Cellartis Hepatocyte Differentiation Kit User Manual
|
|
- Kelley Goodwin
- 6 years ago
- Views:
Transcription
1 Takara Bio Europe AB Cellartis Hepatocyte Differentiation Kit User Manual Cat. No. Y30050 (042216) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens backe 20, SE Göteborg, Sweden Europe Technical Support: tech-cellartis@takara-clontech.eu United States/Canada Asia Pacific Europe +46.(0) Japan +81.(0) Page 1 of 12
2 Table of Contents I. Introduction... 4 II. List of Components... 4 III. Additional Materials Required... 4 IV. General Considerations... 4 A. Storage and Handling... 4 V. Differentiation of Definitive Endoderm Cells to Hepatocytes... 5 A. Starting Material... 5 B. Media Volumes... 5 C. Medium Changes... 5 VI. Coating of Cell Culture Vessels... 6 VII. Day 0: Thawing and Seeding of Cellartis Definitive Endoderm Cells... 7 A. Preparation... 7 B. Thawing Cells... 7 VIII. Day 0: Passage and Seeding of Definitive Endoderm Cells... 8 A. Preparation... 8 B. Dissociation and Seeding Cells... 8 IX. Day 2 and 4: Medium Changes... 8 A. Preparations... 8 B. Medium Change Day 2 and X. Day 7 and 9: Medium Changes... 9 A. Preparations... 9 B. Medium Change Day C. Medium Change Day XI. Day 11 and Onwards: Medium Changes... 9 A. Preparations... 9 B. Medium Change XII. Images of Cells Differentiated using Cellartis Hepatocyte Differentiation Kit Appendix A. CYP Activity Assay A. Additional Material Required B. Preparation C. Activity Assay Page 2 of 12
3 Table of Figures Figure 1. Day 2 morphology of a human ips cell line differentiated using the Cellartis Hepatocyte Differentiation Kit Figure 2. Day 7 morphology of a human ips cell line differentiated using the Cellartis Hepatocyte Differentiation Kit Figure 3. Day 21 morphology of a human ips cell line differentiated using the Cellartis Hepatocyte Differentiation Kit Table of Tables Table I. Suggested schedule for Cellartis Hepatocyte Differentiation Kit Table II. CYP Substrate Cocktail Contact Us Customer Service/Ordering Technical Support tel: +33.(0) tel: +46.(0) fax: +33.(0) fax: +46.(0) web: web: orders@takara-clontech.eu tech-cellartis@takara-clontech.eu Notice to Purchaser This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, or household item, etc. This product may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from Takara Bio Europe AB. If you require licenses for other use, please contact us by phone at Your use of this product is also subject to compliance with any applicable licensing requirements as detailed in our catalogues, on our website at on the label or other documentation accompanying the goods. It is your responsibility to review, understand and adhere to any restrictions imposed by such statements. Takara and the Takara logo are trademarks of TAKARA HOLDINGS INC., Kyoto, Japan. Cellartis and DEF-CS are trademarks of Takara Bio Europe AB. All other trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. Takara Bio Europe AB is a Takara Bio company Takara Bio Europe AB This document has been reviewed and approved by the Takara Bio Europe AB Quality Assurance Department. Page 3 of 12
4 I. Introduction Cellartis Hepatocyte Differentiation Kit contains complete media and ready-to-use coating for the differentiation of definitive endoderm (DE) cells to hepatocytes in 2D culture. This product should only be handled by persons who have been trained in laboratory techniques and should only be used in accordance with the principles of good cell culture practice. Takara Bio Europe AB recommends the use of media and reagents according to this manual. Takara Bio Europe AB cannot guarantee correct technical feedback on customer cultures unless the below culture instructions have been followed. II. III. IV. List of Components Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050) o 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml) o 1 bottle Hepatocyte Progenitor Medium (2) (50 ml) o 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml) o 1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml) o 3 bottles Hepatocyte Maintenance Medium (4) (50 ml) o 1 tube Hepatocyte Coating (7.5 ml) Additional Materials Required The following materials are required but not supplied: Human definitive endoderm cells. Takara Clontech offers DE cells and kit for DE differentiation: o Cellartis Definitive Endoderm Cells (from ChiPSC18) (Cat. No. Y10040) o Cellartis Definitive Endoderm Differentiation Kit with DEF-CS Culture System (Cat. No. Y30035) Fetal bovine serum (FBS) or KnockOut Serum Replacement (KOSR)* PBS Dulbecco s w/o Ca 2+ & Mg 2+ (D-PBS / )* TrypLE Select Enzyme (1X) no phenol red* Cell culture vessels, tissue culture treated polystyrene surface General cell culture equipment used in cell culture laboratory * Needed if Cellartis Definitive Endoderm Differentiation Kit is used for DE differentiation General Considerations A. Storage and Handling Store all components of the Cellartis Hepatocyte Differentiation kit at 20 C; shelf life specified on product label. Thaw Hepatocyte Thawing and Seeding Medium (1) at 37 C ± 1 C and use the same day as thawing. Thaw Hepatocyte Progenitor Medium (2) at room temperature (RT, C), or overnight at 2 8 C, and use within 2 weeks. Thaw Hepatocyte Maturation Medium Base (3A) at RT and Hepatocyte Maturation Medium Supplement (3B) at 2 8 C. Use Hepatocyte Maturation Medium Base (3A) to prepare complete medium immediately after thawing. One vial of Hepatocyte Maturation Medium Supplement (3B) is enough for two medium changes and should be kept cold and used within 2 days of thawing. Once prepared by mixing 3A and 3B, Complete Hepatocyte Maturation Medium should be used fresh. Thaw Hepatocyte Maintenance Medium (4) at RT. Thawed Hepatocyte Maintenance Medium (4) should be used within three days or aliquoted and frozen the same day as thawing. Page 4 of 12
