RealLine HCV Quantitative - Uni-Format
|
|
- Malcolm Sharp
- 6 years ago
- Views:
Transcription
1 Instructions for use - KIT FOR EXTRACTION OF RNA AND SUBSEQUENT REAL TIME PCR DETECTION AND QUANTIFICATION OF HEPATITIS C VIRUS RNA Attention! Please read the information about quantification process carefully! Research Use Only (RUO) RealLine HCV quantitative Uni Format incl. extraction VBD Tests valid from: April 2017 Rev _EN Page 1 of 16
2 Explanation of symbols used in labeling IVD LOT REF For in vitro diagnostic use Batch code Catalogue number Amount of tests Expiry Date Temperature limitation Consult instructions for use Keep out of sunlight Manufacturer BIORON Diagnostics GmbH Rheinhorststr Ludwigshafen (Germany) Phone Fax: Legals: Limited Product Warranty: This warranty limits our liability for the replacement of this product. No warranties of any kind, express or implied, including, without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided. BIORON Diagnostics GmbH shall have no liability for any direct, indirect, consequential, or incidental damages arising out of the use, the results of use, or the inability to use this product. Trademarks: FAM, HEX, JOE and ROX are trademarks of Applera Corporation or its subsidiaries in the US and certain other countries. iq and CFX are trademarks of Bio-Rad Laboratories, Inc. Rotor-Gene is a registered trademark of Qiagen Group, Germany. Rev _EN Page 2 of 16
3 TABLE OF CONTENT: 1. STORAGE AND TRANSPORTATION 4 2. KIT CONTENTS 4 3. INTRODUCTION 5 4. PRINCIPLES OF THE PROCEDURE 6 5. PRECAUTIONS 6 6. ADDITIONAL MATERIALS AND DEVICES REQUIRED 7 7. SPECIMEN TRANSPORT AND STORAGE 7 8. REAGENT PREPARATION 8 9. PROCEDURE PROTOCOL DATA ANALYSIS ATTACHMENT 1: CALCULATION OF VIRAL NA CONCENTRATION ATTACHMENT 2: ANALYTICAL SYSTEM VALIDATION. 15 VBD0799 Page 3 of 16
4 KIT FOR EXTRACTION OF RNA AND SUBSEQUENT REAL TIME PCR DETECTION AND QUANTITATION OF HEPATITIS C VIRUS RNA Research Use Only 1. STORAGE AND TRANSPORTATION Store assay kit at (2-8) С in the manufacturer s packing. Transportation at 25 С for 10 days is allowed. Do not freeze reagents. Shelf life of the kit as printed on the label of the outside box. Do not pool reagents from different lots or from different vials of the same lot. Strictly follow the Instruction manual for reliable results. 2. KIT CONTENTS Specimen Preparation Reagents Concentrating Solution Lysis Reagent 1 Lysis Reagent 2 Sorbent (suspension of magnetic particles) Solution for DNA/RNA Precipitation Wash Solution 1 Wash Solution 2 Specimen Diluent Control Samples Recovery Solution for Controls (RSC) Positive Control (PC) sample Negative Control (NC) sample Internal control (IC) sample, lyophilized Samples for calibration, freeze-dried (CS1 and CS2) - are used when the adequacy of analytical system has to be checked, see the Attachment 2 Passport for the Concentration of PC Amplification reagents Ready Master Mix for reverse transcription and PCR, freeze-dried (RMM) 4 vials -14 ml each 4 vials - 4 ml each 4 vials - 7 ml each 1 vial 4 vials -12 ml each 4 vials - 8 ml each 4 vials - 5 ml each 4 vials - 3 ml each 2 vials - 4 ml each 2 vial 2 vials - 12 ml each 2 vials 1 vial each 48 test tubes Rev _EN Page 4 of 16
5 3. INTRODUCTION The assay kit RealLine HCV quantitative is intended for the quantitative detection of hepatitis C virus (HCV) RNA in patient plasma and serum. The method is based on the reverse transcription of viral RNA to generate complementary DNA (cdna), with subsequent amplification of target cdna by Polymerase Chain Reaction (PCR) with fluorescent detection of amplified DNA in the real-time mode. The assay kit RealLine HCV quantitative is intended for use in conjunction with clinical practice for diagnosis of hepatitis C disease and for clinical management of HCV infected patients disease progress. The assay kit is adapted for real-time PCR detection systems like iq icycler, iq5 icycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-technology, Russia); Rotor-Gene 3000, Rotor-Gene 6000 (Corbett Research, Australia) or their analogues. The assay kit contains reagents sufficient for 4 12-test runs, which may be performed separately or simultaneously. It is strongly recommended to use three replicas of Positive Control sample and one Negative Control sample in each test run. The assay kit can be used with either of two specimen preparation procedures, the Standard Procedure or the UltraSensitive procedure. In the Standard specimen preparation procedure, HCV RNA is isolated from 100 μl of serum (plasma). In the UltraSensitive specimen preparation procedure, HCV viral particles in serum (plasma) are concentrated by Concentrating Solution of 1 ml of serum (plasma). Specificity: The assay kit is designed for in vitro determination of HCV genotypes: 1a, 1b, 2a, 2b, 2c, 2i, 3, 4, 5a, 6 regardless of subtype. The samples containing HIV RNA with concentration above the detection limit will be determined as positive. If specimen does not contain HIV RNA, analysis will give negative result (in 100 % of cases). Dynamic range of estimated concentration (linearity area): from 15 IU / ml to 10 8 IE / ml HCV RNA for the UltraSensitive procedure (or from 150 IU/ml to 10 8 IU/ml for the Standard procedure). 1 IU = 2.5 copies of HCV RNA (National Institute of Biological Standards and Control for WHO international standard for Hepatitis С virus NIBSC Code: 96/798). Sensitivity: The assay kit securely determines HCV RNA in concentration not less than 15 IU / ml for UltraSensitive specimen preparation procedure (or not less than 150 IU / ml for the Standard procedure). VBD0799 Page 5 of 16
6 4. PRINCIPLES OF THE PROCEDURE The principle of analysis is based on isolation of nucleic acids directly from serum (plasma) jointly with preliminarily added Internal Control, the reverse transcription of viral RNA with subsequent PCR amplification of target cdna by PCR with fluorescent detection of amplified DNA in the real-time mode. Quantification of Hepatitis C virus RNA is provided by application of the Positive Control (PC) in each test run. Positive Control is characterized in international units (IU/ml) by World Health Organization (WHO international standard for Hepatitis С virus NIBSC Code: 96/798), and serves as a Quantitation Standard for calculation of viral quantity. Threshold cycle value Ct is the cycle number at which fluorescence generated within a reaction crosses fluorescence threshold, a fluorescent signal significantly above the background fluorescence. Quantity of viral RNA in initial sample is calculated by comparison of the threshold cycle value of the analysed sample and Positive Control. Also efficiency of sample preparation and reverse transcription should be considered. For convenience of the user it is recommended to use a magnetic rack through workout. Specimen preparation for 1 ml or 100 μl of serum (plasma) is allowed 5. WARNING AND PRECAUTIONS For in vitro use only. The kits must be used by skilled personnel only. When handling the kit, follow the national safety requirements for working with pathogens. To prevent contamination, the stages of DNA isolation and PCR test run must be spatially separated. Avoid microbial and ribonuclease contamination of reagents when removing aliquots from reagent vials. Wear protective disposable gloves, laboratory coats and eye protection when handling specimens and kit reagents. Every workplace must be provided with its own set of variable-volume pipettes, necessary auxiliary materials and equipment. It is prohibited to relocate them to other workplaces. The use of sterile disposable pipette tips is recommended. Never use the same tips for different samples. Do not pool reagents from different lots or from different vials of the same lot. Dispose unused reagents and waste in accordance with country, federal, state and local regulations. Do not use the kit after the expiration date. Rev _EN Page 6 of 16
