Oral Fluid Drug Testing. Vincent Di Fazio, Sarah Wille, Nele Samyn
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1 Oral Fluid Drug Testing Vincent Di Fazio, Sarah Wille, Nele Samyn
2 Objectives Pros and Cons of OF as Toxicological Matrix OF Screening Devices : What to expect? Practical considerations for OF analysis QC issues Can OF be used in Toxicology?
3 Introduction OF as toxicological matrix Passive diffusion Pysicochemical properties of drug Salivary flow / ph Trapping of basic drugs Contamination after smoking or sniffing
4 Introduction OF as toxicological matrix Easy collection (no medical trained personel) OF better linked to window of impairment then urine Screening DOA No link with impairment Detection of (recent) drug use
5 Introduction Link Blood/OF? Wille et al., Ther. Drug Monit. (2009) 31 p 511
6 OF Screening devices ROSITA devices Biomedica Oralstat Branan medical Oratect Cozart RapiScan Dräger DrugTest Lifepoint Impact Securetec Drugwipe Sun Biomedical Oraline Ultimed SalivaScreen Varian Oralab Lots of failed tests >25% (OF matrix) Low THC sensitivity: 13-75%
7 OF Screening devices ROSITA
8 OF Screening devices DRUID devices Biosensor Biosens Dynamic Branan medical Oratect III Cozart DDS Dräger DrugTest 5000 Mavand RapidSTAT Securetec Drugwipe 5 + Innovacon OrAlert Varian Oralab 6 THC sensitivity is an issue
9 OF Screening devices DRUID
10 OF Screening devices DRUID
11 OF Screening devices 2010
12 OF Screening devices Desrosiers et al., Clin Chem(2012) 58 p 1418
13 OF Screening devices Implementation: Belgian DUID legislation
14 OF Screening devices Implementation: Belgian DUID legislation
15 OF Analysis / Collection Collection approaches Collection device versus spitting, drooling (non)stimulated collection Impact on: Saliva flow Stability Recovery Drug concentration
16 OF Analysis / Collection Collection approaches Ramaekers et al., Drug Alc Depend (2006) 85 p 114 Huestis and Cone, J Anal Toxicol (2004) 28 p 394
17 OF Analysis / Collection Collection approaches: stability and recovery Langel et al., J Anal Toxicol (2008) 32 p 393
18 OF Analysis / Collection Collection approaches and recovery Wille et al., Therapeutic Drug Monit (2013) 35 p 101
19 OF Analysis / Practical considerations Collected OF Mix OF / Buffer
20 OF Analysis / Practical considerations Collected OF Wille et al., Therapeutic Drug Monit (2013) 35 p 101 THC concentration in neat oral fluid is calculated using the following formula, with the presumption that 1 ml of neat oral fluid weighs 1 g: C THC = C UPLC/MS x (V buffer + w-y) dilutionfactor x (w-y) C UPLC/MS : THC concentration calculated using the calibration curve C THC : THC concentration in neat oral fluid V buffer : buffer volume in collector device W : weight collector after oral fluid collection Y : mean weight of empty collector ( g: StatSure collector; g: Quantisal collector; g: Concateno collector) Dilutionfactor : volume of buffer + theoretical collected neat oral fluid volume
21 OF Analysis / Practical considerations Collected OF Volume (g) C OF (ng/g) C neat OF (ng/g) Coc MDMA THC Coc MDMA THC X
22 OF Analysis / Analysis Factors to consider for chromatografic analysis Dilution Matrix effects Matrix effect as % recovery (CV%) C THC Statsure Quantisal Certus LOW 77 (5) 45 (7) 111 (13) HIGH 75 (10) 56 (14) 102 (9) Matrix effect IS as % recovery (CV%) C THC Statsure Quantisal Certus LOW 99 (3) 88 (3) 100 (3) HIGH 99 (7) 94 (9) 102 (4)
23 OF Analysis / Interpretation Concentration? Moore et al., Forensic Sci Int (2011) 212 p 227
24 OF Analysis / Interpretation Concentration? Toennes SW et al., J Anal Toxicol (2010) 34 p 216
25 OF Analysis / Quality Control ISO QC and PT Concentration (ng/ml) n spiked labresult CV% THC Coc BE MDMA MDA Amf Mef Morph MAM Cod
26 Conclusion / Discussion Pros and Cons of OF as Toxicological Matrix? Sample collection on-site No link with impairment Detection of (recent drug use) OF Screening devices : What to expect? Easy on-site use THC sensitivity increase Practical considerations OF collection and influence on Drug Concentration QC issues? PT schemes tackeling THC stability are necessary
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