5 Thaw Hepatocyte Coating at RT and use directly when thawed. NOTES: Both Hepatocyte Maturation Medium Base (3A) and Hepatocyte Maintenance Medium (4) contain DMSO. Therefore, use nitrile gloves when preparing and changing medium and discard old medium in a closed container as hazardous waste. Both Hepatocyte Maturation Medium Base (3A) and Hepatocyte Maintenance Medium (4) are light sensitive; therefore avoid unnecessary exposure to light. V. Differentiation of Definitive Endoderm Cells to Hepatocytes Cellartis Hepatocyte Differentiation Kit consists of four different media and one coating compound. One kit is enough to differentiate DE cells to hepatocytes in 50 cm 2. The kit is optimized for differentiating cells in 24- or 96-well plates. Other formats can be used, but optimization may be required. All methods described in this user manual are for differentiation of DE cells to hepatocytes in 2D monolayer cultures on a total area of 50 cm 2. Table I shows a suggested workflow for hepatocyte differentiation. Coating can be performed on any of the days suggested, using a minimum coating time of 30 minutes in 37 C ± 1 C or overnight, prior seeding the cells. For examples of cell morphology during differentiation, please refer to Figure 1, Figure 2, and Figure 3. The images are just examples, and the morphology is not necessarily identical between experiments at the exact same time. The differentiated hepatocyte cells are ready to be used from day 14 after start of differentiation and can be maintained at least until day 25 if handled according to instructions,. The usage window for applications that requires high CYP activity is between day 16 and day 25. For optimal results, change the medium the day before starting an assay. NOTE: Always work under aseptic conditions. A. Starting Material Human Cellartis Definitive Endoderm Cells or DE cells derived using Cellartis Definitive Endoderm Differentiation Kit can be used as starting material. If using Cellartis Definitive Endoderm Cells, please see section VII for thawing and seeding instructions. If Cellartis Definitive Endoderm Differentiation Kit is used to derive the staring material, please see section VIII for dissociation and seeding of DE cells. B. Media Volumes 0.5 ml medium/cm 2 is used throughout the differentiation procedure. C. Medium Changes The following are general considerations for medium changes: The medium changes should be performed with care. It is recommended to use a pipette and aspirate roughly 75% of the medium in each well. Do not use a vacuum pump but pipette manually (use a multichannel pipette for 96-well plates). Aspirate the remaining medium in 4 12 wells at a time and add 0.5 ml/cm 2 (i.e. 1 ml/well for 24-well plates) to the empty wells. Repeat until the media is changed in each well. It is possible to temporarily aspirate the medium prior photo imaging. Try to take the images within 1 2 minutes after medium is aspirated. Then immediately add medium back to the well. Always discard any leftover warmed medium. Page 5 of 12
6 Table I. Suggested schedule for Cellartis Hepatocyte Differentiation Kit. Corresponding sections of this user manual are referenced in parentheses. Day Suggested Weekday Coating (VI) Thawing or Dissociation and Seeding (VII or VIII) Progenitor Medium (IX) Maturation Medium (X) Maintenance Medium (XI) Friday X Saturday X Sunday X 0 Monday X x 10 5 cells/cm ml/cm 2 1 Tuesday 2 Wednesday 0.5 ml/cm 2 3 Thursday 4 Friday 0.5 ml/cm 2 5 Saturday 6 Sunday 7 Monday 0.5 ml/cm 2 8 Tuesday 9 Wednesday 0.5 ml/cm 2 10 Thursday 11 Friday 0.5 ml/cm 2 12 Saturday 13 Sunday 14 Monday 0.5 ml/cm 2 15 Tuesday 16 Wednesday 0.5 ml/cm 2 17 Thursday 18 Friday 0.5 ml/cm 2 19 Saturday 20 Sunday 21 Monday 0.5 ml/cm 2 22 Tuesday 23 Wednesday 0.5 ml/cm 2 24 Thursday 25 Friday VI. Coating of Cell Culture Vessels 1. Thaw the frozen Hepatocyte Coating at RT. 2. Add the Hepatocyte Coating to the cell culture vessels (0.15 ml/cm 2 ). Make sure the entire surface is covered. 3. Incubate at 37 C ± 1 C for at least 30 min. 4. Remove excess Hepatocyte Coating from the cell culture vessels just before seeding. Do not let the surface dry out. Page 6 of 12
7 VII. Cellartis Hepatocyte Differentiation Kit User Manual Day 0: Thawing and Seeding of Cellartis Definitive Endoderm Cells Hepatocyte Thawing and Seeding Medium (1) and Hepatocyte Coating are used when thawing Cellartis Definitive Endoderm cells. (If starting with DE cells derived using Cellartis Definitive Endoderm Differentiation Kit, please see section VIII.) NOTE: Thawed Cellartis Definitive Endoderm cells are fragile. Avoid using a pipette for mixing the cell suspension if possible. Only use the pipette to seed the cells. Avoid centrifugation of the thawed cells if possible. One vial of Cellartis Definitive Endoderm containing 6 x 10 6 viable cells is enough for 50 cm 2 of culture area (i.e., one 24-well plate, one 12-well plate, 5 wells of a 6-well plate, or well plates). 0.5 ml cell suspension should be added per cm 2, giving a cell density of 1.25 x 10 5 cells/cm 2. If other formats than the ones listed above are to be used, please consider the following: o Frozen DE cells should be seeded at x 10 5 cells/cm 2. It is not necessary to count the cells upon thawing, but might be useful if other concentrations are desired. o Try to keep the medium volume to 0.5 ml medium/cm 2 when seeding the cells. It is important to thaw the cells rapidly. Ideally, the vial of cells should be kept in liquid nitrogen or on dry ice prior thawing. A. Preparation Thaw and warm the appropriate volume of Hepatocyte Thawing and Seeding Medium (1) to 37 C ± 1 C. Coat the appropriate number of cell culture vessels with Hepatocyte Coating according to above. B. Thawing Cells NOTE FOR YOUR PROTECTION: Wear a protective face mask and protective gloves. Use forceps when handling a frozen vial. Never hold the vial in your hand as the cryovial may explode due to rapid temperature changes. 1. Transfer, as quickly as possible, the frozen vials from liquid nitrogen to a 37 C ± 1 C water bath using forceps. 2. Thaw the cells by gently pushing the vial under the surface of the water, without swirling the vial. Do not submerge the cap of the vial in the water bath as this could contaminate the cells. 3. After approximately 1 min, check if the cell suspension is thawed by carefully turning the tube upside down. 4. Decontaminate the external surface with an appropriate disinfectant and place into the biological safety cabinet. 5. Once thawed, pour the cell suspension into 24 ml of 37 C ± 1 C Hepatocyte Thawing and Seeding Medium (1). Wash the vial with 1 ml of 37 C ± 1 C Hepatocyte Thawing and Seeding Medium (1) and add to the cells. 6. Mix by turning the tube with cell suspension carefully approximately 3 times. Do not mix by pipetting. 7. Optional: Count the viable cells using a haemocytometer. Take 50 µl of the cell suspension, add 5 µl trypan blue, count cells, and multiply the counted viable cells with 1.1 to get the concentration of viable cells. 8. Remove the Hepatocyte Coating from the cell culture vessels just prior to seeding the cells. 9. Using a 1-ml pipette, seed 0.5 ml cell suspension per cm 2. Mix the cell suspension by swirling or turning the tube, or use a pipette gently. 10. Place the cell culture vessels in an incubator at 37 C ± 1 C, 5% CO 2, 90% humidity and leave untouched overnight. The first medium changed is performed on day two after seeding (see section IX). Page 7 of 12
