The Effects of Shampoo on Microbial Flora. Andrew Walker Grade 9 Central Catholic High School
|
|
- Kimberly Richardson
- 5 years ago
- Views:
Transcription
1 The Effects of Shampoo on Microbial Flora Andrew Walker Grade 9 Central Catholic High School
2 Shampoo Hair care product used to clean hair of unwanted build up Combined soap, water, and herbs to make hair shine and create a pleasant fragrance Liquid shampoo was invented in 1927, by German Hans Schwarzkopf Salt is used to adjust viscosity
3 Variable (V05 Shampoo) Contains: Vitamin A Vitamin H Vitamin C Vitamin B5 Vitamin B3
4 Gram (+) vs. Gram (-) Bacteria Most pathogenic bacteria in humans are Gram (+) organisms. Simple cell wall Some antibiotics work against the formation of the cell wall. Cell was contains an extra layer of lipopolysaccharides for extra protection. Outer membrane protects bacteria from several antibiotics.
5 Escherichia coli Rod-shaped, 2 micrometers diameter Gram negative bacteria Reproduction rate: 20 min Survival, growth, and replication require only a single carbon source and ammonium salts. Common symbiont found in the lower intestines of warm blooded animals. Few pathogenic - Almost 73,000 cases of infection and 61 deaths per year in the United States. Most common prokaryote model
6 Staphylococcus epidermidis Gram positive bacteria Common surface symbiont in many mammals (including humans) Most forms considered non-pathogenic. Potentially pathogenic upon systemic entry Forms biofilms
7 Rationale The human micro flora performs many functions vital to human health. Almost every person the world uses shampoo when they bathe. Does shampoo effect the micro flora on the human scalp?
8 Hypothesis Null Hypothesis- The shampoo will have no effect on the survivorship of E. coli and Staph Alternate Hypothesis- The shampoo will have an effect on the survivorship of E. coli and Staph
9 Materials Variable (V05 Shampoo) Micro pipettes + Sterile tips Spreader bars LB agar plates (1% Tryptone, 0.5% Yeast extract, 1% NaCl) Escherichia coli DH5- alpha(e.coli) (Obtained from Doonan Lab, CMU) Staphylococcus epidermidis (Staph) (Obtained from Ward s Scientific) Burner Sidearm Flask Vortex Incubator SDF (Sterile Dilution Fluid) Sterile Test Tubes Ethanol Klett spectrophotometer
10 Procedure Bacteria (E.coli and Staph) were grown overnight in sterile LB Media. Samples of the overnight cultures were added to fresh media in a sterile sidearm flask. The cultures were placed in an incubator (37 C) until a density of 50 Klett spectrophotometer units was reached. This represents a cell density of approximately 10⁸ cells/ml. The cultures were diluted in sterile dilution fluid to a concentration of approximately 10⁵ cells/ml. The experimental variables were mixed with the appropriate amounts of SDF to create concentrations of 0%,0.1%, 1%, and 10%.
11 Procedure (cont.) Concentration Chart 0% 0.1% 1% 10% 0.1 ml microbe 0.1 microbe 0.1 microbe 0.1 microbe 0 ml variable 0.01 ml variable 0.1 ml variable 1 ml variable 9.9 ml SDF 9.89 ml SDF 9.8 ml SDF 8.9 ml SDF
12 Procedure (cont.) 100 µl of cell culture was then added to shampoo solutions, yielding a final volume of 10 ml and a cell density of approximately 103 cells/ml. The solutions were vortexed and allowed to sit at room temperature for 15 minutes. After vortexing to evenly suspend the cells, 100 µl aliquots were removed from the tubes and spread on LB-agar plates. The plates were incubated at 37 C for 24 hours. The resulting colonies were counted visually. Each colony was assumed to have arisen from one cell.
13 ANOVA and Dunnett s Test ANOVA Statistical Test that allows the compares the means multiple groups. Dunnett s Test A follow-up test to an ANOVA, Comparison of an experimental and a control group to determine significant variation.
14 Number of Colonies E. Coli Averages P-value: E % 0.10% 1% 10% Number of Colonies Concentration of Shampoo
15 Dunnett s Statistical Analysis (E. coli) Shampoo Concentration T Value Significance 0.1% 5.32 Significant 1% 4.81 Significant 10% 8.96 Significant T-crit= 3.29
16 Number of Colonies 900 Survivorship Curve (E. coli) Number of Colonies % 0.10% 1% 10% Concentration of Shampoo
17 Number of Colonies S. epidermidis Averages P-value: E % 0.10% 1% 10% number of colonies Concentration of Shampoo
18 Dunnett s Statistical Analysis (Staph) Shampoo Concentration T Value Significance 0.1% Significant 1% Significant 10% Significant T-crit= 3.29
19 Number of Colonies 450 Survivorship Curve (Staph) number of colonies % 0.10% 1% 10% Concentration of Shampoo
20 LD50 LD50- Lethal Dose 50%, what concentration kills 50% of the colonies Type of Bacteria LD50 Escherichia coli 3% concentration Staphylococcus epidermidis 0.065% concentration
21 Conclusions The null hypothesis was rejected, and the alternate hypothesis was accepted for both E. coli and Staph. The shampoo (V05 Shampoo) did significantly reduce on the survivorship of E. coli and Staph.
