http / / cjbmb. bjmu. edu. cn Chinese Journal of Biochemistry and Molecular Biology COX-2 NTera-2 NTera-2 RT-PCR FasL caspase-8 caspase-3 PARP.

Size: px
Start display at page:

Download "http / / cjbmb. bjmu. edu. cn Chinese Journal of Biochemistry and Molecular Biology COX-2 NTera-2 NTera-2 RT-PCR FasL caspase-8 caspase-3 PARP."

Transcription

1 ISSN CN / Q http / / cjbmb bjmu edu cn Chinese Journal of Biochemistry and Molecular Biology ~ 636 NTera-2 ** ** * COX-2 NTera-2 MTT NTera-2 NTera-2 Hoechest Annexin V-FITC / PI NTera-2 RT-PCR NTera-2 Fas caspase-8 FasL caspase-8 caspase-3 PARP caspase Z-VAD-FMK NTera-2 NTera-2 caspase NTera-2 NTera-2 Q789 Aspirin Induces Apoptosis in Cultured NTera-2 Human Malignant Testicular Germ Cells YI Duo ** LI Xiao-Feng ** ZHAO Xiao-Meng WANG Cheng ZHANG Xiao-Ting LIU Xi-Zhi ZHOU Chang * Department of Biochemistry and Molecular Biology Key Laboratory of Protein Chemistry and Developmental Biology Ministry of Education of China College of Life Sciences Hunan Normal University Changsha China Abstract Aspirin also known as acetylsalicylic acid has been shown to have anticancer effects with multiple activities including inhibition of proliferation induction of apoptosis and inhibition of angiogenesis The functional characteristics and the anti-tumor mechanism of aspirin are largely unknown except for the suppression of cyclo-oxygenase 2 COX-2 Aspirin administration is known to associate with lower risks of many cancer types but less reported in human testicular tumors We investigated on whether aspirin was able to induce apoptosis in NTera-2 human malignant testicular germ cells using MTT to detected cell viabilities The morphological changes formation of apoptotic bodies and cell apoptosis were assessed by fluorescence microscopy Hoechest33342 staining Annexin V-FITC / PI flow cytometry RT-PCR and Western blotting were also performed The result showed that aspirin No No No K * Tel ssipzc1995@ yahoo com cn ** Received April Accepted May Supported by National Natural Science Foundation of China No No Project of Changsha Science and Technology Plan No K * Corresponding author Tel ssipzc1995@ yahoo com cn ** Two authors contributed equally to this work

2 7 NTera inhibited the proliferation of NTera-2 cells in a dose- and time-dependent manner and Fas and caspase- 8 were up-regulated The level of FasL was down-regulated after aspirin treatment The protein levels of caspase-3 caspase-8 and poly ADP-Ribose polymerase PARP were increased Caspase inhibitor Z- VAD-FMK could attenuate the aspirin induced apoptosis We concluded that the anticancer activity of aspirin was partially due to the inhibition of cell viability and induction of apoptosis associated with caspase apoptotic pathway Key words aspirin NTera-2 cell cell apoptosis caspase 90% NTera-2 1 Hoechst Sigma PARP caspase- 20 ~ caspase-3 Fas Santa Cruz FasL Abcam Caspase Z-VAD- 50 FMK Promega 6 /10 ~ 11 /10 3 GAR GAM Jackson Immuno Research Annexin V-FITC BD Pharmingen caspase 8 80% MTT NTera-2 D h 4 h 5 mg / ml MTT 50 μl 450 μl MTT 0 5 mg / ml MTT μl DMSO 10 min nm A 1 3 Hoechest mmol / L aspirin 24 h PBS ml / 4% 30 min PBS 2 50% / 30 s 50% 2011 Rothwell 6 Lancet / 1 ml PBS 5 min 4 Hoechest min 1 ml 7-12 PBS 3 5 min Zeiss Axioskop mmol / L NTera-2 PBS 37 5% CO NTera h Annexin-Ⅴ / PI PBS μl 1 5 μl Annexin-Ⅴ 10 μl PI

3 min 400 μl Z-VAD-FMK Annexin-V PI 20 μmol / L Caspase Caspase-8 pna Western PARP μl 20 min g 4 15 min caspase NTera-2 90 μl 2 mmol / L Ac-IETD-pNA 10 μl 80 μl 10 μl 2 mmol / L Ac-DEVD-pNA 10μL caspase RT-PCR Fas Caspase-8 30 s s 30 Table 1 The primer sequences and the product lengths of semi-quantitative RT-PCR Primer Primer sequence Product / bp Fas F 5'-GCATCTGGACCCTCCTACCT-3' 210 Fas R 5'-CTTGGTGTTGCTGGTGAGTG-3' Caspase8 F 5'-GGAGGAGTTGTGTGGGGTAA-3' 205 Caspase8 R 5'-TGCATCCAAGTGTGTTCCAT-3' GAPDH F 5'-GACTGTCTCCTCCCAAATTT-3' 496 GAPDH R 5'-GCATGGACTGTGGTCATGAGT-3' 1 7 Western mmol / L Caspase-8 Caspase-8 Ac-IETD-pNA NTera-2 PBS 2 RIPA caspase-8 20 min g 20 min caspase-8 30 ~ 40 μg SDS-PAGE caspase-8 PVDF 5% Fig 4 FasL PARP caspase-3 caspase-8 β caspase h 1 h mmol / L 6 DAB 2 4 FasL caspase 3 Z-VAD-FMK NTera-2 30 min 24 h MTT NTera-2 Fig 1 10 mmol / L NTera h 24% 51% 79% 82% 2 2 NTera-2 mrna TRIZOL h mmol / L NTera-2 RNA 1 15 μg RNA DNase Promega mmol / L Table 1 RT-PCR GAPDH 20 mmol / L PCR 94 4 min s mmol / L Fig 2 Annexin V PI NTera- 2 NTera- 2 Fig 3A Annexin V PI NTera mmol / L aspirin NTera-2 24 h 8 76% 16 37% 33 67% 36 85% 47 79% 5 07% Fig 3B 2 3 caspase-8 pna 13 pna 405 nm mmol / L

