Faculty of Biological Sciences COSHH Assessment

Transcription

1 Faculty of Biological Sciences COSHH Assessment Brief Description of Activity, Experiment or Process Assessment Title Tissue culture Area/Service Protein Production Facility PI(s) or Manager(s) in charge Dr Brian Jackson Assessment Code Basic Tissue culture of primary cells or cell lines, passage of cells and use of common antibiotics PPF09 Date: July 2016 Safety Overview In addition to Good Lab Practice the following controls are required within this experiment (refer to details below for further information) Control Fume Hood Other Ventilation Spill Tray Control of aerosols Gloves* Glasses/visor Protective Clothing* Other (give brief details) Tick *additional to measures taken or PPE worn when working within Good Lab Practice e.g. thick chemical resistant gloves, apron etc. Emergency Information detail how foreseeable emergencies based on risk from chemical would be dealt with (fill this section in once assessment is complete) Examples: Chemical EDTA Trypsin Papain Penicillin Streptomycin Trypsin inhibitor Flammable chemicals extinguishing if the quantities involved in the work caught fire Corrosive chemicals first aid measures/actions if there is with eyes or skin Chemicals dangerous by inhalation fire aid/actions if worker accidentally inhaled Chemicals dangerous if swallowed first aid/actions if worker ingests accidently Action in foreseeable emergency General advice In case of skin : Take off contaminated clothing and shoes immediately. Wash off with soap and plenty of water. Take casualty to a shower if severity warrants it and H&S for advice and potential treatment with Diphoterine. In case of eye : Rinse thoroughly with plenty of water for at least 20 minutes If swallowed: Do NOT induce vomiting. Rinse mouth with water if conscious. If inhaled: Move person into fresh air. If not breathing, give artificial respiration. Summon ambulance or take casualty to A&E if effects of any are not relieved by simple first aid measures. Ethanol Firefighting measures Extinguishing media Suitable extinguishing media Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Special s arising from the substance or mixture Carbon oxides Brief Overview No symbol assigned (+0) Labelling/Pictograms of chemicals involved in Process (+1) (+3) (+3) (+3) Signal Words (+0) Refer to Guidance Tick all that Apply No Hazard Health Hazard Corrosive Flammable Oxidiser Explosive Serious Health Hazard Toxic Gas Cylinder Harmful in Environment

2 Chemical Name and CAS no. Ethanol EDTA (usually within Trypsin buffer) Trypsin Papain Trypsin inhibitor Penicillin Streptomycin Hygromycin B Hazard Labels and Signal Words and specific effect (if applicable) e.g. Damage to respiratory system if inhaled Highly flammable liquid and vapour Causes serious eye irriation Causes skin irritation Causes eye irritation May cause allergy or asthmatic symptoms May cause respiratory irritation Causes Skin irritation May cause allergy or asthmatic symptoms May cause respiratory irritation May cause allergy or asthma symptoms if inhaled May cause sensitisation by inhalation and skin May cause allergic skin reaction May cause allergy or asthma symptoms if inhaled Harmful if swallowed Possible risk of harm to unborn child Fatal if Swallowed, in with skin or inhaled Causes serious eye damage May cause allergic or asthmatic symptoms Chemicals Hazards Classification (1-5) Scoring of s presented within experiment - Does the experiment pose a ous exposure? A B C D E F G H Hazardous vapour or fumes (+3) Dusty/light powders Aerosols generated Splashes or drips likely (+1) Skin or eye likely Ignition source may cause fire Carcinogen, mutagen, toxin or teratogen (+5) Other Hazard (assign score 1-3 based on severity) (e.g. working above WEL*) 4 yes yes yes yes yes TWA 1000ppm 14 1 yes yes yes yes 8 5 Yes Yes Yes Yes Serious 5 Yes Yes Yes Yes Serious 3 yes yes yes yes 10 Total Haza rd (1-25) The following chemicals used in the procedure are not considered ous Media (Dulbecco s MEM), L-glutamine, Foetal bovine serum, Balanced salt solution (Hank s), HBSS (CMF) Hazardous vapour or fumes (+3) Dusty/light powders Aerosols generated Splashes or drips likely (+1) Skin or eye likely Ignition source may cause fire Carcinogen, mutagen, toxin or teratogen (+5) Other Hazard (assign score 1-3 based on severity) (e.g. working above WEL*) A B C D E F G H Total Haza rd (1-25)