7 6. ADDITIONAL MATERIALS AND DEVICES REQUIRED RealLine Pathogen Diagnostic Kits Real time PCR device iq/iq5 icycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-technology, Russia); Rotor-Gene 3000/6000 (Corbet Research, Australia) or equivalent; Plates or tubes for PCR, if the uni-format tube are not suitable for the device Microcentrifuge (min RCF rpm), or equivalent; Vortex mixer; Thermo Shaker Disposable gloves, powder-free; Pipettes (capacity μl, μl) with filters (aerosol barriers); Disposable DNAse/RNase-free tips with filters; Displacement tips; 2.0 ml polypropylene tubes, sterile, non-siliconised Vacuum aspirator; 2.0 ml and 0.2 ml microtube racks; Magnetic Rack for nucleic acids isolation 7. SPECIMEN TRANSPORT AND STORAGE Attention! Specimens anticoagulated with heparin are unsuitable for this test. Blood should be collected in sterile tubes (or tubes, using EDTA or ACD as the anticoagulant). Separate serum (plasma) from whole blood within 6 hours of collection. Transfer serum (plasma) to a sterile polypropylene tube. Specimens can be transported and stored: At room temperature up to 2 hours; At (2-8) С up to 24 hours; Frozen at 20 С up to 2 weeks. Do not freeze - thaw samples repeatedly! Before use centrifuge specimens of serum (plasma) at rpm for 5 minutes at room temperature. VBD0799 Page 7 of 16
8 8. REAGENT PREPARATION 8.1. Prior to use, bring up reagents to room temperature (18-25) С for 30 minutes. Add 1 ml of Recovery Solution of Controls (RSC) into a vial with Internal Control (IC) sample, mix gently, keep for 15 minutes, then carefully mix once again. IC should be stored at (2-8) С and used within 1 month after preparation Add 1 ml of Recovery Solution for Control (RSC) into a vial with Positive Control (PC) sample, mix gently, keep for 15 minutes, then carefully mix once again. PC should be stored at (2-8) С and used within 1 month after preparation Negative Control (NC) sample is ready to use. Once opened, NC should be stored at (2-8) С and used within 1 month Prior to use, warm Lysis Reagents 1 and 2 at (50-60) С and mix thoroughly to dissolve the precipitated material. Vortex Sorbent to a condition of homogeneous suspension. Add 80 μl of Sorbent suspension into a vial with Lysis Reagent 2. Mix carefully. Attention! Once opened, any unused portion of Lysis Reagent 2 should be discarded. For the quantitative determination, the RNA extraction is conducted from 100 µl of serum (plasma) or whole blood using this kit. The Internal Control must be used with the Extraction, if another RNA Extraction kit I used, please add RealLine Internal Control (REF VBC8881) to the extraction procedure Each set of samples must contain 3 PC and 1 NC sample. The elution volume have to be 200 µl. Rev _EN Page 8 of 16
9 9. PROCEDURE PROTOCOL Ultra Sensitive procedure: RNA isolation from 1 ml of serum (plasma) 9.1. Determine the appropriate number of reaction tubes needed for patient specimen and control testing. It is recommended to use three replicas of Positive Control sample and one Negative Control sample in each test run Label each 2.0 ml tube for each patient specimen and control sample Add 30 μl of IC to each tube For NC, add to the tube, marked NC, 1000 μl of Negative Control sample For PC, add to the tube, marked PC, 970 μl of Negative Control sample, and 30 μl of Positive Control sample Add 1000 μl of each patient specimen to the appropriately labeled tube For each specimen or control tube, add 1 ml of Concentrating Solution Close the tubes and vortex for 3-5 seconds. Leave the tubes for 10 minutes at room temperature, then centrifuge for 5 minutes, 3000 rpm Using a new tip for each sample, carefully remove the supernatant without disturbing the pellet. The pellet should be clearly visible at this step Add 200 μl of Lysis Reagent 1 to each tube. Vortex vigorously for seconds. Some insoluble material may remain. Leave tubes for 5 minutes at room temperature Add 500 μl or Lysis Reagent 2 with Sorbent to each tube. Vortex for 5-10 seconds. Place the tubes into Thermo Shaker, and incubate for 10 minutes at 56 C and 1300 rpm Add 750 μl of Solution for DNA/RNA Precipitation in each tube. Vortex for seconds. Incubate 5 minutes at room temperature Centrifuge at rpm for 5 minutes at room temperature Trying not to shake up a pellet, place the tubes to Magnetic Rack. Using a new tip for each sample, carefully remove the supernatant without disturbing the pellet Add 500 μl or Wash Solution 1 in each tube. Vortex vigorously for seconds. Centrifuge at rpm for 5 minutes. VBD0799 Page 9 of 16
10 9.16. Trying not to shake up a pellet, place the tubes to Magnetic Rack. Using a new tip for each sample, carefully remove the supernatant without disturbing the pellet Add 300 μl or Wash Solution 2 to each tube. Vortex vigorously for seconds. Centrifuge at rpm for 5 minutes Trying not to shake up a pellet, place the tubes to Magnetic Rack. Using a new tip for each sample, carefully remove the supernatant without disturbing the pellet Dry the pellet with open caps for 2-3 minutes at room temperature (18-25) С Add 200 μl of Specimen Diluent to each tube. Vortex vigorously for seconds. Place the tubes into Thermo Shaker, and incubate for 10 minutes at 56 C and 1300 rpm. Then centrifuge for 1 minute at rpm Samples are ready for reverse transcription and PCR. Attention! In the case of RNA isolation start reverse transcription and PCR within 2 hours of specimen and control preparation. Isolated DNA can be stored at (2-8) С for 24 hours. Standard protocol: RNA isolation from 100 μl of serum (plasma) Determine the appropriate number of reaction tubes needed for patient specimen and control testing It is recommended to use three replicas of Positive Control sample and one Negative Control sample in each test run Label each 2.0 ml tube for each patient specimen and control sample Add 30 μl of IC to each tube For NC, add to the tube, marked NC, 100 μl of Negative Control sample For PC, add to the tube, marked PC, 70 μl of Negative Control sample, and 30 μl of Positive Control sample Add 100 μl of each patient specimen to the appropriately labeled tube Add 500 μl or Lysis Reagent 2 with Sorbent to each tube. Vortex for 5-10 seconds. Place the tubes into Thermo Shaker, and incubate for 10 minutes at 56 C and 1300 rpm Add 600 μl of Solution for DNA/RNA Precipitation in each tube. Vortex for seconds. Incubate 5 minutes at room temperature Centrifuge at rpm for 5 minutes at room temperature. Rev _EN Page 10 of 16
