PerkinElmer talks at spring TCJJP Meeting Wednesday, Mar. 14, 2012

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1 Courtney Phillips Wednesday, March 14,

2 In 1996, the FQPA amended the two federal laws governing pesticides Federal Insecticide, Fungicide FIFRA& Rodenticide Act Federal Food, Drugs & FDCA Cosmetics Act 2

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5 SPE Preparation Quechers Dilution Automated variants Separation Liquid Chromatography* Detection MS/MS High Resolution Mass Analyzer Preparation SPE Quechers Solid Phase Extraction Separates analytes of interest Dilution from a complex matrix Phases similar to LC columns Requires Automated vacuum manifold variants ~ 5 10 min/sample Separation Liquid Chromatography* Detection MS/MS High Resolution Mass Analyzer courtney.phillips@perkinelmer.com 5

6 Preparation Separation SPE Quechers Dilution Quick, Easy, Cheap, Effective, Rugged & Safe Developed specifically for Automated pesticides variants analysis in foods Created as an alternative to the Luke method Requires centrifuge ~ 5 10 min/sample Liquid Chromatography* Detection MS/MS High Resolution Mass Analyzer Preparation SPE Quechers Dilution Automated variants Separation Detection Liquid Chromatography* Considerations for Liquid Chromatography Mass analyzers require lower flow rates UHPLCs have the ability to run at these flow rates However, MS/MStraditional UHPLC columns may not be ideal Consider Superficially Porous Particle (SPP) columns High Resolution Mass Analyzer courtney.phillips@perkinelmer.com 6

7 SPE Preparation Quechers Dilution Automated variants Triple Quadruple Instrument Separation Liquid Chromatography* Detection MS/MS High Resolution Mass Analyzer Preparation Separation SPE Quechers Dilution Automated variants Time of Flight (TOF) Instrument Liquid Chromatography* Detection MS/MS High Resolution Mass Analyzer 7

8 LC-TOF Pesticides by LC-TOF Acephate Oxamyl Methomyl Mevinphos isomers Dimethoate Acetamiprid Mevinphos Anilazine Malathion/metalochlor Prometryne/dichloran Spinosad A Diazinon courtney.phillips@perkinelmer.com 8

9 Pesticides by LC-TOF Pesticides by LC-TOF Linearity of Pesticides from 0.6ppb to 10,000ppb 9

10 Spiked Pesticides in Celery Extraction by Quechers RP-LC Pos ion mode Dual probe ESI- TOF with lock mass calibration Carbendazim in Orange Juice RP-HPLC Brownlee C18 SPP 10cm Gradient from 30% 100% MeOH Ambient temp, 0.3mL/min Flow m/z spectra/sec Drying Gas Temp 350 o C Dual ESI Source with Lock Mass Drying Gas Flow 12 L/min Capillary exit voltage = 90 V courtney.phillips@perkinelmer.com 10

11 Carbendazim in Orange Juice RP-HPLC Brownlee C18 SPP 10cm Gradient from 30% 100% MeOH Ambient temp, 0.3mL/min Flow m/z spectra/sec Drying Gas Temp 350 o C Dual ESI Source with Lock Mass Drying Gas Flow 12 L/min Capillary exit voltage = 90 V Carbendazim in Orange Juice RP-HPLC Brownlee C18 SPP 10cm Gradient from 30% 100% MeOH Ambient temp, 0.3mL/min Flow m/z spectra/sec Drying Gas Temp 350 o C Dual ESI Source with Lock Mass Drying Gas Flow 12 L/min Capillary exit voltage = 90 V courtney.phillips@perkinelmer.com 11

12 Carbendazim in Orange Juice All data, all the time 12

13 All data, all the time All data, all the time 13

14 LC-TOF A NEW TECHNOLOGY courtney.phillips@perkinelmer.com 14

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19 Pomegranate juice adulterated with 1% Grape Juice Considerations for sample preparation time, expense & desired results 19

20 Quantitation requires separation: Liquid Chromatography Detection requires a mass analyzer such as a triple quad or a time of flight instrument courtney.phillips@perkinelmer.com 20

21 Screening & Identification can be achieved with a DSA probe Consider the future: Will you need to know more? courtney.phillips@perkinelmer.com 21

22 Questions? 22