Shelley Rhodes & Martin Vordermeier
|
|
- Lesley Foster
- 6 years ago
- Views:
Transcription
1 Validation of ante mortem TB tests in Camelids Shelley Rhodes & Martin Vordermeier TB Research Group AHVLA - Weybridge funded by and performed on behalf of the British Alpaca Society, the British Llama Society & British Camelids Ltd (Sept 2010 Sept 2011)
2 TB Research Group AHVLA-Weybridge Shelley Rhodes, Tom Holder & Martin Vordermeier CERA AHVLA-Weybridge Robin Sayers TB Policy AHVLA Ricardo de la Rua-Domenech AHVLA-Langford Lucy Waring Animal Services Unit AHVLA-Weybridge Derek Clifford & Ian Dexter TB Diagnostics LSD AHVLA-Weybridge Jacky Brewer AHVLA-Starcross Tim Crawshaw AHVLA-Worcester Steve Gillgan Chembio Diagnostic Systems, USA Konstantin Lyashchenko IDEXX Reference Laboratories, Canada John Lawrence Laboratory testing, data analysis, reporting Statistical advice and analysis Advice, policy support, coordination Laboratory testing Blood sampling from volunteered animals Bacteriology Post-mortem co-ordination Volunteer herd PM co-ordination Supply of diagnostic kits under development Supply of diagnostic kit under development
3 Definitions: Sensitivity and Specificity Sensitivity The sensitivity of a test is the proportion of truly infected animals that are detected with a test. For example, 90 % sensitivity means that out of 100 infected animals, 90 will be correctly identified (true-positives), and 10 will be missed (false-negatives)
4 Definitions: Sensitivity and Specificity Specificity The specificity of a test is the proportion of truly uninfected animals are correctly classified as test-negative. For example, 90 % specificity means that out of 100 truly negative animals, 90 will be test-negative (true-negatives) whilst 10 will test positive (false-positives)
5 What can ante-mortem diagnostic tests be used for: Herd-level test: Test is designed to detect herds with infection, or to assign disease-free-status to a herd (for example OTF). Examples: Surveillance operations, herd certifications Signature test: Tuberculin skin tests for cattle Individual animal test: Test is designed to detect the maximal number of infected individuals (maximise sensitivity as defined by the OIE and EU directives for cattle). Examples: Clear-up of infected herds to remove sources of infection, premovement trade test, post-movement trade test, tracing test Signature test: Interferon-gamma test used in cattle
6 Summary of TB Immunology: Why different tests can behave differently Tuberculin Skin Test Immune Response Gamma Interferon Test (IFN-γ) Antibody Response Increasing bacterial load Increasing pathology Infectivity
7 1. Validate IFNγ assay AIMS of the study: 2. Compare IFNγ with Serological Tests; STAT-PAK and DPP rapid tests (Chembio, USA) IDEXX ELISA Enferplex ELISA (no data, optimization issues in ROI) Apply tests to; Problem herds (relative Sensitivity) how many truly infected animals identified? (target 50 VL alpacas) TB-free herds (Specificity) how many false-positives do the tests identify (target 300 TB-free alpacas)
8 Tuberculin PPD and defined antigens: Tuberculin is the name given to extracts of Mycobacterium tuberculosis, M. bovis, or M. avium that is used in skin testing in animals and humans to identify a tuberculosis infection. Several types of tuberculin have been used for this, of which purified protein derivative (PPD) is the most important. PPD is a poorly-defined, complex mixture of antigens. An antigen is any substance that causes the immune system to produce antibodies (serology) or T cell responses (IFN-γ, skin test) against it. For bovine tuberculosis diagnosis, bovine and avian PPD is used Bovine PPD is made from M. bovis Avian PPD is made from M. avium We and others have defined components within bovine PPD that are the active components (such as ESAT-6 and CFP-10) that can be synthesised chemically and are therefore defined
9 Test Principle; Interferon-gamma release assay 1. Bleed animals and supply blood to test lab within 24 hours. 2. Culture separated blood lymphocytes in the presence of antigen (PPD-A and PPD-B, ESAT6/CFP10) for 3 days at 37 o C. Antigen (e.g. PPD) IFN-γ T cell Macrophage 3. Harvest cell-free supernatants and measure IFN-γ using ELISA.