22 Limitations Future Studies Plating slightly unsynchronized Only one kind of test, liquid pulse Only 4 concentrations of variable tested Only 1 exposure time Use more types of energy drinks. Use more concentrations and exposure times Agar infusion Synergistic effects
23 Thank you for your time and attention. Are there any questions?
24 ANOVA (E. coli) Anova: Single Factor SUMMARY Groups Count Sum Average Variance 0% % % % ANOVA Source of Variation SS df MS F P-value F crit Between Groups E Within Groups Total
25 ANOVA (staph) Anova: Single Factor SUMMARY Groups Count Sum Average Variance 0% % % % ANOVA Source of Variation SS df MS F P-value F crit Between Groups E Within Groups Total
Synergistic Effects of Vitamin B12 and Creatine on Microbes. Jake Rocchi CCHS, 11th grade 2nd year in PJAS
Synergistic Effects of Vitamin B12 and Creatine on Microbes Jake Rocchi CCHS, 11th grade 2nd year in PJAS Vitamin B12 Water-soluble vitamin Needed for proper functioning in brain, nervous system, and blood
More informationEffects of Ethyl Alcohol on Microbial Survivorship. Tim Olson 9th Grade Central Catholic High School
Effects of Ethyl Alcohol on Microbial Survivorship Tim Olson 9th Grade Central Catholic High School Ethyl Alcohol Pure alcohol or drinking alcohol Oldest recreational drug Alcohol intoxication Inhibits
More informationHydrogen Peroxide Influence on Microbial Survivorship. Jacob Cebulak Central Catholic Pittsburgh Grade 9
Hydrogen Peroxide Influence on Microbial Survivorship Jacob Cebulak Central Catholic Pittsburgh Grade 9 Problem Humans use excess hydrogen peroxide to clean wounds. The concentration used is often damaging
More informationAntimicrobial Effects of Vinegar. Daniel Crawford Grade 9 Central Catholic High School
Antimicrobial Effects of Vinegar Daniel Crawford Grade 9 Central Catholic High School Antibacterial Agents Humans interest in microbial growth/survivorship for over 100 years Many products/technologies
More informationBy Tony Patsy, Pittsburgh Central Catholic, Grade 9 ARTIFICIAL SWEETENER EFFECTS ON MICROBIAL FLORA
By Tony Patsy, Pittsburgh Central Catholic, Grade 9 ARTIFICIAL SWEETENER EFFECTS ON MICROBIAL FLORA PROBLEM Saccharin is consumed by many humans on a day to day basis. Overconsumption of saccharin may
More informationTHE EFFECTS OF SPORTS DRINKS ON E.COLI SURVIVORSHIP JONATHAN FARKOS GRADE 9 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL
THE EFFECTS OF SPORTS DRINKS ON E.COLI SURVIVORSHIP JONATHAN FARKOS GRADE 9 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL PROBLEM Do sports drinks have adverse effects on microflora cells? HUMAN SYMBIONTS, PATHOGENS,
More informationThe Effects of Alcohol and Nicotine on Microbial Flora. Jeff Van Kooten Grade 11 Pittsburgh Central Catholic High School
The Effects of Alcohol and Nicotine on Microbial Flora Jeff Van Kooten Grade 11 Pittsburgh Central Catholic High School Microbial Flora The internal and external flora has eukaryotic fungi, protists, and
More informationVitamin C and Ibuprofen Effects on Escherichia Coli. Timothy Leisenring Grade 11 Central Catholic High School
Vitamin C and Ibuprofen Effects on Escherichia Coli Timothy Leisenring Grade 11 Central Catholic High School Rationale for Experiment Commonly, ingested materials are investigated for effects on human
More informationBY ZACHARY MODISPACHER 11 TH GRADE CENTRAL CATHOLIC HIGH SCHOOL
BY ZACHARY MODISPACHER 11 TH GRADE CENTRAL CATHOLIC HIGH SCHOOL INTRODUCTION Chicken is one of the most consumed meats in the world, though can pose health risks (salmonella). Salmonella was thought only
More informationThe Effects of Reiki on Bacteria Survivorship. Jordan Ciccone Central Catholic High School
The Effects of Reiki on Bacteria Survivorship Jordan Ciccone Central Catholic High School Reiki Japanese spiritual healing practice meaning universal life energy. Training is done by a Reiki Master. Either
More informationArtificial Sweetener Effects on Microbial Flora. Thomas Peilert Central Catholic High School
Artificial Sweetener Effects on Microbial Flora Thomas Peilert Central Catholic High School Problem Can the artificial sweetener Equal Original affect survivorship of Escherichia coli? Microbial Flora
More informationChlorine and UV Effects on Bacteria Survivorship. Oscar Shaver Grade 9 Central Catholic High School
Chlorine and UV Effects on Bacteria Survivorship Oscar Shaver Grade 9 Central Catholic High School Electromagnetic Spectrum Diagram showing the range of all types of frequencies of electromagnetic radiation
