Comparison of insecticide efficacies against BMSB adults Regional BMSB insecticide bioassays project 2017 summary

Transcription

1 Comparison of insecticide efficacies against BMSB adults Regional BMSB insecticide bioassays project 17 summary George Hamilton (Rutgers U.), Peter Jentsch (Cornell U.), Greg Krawczyk (PSU), Tom Kuchar (VA Tech), Jim Walgenbach (NC State) and Celeste Welty (OSU). This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Specialty Crop Research Initiative under award number : Management of BMSB in US Specialty Crops

2 Evaluations of insecticide efficacies against BMSB (PSU 1-11)

3 Most effective insecticides against BMSB (based on combined data from T. Leskey, T. Kuchar and G. Krawczyk) PYRETHROIDS IRAC Group 3A NEONICOTINOIDS IRAC Group 4A OTHER (IRAC Group 1A, ) bifenthrin (Brigade) fenpropathrin (Danitol) cyfluthrin (Baythroid) λ-cyhalothrin (Warrior) dinotefuran (Venom, Scorpion) thiametoxam (Actara) clothianidin (Belay) imidacloprid (Provado, Admire Pro) acetamiprid (Assail) methomyl (carbamate) (Lannate LV and SP)

4 17 BMSB Insecticide resistance testing: Methods Product Rate Max field rate bifenthrin (Bifenture EC).13 ml/1ml 12.8 fl oz/ac thiametoxam (Actara).41 g/1ml 5.5 oz/ac Tested insecticide concentrations are equivalents of 1%, 25%, 5% and 1% of recommended full field rate

5 Insecticide activity against BMSB Direct contact topical bioassays - 17 Subject Field collected BMSB adults Geographically distinct populations (OH, NC, NJ, NY, PA, VA) Male (n=1 - ) and female (n=8 - ) tested separately Test Commercial grade insecticide solutions at equivalent of max label based field rate (1 gal/acre), surfactant added; Each individual bug treated directly with 2 μl of solution Results Mortality assessed at 3, 24 and 48 hours after treatment Response categories live, dead, moribund

6 % Live % Dead BMSB bifenthrin bioassays 17 Males vs females comparison all BMSB populations combined 3 Hours 24 Hours 48 Hours Male Female Hours 24 Hours 48 Hours Male Female Application Rate (Proportion of max field rate of 12.8 fl oz/ac)

7 Percent BMSB Percent BMSB BMSB bifenthrin bioassays 17 Individual BMSB populations the moribund category dilemma PA BMSB population Control.1x.25x.5x 1x Dead Moribund Live VA BMSB population Control.1x.25x.5x 1x Dead Moribund Live Mortality readings ATT (hours)

8 Live plus Moribund (%) Dead + Moribund (%) BMSB bifenthrin bioassays 17 Males vs females comparison all BMSB populations combined 1 3 Hours 1 24 Hours 1 48 Hours Male Female Hours 24 Hours 48 Hours Male Female Application Rate (Proportion of max field rate of 12.8 fl oz/ac)

9 Dead + moribund (%) BMSB bifenthrin bioassays 17 Comparison of BMSB populations males and females combined hours 48 hours ns * * * * ns * * * * NC OH VA PA NC OH VA PA 1 * - significant at P.5 (ANOVA, Fisher s Protected LSD, sqrt transformation)

10 14 BMSB insecticide resistance testing: bifenthrin (Bifenture EC ) (dead plus moribund BMSB adults) * 3 h AT 24 h AT 48 h AT * * ns ns ns ns * ns * - significant at P.5 (ANOVA, Fisher s Protected LSD, arcsin transformation)

11 Live (%) Dead (%) BMSB thiametoxam bioassays 17 Males vs females comparison all BMSB populations combined Hours Hours Hours Hours Hours 48 Hours Male Female Male Female Application Rate (Proportion of max field rate of 5.5 oz/ac)

12 Percent BMSB Percent BMSB BMSB thiametoxam bioassays 17 Individual BMSB populations the moribund category dilemma PA BMSB population 1 Control.1x.25x.5x 1x 8 6 Dead Moribund Live Control.1x VA BMSB population.25x.5x 1x Dead Moribund Live Mortality readings ATT (hours)

13 Live plus Moribund (%) Dead + Moribund (%) BMSB thiametoxam bioassays 17 BMSB males vs females comparison all populations combined 3 Hours 24 Hours 48 Hours Male Female Hours 24 Hours 48 Hours Male Female Application Rate (Proportion of max field rate of 5.5 oz/ac)

14 Dead + moribund (%) BMSB thiametoxam bioassays 17 Comparison of BMSB populations males and females combined hours 48 hours ns * * ns ns ns * * * * NC OH VA PA NC OH VA PA 1 * - significant at P.5 (ANOVA, Fisher s Protected LSD, sqrt transformation)

15 BMSB adults topical bioassays 24 hours 48 hours Alive Moribund Dead 72 hours 7 days Alive Moribund Dead Alive Moribund Dead Alive Moribund Dead Hudson Valley Research Laboratory

16 Summary Products recommended for the BMSB management such as Bifenture or Actara are effective against BMSB adults. The differences in BMSB male and female responses to various products was not significant, although it appears to be size related Three evaluated BMSB populations from OH, PA and VA responded similarly to tested products, while the NC population was less sensitive. The moribund response of treated BMSB adults during a topical bioassay influenced the mortality readings in the laboratory bioassays, however it should be treated positively for the overall efficacy of the product during a field application.

17 THANK YOU!