MIC Test Strip for antimicrobial susceptibility testing

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Britton Carroll
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for antimicrobial susceptibility testing Liofilchem Liofilchem Quantitative assay for determining the Minimum Inhibitory Co

are porous strips with special features (International Patent) that are impregnated with a predefined concentration gradient of antibiotic, across 15

For ESBL, MBL, GRD, AmpC and KPC detection, the double-sided gradient carries the appropriate diagnostic reagents.

Mixed culture on Chromatic MH Meropenem 0.002-32 μg/ml Staphylococcus aureus ATCC 29213 Tigecycline 0.016-256 μg/ml M.I.C. = 0.

transferred to the agar matrix. After 18 hours incubation or longer, a symmetrical inhibition ellipse centered along the strip is formed.

Other growth/inhibition patterns may also be seen for resistance detection methods.

Performance Standards for Antimicrobial Susceptibility Testing. CLSI M7-A10, 2015.

CLSI M11-A8 Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria.

1 for antimicrobial susceptibility testing Liofilchem Liofilchem Quantitative assay for determining the Minimum Inhibitory Concentration (M.I.C.) is a quantitative assay for determining the Minimum Inhibitory Concentration (M.I.C.) of antimicrobial agents against microorganisms and for detecting the resistance mechanisms. are porous strips with special features (International Patent) that are impregnated with a predefined concentration gradient of antibiotic, across 15 two-fold dilutions of a conventional M.I.C. method. On one side of the strip is indicated a M.I.C. scale in μg/ml and a code that identify the antimicrobial agent. For ESBL, MBL, GRD, AmpC and KPC detection, the double-sided gradient carries the appropriate diagnostic reagents. are available in a large variety of configurations. Each configuration is available in packages of 10, 30 and 100 tests. Mixed culture on Chromatic MH Meropenem μg/ml Staphylococcus aureus ATCC Tigecycline μg/ml M.I.C. = 0.19 μg/ml method principle When the is applied onto an inoculated agar surface, the preformed exponential gradient of antimicrobial agent is immediately transferred to the agar matrix. After 18 hours incubation or longer, a symmetrical inhibition ellipse centered along the strip is formed. The MIC is read directly from the scale in terms of μg/ml at the point where the edge of the inhibition ellipse intersects the strip. Other growth/inhibition patterns may also be seen for resistance detection methods. Staphylococcus aureus Vancomycin / Teicoplanin GRD+ Klebsiella pneumoniae Ertapenem / Ert. +Phenylboronic acid KPC + BIBLIOGRAPHY CLSI M100-S27, Performance Standards for Antimicrobial Susceptibility Testing. CLSI M7-A10, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically. CLSI M11-A8 Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. CLSI M11-S1 Performance Standards for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. CLSI M27-A3. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard - Third Edition. CLSI M27-S4. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Fourth Informational supplement. EUCAST. Breakpoint tables Please for interpretation contact info@ewcdiagnostics.com of MICs and zone or call diameters +31 (0) Version 7.1, March 2017.

for ANTIMICROBIAL susceptibility testing AMIKACIN 0.016-256 AK 920181 92018 920180 AMOXICILLIN 0.

2 for ANTIMICROBIAL susceptibility testing AMIKACIN AK AMOXICILLIN AML AMOXICILLIN*-CLAVULANIC ACID (2/1) CLSI recommended AMOXICILLIN*-CLAVULANIC ACID (2 μg/ml) EUCAST recommended * AUG * AMC AMPICILLIN AMP AMPICILLIN*-SULBACTAM (2/1) CLSI recommended AMPICILLIN*-SULBACTAM (4 μg/ml) EUCAST recommended * AMS * SAM AZITHROMYCIN AZM AZTREONAM ATM AZTREONAM ATM BACITRACIN BA CEFACLOR CEC CEFAZOLIN KZ CEFEPIME FEP CEFIXIME CFM CEFOPERAZONE*-SULBACTAM (2/1) * CPS CEFOTAXIME CTX CEFOTAXIME CTX CEFOTETAN CTT CEFOXITIN FOX CEFPIROME CR CEFPODOXIME PX CEFTAROLINE CPT CEFTAROLINE CPT CEFTAZIDIME (FDA cleared) CAZ CEFTAZIDIME*-AVIBACTAM * CZA CEFTIBUTEN CTB CEFTIZOXIME CZX CEFTOBIPROLE BPR CEFTOLOZANE*-TAZOBACTAM (FDA cleared) * C/T

for ANTIMICROBIAL susceptibility testing CEFTRIAXONE 0.016-256 CRO 920421 92042 920420 CEFTRIAXONE 0.002-32 CRO CEFUROXIME 0.016-256 CXM CEPHALOTHIN 0.016-256 KF CHLORAMPHENICOL 0.

