The HS-Omega-3 Index Methodological Considerations
|
|
- Jean Bell
- 5 years ago
- Views:
Transcription
1
2 The HS-Omega-3 Index Methodological Considerations William S. Harris, PhD The HS-Omega-3 Index is the EPA+DHA content of red blood cells (RBCs) expressed as a percent of total identified RBC fatty acids. It is measured using a proprietary methodology developed over several years of research. OmegaQuant, LLC is a CLIA certified lab (#43D ). Fatty acids (n=24) are quantitatively identified using capillary column gas chromatography with an internal-standard-based, three-point calibration curve approach based loosely on the classic method of Morrison and Smith 1. This method is precise and accurate because it allows for adjustment of day-to-day and instrument-to-instrument variations in FID response factors. This keeps analytical variability very low. Observed Index(%) Predicted Index (%) Figure 1. The correlation between the expected Figure and 1. The observed correlation values between for the the HS-Omega-3 expected and Index observed This values shows that for the across HS-Omega-3 the clinically Index. significant This shows range that across of values, the the clinically test gives significant accurate range results. of values, the test gives accurate results Linearity: The observed HS-Omega-3 Index value matches precisely the expected value when mixtures of RBCs with different amounts of EPA+DHA are analyzed (Figure 1). In addition, the response is linear between 2.5 and 30 ul of RBCs (Figure 2). Precision: Current coefficients of variation (CVs) were determined over 200 runs across 4 instruments. At a low HS-Omega-3 Index (1.7%) the CV is 6.8%, and at a high HS-Omega-3 Index (11.0%) the CV is 2.5%. Sensitivity: The method can reliably detect very low levels of FAs (i.e., 0.1% abundance). GC Area Counts Analytical Linearity RBC volume (ul) Figure Linearity of response of response of the of HS-Omega-3 the HS-Omega-3 Index to Index increasing to increasing amount amount of RBC sample of RBC analyzed. sample analyzed. The test is routinely The test done is routinely with 30 done ul of with packed 30 ul RBCs. of packed R 2 =0.97 RBCs. R=0.97
3 Biological Stability: In a study of 60 placebotreated patients on a stable diet over 10 weeks, the mean HS-Omega-3 Index did not change (4.6±1.% vs. 4.6±1.2%) 2. In another study 3 with 20 healthy volunteers studied 7 times over 6 weeks, the within-subject coefficient of variation (CV) for EPA+DHA in RBCs and whole plasma were 4.1%±1.9% and 15.9%±6.4%, respectively 3 (Figure 3). (The CV for whole blood EPA+DHA was 6.7%±4.0%). Thus, the RBC test had the lowest biological variability which means that a single measurement (not several) is all that is needed to determine the true level. Fasting vs. Fed: The effect of consuming a large meal on the EPA+DHA content of RBCs, whole blood, and plasma was examined in the same 20 subjects as described above3. The EPA+DHA level in RBCs did not differ by fasting-fed status (-1%), but it was slightly lower in whole blood (-5%, p=0.03), and much lower in plasma (-11%, p<0.05) due to the dilution of plasma EPA+DHA with non-omega-3 FAs contained in the test meal. Plasma percent of total FAs RBC percent of total FAs 10% 9% 8% 7% 6% 5% 4% 3% 2% 1% 0% 12% 10% 8% 6% 4% 2% 0% Figure 3. Biologic variability: RBC vs Plasma. EPA+DHA content of both sample types was measured weekly in 20 volunteers for 6 weeks. Determination of the HS-Omega-3 Index from a Dried Blood Spot (DBS): The DBS method for determining the HS-Omega-3 Index was validated in a study including 106 subjects from whom DBS cards and blood tubes were collected. The latter were immediately processed for RBCs which were frozen at -80 C; the former were express shipped to various cities around the country, and from there, returned by regular US mail back to OmegaQuant for routine analysis. The correlation between the EPA+DHA content of the two samples was very high (r=0.96; p<0.0001), and the 95% confidence interval for the DBS-estimated value was ± 1% (Figure 4). RBC EPA+DHA DBS EPA+DHA Figure 4. Correlation between the EPA+DHA content of dried blood spots (DBS) and the (RBCbased) HS-Omega-3 Index. DBS samples were collected from 106 subjects, shipped via standard US mail, and compared to RBC samples frozen immediately after collection. R=0.96, p<
4 Preventing Loss of EPA and DHA on Dried Blood Spots with OxyStop : The long-chain omega-3 fatty acids that comprise the HS-Omega-3 Index (EPA and DHA) are highly polyunsaturated and therefore very susceptible to oxidative degradation. At OmegaQuant, we use our proprietary antioxidant treatment called OxyStop to retard this natural process in samples collected as dried blood spots on filter paper (QuantCards ). In the experiment illustrated in Figure 5, one blood sample was applied to 3 different types of card/antioxidant systems: QuantCards with OxyStop, Fluka cards without BHT and Fluka cards with BHT. (Fluka cards and BTH are available from Sigma- Aldrich). Cards were prepared in an identical manner and stored in plastic bags in the dark at room temperature for up to 2 weeks. On the indicated day, one of each type of card was removed, 3 punches of dried blood were taken from each, and then all were analyzed for the HS-Omega-3 Index. The average of each triplicate analysis is plotted. There was no loss of EPA and DHA in the OxyStop-treated QuantCard whereas there was loss in the Fluka cards, less with BHT than without. HS-Omega-3 Index 9% 8% 7% 6% 5% 4% 3% 2% 1% OxyStop Fluka with BHT Fluka without BHT 0% DayDays Figure 5. Comparison of QuantCards with OxyStop v Figure Fluka 5. cards Comparison with and of without QuantCards BHT. The with HS-Omega-3 OxyStop vs Index Fluka was cards measured with and in without triplicate BHT. at The the HS-Omega-3 Index was measured in triplicate on the indi- indicated day cated day. Recovery of Fatty Acids from Spiked Samples: Experiments were conducted to determine the a- ccuracy of both RBC and plasma fatty acid analytical methods. Accuracy was defined as the percent recovery of 6 major fatty acids, all esterified in phosphatidylcholine (their primary chemical form in plasma and RBCs), added to native RBC and plasma samples. The analyses were performed in triplicate using three levels of added fatty acids analyzed with the internal-standard, 3-point calibration curve method. Table 1 shows the fatty a- cids assessed and the percent recoveries. Clearly, the method quantitatively determines the fatty acid content of both sample types. TABLE 1: Fatty Acid Recovery % Recovery Fatty Acid RBC Plasma C16:0 98% 98% C18:0 98% 98% C18:1n9 101% 102% C18:2n6 101% 98% C20:4n6 100% 94% C22:6n3 104% 98%