11 9.31. Trying not to shake up a pellet, place the tubes to Magnetic Rack. Using a new tip for each sample, carefully remove the supernatant without disturbing the pellet Add 500 μl or Wash Solution 1 in each tube. Vortex vigorously for seconds. Centrifuge at rpm for 3 minutes Trying not to shake up a pellet, place the tubes to Magnetic Rack. Using a new tip for each sample, carefully remove the supernatant without disturbing the pellet Add 300 μl or Wash Solution 2 to each tube. Vortex vigorously for seconds. Centrifuge at rpm for 3 minutes Trying not to shake up a pellet, place the tubes to Magnetic Rack. Using a new tip for each sample, carefully remove the supernatant without disturbing the pellet Dry the pellet with open caps for 2-3 minutes at room temperature (18-25) С Add 200 μl of Specimen Diluent to each tube. Vortex vigorously for seconds. Place the tubes into Thermo Shaker, and incubate for 10 minutes at 56 o C and 1300 rpm. Then centrifuge for 1 minute at rpm Samples are ready for reverse transcription and PCR. Attention! In the case of RNA isolation start reverse transcription and PCR within 2 hours of specimen and control preparation. Isolated DNA can be stored at (2-8) С for 24 hours. Reverse transcription and PCR Place the tubes with processed specimens and controls to Magnetic Rack Prepare an appropriate number of reaction tubes with Ready Master Mix (RMM). Label each reaction tube for each patient specimen and control. Attention! For iq/iq5 icycler and CFX96 put marks on the lateral part of a reaction tube. For Rotor-Gene 3000/6000 put marks on the cap on the reaction tube Add 50 μl of each processed specimen and control to the appropriately labeled reaction tube using a new RNase-free tip with aerosol barrier for each sample. Do not grasp a sorbent particles! Place reaction tubes into the thermal block of real time PCR device. VBD0799 Page 11 of 16
12 9.43. Program real time PCR device as follows: For iq/iq5 icycler, CFX96, DT-96 Stage 1: 45 С, 30 min; Stage 2: 94 С, 1 min; Stage 3: 94 C, 10 sec 50 cycles 60 C, 20 sec Fluorescence measurements should be done at 60 С. For Rotor-Gene 3000 (6000) Stage 1: 45 С, 30 min; Stage 2: 94 С, 1 min; Stage 3: 94 C, 10 sec 50 cycles 60 C, 40 sec Fluorescence measurements should be done at 60 С Collect real-time PCR data through the FAM channel for detection of amplification of IC cdna Collect real-time PCR data through the ROX channel for detection of amplification of HCV cdna. For Rotor-Gene 3000 (6000) In the New Run Wizard window click Calibrate. The window Auto Gain Calibration Setup opens. In the line Channel Settings choose ROX and FAM channels. Make sure that of Tube position is 1. Tick off Perform Calibration Before 1st Acquisition. Click Close button Record the positions of the controls and specimens According to the instruction to the used device Start the reverse transcription and PCR program. Rev _EN Page 12 of 16
13 10. DATA ANALYSIS For Rotor-Gene 3000 (6000) Results for Internal Control DNA amplification Click Analysis button, choose Quantitation from the list, choose Cycling А. FAM, click Show button Click ОK button, and cancel automatic Threshold determination Click Linear scale button In the Quantitation analysis menu buttons Dynamic tube and Slope Correct should be pressed Click More Settings (Outlier removal) button, determine NTC threshold value as 5% In the column CT Calculation (right part of the window) determine Threshold value as 0, In the result table (Quant. Results window) Ct will be displayed. Results for HCV cdna amplification Click Analysis button, choose Quantitation from the list, choose Cycling А. ROX click, Show button Click ОK button, and cancel automatic Threshold determination Click Linear scale button In the Quantitation analysis menu buttons Dynamic tube and Slope Correct should be pressed Click More Settings (Outlier removal) button, determine NTC threshold value as 5% In the column CT Calculation (right part of the window) determine Threshold value as 0, In the result table (Quant. Results window) Ct will be displayed In Positive Control sample the program should detect: ROX fluorescent signal increase and Сt value (HCV cdna amplification); FAM fluorescent signal increase and Сt value (IC cdna amplification) In Negative Control sample the program should detect: FAM fluorescent signal increase and Ct value, and no significant ROX fluorescent increase should appear. If Ct value for NC along ROX channel is less than 40, this indicates the presence of contamination The program should detect amplification signal increase for IC cdna (channel FAM) in each sample and define Ct for IC. Probe analysis is valid if Ct of IC for this sample is equal to or less than In the case of contamination (when NC is determined as positive) all positive results in this test should be repeated from the RNA extraction stage. Negative samples of such test run are considered reliable. VBD0799 Page 13 of 16
14 Calculate (IC Ct) m as the average Ct value of IC for all samples (including PC and NC). Samples with Ct of IC, that differ from (IC Ct) m by more than 2 fold, should be ignored. After screening, recalculate (IC Ct) m for remaining samples The sample is considered negative if Ct value along the ROX channel exceeds 40 or is not determined. If Ct of IC for this sample differs from (IC Ct) m by more than 2, then result for this sample should be considered as equivocal. The test should be repeated from the sample RNA extraction stage The sample is considered positive if Ct value along the ROX channel does not exceed 40. If Ct of IC for this sample differs from (IC Ct) m more then 2, the sample is considered as positive without quantitative analysis. For quantitative analysis, repeat the test beginning from the RNA isolation stage The test results are considered reliable only when Positive and Negative controls perform as expected. HCV quantitation analysis For quantitative analysis calculate the HCV RNA concentration in analyzed samples in accordance with Attachment 1. Attention! For RNA isolation from 100 μl result should be multiplied by If calculated HCV RNA concentration is in dynamic range from 100 IU / ml to 10 8 IU / ml result should be reported as positive with indication of calculated HCV RNA concentration in the sample (in IU / ml) Test results greater than 10 8 IU / ml are above the upper limit of quantitation of the Standard test and should be reported as Greater than 10 8 IU / ml Test results less than 100 IU / ml are below the lower limit of quantitation of the Standard test and should be reported as HCV RNA detected, less than 100 IU / ml Analyzed sample is considered as negative if obtains no Ct value along the ROX channel or Ct value exceed 40. For further information and help, ask us at techsupport@bioron.de. We can provide you a calculation sheet for an easy evaluation of your data. Note: For precise calculation of viral NA concentration in analyzed samples, the validation of the analytical system should be done by comparison of Positive Control PC concentration, specified in the passport of the assay kit with PC concentration, calculated using calibration graph. For this purpose three independent Positive Control samples are the needed. Rev _EN Page 14 of 16