4 7 NTera Fig 1 Aspirin inhibits cell viability of NTera-2 cells by a time- and dose-dependent manner NTera-2 cells were incubated with mmol / L aspirin respectively for 32 hours And cell growth was determined by the MTT assay every 8 hours ** P < 0 01 vs control group * P < 0 05 NTera-2 TRIZOL RNA RT-PCR Fas caspase-8 mrna Fig 5A Western NTera-2 FasL caspase-8 caspase-3 pro-caspase 8 pro-caspase 3 10 mmol / L PARP Fig 5B 2 5 Z-VAD-FMK Z-VAD-FMK caspase caspase Z-VAD-FMK NTera-2 30 min 24 h Fig 6A Fig 2 The morphological change of NTera-2 cell apoptosis induced by aspirin Induction of apoptosis in NTera-2 cells by treatment with mmol / L aspirin respectively for 24 hours The treated cells were stained with Hoechst and the apoptotic morphological changes in the nuclear chromatin were observed under a fluorescent microscope The apoptosis cells were indicated as arrows magnification 40 NTera-2 PARP 8 h PARP Z-VAD-FMK caspase NTera-2 NTera-2 15 mmol / L 3 NTera-2 4 h 8 h 1 Western Fig 6B 4 h PARP 8 h

5 Fig 3 Annexin V / PI analysis of apoptotic cells in aspirin-treated cells NTera-2 cells were treated for 24 hours at concentration of mmol / L aspirin or remained PBS-treated as a control A Representative data of flow cytometry assay Percentage of apoptosis was denoted on top of the dot plots and was counted by annexin V-positive / propidium iodide-negative cells B Each bar corresponded to the mean ± SD for three independent experiments Fig 4 Caspase-8 protease activity detected in aspirintreated NTera-2 cells NTera-2 cells were treated with different concentrations of aspirin Cells were harvested after 24 hours and caspase-8 protease activity was measured using a colorimetric caspase-8 activity assay kit The values were the mean the standard deviation of triplicate assays Annexin V-FITC / PI NTera-2 14 Fas Fas FasL Fas Fas Fas associated death domain FADD caspase-8 Fas / FasL DISC 15 caspase-8 caspase caspase-3 MTT NTera-2 24 h Fas caspase-8 NTera-2 mrna Fas FasL

6 7 NTera Fig 5 Aspirin modulates expression of apoptotic genes in NTera-2 cells Cells were treated with different concentrations of aspirin for 24 hours as indicated A The expression of Fas and caspase-8 in NTera-2 cells treated with aspirin were analyzed by RT-PCR GAPDH was used as control B Cells were lysed in RIPA lysis buffer FasL pro-caspase-8 pro-caspase-3 and PARP expression were assayed by Western blotting Expression was normalized with anti-β-actin One representative of three different experiments was showed Images were converted to gray scale and analyzed using Image J software FasL caspase Fas / FasL NTera-2 References caspase Caspase Urology Fas FasL FADD caspase-8 DED caspase-8 caspase NTera-2 FasL pro-caspase 8 pro-caspase 3 caspase-8 caspase-3 PARP PARP Z-VAD-FMK NTera-2 PARP Fig 6 Aspirin activates caspase-mediated apoptosis signaling pathway A NTera-2 cells were exposed to mmol / L aspirin respectively for 24 hours after treated with caspase inhibitor Z-VAD-FMK for 30 minutes The whole cell extracts were harvested and examined with anti-parp and anti-β-actin antibody by Western blotting B NTera-2 cells were incubated with 15 mmol / L aspirin in indicated times but not treated with Z- VAD-FMK Cells were harvested and the expression of PARP protein was analyzed by Western blotting β-actin was showed as an internal standard One representative of three different experiments was showed NTera-2 caspase NTera-2 1 Hara S Miyake H Nakamura I et al Increased Fas ligand expression in the tumor tissue and serum of patients with testicular germ cell tumors with seminomatous elements J 2 Horwich A Shipley J Huddart R J Lancet Ginsburg J Testicular germ-cell cancer Unanswered questions in carcinoma of the testis J Lancet Einhorn L H Curing metastatic testicular cancer J Proc Natl Acad Sci U S A Kollmannsberger C Kuzcyk M Mayer F et al Late toxicity following curative treatment of testicular cancer J Semin Surg Oncol Rothwell P M Fowkes F G Belch J F et al Effect of daily aspirin on long-term risk of death due to cancer analysis of individual patient data from randomised trials J Lancet

7 Gu Q Wang J D Xia H H et al Activation of the caspase-8 / Bid and Bax pathways in aspirin-induced apoptosis in gastric cancer J Carcinogenesis Kong S Y Kang J H Kwon Y et al Serum HER-2 concentration in patients with primary breast cancer J J Clin Pathol Merrick D T Kittelson J Winterhalder R et al Analysis of c- ErbB1 / epidermal growth factor receptor and c-erbb2 / HER-2 expression in bronchial dysplasia evaluation of potential targets for chemoprevention of lung cancer J Clin Cancer Res Pt Maxwell P H Tumor strengths and frailties Aspiring to prevent colon cancer J Nat Med Greenberg E R Baron J A Freeman D H Jr et al Reduced risk of large-bowel adenomas among aspirin users The Polyp Prevention Study Group J J Natl Cancer Inst Hossain M A Kim D H Jang J Y et al Aspirin induces apoptosis in vitro and inhibits tumor growth of human hepatocellular carcinoma cells in a nude mouse xenograft model J Int J Oncol Villa P Kaufmann S H Earnshaw W C Caspases and caspase inhibitors J Trends Biochem Sci Ashkenazi A Dixit V M Death receptors signaling and modulation J Science Chinnaiyan A M Tepper C G Seldin M F et al FADD / MORT1 is a common mediator of CD95 Fas / APO-1 and tumor necrosis factor receptor-induced apoptosis J J Biol Chem Oliver F J de la Rubia G Rolli V et al Importance of poly ADP-ribose polymerase and its cleavage in apoptosis Lesson from an uncleavable mutant J J Biol Chem Suhara T Kim H S Kirshenbaum L A et al Suppression of Akt signaling induces Fas ligand expression involvement of caspase and Jun kinase activation in Akt-mediated Fas ligand regulation J Mol Cell Biol

The effect of elemene reversing the multidurg resistance of A549/DDP lung cancer cells

The effect of elemene reversing the multidurg resistance of A549/DDP lung cancer cells 213 12 33 12 TUMOR Vol. 33, December 213 www.tumorsci.org 161 Basic Research DOI: 1.3781/j.issn.1-7431.213.12. 5 Copyright 213 by TUMOR A549/DDP 1, 2 2 1 3 4 1 1 1. 3619 2. 355 3. 3611 4. 361 elemene cisplatin

