chapter 17: specific/adaptable defenses of the host: the immune response

Transcription

1 chapter 17: specific/adaptable defenses of the host: the immune response

2 defense against infection & illness body defenses innate/ non-specific adaptable/ specific epithelium, fever, inflammation, complement, interferon, macro/dcs immunity lymphoid cells humoral cell-mediated non-self Ag Ab & B cells altered self Ag APCs & T cells

3 lymphoid cells: B cells & T cells naïve lymphocytes highly specific (Ag) >10 6 at a time billions made/day live weeks to months

4 adaptable cells bind Antigen (Ag), not PAMP Ag: bound by WBC receptors BCR TCR Ig/Ab

5 getting info to lymphocytes naïve B cells and T cells live in lymph nodes, Ag comes to them

6 lymphoid cell activation: B cells & T cells Ag travels to lymph node alone (B) or attached to APC (B/T) Ag encountered by lymphocyte lymphocyte is activated lymphocyte clonally expands activated T cells bloodstream B cell Ab bloodstream extravasation at infection site **extravasation video takes about 4 seconds to load**

7 humoral immunity T cell independent response: weak, short, affinity activation takes several h plasma cells w/in 1 wk memory cells w/in 2 wks

8 antibodies (Ab) memory acute phase

9 results of Ag-Ab binding

10 Ab-dependent cell-mediated cytotoxicity (ADCC) Ab-bound target cell lysed by natural killer (NK) cells & eosinophils large parasites are too big for phagocytosis & integument is resistant 1. IgE coats parasites 2. Fc receptor of eosinophil recognizes IgE 3. helminth-bound IgE causes the eosinophil to degranulate (release cytotoxins from granules) NK cells activated by antibodies 1. NK Fc receptor recognizes the Fc portion of Ab bound to target cell 2. NK cell releases cytokines and cytotoxic granules 3. these enter the target cell and trigger apoptosis

11 chapter 17 learning objectives 1. What kinds of pathogens do the humoral and cell-mediated branches of the immune system target? How do the terms self, non-self and altered-self relate to this discussion? 2. What are the differences between T cells and B cells, in terms of place of maturation and receptors? 3. What is the lymph system? What is the difference between lymph, interstitial fluid and blood? 4. Where are MHC class I, MHC class II, BCRs and TCRs found? 5. In class, we discussed three ways that B cells can be activated and clonally selected. Understand how each of these results in the production of both plasma cells and memory cells. 6. Draw and label the structure of an antibody molecule. Include the following components: light chain, heavy chain, variable region, constant region, disulfide bonds, antigen binding site, and antigen. Compare/contrast IgG and IgM. 7. What five things result from the binding of Ab to Ag? How do the two types of Ab-dependent Cell-mediated cytotoxicity discussed in class differ?

12 cell-mediated immunity: T cells & APCs Major HistoCompatibility molecules present Ag to TCR MHC Class I on all body cells, MHC Class II only APCs

13 MHC class I molecules & Cytotoxic T (T c ) cells perforin production granzyme production

14 MHC class II molecules & Helper T (T H ) cells

15 T H cell activation of innate cells

16 T H cell dependent B cell activation

17 T H activation of CD8 T C cells

18 T cell activation & memory

19 the helper T cell

20 Pathogen group lymphocyte response adaptive response Ab (B cell) T C cells (CTLs) T H cells extracellular bacteria bound Ab initiates total immune response* not effective B cell expansion no direct role in elimination intracellular vesicular bacteria internal pathogen renders Ab useless cytosolic replication of bacteria can cause T C response hyperactivation of macrophages* viruses free viruses are bound & neutralized T C cells kill virallyinfected cells* activate B cell vs. free viruses & NK cells vs. infected cells no direct role in elimination helminths antibody-mediated cell-dependent cytotoxicity (ADCC)* not effective B cell expansion no direct role in elimination enhance ADCC

21 comparing the innate & adaptive responses Innate Adaptive phagocytes, NK cells, eosinophils, mast cells response present from birth T cells and B cells response develops in first year of life responds to infection within minutes to hours responds to infection after a few days PRRs on phagocytes recognize common pathogen structural patterns BCR & TCRs recognize specific epitope structures all cells of one type have the same receptors each B cell or T cell has a different Ag specificity not clonal react to common pathogen ligands defense in any connective tissue in body no memory generated clonal response by Ag-specific effector T & plasma B cells defense generated in 2 o lymphoid tissue generates memory

22 coordinated innate & immune attack

23 coordinated humoral & cell-mediated attack

24 chapter 17 learning objectives 1. What kind of cells produce MHC class I molecules? Which produce MHC class II? Which T cells interact with these and what does the interaction between T cell and MHC molecule cause? 2. Compare and contrast the Helper T cell and the Cytotoxic T cell (cytotoxic T lymphocyte) 3. How is memory produced in the cell mediated branch of the immune system? 4. Distinguish between the antibody-mediated (humoral) immune system and T cell-mediated immune system. Know the steps and key players in both. 5. Which is the best lymphocyte response for: extracellular bacteria, intracellular vesicular bacteria, viruses and helminths? 6. Compare and contrast innate and adaptable defenses for: response time, specificity, memory, & location of defense. 7. As discussed in class, identify the role of each of the following: lymphoid cell, B cell, T cell, NK cell, eosinophil, dendritic cell, monocyte, and macrophage.