刊論文及演講 1. Lu YC, Wang PW, Liu RT, Tung SC, Chien WY, Hung SL and Chen M: Limited joint mobility of the hand: prevalence and relation to chronic complications in non-insulin-dependent diabetes

More information

Alimentación, nutrición y dietética GLUCOSINOLATES AND HUMAN HEALTH.

Alimentación, nutrición y dietética GLUCOSINOLATES AND HUMAN HEALTH. GLUCOSINOLATES AND HUMAN HEALTH. Ian.T. Johnson Institute of Food Research. Norwich Research Park Colney, NORWICH. NR4 7UA, UK Tel. +44 (0)1603 255330 ian.johnson@bbsrc.ac.uk Abstract The glucosinolates

More information

Pharmacokinetics of daptomycin in a patient with severe renal failure not under dialysis

Pharmacokinetics of daptomycin in a patient with severe renal failure not under dialysis AAC Accepts, published online ahead of print on 1 April 2013 Antimicrob. Agents Chemother. doi:10.1128/aac.00230-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 Pharmacokinetics

More information

VARIABLE CYP2A6-MEDIATED NICOTINE METABOLISM ALTERS SMOKING BEHAVIOR AND RISK

VARIABLE CYP2A6-MEDIATED NICOTINE METABOLISM ALTERS SMOKING BEHAVIOR AND RISK 0090-9556/01/2904-548 552$3.00 DRUG METABOLISM AND DISPOSITION Vol. 29, No. 4, Part 2 Copyright 2001 by The American Society for Pharmacology and Experimental Therapeutics 290118/894071 DMD 29:548 552,

More information

Genome-wide association study of esophageal squamous cell carcinoma in Chinese subjects identifies susceptibility loci at PLCE1 and C20orf54

Genome-wide association study of esophageal squamous cell carcinoma in Chinese subjects identifies susceptibility loci at PLCE1 and C20orf54 CORRECTION NOTICE Nat. Genet. 42, 759 763 (2010); published online 22 August 2010; corrected online 27 August 2014 Genome-wide association study of esophageal squamous cell carcinoma in Chinese subjects

More information

EMBARGOED FOR RELEASE UNTIL 5:00 PM ET ON MONDAY, FEBRUARY 6, 2017

EMBARGOED FOR RELEASE UNTIL 5:00 PM ET ON MONDAY, FEBRUARY 6, 2017 Annals of Internal Medicine ORIGINAL RESEARCH Nicotine, Carcinogen, and Toxin Exposure in Long-Term E-Cigarette and Nicotine Replacement Therapy Users A Cross-sectional Study Lion Shahab, PhD; Maciej L.

More information

Tea and Cancer Introduction What is cancer? Causes of cancer Diet and cancer

Tea and Cancer Introduction What is cancer? Causes of cancer Diet and cancer Tea and Cancer Introduction Cancer is a major cause of morbidity in the UK with over 200,000 newly diagnosed cases, and around 120,000 deaths from cancer each year. It is expected that more than one in

More information

Main Challenges Related to Measuring Biomarkers of Exposure of Bisphenol A and Triclosan

Main Challenges Related to Measuring Biomarkers of Exposure of Bisphenol A and Triclosan Main Challenges Related to Measuring Biomarkers of Exposure of Bisphenol A and Triclosan Antonia M. Calafat, PhD Organic Analytical Toxicology Branch Division of Laboratory Sciences National Center for

More information

Incomplete Hepatitis B Immunization, Maternal Carrier Status, and Increased Risk of Liver Diseases: A 20-Year Cohort Study of 3.8 Million Vaccinees

Incomplete Hepatitis B Immunization, Maternal Carrier Status, and Increased Risk of Liver Diseases: A 20-Year Cohort Study of 3.8 Million Vaccinees Incomplete Hepatitis B Immunization, Maternal Carrier Status, and Increased Risk of Liver Diseases: A 20-Year Cohort Study of 3.8 Million Vaccinees Yin-Chu Chien, 1 Chyi-Feng Jan, 2 Chun-Ju Chiang, 3 Hsu-Sung

More information

Members of The Chinese Medicine Council of Hong Kong, Chinese Medicine Practitioners Board, Chinese Medicines Board and its Committees

