CHAPTER 4 IMMUNOLOGICAL TECHNIQUES
|
|
- Todd Neal
- 5 years ago
- Views:
Transcription
1 CHAPTER 4 IMMUNOLOGICAL TECHNIQUES
2 Nitroblue Tetrazolium Chloride (NBT) Reduction test NBT reduction test was evaluated by employing the method described by Hudson and Hay,1989 based upon principle that whenever a particle is ingested by a phagocyte, a respiratory burst is induced. This test is employed to measure the macrophage/neutrophil function. The assay is based on the reaction that the addition of yellow color NBT dye to splenocyte suspension results in the formation of complex which can be phagocytosed by neutrophils/macrophages. The yellow colored NBT is reduced to blue colored formazone which can be extracted in dioxin/pyridine and hence measured spectrophotometrically at 520 nm using dioxan as blank. 1. Phosphate Buffer Saline (PBS) 0.15 M, ph = mm NBT in 0.15 M PBS containing 34 mm sucrose N HCl 4. Dioxin: Commercially available (Ranbaxy Pvt. Ltd.) Briefly, took 1 ml of splenocyte suspension (2 x 10 6 cells/ml) in each test tubes marked as test and a control. Added 0.1 ml NBT in tube marked as test containing different number of cells as 1 x 10 6 cells ml -1 and 1 x 10 9 cells ml -1 for each strain. Incubated both the tubes for 20 minutes at 37 C, centrifuged it and washed the pellets with PBS buffer. Then, added 0.1 N H Cl to stop the reaction, again centrifuged for 10 minutes at 400 g and removed the supernatant and added 5 ml dioxan to both test and control marked tubes. Incubated the tubes for 20 minutes at 70 C and then centrifuged, noted the absorbance of supernatant at 520 nm, using dioxan as blank.
3 % NBT reduction : O.D. of test (T) O.D. of control (C) 100 O.D. of control (C) Inducible Nitric Oxide Synthase activity The inducible nitric oxide Synthase activity was assessed as described by Stuehr and Marletta (1987) based upon the principle that when macrophages are activated they together with T cell derived IFN-gamma begin to express inos which oxidizes L-Arginine to citrulline and nitric oxide. The dark purplish colour so formed is extractable with griess reagent which can be measured spectro-photometrically at 450 nm using griess reagent as blank. 1. L-Arginine:0.2M in normal saline (0.85 % NaCl) 2. Griess reagent: (a) NEDD (Napthyl ethylene dianiline dihydro chloride): Dissolved 0.1g of NEDD in 100 ml sterile distilled water. (b) Sulphanilamide: Dissolved 1 % solution of sulphanilamide (1g in 100 ml distilled water) in 100 ml in 2.5 % orthophospheric acid The Griess reagent was prepared by mixing above solution (a) and (b) in ratio 1:1. Inducible nitric oxide Synthase activity was assessed using Griess reagent. Briefly, splenocytes were incubate with different number of cells as 1 x 10 6 cells ml -1 and 1 x 10 9 cells ml -1 for each strain at 37 C for 24 hour in humidified 5 % CO 2 atmosphere. To the adherent cells mixture added freshly prepared 1 ml MEM and 20 µl of L-arginine solution (0.2M), incubated at 37 C for 24 hour in humidified 5 % CO 2 atmosphere, centrifuged and collected the supernatant.
