Cigarette Smoke Generator

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1 Sophisticated Life Science Research Instrumentation Cigarette Smoke Generator For Inhalation and Analytic Studies

2 Contents Cigarette Smoke Generator Fully Automatic 3 Sensors 3 Cigarette Wheel 3 Technical Details 3 Cigarette Smoke Generator Semi Automatic 4 Dilution System 4 For use with Cell Culture Systems 4 Control and Recording Software 5 Output Monitor for Testing Purposes 5 Input Monitor 5 Data Acquisition 5 Partial List of Users 6 Ordering Information 7 2

3 Cigarette Smoke Generator Fully Automatic The Cigarette Smoke Generator (SMG) is an instrument for the automatic and continuous smoking of cigarettes for analytical and experimental investigations. Cigarette smoke generation takes place by the automatic loading, reloading, igniting, smoking and ejecting of cigarettes A cigarette magazine for up to 200 commercial or research cigarettes is provided The cigarette smoke is fed into the inhalation unit or analytical device via an internal pump Optional side stream smoke extraction is available Cigarette Wheel A cigarette wheel for up to 10 cigarettes is provided 20 cigarettes optional. Work can be carried out with up to 10 cigarettes, as required. Sensors Infrared sensors are located at different positions of the wheel to determine the condition of the cigarettes. The cigarettes are automatically loaded into the holder and ignited with an electric cigarette lighter During the experiment reloading is carried out automatically when a position that is to be occupied no longer contains a cigarette, thus ensuring a controlled supply of cigarettes to the wheel Several sensors are used to control the current status of the cigarettes in the wheel Various sensors are provided to monitor the butt and cigarette length as well as to check the loading of the wheel Automatic transmission of sensor data to the software is provided to trigger any function that may be necessary If a cigarette is missing from the holder then a loading mechanism will be triggered; if a cigarette was not properly lit or the defined butt length undercut the cigarette will be ejected Technical Details Standard configuration is for cigarettes with 84 mm length and 7.9 mm diameter (e.g. Kentucky Reference Cigarettes 1R4F, 2R4F, 3R4F). Other configurations are available upon request. One cigarette is drawn on, then the wheel turns (6 second intervals) and the next cigarette is drawn on Each cigarette is drawn on for a predefined time per minute with a preset puff volume Drawing duration: 1.5 up to 5 s, puff volume: 10 ml to 50/100 ml in 2 s Max. number of puffs per cigarette 3-20 The cigarette smoke generator is built in the scope of ISO 3308:2000 guidelines (where applicable) 3

4 Cigarette Smoke Generator Semi Automatic The 2-port Cigarette Smoke Generator is suited for studies with small exposure volumes. Mainstream and sidestream smoke can be extracted for research purposes Automatic puffing with semi-automatic igniting of the cigarettes For use with commercial cigarettes as well as research cigarettes of different sizes and diameters Cigarettes can be smoked according to protocols such as ISO 3308:2000, puff timing and volume can also be adjusted For Use with Cell Culture Systems The Cigarette Smoke Generator is applicable with cell culture systems enabling a reduced smoke concentration, crucial for cell culture exposure. Dilution System By using the 2-port Cigarette Smoke Generator each exposure unit can be provided with its own individual smoke-to-air ratio. For up to 4 exposure units can be connected to one cigarette smoke generator via a multi-stage dilution system Each exposure unit is equipped with a sensor for continuous CO monitoring values which exceed the entered threshold limits generate an alarm Time-saving and favorably priced procedure for tobacco smoke screening tests with tissue materials Uniform and controlled smoke exposure by the use of the proven control technique of the TSE inhalation systems Excess substance-air mixture is removed via an exhaust outlet and passes through a filter Exhaust analysis by a sample unit and purification using a downstream filter system Mixing and dilution units made of water-jacketed high quality laboratory glassware 4

