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6 References 1. Biggs WS, Dery WH. Evaluation and treatment of constipation in infants and children. Am Fam Physician 006; 3(3): Amiel J, Sproat-Emison E, Garcia-Barcelo M, Lantieri F, Burzynski G, Borrego S, et al. Hirschsprung disease, associated syndromes and genetics: a review. J Med Genet 00; 5(1): de Lorijn F, Boeckxstaens GE, Benninga MA. Symptomatology, pathophysiology, diagnostic work-up, and treatment of Hirschsprung disease in infancy and childhood. Curr Gastroenterol Rep 00; 9(3): de Lorijn F, Kremer LC, Reitsma JB, Benninga MA. Diagnostic tests in Hirschsprung disease: a systematic review. J Pediatr Gastroenterol Nutr 006; (5): de Lorijn F, Reitsma JB, Voskuijl WP, Aronson DC, Ten Kate FJ, Smets AM, et al. Diagnosis of Hirschsprung's disease: a prospective, comparative accuracy study of common tests. J Pediatr 005; (6): De La Torre L, Langer JC. Transanal endorectal pull-through for Hirschsprung disease: technique, controversies, pearls, pitfalls, and an organized approach to the management of postoperative obstructive symptoms. Semin Pediatr Surg 010; 19(): Friedmacher F, Puri P. Residual aganglionosis after pull-through operation for Hirschsprung's disease: a systematic review and meta-analysis. Pediatr Surg Int 011; (10): Yang S, Donner LR. Detection of ganglion cells in the colonic plexuses by immunostaining for neuron-specific marker NeuN: an aid for the diagnosis of Hirschsprung disease. Appl Immunohistochem Mol Morphol 00; 10(3): Nakao M, Suita S, Taguchi T, Hirose R, Shima Y. Fourteen-year experience of acetylcholinesterase staining for rectal mucosal biopsy in neonatal Hirschsprung's disease. J Pediatr Surg 001; 36(9): Staines WA, Bettolli M, De CC, Swinton E, Sweeney B, Krantis A, et al. Fast evaluation of intraoperative biopsies for ganglia in Hirschsprung's disease. J Pediatr Surg 00; (1): Martucciello G. Hirschsprung's disease, one of the most difficult diagnoses in pediatric surgery: a review of the problems from clinical practice to the bench. Eur J Pediatr Surg 00; 1(3): "# $%! /5 / 31

7 Journal of Isfahan Medical School Received: Vol. 31, No. 5, th Week, November 013 Accepted: Number of Ganglion Cells in Human Colon Wall via Two Different Methods of Full-Thickness Sections and Fragmented Tissues Abstract Ardeshir Talebi MD 1, Mohammad Saeid Original Article Background: The most important way to diagnose Hirschsprung's disease is biopsy of rectum and its hystopathologic study by several slices (to 50 slices); detection of the disease depends on ganglion cells. Recently, there are two methods for detection of these cells; full-thickness sections and fragmented tissues. This study aimed to compare these to methods in detecting ganglion cells; which was not done before. Methods: This cross-sectional study done in Alzahra hospital (Isfahan, Iran) during , 5 samples of colon wall in patients with detected carcinoma were studied. A 1-cm slice from each sample was fixed in formalin. Another 1-cm slice from each sample was crushed and fixed on filter paper in formalin. The samples were prepared by tissue processor machine and colored by Hematoxylin and Eosin method. Both groups were coded by second person and analyzed by a unique pathologist. The number of ganglion cells was determined and recorded. Findings: The mean ± SD of counted ganglion cells was ± 11.0 in full-thickness sections and 5.6 ±.90 in fragmented tissues methods. According to independent sample t-test, the difference between two methods was statistically meaningful (P = 0.09). Conclusion: According to current study, fragmented tissues method is better than the full-thickness sections method in detection of ganglion cells for diagnosis of Hirschsprung's disease. Keywords: Ganglion cell, Full-thickness sections, Fragmented tissues, Hirschsprung's disease Citation: Talebi A, Saeid M. Number of Ganglion Cells in Human Colon Wall via Two Different Methods of Full-Thickness Sections and Fragmented Tissues. J Isfahan Med Sch 013; 31(5): 15- * This paper is derived from a medical doctorate thesis in Isfahan University of Medical Sciences. 1- Associate Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran - Student of Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Corresponding Author: Mohammad Saeid, mohamed003@yahoo.com 139 "# $%! /5 / 31 15