4 The supernatant was treated with freshly prepared Griess reagent and kept in dark for 10 min. Absorbance was measured spectro-photometrically at 540nm against control. Bactericidal activity (Raghuramulu et al., 1983) of splenocyte. Bactericidal activity is one of the important parameter to measure the Phagocytic function 1. Nutrient medium: 10g Beef extract, 10g peptone and 1 g of NaCl dissolved in distilled water and volume made one litre. 2. Nutrient Agar: 1g Beef extract, 2g yeast extract, 1g NaCl, 1g peptone and 15g agar dissolved in distilled water and final volume made to 1 litre. 3. Kreb s Ringer Phosphate (KRP) buffer: 100 parts of 0.9% NaCl, 4 parts of 1.15 % KCl, 3 parts of 1.22% CaCl 2, 1 part of 3.82 % Mg SO 4.7H 2 O and 20 parts of phosphate buffer dissolved in distilled water. Phosphate buffer was prepared by dissolving 17.8g Na 2 HPO 4 and 20 ml of 1N HCl in 1 litre distilled water. The ph of KRP buffer was 7.4 without further adjustment. E.coli grown in nutrient broth for h at 37 C was collected and centrifuged. The pelleted bacteria was washed thrice in KRP buffer and finally suspended in saline and standardized the number of E.coli ( ) by measuring absorbance at 600 nm. Took 3ml of suspension of splenocytes under sterile conditions, centrifuged at 800 g for 5 min and washed the cells with buffer. Took two test tubes, in one test tube (marked test) added 500 µl of splenocytes /ml and 500 µl of different number of cells as 1 x 10 6 cells ml -1 and 1 x 10 9 cells ml -1 for each strain and 80 µl of E.coli ( cells/ml) and in other test tube (marked control) added only 80 µl E.coli ( cells/ml). Volume was made 2 ml by adding KRP buffer. After incubation at 37 C for 1
5 hour, took 0.2ml of reaction mixture from test tube and added 0.8 ml of distilled water to lyse the splenocytes. Diluted each sample 10 8 times with normal saline, counted number of viable bacteria by plating diluted sample onto agar plates after 24 h at 37 C. Calculated the percent phagocytosis with the following formula: % Bactericidal Activity: CFU/ml in control CFU/ml in test 100 CFU/ml in control Direct Haemagglutination (Haghighi et al., 2005) Reagents 1. Phosphate Buffer Saline 2. Sheep Red Blood Cells Briefly serum sample were incubated at 56 C for 30 min to inactivate the complement. 50µL of PBS containing 0.05% BSA was dispensed into each well of round bottomed 96- well microplate. Serum samples (50 µl) were then added and serially double diluted in the wells from columns 2 to 12. The first column (PBS only) of wells was considered as blank. Then, 50 µl of 1% SRBC in PBS was added to all the wells to make a 100 µl final volume. Subsequently, the plates were shaken for 1 min and incubated for 24 hrs at 37 C and agglutination was monitored visually. Highest dilution capable of visible agglutination was considered as the antibody titer. The results were expressed as mean ± S.E.M log 2 titer o individual animal. Cell mediated immune response Delayed Type Hypersensitivity assay (Titus and Chiller, 1981) Reagents
6 1. Normal saline (0.84% NaCl) 2. Sheep Red Blood Cells All SRBC primed groups were challenged intradermally on day 15 th with SRBC suspension ( /100µl saline) in the hind footpad. The control lateral paw was given equal volume of saline. Paw thickness was measured with micro-caliper at 24h interval up to 72h. The difference in paw thickness compared to control was taken as a measure of DTH and expressed in millimeter. Results are expressed as mean ± S.E.M. of footpad thickness up to 72h expressed in millimeter. Detection of anti BSA antibodies by Indirect ELISA (Haghighi et al., 2005) 1. PBST (PBS with 0.05% Tween 20) 2. Blocking buffer (PBST containing 0.25% gelatin) 3. p-nitrophenylphosphate solution For evaluation of serum antibodies, each well of a flat-bottomed 96-well microplate was coated overnight with 100 μl coating buffer (ph 9.6) containing BSA (30 μg/ml) at 4 C. The wells were washed three times with 200 μl PBST with complete decanting between each wash. Subsequently, 100 μl of blocking buffer was added to each well and incubated for 1 h at 37 C to occupy all unbound sites. Washing was repeated as described above, followed by the addition of 100 μl mouse serum, diluted 1:100 in blocking buffer, to each well. Plates were incubated for 2 h at room temperature and then washed three times, and 100 μl of goat anti-mouse IgG-Fc or goat anti-mouse IgM conjugated with alkaline phosphatase (diluted 1:500 in blocking buffer) was added to each
7 well and incubated for 1 h at room temperature (RT) before the plate was washed three times. 100µL p-nitrophenylphosphate solution was added as the substrate to each well and incubated for 1 h at room temperature in the dark. The absorbance was measured at 405 nm using a microplate reader. Cytokine estimation to study the expression of IL-4 and IFN-gamma by Enzyme Linked Immunosorbent Assay (ELISA) Provided by the manufacturer Cytokine estimation was performed in cell culture suspension as per the instructions of the manufacturer (BD OptEIA set) by ELISA. Briefly, 96-well microtiter ELISA plate were coated overnight with 50µL of capture antibodies in carbonate bicarbonate buffer, ph 9.6 at 4 C. After washing, the wells were incubated with 200µL of blocking buffer for 1 h at room temperature and followed by addition of 100 µl serum samples. The respective cytokine as standard were used in triplicates. Plates were kept at room temperature for 2h, washed five times with wash buffer.biotin labeled secondary antibody was added along with avidin horse radish peroxidase (AV-HRP). The wells were washed and substrate, tetramethylbenzidine and hydrogen peroxide were added to each well and incubated at room temperature for the development of color. The reaction was stopped with 1M H3 PO4 and plates were read at 450 nm. The cytokines were quantified using recommended cytokines standards (BD Opt EIA set).