More information

(A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and a

(A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and a Supplementary figure legends Supplementary Figure 1. Expression of Shh signaling components in a panel of gastric cancer. (A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and

More information

To determine the effect of over-expression and/or ligand activation of. PPAR / on cell cycle, cell lines were cultured as described above until ~80%

To determine the effect of over-expression and/or ligand activation of. PPAR / on cell cycle, cell lines were cultured as described above until ~80% Supplementary Materials and Methods Cell cycle analysis To determine the effect of over-expression and/or ligand activation of PPAR / on cell cycle, cell lines were cultured as described above until ~80%

More information

Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy

Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy Jianhua Chen, Pei Gao, Sujing Yuan, Rongxin Li, Aimin Ni, Liang Chu, Li Ding, Ying Sun, Xin-Yuan Liu, Yourong

More information

http / /cjbmb. bjmu. edu. cn Chinese Journal of Biochemistry and Molecular Biology A431 . Western aza-dC FUT4-siRNA

http / /cjbmb. bjmu. edu. cn Chinese Journal of Biochemistry and Molecular Biology A431 . Western aza-dC FUT4-siRNA ISSN 1007-7626 CN 11-3870 / Q http / /cjbmb bjmu edu cn Chinese Journal of Biochemistry and Molecular Biology 2015 8 31 8 836 ~ 842 DOI 10 13865 /j cnki cjbmb 2015 08 09 FUT4-siRNA 5-aza-dC 1 3 * 1 1 3

More information

Li et al. Journal of Experimental & Clinical Cancer Research (2018) 37:108

Li et al. Journal of Experimental & Clinical Cancer Research (2018) 37:108 Li et al. Journal of Experimental & Clinical Cancer Research (2018) 37:108 https://doi.org/10.1186/s13046-018-0774-7 CORRECTION Correction to: Novel smac mimetic APG- 1387 elicits ovarian cancer cell killing

More information

p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO

p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO Supplementary Information p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO Yuri Shibata, Masaaki Oyama, Hiroko Kozuka-Hata, Xiao Han, Yuetsu Tanaka,

More information

HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation

HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation SUPPLEMENTARY INFORMATION Materials and Methods Human cell lines and culture conditions HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation in exon 20 of BRCA1

More information

Supplementary Information

Supplementary Information Supplementary Information Supplementary Figure 1. CD4 + T cell activation and lack of apoptosis after crosslinking with anti-cd3 + anti-cd28 + anti-cd160. (a) Flow cytometry of anti-cd160 (5D.10A11) binding

More information

Impact factor: Reporter:4A1H0019 Chen Zi Hao 4A1H0023 Huang Wan ting 4A1H0039 Sue Yi Zhu 4A1H0070 Lin Guan cheng 4A1H0077 Chen Bo xuan

Impact factor: Reporter:4A1H0019 Chen Zi Hao 4A1H0023 Huang Wan ting 4A1H0039 Sue Yi Zhu 4A1H0070 Lin Guan cheng 4A1H0077 Chen Bo xuan Curcumin Protects Neonatal Rat Cardiomyocytes against High Glucose-Induced Apoptosis via PI3K/Akt Signalling Pathway Wei Yu,1,2 Wenliang Zha,1 Zhiqiang Ke,1 Qing Min,2 Cairong Li,1 Huirong Sun,3 and Chao

More information

(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)

(a) Significant biological processes (upper panel) and disease biomarkers (lower panel) Supplementary Figure 1. Functional enrichment analyses of secretomic proteins. (a) Significant biological processes (upper panel) and disease biomarkers (lower panel) 2 involved by hrab37-mediated secretory

More information

Plasma exposure levels from individual mice 4 hours post IP administration at the

Plasma exposure levels from individual mice 4 hours post IP administration at the Supplemental Figure Legends Figure S1. Plasma exposure levels of MKC-3946 in mice. Plasma exposure levels from individual mice 4 hours post IP administration at the indicated dose mg/kg. Data represent

More information

Introduction: 年 Fas signal-mediated apoptosis. PI3K/Akt

Introduction: 年 Fas signal-mediated apoptosis. PI3K/Akt Fas-ligand (CD95-L; Fas-L) Fas (CD95) Fas (apoptosis) 年 了 不 度 Fas Fas-L 力 不 Fas/Fas-L T IL-10Fas/Fas-L 不 年 Fas signal-mediated apoptosis 度降 不 不 力 U-118, HeLa, A549, Huh-7 MCF-7, HepG2. PI3K/Akt FasPI3K/Akt

More information

Supporting Information

Supporting Information Copyright WILEY-VCH Verlag GmbH & Co. KGaA, 69469 Weinheim, Germany, 212. Supporting Information for Adv. Funct. Mater., DOI:.2/adfm.2122233 MnO Nanocrystals: A Platform for Integration of MRI and Genuine

More information

Supplementary Information

Supplementary Information Supplementary Information Figure S1. Int6 gene silencing efficiency. (A) Western Blot analysis of Int6 expression at different times after sirna transfection. Int6 expression is strongly silenced in Int6

More information

Annals of Oncology Advance Access published January 10, 2005

Annals of Oncology Advance Access published January 10, 2005 Annals of Oncology Advance Access published January 10, 2005 Original article Annals of Oncology doi:10.1093/annonc/mdi077 Expression of survivin and bax/bcl-2 in peroxisome proliferator activated receptor-g

More information

BIK BIM NOXA PUMA MCL-1. p53

BIK BIM NOXA PUMA MCL-1. p53 HT116 cells A IK IM NOXA PUMA ML-1 p53 48 24 48 24 48 24 48 24 48 24 48 24 48 24 48 24 48 Procaspase 3 PARP leaved Product 12 8 4 24 hr 48 hr Figure S1. HT116 cell death by different proteasome inhibitors.