8 VIII. Day 0: Passage and Seeding of Definitive Endoderm Cells DE cells derived using Cellartis Definitive Endoderm Differentiation Kit can be dissociated at day seven after start of differentiation and used as starting material for hepatic differentiation. Hepatocyte Thawing and Seeding Medium (1) and Hepatocyte Coating are used when passaging and seeding DE cells. (If starting with Cellartis Definitive Endoderm cells, please see section VII.) A. Preparation Thaw and warm the appropriate volume of Hepatocyte Thawing and Seeding Medium (1) to 37 C ± 1 C. Warm TrypLE Select Enzyme and D-PBS / to 37 C ± 1 C. Prepare appropriate volume of 10% FBS or KOSR in D-PBS / and warm to RT. Coat the appropriate number of cell culture vessels with Hepatocyte Coating according to above. IX. B. Dissociation and Seeding Cells 1. Aspirate the medium in the culture vessels by using pipette or vacuum pump. 2. Wash the cell culture vessels with warm D-PBS / (0.1 ml/cm 2 ). 3. Remove the D-PBS in the culture units by using pipette or vacuum pump. 4. Add warm TrypLE Select Enzyme to the cell culture vessels (0.1 ml/cm 2 ). 5. Incubate the cell culture vessels in the incubator at 37 C ± 1 C and 5% CO 2 for 3-5 min until the cells have detached. 6. Transfer the cell suspension to a 50-ml tube. 7. Rinse the cell culture vessels with 10 % FBS or KOSR in D-PBS / (0.1 ml/cm 2 ) and transfer to the 50-ml tube to achieve a 1:1 dilution of the cell suspension. 8. Count the cells. 9. Transfer a volume of the cell suspension corresponding to 6.5 x 10 6 cells to a centrifugation tube. 10. Centrifuge the cell suspension at 300 x g for 5 min at RT and resuspend the cell pellet in 25 ml Hepatocyte Thawing and Seeding Medium (1). 11. Using a 1-ml pipette, seed 0.5 ml cell suspension per cm 2, corresponding to x 10 5 cells/cm 2. It is crucial to seed more than 1.0 x 10 5 cells/cm 2 in order to get a successful hepatic differentiation. 12. Place the cell culture vessels in an incubator at 37 C ± 1 C, 5% CO 2, 90% humidity and leave untouched overnight. First medium change is performed day two after seeding, see section IX. Day 2 and 4: Medium Changes On day two and four after thawing, Hepatocyte Progenitor Medium (2) is used for medium changes. A. Preparations Thaw the bottle with Hepatocyte Progenitor Medium (2) at RT, or overnight in 2 8 C. Transfer 25 ml to a flask or tube for use on day 2 and warm to 37 C ± 1 C. Keep the remaining 25 ml of Hepatocyte Progenitor Medium (2) at 2 8 C, for medium change on day 4. B. Medium Change Day 2 and 4 1. Warm appropriate volume of Hepatocyte Progenitor Medium (2) to 37 C ± 1 C. 2. Aspirate the medium from the cell culture vessels according to section V.C and discard. 3. Carefully add warm Hepatocyte Progenitor Medium (2) to the cell culture plate, 0.5 ml/cm Place the cells in an incubator at 37 C ± 1 C, 5% CO 2, 90 % humidity. Page 8 of 12
9 X. Day 7 and 9: Medium Changes On days seven and nine after thawing, Hepatocyte Maturation Medium Base (3A) and Hepatocyte Maturation Medium Supplement (3B) are used for medium changes. NOTES: Hepatocyte Maturation Medium Base (3A) is light sensitive, therefore avoid unnecessary exposure to light. Hepatocyte Maturation Medium Base (3A) contains DMSO. Therefore, use nitrile gloves when preparing and changing medium and discard old medium in a closed container as hazardous waste. Keep the Hepatocyte Maturation Medium Supplement (3B) cold until just prior to use. Only half the volume of the supplement should be used on day 7. Therefore, keep the remaining Hepatocyte Maturation Medium Supplement (3B) at 2 8 C for final use on day 9. Be careful when the medium is changed. From day 9 and onwards, we recommend leaving a small volume in the well when aspirating the medium prior to adding fresh medium. A. Preparations Thaw Hepatocyte Maturation Medium Base (3A) at RT or overnight at 2 8 C. Thaw Hepatocyte Maturation Medium Supplement (3B) at 2 8 C. One vial contains supplement for both days 7 and 9, always keep cold and store the volume for day 9 at 2 8 C. Warm 24 ml of Hepatocyte Maturation Medium Base (3A) no warmer than RT. Add 1.3 ml Maturation Medium Supplement per 24 ml Hepatocyte Maturation Medium Base (3A) to get complete Hepatocyte Maturation Medium. Mix gently and use immediately. B. Medium Change Day 7 1. Remove the medium from the cell culture vessels according to section V.C and discard. 2. Carefully add RT Hepatocyte Maturation Medium to the cell culture plate, 0.5 ml/cm Place the cells in an incubator at 37 C ±1 C, 5% CO 2, 90% humidity. XI. C. Medium Change Day 9 1. On day 9, some components of the Hepatocyte Maturation Medium from day 7 has gelatinised on top of the cell layer. Be careful when medium is changed; aspirate carefully as described in section V.C. We recommended leaving a small volume is left in the well when aspirating the medium prior to adding fresh medium. 2. Carefully add RT Hepatocyte Maturation Medium to the cell culture plate, 0.5 ml/cm Place the cells in an incubator at 37 C ± 1 C, 5% CO 2, 90% humidity. Day 11 and Onwards: Medium Changes From day 11 and onwards Hepatocyte Maintenance Medium (4) is used for medium changes. The recommended days for medium changes are Mondays, Wednesdays, and Fridays. No medium change is necessary during the weekend. NOTES: Hepatocyte Maintenance Medium (4) is light sensitive, therefore avoid unnecessary exposure to light. Hepatocyte Maintenance Medium (4) contains DMSO. Therefore, use nitrile gloves when preparing and changing medium and discard old medium in a closed container as hazardous waste. Be careful when the medium is changed. We recommend leaving a small volume in the well when aspirating the medium prior to adding fresh medium. A. Preparations Thaw Hepatocyte Maintenance Medium (4) in RT. Hepatocyte Maintenance Medium (4) can be in kept 2 8 C for up to three days. One bottle of Hepatocyte Maintenance Medium (4) is enough for two medium changes. Page 9 of 12