SensoLyte pnpp Alkaline Phosphatase Assay Kit *Colorimetric*
SensoLyte pnpp Alkaline Phosphatase Assay Kit *Colorimetric* Catalog # 72146 Kit Size 500 Assays (96-well plate) Optimized Performance: This kit is optimized to detect alkaline phosphatase activity Enhanced
More informationHuman Apolipoprotein A1 EIA Kit
A helping hand for your research Product Manual Human Apolipoprotein A1 EIA Kit Catalog Number: 83901 96 assays 1 Table of Content Product Description 3 Assay Principle 3 Kit Components 3 Storage 4 Reagent
More information2-Deoxyglucose Assay Kit (Colorimetric)
2-Deoxyglucose Assay Kit (Colorimetric) Catalog Number KA3753 100 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
More informationTECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C
Phospho-Stat3 (ptyr 705 ) and pan-stat3 ELISA Kit for detection of human, mouse, or rat phospho-stat3 (ptyr 705 ) and pan-stat3 in cell and tissue lysates Catalog Number RAB0447 Storage Temperature 20
More informationProthrombin (Human) ELISA Kit
Prothrombin (Human) ELISA Kit Catalog Number KA0496 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General
More informationSTAT1 (ps727) (Human/Mouse) ELISA Kit
STAT1 (ps727) (Human/Mouse) ELISA Kit Catalog Number KA2171 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION STAT1 (ps727) (Human/Mouse) ELISA (Enzyme-Linked Immunosorbent
More informationSTAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit
STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit Catalog Number KA2176 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Principle of the Assay...
More informationTECHNICAL BULLETIN. Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates
Phospho-Akt (pser 473 ) ELISA Kit for detection of human, mouse, or rat phospho-akt (pser 473 ) in cell and tissue lysates Catalog Number RAB0011 Storage Temperature 20 C TECHNICAL BULLETIN Product Description
More informationHuman TSH ELISA Kit. User Manual
Human TSH ELISA Kit User Manual Catalog number: GTX15585 GeneTex Table of Contents A. Product Description... 2 B. Kit Components... 3 C. Additional Required Materials (not included)... 3 D. Reagent Preparation...
More informationRayBio Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 ELISA Kit
RayBio Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 ELISA Kit Catalog #: PEL-NFKBP65-S536-T User Manual Last revised October 10, 2017 Caution: Extraordinarily useful information
More informationRayBio Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit
RayBio Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit Catalog #: PEL-DDR2-Y740-T User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607
More informationEGFR (py1045)/ Pan EGFR (Human) ELISA Kit
EGFR (py1045)/ Pan EGFR (Human) ELISA Kit Catalog Number KA2156 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION EGFR (py1045)/pan EGFR (Human) ELISA (Enzyme-Linked Immunosorbent
More informationRayBio Human Phospho-DDR1 (Tyr792) ELISA Kit
RayBio Human Phospho-DDR1 (Tyr792) ELISA Kit Catalog #: PEL-DDR1-Y792 User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane,
More informationSTAT3 (py705) (Human/Mouse/Rat) ELISA Kit
STAT3 (py705) (Human/Mouse/Rat) ELISA Kit Catalog Number KA2175 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION STAT3 (py705) (Human/Mouse/Rat) ELISA (Enzyme-Linked
More informationRayBio Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit
RayBio Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit Catalog #: PEL-DDR1-Y792-T User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607
More informationRayBio Human Phosphotyrosine BTK ELISA Kit
RayBio Human Phosphotyrosine BTK ELISA Kit Catalog #: PEL-BTK-Y User Manual Last revised August 10, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
More informationAac Reagent Set ELISA for the detection of Acidovorax avenae subsp. citrulli Catalog number: SRA 14800
List of contents Lot number Aac Reagent Set Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Alkaline phosphatase enzyme conjugate 0.150 ml 0.275 ml 0.525
More informationEnzyme Immunoassay for
Enzyme Immunoassay for Prostaglandin E 2 For Research Use Only INTRODUCTION Prostaglandin E 2 EIA Kit Product Number: EA02 Store at 4 C FOR RESEARCH USE ONLY Document Control Number: EA02.120214 Page 1
More informationhuman Total Cathepsin B Catalog Number: DY2176
human Total Cathepsin B Catalog Number: DY2176 This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Total
More informationInfluenza A IgG ELISA
Influenza A IgG ELISA For the qualitative determination of IgG-class antibodies against Influenza A virus in human serum or plasma (citrate, heparin). For Research Use Only. Not For Use In Diagnostic Procedures.