5 EDTA Blood Samples Analyzed Wet Vs Dry on QuantCards: Some researches prefer to send a spot of blood rather than a tube of blood to OmegaQuant. In order to document comparability of these two sample types, EDTA (lavender top) tubes were drawn from 20 subjects. The tubes were opened, a drop of blood was placed on a QuantCard and allowed to dry. The card and the liquid blood sample were then analyzed. The mean ±SD HS-Omega-3 Index was 5.08±1.9% for the EDTA-blood and 5.05±1.9% for blood card. The correlation was 0.97 (Figure 6). Comparison with Other Laboratories: International, cross validation studies were conducted with two other laboratories that are using the HS-Omega-3 Index technology [OmegaQuant Asia (Seoul, Korea) and OmegaMetrix (Munich, Germany)]. The HS-Omega-3 Index measured in these labs varied by less than 0.1 percentage points from the value obtained at OmegaQuant. This confirms that the test is transferrable to other labs and that accuracy and precision can be achieved when identical methodologies are employed. Different methodologies, however, can generate different results. To illustrate, a comparison was made using 366 RBC samples. In this study, the samples were analyzed at OmegaQuant using the HS-Omega-3 technology, and in Lab X (in Scandinavia) using their own methodology. Values are significantly higher for nearly every fatty acid in the OmegaQuant analysis (see Table 2 showing selected fatty acids). The reason for this difference has more to do with the denominator than the numerator. In other words, since fatty acid levels are expressed as a percent of total, deciding which fatty acids are included in the denominator (the total ) can influence the results. At OmegaQuant we focus our analysis on the fatty acids esterified in a particular, physiologically-active subset of the membrane glycerophospholipids. Lab X uses another method which measures these plus other types of fatty acids (italics in the Table 2) predominantly carried in sphingolipids. The latter fatty acids add about 11% to the denominator. In addition, Lab X includes the area under ALL peaks on the chromatogram (whether positively identified as fatty acids or not, unknown ), whereas we include only a suite of the most important, clearly identified fatty acids in our calculations. Fingerstick (%) Fatty Acid EDTA blood (%) Figure Comparison of the HS-Omega-3 Index Index measured on dried blood spots spots using using blood blood collected collected either from either a fingerstick from a or fingerstick from a EDTA or tube from (n=20). a EDTA tube (n=20) TABLE 2: Inter-lab Comparison Lab X % of fatty acids OMQ C16: C16:1n C18: C18: 1 trans C18: C18:2n C20:4n C20:5n C22: C24: C24:1n C22:4n C22:5n C22-6n Omega-3 Index Unknown
6 So the fact that Lab X includes more/different fatty acid species in the denominator reduces the apparent levels of the Omega-3 Index as well as all other fatty acids. Not only are there are no standardized conventions defining which fatty acids should be included in the denominator, there is no consensus regarding how the fatty acids are chemically liberated from the RBC samples, nor for how baselines are defined for each type of peak. Therefore, differences in methods can result in very different fatty acid compositions. As another example, the same blood sample was sent to 4 commercial US labs for analysis of the omega-3 index (Figure 7). Compared to the OmegaQuant value (10.4%), the other labs returned values varying from 3.81% to 13.74%. This underscores the wide variability in results that can be obtained commercially. Omega-3 Index (%) OMQ LABG LABM LABB LABN Figure 7. The same blood sample was sent to 4 commercial labs in the US for measurement Figure of omega-3 7. The index. same The results blood confirm sample lab to lab was s to differences. 4 commercial labs in the US for measurement of the omega-3 index. Th results confirm that lab to lab differenc OmegaQuant The Gold Standard: We consider the OmegaQuant results to be the gold standard because our unique HS-Omega-3 Index technology has been and continues to be used in several major epidemiologic 4-13 and intervention studies. These studies are, for the first time, linking specific fatty acid values with risk for important clinical outcomes (death, heart attacks, depression, dementia, etc). Thus, investigators and clinicians wanting to take an evidence-based approach to monitoring and titrating their patients omega-3 status must use the test that was originally used to derive the evidence. For example, if an HS-Omega-3 Index of 8% or greater is considered cardioprotective based on several published studies using OmegaQuant, then the healthcare provider who used Lab M or Lab B (Figure 7) will mistakenly conclude that his/her patient needs to increase their omega-3 intake. In like manner, a sample sent to Lab G, which overestimated the Omega-3 Index by 3.4%, could be falsely reported as being in the target zone when an increased omega-3 intake should have been prescribed. With the HS-Omega-3 Index clinicians can be confident that they are using the most researched omega-3 status test available.
