Effects of muscle spindle afferents on the regulation of respiration in rabbit 3

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1 , Dec. 2000, 52 (6) : Acta Physiologica Sinica 3 3,,, (, ) : 63, (RRN) (Phr1D), (Sch) : (1) Sch, (Ti), (Te), Ti/ Te (RF), ; Te, Ti, Ti/ Te, RF, (2) Sch (37/ 56), (10/ 17), (3) Sch, 1 2 min,, 2 3 min (4), Sch, : ; ; ; : Q424 ; R32213 Effects of muscle spindle afferents on the regulation of respiration in rabbit 3 XING Guo2Gang 3, FAN Xiao2Li, SONG Xin2Ai, LI Qiang ( Department of Physiology, Xi an Medical University, Xi an ) Abstract : Effect of muscle spindle afferent invoked by intraarterial injection of succinylcholine (Sch) on respiration was assessed by changes in unit discharge of respiration related neurons and phrenic nerve of 63 anesthetized, bilaterally vagotomized and artificially ventilated rabbits. The results are as follows. (1) Intra2 arterial injection of Sch prolonged inspiratory duration ( Ti) or decreased expiratory duration ( Te) and aug2 mented respiratory frequency ( RF). (2) 66 % (37/ 56) of the medullary inspiratory neurons were excited and 10/ 17 of the expiratory neurons were inhibited by intraarterial injection of Sch, reaching maximum respectively at 1 2 and 2 3 min after injection, while non2respiration related neurons were not affected. (3) This effect of Sch was suppressed or abolished when muscle spindles were destroyed by intramuscular injection of bupiva2 caine. Key words : muscle spindle afferent ; respiration related neuron ; phrenic nerve discharge ; succinylcholine,,,, ( respiration related neu2 [1,2] ron, RRN) (phrenic nerve discharge,, phr1d), (succinylcholine, Sch),, [3],,,, Received Accepted Corresponding author. Tel : ; E2mail :

2 492 Acta Physiol. Sin. 52 1,, 111 ( kg),, 10 d Fawosky,, 112 (111 g/ kg),,,, 113 (3 mg/ kg) ; ( x s x ), t, P < 0105,,,, 1 2 cm, 211 Sch, Sch, VC SMUP2PC,, Sch 40 ( (RF) (Ti) (Te) %),, 23 (36151 %) ; 17 (26198 %) Ti/ Te, Ti/ Te, 11 (17146 %), 4 (6135 %), 8,, (12170 %), Sch 015 mol/ L ( Sch 2 % ), 5 15 k, , Sch 23 ( %) VC210, ( Ti) ( P < 0105),, SMUP2PC (Te),, Ti/ Te,, ( P < 0101),, ; Sch 1 20 min, Sch (4 mg/ kg,,, 1 ), min, 10 min ( 1) 20 % [4] : 20 % 1. Sch ; 20 % ; Table %, Sch kpa, 10164/ 7198 kpa, Effect of prolonged inspiratory phase induced by intraarterial injection of succinylcholine into rab2 Time (min) Ti (s) Te (s) Ti/ Te, 2 0 (B inj1) % ( 15 U 1 (A inj1) ) 015 ml/ kg, 2 (A inj1) (A inj1) (A inj1) ( ), 10 (A inj1) Sch 15 (A inj1) (A inj1) (R2R 100 n = 23, 3 3 P < 0105, 3 P < 0101 vs control (before injection). 140 ms), CO 2 B inj1 : before injection ; A inj1 : after injection. bits

3 6 : , RF,, Sch 17 ( %) 1 min, 15 min, Te, Ti, Ti/ Te ( 2) 2. Sch Table 2. Effect of attenuated expiratory phase and augmented respiratory frequency induced by in2 traarterial injection of succinylcholine into rabbits Time (min) Ti (s) Te (s) Ti/ Te RF (cyc/ min) 0 (before inj1) (after inj1) (after inj1) (after inj1) (after inj1) (after inj1) (after inj1) (after inj1) n = P < 0105, 3 P < 0101 vs control (before injection). 212 Sch 3. RRN non2rrn Sch Table 3. Distribution of RRNs and non2rrns within the 315 mm 810, mm, mm 78 (RRN) 15 (non2rrn), 3 3, medulla of dorsal and ventral respiratory groups of rabbits (49/ 57 ; 210 mm 210 mm, VRG mm, mm), Total (12/ 21 ; 110 mm IN : inspiratory neuron ; EN : expiratory neuron ; PSN : phase 810 mm, mm, 215 spanning neuron ; DRG: dorsal respiratory group ; VRG: ventral respiratory group. RRN IN EN PSN Total non2rrn DRG Sch Table 4. Effects of intraarterial injection of succinylcholine on spontaneous discharges of respiration re2 RRN lated neurons ( RRNs) and non2respiration related neurons (non2rrns) in rabbit medulla IN Neuron type Neuron numbers Effects of Sch Excitation Depression Biphasic effect No effect Aug1I Dec1I Const1I Late I Total (66107) 11 (19164) 2 (3157) 6 (10172) EN Aug1E Dec1E Const1E Total 17 4 (23153) 10 (58183) 1 (5188) 2 (11176) PSN 5 3 (60100) 1 (20100) 0 ( (20100) non2rrn 15 6 (40100) 5 (33133) 1 (6167) 3 (20100) Aug1I: augmenting IN ; Dec1I: decrementing IN ; Const1I: constant IN ; Late I: late IN ; Aug1E: augmenting EN ; Dec1E: decrementing EN ; Const1E: constant EN. The values in the parentheses represent the percentage ( %).