More informationHuman LDL ELISA Kit. Innovative Research, Inc.
Human LDL ELISA Kit Catalog No: IRKTAH2582 Lot No: SAMPLE INTRODUCTION Human low-density lipoprotein (LDL) transports cholesterol from the liver to tissues where it is incorporated into cell membranes.
More informationRat cholesterol ELISA Kit
Rat cholesterol ELISA Kit Catalog No. CSB-E11706r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat Cholesterol concentrations in serum, plasma and other biological fluids.
More informationGlobal Histone H3 Acetylation Assay Kit
Global Histone H3 Acetylation Assay Kit Catalog Number KA0633 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationHuman HBcAb IgM ELISA kit
Human HBcAb IgM ELISA kit Catalog number: NR-R10163 (96 wells) The kit is designed to qualitatively detect HBcAb IgM in human serum or plasma. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PURPOSES
More informationMTS assay in A549 cells
Project: VIGO MTS assay in A549 cells Detection of cell viability/activity AUTHORED BY: DATE: Cordula Hirsch 20.01.2014 REVIEWED BY: DATE: Harald Krug 10.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective
More information20X Buffer (Tube1) 96-well microplate (12 strips) 1
PROTOCOL MitoProfile Rapid Microplate Assay Kit for PDH Activity and Quantity (Combines Kit MSP18 & MSP19) 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 MSP20 Rev.1 DESCRIPTION MitoProfile Rapid Microplate
More informationReagent Set DAS ELISA, Alkaline phosphatase label SRA 22001, SRA 23203, SRA 27703, SRA & SRA ToRSV, ArMV, GFLV, AnFBV and PDV
List of contents Lot number Reagent Set Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Alkaline phosphatase enzyme conjugate 0.150 ml 0.275 ml 0.525
More informationInfluenza A H1N1 HA ELISA Pair Set
Influenza A H1N1 HA ELISA Pair Set for H1N1 ( A/Puerto Rico/8/1934 ) HA Catalog Number : SEK11684 To achieve the best assay results, this manual must be read carefully before using this product and the
More informationEliKine Free Thyroxine (ft4) ELISA Kit
EliKine Free Thyroxine (ft4) ELISA Kit Booklet Item NO. KET0005 Product Name EliKine Free Thyroxine (ft4) ELISA Kit ATTENTION For laboratory research use only. Not for clinical or diagnostic use TABLE
More informationRayBio Human Granzyme B ELISA Kit
RayBio Human Granzyme B ELISA Kit Catalog #: ELH-GZMB User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationNF-κB p65 (Phospho-Thr254)
Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 NF-κB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02015 Please read the provided manual
More informationEPIGENTEK. EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric) Base Catalog # P-4059 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric) Base Catalog # P-4059 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric)
More informationRayBio Human ENA-78 ELISA Kit
RayBio Human ENA-78 ELISA Kit Catalog #: ELH-ENA78 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationEPIGENTEK. EpiQuik Global Histone H3 Acetylation Assay Kit. Base Catalog # P-4008 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Global Histone H3 Acetylation Assay Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global
More informationCoQ10(Coenzyme Q10) ELISA Kit
CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:
More informationTotal Histone H3 Acetylation Detection Fast Kit (Colorimetric)
Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) Catalog Number KA1538 48 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use...