Members of The Chinese Medicine Council of Hong Kong, Chinese Medicine Practitioners Board, Chinese Medicines Board and its Committees Members of The Chinese Medicine Council of Hong Kong, Chinese Medicine Practitioners Board, Chinese Medicines Board and its Committees and of the Chinese Medicine Council of Hong Kong (e) (f) (g) a chairman;

More information

Electronic Cigarettes, Nicotine and Policy Implications

Electronic Cigarettes, Nicotine and Policy Implications Electronic Cigarettes, Nicotine and Policy Implications Neal L Benowitz MD University of California San Francisco The E-Cigarette Summit The Royal Society London November 17, 2016 Disclosures Dr Benowitz

More information

Urinary Nucleosides as Biomarkers of Breast, Colon, Lung, and Gastric Cancer in Taiwanese

Urinary Nucleosides as Biomarkers of Breast, Colon, Lung, and Gastric Cancer in Taiwanese Urinary Nucleosides as Biomarkers of Breast, Colon, Lung, and Gastric Cancer in Taiwanese Wei-Yi Hsu 1,2, Chao-Jung Chen 1,3, Yu-Chuen Huang 1,4, Fuu-Jen Tsai 1,5, Long-Bin Jeng 6 *, Chien- Chen Lai 7,8

More information

Modulation of aflatoxin biomarkers in human blood and urine by green tea polyphenols intervention

Modulation of aflatoxin biomarkers in human blood and urine by green tea polyphenols intervention Carcinogenesis vol.29 no.2 pp.411 417, 2008 doi:10.1093/carcin/bgn008 Advance Access publication January 12, 2008 Modulation of aflatoxin biomarkers in human blood and urine by green tea polyphenols intervention

More information

Genetics of Nicotine Dependence & Impact of Smoking on Bladder Cancer Risk and Prognosis

Genetics of Nicotine Dependence & Impact of Smoking on Bladder Cancer Risk and Prognosis Genetics of Nicotine Dependence & Impact of Smoking on Bladder Cancer Risk and Prognosis BCAN August 2014 Helena Furberg Barnes Assistant Attending Epidemiologist www.mskcc.org Current smoking in the US

More information

9. Richie, J.P., Jr. (1989) Antioxidants and the free radical theory of aging. Assoc. Anthropol. Gerontol. Newsletter 10:4-5.

9. Richie, J.P., Jr. (1989) Antioxidants and the free radical theory of aging. Assoc. Anthropol. Gerontol. Newsletter 10:4-5. John Richie, Ph.D. All Publications 1. Richie, J.P., Jr., Mills, B.J., and Lang, C.A. (1984) The verification of a mammalian toxicant classification using a mosquito screening. Fundam. Appl. Toxicol. 4:1029-1035.

More information

for Tobacco Use: Comparison of Users of Cigarettes, Smokeless Tobacco, Cigars, and Pipes

for Tobacco Use: Comparison of Users of Cigarettes, Smokeless Tobacco, Cigars, and Pipes Minor Tobacco Alkaloids as Biomarkers for Tobacco Use: Comparison of Users of Cigarettes, Smokeless Tobacco, Cigars, and Pipes Peyton Jacob III, PhD, Lisa Yu, BS, Alexander T Shulgin, PhD, and Neal L.

More information

Institute of Toxicology Clinical Toxicology and Pharmacology Berliner Betrieb für Zentrale Gesundheitliche Aufgaben, Berlin

Institute of Toxicology Clinical Toxicology and Pharmacology Berliner Betrieb für Zentrale Gesundheitliche Aufgaben, Berlin LOGO Institute of Toxicology Clinical Toxicology and Pharmacology Berliner Betrieb für Zentrale Gesundheitliche Aufgaben, Berlin Rapid Quantification of Tilidine, Nortilidine and Bisnortilidine in Urine

More information

Development and Validation of UV- Spectrophotometric Method for Estimation of Simvastatin in Bulk and Solid Dosage Form.