10 B. Medium Change 1. Warm appropriate volume of Hepatocyte Maturation Medium to RT. 2. Aspirate the medium from the cell culture vessels according to section V.C and discard. 3. Carefully add Hepatocyte Maturation Medium to the cell culture plate, 0.5 ml/cm Place the cells in an incubator at 37 C ± 1 C, 5% CO2, 90% humidity. The differentiated hepatocyte cells are ready to be used in your preferred application beginning on day 14 after the start of DE differentiation. The hepatocytes can be maintained until at least day 25 if handled according to these instructions. Applications that require high CYP activity should be performed from day 16. For optimal results, change the medium the day before starting an assay. NOTE: The Hepatocyte Maturation Medium contains matrix components which might influence protein measurements and immunocytochemistry imaging. XII. Images of Cells Differentiated using Cellartis Hepatocyte Differentiation Kit Figures 1 3 are examples of morphology of definitive endoderm cells differentiated using Cellartis Hepatocyte Differentiation Kit. The morphology may vary depending on the cell line used and cell density. 4X (Scale bar corresponds to 250 µm.) 10X (Scale bar corresponds to 100 µm.) Figure 1. Day 2 morphology of a human ips cell line differentiated using the Cellartis Hepatocyte Differentiation Kit. Scale bar corresponds to 250 microns (Panel A) and 100 microns (Panel B). 4X (Scale bar corresponds to 250 µm.) 10X (Scale bar corresponds to 100 µm.) Figure 2. Day 7 morphology of a human ips cell line differentiated using the Cellartis Hepatocyte Differentiation Kit. Scale bar corresponds to 250 microns (Panel A) and 100 microns (Panel B). Page 10 of 12
11 10X (Scale bar corresponds to 100 µm.) 20X (Scale bar corresponds to 50 µm.) Figure 3. Day 21 morphology of a human ips cell line differentiated using the Cellartis Hepatocyte Differentiation Kit. Scale bar corresponds to 100 microns (Panel A) and 50 microns (Panel B). Page 11 of 12
12 Appendix A. CYP Activity Assay After 16 days in culture, the differentiated hepatocytes can be used in Cytochrome P450 (CYP) activity assays. Samples can be analysed using LC/MS to measure the formation of the following specific metabolites: acetaminophen (CYP1A), 4-OH-Diclofenac (CYP2C9), 4-OH-Mephenytoin (CYP2C19), OH-Bufuralol (CYP2D6) and 1-OH-Midazolam (CYP3A). A. Additional Material Required Williams Medium E (WME), w/o L-glutamine and phenol red (Sigma-Aldrich, Cat. No. W1878) 4,6-Diamidine-2 -phenylindole dihydrochloride (DAPI) HEPES Solution, 1 M L-Glutamine Solution, 200 mm Penicillin/Streptomycin (PEST) (10,000 units/ml of penicillin and 10,000 µg/ml of streptomycin) Probe Substrate Cocktail: o Phenacetin (Sigma-Aldrich, Cat. No ) o Bupropion (Sigma-Aldrich, Cat. No B102) o Mephenyotin (Santa Cruz, Cat. No. sc a) o Diclofenac (Sigma-Aldrich, Cat. No. D6899) o Bufuralol (Becton Dickinson, Cat. No ) o Midazolam (Larodan, Cat. No. MID-111-HC) B. Preparation Prepare a stock solution for the CYP substrate cocktail. In the assay, use the final concentrations listed in Table II. Prepare medium to be used as washing medium and as basal medium for the CYP activity medium by adding 0.1% PEST to WME medium. Warm the appropriate volume of WME (0.1% PEST) for washing to 37 C ± 1 C. Prepare and warm CYP activity assay medium: WME (0.1% PEST), 25 mm HEPES, 2 mm L- Glutamine. Add the CYP substrate cocktail just prior to use. Table II. CYP Substrate Cocktail CYP Substrate Final Assay Concentration 1A Phenacetin 10 µm 2B6 Bupropion 10 µm 2C19 Mephenytoin 50 µm 2C9 Diclofenac 10 µm 2D6 Bufuralol 10 µm 3A Midazolam 5 µm C. Activity Assay 1. Wash the differentiated hepatocytes twice very gently with 0.5 ml/cm 2 warm WME (0.1% PEST). 2. Add 110 µl/cm 2 warm CYP activity assay medium to the cells. 3. Incubate for 2 hours at 37 C ± 1 C, 5% CO 2, 90% humidity. 4. Collect 100 µl supernatant and store at 80 C until LC/MS analysis. 5. It is recommended that the cells are fixed after treatment and stained with DAPI. Using a fluorescence microscope, count the number of nuclei/field, then calculate the number of nuclei/well. 6. Normalize the metabolite concentrations measured by LC/MS to the amount of nuclei per well and the assay duration (120 min). NOTE: Hepatocytes do often contain two or more nuclei/cell, therefore do not normalize to number of cells/well. If the cells have been evenly seeded on day 0, there will be only minor variations between wells. Page 12 of 12
Cellartis Hepatocyte Diff Kit User Manual
Takara Bio Europe AB Cellartis Hepatocyte Diff Kit User Manual Cat. No. Y30050 (061715) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens backe 20, SE-413 46 Göteborg, Sweden Europe Technical Support:
More informationCellartis Enhanced hips-hep v2 Kits User Manual
Takara Bio Europe AB Cellartis Enhanced hips-hep v2 Kits User Manual Cat. Nos. Y10133, Y10134 & Y10135 (082217) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens backe 20, SE-413 46 Göteborg, Sweden
More informationCellartis DEF-CS Culture System User Manual
Takara Bio Europe AB Cellartis DEF-CS Culture System Cat. No. Y30010 and Y30020 (092717) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens backe 20, SE-413 46 Göteborg, Sweden Europe Technical
More informationHuman ipsc-derived Ventricular Cardiomyocytes. Protocol version 3.1
Human ipsc-derived Ventricular Cardiomyocytes Protocol version 3.1 Protocol version 3.1 Table of Contents Product Information 2 Recommendations 2 Preparing Cardiomyocyte Maintenance Medium 3 Cardiomyocyte
More informationPluricyte Cardiomyocytes
Pluricyte Cardiomyocytes Manual Version 2.1 / March 2018 Contents 1. Introduction 2 2. Equipment, Materials and Reagents 3 3. Methods 4 3.1 Coating of tissue culture plates 4 3.2 Thawing Pluricyte Cardiomyocytes
More informationProduct Use HPSC-CC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
HPSC-derived Cardiomyocyte Cells (HPSC-CC) Catalog #6240 Cell Specification Human primary cardiomyocytes and cardiac tissue are superior modeling systems for heart disease studies, drug discovery and toxicity
More informationRODENT Hepatocytes Care Manual
RODENT Hepatocytes Care Manual INSTRUCTION MANUAL ZBM0054.02 SHIPPING CONDITIONS Rodent Hepatocytes cryopreserved Orders are delivered via Federal Express courier. All US and Canada orders are shipped
More informationCryopreserved HepaRG Cells and Media Supplements