More informationCaspase-3 Assay Cat. No. 8228, 100 tests. Introduction
Introduction Caspase-3 Assay Cat. No. 8228, 100 tests Caspase-3 is a member of caspases that plays a key role in mediating apoptosis, or programmed cell death. Upon activation, it cleaves a variety of
More informationEPIGENTEK. EpiQuik Global Histone H4 Acetylation Assay Kit. Base Catalog # P-4009 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Global Histone H4 Acetylation Assay Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Global Histone H4 Acetylation Assay Kit is suitable for specifically measuring global
More informationHuman Aldosterone ELISA Kit
Human Aldosterone ELISA Kit Cat. No.:DEIA2002 Pkg.Size:96T Intended use For determination of Aldosterone by enzyme immunoassay in human serum by enzyme immunoassay. Hydrolysis is necessary for the determination
More informationMouse Cathepsin B ELISA Kit
GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Phone: 858.458.0866 Fax: 858.458.0833 Email: techline@genwaybio.com http://www.genwaybio.com Mouse Cathepsin B ELISA Kit Catalog No. GWB-ZZD154
More informationHuman Cathepsin D ELISA Kit
GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Phone: 858.458.0866 Fax: 858.458.0833 Email: techline@genwaybio.com http://www.genwaybio.com Human Cathepsin D ELISA Kit Catalog No. GWB-J4JVV9
More informationRayBio Human Thyroglobulin ELISA Kit
RayBio Human Thyroglobulin ELISA Kit Catalog #: ELH-Thyroglobulin User Manual Last revised July 6, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
More informationEpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)
EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)
More informationEXOTESTTM. ELISA assay for exosome capture, quantification and characterization from cell culture supernatants and biological fluids
DATA SHEET EXOTESTTM ELISA assay for exosome capture, quantification and characterization from cell culture supernatants and biological fluids INTRODUCTION Exosomes are small endosome-derived lipid nanoparticles
More informationAMPK Phosphorylation Assay Kit
AMPK Phosphorylation Assay Kit Catalog Number KA3789 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationHuman LDL Receptor / LDLR ELISA Pair Set
Human LDL Receptor / LDLR ELISA Pair Set Catalog Number : SEK10231 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in
More informationMTS assay in THP-1 cells
Project: VIGO MTS assay in THP-1 cells Detection of cell viability/activity AUTHORED BY: DATE: Cordula Hirsch 20.01.2014 REVIEWED BY: DATE: Harald Krug 10.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective
More informationHuman Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit
Human Thyroid-Peroxidase Antibody, TPO-Ab ELISA Kit Catalog No: E0442h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH
More informationACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man Coulter HIV-1 p24 ELISA May 21, 2004
Coulter HIV p24 1. PRINCIPLE The Human Immunodeficiency Virus Type 1 (HIV-1) is recognized as the etiologic agent of acquired immunodeficiency syndrome (AIDS). The virus is transmitted by sexual contact,
More informationInfluenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set
Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin / HA ELISA Pair Set Catalog Number : SEK40103 To achieve the best assay results, this manual must be read carefully before using this product and the assay
More informationSIV p27 Antigen ELISA Catalog Number:
INTENDED USE The RETRO-TEK SIV p27 Antigen ELISA is for research use only and is not intended for in vitro diagnostic use. The RETRO-TEK SIV p27 Antigen ELISA is an enzyme linked immunoassay used to detect
More informationMouse Leptin ELISA Kit (mleptin-elisa)
Mouse Leptin ELISA Kit (mleptin-elisa) Cat. No. EK0438 96 Tests in 8 x 12 divisible strips Background Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the
More informationSupplementary Information. Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food
Supplementary Information Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative Lipsy Chopra, Gurdeep Singh, Kautilya Kumar Jena and Debendra K. Sahoo* Biochemical
More informationHuman HIV (1+2) antigen&antibody ELISA Kit
Human HIV (1+2) antigen&antibody ELISA Kit Catalog Number. CSB-E18042h For the qualitative determination of human HIV (1+2) antibody and P24 antigen concentrations in serum, plasma. This package insert
More informationMouse Myeloperoxidase/MPO ELISA Kit
OriGene Technologies, Inc 9620 Medical Center Dr., Suite 200, Rockville, MD 20850 Phone: 1.888.267.4436 Fax: 301-340-9254 Email: techsupport@origene.com Web: Mouse Myeloperoxidase/MPO ELISA Kit Catalog
More informationHepatitis A virus IgM ELISA Kit
Hepatitis A virus IgM ELISA Kit Catalog Number KA0285 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationReagent Set DAS ELISA, Alkaline phosphatase label
List of contents Lot number Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Alkaline phosphatase enzyme conjugate 0.150 ml 0.275 ml 0.525 ml 2.525 ml
More informationInfluenza B Hemagglutinin / HA ELISA Pair Set
Influenza B Hemagglutinin / HA ELISA Pair Set Catalog Number : SEK11053 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized
More informationHosta virus X ELISA KIT
Hosta virus X ELISA KIT Cat. No.:DEIA9256 Pkg.Size:96T Intended use The Hosta virus X ELISA KIT is used to detect HVX in both leaves and roots of hosta plants. Reagents And Materials Provided 1. Capture
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More informationHuman Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set
Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set Catalog Number : SEK11695 To achieve the best assay results, this manual must be read carefully before using this product
More informationTo detect antibodies to Avian Influenza (AI) using the haemagglutination inhibition test in avian serum specimens 2.