Development and Validation of UV- Spectrophotometric Method for Estimation of Simvastatin in Bulk and Solid Dosage Form. Development and Validation of UV- Spectrophotometric Method for Estimation of Simvastatin in Bulk and Solid Dosage Form. Amit E. Birari * Departments of Pharmaceutics, Loknete Dr.J.D.Pawar College of Pharmacy

More information

Nicotine and Cotinine Levels With Electronic Cigarette: A Review

Nicotine and Cotinine Levels With Electronic Cigarette: A Review Review Nicotine and Cotinine Levels With Electronic Cigarette: A Review International Journal of Toxicology 2016, Vol. 35(2) 179-185 ª The Author(s) 2015 Reprints and permission: sagepub.com/journalspermissions.nav

More information

Gene environment interaction in tobacco-related cancers

Gene environment interaction in tobacco-related cancers Carcinogenesis vol.29 no.8 pp.1467 1474, 2008 doi:10.1093/carcin/bgn062 Advance Access publication June 12, 2008 REVIEW Gene environment interaction in tobacco-related cancers Emanuela Taioli Department

More information

Jieying Jiang, Ph.D. Yu Chen, Ph.D., MPH Habibul Ahsan, M.D., MMedSc

Jieying Jiang, Ph.D. Yu Chen, Ph.D., MPH Habibul Ahsan, M.D., MMedSc Jieying Jiang, Ph.D. Yu Chen, Ph.D., MPH Habibul Ahsan, M.D., MMedSc Emerging Epidemic of CVD in Developing Countries Developed countries Relative contribution of CVD deaths to total mortality in 1990

More information

Electronic cigarette use and harm reversal: emerging evidence in health and disease

Electronic cigarette use and harm reversal: emerging evidence in health and disease Electronic cigarette use and harm reversal: emerging evidence in health and disease Prof. Riccardo Polosa Institute of Internal Medicine University of Catania ITALY Exhaled CO in ECIG users R. Polosa et

More information

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids Jeremy Netto, 1 Stephen Wong, 1 Federico Torta, 2 Pradeep Narayanaswamy, 2

More information

New Automated Screening System for the Determination of Basic Compounds in Urine by On-Line Extraction-HPLC- DAD

New Automated Screening System for the Determination of Basic Compounds in Urine by On-Line Extraction-HPLC- DAD New Automated Screening System for the Determination of Basic Compounds in Urine by On-Line Extraction-HPLC- DAD Thomas Grobosch, Lena Schönberg, Dagmar Lampe, Charlotte Kloft Abstract A new automated,

More information

RESUME Sep National Medical License, Taiwan Oct Board of Interal Medicine, Taiwan Aug Board of Cardiology, Internal Medicine, Taiwan

RESUME Sep National Medical License, Taiwan Oct Board of Interal Medicine, Taiwan Aug Board of Cardiology, Internal Medicine, Taiwan RESUME Ye-Hsu Lu, M.D. Office Address: No.100, Ziyou 1st Rd., Sanmin Dist., Kaohsiung City 80708, Taiwan TEL: +886-7-3121101 ext. 7741 (Office), +886-918867017 (Cell) FAX: +886-7-3234845 E-mail: yehslu@gmail.com

More information

5. Summary of Data Reported and Evaluation

5. Summary of Data Reported and Evaluation SMOKELESS TOBACCO 363 5. Summary of Data Reported and Evaluation 5.1 Exposure data The use of tobacco dates back to at least 5000 years in the Americas, and, by the 1700s, had spread throughout the world.

More information

Rapid and Robust Detection of THC and Its Metabolites in Blood

Rapid and Robust Detection of THC and Its Metabolites in Blood Rapid and Robust Detection of THC and Its Metabolites in Blood Application Note Forensics/Doping Control Author Stephan Baumann Agilent Technologies, Inc. Santa Clara CA 95051 USA Abstract A robust method

More information

Specific Detection of Anabasine, Nicotine, and Nicotine Metabolites in Urine by Liquid Chromatography Tandem Mass Spectrometry

Specific Detection of Anabasine, Nicotine, and Nicotine Metabolites in Urine by Liquid Chromatography Tandem Mass Spectrometry Clinical Chemistry / DETECTION OF TOBACCO ALKALOIDS IN URINE Specific Detection of Anabasine, Nicotine, and Nicotine Metabolites in Urine by Liquid Chromatography Tandem Mass Spectrometry Andrew N. Hoofnagle,

