Stimulating the immune system to fight persistent parasites and microorganisms that infect the red blood cells Raúl Morales M.D.
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1 Stimulating the immune system to fight persistent parasites and microorganisms that infect the red blood cells Raúl Morales M.D.
2 This review Describes the experience with the development of a novel form of immunotherapy that represents the first practical and effective means of performing antigent-loaded dendritic cell immunotherapy, against infections in red blood cells.
3 Stimulating the immune system to fight parasites and microorganisms that infect the red blood cells like: Viruses, bacterias, yeasts and other foreign invaders. Borrellia Babesia Bartonella
4 Dendritic Cells Pulsed with infected red blood cells, peptide components, induce anti-infection immunity.
5 Extracorporeal photopheresis with ultraviolet light theraphy (UV-B, UV-A and C.) Mononuclear and disinfected red cells are obtained by extracorporeal photopheresis, then incubated with IL-4 and GM-CSF. (colony stimulating factors).
6 The electromagnetic spectrum of ultraviolet radiation Name Abbreviation Wavelength range (nanometers) Energy per photon (electron-volts) Notes / alternative names Ultraviolet A UBA nm ev long wave, black light, not absorbed by the ozone layer Ultraviolet B UVB nm ev medium wave, mostly absorbed by the ozone layer Ultraviolet C UVC nm ev short wave, germicidal, completely absorbed by the ozone layer
7 The method Upon exposure to UVA light, covalent crosslinking of RNA or DNA of the cells occurs. This ultimately results in the proliferative arrest of the treated infected cells. UVA light wavelength
8 Mononuclear cells (dendritic cells) are pulsed with the patient's own infected red cells, using fragmented peptides, to induce anti-infection immunity. This biological product will be returned to the patient as a vaccine against infected red cells.
9 The photodynamic therapy, inducing cell maturation. Mononuclear Cell Mature and educated Dendritic Cell The passage of the cells through UV light will convert them to mature and educated dendritic cells. (DCs)
10 How this works: Upon exposure to UVA light, covalent crosslinking of DNA occurs. This results in the proliferative arrest of the treated cells and programs cell death (apoptosis). photosensitizer agents UV Light DNA sequence Apoptotic cell
11 Stimulating the immune system T-cell mediated immune response The generation of an effective T cellmediated immune response requires the activation of both the cytotoxic (CD8+) and the helper (CD4+) T lymphocyte subsets.
12 Stimulating the immune system Injection of growth factors, combined with antigens in vivo, will attract autologous DCs to the immunization site where they will uptake the injected antigens.
13 Stimulating the immune system : In light of these findings, the combination of extracorporeal photopheresis, red cells exchange, and immunizations with autologous dendritic cells cultured ex vivo, propelled with infected red blood cells antigens, is a wonderful strategy to elicit a strong immune response in these patients.
14 Biomedical Center s Founder Raul Morales, M.D., founded the Institute in 1981; Dr. Morales, Associate Professor of Hematology, is a graduate of Medicine of the University Of Guadalajara, Mexico. He received his post-graduate training, Internal Medicine and sub-specialized in Hematology and transfusional medicine at the National University of Mexico. He is a member of the Mexican Society of Hematology, American Society of Hematology, American Society of Clinical Oncology, International Society of Hematology, Association European of Hematology, American Association of Blood Banks, and the National Mexican Council of Hematology. Dr. Morales is an active academic representative of the University of Guadalajara. He has the responsibility of establishing relationships among universities and other medical institutions with the purpose of exchanging knowledge and experiences in scientific and academic affairs.
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