Key words: G-CSF, immunocompromised host, pyelonephritis, active oxygen

Transcription

1 Key words: G-CSF, immunocompromised host, pyelonephritis, active oxygen

2 Fig. 1 Administration schedule of G-CSF and cyclophosphamide (CPA) before evaluation of chemiluminescence and induction of experimental pyelonephritis in each rat group. and evaluation of chemiluminescence of peritoneal macrophages and neutrophils at day 4. of 1 ~ 10 cfu of P. aeruginosa 18s at day 4 and observation of survival rate untill day 11. (A-P, B-P, C-P and D-P are groups of each experimental pyelonepnritis.)

3 Fig. 2 Chemiluminescence values of rat peritoneal macrophages; significant differences were seen between group A and B, or C and D by means of Student's t test. Table 1 The number of peripheral leukocytes in each group; significant difference were seen between group A and B, or A and C by means of student's t test (*p<0.05, **p<0.001) Table 2 Chemiluminescence values of peritoneal macrophages and neutrophils of each experimental group

4 Fig. 3 Chemiluminescence values of rat peritoneal neutrophils; significant differences were seen between group A and C, or C and D by means of Student's t test. Fig. 4 Survival rates of experimental pyelonephritis models; significant differences were seen between group A-P and B-P, or C-P and D-P by means of generalized Wilcoxon test. Group A-P: control (n= 15) Group B-P: G-CSF treated (n= 15) Group C-P: CPA treated (n= 15) Group D-P: CPA+G-CSF treated (n= 15)

5 Table 3 Mean survival time after inoculation of P. aeruginosa 18s; significant difference were seen between group A-P and B-P, C-P and D-P, or A-P and C-P by means of generalized Wilcoxon test (* p< 0.02, ** p< 0.001)

6 8) Bronchud, M.H., Potter, M.R., Morgenstern, G., Blasco, M.J., Scarffe, J.H., Thatcher, N., Crosther, D., Souza, L.M., Alton, N.K., Testa, N. G. & Dexter, T.M.: In vitro and in vivo analysis of the effects of recombinant human granulocyte colony-stimulating factor in patients. Br. J. Cancer, 58: 64-69, ) Welch, W.D.: Correlation between measurements of the luminol dependent chemiluminescence response and bacterial susceptibility to phagocytosis. Infec. Immun., 30: , ) Matsumoto, M., Matsubara, S., Matsuno, T., Tamura, M., Hattori, K., Nomura, H., Ono, M. & Yokota, T.: Protective effect of human granulocyte colonystimulating factor on mi- infection in neutropenic crobial mice. Infect. Immun., 55: , ) Ohsaka, A., Kitagawa, S., Sakamoto, S., Miura, Y., Takanashi, N. & Takaku, F.: In vivo activation of human neutrophil functions by 3) Allen, R.C. & Loose, D.: Phagocytic activation administration of recombinant human of a luminol dependent chemiluminescence granulocyte colony-stimulating factor in in rabbit alveolar and peritoneal macrophages. Biochem. Biophy. Res. Common., 69: , patiens with malignant lymphoma. Blood, 74: , ) Kaye, D.: The effect of water diuresis on spread of bacteria through the urinary tract. J. Infect. Dis., 124: , ) Holland, P.C., Pratt, L. & Ryan, M.P. Cisplatin induced nephrotoxicity, hypomagnesaemia and renal magnesium wasting in young rats ; a role for magnesium supplementation in attenuating nephrotoxicity. J. Med. Sci., 159(1): 33-34, ) Ushijima, Y., Nakano, M.: Kinetic aspects of luminescence in activated leukocyte system. J. Apply. Biochem., 2: , 1980.

7 Influence of Granulocyte Colony-Stimulating Factor on Bactericidal Activities of Macrophages and Polymorphonuclear Leukocytes Motoshi KAWAHARA Department of Urology, The Jikei University, School of Medicine (Director: Prof. Toyohei MACHIDA, M. D.) We investigated the influence of granurocyte colony-stimulating factor (G-CSF) on bactericidal activities of macrophages and polymorphonuclear leukocytes (PMNs) from experimental pyelonephritis in leukocytopenic rats, in order to clarify the usefulness of G-CSF for opportunistic pyelonephritis. We prepared three groups of experimental pyelonephritis, i. e., G-CSF administration group (group- 1), cyclophosphamide (CPA) administration group (group-2), and CPA and G-CSF administration group (group-3). And we measured the active oxygen generation of peritoneal macrophages and PMNs in each group. On the other hand, we produced pseudomonal pyelonephritis in each group, and compared the survival rate of each group for 7 days. G-CSF enhanced active oxygen generation of peritoneal macrophages and PMNs, significantly. Furthermore, G-CSF improved the survival rate of pseudomonal pyelonephritis in leukocytopenic rats. These results indicated that G-CSF enhanced bactericidal activities of macrophages and PMNs in vivo, and prevents dissemination of infections.