Infectious Disease Testing. UriSelect 4 Medium. Direct Identification Visibly Reliable

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1 Infectious Disease Testing Urielect 4 Medium Direct Identification Visibly Reliable

2 Urielect 4 Non selective chromogenic medium for the isolation, differentiation and enumeration of urinary tract infections pathogens within hours: Isolation and identification of both Gram-positive and Gram-negative pathogens in a single plate Chromogenic mix allowing the detection of specific enzymes activities Direct identification of E. coli, Enterococcus spp. and Proteus mirabilis via revelation and visualization of enzymatic activity of bacteria Presumptive identification of KEC, treptococci and taphylococci (diffentiation of. saprophyticus) Inhibition of Proteus spp. swarming with an original formulation Assay Procedure E. coli 24 hrs Proteus mirabilis 24 hrs Urine specimen inoculation with a 10 μl calibrated loop Incubation hrs at C Enumeration: Compare the density of colonies present on the plates, with those illustrated below: 10 6 CFU/mL 10 5 CFU/mL 10 4 CFU/mL 10 3 CFU/mL Interpretation of bacteriuria: Counting of well-isolated colonies on Urielect 4 should be possible up to 10 4 CFU/mL of urine. Above that concentration, presence of confluent colonies does not result in accurate counts.

3 Direct Identification of E. coli, Proteus mirabilis and Enterococcus spp. Escherichia coli Enterococcus spp. Pink colonies: β-galactosidase Indole Turquoise-blue colonies: β-glucosidase Proteus mirabilis Orange-brown colonies with possible brownish halo: TDA, Indole Presumptive Identification of KEC Klebsiella pneumoniae erratia marcescens Enterobacter cloacae Citrobacter koseri

4 Examples of Other Pathogens Growth taphylococcus saprophyticus taphylococcus aureus taphylococcus epidermidis treptococcus agalactiae Pseudomonas aeruginosa Candida albicans Acinetobacter baumanii

5 Pathogens Identification Observation of the color of colonies: Pink colonies = β-galactosidase Turquoise blue colonies = β-glucosidase Orange-brown colonies with a possible brownish halo = TDA ** Blue-purple colonies = β-galactosidase and β-glucosidase White, other color or colorless colonies Test for indole directly on a colony E.coli Examine under microscope Gram Cocci Enterococci Test for indole directly on a colony Proteus mirabilis Proteus spp., Morganella spp., Providencia spp. Examine under microscope Gram Bacilli very likely KEC * * Klebsiella, Enterobacter, erratia and Citrobacter ** Tryptophan Desaminase Complete the identification using a conventional procedure Visualization and Identification of Mixed Cultures Contrasting colors of colonies enhanced by a pearl-grey opaque background allow for an optimal visualization of polymicrobial mixtures. 18 hrs mixed culture of Klebsiella spp. and Enterococcus spp. 18 hrs mixed culture of E. coli, Enterococcus spp. and Proteus spp.

6 Ordering Information Catalog # Description Urinary Tract Infections Urielect 4, 20 plates x 90 mm Urielect 4, 100 plates x 90 mm Urielect 4 (dehydrated), 500 g Kovacs reagent, 2 vials x 15 ml Urea Indole medium, 50 ampules x 1 ml O.X. (Oxidase), 2 x 50 disks Novobiocin (5 µg), 30 disks Pastorex taph Plus, 50 tests Pastorex taph Plus, 5 x 50 tests Pastorex trep, 60 tests AuxaColor 2 kit, 20 tests Visit for more information. Catalog # Description Rapid Detection of Resistance to Third Generation Cephalosporins for Enterobacteriaceae b LACTA Test, 50 tests Rapid Detection of Carbapenemase-Producing Enterobacteriaceae b CARBA Test, 25 tests Rapid Identification of Carbapenemases Types K-15R1 OXA-48 K-eT* K-15R2 KPC K-eT* * Manufactured by and distributed by Bio-Rad. Ask for availability in your country. Other Chromogenic Media MRAelect II, 20 plates x 90 mm Aelect, 20 plates x 90 mm VREelect, 20 plates x 90 mm trepbelect, 20 plates x 90 mm Candielect, 20 plates x 90 mm Aelect VREelect MRAelect II Urielect 4 trepbelect Candielect Chromogenic Media Range Bio-Rad Laboratories Clinical Diagnostics Group Website Australia Austria Belgium +32 (3) Brazil +55 (31) Canada China Czech Republic Denmark Finland France Germany +49 (0) Greece Hong Kong Hungary India Israel Italy Japan Korea Mexico +52 (55) The Netherlands New Zealand Norway Poland Portugal Russia ingapore outh Africa pain weden witzerland +41 (0) /06 Taiwan Thailand United Kingdom +44 (0) Bulletin Rev D Printed in France with soy-based inks on paper from sustainably managed forests Bio-Rad 2016/02