15 11. ATTACHMENT 1: CALCULATION OF VIRAL NA CONCENTRATION. Calculate the viral NA concentration using the following equation: С sampk = С PC 2(Сt PC Сt SAMPk) 2(Сt ICk Сt ICPC), Where: k sample number; С PC PC concentration, specified in the passport of Assay kit; Сt PC and Сt IC PC Сt value of the PC sample ROX and FAM channels, accordingly; Сt SAMP k and Сt IC k Сt value of the sample numbered k along ROX and FAM channels, accordingly, if amplification efficiency is (Еа) =100 %. 12. ATTACHMENT 2: ANALYTICAL SYSTEM VALIDATION. Calculation of viral NA concentration for PC using calibration graph. For precise calculation of viral NA concentration in analyzed samples, the validation of the analytical system should be done by comparison of Positive Control PC concentration, specified in the passport of the assay kit with PC concentration, calculated using calibration graph. Prior to use, warm reagents at room temperature (18-25) С for 30 minutes. Prepare NC, PC and IC as recommended in the instruction. Add 1 ml of Solution for Restoration of Control samples (SRC) into a vial with Calibration sample 1 (CS1) and Calibration sample 2 (CS2). Mix gently, keep for 15 minutes, then carefully mix once again. For RealLine Extraction 1000 (1 ml plasma) use following amounts: Prepare ml tubes one for NC, 9 for 3 repeats of PC, CS1 and CS2. Label the tubes. Add 30 μl of IC to each tube. For NC, add to the tube, marked NC, 1 ml of Negative Control. For PC, add to each of three tubes, marked PC, 970 μl of Negative Control, and 30 μl of Positive Control. For CS1 add to each of three tubes marked CS1 970 μl of Negative Control, and 30 μl of Calibration sample 1. For CS2 add to each of three tubes marked CS2 970 μl of Negative Control, and 30 μl of Calibration sample 2. For each specimen or control tube, add 1 ml of Concentrating Solution. Run the isolation of NA as recommended in the instruction. VBD0799 Page 15 of 16
16 For RealLine Extraction 100 (100 µl plasma) use following amounts: Prepare 10 tubes one for NC, 9 for 3 repeats of PC, CS1 and CS2. Label the tubes. Add 30 μl of IC to each tube. For NC, add to the tube, marked NC, 100µl of Negative Control. For PC, add to each of three tubes, marked PC, 70 μl of Negative Control, and 30 μl of Positive Control. For CS1 add to each of three tubes marked CS1 70 μl of Negative Control, and 30 μl of Calibration sample 1. For CS2 add to each of three tubes marked CS2 70 μl of Negative Control, and 30 μl of Calibration sample 2. Run the isolation of NA as recommended in the instruction. Run the PCR (or reversed PCR). Insert concentration of CS1 and CS2, specified in the passport of Assay kit, as it recommended in the instruction of PCR instrument. For each sample calculate correct Ct value, using the following equation: Z = Сt SAMP (Сt ICm Сt IC). Calculate average Z value for PC, CS1 and CS2 (Z PCm, Z CS1m, Z CS2m). Samples with Z that differ from Zm more than 2, should be ignored. After screening, recalculate Z m for samples remained. Calculate B coefficient for calculating of specific viral NA, using following equation: B = [Lg(C CS1) Lg(C CS2)] / (Z CS2m Z CS1m), Where: C CS1 and C CS2 specific viral NA concentration in Calibration samples (specified in the passport of the assay kit); For amplification efficiency 100 % B = Lg2 = 0.3. Using received results, calculate concentration of specific viral NA for Positive Control sample, using the following analytical equation: С PC = 10 XPC (IU/ml) Where: X PC = Lg (C CS1) + B (Z CS1m Z PCm); Z CS1m average Z value for Calibration Sample 1 repeats; Z PCm average Z value for Positive Control sample repeats; If the calculated value of specific virus NA concentration in PC differs no more than in 2 from the value specified in the passport of a set, PC can be used as the sample of comparison for the further calculations of specific virus NA concentration in investigated samples. At performance of all specified conditions it is possible to spend calculation of concentration specific virus NA (in IU/ml) in investigated samples under the formula (item 11) using factor B value, calculated on calibration graph. For further information and help, ask us at techsupport@bioron.de. We can provide you a calculation sheet for an easy evaluation of your data. Rev _EN Page 16 of 16
RealLine HIV quantitative Str-Format
Instructions for use DETECTION AND QUANTIFICATION OF THE HUMAN IMMUNODEFICIENCY VIRUS RNA BY REAL TIME PCR Research Use Only (RUO) Attention! Please read the information about quantification process carefully!
More informationRealLine HCV quantitative Str-Format
Instructions for use QUANTITATIVE KIT FOR REAL TIME PCR DETECTION FOR RNA OF HEPATITIS C VIRUS Attention! Please read the information about quantification process carefully! Research Use Only (RUO) (Str
More informationRealLine HCV Qualitative Str-Format
Instructions for use REAL TIME PCR DETECTION KIT FOR THE HEPATITIS C VIRUS RNA (HCV) Research Use Only (RUO) (Str-format) VBD0795 96 Tests valid from: October 2018 Rev05_1018_EN Page 1 of 8 Explanation
More informationRealLine HIV qualitative Str-Format
Instructions for Use REAL TIME PCR DETECTION KIT FOR HUMAN IMMUNODEFICIENCY VIRUS RNA Research Use Only (RUO) RealLine HIV Qualitative (Str-format) VBD0196 96 Tests valid from July 2016 Rev01072016_EN
More informationAmpliX HBV Quantitative
Instructions for use REAL TIME PCR DETECTION AND QUANTITATION KIT OF HEPATITIS B VIRUS DNA Research Use Only (RUO) (Lyo-format) VBD0595 96 rcs valid from May 2013 Explanation of symbols used in labeling
More informationDETECTION AND QUANTITATION OF THE HUMAN IMMUNODEFICIENCY VIRUS RNA BY REAL TIME PCR
Lyo-Format Instructions for use Lyo-Format DETECTION AND QUANTITATION OF THE HUMAN IMMUNODEFICIENCY VIRUS RNA BY REAL TIME PCR (Lyo-format) 5645 96 rcs valid from May 2013 Distribué par / Distributed by:
More informationRealLine Mycoplasma genitalium Str-Format
Instructions for use ASSAY KIT FOR THE QUALITATIVE DETECTION OF MYCOPLASMA GENITALIUM DNA BY REAL-TIME PCR METHOD In vitro Diagnostics () VBD4396 96 Tests valid from December 2018 Rev06_1218_EN Page 1
More informationRealLine HBV / HCV / HIV Str-Format
Instructions for use ASSAY KIT FOR THE QUALITATIVE AND DIFFERENTIAL DETECTION OF HEPATITIS B VIRUS DNA, HEPATITIS C VIRUS RNA, AND HUMAN IMMUNODEFICIENCY VIRUS TYPES 1 AND 2 RNA USING THE PCR/RT-PCR METHOD
More informationReal Time Kit. Pneumocystis jirovecii (carinii) Real-TM Handbook. for the detection of Pneumocystis jirovecii (carinii) in biological materials
IVD For in Vitro Diagnostic Use Pneumocystis jirovecii (carinii) Real-TM Handbook Real Time Kit for the detection of Pneumocystis jirovecii (carinii) in biological materials REF P2-50FRT VER 07.02.2010
More informationCMV FEP ALA For nucleic acid amplification of CMV DNA and Fluorescence detection with End Point analysis (FEP) on Aladin (Sacace)
REF TV7-00FRT VER 3.0.06 CMV FEP ALA For nucleic acid amplification of CMV DNA and Fluorescence detection with End Point analysis (FEP) on Aladin (Sacace) Key to symbols used REF List Number Store at 2-8
More informationHDV Real-TM. Handbook
HDV Real-TM Handbook for use with RotorGene 3000/6000 (Corbett Research), SmartCycler (Cepheid), iq icycler and iq5 (Biorad), Applied Biosystems 7300/7500 Real Time PCR Systems (Applera), MX3000P and MX3005P
More informationFor in vitro Veterinary Diagnostics only. Kylt Rotavirus A. Real-Time RT-PCR Detection.