7 Reference List (1) Morrison WR, Smith LM. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J Lipid Res 1964;5: (2) Carney RM, Freedland KE, Rubin EH et al. Omega-3 augmentation of sertraline in treatment of depression in patients with coronary heart disease: a randomized controlled trial. JAMA ;302: (3) Harris WS, Thomas RM. Biological variability of blood omega-3 biomarkers. Clin Biochem ;43: (4) Block RC, Harris WS, Reid KJ et al. EPA and DHA in Blood Cell Membranes from Acute Coro nary Syndrome Patients and Controls. Atherosclerosis 2007;197: (5) Block RC, Harris WS, Reid KJ et al. Omega-6 and trans fatty acids in blood cell membranes: a risk factor for acute coronary syndromes? Am Heart J 2008;156: (6) Cohen BE, Garg SK, Ali S et al. Red blood cell docosahexaenoic acid and eicosapentaenoicacid concentrations are positively associated with socioeconomic status in patients with established coronary artery disease: data from the heart and soul study. J Nutr 2008;138: (7) Farzaneh-Far R, Lin J, Epel ES et al. Association of marine omega-3 fatty acid levels with telo meric aging in patients with coronary heart disease. JAMA 2010;303: (8) Farzaneh-Far R, Harris WS, Garg S et al. Inverse association of erythrocyte n-3 fatty acid levels with inflammatory biomarkers in patients with stable coronary artery disease: The Heart and Soul Study. Atherosclerosis 2009;205: (9) Harris WS, Assaad B, Poston WC. Tissue omega-6/omega-3 fatty acid ratio and risk for coro nary artery disease. Am J Cardiol 2006;98:19i-26i. (10) Moyers B, Farzaneh-Far R, Harris WS et al. Relation of Whole Blood n-3 Fatty Acid Levels to Exercise Parameters in Patients With Stable Coronary Artery Disease (from the Heart and Soul Study). Am J Cardiol :1/49-54 (11) Shearer GC, Pottala JV, Spertus JA et al. Red blood cell fatty acid patterns and acute coronary syndrome. PLoS ONE 2009;4:e5444. (12) Baghai TC, Varallo-Bedarida G, Born C et al. Major depressive disorder is associated with car diovascular risk factors and low Omega-3 index. J Clin Psychiatry 2010 (epub). (13) Harris WS, Sands SA, Windsor SL et al. Omega-3 Fatty Acids in Cardiac Biopsies from Heart Transplant Patients: Correlation with Erythrocytes and Response to Supplementation. Circula tion 2004;110: (14) Harris WS, Gonzales M, Laney N et al. Effects of omega-3 fatty acids on heart rate in cardiac transplant recipients. Am J Cardiol 2006;98: (15) Harris WS, Pottala JV, Sands SA et al. Comparison of the effects of fish and fish-oil capsules on the n 3 fatty acid content of blood cells and plasma phospholipids. Am J Clin Nutr 2007;86: (16) Harris WS, Lemke SL, Hansen SN et al. Stearidonic acid-enriched soybean oil increased the omega-3 index, an emerging cardiovascular risk marker. Lipids 2008;43: (17) Larson MK, Ashmore JH, Harris KA et al. Effects of omega-3 acid ethyl esters and aspirin, alone and in combination, on platelet function in healthy subjects. Thromb Haemost 2008;100: OmegaQuant, OxyStop and HS-Omega-3 Index are registered marks of Harris Scientific, Inc All rights reserved.
8
Automated Analysis and Quantitation of Fish Oil Supplements using the MIDI Sherlock Marine Oil Analysis Package
125 Sandy Drive, Newark, Delaware 19713 USA tel: 302-737-4297 fax: 302-737-7781 www.midi-inc.com Automated Analysis and Quantitation of Fish Oil Supplements using the MIDI Sherlock Marine Oil Analysis
More informationOptimizing The Omega-3 Index with Krill Oil By Lena Burri, PhD
Optimizing The Omega-3 Index with Krill Oil By Lena Burri, PhD THE OMEGA-3 INDEX is a diagnostic tool that is quickly becoming more popular with medical professionals and consumers. Beyond assessing cardiovascular
More informationShedding new light on heart health: Examining recent data on fatty acids
Shedding new light on heart health: Examining recent data on fatty acids London, 18 November 2015 Prof. Dr. C. von Schacky, FESC Preventive Cardiology Medizinische Klinik und Poliklinik I Ludwig Maximilians-Universität
More informationRapid Analysis of 37 FAMEs with the Agilent 8860 Gas Chromatograph
Application Note Food Rapid Analysis of 37 FAMEs with the Agilent 88 Gas Chromatograph Author Youjuan Zhang Agilent Technologies (Shanghai) Co. Ltd., Shanghai 131 P. R. China Abstract An Agilent 88 GC
More informationPredictors of Omega-3 Index in Patients With Acute Myocardial Infarction
Original Article Predictors of Omega-3 in AMI Predictors of Omega-3 Index in Patients With Acute Myocardial Infarction Adam C. Salisbury, MD, MSc; Amit P. Amin, MD; William S. Harris, PhD; Paul S. Chan,
More informationDetermination of red blood cell fatty acid profiles in clinical research
Application Note Clinical Research Determination of red blood cell fatty acid profiles in clinical research Chemical ionization gas chromatography tandem mass spectrometry Authors Yvonne Schober 1, Hans
More informationAnalysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column
Application Note Food Analysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column Authors Ingrid Van Der Meer, Yun Zou, and Gustavo Serrano Agilent
More informationFuture directions for nutritional and therapeutic research in omega-3 3 lipids
Future directions for nutritional and therapeutic research in omega-3 3 lipids Philip Calder Professor of Nutritional Immunology University of Southampton Aim To review dietary sources and intakes of long