10 Sensitivity and Specificity of IFN-γ in cattle (GB data) Diseased animals Cut-off % Sensitivity % Specificity SICCT +ve SICCT -ve and - combined TB-free cattle from the GB Specificity Trial 431 Diseased cattle: 107 SICCT+ : 324 SICCT- *Schiller et al., Clin. Vaccine Immunol., 2009, vol. 16(8):
11 STAT-PAK rapid antibody test (Chembio USA) Dean et al (2009) Veterinary Record Llamas with confirmed Mycobacterium bovis infection Sensitivity of 63.4%, rising to 100% if llamas skin-tested within previous 30 days. Recent study: llamas and alpacas: Sensitivity 71, Specificity 94% (Lyashchenko et al., 2011, CVI 18(12): )
12 DPP (dual path platform, Chembio USA) Martin Vordermeier (TB Research) and Konstantin Lyashchenko (Chembio) Recent published data study: llamas and alpacas: Sensitivity 74%, Specificity 98% (Lyashchenko et al., 2011, CVI 18(12): )
13 IDEXX M. bovis Ab Test (IDEXX) Shelley Rhodes (TB Research), John Lawrence (Idexx) Test developed for cattle, but we have together with IDEXX modified it so that it also works in camelids
14 Animals Diseased/infected: 59 VL alpacas from 10 separate culture-confirmed herds, of which; 55VL were tested with IFNγ 52 were tested with antibody tests 48 were tested with both IFNγ and antibody tests TB-free: 257 alpacas from 17 distinct premises across 9 counties 49 serum samples from USA tested with antibody tests Total of 306 TB-free tested with antibody tests 257 tested with both IFNγ and antibody tests
15 IFNγ Results (55VL & 257 TB-free) 1. Observed results % Sensitivity [95%CI] % Specificity [95%CI] PPD [ ] 89.1 [ ] PPD+ and EC [ ] 98.1 [ ] PPD+ or EC+ 80 [ ] 80.9 [ ] (Cut-offs: PPDB-PPDA>0.1; EC-nil>0.1) 2. Results adjusted for High Specificity % Sensitivity [95%CI] % Specificity [95%CI] PPD [ ] 96.9 [ ] PPD+ and EC [ ] 100 [N/A] PPD+ or EC [ ] 93.8 [ ] (Cut-offs: PPDB-PPDA>0.7272; EC-nil>0.3797)
16 Antibody Test Results (52VL & 306 TB-free) % Sensitivity [95%CI] % Specificity [95%CI] STAT-PAK 67.3 [ ] 97.4 [ ] DPP 57.7 [ ] 96.7 [ ] IDEXX 69.2 [ ] 97.4 [ ] (ROC Cut-offs: DPP>9.05; IDEXX>0.34)
17 IFNγ and Antibody Test Combinations for maximum sensitivity in TB-breakdown herds (48VL & 257 TB-free) 1. Antibody Test Combinations % Sensitivity [95%CI] % Specificity [95%CI] STAT-PAK/IDEXX 81.3 [ ] 95.8 [ ] IDEXX/DPP 72.9 [ ] 94.9 [ ] STAT-PAK/DPP 75 [ ] 94.6 [ ] STAT-PAK/IDEXX/DPP 81.3 [ ] 93 [ ] (ROC Cut-offs: DPP>9.05; IDEXX>0.34)
18 IFNγ and Antibody Test Combinations for maximum sensitivity in TB-breakdown herds (48VL & 257 TB-free) 2. IFNγ and Antibody Test Combinations % Sensitivity [95%CI] % Specificity [95%CI] IFNγ/STAT-PAK 93.8 [87-100] 79.2 [ ] IFNγ/IDEXX 91.7 [ ] 78.6 [ ] IFNγ/DPP 91.7 [ ] 77.4 [ ] IFNγ/STAT-PAK/IDEXX 100 [N/A] 77.8 [ ] IFNγ/STAT-PAK/DPP 100 [N/A] 76.7 [ ] IFNγ/IDEXX/DPP 93.8 [87-100] 75.9 [ ] ROC Cut-offs: IFNγ: PPD+ or EC+ ; PPDB-PPDA>0.1008, EC-nil> DPP>9.05 IDEXX>0.34
19 Summary 1. IFNγ test can be applied with high sensitivity, but one would need to accept lower specificity 2. Application of IFNγ test should depend upon the outcome required e.g. High Sensitivity using PPD or EC option High Specificity using PPD and EC option 3. Animals with M. microti infection will give a positive IFNγ result to PPD 4. Antibody tests can provide high specificity and good sensitivity, but; - they are dependent upon skin test or on animals having severe/advanced pathology - they will not (even in combinations) remove all infected animals 5. Combination of IFNγ and antibody tests maximises detection of infected animals
20 High level summary: 1. More tools in the tool box (= tests of defined performance) 2. Informed/evidence-based policy discussions now possible 3. Evidence that your industry takes btb seriously 4. Application of the blood tests as individual animal tests plays to their strengths: clean-up of infected herds, tracing, trade/movement test Options of possible application in this role (subject to a full appraisal of costs and benefits and discussions between stakeholders and government): Anamnestic StatPak (and/or IDEXX), as a follow-up to the skin test, offers the best combination of Se and Sp for pre-/post-movement TB testing, pre-export testing, and also for tracings from infected herds. To resolve TB outbreaks in known infected herds one could use skin and anamnestic StatPak (or IDEXX) serology as a minimum to lift restrictions, with additional voluntary IFN-γ testing to boost Se, if the herd owner so wishes. 5. Surveillance use: Blood tests are probably not applicable
21 Future work? 1. Define skin tests performances: Single intradermal comparative test, single intradermal test (as potential surveillence/herd test) 2. Add more defined antigens such as ESAT-6/CFP-10 to IFN-γ test to increase the sensitivity of this test in the serial/combination application that has high specificity but low sensitivity: SE SPE PPD+ and EC Define M. microti -specific antigens to allow better differential diagnosis (theoretically possible)
Camelid btb Testing Scenarios
Scenario 1: (Voluntary) Camelid owners undertaking voluntary routine btb surveillance as part of a scheme promoted by the industry and endorsed by Defra. Farm veterinarian referral for blood testing package
More informationBrought to you by: Camelid Industry Presentation on btb Testing 04/09/2015 1
Brought to you by: 1 The information presented here is the culmination of several years work by a team of individuals from the Camelid Industry working with government. It has involved collaboration between
More informationCamelid btb Testing Scenarios Guidance
Department for Environment, Food and Rural Affairs Camelid btb Testing Scenarios Guidance Scenario 1. Camelid owners undertaking voluntary routine btb surveillance as part of a scheme promoted by the industry
More informationBovine TB testing strategy: optimising the use of current diagnostic tools
Bovine TB testing strategy: optimising the use of current diagnostic tools Nigel Gibbens, Chief Veterinary Officer UK NFU bovine TB Science Day, 17 November 2014 Mycobacterium bovis: the basics Member
More informationThe Enferplex TB test and the role of serology in TB diagnosis
The Enferplex TB test and the role of serology in TB diagnosis Alastair Hayton BVM&S DCHP MRCVS RCVS Specialist in Cattle health and Production Synergy Farm Health Ltd, Evershot, UK. Immune responses to
More informationInterferon-gamma blood test What is it and why do we use it?