For in vitro Veterinary Diagnostics only. Kylt Rotavirus A Real-Time RT-PCR Detection www.kylt.eu DIRECTION FOR USE Kylt Rotavirus A Real-Time RT-PCR Detection A. General Kylt Rotavirus A products are
More informationHBV Real-TM Quant Handbook
HBV Real-TM Quant Handbook Real Time Kit for the Quantitative detection of Hepatitis B Virus in human plasma for use with SmartCycler (Cepheid), RotorGene 3000/6000 (Corbett Research), iq icycler and iq5
More informationHIV DNA Qual FRT Real Time Kit
REF TR-V1-D HIV DNA Qual FRT Real Time Kit Key to symbols used List Number Store at 2-8 C/-20 C RUO For Research Use Only Caution! Lot Number VER Version Expiration Date Negative Control Contains reagents
More informationCMV Real-TM Quant. REF List Number Store at 2-8 C/-20 C. RUO For Research Use Only Caution! LOT Lot Number VER Version.
REF TV7-100/2 FRT CMV Real-TM Quant Real Time Kit for use with SmartCycler (Cepheid), RotorGene 3000/6000 (Corbett Research), Applied Biosystems 7300/7500 Real Time PCR Systems (Applera), iq icycler and
More informationPneumocystis Carinii Real Time PCR Kit. For In Vitro Diagnostic Use Only User Manual
Revision No.: ZJ0003 Issue Date: Aug 7 th, 2008 Pneumocystis Carinii Real Time PCR Kit Cat. No.: QD-0082-02 For use with ABI Prism 7000/7300/7500/7900; Smart CyclerII; icycler iq 4/iQ 5; Rotor Gene 2000/3000;
More informationLeukemia BCR-ABL Fusion Gene Real Time RT-PCR Kit
Revision No.: ZJ0003 Issue Date: Aug 7 th, 2008 Leukemia BCR-ABL Fusion Gene Real Time RT-PCR Kit Cat. No.: TR-0126-02 For use with ABI Prism 7000/7300/7500/7900(96 well); Smart Cycler II; icycler iq 4/iQ
More informationInstructions for Use. RealStar Influenza Screen & Type RT-PCR Kit /2017 EN
Instructions for Use RealStar Influenza Screen & Type RT-PCR Kit 4.0 05/2017 EN RealStar Influenza Screen & Type RT-PCR Kit 4.0 For research use only! (RUO) 164003 INS-164000-EN-S01 96 05 2017 altona
More informationaltona RealStar Instructions for Use RealStar CMV PCR Kit /2017 EN DIAGNOSTICS
altona DIAGNOSTICS Instructions for Use RealStar CMV PCR Kit 1.2 08/2017 EN RealStar RealStar CMV PCR Kit 1.2 For research use only! (RUO) 021202 INS-021200-EN-S01 48 08 2017 altona Diagnostics GmbH Mörkenstr.
More informationHDV Real-TM Quant Handbook
HDV Real-TM Quant Handbook Real Time PCR Kit for the Quantitative detection of Hepatitis D Virus in human plasma for use with Real Time PCR instruments, like RotorGene 3000/6000/Q (Corbett Research, Qiagen),
More informationInstructions for Use. RealStar Influenza S&T RT-PCR Kit /2017 EN
Instructions for Use RealStar Influenza S&T RT-PCR Kit 3.0 01/2017 EN RealStar Influenza S&T RT-PCR Kit 3.0 For research use only! (RUO) 163003 INS-163000-EN-S02 96 01 2017 altona Diagnostics GmbH Mörkenstr.