More information

Detection of Low Level of Chloramphenicol in Milk and Honey with MIP SPE and LC-MS-MS

Detection of Low Level of Chloramphenicol in Milk and Honey with MIP SPE and LC-MS-MS Detection of Low Level of Chloramphenicol in Milk and Honey with MIP SPE and LC-MS-MS Olga Shimelis, An Trinh, and Michael Ye Supelco, Div. of Sigma-Aldrich, Bellefonte, PA T407125 Introduction Molecularly

More information

Overview. Introduction. Experimental. Cliquid Software for Routine LC/MS/MS Analysis

Overview. Introduction. Experimental. Cliquid Software for Routine LC/MS/MS Analysis A Fast and Sensitive LC/MS/MS Method for the Quantification and Confirmation of 3 Benzodiazepines and Nonbenzodiazepine Hypnotics in Forensic Urine Samples Cliquid Software for Routine LC/MS/MS Analysis

More information

Advances in Environmental Biology

Advances in Environmental Biology Advances in Environmental Biology, 9() November 15, Pages: 191-195 AENSI Journals Advances in Environmental Biology ISSN-1995-75 EISSN-199-1 Journal home page: http://www.aensiweb.com/aeb/ Effects of Active

More information

PHARMACOKINETIC STUDY OF DEXTROMETHORPHAN WITH URINARY EXCRETION

PHARMACOKINETIC STUDY OF DEXTROMETHORPHAN WITH URINARY EXCRETION PHARMACOKINETIC STUDY OF DEXTROMETHORPHAN WITH URINARY EXCRETION Heesun CHUNG, Wonkyung YANG, Hwakyung CHOI, Wontack JIN, Sihnyoung SIHN, Youngchan YOO National Institute of Scientific Investigation, Seoul,

More information

Environmental and Biological Assessment of Environmental Tobacco Smoke Exposure Among Casino Dealers

Environmental and Biological Assessment of Environmental Tobacco Smoke Exposure Among Casino Dealers Workplace Safety and Health Environmental and Biological Assessment of Environmental Tobacco Smoke Exposure Among Casino Dealers Chandran Achutan, PhD Christine West, RN, MSN, MPH Charles Mueller, MS Yvonne

More information

Determination of red blood cell fatty acid profiles in clinical research

Determination of red blood cell fatty acid profiles in clinical research Application Note Clinical Research Determination of red blood cell fatty acid profiles in clinical research Chemical ionization gas chromatography tandem mass spectrometry Authors Yvonne Schober 1, Hans

More information

How to improve analytical strategies to monitor growth promoting agents misuse in cattle

How to improve analytical strategies to monitor growth promoting agents misuse in cattle 5th interantional Symposium on Recent Advances in Food Analysis Prague, Czech Republic, 1-4 November 2011 How to improve analytical strategies to monitor growth promoting agents misuse in cattle Emmanuelle

More information

Application Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products

Application Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products High-Performance Anion-Exchange Chromatography coupled with Pulsed Electrochemical Detection as a powerful tool to evaluate lactose content in lactose-free labeled products Application Note Authors: C.

More information

Pancreatic adenocarcinoma is the fourth leading cause of. Pancreatic Cancer Patients Who Smoke and Drink Are Diagnosed at Younger Ages

Pancreatic adenocarcinoma is the fourth leading cause of. Pancreatic Cancer Patients Who Smoke and Drink Are Diagnosed at Younger Ages CLINICAL GASTROENTEROLOGY AND HEPATOLOGY 2009;7:1007 1012 Pancreatic Cancer Patients Who Smoke and Drink Are Diagnosed at Younger Ages RANDALL E. BRAND,* JULIA B. GREER,* EUGENE ZOLOTAREVSKY, RHONDA BRAND,*

More information

Carcinogenicity studies of inhaled cigarette smoke in laboratory animals: old and new