Interferon-gamma blood test What is it and why do we use it? April 2018 Interferon gamma (IFNγ) test What is it? How does it work? How accurate is it? Why do we use it? When do we use it? IFNγ test Supplementary
More informationBovine TB: the science-policy challenges
Bovine TB: the science-policy challenges Content England s TB strategy The TB problem Controlling the problem What would success look like? Measuring success 2 England s Bovine TB Strategy Aims to: Achieve
More informationP. Dandapat. Eastern Regional Station ICAR-Indian Veterinary Research Institute 37 Belgachia Road, Kolkata
Evaluation of the Sensitivity and Specificity of Gamma Interferon Assay, Single Intradermal Tuberculin and Comparative Intradermal Tuberculin Tests in Naturally Infected Cattle Herds P. Dandapat Eastern
More informationDiagnosis of bovine tuberculosis in Mozambique Comparison of diagnostic methods
Diagnosis of bovine tuberculosis in Mozambique Comparison of diagnostic methods 1 Maxhuza, G., 2 Adelina Machado, A., 3 Manfred Tanner, 4 Irmgard Moser 1 National Department of Veterinary Services, Ministry
More informationCurrent development in the diagnosis of tuberculosis
Current development in the diagnosis of tuberculosis Dr. Lucía de Juan Director of the EURL for Bovine Tuberculosis VISAVET Health Surveillance Centre dejuan@visavet.ucm.es Specific tasks : 1. Maximise
More informationReport of the 2014 USAHA Bovine Tuberculosis Scientific Advisory Subcommittee. Kansas City, Kansas October 20, 2014
Report of the 2014 USAHA Bovine Tuberculosis Scientific Advisory Subcommittee Kansas City, Kansas October 20, 2014 Presentations Integration of Models and Dense Phylogenetic Sampling to Understand BTB
More informationA PRELIMINARY TRIAL TO EVALUATE THE GAMMA- INTERFERON ASSAY FOR THE DETECTION OF TUBERCULOSIS IN CATTLE UNDER LOCAL CONDITIONS IN SERBIA
A PRELIMINARY TRIAL TO EVALUATE THE GAMMA- INTERFERON ASSAY FOR THE DETECTION OF TUBERCULOSIS IN CATTLE UNDER LOCAL CONDITIONS IN SERBIA I. PUŠIĆ 1, VESNA MILIĆEVIĆ 2, SARA SAVIĆ 1, JASNA PRODANOV 1, Ž.GRGIĆ
More informationPRIMAGAM - The primate IFN-ү test
PRIMAGAM - The primate IFN-ү test B. Schroeder {Prionics AG, Schlieren, Switzerland} Correspondence to: Bjoern.Schroeder@prionics.com 1 1 1 1 1 0 1 1 Introduction On December nd 0, Prionics gave a presentation
More informationTB Intensive Tyler, Texas December 2-4, 2008
TB Intensive Tyler, Texas December 2-4, 2008 Interferon Gamma Releasing Assays: Diagnosing TB in the 21 st Century Peter Barnes, MD December 2, 2008 TOPICS Use of interferon-gamma release assays (IGRAs)
More informationIdentifying TB co-infection : new approaches?