More information

C-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein. pigment isolated from Spirulina platensis. This water- soluble protein pigment is

C-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein. pigment isolated from Spirulina platensis. This water- soluble protein pigment is ' ^Summary C-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein pigment isolated from Spirulina platensis. This water- soluble protein pigment is of greater importance because of its various

More information

Effect of FADD expression during UVC carcinogenesis

Effect of FADD expression during UVC carcinogenesis Effect of FADD expression during UVC carcinogenesis Maja Radnić * *, Lidija Kalinić, Igor Matić, Ana Ruso, Inga Marijanović, Ivana Furčić and Biserka Nagy University of Zagreb, Faculty of Science, Department

More information

Effects of metallothionein-3 and metallothionein-1e gene transfection on proliferation, cell cycle, and apoptosis of esophageal cancer cells

Effects of metallothionein-3 and metallothionein-1e gene transfection on proliferation, cell cycle, and apoptosis of esophageal cancer cells Effects of metallothionein-3 and metallothionein-1e gene transfection on proliferation, cell cycle, and apoptosis of esophageal cancer cells Z.Q. Tian 1, Y.Z. Xu 1, Y.F. Zhang 1, G.F. Ma 1, M. He 1 and

More information

Anti-Apoptotic Effects of Cellular Therapy

Anti-Apoptotic Effects of Cellular Therapy Anti-Apoptotic Effects of Cellular Therapy Jason Lapetoda 1, Lee K Landeen, PhD 1, George K Michalopoulos, MD 2, Patricia W Bedard, PhD 1 1 Vital Therapies, Inc., San Diego, CA, USA 2 University of Pittsburgh

More information

Supplementary Figure S I: Effects of D4F on body weight and serum lipids in apoe -/- mice.

Supplementary Figure S I: Effects of D4F on body weight and serum lipids in apoe -/- mice. Supplementary Figures: Supplementary Figure S I: Effects of D4F on body weight and serum lipids in apoe -/- mice. Male apoe -/- mice were fed a high-fat diet for 8 weeks, and given PBS (model group) or

More information

Supplementary Figure 1

Supplementary Figure 1 A B D Relative TAp73 mrna p73 Supplementary Figure 1 25 2 15 1 5 p63 _-tub. MDA-468 HCC1143 HCC38 SUM149 MDA-468 HCC1143 HCC38 SUM149 HCC-1937 MDA-MB-468 ΔNp63_ TAp73_ TAp73β E C Relative ΔNp63 mrna TAp73

More information

RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using

RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using Supplementary Information Materials and Methods RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using Trizol reagent (Invitrogen,Carlsbad, CA) according to the manufacturer's instructions.

More information

Supplementary Materials and Methods

Supplementary Materials and Methods Supplementary Materials and Methods Immunoblotting Immunoblot analysis was performed as described previously (1). Due to high-molecular weight of MUC4 (~ 950 kda) and MUC1 (~ 250 kda) proteins, electrophoresis

More information

Supplementary Figures

Supplementary Figures Supplementary Figures Figure S1. Validation of kinase regulators of ONC201 sensitivity. Validation and screen results for changes in cell viability associated with the combination of ONC201 treatment (1

More information

General Laboratory methods Plasma analysis: Gene Expression Analysis: Immunoblot analysis: Immunohistochemistry:

General Laboratory methods Plasma analysis: Gene Expression Analysis: Immunoblot analysis: Immunohistochemistry: General Laboratory methods Plasma analysis: Plasma insulin (Mercodia, Sweden), leptin (duoset, R&D Systems Europe, Abingdon, United Kingdom), IL-6, TNFα and adiponectin levels (Quantikine kits, R&D Systems

More information

Page 39 of 44. 8h LTA & AT h PepG & AT h LTA

Page 39 of 44. 8h LTA & AT h PepG & AT h LTA Page 39 of 44 Fig. S1 A: B: C: D: 8h LTA 8h LTA & AT7519 E: F: 8h PepG G: 8h PepG & AT7519 Fig. S1. AT7519 overrides the survival effects of lipoteichoic acid (LTA) and peptidoglycan (PepG). (A) Human

More information

Samali A Figure S1.

Samali A  Figure S1. Deegan S, Saveljeva S, Logue SE, Pakos-Zebrucka K, Gupta S, Vandenabeele P, Bertrand MJ,Samali A. (2014) Deficiency in the mitochondrial apoptotic pathway reveals the toxic potential of autophagy under

More information

Supporting Information

Supporting Information Supporting Information Identification of Novel ROS Inducers: Quinone Derivatives Tethered to Long Hydrocarbon Chains Yeonsun Hong,, Sandip Sengupta,, Wooyoung Hur, *, Taebo Sim,, * KU-KIST Graduate School

More information

Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma

Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma Supplementary information for: Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma Rintaro Hashizume 1, Noemi Andor 2, Yuichiro Ihara 2, Robin Lerner 2, Haiyun

More information

The Biochemistry of apoptosis

The Biochemistry of apoptosis The Biochemistry of apoptosis 1 1 The apoptosis is composed of multiple biochemical events 2 2 Biochemical, cellular, and molecular events in Apoptosis 1. Membrane blebbing; phosphatidyl serine exposure

More information

An excessive increase in glutamate contributes to glucose-toxicity in. β-cells via activation of pancreatic NMDA receptors in rodent diabetes

An excessive increase in glutamate contributes to glucose-toxicity in. β-cells via activation of pancreatic NMDA receptors in rodent diabetes An excessive increase in glutamate contributes to glucose-toxicity in β-cells via activation of pancreatic NMDA receptors in rodent diabetes Xiao-Ting Huang 1, Chen Li 1,3, Xiang-Ping Peng 1, Jia Guo 1,5,

More information

SHREE ET AL, SUPPLEMENTAL MATERIALS. (A) Workflow for tumor cell line derivation and orthotopic implantation.

SHREE ET AL, SUPPLEMENTAL MATERIALS. (A) Workflow for tumor cell line derivation and orthotopic implantation. SHREE ET AL, SUPPLEMENTAL MATERIALS SUPPLEMENTAL FIGURE AND TABLE LEGENDS Supplemental Figure 1. Derivation and characterization of TS1-TGL and TS2-TGL PyMT cell lines and development of an orthotopic

More information

The Annexin V Apoptosis Assay

The Annexin V Apoptosis Assay The Annexin V Apoptosis Assay Development of the Annexin V Apoptosis Assay: 1990 Andree at al. found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium dependent manner.

More information

The Research of Nanocrystallized Realgar for the Treatment of Skin Cancer

The Research of Nanocrystallized Realgar for the Treatment of Skin Cancer Journal of Cancer Therapy, 2013, 4, 43-47 http://dx.doi.org/10.4236/jct.2013.46a1007 Published Online July 2013 (http://www.scirp.org/journal/jct) 43 The Research of Nanocrystallized Realgar for the Treatment

More information

Advances in Computer Science Research, volume 59 7th International Conference on Education, Management, Computer and Medicine (EMCM 2016)

Advances in Computer Science Research, volume 59 7th International Conference on Education, Management, Computer and Medicine (EMCM 2016) 7th International Conference on Education, Management, Computer and Medicine (EMCM 2016) Expression of Beta-Adrenergic Receptor in Glioma LN229 Cells and Its Effect on Cell Proliferation Ping Wang1, Qingluan

More information

For the rapid, sensitive and accurate measurement of apoptosis in various samples.