3 *WEL = Work place exposure limit. Detailed in HSE document EH Control identification and Specification Give details of controls/procedures required in addition to Good Lab Practice to avoid or reduce exposure to s (e.g. identify stages where a control is needed, specific controls for one chemical etc). Be as specific as possible e.g. perform process A using chemical X in fume hood; weight out chemical Y powder in chemical cabinet; pipette chemical W very gently to reduce aerosol; pipette (do not pour) chemical Z from stock bottle. s are reduced by deducting score allocated above where exposure is successfully controlled. is only partially reduced for control by PPE only (+1 to remain). Record final residual score in table below. Chemical: Ethanol Controlled? Sc. Chg. Chemical: Trypsin-EDTA Hazards: A C D E F H (delete as required) Hazards: B C D E G (delete as required) A Exposure to vapour likely because ethanol is sprayed. Always spray away from self and contain within culture cabinet where possible. C Pipette liquids instead of pouring when making up stocks. Seal container when not in use. D&E Always use gloves when handling liquid. Seal container outside of F/C. D&E Contain within culture hood. Always use gloves when handling. Wear lab coat. F: Ensure ignition sources are removed when pouring from stock bottle. Do not spray G Possibility of serious effects such as sensitisation and asthma. Controlled as near open flame. H Assigned exposure limit noted. Not expected to be exceeded in normal use. Yes 0 Monitoring available 9 12 Chemical: Papain Controlled? Sc. Chg. Chemical: Trypsin inhibitor Hazards: B C D E G (delete as required) Hazards: B C D E G (delete as required) D&E Contain within culture hood. Always use gloves when handling. Wear lab coat. D&E Contain within culture hood. Always use gloves when handling. Wear lab coat. G Possibility of serious effects such as sensitisation and asthma. Controlled as G Possibility of serious effects such as sensitisation and asthma. Controlled as Chemical: penicillin Controlled? Sc. Chg. Chemical: Streptomycin Hazards: B C D E G (delete as required) Hazards: B C D E G (delete as required) D&E Contain within culture hood. Always use gloves when handling. Wear lab coat. D&E Contain within culture hood. Always use gloves when handling. Wear lab coat. G Possibility of serious effects such as sensitisation and asthma. Controlled as G Possibility of serious effects such as potential harm to an unborn child. Controlled as outlined above. Concentration is low and liquid typically used in small quantities and contained within a tissue culture hood. 12 7

4 Chemical: Hygromycin Hazards: B C D E G (delete as required) D&E Contain within culture hood. Always use gloves when handling. Wear lab coat. G Possibility of serious effects such as sensitisation and asthma. Controlled as Controlled? Sc. Chg. 12 Residual Risk from Chemicals in Experiment If a ous exposure is adequately controlled then Hazard from chemical identification above can be reduced accordingly and recorded below. cannot be reduced below Classification Highlight scores greater than 12 in Red or Bold/Underline) Chemical Haz. Sc. Change Residual Risk Chemical Haz Sc. Change Residual Risk Ethanol Penicillin 12 5 Trypsin 12 5 Hygromycin 12 5 Papain 12 5 Streptomycin Trypsin inhibitor 12 5 If residual risk score is still greater than 12 then Appendix A must be completed to ensure specific controls for the chemical are specified and put in place Waste Disposal Detail how waste resulting from this work will be disposed of No Solid Waste: Can all solid waste be disposed of into Healthcare waste or autoclave waste as appropriate to biological agent contamination If no, give details below Liquid Waste: Can all liquid waste be disposed of into clinical waste or autoclave waste as appropriate to biological agent contamination Yes If no, give details below Environmental Hazards: Are any chemicals used in this work labelled as environmental s? These must not be placed into the drain. Specific measures required for waste containing chemicals not suitable for disposal to care waste or to the drain. Chemical (e.g. Chloroform) State of waste (e.g. liquid, contaminated solids etc) Disposal Route (chemical waste (solvent) route for liquid and cin bin for solids) Notes about disposal (e.g. remove solvent waste to Miall waste store) All Solid powder/liquids Specialist Contactor Once diluted to working strength liquids can be disposed of down the sink. However, neat or unused antibiotics and dissociation reagents must be disposed of by contractor No If YES, detail below Risk assessment Approval COSHH Assessment must be approved by the Academic(s)/Manager(s) in charge of the work before it becomes valid The risk assessment approver must ensure that staff and students carrying out the work assessed here are provided with adequate supervision to ensure they are carrying out the procedures stipulated. Name of Manager/Academic in charge Signature Date Dr Brian R Jackson 19/07/2016

5