More informationThere is more scientific evidence behind the cardiovascular benefits of fish oil than nearly any other nutritional supplement
n Provides optimal support for heart health n Reduces triglyceride levels n Reduces cardiovascular risk factors 7, 8 n Complements statin drug therapy n Optimizes circulation and blood vessel function
More informationCorrelation of omega-3 levels in serum phospholipid from 2053 human blood samples with key fatty acid ratios
Nutrition Journal This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Correlation of omega-3 levels
More informationAOAC Research Institute Performance Tested Methods SM Program. Measurement of Free Fatty Acids Using FASafe Standard Test Kit. Certificate Report
Performance Tested Methods SM Program Measurement of Free Fatty Acids Using FASafe Standard Test Kit Certificate Number 030405 June 23, 2003 SāfTest, Inc.,. 2414 West 12 th Street, Suite 4 Tempe, Arizona
More information17 MAY SEOUL. IADSA Annual General Meeting Codex Alimentarius / Omega 3
17 MAY SEOUL IADSA Annual General Meeting Codex Alimentarius / Omega 3 Codex Alimentarius CCNSFDU NRV/NCD EPA DHA NRV NCD BASIS FOR NRVs-NCD Need for: What are NRVs-NCD? NRVs-NCD stands for Nutrient Reference
More informationCHAPTER 1 INTRODUCTION
1 CHAPTER 1 INTRODUCTION 1.1 Background and Rationale Thailand has become the world's largest exporter of canned tuna and largest importer of fresh and frozen tuna. Canned tuna exports accounted for 77%
More informationFacts on Fats. Ronald P. Mensink
Facts on Fats Ronald P. Mensink Department of Human Biology NUTRIM, School for Nutrition, Toxicology and Metabolism Maastricht University Maastricht The Netherlands Outline of the Presentation Saturated
More informationCarnitine / Acylcarnitines Dried Blood Spots LC-MS/MS Analysis Kit User Manual
Page 1 / 14 Carnitine / Acylcarnitines Dried Blood Spots LC-MS/MS Analysis Kit User Manual ZV-3051-0200-15 200 Page 2 / 14 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST
More informationTransplantation. Omega-3 Fatty Acids in Cardiac Biopsies From Heart Transplantation Patients
Transplantation Omega-3 Fatty Acids in Cardiac Biopsies From Heart Transplantation Patients Correlation With Erythrocytes and Response to Supplementation William S. Harris, PhD; Scott A. Sands, PhD; Sheryl
More informationAutomated Sample Preparation for FAME Analysis in Edible Oils Using an Agilent 7696A Sample Prep WorkBench
Automated Sample Preparation for FAME Analysis in Edible Oils Using an Agilent 7696A Sample Prep WorkBench Application Note Authors Rima Juskelis and Jack Cappozzo Institute for Food Safety and Health
More informationDifferentiating omega-3 fatty acids from SPMs (specialized pro-resolving lipid mediators)
NIHR Southampton Biomedical Research Centre in nutrition Differentiating omega-3 fatty acids from SPMs (specialized pro-resolving lipid mediators) Philip Calder Professor of Nutritional Immunology University
More informationHigh trans fatty acid intake reduces the relative ω-3 fatty acid content in the erythrocyte membrane.
Biomedical Research 2017; 28 (17): 7693-7697 ISSN 0970-938X www.biomedres.info High trans fatty acid intake reduces the relative ω-3 fatty acid content in the erythrocyte membrane. Qiang Peng 1, Yin Li
More informationFish oil and cardiovascular science supporting global regulatory efforts
Fish oil and cardiovascular science supporting global regulatory efforts Dr Harry Rice Your essential event for networking and information Fish Oil and Cardiovascular Science Supporting Global Regulatory
More informationNutracode KrillCode A premium omega 3 solution.
Nutracode KrillCode A premium omega 3 solution Krill Small Creature Big Advantage Antarctic Krill (Euphausia Superba) Antarctic Krill are swimming crustaceans that live in the Antarctic waters of the Southern
More informationVOLUNTARY MONOGRAPH. Council for Responsible Nutrition March 2006
VOLUNTARY MONOGRAPH Council for Responsible Nutrition March 2006 Omega-3 DHA Omega-3 EPA Omega-3 DHA & EPA DEFINITION Omega-3 fatty acids, EPA and DHA, consist of the all cis forms of 5, 8, 11, 14, 17-eicosapentaenoic
More informationFatty Acid Methylation Kits
Methyl esterification kit for fatty acids analysis Fatty Acid Methylation Kits Below are two methods for efficiently preparing fatty acid samples for GC analysis. Neither method requires high temperatures,
More informationThe effect of plant sterols and different low doses of omega-3 fatty acids from fish oil on lipoprotein subclasses
The effect of plant sterols and different low doses of omega-3 fatty acids from fish oil on lipoprotein subclasses 18 June 2015 Doris Jacobs Unilever R&D Vlaardingen Background Elevated low-density lipoprotein-cholesterol
More informationGender-specific differences in essential fatty acid metabolism
Note: for non-commercial purposes only Gender-specific differences in essential fatty acid metabolism Tamás Decsi and Kathy Kennedy Department of Paediatrics, University of Pécs, Pécs, Hungary Nutrition
More informationJes S. Lindholt. The role of fish oil in the natural history of abdominal aortic aneurysms. J Am Heart Assoc Jan 26;7(3).
The role of fish oil in the natural history of abdominal aortic aneurysms J Am Heart Assoc. 2018 Jan 26;7(3). Jes S. Lindholt Professor in Vascular Surgery, DMSci, Ph.D. Odense University Hospital, Denmark.
More informationApplication Note. Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice. Author. Abstract.