SADC Harmonized SOP for Avian Influenza HA and HI Serological Tests Prepared by: Dr. P.V. Makaya, Dr. Joule Kangumba and Ms Delille Wessels Reviewed by Dr. P.V. Makaya 1. Purpose and scope To detect antibodies
More informationMouse C3 (Complement Factor 3) ELISA Kit
Mouse C3 (Complement Factor 3) ELISA Kit Cat. No.:DEIA8289 Pkg.Size:96T Intended use The Mouse C3 (Complement Factor 3) ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring
More informationMouse TrkB ELISA Kit
Mouse TrkB ELISA Kit CATALOG NO: IRKTAH5472 LOT NO: SAMPLE INTENDED USE For quantitative detection of mouse TrkB in cell culture supernates, cell lysates and tissue homogenates. BACKGROUND TrkB receptor
More informationProtease Assay. (Cat. # ) think proteins! think G-Biosciences
389PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Protease Assay (Cat. # 786 028) think proteins! think G-Biosciences www.gbiosciences.com
More informationThyroid Stimulating Hormone (S-TSH) Thyroid Stimulating
ab108659 Thyroid Stimulating Hormone (S-TSH) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thyroid Stimulating Hormone (S-TSH) concentrations in serum. This product is
More informationAFLATOXIN M1 CAT. NO. 961AFLMO1M
h AFLATOXIN M1 CAT. NO. 961AFLMO1M Competitive ELISA Immunoassay for the quantitative detection of Aflatoxin M1 in milk, milk powder and cheese. General Aflatoxins are toxic metabolites produced by a variety
More informationPh. Eur. Reference Standard - LEAFLET
European Directorate for the Quality of Medicines & HealthCare European Pharmacopoeia (Ph. Eur.) 7, Allée Kastner CS 30026, F-67081 Strasbourg (France) Tel. +33 (0)3 88 41 20 35 Fax. + 33 (0)3 88 41 27
More informationLH (Rodent) ELISA Kit
LH (Rodent) ELISA Kit Catalog Number KA2332 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationBarley yellow dwarf virus Reagent Set Compound ELISA, Alkaline phosphatase label Catalog number: SRA (BYDV-MAV), SRA (BYDV-PAV)
List of contents Lot number Barley yellow dwarf virus Reagent Set Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Detection Antibody, bottle A 0.150 ml
More informationGlucose Uptake Colorimetric Assay Kit
ab136955 Glucose Uptake Colorimetric Assay Kit Instructions for Use For the sensitive and accurate measurement of Glucose uptake in various samples This product is for research use only and is not intended
More informationHBeAg and HBeAg Ab ELISA Kit
HBeAg and HBeAg Ab ELISA Kit Catalog Number KA0290 96 assays Version: 17 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3
More informationPotato leafroll virus (PLRV) Reagent Set DAS ELISA, Alkaline phosphatase label Catalog number: SRA List of contents
Potato leafroll virus (PLRV) Reagent Set List of contents Lot number Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Alkaline phosphatase enzyme conjugate
More informationCanine Thyroid Stimulating Hormone, TSH ELISA Kit
Canine Thyroid Stimulating Hormone, TSH ELISA Kit Catalog No: E0463c 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH
More informationHuman Alpha 1 microglobulin ELISA Kit
Human Alpha 1 microglobulin ELISA Kit Catalogue No.: EH4144 Size: 48T/96T Reactivity: Human Range:0.625-40ng/ml Sensitivity:
More informationAssayMax Human Aldose Reductase ELISA Kit
AssayMax Human Aldose Reductase ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing
More informationHIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual)
HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual) BACKGROUND Human Immunodeficiency Virus ( HIV ) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to
More informationBlueberry scorch virus Reagent Set DAS ELISA, Alkaline phosphatase label for BlScV Catalog number: SRA 19100
List of contents Lot number Blueberry scorch virus Reagent Set Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Alkaline phosphatase enzyme conjugate 0.150
More information02006B 1 vial 02006B 1 vial Store at -20 C. Lyophilized recombinant IL-2