Identifying TB co-infection : new approaches? Charoen Chuchottaworn MD. Senior Medical Advisor, Central Chest Institute of Thailand, Department of Medical Services, MoPH Primary tuberculosis Natural history
More informationByrne et al. BMC Veterinary Research (2017) 13:400 DOI /s z
Byrne et al. BMC Veterinary Research (2017) 13:400 DOI 10.1186/s12917-017-1321-z RESEARCH ARTICLE Bovine tuberculosis visible lesions in cattle culled during herd breakdowns: the effects of individual
More informationSerodiagnostics of paratuberculosis Kari R. Lybeck
Serodiagnostics of paratuberculosis Kari R. Lybeck Photo: Birte Graeber Paratuberculosis Paratuberculosis Mycobacterium avium subsp. paratuberculosis (MAP) Chronic, granulomatous enteritis and lymphadenitis
More informationOptions for vaccinating cattle against bovine tuberculosis
Options for vaccinating cattle against bovine tuberculosis Contents 1 Executive summary...4 1.1 Scenarios for use...7 1.1.1 Scenario 1: Compulsory vaccination of all cattle...7 1.1.2 Scenario 2: Compulsory
More informationEvaluation of Mycobacterium tuberculosis specific T cell response to ESAT-6 and PPD antigen with ELISPOT assay
In the name of God Evaluation of Mycobacterium tuberculosis specific T cell response to ESAT-6 and PPD antigen with ELISPOT assay presented by: M. Taheri Symptoms of TB Cough for more than two week Bloody
More informationPoster Abstracts - Diagnosis
- Diagnosis Diagnosis Of Tuberculosis In New World Camelids Infected By Mycobacterium bovis Bezos, J.,, Casal, C., Álvarez, J., Alende, T., Viñolo, C., Sáez, J.L. 4, Domínguez, L., de Juan L., MAEVA SERVET
More informationResearch Article An Evaluation of MAPIA in Michigan as an Ante-Mortem Supplemental Test for Use in Suspect Tuberculosis Cattle
Veterinary Medicine International Volume 2012, Article ID 674368, 6 pages doi:10.1155/2012/674368 Research Article An Evaluation of MAPIA in Michigan as an Ante-Mortem Supplemental Test for Use in Suspect
More informationScientific Opinion on field trials for bovine tuberculosis vaccination 1
EFSA Journal 2013;11(12):3475 SCIENTIFIC OPINION Scientific Opinion on field trials for bovine tuberculosis vaccination 1 EFSA Panel on Animal Health and Welfare (AHAW) 2,3 European Food Safety Authority
More informationTB Nurse Case Management San Antonio, Texas July 18 20, 2012
TB Nurse Case Management San Antonio, Texas July 18 20, 2012 IGRA s and Their Use in TB Nurse NCM Lisa Armitige, MD, PhD July 18, 2012 Lisa Armitige, MD, PhD has the following disclosures to make: No conflict
More informationTB Intensive San Antonio, Texas November 11 14, 2014
TB Intensive San Antonio, Texas November 11 14, 2014 Interferon Gamma Release Assays Lisa Armitige, MD, PhD November 12, 2014 Lisa Armitige, MD, PhD has the following disclosures to make: No conflict of
More informationNew tools for control of bovine tuberculosis in cattle
New tools for control of bovine tuberculosis in cattle Bryce Buddle AgResearch, Hopkirk Research Institute Bovine tuberculosis (TB) in New Zealand Total cost of $100 million/year ($81 million TBfree NZ)
More informationPaper for discussion on Bovine TB in the UK. Written by Dr Ruth Watkins for the ELA.
Paper for discussion on Bovine TB in the UK. Written by Dr Ruth Watkins for the ELA. Introduction Though I was a specialist in Clinical Virology I had a background in General Medicine and Infectious Disease
More informationDevelopment and Evaluation of an Enzyme-Linked Immunosorbent Assay for Use in the Detection of Bovine Tuberculosis in Cattle
CLINICAL AND VACCINE IMMUNOLOGY, Nov. 2011, p. 1882 1888 Vol. 18, No. 11 1556-6811/11/$12.00 doi:10.1128/cvi.05343-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. Development
More informationTB Intensive Houston, Texas October 15-17, 2013
TB Intensive Houston, Texas October 15-17, 2013 Interferon Gamma Release Assays (IGRA s) Lisa Armitige, MD, PhD October 16, 2013 Lisa Armitige, MD, PhD has the following disclosures to make: No conflict
More informationAgencia Española de Medicamentos y Productos Sanitarios C/Campezo 1, Edificio Madrid España (Reference Member State)
DEPARTAMENTO DE MEDICAMENTOS VETERINARIOS Medicamentos y Productos C/Campezo 1, Edificio 8 28022 Madrid España (Reference Member State) MUTUAL RECOGNITION PROCEDURE PUBLICLY AVAILABLE ASSESSMENT REPORT
More informationTB Prevention Who and How to Screen
TB Prevention Who and How to Screen 4.8.07. IUATLD 1st Asia Pacific Region Conference 2007 Dr Cynthia Chee Dept of Respiratory Medicine / TB Control Unit Tan Tock Seng Hospital, Singapore Cycle of Infection
More informationEffect of prolonged incubation time on the results of the QuantiFERON TB Gold In-Tube assay for the diagnosis of latent tuberculosis infection
CVI Accepts, published online ahead of print on 3 July 2013 Clin. Vaccine Immunol. doi:10.1128/cvi.00290-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 2 3 Effect of prolonged
More informationERADICATION OF BOVINE TUBERCULOSIS IN SLOVENIA
ERADICATION OF BOVINE TUBERCULOSIS IN SLOVENIA Presentation for VI International M. bovis Conference Ocepek M., Pate M., Maurer Wernig J., Pirš T. 2,050,000 people 461,500 cattle 63% of the land is covered
More informationESCMID Online Lecture Library. by author
Tuberculosis prevention in immunodepressed patients M. Carmen Fariñas Álvarez Infectious Diseases.H.U.Marqués de Valdecilla University of Cantabria, Spain DISCLOSURES I have no potential conflicts with
More informationEvaluation of an In Vitro Assay for Gamma Interferon Production in Response to Mycobacterium tuberculosis Infections
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, Nov. 2004, p. 1089 1093 Vol. 11, No. 6 1071-412X/04/$08.00 0 DOI: 10.1128/CDLI.11.6.1089 1093.2004 Copyright 2004, American Society for Microbiology. All
More informationDivision of Global Epidemiology, Research Center for Zoonosis Control. Conduct of ELISA test sample collection for the research study in Japan
(Abroad)Official trip report form(student) 13/07/29 Name Laboratory Year (Grade) Destination Marvin Ardeza Villanueva Division of Global Epidemiology, Research Center for Zoonosis Control First Year PhD
More informationApproaches to LTBI Diagnosis
Approaches to LTBI Diagnosis Focus on LTBI October 8 th, 2018 Michelle Haas, M.D. Associate Director Denver Metro Tuberculosis Program Denver Public Health DISCLOSURES I have no disclosures or conflicts
More informationDevelopment of a DIVA Skin-Test for Bovine Tuberculosis. Division, Stormont, Belfast, BT4 3SD, Northern Ireland.