4 494 Acta Physiol. Sin mm) Sch Sch, (37/ 56), (17/ 19) (15/ 18), (10/ 17),, ; (6/ 6) ( 1 4) 4, 1. Sch Fig1 1. Effects of intraarterial injection of Sch on discharges of respiration related neurons in the medulla and phrenic nerve of rabbit. A C, A 1 C 1. Discharges of an IN and EN respectively. A, A 1. Before Sch. B, B 1. 2 min after Sch. C, C min after Sch. Upper line shows the RRN ; lower line shows the phr1d. Calibration : 10 ms/ Div, 50 V/ Div. D, D 1. Discharge histogram of an IN and EN respectively. : the beginning of Sch injection Sch RRN ( 2) 21 10, 213 Sch, Sch 1 2 min, 3 5 min Sch, 10 min ; 11 6,, 2 3 min,, 5 min, 15 min Sch

5 6 : Sch RRN Fig1 2. Time course of the effect of Sch on respiration related neurons in rabbit medulla. 3 P < 0101 vs control (before injec2 Sch, tion)., [6 ], Sch, ( ) % 1121) % ; ( Ti/ Te) (25186 Sch, 5137) % ( ) %,, ( P < 0101) ( 3) [11 ], 3. Sch Sch Sch Fig1 3. Effect of bupivacaine injection on the effects of dis2 charge of respiration related neurons in the medulla and phrenic nerve induced by Sch injection. A. RRNs. B. Phr1D. 3,, Sch,, (Ti Te Ti/ Te RF ), [5 ], 2, (21194 [7 ], Sch,,, Gautier [8 ] Leit2 ner [3 ] Orani [9 ], Waldrop [10 ] Waldrop, Sch, Sch Waldrop, Sch ( g/ kg), ( mg/ kg) [6 ],,, Waldrop, Sch,, ( ) (8 mg/ kg), Sch N 2,, Waldrop, Sch, ( ), ( ), ( ),, Sch,,

6 496 Acta Physiol. Sin. 52 Sch limb muscle afferents involved in ventilatory effects observed in decerebrate and spinal preparations. Exp Brain Res,, 1993, 92 (3) : Sch, [2 ] Ishida K, Yasuda Y, Miyamura M. Cardiorespiratory re2, sponse at the onset of passive leg movements during sleep in humans. Eur J Appl Physiol, 1993, 66 (6) : , [3 ] Leitner LM, Dejours P. Reflex increase in ventilation induced, by vibrations applied to the triceps surae muscles in the cat. [12 ], Sch Respir Physiol, 1971, 12 (2) : [4 ] Tang B ( ), Fan XL ( ), Wang CY ( ), Sch et al. Role of red nucleus in inhibiting nociceptive responses of rat spindle afferent. Act Physiol Sin ( ), 1999, 51 (4) : (in Chinese with English abstract). [5 ] Nie L ( ), Liu L ( ). The respiratory facilitating Sch effects by electrical and chemical stimulation of central nu2 cleus amygdala. Acta Physiol Sin ( ), 1992, 44 (3), : (in Chinese with English abstract). [6 ] Shi YR ( ), Qin C ( ), Fan XL ( )., Sch, Dose2effect relationship of succinylcholine causing increase of 1 2 min, Sch afferent discharge from muscle spindles in rats. J Xi an Med [13 ] Univ ; ( ), 1996, 17 (1) : (in Chinese with English abstract)., 2 3 min [7 ] Dalsanto G. Kinetics of distribution of radioactive labled, muscle relaxants,3 : Investigations with 15C2Succinyldicholine [14 ] and 14C succinylmocholine during controled conditions.,, Sch Anesthesiology, 1968, 29 (3) : [8 ] Gautier H, Lacaisse A, Dejours P. Ventilatory response to,, muscle spindle stimulation by succinylcholine in cats. Respir Physiol, 1969, 7 (3) : ; [9 ] Orani GP, Decandia M. Group I afferent fibers : effects on, cardiorespiratory system. J Appl Physiol, 1990, 68 (3) : 932 ; 937. [10 ] Waldrop TG, Rybicki KJ, Kaufman MP. Chemical activation of group and muscle afferents has no cardiorespiratory effects. J Appl Physiol, 1984, 56 (5) : , [11 ],.. :, 1993, P27. [12 ] Milburn A. The effect of the local anesthetic bupivacaine on the adult rat muscle spindle. J Physiol ( Lond), 1976, (1) : [13 ] Shi YR ( ), Fan XL ( ), Qin C ( ). Time2effect relationship of succinylcholine causing the in2 crease of afferent discharge from muscle spindle in rat. J Xi an Med Univ ( ), 1996, 17 (2) : (in Chinese with English abstract). [14 ]. 2. ( ) : [1 ] Persegol L, Palisses R, Viala D. Characterization of hind2, 1985,