Carcinogenicity studies of inhaled cigarette smoke in laboratory animals: old and new Carcinogenesis vol.26 no.9 pp.1488 1492, 2005 doi:10.1093/carcin/bgi148 Advance Access publication June 1, 2005 COMMENTARY Carcinogenicity studies of inhaled cigarette smoke in laboratory animals: old

More information

Supplemental Information

Supplemental Information Supplemental Information Tobacco-specific Carcinogen Induces DNA Methyltransferases 1 Accumulation through AKT/GSK3β/βTrCP/hnRNP-U in Mice and Lung Cancer patients Ruo-Kai Lin, 1 Yi-Shuan Hsieh, 2 Pinpin

More information

Cardiovascular effects of nicotine vs. cigarette smoke

Cardiovascular effects of nicotine vs. cigarette smoke Cardiovascular effects of nicotine vs. cigarette smoke Dr Konstantinos Farsalinos Researcher Onassis Cardiac Surgery Center, Greece University of Patras, Greece National School of Public Health, Athens-Greece

More information

E-CIGARETTES CANCER INSIGHT WHAT YOU NEED TO KNOW ABOUT. FOR GPs. INSIDE: A3 poster to display in your practice. October Created by Freepik

E-CIGARETTES CANCER INSIGHT WHAT YOU NEED TO KNOW ABOUT. FOR GPs. INSIDE: A3 poster to display in your practice. October Created by Freepik Created by Freepik CANCER INSIGHT FOR GPs October 07 WHAT YOU NEED TO KNOW ABOUT GARETTES INSIDE: A poster to display in your practice ARE FAR SAFER THAN SMOKING E-cigarettes contain nicotine but not cancer

More information

Assessing smoking status in children, adolescents and adults: cotinine cutpoints revisited.

Assessing smoking status in children, adolescents and adults: cotinine cutpoints revisited. Assessing smoking status in children, adolescents and adults: cotinine cutpoints revisited. Martin J Jarvis, Jennifer Fidler Health Behaviour Research Centre Department of Epidemiology and Public Health

More information

Drug Testing Policy. Approved By 05/10/2017. Application This reimbursement policy applies to UnitedHealthcare Community Plan Medicaid products.

Drug Testing Policy. Approved By 05/10/2017. Application This reimbursement policy applies to UnitedHealthcare Community Plan Medicaid products. Policy Number 2017R6005B Annual Approval Date Drug Testing Policy 05/10/2017 Approved By Reimbursement Policy Oversight Committee IMPORTANT NOTE ABOUT THIS REIMBURSEMENT POLICY You are responsible for

More information

[application note] Simultaneous detection and quantification of D 9 THC, 11-OH-D 9 T H C and D 9 THC-COOH in whole blood by GC tandem quadrupole MS

[application note] Simultaneous detection and quantification of D 9 THC, 11-OH-D 9 T H C and D 9 THC-COOH in whole blood by GC tandem quadrupole MS Simultaneous detection and quantification of D 9 THC, 11-OH-D 9 T H C and D 9 THC-COOH in whole blood by GC tandem quadrupole MS Marie Bresson, Vincent Cirimele, Pascal Kintz, Marion Villain; Laboratoire

More information

Introduction. Abstract

Introduction. Abstract Validation of a Liquid Chromatography Tandem Mass Spectrometry Method for the Detection of Nicotine Biomarkers in Hair and an Evaluation of Wash Procedures for Removal of Environmental Nicotine Eleanor

More information

A Fast Efficient Method to Determine the Presence of Nitrosamines in Cosmetics, Personal Care, and Consumer Products

A Fast Efficient Method to Determine the Presence of Nitrosamines in Cosmetics, Personal Care, and Consumer Products A Fast Efficient Method to Determine the Presence of itrosamines in Cosmetics, Personal Care, and Consumer Products Michael Jones 1 and Christopher Glover 2 1 Waters Corporation, Manchester, UK 2 orthumbria

More information

Exploitation of Epigenetic Changes to Distinguish Benign from Malignant Prostate Biopsies

Exploitation of Epigenetic Changes to Distinguish Benign from Malignant Prostate Biopsies Exploitation of Epigenetic Changes to Distinguish Benign from Malignant Prostate Biopsies Disclosures MDxHealth Scientific Advisor 2 Case Study 54-year-old man referred for a PSA of 7 - Healthy, minimal