For the rapid, sensitive and accurate measurement of apoptosis in various samples. ab14082 500X Annexin V-FITC Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples. This product is for research use only and

More information

Supplementary Information. Induction of p53-independent apoptosis by ectopic expression of HOXA5

Supplementary Information. Induction of p53-independent apoptosis by ectopic expression of HOXA5 Supplementary Information Induction of p53-independent apoptosis by ectopic expression of in human liposarcomas Dhong Hyun Lee 1, *, Charles Forscher 1, Dolores Di Vizio 2, 3, and H. Phillip Koeffler 1,

More information

Supplementary Figure 1 P53 is degraded following Chlamydia infection independent of the cell lysis and protein sample preparation procedure applied.

Supplementary Figure 1 P53 is degraded following Chlamydia infection independent of the cell lysis and protein sample preparation procedure applied. Supplementary Figure 1 P53 is degraded following Chlamydia infection independent of the cell lysis and protein sample preparation procedure applied. (a) Western blotting analysis showing degradation of

More information

Supplemental Information

Supplemental Information Supplemental Information Tobacco-specific Carcinogen Induces DNA Methyltransferases 1 Accumulation through AKT/GSK3β/βTrCP/hnRNP-U in Mice and Lung Cancer patients Ruo-Kai Lin, 1 Yi-Shuan Hsieh, 2 Pinpin

More information

Original Article Bufalin attenuates the proliferation of breast cancer MCF-7 cells in vitro and in vivo by inhibiting the PI3K/Akt pathway

Original Article Bufalin attenuates the proliferation of breast cancer MCF-7 cells in vitro and in vivo by inhibiting the PI3K/Akt pathway Int J Clin Exp Med 2016;9(6):10297-10303 www.ijcem.com /ISSN:1940-5901/IJCEM0022878 Original Article Bufalin attenuates the proliferation of breast cancer MCF-7 cells in vitro and in vivo by inhibiting

More information

PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells

PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells PUMA gene transfection can enhance the sensitivity of epirubicin-induced apoptosis of MCF-7 breast cancer cells C.-G. Sun 1 *, J. Zhuang 1 *, W.-J. Teng 1, Z. Wang 2 and S.-S. Du 3 1 Department of Oncology,

More information

Key words: apoptosis, beta-amyrin, cell cycle, liver cancer, tritepenoids

Key words: apoptosis, beta-amyrin, cell cycle, liver cancer, tritepenoids JBUON 2018; 23(4): 965-970 ISSN: 1107-0625, online ISSN: 2241-6293 www.jbuon.com E-mail: editorial_office@jbuon.com ORIGINAL ARTICLE Antitumor effects of beta-amyrin in Hep-G2 liver carcinoma cells are

More information

Cordycepin induces apoptosis in human neuroblastoma SK-N-BE(2)-C and melanoma SK-MEL-2 cells

Cordycepin induces apoptosis in human neuroblastoma SK-N-BE(2)-C and melanoma SK-MEL-2 cells Indian Journal of Biochemistry & Biophysics Vol. 49, April 2012, pp. 86-91 Cordycepin induces apoptosis in human neuroblastoma SK-N-BE(2)-C and melanoma SK-MEL-2 cells Ji-Sue Baik, Haw-Young Kwon, Kyoung-Sook

More information

RayBio Annexin V-FITC Apoptosis Detection Kit

RayBio Annexin V-FITC Apoptosis Detection Kit RayBio Annexin V-FITC Apoptosis Detection Kit User Manual Version 1.0 May 25, 2014 (Cat#: 68FT-AnnV-S) RayBiotech, Inc. We Provide You With Excellent Support And Service Tel:(Toll Free)1-888-494-8555 or

More information

Caspase-3 Assay Cat. No. 8228, 100 tests. Introduction

Caspase-3 Assay Cat. No. 8228, 100 tests. Introduction Introduction Caspase-3 Assay Cat. No. 8228, 100 tests Caspase-3 is a member of caspases that plays a key role in mediating apoptosis, or programmed cell death. Upon activation, it cleaves a variety of

More information

(A) PCR primers (arrows) designed to distinguish wild type (P1+P2), targeted (P1+P2) and excised (P1+P3)14-

(A) PCR primers (arrows) designed to distinguish wild type (P1+P2), targeted (P1+P2) and excised (P1+P3)14- 1 Supplemental Figure Legends Figure S1. Mammary tumors of ErbB2 KI mice with 14-3-3σ ablation have elevated ErbB2 transcript levels and cell proliferation (A) PCR primers (arrows) designed to distinguish

More information

Annexin V-FITC Apoptosis Detection Kit

Annexin V-FITC Apoptosis Detection Kit ab14085 Annexin V-FITC Apoptosis Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Apoptosis in living cells (adherent and suspension). View kit datasheet: www.abcam.com/ab14085

More information

Induction of Apoptosis by L-NMMA, via FKHRL1/ROCK Pathway in Human Gastric Cancer Cells

Induction of Apoptosis by L-NMMA, via FKHRL1/ROCK Pathway in Human Gastric Cancer Cells BIOMEDICAL AND ENVIRONMENTAL SCIENCES 19, 285-291 (2006) Induction of Apoptosis by L-NMMA, via FKHRL1/ROCK Pathway in Human Gastric Cancer Cells YONG-ZHONG WANG AND ZHEN-QING FENG Department of Pathology,

More information

Islet viability assay and Glucose Stimulated Insulin Secretion assay RT-PCR and Western Blot

Islet viability assay and Glucose Stimulated Insulin Secretion assay RT-PCR and Western Blot Islet viability assay and Glucose Stimulated Insulin Secretion assay Islet cell viability was determined by colorimetric (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay using CellTiter

More information

Supplementary Materials for

Supplementary Materials for www.sciencesignaling.org/cgi/content/full/2/1/ra81/dc1 Supplementary Materials for Delivery of MicroRNA-126 by Apoptotic Bodies Induces CXCL12- Dependent Vascular Protection Alma Zernecke,* Kiril Bidzhekov,