5 Control and Recording Software The SMG software was developed to specify the smoking routine, trigger single actions and display status values. The main menu displays a graphical presentation of the wheel for status display, a table with test data and several submenus to define experiment parameters Setup specifications can be made for the entry of control parameters such as puffs per minute, puff volume, max. number of puffs per cigarette as well as descriptive text An additional menu for monitoring and testing the hardware inputs/ outputs is available A software function that rotates the cigarette wheel if no experiment is taking place is also provided Input Monitor The input monitor can be used to monitor which sensor is currently active or which function is currently being carried out without having to observe the smoke generator directly. Sensor signals from the internal functional units of the generator are passed on to the software via the control unit and displayed in the input monitor The input data is used to list the signals from the various sensors in the smoking chamber and to display them in the operating status The status indicator of the input monitor lights up green according to which function is currently active Output Monitor for Testing Purposes The output monitor shows control signals that are transmitted by the software to the Cigarette Smoke Generator via the control unit. A test mode is available to check the individual components such as cigarette loader and ejector, wheel rotation, etc., with the output monitor The status indicator lights up green according to which function is currently active The Input field Sec is used to specify the test time of the desired function Data Acquisition A log file is stored automatically in the default folder. It can be opened with a simple text editor. The file contains experimental data such as date/ time of the experiment start/ end, the entries in the setup fields, number of cigarettes used, number of puffs made, total volume of cigarette smoke, etc The log file can also be imported into spreadsheet programs; only a simple import routine needs to be carried out 5

6 Partial List of Users Chiesi Farmaceutici S.p.A., Parma, Italy KT&G Central Research Institute, Yuseong-gu, Daejeon, South Korea University of Minnesota, Minneapolis, MN, USA Institut für Umwelttoxikologie, Halle, Germany 6

7 Ordering Information Cat. No CSM-10/40 Description Cigarette Smoke Generator with 10 ports, 40 cigarette magazine for toxicological & analytical studies. automatic loading with special loading pattern for virtually continuous smoke generation automatic igniting automatic puffing automatic ejecting 1-10 cigarettes 40 cigarettes magazine (can be optional extended) 1 puff per minute for each cigarette 1 puff = 35 ml (other puff volumes optional) according to ISO 3308:2000 where applicable CSM-10/200 Cigarette Smoke Generator with 10 ports, 200 cigarette magazine for toxicological & analytical studies. automatic loading with special loading pattern for virtually continuous smoke generation automatic igniting automatic puffing automatic ejecting 1-10 cigarettes 200 cigarettes magazine (extension optional) various cigarette sizes 1 puff per minute for each cigarette puff volume adjustable ml (35 ml standard) or ml (70 ml standard), please specify according to ISO 3308:2000 where applicable side stream smoke hood and exhaust tubing control unit special interface PC system with TFT monitor and printer Windows and CSM software CSM-02 Cigarette Smoke Generator with 2 ports for toxicological & analytical studies. automatic puffing semi-automatic igniting manual loading and ejecting 1-2 cigarettes various cigarette sizes 1 puff per minute for each cigarette, adjustable puff volume adjustable ml (35 ml standard) or ml (70 ml standard), please specify according to ISO 3308:2000 where applicable control unit automatic loading and ejecting module optional (including magazine for 40 cigarettes) 7

8 Sophisticated Life Science Research Instrumentation TSE Systems offers additional modules to create a complete exposure system. Please find out more about: Head Nose Only Exposure Units Modular, stackable structure for different size conditioning units. Available for animal sizes from mouse to dog with directed-flow principle Whole Body Exposure Units Robust, user-friendly design for homogeneous test substance distribution. Different volumes available for variable animal sizes and test animal numbers Service & Warranty TSE Systems offers a Two (2) Years ALL- IN Premium Warranty with all new products, including: 24/7 technical hotline Remote maintenance and update function On-site visits upon necessity Free replacement parts Cell Culture Exposure System Ideally suited system for screening tests with tissue, using Transwell inserts of varying sizes. Extended viability of cultures by heating/humidification Nanoparticle Analysis TSE System offers instrumentation to analyze nanoparticle size and concentration, such as Condensation Particle Counter or Differential Mobility Analyzer After the expiry of the warranty period, TSE Systems offers comprehensive extensions of the warranty or economical maintenance and repair contracts to ensure the continued smooth running of your instruments. Please contact us for further details. Daco Inhalation Software Control and recording software for inhalation systems with multi-control mode for system extension. Advanced safety and GLP compliance features Sensors and Controllers TSE Systems supplies a number of environmental sensors and mass flow controllers specifically designed for each inhalation system Specifications subject to change without notice Copyright 2014 TSE Systems International Group - All rights reserved Info@TSE-Systems.com

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