More information

Secondhand smoke (SHS) causes a

Secondhand smoke (SHS) causes a JOEM Volume 48, Number 2, February 26 181 Correlating Atmospheric and Biological Markers in Studies of Secondhand Tobacco Smoke Exposure and Dose in Children and Adults James Repace, MSc Wael K. Al-Delaimy,

More information

Predictors of Cigarette Smoking Behavior Among Military University Students in Taiwan. Wang, Kwua-Yun; Yang, Chia-Chen

Predictors of Cigarette Smoking Behavior Among Military University Students in Taiwan. Wang, Kwua-Yun; Yang, Chia-Chen The Henderson Repository is a free resource of the Honor Society of Nursing, Sigma Theta Tau International. It is dedicated to the dissemination of nursing research, researchrelated, and evidence-based

More information

Perception of Electronic Cigarette Use and Effects on Division III Athletes

Perception of Electronic Cigarette Use and Effects on Division III Athletes Perception of Electronic Cigarette Use and Effects on Division III Athletes Byron Campbell Damian Pulos Kimberly Pritchard PhD, LAT, ATC Michael Kotelnicki MS, ATC Introduction An electronic cigarette

More information

Changing Prevalence of Anti-Hepatitis A Virus in Adolescents in A Rural Township in Taiwan

Changing Prevalence of Anti-Hepatitis A Virus in Adolescents in A Rural Township in Taiwan Original Article 321 Changing Prevalence of Anti-Hepatitis A Virus in Adolescents in A Rural Township in Taiwan Jing-Yi Chen 1,2,5, MD; Jui-Chin Chiang 1,2,6, MD; Sheng-Nan Lu 2,3,4, MD, MPH, PhD; Shu-Fen

More information

Arsenic in Food. Presented by Michelle Briscoe President & Technical Director Brooks Rand Labs

Arsenic in Food. Presented by Michelle Briscoe President & Technical Director Brooks Rand Labs Arsenic in Food www.brooksrand.com Presented by Michelle Briscoe President & Technical Director Brooks Rand Labs Presented November 8, 2013 Costco International QA & Food Safety Summit Issaquah, Washington

More information

The Family Smoking Prevention and Tobacco

The Family Smoking Prevention and Tobacco Call to Establish Constituent Standards for Smokeless Tobacco Products Irina Stepanov, PhD Dorothy Hatsukami, PhD Objectives: To summarize evidence supporting the establishment of constituent standards

More information

Experience of Korea Acute Myocardial Infarction Registry (KAMIR)

Experience of Korea Acute Myocardial Infarction Registry (KAMIR) Japan-Korea Joint AMI Symposium Korean Society of Myocardial Infarction Apr 18-19 2014 Experience of Korea Acute Myocardial Infarction Registry (KAMIR) Myung Ho Jeong, MD, PhD, FACC, FAHA, FESC, FSCAI,

More information

Case-Control Studies

Case-Control Studies Case-Control Studies Marc Schenker M.D., M.P.H Dept. of Public Health Sciences UC Davis Marc Schenker M.D., M.P.H, UC Davis 1 Case-Control Studies OBJECTIVES After this session, you will be familiar with:

More information

Misclassification Rates for Current

Misclassification Rates for Current ... :... ::.}-.,.....,... - :.,.....-.:... ; -. ;..-. : -... -i-....... fd g C? 00 --0 f -S g. : 0 f.x-l,z American OrI99S,Vot 88>*.:10- - Misclassification Rates for Current Smokers Misclassified as Nonsmokers

More information

Carbaryl in Drinking Water. Background document for development of WHO Guidelines for Drinking-Water Quality

Carbaryl in Drinking Water. Background document for development of WHO Guidelines for Drinking-Water Quality Carbaryl in Drinking Water Background document for development of WHO Guidelines for Drinking-Water Quality This document is based on Chemical Health ands Safety Guide number 78 (1992) and JMPR pesticide

More information

Original Research Evaluation of DNA Damage in People Occupationally Exposed to Arsenic and Some Heavy Metals