More information

The subcortical maternal complex controls symmetric division of mouse zygotes by

The subcortical maternal complex controls symmetric division of mouse zygotes by The subcortical maternal complex controls symmetric division of mouse zygotes by regulating F-actin dynamics Xing-Jiang Yu 1,2, Zhaohong Yi 1, Zheng Gao 1,2, Dan-dan Qin 1,2, Yanhua Zhai 1, Xue Chen 1,

More information

Kaempferol inhibits gastric cancer tumor growth: An in vitro and in vivo study

Kaempferol inhibits gastric cancer tumor growth: An in vitro and in vivo study 868 Kaempferol inhibits gastric cancer tumor growth: An in vitro and in vivo study Haibin Song 1,2, Junjie BAO 1, Yuzhe Wei 1, Yang Chen 2, Xiaoguang Mao 2, Jianguo Li 2, Zhiwei Yang 2 and Yingwei Xue

More information

Supplementary Figure (OH) 22 nanoparticles did not affect cell viability and apoposis. MDA-MB-231, MCF-7, MCF-10A and BT549 cells were

Supplementary Figure (OH) 22 nanoparticles did not affect cell viability and apoposis. MDA-MB-231, MCF-7, MCF-10A and BT549 cells were Supplementary Figure 1. Gd@C 82 (OH) 22 nanoparticles did not affect cell viability and apoposis. MDA-MB-231, MCF-7, MCF-10A and BT549 cells were treated with PBS, Gd@C 82 (OH) 22, C 60 (OH) 22 or GdCl

More information

MTC-TT and TPC-1 cell lines were cultured in RPMI medium (Gibco, Breda, The Netherlands)

MTC-TT and TPC-1 cell lines were cultured in RPMI medium (Gibco, Breda, The Netherlands) Supplemental data Materials and Methods Cell culture MTC-TT and TPC-1 cell lines were cultured in RPMI medium (Gibco, Breda, The Netherlands) supplemented with 15% or 10% (for TPC-1) fetal bovine serum

More information

Silibinin Causes Apoptosis and Cell Cycle Arrest in Some Human Pancreatic Cancer Cells

Silibinin Causes Apoptosis and Cell Cycle Arrest in Some Human Pancreatic Cancer Cells Int. J. Mol. Sci. 2011, 12, 4861-4871; doi:10.3390/ijms12084861 OPEN ACCESS Article International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Silibinin Causes Apoptosis and Cell

More information

Supplementary Figures

Supplementary Figures Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein

More information

Apoptosis Oncogenes. Srbová Martina

Apoptosis Oncogenes. Srbová Martina Apoptosis Oncogenes Srbová Martina Cell Cycle Control point Cyclin B Cdk1 Cyclin D Cdk4 Cdk6 Cyclin A Cdk2 Cyclin E Cdk2 Cyclin-dependent kinase (Cdk) have to bind a cyclin to become active Regulation

More information

Receptor-interacting Protein Kinases Mediate Necroptosis In Neural Tissue Damage After Spinal Cord Injury

Receptor-interacting Protein Kinases Mediate Necroptosis In Neural Tissue Damage After Spinal Cord Injury Receptor-interacting Protein Kinases Mediate Necroptosis In Neural Tissue Damage After Spinal Cord Injury Haruo Kanno, M.D., Ph.D., Hiroshi Ozawa, M.D., Ph.D., Satoshi Tateda, M.D., Kenichiro Yahata, M.D.,

More information

Supporting Information. FADD regulates NF-кB activation and promotes ubiquitination of cflip L to induce. apoptosis

Supporting Information. FADD regulates NF-кB activation and promotes ubiquitination of cflip L to induce. apoptosis 1 2 Supporting Information 3 4 5 FADD regulates NF-кB activation and promotes ubiquitination of cflip L to induce apoptosis 6 7 Kishu Ranjan and Chandramani Pathak* 8 9 Department of Cell Biology, School

More information

University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; e Boone Pickens

University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; e Boone Pickens Nanosomes carrying doxorubicin exhibit potent anticancer activity against human lung cancer cells Akhil Srivastava a,h,+, Narsireddy Amreddy a,h,+, Anish Babu a,h, Janani Panneerselvam a,h, Meghna Mehta

More information

Marine Streptomyces sp. derived antimycin analogues. suppress HeLa cells via depletion HPV E6/E7 mediated by

Marine Streptomyces sp. derived antimycin analogues. suppress HeLa cells via depletion HPV E6/E7 mediated by Marine Streptomyces sp. derived antimycin analogues suppress HeLa cells via depletion HPV E6/E7 mediated by ROS-dependent ubiquitin proteasome system Weiyi Zhang 1, +, Qian Che 1, 2, +, Hongsheng Tan 1,

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Figure S1 Induction of non-apoptotic death of SV40-transformed and primary DKO MEFs, and DKO thymocytes. (A-F) STS-induced non-apoptotic death of DKO MEF. (A, B) Reduced viability of DKO MEFs after exposure

More information

SUPPLEMENTARY INFORMATION. Supplementary Figures S1-S9. Supplementary Methods

SUPPLEMENTARY INFORMATION. Supplementary Figures S1-S9. Supplementary Methods SUPPLEMENTARY INFORMATION SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membrane Jian Huang 1,2#, Jie Yan 1,2#, Jian Zhang 3#, Shiguo Zhu 1, Yanli Wang

More information

- 1 - Cell types Monocytes THP-1 cells Macrophages. LPS Treatment time (Hour) IL-6 level (pg/ml)

- 1 - Cell types Monocytes THP-1 cells Macrophages. LPS Treatment time (Hour) IL-6 level (pg/ml) Supplementary Table ST1: The dynamic effect of LPS on IL-6 production in monocytes and THP-1 cells after GdA treatment. Monocytes, THP-1 cells and macrophages (5x10 5 ) were incubated with 10 μg/ml of

More information

Chemical Chaperones Mitigate Experimental Asthma By Attenuating Endoplasmic

Chemical Chaperones Mitigate Experimental Asthma By Attenuating Endoplasmic Chemical Chaperones Mitigate Experimental Asthma By Attenuating Endoplasmic Reticulum Stress Lokesh Makhija, BE, Veda Krishnan, MSc, Rakhshinda Rehman, MTech, Samarpana Chakraborty, MSc, Shuvadeep Maity,

More information

MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells

MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells Margaret S Ebert, Joel R Neilson & Phillip A Sharp Supplementary figures and text: Supplementary Figure 1. Effect of sponges on

More information

Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the

Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the genome-wide methylation microarray data. Mean ± s.d.; Student