3 for ANTIMICROBIAL susceptibility testing CEFTRIAXONE CRO CEFTRIAXONE CRO CEFUROXIME CXM CEPHALOTHIN KF CHLORAMPHENICOL C CIPROFLOXACIN CIP CLARITHROMYCIN CLR CLINDAMYCIN (FDA cleared) CD CLOXACILLIN CX Sensitest COLISTIN SENSITEST NEW! CS 16 tests Sensitest SENSITEST COLISTIN NEW! CS 16 tests DALBAVANCIN (FDA cleared) DAL DAPTOMYCIN (Includes Ca 2+ ) DAP DELAFLOXACIN (FDA cleared) DLX DORIPENEM DOR DOXYCYCLINE DXT ENROFLOXACIN ENR ERTAPENEM ETP ERYTHROMYCIN E FLORFENICOL FFC FOSFOMYCIN (Includes Glucose-6-Phosphate) FOS FOSFOMYCIN (Includes Glucose-6-Phosphate) FOS FUSIDIC ACID FU GATIFLOXACIN GAT GEMIFLOXACIN GEM GENTAMICIN CN GENTAMICIN CN IMIPENEM IMI KANAMYCIN K LEVOFLOXACIN LEV

for ANTIMICROBIAL susceptibility testing LINEZOLID 0.016-256 LNZ 921351 92135 921350 MARBOFLOXACIN 0.002-32 MAR MECILLINAM 0.016-256 MEC MEROPENEM (FDA cleared) 0.002-32 MRP MEROPENEM 0.

4 for ANTIMICROBIAL susceptibility testing LINEZOLID LNZ MARBOFLOXACIN MAR MECILLINAM MEC MEROPENEM (FDA cleared) MRP MEROPENEM MRP METRONIDAZOLE MTZ MOXIFLOXACIN MXF MUPIROCIN MUP MINOCYCLINE MN NALIDIXIC ACID NA NETILMICIN NET NORFLOXACIN NOR NITROFURANTOIN F OFLOXACIN OFX OXACILLIN OX PENICILLIN G P PENICILLIN G P PIPERACILLIN PIP PIPERACILLIN*-TAZOBACTAM (4 μg/ml) * TZP POLYMYXIN B PB QUINUPRISTIN-DALFOPRISTIN QDA RIFAMPICIN RD RIFAMPICIN RD SPECTINOMYCIN SPC SPIRAMYCIN SP STREPTOMYCIN S SULBACTAM SUL SULFAMETHOXAZOLE SMX TEDIZOLID (FDA cleared) TZD TEICOPLANIN TEC

for ANTIMICROBIAL susceptibility testing TELAVANCIN 0.002-32 TLV 920521 92052 920520 TELAVANCIN (FDA cleared) 0.016-256 TLV TEMOCILLIN 0.064-1024 TMO TETRACYCLINE 0.016-256 TE TIAMULIN 0.

5 for ANTIMICROBIAL susceptibility testing TELAVANCIN TLV TELAVANCIN (FDA cleared) TLV TEMOCILLIN TMO TETRACYCLINE TE TIAMULIN TIA TICARCILLIN*-CLAVULANIC ACID (2 μg/ml) * TTC TIGECYCLINE TGC TILMICOSIN TIL TOBRAMYCIN TOB TOBRAMYCIN TOB TRIMETHOPRIM TM TRIMETHOPRIM*-SULFAMETHOXAZOLE (1/19) * SXT VANCOMYCIN (FDA cleared) VA for ANTIFUNGAL susceptibility testing AMPHOTERICIN B AMB ANIDULAFUNGIN AND CASPOFUNGIN CAS FLUCONAZOLE FLU FLUCYTOSINE FC ISAVUCONAZOLE IVU ITRACONAZOLE ITC KETOCONAZOLE KE MICAFUNGIN MYC POSACONAZOLE POS VORICONAZOLE VO for ANTIMYCOBACTERIAL susceptibility testing ETHAMBUTOL EB ETHIONAMIDE ET ISONIAZIDE IZ

ESBL for confirmation of Extended Spectrum Beta-Lactamase CEFEPIME/CEFEPIME+CLAVULANIC ACID (4 μg/ml) 0.25-16 / 0.064-4 FEP/FEL 921611 92161 921610 CEFOTAXIME/CEFOTAXIME+CLAVULANIC ACID (4 μg/ml) 0.

064-4 CAZ/CAL for KPC detection 921601 92160 921600 921591 92159 921590 921671 MEROPENEM / MEROPENEM + PHENYLBORONIC ACID 0.125-8 / 0.