Cryopreserved HepaRG Cells and Media Supplements Catalog No. MMHPR116 Catalog No. MMADD621 Catalog No. MMADD631 Catalog No. MMADD641 Catalog No. MMADD651 Catalog No. MMADD671 FOR RESEARCH USE ONLY Not
More informationTo place an order, please visit lifelinecelltech.com or call customer service at
Human Melanocyte Cells Instruction Manual HEMn Human Epidermal Melanocytes, Neonatal HEMn-HP Human Epidermal Melanocytes, Neonatal-Highly Pigmented HEMa Human Epidermal Melanocytes, Adult HEMa-HP Human
More informationMouse Hepatic Progenitor Organoid Culture: Supplementary Protocols
TECHNICAL BULLETIN Mouse Hepatic Progenitor Organoid Culture: The following are supplementary protocols for the culture of hepatic organoids with HepatiCult Organoid Growth Medium (Mouse) (Catalog #06030).
More informationNINDS Repository Human Induced Pluripotent Stem Cell (ipsc) Handling Protocols (Matrigel and mtesr Media)
General Guidelines for Handling Human ipsc cells ipsc are cryopreserved in plastic cryovials and shipped on dry ice. If storing the ipsc before thawing, store in liquid nitrogen vapor. Storage directly
More informationAssessment of pro-arrhythmic effects using Pluricyte Cardiomyocytes. on the ACEA xcelligence RTCA CardioECR
Assessment of pro-arrhythmic effects using Pluricyte Cardiomyocytes on the ACEA xcelligence RTCA CardioECR User Guide Version 3.0 / March 2018 Contents 1. Introduction 2 2. Workflow 3 3. Important recommendations
More informationFor research or further manufacturing use only. Not for injection or diagnostic procedures.
PRIME-XV T cell Expansion XSFM PRIME-XV T Cell Expansion XSFM is a xeno-free, serum-free medium optimized for the activation and expansion of human T lymphocytes. This medium contains gentamicin and requires
More informationHuman Induced Plutipotent Stem Cell (ipsc) Handling Protocols: Matrigel and mtesr/e8 Media
General Guidelines for Handling Human ipsc cells ipsc are cryopreserved in plastic cryovials and shipped on dry ice. If storing the ipsc before thawing, store in liquid nitrogen vapor. Storage directly
More informationStriatal Neuron Medium Kit
Striatal Neuron Medium Kit Product Information What are included in the Striatal Neuron Medium Kit (ax0333): 2x 250 ml Striatal Neuron Basal Medium (Store at 4 o C upon receipt) 2x 7.5 ml Striatal Neuron
More informationPluricyte Cardiomyocytes. using the Multiwell MEA System from Multi Channel Systems
Pluricyte Cardiomyocytes using the Multiwell MEA System from Multi Channel Systems User Guide Version 3.1 / March 2018 Contents 1. Introduction 2 2. Workflow 3 3. Important Recommendations 4 4. Equipment,
More informationTECHNICAL BULLETIN. Primary Human Hepatocytes LifeNet Health
Primary Human Hepatocytes LifeNet Health Catalog Number: MTOXH1000 - Cryoplateable Primary Human Hepatocytes MTOXH1001 - Cryopreserved Primary Human Hepatocytes Metabolism Qualified Suspension Grade MTOXH1002
More information91154 PRIME-XV T Cell CDM Liquid 1 L Additional package sizes are available at request
PRIME-XV T CELL CDM PRIME-XV T Cell CDM is a ready-to-use chemically-defined, animal component-free medium. It is optimized and designed for the culture of T cells of human origin and recommended for use
More informationThawing MEFs (Mouse Embryonic Fibroblasts (MEFs)
1 FEEDER CULTURES The function of feeder cultures is to support the undifferentiated growth of hpsc. Typically primary fibroblasts are used for this purpose. We prepare our mouse feeder cells from ICR
More informationHuman Gingival Epithelial Cells. Protocol version 1.0
Human Gingival Epithelial Cells Protocol version 1.0 Protocol version 1.0 Table of Contents Human Gingival Epithelial Cells 2 Recommendations 2 Thawing and Plating 2 Passaging 3 Usage Statement 3 1 Human
More informationProtocol for Thawing and Use of Plateable and Suspension Cryopreserved Hepatocytes
Protocol for Thawing and Use of Plateable and Suspension Cryopreserved Hepatocytes Introduction This protocol covers the thawing and prep of cryopreserved hepatocytes for their subsequent use in applications
More informationHuman Keratinocytes. Protocol version 1.0
Human Keratinocytes Protocol version 1.0 Protocol version 1.0 Table of Contents Human Keratinocytes 2 Recommendations 2 Thawing and Plating 2 Passaging 3 Usage Statement 3 1 Human Keratinocytes Human
More informationCorning Cryopreserved HepatoCells Instructions for Use
Corning Cryopreserved HepatoCells Instructions for Use Corning cryopreserved HepatoCells are derived from single-donor human hepatocytes using proprietary technology for immortalizing primary cells. HepatoCells
More informationHuman ipsc-derived Microglial Precursors
Human ipsc-derived Microglial Precursors Product Information Catalog. No. Product Name Format Storage on Arrival ax1666 Human ipsc-derived Microglial Precursors 3 x 10 6 viable cells Transfer cells to
More informationHuman Dermal Microvascular Endothelial Cells. Protocol version 1.0
Human Dermal Microvascular Endothelial Cells Protocol version 1.0 Protocol version 1.0 Table of Contents Human Airway Epithelial Cells 2 Recommendations 2 Thawing and Plating 2 Passaging 3 Usage Statement
More informationFollicle Dermal Papilla Cells
Follicle Dermal Papilla Cells Instruction Manual Product Size Catalog Number Human Follicle Dermal Papilla Cells (HFDPC) 500,000 cryopreserved cells 500,000 proliferating cells C-12071 C-12072 Product
More informationCryopreserved Human Hepatocytes (Cat # HP-F)
Certificate of Analysis Cryopreserved Human Hepatocytes (Cat # HP-F) This CoA represents a single Lot specific batch of cells. For details of current Lots available please contact us. Lot#: H0434 Gender
More informationReceiving Cryopreserved Human Hepatocytes
Receiving Cryopreserved Human Hepatocytes TECHNICAL BULLETIN Purpose The purpose of this bulletin is to provide instructions and recommendations for the proper receiving and transfer of cryopreserved human
More informationHuman Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) Introduction Kit Components Cat. # # of vials Reagent Quantity Storage
Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) Catalog #5901 Introduction Human pluripotent stem cells (hpsc), including embryonic stem cells (ESC) and induced pluripotent stem
More informationaxion Protocol Cell Culture on Microelectrode Arrays Cell Type: QBM Cell Science - E14,15 Embryonic C57 Mouse Cortical Neurons BioSystems v. 1.