JCM Accepts, published online ahead of print on 30 June 2010 J. Clin. Microbiol. doi:10.1128/jcm.00420-10 Copyright 2010, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationCompetent Authority comments on the draft report received 2 March 2018
Competent Authority comments on the draft report received 2 March 2018 1. (p6) After Paragraph No.1, we would like to add a paragraph about National Institute of Animal Health (NIAH), shown below, because
More informationSurveillance and Schmallenberg Virus Alasdair Cook MRCVS, AHVLA. Sheep Health & Welfare Conference Worcester, 21 st November 2012
Surveillance and Schmallenberg Virus Alasdair Cook MRCVS, AHVLA Sheep Health & Welfare Conference Worcester, 21 st November 2012 AHVLA: Supporting a healthy and sustainable food and farming industry across
More informationInquiry examining the vaccination of badgers and cattle in relation to Bovine TB
Inquiry examining the vaccination of badgers and cattle in relation to Bovine TB A response from the Society of Biology to the Environment, Food and Rural Affairs Committee 7 January 2013 The Society of
More informationUsing Interferon Gamma Release Assays for Diagnosis of TB Infection
Learning Objectives Using Interferon Gamma Release Assays for Diagnosis of TB Infection 1. Describe available Interferon Gamma Release Assay tests for TB infection and how they work. 2. Understand interpretation
More informationSERVAL: A generic framework for the evaluation of animal health surveillance
SERVAL: A generic framework for the evaluation of animal health surveillance Incorporating a worked example from a case study of pre-movement testing for bovine tuberculosis in England and Wales Developed
More informationField Evaluation of the Efficacy of Mycobacterium bovis Bacillus Calmette-Guérin against Bovine Tuberculosis in Neonatal Calves in Ethiopia
CLINICAL AND VACCINE IMMUNOLOGY, Oct. 2010, p. 1533 1538 Vol. 17, No. 10 1556-6811/10/$12.00 doi:10.1128/cvi.00222-10 Copyright 2010, American Society for Microbiology. All Rights Reserved. Field Evaluation
More informationHungary Report Dr Lajos Bognár
Standing Group of Experts on Lumpy Skin Disease in Europe under the GF-TADs umbrella First meeting (LSD1) Brussels, Belgium, 4-5 July 2016 Hungary Report Dr Lajos Bognár Enabling environment in place Legal
More informationCHILDHOOD TUBERCULOSIS: NEW WRINKLES IN AN OLD DISEASE [FOR THE NON-TB EXPERT]
CHILDHOOD TUBERCULOSIS: NEW WRINKLES IN AN OLD DISEASE [FOR THE NON-TB EXPERT] QUESTION: : Which children in the United States should get a tuberculin skin test? Do questionnaires really work? Jeffrey
More informationWorking Document. Eradication of Bovine Tuberculosis in the EU. accepted by the Bovine tuberculosis subgroup of the
EUROPEAN COMMISSION HEALTH & CONSUMER PROTECTION DIRECTORATE-GENERAL Unit 04 -Veterinary Control Programmes. Brussels, 10/08/2006 SANCO/10200/2006 final Working Document on Eradication of Bovine Tuberculosis
More informationBovine Brucellosis Control of indirect ELISA kits
Bovine Brucellosis Control of indirect ELISA kits (Individual serum samples & pooled serum samples) Standard Operating Procedure Control of Bovine brucellosis Serum ELISA kits SOP Page 1 / 7 02 February
More informationVariation in T-SPOT.TB spot interpretation between independent observers of different laboratories
8 Variation in T-SPOT.TB spot interpretation between independent observers of different laboratories Willeke P.J. Franken 1, Steven Thijsen 2, Ron Wolterbeek 3, John J.M. Bouwman 2, Hanane el Bannoudi
More informationEvaluation and Treatment of TB Contacts Tyler, Texas April 11, 2014
Evaluation and Treatment of TB Contacts Tyler, Texas April 11, 2014 Interferon Gamma Release Assays: Understanding the Test David Griffith, BA, MD April 11, 2014 David Griffith, BA, MD has the following
More informationTesting for TB. Bart Van Berckelaer Territory Manager Benelux. Subtitle
Testing for TB Bart Van Berckelaer Territory Manager Benelux Subtitle Agenda TB infection pathway TB immunisation Testing options Pre lab considerations of the whole blood ELISA test The T-SPOT.TB test
More informationU.S. Breeding Cattle Exports to Vietnam Are Approved
THIS REPORT CONTAINS ASSESSMENTS OF COMMODITY AND TRADE ISSUES MADE BY USDA STAFF AND NOT NECESSARILY STATEMENTS OF OFFICIAL U.S. GOVERNMENT POLICY Voluntary - Public Date: 7/11/2011 GAIN Report Number:
More informationDidactic Series. Latent TB Infection in HIV Infection
Didactic Series Latent TB Infection in HIV Infection Jacqueline Peterson Tulsky, MD UCSF Positive Health Program at SFGH Medical Director, SF and North Coast AETC March 13, 2014 ACCREDITATION STATEMENT:
More informationIdentification of novel antigens recognized by serum antibodies in bovine tuberculosis
CVI Accepted Manuscript Posted Online 4 October 2017 Clin. Vaccine Immunol. doi:10.1128/cvi.00259-17 Copyright 2017 American Society for Microbiology. All Rights Reserved. 1 Identification of novel antigens
More informationMaking the Diagnosis of Tuberculosis
Making the Diagnosis of Tuberculosis Alfred Lardizabal, MD NJMS Global Tuberculosis Institute Testing for TB Infection Targeted Testing: Key Points Test only if plan for ensuring treatment De-emphasizes
More informationNATIONAL INSTITUTE FOR HEALTH AND CLINICAL EXCELLENCE SCOPE
TB Partial Update Appendix 1 - Scope NATIONAL INSTITUTE FOR HEALTH AND CLINICAL EXCELLENCE 1 Guideline title SCOPE Tuberculosis: interferon gamma tests for the diagnosis of latent tuberculosis (partial
More informationNATIONAL INSTITUTE FOR HEALTH AND CLINICAL EXCELLENCE SCOPE
NATIONAL INSTITUTE FOR HEALTH AND CLINICAL EXCELLENCE 1 Guideline title SCOPE Tuberculosis: interferon gamma tests for the diagnosis of latent tuberculosis (partial update) 1.1 Short title Tuberculosis
More informationReceived 3 August 2011/Returned for modification 22 August 2011/Accepted 28 September 2011
CLINICAL AND VACCINE IMMUNOLOGY, Dec. 2011, p. 2154 2160 Vol. 18, No. 12 1556-6811/11/$12.00 doi:10.1128/cvi.05329-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. Serum Antibody
More informationTechnical Bulletin No. 172
CPAL Central Pennsylvania Alliance Laboratory QuantiFERON -TB Gold Plus Assay Contact: J Matthew Groeller, MPA(HCM), MT(ASCP), 717-851-4516 Operations Manager, Clinical Pathology, CPAL Jennifer Thebo,
More informationPerspective in novel TB vaccine development Mohamed Ridha BARBOUCHE M.D., Ph.D. Department of Immunology Institut Pasteur de Tunis
Perspective in novel TB vaccine development Mohamed Ridha BARBOUCHE M.D., Ph.D. Department of Immunology Institut Pasteur de Tunis Existing TB Vaccine is not effective for global TB epidemic control BCG
More informationKristin Elaine Bass Iowa State University
Graduate Theses and Dissertations Graduate College 2013 The evaluation of Mycobacterium tuberculosis complex species-specific antigens and purified protein derivatives for the detection of bovine tuberculosis
More informationNew NICE guideline updates recommendations for diagnosing latent tuberculosis
Tel: 0845 003 7782 www.nice.org.uk Ref: 2011/053 PRESS RELEASE New NICE guideline updates recommendations for diagnosing latent tuberculosis The National Institute for Health and Clinical Excellence (NICE)
More informationA Clinician s Perspective: Improving Rheumatology Patient Care Using the T-SPOT.TB Test
A Clinician s Perspective: Improving Rheumatology Patient Care Using the T-SPOT.TB Test Solomon Forouzesh, MD, FACD, FACR Medical Director Arthritis Care & Treatment Center Clinical Associate Professor
More informationBovine Tuberculosis Disease Control. Tuesday 20th May 2013, pm, Jubilee Room, Westminster Hall
Bovine Tuberculosis Disease Control Tuesday 20th May 2013, 2.00 4.00pm, Jubilee Room, Westminster Hall Seminar Summary Badgers are a significant source of bovine tuberculosis (BTB), a bacterial disease
More informationTB, BCG and other things. Chris Conlon Infectious Diseases Oxford
TB, BCG and other things Chris Conlon Infectious Diseases Oxford Epidemiology Latent TB IGRA BCG >50/100000
More informationMadhukar Pai, MD, PhD Jessica Minion, MD
Optimism bias, inflated accuracy estimates, and contradicted findings in TB diagnostic research Madhukar Pai, MD, PhD [madhukar.pai@mcgill.ca] Jessica Minion, MD McGill University, Montreal 1 Context There
More informationDiagnostic Value of ELISPOT Technique for Osteoarticular Tuberculosis
Clin. Lab. 2014;60:1865-1870 Copyright ORIGINAL ARTICLE Diagnostic Value of ELISPOT Technique for Osteoarticular Tuberculosis XUEQIONG WU 1, *, YUANZHENG MA 2, *, LAN WANG 1, DAWEI LI 2, YOURONG YANG 1,
More informationReview. Interferon- assays in the immunodiagnosis of tuberculosis: a systematic review. Interferon- assays for tuberculosis diagnosis
Interferon- assays for tuberculosis diagnosis Review Interferon- assays in the immunodiagnosis of tuberculosis: a systematic review Madhukar Pai, Lee W Riley, and John M Colford Jr A major challenge in
More informationImproving surveillance Improving management of high risk herds Supporting studies... 24
bovine Tuberculosis 8 Biennial Report, 2008/2009 Bovine tuberculosis bovine Tuberculosis Tuberculosis in cattle Improving surveillance... 0 Improving management of high risk herds... 6 Supporting studies...