More informationHIV-1 Viral Load Real Time (RG)
-1 Viral Load Real Time (RG) Real Time RT-PCR type 1 RNA quantification assay MSP Reg. pending Valdense 3616. 11700. Montevideo. Uruguay. phone (598) 2 336 83 01. Fax (598) 2 336 71 60. Info@atgen.com.uy
More informationNorgen s HIV proviral DNA PCR Kit was developed and validated to be used with the following PCR instruments: Qiagen Rotor-Gene Q BioRad icycler
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com HIV Proviral DNA PCR Kit Product # 33840 Product Insert Background Information
More informationHandbook. Influenza A H1N1 & H3N2 Real-TM. Real Time Amplification test for the detection of Influenza A H1N1 and H3N2 viruses
Influenza A H1N1 & H3N2 Real-TM Handbook Real Time Amplification test for the detection of Influenza A H1N1 and H3N2 viruses REF V54-50FRT REF TV54-50FRT 50 NAME Influenza A H1N1 & H3N2 Real-TM INTENDED
More informationMorinaga Mouse C-peptide ELISA Kit
Morinaga Mouse C-peptide ELISA Kit For the quantitative determination of C-peptide in mouse serum, plasma, and fluid 96wells For Laboratory Use Only, not for use in diagnostic procedure Please read full
More information1. Intended Use New Influenza A virus real time RT-PCR Panel is used for the detection of universal influenza A virus, universal swine Influenza A vir
New Influenza A Virus Real Time RT-PCR Kit User Manual LT028310RRFY - 1 - 1. Intended Use New Influenza A virus real time RT-PCR Panel is used for the detection of universal influenza A virus, universal
More informationProduct # Kit Components
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Pneumocystis jirovecii PCR Kit Product # 42820 Product Insert Background Information
More informationPneumocystis jirovecii (carinii) Real-TM
IVD For in Vitro Diagnostic Use Pneumocystis jirovecii (carinii) Real-TM Handbook Real Time PCR Kit for the detection of Pneumocystis jirovecii (carinii) in biological materials REF P2-50FRT REF TP2-50FRT
More informationAquaPreserve DNA/RNA/Protein Order # Preservation and Extraction Kit 8001MT, 8060MT
AquaPreserve DNA/RNA/Protein Order # Preservation and Extraction Kit 8001MT, 8060MT MoBiTec GmbH 2014 Page 2 Contents 1. Description... 3 2. Kit Contents... 3 3. Terms & Conditions... 3 4. AquaPreserve
More informationMicrosart Calibration Reagent
Instructions for Use Microsart Calibration Reagent Prod. No. SMB95-2021 Mycoplasma arginini Prod. No. SMB95-2022 Mycoplasma orale Prod. No. SMB95-2023 Mycoplasma gallisepticum Prod. No. SMB95-2024 Mycoplasma
More informationAmpliSens EBV / CMV / HHV6-screen-FRT PCR kit
For Professional Use Only AmpliSens EBV / CMV / HHV6-screen-FRT PCR kit Instruction Manual AmpliSens Ecoli s.r.o., Studenohorska 12 841 03 Bratislava 47 Slovak Republic Tel.: +421 2 6478 9336 Fax: +421
More informationNorgen s HIV Proviral DNA PCR Kit was developed and validated to be used with the following PCR instruments: Qiagen Rotor-Gene Q BioRad T1000 Cycler
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com HIV Proviral DNA PCR Kit Product# 33840 Product Insert Intended
More informationBioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:
Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions
More information(DNA) Real-time PCR. Exicycler 96 Rotor-Gene Q/6000 PCR
Real-Time (DNA) Real-time Exicycler 96 Rotor-Gene Q/6000 IU Mix1 Mix2 IU/μl IU/μl IU/μl IU/μl IU/μl IPC NTC C 1 Lot# 2 Freeze & thawing 1 MSDS: Material Safety Data Sheets Real- (TaqMan time ' FAM ' BHQ1
More information(DNA) Real-time PCR. Exicycler 96 Rotor-Gene Q/6000 PCR
Real-Time (DNA) Real-time Exicycler 96 Rotor-Gene Q/6000 IU Mix1 Mix2 IU/μl IU/μl IU/μl IU/μl IU/μl IPC NTC C 1 Lot# 2 Freeze & thawing 1 MSDS: Material Safety Data Sheets (TaqMan Real-time ' FAM ' BHQ1
More informationMouse C-Peptide ELISA Kit
Mouse C-Peptide ELISA Kit Cat.No: DEIA4507 Lot. No. (See product label) Size 96T Intended Use The Mouse C-Peptide ELISA kit is for the quantitative determination of c-peptide in mouse serum, plasma, and
More informationAmoyDx TM BRAF V600E Mutation Detection Kit
AmoyDx TM BRAF V600E Mutation Detection Kit Detection of V600E mutation in the BRAF oncogene Instructions For Use Instructions Version: B3.1 Date of Revision: April 2012 Store at -20±2 o C 1/5 Background
More informationKit Components Product # EP42720 (24 preps) MDx 2X PCR Master Mix 350 µl Cryptococcus neoformans Primer Mix 70 µl Cryptococcus neoformans Positive
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cryptococcus neoformans End-Point PCR Kit Product# EP42720 Product
More informationHPV High Risk Screen Real-TM Quant
For in Vitro Diagnostic Use HPV High Risk Screen Real-TM Quant HANDBOOK Real Time PCR kit for quantitative detection of Human Papillomavirus (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59) REF V31-100/2FRT
More informationSputum DNA Collection, Preservation and Isolation Kit 50 Individual Devices
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Sputum DNA Collection, Preservation and Isolation Kit 50 Individual
More informationABIOpure TM Viral (version 2.0)
ABIOpure TM Viral (version 2.0) DNA/RNA Extraction Handbook Cat No: M561VT50 FOR RESEARCH USE ONLY Table of Contents Contents Page Kit Components 3 Precautions 3 Stability & Storage 4 General Description
More informationXTRAKT FFPE Kit / Manual
XTRAKT FFPE Kit / Manual For manual extraction of RNA, mirna and/or DNA from Formalin Fixed Paraffin Embedded (FFPE) tissue samples Catalog No. # XTK2.0-96 For research use only. Not intended for diagnostic
More informationMolecularMD. One-Step qrt-pcr BCR-ABL Kit. Product Description and User Manual. For Quantitative RT-PCR Analysis of BCR-ABL. Contact Us.
Contact Us If you have any questions for or comments about MolecularMD, please feel free to contact us. Email Customer Service CustomerService@MolecularMD.com Technical Support TechSupport@MolecularMD.com
More informationManual innuprep DNA/RNA Virus PLUS Kit - KFFLX
Manual Order No.: 845-.)- reactions 845-.)- reactions Publication No.: HB_KF-5196_e_150414 This documentation describes the state at the time of publishing. It needs not necessarily agree with future versions.
More informationE.Z.N.A. SQ Blood DNA Kit II. Table of Contents
E.Z.N.A. SQ Blood DNA Kit II Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Blood Storage and DNA Yield...4 Preparing Reagents...5 100-500 μl Whole Blood Protocol...6
More informationRat Hemoglobin A1c (HbA1c) Kit Instructions
V.3 Crystal Chem Rat Hemoglobin A1c (HbA1c) Kit Instructions For the quantitative determination of hemoglobin A1c (HbA1c) in rat whole blood Catalog #80300 96 Assays For research use only. Not for use
More informationAmpliSens Influenza virus A H5N1-FRT PCR kit
For Professional Use Only AmpliSens Influenza virus A H5N1-FRT PCR kit Instruction Manual AmpliSens Ecoli s.r.o., Studenohorska 12 841 03 Bratislava 47 Slovak Republic Tel.: +421 2 6478 9336 Fax: +421
More informationExiPrep TM Viral DNA/ RNA Kit
Ver. 2.0 ExiPrep TM Viral DNA Kit (K-3515) ExiPrep TM Viral RNA Kit (K-3525) (K-3535) Safety Warnings and Precautions is developed and sold for research purposes only. It is not recommended for human or
More informationPoliovirus Real-TM Handbook
Poliovirus Real-TM Handbook for use with RotorGene 3000/6000 (Corbett Research), SmartCycler (Cepheid), iq icycler and iq5 (Biorad), Applied Biosystems 7300/7500 Real Time PCR Systems (Applera), MX3000P
More informationWHO Prequalification of Diagnostics Programme PUBLIC REPORT
WHO Prequalification of Diagnostics Programme PUBLIC REPORT Product: COBAS AmpliPrep/COBAS TaqMan HIV-1 Test, version 2.0 (TaqMan 48) Number: PQDx 0126-046-00 Abstract The COBAS AmpliPrep/COBAS TaqMan
More informationCytomegalovirus (CMV) End-Point PCR Kit Product# EP36300
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cytomegalovirus (CMV) End-Point PCR Kit Product# EP36300 Product
More informationExiPrep Plus Viral DNA/RNA Kit User s Guide ExiPrep Plus Viral DNA/RNA Kit ExiPrep Plus Viral DNA Kit ExiPrep Plus Viral RNA Kit
User s Guide ExiPrep Plus Viral DNA/RNA Kit ExiPrep Plus Viral DNA Kit ExiPrep Plus Viral RNA Kit K-4271 K-4272 K-4273 ExiPrep Plus Viral DNA/RNA Kit (K-4271) ExiPrep Plus Viral DNA Kit (K-4272) ExiPrep
More informationMidi Plant Genomic DNA Purification Kit
Midi Plant Genomic DNA Purification Kit Cat #:DP022MD/ DP022MD-50 Size:10/50 reactions Store at RT For research use only 1 Description: The Midi Plant Genomic DNA Purification Kit provides a rapid, simple
More informationIV2-113E Use by. Invitron Glargine ELISA Kit REF LOT IVD. Definitions. English. For in-vitro diagnostic use. Instructions for use.