axion BioSystems Cell Culture on Microelectrode Arrays Cell Type: QBM Cell Science - E14,15 Embryonic C57 Mouse Cortical Neurons Protocol v. 1.1 Trademarks Axion BioSystems, Inc. and the logo are trademarks
More informationFollicle Dermal Papilla Cell
Follicle Dermal Papilla Cell Instruction Manual Product Size Catalog Number Human Follicle Dermal Papilla Cells (HFDPC) 500,000 cryopreserved cells 500,000 proliferating cells C-12071 C-12072 Product Description
More informationMacrophage Generation Media DXF
Macrophage Generation Media DXF Instruction Manual Macrophage Generation Media DXF Product Size Catalog Number M1-Macrophage Generation Medium DXF 250 ml C-28055 M2-Macrophage Generation Medium DXF 250
More informationProduct Size Catalog Number. 500,000 proliferating cells. cryopreserved cells that have been thawed and cultured for three days at PromoCell.
Cardiac Myocytes Instruction Manual Product Size Catalog Number Human Cardiac Myocytes (HCM) 500,000 cryopreserved cells 500,000 proliferating cells C-12810 C-12811 Product Description Cardiac muscle,
More informationHuman Umbilical Vein Endothelial Cell Manual
Human Umbilical Vein Endothelial Cell Manual INSTRUCTION MANUAL ZBM0079.03 SHIPPING CONDITIONS Human Umbilical Vein Endothelial Cells, cryopreserved Cryopreserved cells are shipped on dry ice and should
More informationnachr α 4 β 2 CHO Cell Line
B SYS GmbH nachr α 4 β 2 CHO Cell Line Cell Culture Conditions B SYS GmbH B SYS GmbH nachr α 4 β 2 CHO Page 2 TABLE OF CONTENTS 1 BACKGROUND...3 1.1 Human Nicotinic Acetylcholine Receptors...3 1.2 B SYS
More informationWiCell Neural Stem Cell Protocols
WiCell Neural Stem Cell Protocols 2008 WiCell Preface This booklet of protocols is intended to serve as a primer for culturing neural stem cells in NSC culture medium. These protocols are representative
More informationHuman Mammary Luminal Epithelial Cells. Manual
Human Mammary Luminal Epithelial Cell Manual INSTRUCTION MANUAL SHIPPING CONDITIONS ZBM0071.00 Human Mammary Luminal Epithelial Cells Orders are delivered via Federal Express courier. All US and Canada
More informationMeasuring Cardiac Activity:
icell Cardiomyocytes 2 Application Protocol Measuring Cardiac Activity: Impedance and Extracellular Field Potential Detection with CardioExcyte 96 System Introduction icell Cardiomyocytes 2, human cardiomyocytes
More informationBacPAK Baculovirus Rapid Titer Kit
BacPAK Baculovirus Rapid Titer Kit United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Cat. No. 631406 PT3153-1 (072213) Clontech Laboratories,
More informationPlasma Membrane Protein Extraction Kit
ab65400 Plasma Membrane Protein Extraction Kit Instructions for Use For the rapid and sensitive extraction and purification of Plasma Membrane proteins from cultured cells and tissue samples. This product
More informationaxion Protocol Cell Culture on Microelectrode Arrays Cell Type: GE Healthcare - Cytiva TM Plus Cardiomyocytes BioSystems v. 1.0
axion BioSystems Cell Culture on Microelectrode Arrays Cell Type: GE Healthcare - Cytiva TM Plus Cardiomyocytes Protocol v. 1.0 Trademarks Axion BioSystems, Inc. and the logo are trademarks of Axion BioSystems,
More informationProtocol for Thawing Cryopreserved Hepatocytes
cell and tissue-based products Protocol for Thawing Cryopreserved Hepatocytes Product Instruction The following procedure may be carried out in a biosafety containment hood to reduce the risk of contamination
More informationTRACP & ALP Assay Kit
Cat. # MK301 For Research Use TRACP & ALP Assay Kit Product Manual Table of Contents I. Description...3 II. III. IV. Introduction...3 Components...4 Materials Required but not Provided...4 V. Storage...4
More informationEndothelial Cells (Microvascular)
Endothelial Cells (Microvascular) Instruction Manual Product Size Catalog Number Human Dermal Microvascular Endothelial Cells (HDMEC) juvenile foreskin Human Dermal Microvascular Endothelial Cells (HDMEC)
More informationEndothelial Cells (Large Vessels)
Endothelial Cells (Large Vessels) Instruction Manual Product Size Catalog Number Endothelial Cells (HUVEC) single donor Endothelial Cells (HUVEC) pooled Endothelial Cells (HUVEC) isolated in Growth Medium
More informationHuman Keratinocyte Manual
INSTRUCTION MANUAL SHIPPING CONDITIONS Human Keratinocyte Cells Human Keratinocyte Manual ZBM0032.09 Orders are delivered via Federal Express courier. All US and Canada orders are shipped via Federal Express
More informationINSTRUCTIONS Pierce Primary Cardiomyocyte Isolation Kit
INSTRUCTIONS Pierce Primary Cardiomyocyte Isolation Kit 88281 Number Description 88281 Pierce Primary Cardiomyocyte Isolation Kit, contains sufficient reagents to isolate cardiomyocytes from 50 neonatal
More informationHuman Umbilical Cord Blood CD34 + /133+ Progenitor Cell Care Manual
Human Umbilical Cord Blood CD34 + /133+ Progenitor Cell Care Manual INSTRUCTION MANUAL ZBM0065.04 SHIPPING CONDITIONS Human Umbilical Cord Blood CD34+/133+ Progenitor Cells, cryopreserved Cryopreserved
More informationCHO α 1 β 2 γ 2 GABAA Cell Line