More information

CAN Resubmission SUPPLEMENTAL INFORMATION

CAN Resubmission SUPPLEMENTAL INFORMATION SUPPLEMENTAL INFORMATION CAN-05-0686 Resubmission Protein Immunoblotting. Primary antibodies used in this study include: Casp-9, Casp-3 and Survivin from Novus Biologicals (Littleton, CO); Casp-8 (Ab-3)

More information

RESEARCH COMMUNICATION. sirna Mediated Silencing of NIN1/RPN12 Binding Protein 1 Homolog Inhibits Proliferation and Growth of Breast Cancer Cells

RESEARCH COMMUNICATION. sirna Mediated Silencing of NIN1/RPN12 Binding Protein 1 Homolog Inhibits Proliferation and Growth of Breast Cancer Cells RESEARCH COMMUNICATION sirna Mediated Silencing of NIN1/RPN12 Binding Protein 1 Homolog Inhibits Proliferation and Growth of Breast Cancer Cells Wei-Yi Huang, Dong-Hui Chen, Li Ning, Li-Wei Wang* Abstract

More information

Supplemental Materials. STK16 regulates actin dynamics to control Golgi organization and cell cycle

Supplemental Materials. STK16 regulates actin dynamics to control Golgi organization and cell cycle Supplemental Materials STK16 regulates actin dynamics to control Golgi organization and cell cycle Juanjuan Liu 1,2,3, Xingxing Yang 1,3, Binhua Li 1, Junjun Wang 1,2, Wenchao Wang 1, Jing Liu 1, Qingsong

More information

Linderalactone inhibits human lung cancer growth b

Linderalactone inhibits human lung cancer growth b JBUON 2019; 24(2): 566-571 ISSN: 1107-0625, online ISSN: 2241-6293 www.jbuon.com E-mail: editorial_office@jbuon.com ORIGINAL ARTICLE Linderalactone inhibits human lung cancer growth by modulating the expression

More information

Annexin V-APC/7-AAD Apoptosis Kit

Annexin V-APC/7-AAD Apoptosis Kit Annexin V-APC/7-AAD Apoptosis Kit Catalog Number KA3808 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4

More information

Supplemental Table 1. Primers used for RT-PCR analysis of inflammatory cytokines Gene Primer Sequence

Supplemental Table 1. Primers used for RT-PCR analysis of inflammatory cytokines Gene Primer Sequence Supplemental Table 1. Primers used for RT-PCR analysis of inflammatory cytokines Gene Primer Sequence IL-1α Forward primer 5 -CAAGATGGCCAAAGTTCGTGAC-3' Reverse primer 5 -GTCTCATGAAGTGAGCCATAGC-3 IL-1β

More information

Figure S1. PMVs from THP-1 cells expose phosphatidylserine and carry actin. A) Flow

Figure S1. PMVs from THP-1 cells expose phosphatidylserine and carry actin. A) Flow SUPPLEMENTARY DATA Supplementary Figure Legends Figure S1. PMVs from THP-1 cells expose phosphatidylserine and carry actin. A) Flow cytometry analysis of PMVs labelled with annexin-v-pe (Guava technologies)

More information

Index 255. B lymphocytes, 26 Bak, 96 basal condition preparation, See also apoptosis measurement

Index 255. B lymphocytes, 26 Bak, 96 basal condition preparation, See also apoptosis measurement Index accurate apoptosis measurement, 23. See also apoptosis measurement activated Bax staining by flow cytometry, 105 106. See also mitochondrial membrane potential active caspase-3, 15 active caspase-3

More information

Pro-apoptotic signalling through Toll-like receptor 3 involves TRIF-dependent

Pro-apoptotic signalling through Toll-like receptor 3 involves TRIF-dependent Pro-apoptotic signalling through Toll-like receptor 3 involves TRIF-dependent activation of caspase-8 and is under the control of inhibitor of apoptosis proteins in melanoma cells Arnim Weber, Zofia Kirejczyk,

More information

NSCs), broblast growth factor, bfgf) ( Peprotech ) ; NSCs

NSCs), broblast growth factor, bfgf) ( Peprotech ) ; NSCs Chinese Journal of Pathophysiology 2003,19 (3) :289-292 289 [ ] 1000-4718 (2003) 03-0289 - 04 3 1, 1, 2, 1, 1, 1, 1, 2 ( 1, 2, 510060) [ ] :( ESCs) (NSCs) : ESCs, m ller,nscs 7 d,, RT - PCR nestin glutaminase

More information

Supplementary Table 1. Characterization of HNSCC PDX models established at MSKCC

Supplementary Table 1. Characterization of HNSCC PDX models established at MSKCC Supplementary Table 1. Characterization of HNSCC PDX models established at MSKCC Supplementary Table 2. Drug content and loading efficiency estimated with F-NMR and UV- Vis Supplementary Table 3. Complete

More information

Plasmids Western blot analysis and immunostaining Flow Cytometry Cell surface biotinylation RNA isolation and cdna synthesis

Plasmids Western blot analysis and immunostaining Flow Cytometry Cell surface biotinylation RNA isolation and cdna synthesis Plasmids psuper-retro-s100a10 shrna1 was constructed by cloning the dsdna oligo 5 -GAT CCC CGT GGG CTT CCA GAG CTT CTT TCA AGA GAA GAA GCT CTG GAA GCC CAC TTT TTA-3 and 5 -AGC TTA AAA AGT GGG CTT CCA GAG

More information

Effects of AFP gene silencing on Survivin mrna expression inhibition in HepG2 cells

Effects of AFP gene silencing on Survivin mrna expression inhibition in HepG2 cells mrna expression inhibition in HepG2 cells Z.L. Fang 1, N. Fang 2, X.N. Han 3, G. Huang 2, X.J. Fu 2, G.S. Xie 2, N.R. Wang 2 and J.P. Xiong 1 1 Department of Medical Oncology, The First Affiliated Hospital

More information

Supplementary Figure 1. Expression of CUGBP1 in non-parenchymal liver cells treated with TGF-β

Supplementary Figure 1. Expression of CUGBP1 in non-parenchymal liver cells treated with TGF-β Supplementary Figures Supplementary Figure 1. Expression of CUGBP1 in non-parenchymal liver cells treated with TGF-β and LPS. Non-parenchymal liver cells were isolated and treated with or without TGF-β

More information

Chapter 7: Modes of Cell Death

Chapter 7: Modes of Cell Death Chapter 7: Modes of Cell Death 7.1. Background Cell death can follow one of two distinct pathways, apoptosis or necrosis, and can occur in response to severe stress conditions or after exposure to toxic