Definitions Instructions for use REF Catalogue number IV2-113E Use by English Invitron Glargine ELISA Kit For in-vitro diagnostic use Σ 96 LOT IVD Lot/Batch Code Storage temperature limitations In vitro
More informationStorage: Logix Smart Zika Virus Master Mix and Logix Smart Zika Virus Positive Control must be stored at -20 ⁰C and can last up to 60 days.
Logix Smart Zika Virus (ZIKV-K-003; ZIKV-PC-003; GEN-NTC-001) Description The Logix Smart Zika Virus Test developed by Co-Diagnostics, Inc. detects ribonucleic acid (RNA) of Zika Virus in a single step
More informationMALDI Sepsityper Kit
Instructions for Use MALDI Sepsityper Kit Kit for identification of microorganisms from positive blood cultures using the MALDI Biotyper system CARE products are designed to support our worldwide customers
More informationaltona DIAGNOSTICS altona DIAGNOSTICS RealStar CMV PCR Kit 1.0 always a drop ahead. 11/2012
altona DIAGNOSTICS altona DIAGNOSTICS RealStar CMV PCR Kit 1.0 11/2012 altona Diagnostics GmbH Moerkenstr. 12 22767 Hamburg Germany phone +49 40 548 06 76-0 fax +49 40 548 06 76-10 e-mail info@altona-diagnostics.com
More informationProduct Contents... 1 Specifications... 1 Product Description... 2 Buffer Preparation... 3 Protocol Appendix... 5 Ordering Information...
INSTRUCTION MANUAL Quick-DNA/RNA Pathogen MagBead Catalog Nos. R2145 & R2146 Highlights High-throughput, magnetic-bead based purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety
More informationFor Research Use Only Ver
INSTRUCTION MANUAL ZR Viral RNA Kit Catalog Nos. R1034 & R1035 Highlights Quick, 5-minute recovery of viral RNA from plasma, serum and other samples. Omits the use of organic denaturants and proteases.
More informationPhosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for use with the PrimePCR Reverse Transcription Control Assay
Catalog # Description 172-5080 SingleShot Cell Lysis Kit, 100 x 50 µl reactions 172-5081 SingleShot Cell Lysis Kit, 500 x 50 µl reactions For research purposes only. Introduction The SingleShot Cell Lysis
More informationCoQ10(Coenzyme Q10) ELISA Kit
CoQ10(Coenzyme Q10) ELISA Kit Catalogue No.: EU0196 Size: 48T/96T Reactivity: Universal Detection Range: 0.781-50ng/ml Sensitivity:
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-DNA/RNA Pathogen Miniprep Catalog Nos. R1042 & R1043 Highlights Spin-column purification of pathogen (virus, bacteria, protozoa) DNA/RNA from a wide variety of vectors (mosquitoes,
More informationMouse Leptin ELISA Kit Instructions
V.6/Mar/2008 CRYSTAL CHEM INC. Mouse Leptin ELISA Kit Instructions For the quantitative determination of leptin in mouse serum or plasma and fluid Catalog #: 90030 96 Assays For Research Use Only. Not
More informationEBV Real-TM Quant Handbook
IVD For in Vitro Diagnostic Use EBV Real-TM Quant Handbook Real Time PCR Kit for quantitative detection of Epstein Barr Virus (EBV) for use with RotorGene 3000/6000 (Corbett Research), SmartCycler (Cepheid),
More informationRoche Molecular Biochemicals Application Note No. HP 1/1999
Roche Molecular Biochemicals Application Note No. HP 1/1999 Nucleic Acid Purification High Pure Viral Nucleic Acid Kit High Pure 16 System Viral Nucleic Acid Kit Efficiency of Hepatitis C Virus sample
More informationVitamin A-E Serum HPLC Analysis Kit
Vitamin A-E Serum HPLC Analysis Kit ZV-4013-0200-10 200 2-8 C ZIVAK TEKNOLOJI SANAYI VE TICARET LIMITED SIRKETI Gursel Mh. Eski Besiktas Cd. No:44/1-2 KAGITHANE-ISTANBUL-TURKEY www.zivak.com info@zivak.com
More informationPKU (Phenylketonuria) Serum HPLC Analysis Kit User Manual
Page 1 / 20 PKU (Phenylketonuria) Serum HPLC Analysis Kit User Manual ZV-4003-0200-10 200 2-8 C Page 2 / 20 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE... 3
More informationCarnitine / Acylcarnitines Dried Blood Spots LC-MS/MS Analysis Kit User Manual
Page 1 / 14 Carnitine / Acylcarnitines Dried Blood Spots LC-MS/MS Analysis Kit User Manual ZV-3051-0200-15 200 Page 2 / 14 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST
More informationInstructions for Use (Version 3-14 th May 2003) Changes from previous version are shown in Red, deletions are shown as ^
Page 1 of 5 Page 1 of 5, 97/656 WHO 1 st International Standard for HIV-1 RNA for Nucleic Acid-Based Techniques (NAT) NIBSC code: 97/656 Instructions for Use (Version 3-14 th May 2003) Changes from previous
More informationProduct Contents. 1 Specifications 1 Product Description. 2 Buffer Preparation... 3 Protocol. 3 Ordering Information 4
INSTRUCTION MANUAL Quick-RNA Midiprep Kit Catalog No. R1056 Highlights 10 minute method for isolating RNA (up to 1 mg) from a wide range of cell types and tissue samples. Clean-Spin column technology allows
More informationGlobal Histone H3 Acetylation Assay Kit
Global Histone H3 Acetylation Assay Kit Catalog Number KA0633 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationAmpliSens Influenza virus A-type-FRT
For Professional Use Only AmpliSens Influenza virus A-type-FRT PCR kit Instruction Manual AmpliSens Ecoli s.r.o., Studenohorska 12 841 03 Bratislava 47 Slovak Republic Tel.: +421 2 6478 9336 Fax: +421
More informationBlood Urea Nitrogen Enzymatic Kit Manual Catalog #:
BIOO LIFE SCIENCE PRODUCTS Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: 5602-01 BIOO Scientific Corp. 2010 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Description... 1 Procedure Overview...