Agilent Application Solution Analysis of ascorbic acid, citric acid and benzoic acid in orange juice Application Note Author Food Syed Salman Lateef Agilent Technologies, Inc. Bangalore, India 8 6 4 2
More informationLipoprotein Particle Profile
Lipoprotein Particle Profile 50% of people at risk for HEART DISEASE are not identified by routine testing. Why is LPP Testing The Most Comprehensive Risk Assessment? u Provides much more accurate cardiovascular
More informationLipid Analysis. Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th Introduction to lipid structures
Lipid Analysis Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th 2005 Introduction to lipid structures Fatty acids Acylglycerols Glycerophospholipids Sterols Strategies involved in lipid
More informationretardation in infants. A wide variety of analytical methods for the analysis of
IN THE NAME OF GOD Diagnosis and measurment of Phenylalanine in plasma and dried bilood spot (DBS) by a simple and sensitive HPLC method and compaire with Recipe Reference Health Laboratory Research Center,
More informationThe role of omega-3 fatty acids as a treatment for bipolar disorder
The role of omega-3 fatty acids as a treatment for bipolar disorder Research into omega-3 fatty acids as a possible treatment for mood disorders is a new and interesting area of research Although research
More informationAutomated Sample Preparation for Profiling Fatty Acids in Blood and Plasma using the Agilent 7693 ALS
Automated Sample Preparation for Profiling Fatty Acids in Blood and Plasma using the Agilent 7693 ALS Application Note Clinical Research Authors Frank David and Bart Tienpont, Research Institute for Chromatography,
More informationOmega-3 Fatty Acids. Alison L. Bailey MD, FACC Erlanger Heart and Lung Institute/University of Tennessee COM
Omega-3 Fatty Acids Alison L. Bailey MD, FACC Erlanger Heart and Lung Institute/University of Tennessee COM Chattanooga @a_l_bailey Disclosures None Objectives Describe omega-3 fatty acids Review the historic
More informationUNDERGRADUATE RESEARCH LONG TERM GRANT APPLICATION Budget Worksheet
UNDERGRADUATE RESEARCH LONG TERM GRANT APPLICATION Budget Worksheet BUDGET ITEM Department or College Funds Outside Agency Funds Personal Funds Undergrad. Research Funds GRAND TOTAL Materials $3,238.00
More informationFast quantitative Forensic Analysis of THC and its Metabolites in Biological Samples using Captiva EMR- Lipid and LC/MSMS
Fast quantitative Forensic Analysis of THC and its Metabolites in Biological Samples using Captiva EMR- Lipid and LC/MSMS Christophe Deckers, M.Sc. Sample prep Application Scientist Types of ``Interferences``
More informationSCIEX Vitamin D 200M Assay for the Topaz System
The First FDA-Cleared LC-MS/MS Assay for Vitamin D SCIEX Vitamin D 200M Assay for the Topaz System The first FDA-cleared LC-MS/MS assay for Vitamin D Vitamin D is an important building block for human
More information14 DAGGERS OF ARTERIAL DISEASE
14 DAGGERS OF ARTERIAL DISEASE This image depicts daggers aimed at a healthy heart. Any one of these daggers would kill if thrust deep into the heart. In the real world, however, aging humans suffer small
More informationImproving the Analysis of 37 Fatty Acid Methyl Esters
Application Note Food Testing Improving the Analysis of 37 Fatty Acid Methyl Esters Using Three Types of Capillary GC columns Authors Yun Zou Agilent Technologies (Shanghai) Co.Ltd, Shanghai 200131 P.R.China
More informationResearch shows that the most reliable source of omega-3s is a high-quality fish oil supplement
n Helps the body naturally address occasional eye irritation n Promotes healthy eye moisture for occasional dry eyes n Promotes normal eye function as we age n Contains the fatty acid DHA, found in greatest
More informationLOVAZA (omega-3-acid ethyl esters) oral capsule VASCEPA (icosapent ethyl) oral capsule
VASCEPA (icosapent ethyl) oral capsule Coverage for services, procedures, medical devices and drugs are dependent upon benefit eligibility as outlined in the member's specific benefit plan. This Pharmacy
More informationSupplementary Online Content
Supplementary Online Content Irish AB, Viecelli AK, Hawley CM, et al; for the Omega-3 Fatty Acids (Fish Oils) and Aspirin in Vascular Access Outcomes in Renal Disease (FAVOURED) Study Collaborative Group.
More informationThe UCD community has made this article openly available. Please share how this access benefits you. Your story matters!
Provided by the author(s) and University College Dublin Library in accordance with publisher policies., Please cite the published version when available. Title Omega-3s: - how much is in your salmon? Authors(s)
More informationFeeding Feedlot Steers Fish Oil Differentially Enhances the Fatty Acid Composition of Muscle Tissue 1
Feeding Feedlot Steers Fish Oil Differentially Enhances the Fatty Acid Composition of Muscle Tissue 1 T.J. Wistuba 2,3, E.B. Kegley 2, J.K. Apple 2, and D.C. Rule 4 Story in Brief Inclusion of fish oil,
More informationAAOCS 10 th Biennial Conference Barossa Valley, September Presenter: Petter-Arnt Hals MSc PhD Co-authors: Nils Hoem, Xiaoli Wang, Yong-Fu Xiao
Effects of a purified, omega-3 rich krill oil phospholipid on cardiovascular disease risk factors and fatty acid composition of erythrocyte membranes in non-human primates AAOCS 10 th Biennial Conference
More informationLC-MS/MS Method for the Determination of Tenofovir from Plasma
LC-MS/MS Method for the Determination of Tenofovir from Plasma Kimberly Phipps, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 687 Key Words SPE, SOLA CX, Hypersil GOLD, tenofovir Abstract
More informationA MODIFICATION OF GAS CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF FATTY ACID COMPOSITION OF MILK FAT
1014 Bulgarian Journal of Agricultural Science, 22 (No 6) 2016, 1014 1020 Agricultural Academy A MODIFICATION OF GAS CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF FATTY ACID COMPOSITION OF MILK FAT G.