For detection and measurement of human interleukin 2 Catalog #02006 Catalog #02007 2 Plates 10 Plates Product Description The Human Interleukin 2 (IL-2) ELISA Kit is designed for the quantitative detection
More informationApolipoprotein A-1 ELISA
Apolipoprotein A-1 ELISA For the quantitative determination of apolipoprotein A1 in serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical
More informationab Human Citrate Synthase (CS) Activity Assay Kit
ab119692 Human Citrate Synthase (CS) Activity Assay Kit Instructions for Use For the measurement of mitochondrial citrate synthase (CS) activity in Human samples This product is for research use only and
More informationSupplementary Files S1 Isolation of Monocytes S2 Haemolysis study Reagents Procedure S3 Cytotoxicity studies Trypan blue dye exclusion method
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Supplementary Files S1 Isolation of Monocytes A 3 ml volume of Histopaque 1083 solution was
More informationLH (Bovine) ELISA Kit
LH (Bovine) ELISA Kit Catalog Number KA2280 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationGlutathione Assay Kit
Glutathione Assay Kit Catalog Number KA1649 250 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationHuman Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set
Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Catalog Number : SEK11233 To achieve the best assay results, this manual must be read carefully before using this product
More informationMitochondrial Trifunctional Protein (TFP) Protein Quantity Microplate Assay Kit
PROTOCOL Mitochondrial Trifunctional Protein (TFP) Protein Quantity Microplate Assay Kit DESCRIPTION Mitochondrial Trifunctional Protein (TFP) Protein Quantity Microplate Assay Kit Sufficient materials
More informationSerum Amyloid A ELISA
Serum Amyloid A ELISA For the quantitative determination of serum amyloid A (SAA) in serum plasma For Research use Only. Not for Use in Diagnostic Procedures Please see Appendix A for Reference Serum information
More informationFSH (Rodent) ELISA Kit
FSH (Rodent) ELISA Kit Catalog Number KA2330 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationOxiSelect MDA Adduct ELISA Kit
Revised Protocol Product Manual OxiSelect MDA Adduct ELISA Kit Catalog Number STA-332 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Lipid peroxidation is a well-defined
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More informationT3 (Total) (Human) ELISA Kit
T3 (Total) (Human) ELISA Kit Catalog Number KA0198 96 assays Version:02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of
More informationab Glucose Uptake Assay Kit (colorimetric) 1
Version 16 Last updated 10 January 2018 ab136955 Glucose Uptake Assay Kit (Colorimetric) For the measurement of Glucose uptake in a variety of cells. This product is for research use only and is not intended
More informationPVY Reagent Set Compound ELISA, Alkaline phosphatase label Potato virus Y Catalog number: SRA 20001
List of contents Lot number PVY Reagent Set Item 96 wells 500 wells 1000 wells 5000 wells Capture antibody 0.150 ml 0.275 ml 0.525 ml 2.525 ml Detection antibody, bottle A 0.150 ml 0.275 ml 0.525 ml 2.525
More informationCereal yellow dwarf virus - RPV ELISA Kit
Cereal yellow dwarf virus - RPV ELISA Kit Cat. No.:DEIA9232 Pkg.Size:96T Intended use The Cereal yellow dwarf virus - RPV ELISA Kit is a qualitative serological assay for the detection of CYDV-RPV in plant
More informationWHO Prequalification of Diagnostics Programme PUBLIC REPORT. Product: Bioelisa HCV 4.0 Number: PQDx Abstract
WHO Prequalification of Diagnostics Programme PUBLIC REPORT Product: Bioelisa HCV 4.0 Number: PQDx 0165-060-00 Abstract Bioelisa HCV 4.0 with product codes 3000-1115 and 3000-1116, manufactured by Biokit
More informationHIV-1 p24 Antigen ELISA Catalog Number:
INTENDED USE The RETRO-TEK HIV-1 p24 Antigen ELISA is supplied for research purposes only. It is not intended for use in the diagnosis or prognosis of disease, or for screening and may not be used as a
More information