More informationvirellatbe real time RT-PCR Kit LC
Instruction for Use virellatbe real time RT-PCR Kit LC For the in-vitro detection of TBE Virus RNA in clinical specimens and in ticks. G01065-32 G01065-96 32 96 gerbion gmbh & Co. KG Remsstr. 1 70806 Kornwestheim
More informationEPIGENTEK. EpiQuik Global Histone H3 Acetylation Assay Kit. Base Catalog # P-4008 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Global Histone H3 Acetylation Assay Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-RNA Viral Kit Catalog Nos. R1034 & R1035 Highlights Quick, spin-column purification of viral RNA from plasma, serum, CSF, cell culture media, cellular suspensions, urine, blood,
More informationEPIGENTEK. EpiQuik Global Histone H4 Acetylation Assay Kit. Base Catalog # P-4009 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Global Histone H4 Acetylation Assay Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Global Histone H4 Acetylation Assay Kit is suitable for specifically measuring global
More informationHuman Umbilical Vein Endothelial Cell Manual
Human Umbilical Vein Endothelial Cell Manual INSTRUCTION MANUAL ZBM0079.03 SHIPPING CONDITIONS Human Umbilical Vein Endothelial Cells, cryopreserved Cryopreserved cells are shipped on dry ice and should
More information25-Hydroxyvitamin D2-D3 Serum VD-200 UHPLC Analysis Kit User Manual
Page 1 / 12 25-Hydroxyvitamin D2-D3 Serum VD-200 UHPLC Analysis Kit User Manual ZV-4027-0500-10 500 2-8 C Page 2 / 12 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. IVD SYMBOLS...
More informationHuman Alpha 1 microglobulin ELISA Kit
Human Alpha 1 microglobulin ELISA Kit Catalogue No.: EH4144 Size: 48T/96T Reactivity: Human Range:0.625-40ng/ml Sensitivity:
More informationMouse Ultrasensitive Insulin ELISA
Mouse Ultrasensitive Insulin ELISA For the quantitative determination of insulin in mouse serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research Use
More informationMouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit
Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Catalogue No.: EM0002 Size: 96T Reactivity: Mouse Application: This immunoassay kit allows for the qualitative determination of HBsAg in Mouse serum
More informationFor Research Use Only Ver
INSTRUCTION MANUAL Quick-DNA/RNA Viral MagBead Catalog Nos. R2140 & R2141 Highlights High-throughput, magnetic-bead based purification of viral DNA and RNA from plasma, serum, urine, cell culture media,
More informationNuclear Extraction Kit
Nuclear Extraction Kit Item No. 10009277 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION 3 Materials
More informationHuman CD4+T Cell Care Manual
Human CD4+T Cell Care Manual INSTRUCTION MANUAL ZBM0067.04 SHIPPING CONDITIONS Human CD4+T Cells, cryopreserved Cryopreserved human CD4+T cells are shipped on dry ice and should be stored in liquid nitrogen
More informationPRODUCT: RNAzol BD for Blood May 2014 Catalog No: RB 192 Storage: Store at room temperature
PRODUCT: RNAzol BD for Blood May 2014 Catalog No: RB 192 Storage: Store at room temperature PRODUCT DESCRIPTION. RNAzol BD is a reagent for isolation of total RNA from whole blood, plasma or serum of human
More informationFor purification of viral DNA and RNA from a wide range of sample materials
QIAamp virus kits For purification of viral DNA and RNA from a wide range of sample materials Automatable on QIAGEN s proven QIAamp Kits set the standard for purification of viral DNA and RNA. QIAamp virus
More informationHuman Umbilical Cord Blood CD34 + /133+ Progenitor Cell Care Manual
Human Umbilical Cord Blood CD34 + /133+ Progenitor Cell Care Manual INSTRUCTION MANUAL ZBM0065.04 SHIPPING CONDITIONS Human Umbilical Cord Blood CD34+/133+ Progenitor Cells, cryopreserved Cryopreserved
More informationVGKC-Autoantibody Assay RIA
Instructions for Use VGKC-Autoantibody Assay RIA 125I-Radio Immuno Assay for the Quantitative Determination of Antibodies to the Voltage-Gated Potassium Channel (VGKC) in Serum I V D REF RA115/25 25 2
More informationVedolizumab Drug Level ELISA
Vedolizumab Drug Level ELISA For the quantitative determination of free Entyvio concentration in human serum and EDTA plasma. Please read carefully due to Critical Changes, e.g., Updates to specificity
More informationInfluenza A IgG ELISA
Influenza A IgG ELISA For the qualitative determination of IgG-class antibodies against Influenza A virus in human serum or plasma (citrate, heparin). For Research Use Only. Not For Use In Diagnostic Procedures.
More informationManual FTD BCE. Quantitative assay for in vitro diagnostics
Manual 32 reactions (catalog no. FTD-54.1-32) 64 reactions (catalog no. FTD-54.1-64) Quantitative assay for in vitro diagnostics For use with the ABI 7500, ABI 7500 Fast, ViiA 7, Bio-Rad CFX96, LightCycler
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture
Version 1 Last updated 14 March 2018 ab228564 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture For the measurement of human exosomes in cell culture. This product is for research use only
More informationNEPHROCHECK Calibration Verification Kit Package Insert
NEPHROCHECK Calibration Verification Kit Package Insert Manufactured for Astute Medical, Inc. 3550 General Atomics Ct. Building 2 San Diego, CA 92121 USA Intended Use The NEPHROCHECK Calibration Verification
More informationHuman HIV (1+2) antigen&antibody ELISA Kit
Human HIV (1+2) antigen&antibody ELISA Kit Catalog Number. CSB-E18042h For the qualitative determination of human HIV (1+2) antibody and P24 antigen concentrations in serum, plasma. This package insert
More informationHuman diagnostics. Better be Sure: Quantify HDV & HBV viral load. RoboGene product family
Human diagnostics Better be Sure: Quantify HDV & HBV viral load. RoboGene product family 2 RoboGene Product Family Improved patient management: Standardized monitoring of HBV DNA and HDV RNA viral load.
More informationAmpliSens Influenza virus A/B-FRT PCR kit
For Professional Use Only AmpliSens Influenza virus A/B-FRT PCR kit Instruction Manual AmpliSens Ecoli s.r.o., Studenohorska 12 841 03 Bratislava 47 Slovak Republic Tel.: +421 2 6478 9336 Fax: +421 2 6478
More informationAbbott RealTime HIV-1
E RealTime HIV-1 6L18 51-602146/R2 Abbott RealTime HIV-1 Customer Service: 1-800-553-7042 This package insert must be read carefully prior to use. Package insert instructions must be followed accordingly.
More informationPKU (Phenylketonuria) Serum LC-MS/MS Analysis Kit User Manual
Page 1 / 24 PKU (Phenylketonuria) Serum LC-MS/MS Analysis Kit User Manual ZV-3003-0200-10 200 2-8 C Page 2 / 24 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE...
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More information