Presentation Outline Ojectives Results Conclusions

Transcription

1 Effects of LEDs on Photosynthesis and Secondary Metaolites in Roses, Chrysanthemums, and Campanulas Theoharis Ouzounis 1, Xavier Fretté 1, Eva Rosenqvist 2, Carl Otto Ottosen 3 1 Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark, Odense, Denmark 2 Department of Agriculture and Ecology, Crop Science, University of Copenhagen, Taastrup, Denmark 3 Department of Food Science, Aarhus University, Aarslev, Denmark

2 Presentation Outline Ojectives Results Conclusions

3 Ojectives To characterize the effect of LED lighting in horticultural ornamentals grown in greenhouse facilities on the photosynthetic characteristics and the physiological mechanisms of selected plant species on secondary metaolites which play a crucial role in the iochemical defense of the plant

4 Roses (Rosa hyrida Scarlet ) Plant material Chrysanthemums (Chrysanthemum morifolium Coral Charm ) Campanulas (Campanula portenschlagiana BluOne ) The plants were grown to flowering (except chrysanthemums) and plant growth was recorded at the end of the experiment

5 Greenhouse Setup LED array yielding approximately 200 µmol m-2s-1 for 16 hours per day t p e c The temperature in the greenhouse compartments was set to 24 oc and 18 oc during the day and night, respectively n o C (1) 40% Blue 60% Red (2) 20% Blue 80% Red (3) 100% Red (4) 100% White or Control (C)

6 Photosynthetic measurements Open gas exchange systems CIRAS-2. Real-time measurements of CO 2 uptake, transpiration, stomatal conductance (g s ), and intercellular CO 2 Fresh and dry weight of the stems and leaves, leaf area, and plant height

7 Chemical analysis Leaf samples are taken randomly for later analysis y HPLC/LCMS Samples were ground with liquid nitrogen and 80% MeOH was used for extraction Separations with a Zorax Eclipse XDB-C18 column (5μm, 150 X 4.6 mm; Agilent) Phenolic acids and flavonoids will e quantified in extracts y HPLC and LCMS at 320 nm and 360 nm, respectively

8 Growth parameters ROSES Total FW and DW: Highest percentage in the 100%R (up to 15% increase compared the Control) Leaf area: 40%B/60%R, 20%B/80%R, and 100%R treatments (up to 30% higher than the Control) Plant height: Not affected Numer of color uds: Not affected Pure RED demonstrated morphological anormalities

9 Growth parameters CHRYSANTHEMUMS Total FW, DW, and Leaf Area: Higher for oth BLUE/RED treatments with NO significant difference among treatments Plant height: Not really affected CAMPANULAS Total FW and DW: NO difference among treatments Leaf Area: Higher for 20%B/80%R and the Control with NO significant difference etween them Plant height: Not affected

10 Net photosynthesis and stomatal conductance Pn (mmol m -2 s -1 ) P n : NO effect among treatments g s : Higher for 40%B/60%R and 20%B/80%R % B / 60% R 20% B / 80% R 100%R Control Chrysanthemums gs (mmol m -2 s -1 ) % B / 60% R 20% B / 80% R 100%R Control *Same for all 3 plants *Same for all 3 plants Chrysanthemums PAR ( mol m -2 s -1 ) PAR ( mol m -2 s -1 )

11 Roses HPLC and LCMS 40%B/60%R: Highest amount of phenolic acids and flavonoids Same for neochlorogenic acid, quercetin, and others PeakArea PeakArea a a 40%B / 60%R 20%B / 80%R 100%R C a Treatments 64% Roses - Chlorogenic acid 82% 81% Roses - Rutin 68% 76% 75% 0 40%B / 60%R 20%B / 80%R 100%R C Treatments

12 Chrysanthemum HPLC and LCMS 40%B/60%R and 20%B/80%R: Highest amount of flavonoids NO Phenolics detected Same for kaempferol glucoside, apigenin glucuronide, and others PeakArea PeakArea a Chrysanthemums - Apigenin derivative a 40%B / 60%R 20%B / 80%R 100%R C a 22% a Treatments Chrysanthemums - Acacetin a 19% 48% 24% 64% 66% %B / 60%R 20%B / 80%R 100%R C Treatments

13 Campanulas HPLC and LCMS 40%B/60%R and 20%B/80%R: Highest amount of phenolic acids and flavonoids Same for neochlorogenic acid, rutin, and others PeakArea PeakArea a Campanulas - Flavonoid unknown %B / 60%R 20%B / 80%R 100%R C Treatments Campanulas - Chlorogenic acid a a 15% a 41% 66% 54% 53% 3% %B / 60%R 20%B / 80%R 100%R C Treatments

14 Conclusions The comination of RED and BLUE LED lighting has a positive effect on growth and development of roses, chrysanthemum and campanulas Pure RED light increases total fresh and dry weight BUT demonstrates the most extensive anormalities BLUE/RED lighting treatment seem to have no significant effect on photosynthesis Stomatal conductance increases with increasing amount of BLUE light The amount of secondary metaolites increases with additional of BLUE light

15 Acknowledgements Dr Xavier Fretté University of Southern Denmark Dr Carl Otto Ottosen University of Aarhus Dr Eva Rosenqvist University of Copenhagen University of Southern Denmark Fionia Lighting EUDP GreenGrowing ERDF Project

16 Ευχαριστώ για την προσοχή σας Tak for jeres opmærksomhed Thank you for your attention