B SYS GmbH CHO α 1 β 2 γ 2 GABAA Cell Line Specification Sheet B SYS GmbH B SYS GmbH CHO α 1 β 2 γ 2 Cells Page 2 TABLE OF CONTENTS 1 BACKGROUND...3 1.1 THE PHARMACOLOGICAL DISTINCTION OF GABA A RECEPTOR
More informationTECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C
Phospho-Stat3 (ptyr 705 ) and pan-stat3 ELISA Kit for detection of human, mouse, or rat phospho-stat3 (ptyr 705 ) and pan-stat3 in cell and tissue lysates Catalog Number RAB0447 Storage Temperature 20
More informationNF-κB p65 (Phospho-Thr254)
Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 NF-κB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02015 Please read the provided manual
More informationMeasuring Cardiac Activity:
icell Cardiomyocytes 2 Application Protocol Measuring Cardiac Activity: Impedance Detection with xcelligence RTCA Cardio System Introduction icell Cardiomyocytes 2, human cardiomyocytes derived from induced
More informationE.Z.N.A. SQ Blood DNA Kit II. Table of Contents
E.Z.N.A. SQ Blood DNA Kit II Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Blood Storage and DNA Yield...4 Preparing Reagents...5 100-500 μl Whole Blood Protocol...6
More informationHuman CD4+T Cell Care Manual
Human CD4+T Cell Care Manual INSTRUCTION MANUAL ZBM0067.04 SHIPPING CONDITIONS Human CD4+T Cells, cryopreserved Cryopreserved human CD4+T cells are shipped on dry ice and should be stored in liquid nitrogen
More informationProduct Size Catalog Number
Epithelial Cells Instruction Manual Product Size Catalog Number Human Nasal Epithelial Cells (HNEpC) Human Tracheal Epithelial Cells (HTEpC) Human Bronchial Epithelial Cells (HBEpC) Human Small Airway
More informationThawing. 10,000-15,000 cells per cm² 10,000-15,000 cells per cm² C ,000-15,000 cells per cm². Product Size Catalog Number
5 Instruction Manual Epithelial Cells Specifications Product Recommended Culture Media* Plating Density Passage after Thawing Marker Population Doublings Human Nasal Epithelial Cells (HNEpC) Human Tracheal
More informationaxion Protocol Cell Culture on Microelectrode Arrays Cell Type: Cryopreserved Human ipsc-derived Neurons BioSystems v. 1.1
axion BioSystems Cell Culture on Microelectrode Arrays Cell Type: Cryopreserved Human ipsc-derived Neurons Protocol v. 1.1 Trademarks Axion BioSystems, Inc. and the logo are trademarks of Axion BioSystems,
More informationL6 GLUT4myc Cell Growth Protocol
L6 GLUT4myc Cell Growth Protocol Background: Parental L6 cells selected for high fusion (2, 3) were stably transfected with a rat GLUT4 cdna carrying a myc epitope (recognized by the commercially available
More informationab ATP Synthase Enzyme Activity Microplate Assay Kit
ab109714 ATP Synthase Enzyme Activity Microplate Assay Kit Instructions for Use For the quantitative measurement of ATP Synthase activity in samples from Human, Rat and Cow This product is for research
More informationAlpha-Tubulin Housekeeping 10,000 tests
Headquarters & Europe Office Cisbio Bioassays Phone: +33 (0)4 66 79 67 05 Fax: +33 (0)4 66 79 19 20 bioassays@cisbio.com cisbio_dd_pi_64atubpeh USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax: +1
More informationab65311 Cytochrome c Releasing Apoptosis Assay Kit
ab65311 Cytochrome c Releasing Apoptosis Assay Kit Instructions for Use For the rapid, sensitive and accurate detection of Cytochrome c translocation from Mitochondria into Cytosol during Apoptosis in
More informationCONSENSUS PROTOCOL FOR PBMC CRYOPRESERVATION AND THAWING Version 3.0 Revision dated 1/8/02 (Changes are in Red Type)
CONSENSUS PROTOCOL FOR PBMC CRYOPRESERVATION AND THAWING Version 3.0 Revision dated 1/8/02 (Changes are in Red Type) 1. EQUIPMENT/SUPPLIES/REAGENTS 1.1 Gloves (latex, vinyl, nitrile) 1.2 Lab coat or protective
More informationImmature organoids appear after hours.
THE ESSENTIALS OF LIFE SCIENCE RESEARCH GLOBALLY DELIVERED Allison Ruchinskas, B.S., and James Clinton, Ph.D. ATCC Cell Systems, Gaithersburg, MD INTRODUCTION Figure 1. Mouse small intestinal organoid
More informationab Lysosome/Cytotoxicity Dual Staining Kit
ab133078 Lysosome/Cytotoxicity Dual Staining Kit Instructions for Use For studying lysosome function at the cellular level. This product is for research use only and is not intended for diagnostic use.
More informationTECHNICAL BULLETIN. Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates
Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates Catalog Number RAB0011 Storage Temperature 20 C TECHNICAL BULLETIN Product Description
More informationab MitoTox Complex I OXPHOS Activity Microplate Assay
ab109903 MitoTox Complex I OXPHOS Activity Microplate Assay Instructions for Use For the quantitative measurement of Complex I activity in Bovine samples This product is for research use only and is not
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More informationab Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric)
Version 10b Last updated 19 December 2018 ab118970 Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric) For the measurement of Lipid Peroxidation in plasma, cell culture and tissue extracts.