More informationPestivirus what might it mean to your herd?
Pestivirus what might it mean to your herd? Smith, A.K. & Allworth, M.B. What do we know about Bovine Pestivirus? Bovine Pestivirus = Bovine Viral Diarrhoea virus (BVDV) Occurs worldwide Been around for
More informationPeggy Leslie-Smith, RN
Peggy Leslie-Smith, RN EMPLOYEE HEALTH DIRECTOR - AVERA TRAINING CONTENT 1. South Dakota Regulations 2. Iowa Regulations 3. Minnesota Regulations 4. Interferon Gamma Release Assay (IGRA)Testing 1 SOUTH
More informationOIE Reference Laboratory Reports Activities
OIE Reference Laboratory Reports Activities Activities in 2017 This report has been submitted : 2018-03-19 14:06:55 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Bovine
More informationAnimal Health Requirements for poultry meat etc. to be exported to Japan from Finland
Animal Health Requirements for poultry meat etc. to be exported to Japan from Finland Animal Health requirements for poultry meat etc. (hereinafter referred to as the exported poultry meat etc. ) are applied
More informationSpecific Delayed-Type Hypersensitivity Responses to ESAT-6 Identify Tuberculosis-Infected Cattle
JOURNAL OF CLINICAL MICROBIOLOGY, May 2003, p. 1856 1860 Vol. 41, No. 5 0095-1137/03/$08.00 0 DOI: 10.1128/JCM.41.5.1856 1860.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved.
More informationAnimal TB Research Group
1ANIMAL TB RESEARCH GROUP STELLENBOSCH UNIVERSITY Animal TB Research Group Stellenbosch University NEWSLETTER 2014-2015 IN THIS ISSUE Animal TB Research Group December 2015 The Stellenbosch University
More informationEXPORT OF IN-VIVO DERIVED OVINE AND CAPRINE EMBRYOS TO NEW ZEALAND
DEPARTMENT FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS SCOTTISH GOVERNMENT WELSH GOVERNMENT DEPARTMENT OF AGRICULTURE, ENVIRONMENT AND RURAL AFFAIRS - NORTHERN IRELAND EXPORT OF IN-VIVO DERIVED OVINE AND CAPRINE
More informationDidactic Series. Latent TB Infection in HIV Infection
Didactic Series Latent TB Infection in HIV Infection Jacqueline Peterson Tulsky, MD UCSF Positive Health Program at SFGH Medical Director SF, North Coast and East Bay AETC January 8, 2015 ACCREDITATION
More informationLIAISON QuantiFERON -TB Gold Plus Solution
Tuberculosis LIAISON QuantiFERON -TB Gold Plus Solution Let TB testing flow FOR OUTSIDE THE US AND CANADA ONLY LIAISON QuantiFERON -TB Gold Plus Solution A new fully automated CLIA assay to aid in the
More informationIn our paper, we suggest that tuberculosis and sarcoidosis are two ends of the same spectrum. Given the pathophysiological and clinical link between
In our paper, we suggest that tuberculosis and sarcoidosis are two ends of the same spectrum. Given the pathophysiological and clinical link between the two, we also propose a classification system for
More informationImport Health Standard. For. Bovine Embryos
Import Health Standard For Bovine Embryos Short Name: bovemid.gen Ministry of Agriculture and Forestry P.O Box 2526 Wellington 6011 New Zealand BOVEMID.GEN 27 June 2011 Page 1 of 13 Issuing Authority This
More informationTuberculosis and Johne s Disease
Tuberculosis and Johne s Disease Prof. R. S. Chauhan Joint Director, Indian Veterinary Research Institute Izatnagar 243 122 (India) TUBERCULOSIS Tuberculosis is a chronic bacterial disease of animals caused
More informationDetecting latent tuberculosis using interferon gamma release assays (IGRA)
Detecting latent tuberculosis using interferon gamma release assays (IGRA) American Society for Microbiology June 2017 Edward Desmond, Ph.D., D (ABMM) San Lorenzo, CA Edward Desmond has no financial connections
More informationMycobacterial Infections: What the Primary Provider Should Know about Tuberculosis
Mycobacterial Infections: What the Primary Provider Should Know about Tuberculosis Henry F. Chambers, M.D Professor of Medicine, UCSF Topics for Discussion Epidemiology Diagnosis of active TB Screening
More informationARTICLE IN PRESS. Preventive Veterinary Medicine xxx (2010) xxx xxx. Contents lists available at ScienceDirect. Preventive Veterinary Medicine
Preventive Veterinary Medicine xxx (2010) xxx xxx Contents lists available at ScienceDirect Preventive Veterinary Medicine journal homepage: www.elsevier.com/locate/prevetmed Approaches towards optimising
More informationComparison of Tuberculin Skin test and Quantiferon immunological assay for latent Tuberculosis infection
Monaldi Arch Chest Dis 2005; 63: 3, 158-162 TB CORNER Comparison of Tuberculin Skin test and Quantiferon immunological assay for latent Tuberculosis infection L.R. Codecasa 1, M. Ferrarese 1, V. Penati
More informationMAXIMISING EFFICIENCY WITH A SURVEILLANCE STRATEGY FOR FOOT-AND-MOUTH DISEASE DURING AN OUTBREAK IN A PREVIOUSLY FMD-FREE COUNTRY.