More informationTechnical Information Guide
Technical Information Guide This Guide provides information relating to the selection, utilization, and interpretation of the Quantose IR test. Information provided is based on peer-reviewed publications
More informationImproving the Analysis of Fatty Acid Methyl Esters Using Automated Sample Preparation Techniques
Improving the Analysis of Fatty Acid Methyl Esters Using Automated Sample Preparation Techniques Rebecca Veeneman Agilent Technologies Pittcon 2011 March 16, 2011 1370-5 Outline Introduction Fatty acids
More informationSuper Omega-3 EPA/DHA with Sesame Lignans & Olive Extract
Better Nutrition 2016 Best of Supplements Award Winner http://www.lifeextension.com Super Omega-3 EPA/DHA with Sesame Lignans & Olive Extract Get enough omega-3s 120 softgels Item Catalog Number: 01982
More informationA RAPID AND SENSITIVE ANALYSIS METHOD OF SUDAN RED I, II, III & IV IN TOMATO SAUCE USING ULTRA PERFORMANCE LC MS/MS
A RAPID AD SESITIVE AALYSIS METD OF SUDA RED I, II, III & IV I TOMATO SAUCE USIG ULTRA PERFORMACE LC MS/MS Choon Keow G, aomi TAAKA, Michelle KIM, Swee Lee YAP Waters Asia, Regional Technology Center,
More informationComparative Study of Fat (Total Cholestrol and Fatty acids) Profile in Farm cultivated and river water fishes communities of Labeo rohita
International Journal of Scientific and Research Publications, Volume 7, Issue 7, July 2017 763 Comparative Study of Fat (Total Cholestrol and Fatty acids) Profile in Farm cultivated and river water fishes
More informationCetoleic acid makes pelagic fish more healthy
Cetoleic acid makes pelagic fish more healthy WORKSHOP IN FISHMEAL AND FISH OIL, NOVEMBER 2018 Bente Ruyter Nofima Omega-3 fatty acids and health Eye Brain Cell membrane The marine omega-3 fatty acids
More informationInterested in conducting your own webinar?
Interested in conducting your own webinar? Email webinars@bnpmedia.com An Automated System for the analysis of fatty acid methyl esters (FAME) in edible oils Institute for Food Safety and Health Illinois
More informationDr Jeff Leighton, Chief Science Officer and Vanessa Whiting, Senior Nutritionist
Dr Jeff Leighton, Chief Science Officer and Vanessa Whiting, Senior Nutritionist # of Health Benefits CV..2-3 grams TGs.4 grams Inflammation..> 4 grams CNS? Women s Health.? G.I. (Leaky Gut)..? DOSE Variables:
More informationThe use of mass spectrometry in lipidomics. Outlines
The use of mass spectrometry in lipidomics Jeevan Prasain jprasain@uab.edu 6-2612 utlines Brief introduction to lipidomics Analytical methodology: MS/MS structure elucidation of phospholipids Phospholipid
More informationFISH. College of Applied Medical Sciences Department of Community Health Sciences Clinical Nutrition Functional Foods CHS 457 Level 9
College of Applied Medical Sciences Department of Community Health Sciences Clinical Nutrition Functional Foods CHS 457 Level 9 FISH Presented By: Nada Abdullah Al-Akeil Supervised By: Mrs.Madawi Al-Dhwayan
More informationusing the Agilent 7696A Sample Prep
Automated Clean-up for Mineral Oil (Hydrocarbon Oil Index) Analysis using the Agilent 7696A Sample Prep WorkBench Application Note Automated Sample Preparation Authors Frank David, Karine Jacq, and Bart
More informationColumn Selection for the Analysis of Fatty Acid Methyl Esters Application
Column Selection for the Analysis of Fatty Acid Methyl Esters Application Food Analysis Authors Frank David Research Institute for Chromatography President Kennedy Park B- Kortrijk, Belgium Pat Sandra
More informationTHE HEALTH AND RETIREMENT STUDY: INVESTIGATION OF DRIED BLOOD SPOT HBA1C MEASUREMENT ERRORS USING TIPTEMP STAMPS
: INVESTIGATION OF DRIED BLOOD SPOT HBA1C MEASUREMENT ERRORS USING TIPTEMP STAMPS April 29, 2015 PAA Biomarker Network Meeting Jessica Faul, Ph.D., M.P.H. Health and Retirement Study Survey Research Center
More informationOptimize Your Omega-3 Status Personalized Blood Test Reveals a Novel Cardiac Risk Factor
http://www.lef.org/ Life Extension Magazine May 2010 Optimize Your Omega-3 Status Personalized Blood Test Reveals a Novel Cardiac Risk Factor By Julius Goepp, MD Suppose you could assess with precision
More informationSERVICE SCHEDULE, FEES & GUIDELINES FOR SAMPLE SHIPMENT
SERVICE SCHEDULE, FEES & GUIDELINES FOR SAMPLE SHIPMENT ACYLCARNITINE PROFILE: PLASMA/SERUM, PKU CARD, WHOLE BLOOD Amount of sample: Plasma (heparinized is preferred, EDTA acceptable) or serum: 0.5 ml
More informationEssential Fatty Acids Essential for Good Health SIE
Page 1 of 6 Essential Fatty Acids Essential for Good Health SIE By Yousry Naguib, PhD Essential fatty acids (EFAs) must be obtained through the diet and cannot be synthesized by the human body. EFAs are
More informationDETERMINATION OF FATTY ACIDS IN EDIBLE OILS BY CAPILARY GC
DETERMINATION OF FATTY ACIDS IN EDIBLE OILS BY CAPILARY GC Vesna Kostik 1 University Goce Delcev Stip Faculty of Medicine Department of Pharmacy 1 WHY FATTY ACID (FA) ANALYSIS IN EDIBLE OILS The content
More informationWhat Makes a Satisfactory Newborn Screen Sample?