More information

RESEARCH ARTICLE. Comparative Evaluation of Silibinin Effects on Cell Cycling and Apoptosis in Human Breast Cancer MCF-7 and T47D Cell Lines

RESEARCH ARTICLE. Comparative Evaluation of Silibinin Effects on Cell Cycling and Apoptosis in Human Breast Cancer MCF-7 and T47D Cell Lines RESEARCH ARTICLE Comparative Evaluation of Silibinin Effects on Cell Cycling and Apoptosis in Human Breast Cancer MCF-7 and T47D Cell Lines Zohreh Jahanafrooz 1, Nasrin Motameh 1 *, Behnaz Bakhshandeh

More information

Effective Targeting of Quiescent Chronic Myelogenous

Effective Targeting of Quiescent Chronic Myelogenous Cancer Cell, Volume 7 Supplemental Information Effective Targeting of Quiescent Chronic Myelogenous Leukemia Stem Cells by Histone Deacetylase Inhibitors in Combination with Imatinib Mesylate Bin Zhang,

More information

Therapeutic effect of baicalin on experimental autoimmune encephalomyelitis. is mediated by SOCS3 regulatory pathway

Therapeutic effect of baicalin on experimental autoimmune encephalomyelitis. is mediated by SOCS3 regulatory pathway Therapeutic effect of baicalin on experimental autoimmune encephalomyelitis is mediated by SOCS3 regulatory pathway Yuan Zhang 1,2, Xing Li 1,2, Bogoljub Ciric 1, Cun-gen Ma 3, Bruno Gran 4, Abdolmohamad

More information

microrna-200b and microrna-200c promote colorectal cancer cell proliferation via

microrna-200b and microrna-200c promote colorectal cancer cell proliferation via Supplementary Materials microrna-200b and microrna-200c promote colorectal cancer cell proliferation via targeting the reversion-inducing cysteine-rich protein with Kazal motifs Supplementary Table 1.

More information

Supplementary Figure 1. Validation of astrocytes. Primary astrocytes were

Supplementary Figure 1. Validation of astrocytes. Primary astrocytes were Supplementary Figure 1. Validation of astrocytes. Primary astrocytes were separated from the glial cultures using a mild trypsinization protocol. Anti-glial fibrillary acidic protein (GFAP) immunofluorescent

More information

Sarah A. Kopecky and Douglas S. Lyles*

Sarah A. Kopecky and Douglas S. Lyles* JOURNAL OF VIROLOGY, Apr. 2003, p. 4658 4669 Vol. 77, No. 8 0022-538X/03/$08.00 0 DOI: 10.1128/JVI.77.8.4658 4669.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved. Contrasting

More information

Understanding the mechanisms of asbestos related diseases

Understanding the mechanisms of asbestos related diseases University of Hawai i Cancer Center Understanding the mechanisms of asbestos related diseases Haining Yang, PhD Professor University of Hawai i Cancer Center Marker of exposure: Bilateral pleural plaques

More information

Supplementary figure legends

Supplementary figure legends Supplementary figure legends Supplementary Figure 1. Exposure of CRT occurs independently from the apoptosisassociated loss of the mitochondrial membrane potential (MMP). (A) HeLa cells treated with MTX

More information

Supplementary Figure 1. Dynamic Response of WT and mir-21 -/- mice to caerulein. (a) Representative histological sections of mouse pancreas stained

Supplementary Figure 1. Dynamic Response of WT and mir-21 -/- mice to caerulein. (a) Representative histological sections of mouse pancreas stained Supplementary Figure 1. Dynamic Response of WT and mir-21 -/- mice to caerulein. (a) Representative histological sections of mouse pancreas stained with hematoxylin from caerulein-treated WT and mir-21

More information

Supplementary Figure 1 Role of Raf-1 in TLR2-Dectin-1-mediated cytokine expression

Supplementary Figure 1 Role of Raf-1 in TLR2-Dectin-1-mediated cytokine expression Supplementary Figure 1 Supplementary Figure 1 Role of Raf-1 in TLR2-Dectin-1-mediated cytokine expression. Quantitative real-time PCR of indicated mrnas in DCs stimulated with TLR2-Dectin-1 agonist zymosan

More information

Effect of Survivin-siRNA on Drug Sensitivity of Osteosarcoma Cell Line MG-63

Effect of Survivin-siRNA on Drug Sensitivity of Osteosarcoma Cell Line MG-63 68 Chin J Cancer Res 22(1):68-72, 2010 www.springerlink.com Original Article Effect of Survivin-siRNA on Drug Sensitivity of Osteosarcoma Cell Line MG-63 Jing-Wei Wang 1, Yi Liu 2, Hai-mei Tian 2, Wei

More information

Essential Medium, containing 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin. Huvec were cultured in

Essential Medium, containing 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin. Huvec were cultured in Supplemental data Methods Cell culture media formulations A-431 and U-87 MG cells were maintained in Dulbecco s Modified Eagle s Medium. FaDu cells were cultured in Eagle's Minimum Essential Medium, containing

More information

MiRNA-202 impacts proliferation and apoptosis of granulose cells

MiRNA-202 impacts proliferation and apoptosis of granulose cells MiRNA-202 impacts proliferation and apoptosis of granulose cells Liqiong Huang, Bo Zheng*, Yi He Department of Obstetrics and Gynecology, Xianning Central Hospital, The First Affiliated Hospital Of Hubei

More information

Nature Immunology: doi: /ni Supplementary Figure 1. Production of cytokines and chemokines after vaginal HSV-2 infection.

Nature Immunology: doi: /ni Supplementary Figure 1. Production of cytokines and chemokines after vaginal HSV-2 infection. Supplementary Figure 1 Production of cytokines and chemokines after vaginal HSV-2 infection. C57BL/6 mice were (a) treated intravaginally with 20 µl of PBS or infected with 6.7x10 4 pfu of HSV-2 in the

More information

Construction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation

Construction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation Construction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation J. Du 1, Z.H. Tao 2, J. Li 2, Y.K. Liu 3 and L. Gan 2 1 Department of Chemistry,

More information

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 36: , 2015

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 36: , 2015 1464 Pre-stimulation of CD81 expression by resting B cells increases proliferation following EBV infection, but the overexpression of CD81 induces the apoptosis of EBV-transformed B cells GA BIN PARK 1*,

More information