6 ESBL for confirmation of Extended Spectrum Beta-Lactamase CEFEPIME/CEFEPIME+CLAVULANIC ACID (4 μg/ml) / FEP/FEL CEFOTAXIME/CEFOTAXIME+CLAVULANIC ACID (4 μg/ml) / CTX/CTL CEFTAZIDIME/CEFTAZIDIME+CLAVULANIC ACID (4 μg/ml) / CAZ/CAL for KPC detection MEROPENEM / MEROPENEM + PHENYLBORONIC ACID / MRP/MBO ERTAPENEM / ERTAPENEM + PHENYLBORONIC ACID / ETP/EBO MBL for detection of Metallo Beta-Lactamase IMIPENEM / IMIPENEM + EDTA / 1-64 IMI/IMD IMIPENEM / IMIPENEM + EDTA / IMI/IMD MEROPENEM / MEROPENEM + EDTA / MRP/MRD GRD for detection of Glycopeptide Resistance VANCOMYCIN / TEICOPLANIN / VA/TEC for AmpC detection CEFOTETAN / CEFOTETAN+CLOXACILLIN / CTT/CXT ERTAPENEM / ERTAPENEM + CLOXACILLIN / ETP/ECX MTS Synergy Application System (International Patent) Description Packaging Ref. MTS Synergy Applicator Platform 1 unit MTS Synergy Delivery Tool 10 disposable applicators Accessories Description Packaging Ref. DEN-1B McFarland densitometer with built-in adapter for 18 mm tubes A-16 Adapter for 16 mm tubes CKG18 calibration kit for DEN-1, for 18mm glass tubes CKG16 calibration kit for DEN-1, for 16mm glass tubes Storage Tube with Desiccant 10 tubes McFarland Standard Set tubes McFarland 1 tube McFarland 1 tube McFarland 1 tube

Ready to use culture media DESCRIPTION FORMAT PACKAGING REF.

recommended: for antimicrobial susceptibility testing of Streptococcus spp.

MUELLER HINTON AGAR II + 2% NaCl for antimicrobial susceptibility testing

CHROMATIC MH Chromogenic medium for the preliminary identification and

susceptibility testing of anaerobic bacteria.

for Haemophilus spp. susceptibility testing.

10031 10231 10131 11231 10132 11132 11206 11618 10246 10245 11065 10080

094 μg/ml S. pneumoniae ATCC 49619 CRO 0.016-256 μg/ml M.I.C. = 0.

pneumoniae ATCC 49619 E 0.016-256 μg/ml M.I.C. = 0.032 μg/ml C.

7 Ready to use culture media DESCRIPTION FORMAT PACKAGING REF. MUELLER HINTON AGAR II for antimicrobial susceptibility testing of non-fastidious organisms. MUELLER HINTON AGAR II + SHEEP BLOOD 5% for antimicrobial susceptibility testing of fastidious organisms. MUELLER HINTON FASTIDIOUS AGAR (Horse blood 5% + 20 mg/l β-nad) EUCAST recommended: for antimicrobial susceptibility testing of Streptococcus spp., Haemophilus spp., and some other fastidious organisms. MUELLER HINTON AGAR II + 2% NaCl for antimicrobial susceptibility testing of staphylococci with Oxacillin. CHROMATIC MH Chromogenic medium for the preliminary identification and susceptibility testing of bacteria directly from clinical and environmental specimens. BRUCELLA BLOOD AGAR with HEMIN AND VITAMIN K1 for antimicrobial susceptibility testing of anaerobic bacteria. SCHADLER K AGAR (SHEEP BLOOD 5%) for antimicrobial susceptibility testing of anaerobic bacteria. HAEMOPHILUS TEST AGAR for Haemophilus spp. susceptibility testing. MIDDLEBROOK 7H11 AGAR for antimicrobial susceptibility testing of mycobacteria. R.P.M.I. AGAR (2% glucose + MOPS) for antifungal susceptibility testing E. coli ATCC SXT μg/ml M.I.C. = μg/ml S. pneumoniae ATCC CRO μg/ml M.I.C. = μg/ml S. aureus ATCC FOX μg/ml M.I.C. = 1.5 μg/ml S. pneumoniae ATCC E μg/ml M.I.C. = μg/ml C. krusei ATCC 6258 POS μg/ml M.I.C. = μg/ml S. aureus ATCC FU μg/ml M.I.C. = 0.50 μg/ml C. krusei VO μg/ml M.I.C. = 0.25 μg/ml P. aeruginosa ATCC TZP μg/ml M.I.C. = 8 μg/ml S. maltophilia ATCC IMI/IMD 4-256/1-64 μg/ml MBL+ E. coli DSM CTX/CTL / μg/ml ESBL+ E. coli CAZ/CAL / μg/ml ESBL+ K. pneumoniae ATCC BAA-1144 CTT/CXT / μg/ml AmpC+

Discussion points CLSI M100 S19 Update. #1 format of tables has changed. #2 non susceptible category

Discussion points CLSI M100 S19 Update. #1 format of tables has changed. #2 non susceptible category Discussion points 2009 CLSI M100 S19 Update Nebraska Public Health Laboratory Changes most important to routine antimicrobial susceptibility testing. Documents available Janet Hindler discussion slide