What Makes a Satisfactory Newborn Screen Sample? Feb 21, 2013 Christine McRoberts Newborn Screening Ontario One of the challenges the Newborn screening Ontario program (NSO) faces every day is the receipt
More informationRe: Food-frequency questionnaire for assessing long-chain ω-3 fatty-acid intake
University of Wollongong Research Online Faculty of Science, Medicine and Health - Papers Faculty of Science, Medicine and Health 2013 Re: Food-frequency questionnaire for assessing long-chain ω-3 fatty-acid
More informationComparison of the effects of fish and fish-oil capsules on the n 3 fatty acid content of blood cells and plasma phospholipids 1 3
Comparison of the effects of fish and fish-oil capsules on the n 3 fatty acid content of blood cells and plasma phospholipids 1 3 William S Harris, James V Pottala, Scott A Sands, and Philip G Jones ABSTRACT
More informationRapid Separation of Fatty Acid Methyl Esters
Application Note Food Testing & Agriculture Rapid Separation of Fatty Acid Methyl Esters Using DB-FastFAME Intuvo GC columns Author Yun Zou Agilent Technologies (Shanghai) Co. Ltd. Shanghai 200131 P. R.
More informationComparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D
Comparison of Full Scan MS2 and MS3 Linear Ion Trap Approaches for Quantitation of Vitamin D Julie A. Horner 1, Marta Kozak 1, Subodh Nimkar 1, and August A. Specht 1 1 Thermo Fisher Scientific, San Jose,
More informationJanussan Gunasingham. A thesis. presented to the University of Waterloo. in fulfillment of the. thesis requirement for the degree of
Dried Blood Spot Fatty Acid Quantitation: Developing Methods for Determining Unknown Volumes, Identifying Contaminants and Assessing Fatty Acid Stability During Storage by Janussan Gunasingham A thesis
More informationFish Oils and Stroke/Blood Coagulation
Fish Oils and Stroke/Blood Coagulation Summaries of the latest research concerning fish oils and stroke and blood coagulation Review supports benefits of omega-3 fatty acids for prevention of heart disease
More informationName of Policy: Measurement of Long-Chain Omega-3 Fatty Acids in Red Blood Cell Membranes as a Cardiac Risk Factor
Name of Policy: Measurement of Long-Chain Omega-3 Fatty Acids in Red Blood Cell Membranes as a Cardiac Risk Factor Policy #: 239 Latest Review Date: July 2010 Category: Laboratory Policy Grade: Active
More informationObjectives 4/4/2013. Healing with Fats and Fatty Acids-- an Integrative approach. Inflammation Nation. A silent attack on the modern human race
Healing with Fats and Fatty Acids-- an Integrative approach Tracy S. Hunter, RPh, MS, PhD Professor Wingate University School of Pharmacy Charlotte Metro-area, NC 1 Objectives Explain the relationship
More informationAnalysis of Cholesterol-Lowering Drugs (Statins) Using Dried Matrix Spot Technology
Analysis of Cholesterol-Lowering Drugs (Statins) Using Dried Matrix Spot Technology Application Note Small Molecule Pharmaceuticals and Generics Authors Ritu Arora, William udson, Ben Yong, and Paul Boguszewski
More informationHIV and Co-morbidities November 18, 2013, 3:10 pm Abstract Number 141
Low omega-3 index in erythrocytes is a risk factor for progression of atherosclerosis in people living with HIV Bianca M Arendt, M Smieja, IE Salit, DWL Ma, F Smaill, D Elston, E Lonn, Johane P Allard
More informationAN IMPORTANT GUIDE TO HEALTH. Very high triglycerides may. put you at risk. Fortunately, there is. a proven way. to lower them. Ask your doctor.
AN IMPORTANT GUIDE TO HEALTH Very high triglycerides may put you at risk. Fortunately, there is a proven way to lower them. Ask your doctor. What is LOVAZA? LOVAZA is the first and only prescription omega-3
More informationOmega 3 s and Cardiovascular Disease: High vs. Low Dose? Terry A. Jacobson M.D., F.N.L.A. Emory University Atlanta, GA
Omega 3 s and Cardiovascular Disease: High vs. Low Dose? Terry A. Jacobson M.D., F.N.L.A. Emory University Atlanta, GA Structure of Omega-3 and Omega-6 Fatty Acids Omega-6 fatty acids CH 3 Omega-3 fatty
More informationALTERATIONS IN NEURAL FATTY ACID METABOLISM CAUSED BY VITAMIN E DEFICIENCY
ALTERATIONS IN NEURAL FATTY ACID METABOLISM CAUSED BY VITAMIN E DEFICIENCY Bonnie Buckingham Faculty Mentor: Dr. Maret Traber Linus Pauling Institute HHMI Summer 2011 Outline Significance Background Hypothesis
More informationA Robustness Study for the Agilent 6470 LC-MS/MS Mass Spectrometer
A Robustness Study for the Agilent 7 LC-MS/MS Mass Spectrometer Application Note Clinical Research Authors Linda Côté, Siji Joseph, Sreelakshmy Menon, and Kevin McCann Agilent Technologies, Inc. Abstract
More informationDietary neutral lipid level and source affect food intake, nutrient absorption, gut structure, enzymatic activity and growth in marine fish larvae
Dietary neutral lipid level and source affect food intake, nutrient absorption, gut structure, enzymatic activity and growth in marine fish larvae S. Morais 1, L.E.C. Conceição 1, W. Koven 2, I. Rønnestad
More informationTEST REPORT Balance 4Life
TEST REPORT Balance 4Life Fatty Acids Profile and Dietary Advice Your Summary Result Welcome to ZinzinoTest Report The test scores tells you if your diet is pro-, semi- or anti-inflammatory, so you can
More informationLC-MS/MS quantitative analysis of Polyunsaturated Omega 3, 6,7 and 9 Fatty Acids in Serum for
POSTER NOTE 64921 LC-MS/MS quantitative analysis of Polyunsaturated Omega 3, 6,7 and 9 Fatty Acids in Serum for Research Use LC-MS/MS quantitative analysis of Poly Rory M Doyle*, Thermo Scientific, Inc,