Original Research Evaluation of DNA Damage in People Occupationally Exposed to Arsenic and Some Heavy Metals Polish J. of Environ. Stud. Vol. 18, No. 6 (2009), 1131-1139 Original Research Evaluation of DNA Damage in People Occupationally Exposed to Arsenic and Some Heavy Metals A. Szymańska-Chabowska*, A. Beck,

More information

Enzymes involved in the bioactivation of 4-(methylnitrosamino)-1- (3-pyridyl)-1-butanone in patas monkey lung and liver microsomes

Enzymes involved in the bioactivation of 4-(methylnitrosamino)-1- (3-pyridyl)-1-butanone in patas monkey lung and liver microsomes Carcinogenesis vol.18 no.8 pp.1577 1584, 1997 Enzymes involved in the bioactivation of 4-(methylnitrosamino)-1- (3-pyridyl)-1-butanone in patas monkey lung and liver microsomes Theresa J.Smith 1,3, Anita

More information

Regular Light CHA-E M -0 7 0 0 2 2 5 0 Acetone Butane Arsenic( ) Cadmium Toluene,, 1. National Toxicology Program. 11th Report on Carcinogens; 2005. Available at: http://ntp-server.niehs.nih.gov. 2. Mackay

More information

Inferring causality in observational epidemiology: Breast Cancer Risk as an Example

Inferring causality in observational epidemiology: Breast Cancer Risk as an Example Inferring causality in observational epidemiology: Breast Cancer Risk as an Example Mary Beth Terry, PhD Department of Epidemiology and Environmental Sciences Cancer Genes vs Environmental Risk Factors

More information

Lipids Analysis. Lipids

Lipids Analysis. Lipids Lipids Analysis Stephen Barnes 3 5 15 Lipids Lipids are mostly very hydrophobic Most are conjugates of fatty acids of a variety of chain lengths, which have different degrees of unsaturation, cis trans

More information

Applications for ESA Products

Applications for ESA Products Page 1 Applications for ESA Products How do Iknow if my compound is electroactive? Click here: to view amodel electroactive compound or to select aclass of compounds to review known electroactive species.

More information

Core E Analysis of Neutral Lipids from Human Plasma June 4, 2010 Thomas J. Leiker and Robert M. Barkley

Core E Analysis of Neutral Lipids from Human Plasma June 4, 2010 Thomas J. Leiker and Robert M. Barkley Core E Analysis of Neutral Lipids from Human Plasma June 4, 2010 Thomas J. Leiker and Robert M. Barkley This protocol describes the extraction and direct measurement of cholesterol esters (CEs) and triacylglycerols

More information

Kidney Cancer Causes, Risk Factors, and Prevention

Kidney Cancer Causes, Risk Factors, and Prevention Kidney Cancer Causes, Risk Factors, and Prevention Risk Factors A risk factor is anything that affects your chance of getting a disease such as cancer. Learn more about the risk factors for kidney cancer.

More information

Ion fragmentation of small molecules in mass spectrometry

Ion fragmentation of small molecules in mass spectrometry Ion fragmentation of small molecules in mass spectrometry Jeevan Prasain jprasain@uab.edu 6-2612 Nomenclature: the main names and acronyms used in mass spectrometry Molecular ion: Ion formed by addition

More information

CYP1A2-163C/A (rs762551) polymorphism and bladder cancer risk: a case-control study

CYP1A2-163C/A (rs762551) polymorphism and bladder cancer risk: a case-control study CYP1A2-163C/A (rs762551) polymorphism and bladder cancer risk: a case-control study Y.L. Song 1,2, L. Wang 2, J.C. Ren 1 and Z.H. Xu 1 1 Department of Urology, Qilu Hospital, Shandong University, Jinan,

More information

Urinary biomarkers of smokers exposure to tobacco smoke constituents in tobacco products assessment: a fit for purpose approach

Urinary biomarkers of smokers exposure to tobacco smoke constituents in tobacco products assessment: a fit for purpose approach Biomarkers ISSN: 1354-750X (Print) 1366-5804 (Online) Journal homepage: http://www.tandfonline.com/loi/ibmk20 Urinary biomarkers of smokers exposure to tobacco smoke constituents in tobacco products assessment:

More information