MAXIMISING EFFICIENCY WITH A SURVEILLANCE STRATEGY FOR FOOT-AND-MOUTH DISEASE DURING AN OUTBREAK IN A PREVIOUSLY FMD-FREE COUNTRY. Kylee Walker Incursion Investigator and Veterinary Epidemiologist, Ministry
More informationSummary of Immunization Options
Student Health Services 30 Bee Street Suite 102 Charleston, SC 29425 Telephone 843-792-3664 Fax 843-792-2569 Visiting Students Immunization Requirements All MUSC students, including visiting students,
More information2016 OPAM Mid-Year Educational Conference, Sponsored by AOCOPM Sunday, March 13, 2016
Learning Objectives Tuberculosis Case Discussions: Evaluation for Tuberculosis Infection Melissa C. Overman, DO, MPH, CHES, FAOCOPM Describe appropriate technique for TST placement, reading and interpretation
More informationExport Requirements Notification - Animal Products Act Standards Branch, Animal and Animal Products Directorate, Ministry for Primary Industries
Export Requirements Notification - Animal Products Act 1999 Standards Branch, Animal and Animal Products Directorate, Ministry for Primary Industries Ref: AE-UK-45L Date: 11 March 2014 ZOOPENEC.UK 13 MARCH
More informationHPAI H5N8 Sweden 2016
HPAI H5N8 Sweden 2016 Swedish Board of Agriculture, Jönköping, Sweden Outbreak Summary 2016-12-01 21 November 2016: Increased mortality noted. 22 November: Private vet reports suspicion of ND or AI, following
More informationTable 9. Policy for Tuberculosis Surveillance and Screening
Policy for Tuberculosis Surveillance and Screening Purpose: to identify active cases of tuberculosis or latent TB among residents and staff of the nursing home in order to prevent transmission in this
More informationIGRA Test Reliability. How Test Design and Lab Control Impact Results
IGRA Test Reliability How Test Design and Lab Control Impact Results IGRA Test Reliability Background Why IGRAs are uniquely challenging to both test manufacturers and labs Why complexity exists with both
More informationChirilă COVALIU. PhD coordonator, Prof. univ. dr. Tudor PERIANU. - I a s s y
UNIVERSITY AGRICULTURAL SCIENTE AND VETERINARY MEDICINE ION IONESCU DE LA BRAD IASSY FACULTY OF VETERINARY MEDICINE DOMAIN VETERINARY MEDICINE SPECIALITY INFECTIOUS DISEASE Chirilă COVALIU D O C T O R
More informationAntigen Mining with Iterative Genome Screens Identifies Novel Diagnostics for the Mycobacterium tuberculosis Complex
CLINICAL AND VACCINE IMMUNOLOGY, Jan. 2006, p. 90 97 Vol. 13, No. 1 1556-6811/06/$08.00 0 doi:10.1128/cvi.13.1.90 97.2006 Copyright 2006, American Society for Microbiology. All Rights Reserved. Antigen
More informationReceived 27 October 2005/Returned for modification 27 December 2005/Accepted 12 January 2006
CLINICAL AND VACCINE IMMUNOLOGY, Mar. 2006, p. 387 394 Vol. 13, No. 3 1556-6811/06/$08.00 0 doi:10.1128/cvi.13.3.387 394.2006 Copyright 2006, American Society for Microbiology. All Rights Reserved. Effects
More informationImportance of cell mediated immunity for protection against Foot and Mouth Disease
Appendix 34 Importance of cell mediated immunity for protection against Foot and Mouth Disease Yooni Oh 1*, ryan Charleston 1, David J. Paton 1, Jong-Hyun Park 2, Paul V. arnett 1, Yi-Seok Joo 2, Satya
More informationUtility of PPD or IGRA to answer the age old question of "TB or not TB
Utility of PPD or IGRA to answer the age old question of "TB or not TB Thomas S. Alexander, Ph.D., D(ABMLI) Immunologist Summa Health alexandt@summahealth.org Yes, The reservation is in the name of Dr.
More informationUse of Interferon-γ Release Assays (IGRAs) in TB control in low and middle-income settings - EXPERT GROUP MEETING -
Use of Interferon-γ Release Assays (IGRAs) in TB control in low and middle-income settings - EXPERT GROUP MEETING - Date and time: 20-21 July 2010, 09:00 18:00 Venue: Salle B, WHO-HQ, Geneva, Switzerland
More informationDEPARTMENT OF THE ARMY OFFICE OF THE SURGEON GENERAL 5109 LEESBURG PIKE FALLS CHURCH, VA
DEPARTMENT OF THE ARMY OFFICE OF THE SURGEON GENERAL 5109 LEESBURG PIKE FALLS CHURCH, VA 22041-3258 DASG-PPM-NC r% 5 SEP 2008 MEMORANDUM FOR SEE DISTRIBUTION SUBJECT: Supplemental guidance for the Army
More informationDevelopment and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis
Risalde et al. BMC Veterinary Research (2017) 13:341 DOI 10.1186/s12917-017-1262-6 RESEARCH ARTICLE Open Access Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis
More informationBovine TB: Epidemiology and Ecology of a Multi-Host Disease
Bovine TB: Epidemiology and Ecology of a Multi-Host Disease Christl Donnelly MRC Centre for Outbreak Analysis and Modelling Department of Infectious Disease Epidemiology Imperial College London (and in
More information