More informationab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric)
Version 10 Last updated 19 December 2017 ab65336 Triglyceride Quantification Assay Kit (Colorimetric/ Fluorometric) For the measurement of triglycerides in various samples. This product is for research
More informationab HIV-1 Protease Inhibitor Screening Kit (Fluorometric)
Version 1 Last updated 2 March 2017 ab211106 HIV-1 Protease Inhibitor Screening Kit (Fluorometric) For the rapid, sensitive and accurate screening of potential HIV-1 Protease inhibitors. This product is
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More informationLipid Droplets Fluorescence Assay Kit
Lipid Droplets Fluorescence Assay Kit Item No. 500001 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION
More informationRat Primary Pre-adipocytes Culture Kit
Primary Cell Co., Ltd Rat Primary Pre-adipocytes Culture Kit Primary Cells from rat mesenteric, epididymal, and subcutaneous adipose tissues. Catalog # PMC-VAC01-COS, PMC-EAC01-COS, PMC-SAC01-COS Notice
More informationNuclear Extraction Kit
Nuclear Extraction Kit Item No. 10009277 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION 3 Materials
More informationInstructions. Fuse-It-mRNA easy. Shipping and Storage. Overview. Kit Contents. Specifications. Note: Important Guidelines
Membrane fusion is a highly efficient method for transfecting various molecules and particles into mammalian cells, even into sensitive and primary cells. The Fuse-It reagents are cargo-specific liposomal
More informationBaculoELISA Titer Kit User Manual
User Manual Cat. No. 631412 PT3953-1 (PR752243) Published 5 May 2007 Table of Contents I. Introduction...3 II. III. IV. List of Components...4 Additional Materials Required...5 BaculoELISA Titer Kit Protocol
More informationNuclear Extraction Kit
Nuclear Extraction Kit Catalog Number KA1346 50 assays Version: 07 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Principle of the Assay... 3 General Information... 4
More informationPROTOCOL: OPTIMIZATION OF LENTIVIRAL TRANSDUCTION USING SPINFECTION
Last Modified: April 2018 Last Review: October 2018 PROTOCOL: OPTIMIZATION OF LENTIVIRAL TRANSDUCTION USING SPINFECTION Table of Contents 1. Brief Description 1 2. Materials and Reagents.1 3. Optimization
More informationLumino Firefly Luciferase Assay
G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Lumino Firefly Luciferase Assay (Cat. # 786 1267, 786 1268) think proteins! think G-Biosciences
More informationMidi Plant Genomic DNA Purification Kit
Midi Plant Genomic DNA Purification Kit Cat #:DP022MD/ DP022MD-50 Size:10/50 reactions Store at RT For research use only 1 Description: The Midi Plant Genomic DNA Purification Kit provides a rapid, simple
More informationLenti-X p24 Rapid Titer Kit User Manual
User Manual Lenti-X p24 Rapid Titer Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc. A Takara
More informationHuman Utrophin 500 tests
Headquarters & Europe Office Cisbio Bioassays Phone: +33 (0)4 66 79 67 05 Fax: +33 (0)4 66 79 19 20 bioassays@cisbio.com USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax: +1 781 687 1500 htrfinfo@cisbio.us
More informationab Membrane Fractionation Kit Instructions for Use For the rapid and simple separation of membrane, cytosolic and nuclear cellular fractions.
ab139409 Membrane Fractionation Kit Instructions for Use For the rapid and simple separation of membrane, cytosolic and nuclear cellular fractions. This product is for research use only and is not intended
More informationTrident Membrane Protein Extraction Kit
Cat. No. Size Shelf life GTX16373 5/ 20 tests 12 months at the appropriate storage temperatures (see below) Contents Component Storage Amount for 5 tests Amount for 20 tests Buffer A -20 o C 2.5 ml 10
More informationab Membrane fluidity kit Instructions for Use For the detection of membrane fluidity in cells
ab189819 Membrane fluidity kit Instructions for Use For the detection of membrane fluidity in cells This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated
More informationLenti-X p24 Rapid Titer Kit User Manual
Lenti-X p24 Rapid Titer Kit User Manual Cat. No. 632200 (111616) 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: techus@takarabio.com United States/Canada 800.662.2566 Asia
More informationab LDL Uptake Assay Kit (Fluorometric)
ab204716 LDL Uptake Assay Kit (Fluorometric) Instructions for Use For rapid, sensitive and accurate measuring of LDL uptake. This product is for research use only and is not intended for diagnostic use.
More informationCryoStor CS2, CS5 and CS10 FREEZE MEDIA
CryoStor CS2, CS5 and CS10 FREEZE MEDIA Pre-Formulated Serum-Free Protein-Free cgmp Manufactured FDA Master File Sterility, Endotoxin, and Cell-Based Release Testing CryoStor, a series of cell-specific,
More informationRayBio Human Phosphotyrosine BTK ELISA Kit
RayBio Human Phosphotyrosine BTK ELISA Kit Catalog #: PEL-BTK-Y User Manual Last revised August 10, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
More informationab Nuclear Extract Kit
Version 1 Last updated 10 November 2017 ab221978 Nuclear Extract Kit For the preparation of nuclear extracts from mammalian and tissue. This product is for research use only and is not intended for diagnostic
More informationNotch Signaling Pathway Notch CSL Reporter HEK293 Cell line Catalog #: 60652
Notch Signaling Pathway Notch CSL Reporter HEK293 Cell line Catalog #: 60652 Background The Notch signaling pathway controls cell fate decisions in vertebrate and invertebrate tissues. Notch signaling
More informationab Mammalian Cell Lysis Buffer 5X
Version 2 Last updated 19 December 2018 ab179835 Mammalian Cell Lysis Buffer 5X For simple and rapid preparation of mammalian cell lysates. View kit datasheet: www.abcam.com/ab179835 (use www.abcam.cn/ab179835
More informationMitochondria/Cytosol Fractionation Kit
ab65320 Mitochondria/Cytosol Fractionation Kit Instructions for Use For the rapid, sensitive and accurate isolation of Mitochondrial and Cytosolic fractions from living cells. This product is for research
More informationMALDI Sepsityper Kit
Instructions for Use MALDI Sepsityper Kit Kit for identification of microorganisms from positive blood cultures using the MALDI Biotyper system CARE products are designed to support our worldwide customers
More information