More informationFATTY ACIDS IN PLASMA BY GC/MS - Code GC75010
FATTY ACIDS IN PLASMA BY GC/MS - Code GC75010 BIOCHEMISTRY The term fatty acids (abbreviation FA, English Fatty Acids) are indicated aliphatic monocarboxylic acids. They are, with few exceptions, long
More informationINTEGRATIVE MEDICINE BLOOD - EDTA Result Range Units
D Collected : 00-00-0000 INTEGRATIVE MEDICINE BLOOD - EDTA Result Range Units RED CELL FATTY ACID PROFILE Red Cell Fatty Acid Summary Saturated Fats, Total 36.99 19.30-39.40 Monounsaturated Fats, Total
More informationRecent Advances in the Analysis of Ethanol in Saliva: Evaluation of the QED Device
Recent Advances in the Analysis of Ethanol in Saliva: Evaluation of the QED Device A.W. Jones and k A. Jünsson Departments of Alcohol Toxicology and Internal Medicine, University Hospital, 581 85 Linköping,
More informationA New Method for the Early Detection of Edible Oil Oxidation
WHITE PAPER Early Detection of Edible Oil Oxidation A New Method for the Early Detection of Edible Oil Oxidation Edible oils are used in a wide range of culinary applications. Oils containing unsaturated
More informationHealth effects of consuming 2 portions per week of Scottish farmed salmon raised on different feeding regimes. Baukje de Roos
Health effects of consuming 2 portions per week of Scottish farmed salmon raised on different feeding regimes Baukje de Roos Sustainable sources of fish as food PROGRAMME Aquaculture has the potential
More informationOmega 3s A Key Nutrient for Good Heart Health Laurence Smith, CNC, B.A. 2-On Time Prize Drawing Winners. Omega 3s A Key Nutrient for Good Heart Health
Omega 3s A Key Nutrient for Good Heart Health Download the PDF Handouts at nspwebinars.com 2-On Time Prize Drawing Winners Winners, please email your mailing address and NSP account number to: nspwebinars@natr.com
More informationAmerican Journal of Clinical Nutrition July, 2004;80:204 16
1 Dietary intake of n 3 and n 6 fatty acids and the risk of prostate Cancer American Journal of Clinical Nutrition July, 2004;80:204 16 Michael F Leitzmann, Meir J Stampfer, Dominique S Michaud, Katarina
More information: Overview of EFA metabolism
Figure 1 gives an overview of the metabolic fate of the EFAs when consumed in the diet. The n-6 and n-3 PUFAs when consumed in the form of dietary triglyceride from various food sources undergoes digestion
More informationOmega 3 Fatty Acids and Cardiovascular Disease: An Updated Systematic Review Executive Summary
Evidence Report/Technology Assessment Number 223 Omega 3 Fatty Acids and Cardiovascular Disease: An Updated Systematic Review Executive Summary Introduction Since the first ecological study published in
More informationAnalysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology
Analysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology Claude Mallet, 1 Jennifer Simeone, 2 Paul Rainville 3 1 Workflow Integration Group, Separations Technologies,
More informationThe Triple-Action Power of Omega-3. Three Ways This Essential Nutrient Can Benefit Your Health
The Triple-Action Power of Omega-3 Three Ways This Essential Nutrient Can Benefit Your Health Omega-3 101 You ve heard of them, and you know they re important. But just what are omega-3s, where can you
More informationNational Lipid Association (NLA) May 2017
National Lipid Association (NLA) May 2017 A Phase 1, Single-Dose, Comparative Bioavailability Study of CaPre, a Novel Omega-3 Derived from Krill Oil and Lovaza under Fasting and Fed Conditions Laurent
More informationThe ERA JUMP Study: Reevaluating the Omega- 3 Index
Transcript Details This is a transcript of an educational program accessible on the ReachMD network. Details about the program and additional media formats for the program are accessible by visiting: https://reachmd.com/programs/lipid-luminations/the-era-jump-study-reevaluating-the-omega-3-
More informationSuccessive optimisation of waste cooking oil transesterification in a continuous microwave assisted reactor
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2015 Successive optimisation of waste cooking oil transesterification in a continuous microwave
More informationInfluence of food on the bioavailability of a docosahexaenoic acid supplement
Graduate Theses and Dissertations Graduate College 2012 Influence of food on the bioavailability of a docosahexaenoic acid supplement Stephanie J. Kratzer Iowa State University Follow this and additional
More informationEicosapentaenoic Acid and Docosahexaenoic Acid: Are They Different?
Eicosapentaenoic Acid and Docosahexaenoic Acid: Are They Different? Trevor A Mori, Ph.D., Professor, School of Medicine and Pharmacology, Royal Perth Hospital Unit, University of Western Australia, Perth,
More informationOmega 3s A Key Nutrient for Good Heart Health
4-On Time Prize Drawing Winners Winners, please email your mailing address and NSP account number to: nspwebinars@natr.com Omega 3s A Key Nutrient for Good Heart Health Laurence Smith, CNC, B.A 3 Understanding
More informationApplication note. Enabling purifications of fatty acids with Contichrom
Application note Enabling purifications of fatty acids with Contichrom ChromaCon AG // Purification of fatty acids with Contichrom // www.chromacon.ch // ver. May 2013 1 Overview Fatty acids have broad
More informationOmega-3 requirements - length matters!
Omega-3 requirements - length matters! http://www.rejuvenal.info/ images/terminology/omega3.png Professor Peter Howe Nutritional Physiology Research Centre University of South Australia Food Industry Forum
More information