More informationBlair Bean Grade 9 Pittsburgh Central Catholic Highschool
Blair Bean Grade 9 Pittsburgh Central Catholic Highschool Variety of organisms that live in and on the body Skin Gastrointestinal tracts Nose Pharynx Mouth Conjunctiva Provide moisture, nourishment, and
More informationBy: Stephen Pfau Pittsburgh Central Catholic High School Grade 9
By: Stephen Pfau Pittsburgh Central Catholic High School Grade 9 Everyday thousands of people take cold/flu medicine and pain relief medicine Two common medicines for cold/flu and pain are DayQuil (cold)
More informationNIKLAS BALDIS- CENTRAL CATHOLIC HIGH SCHOOL MUSIC INFLUENCE ON BACTERIAL REPLICATION
NIKLAS BALDIS- CENTRAL CATHOLIC HIGH SCHOOL MUSIC INFLUENCE ON BACTERIAL REPLICATION MUSIC Elements: pitch, rhythm, timbre, texture, and dynamics Different types of music can exclude and include certain
More informationEvaluation of Antibacterial Effect of Odor Eliminating Compounds
Evaluation of Antibacterial Effect of Odor Eliminating Compounds Yuan Zeng, Bingyu Li, Anwar Kalalah, Sang-Jin Suh, and S.S. Ditchkoff Summary Antibiotic activity of ten commercially available odor eliminating
More informationBill Boehner Pittsburgh Central Catholic PJAS 2014 Grade 11
Bill Boehner Pittsburgh Central Catholic PJAS 2014 Grade 11 The technical term for saltiness in aquatic environments is halinity, from the fact that halides, or chloride, are the most abundant anions in
More informationAmoeba Therapy on Streptococcus Pneumonia. By Sam Casale
Amoeba Therapy on Streptococcus Pneumonia By Sam Casale Microbial Therapy A lesser used form of therapy. Involves using microorganisms to fight other microorganisms. Phage Therapy is the most commonly
More informationRecipes for Media and Solution Preparation SC-ura/Glucose Agar Dishes (20mL/dish, enough for 8 clones)
Protocol: 300 ml Yeast culture preparation Equipment and Reagents needed: Autoclaved toothpicks Shaker Incubator set at 30 C Incubator set at 30 C 60 mm 2 sterile petri dishes Autoclaved glass test tubes
More informationResearch Article. The effects of hyaluronic acid on the morphological physiological differentiation of Lactobacillus
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(7):368-372 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 The effects of hyaluronic acid on the morphological
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com FORMULATION AND EVALUATION OF HERBAL WASH FOR NASAL HEALTH
More informationInternational Journal of Pharma and Bio Sciences A COMPARITIVE STUDY OF ANTIMICROBIAL ACTIVITY OF SOME HERBS AND THEIR SYNERGISTIC EFFECT ABSTRACT
Research Article Microbiology International Journal of Pharma and Bio Sciences ISSN 0975-6299 A COMPARITIVE STUDY OF ANTIMICROBIAL ACTIVITY OF SOME HERBS AND THEIR SYNERGISTIC EFFECT SNEHA GOGTE*¹ AND
More informationA.Kavitha Assistant professor Department of Botany RBVRR Womens college
A.Kavitha Assistant professor Department of Botany RBVRR Womens college The Ultrastructure Of A Typical Bacterial Cell The Bacterial Cell This is a diagram of a typical bacterial cell, displaying all of
More informationComparative study of the disinfection capacity of different floor cleaning solutions on ventilated room floor
Comparative study of the disinfection capacity of different floor cleaning solutions on ventilated room floor M. G. Sanal Kumar, S. Nandakumar, B. Bini & Arya Raj R. S. P.G. & Research Department of Zoology,
More informationHigher plants produced hundreds to thousands of diverse chemical compounds with different biological activities (Hamburger and Hostettmann, 1991).
4. ANTIMICROBIAL ACTIVITY OF PHYSALIS MINIMA L. 4.1. Introduction Use of herbal medicines in Asia represents a long history of human interactions with the environment. Plants used for traditional medicine
More informationEXPERIMENT. Bacteria Identification through Functional Media Motility Testing
EXPERIMENT Bacteria Identification through Functional Media Motility Testing Hands-On Labs, Inc. Version 42-0248-00-02 Review the safety materials and wear goggles when working with chemicals. Read the
More informationSupplementary Information. Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food
Supplementary Information Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative Lipsy Chopra, Gurdeep Singh, Kautilya Kumar Jena and Debendra K. Sahoo* Biochemical
More informationRadiation Inactivation of Microbes in Fresh Vegetables
Radiation Inactivation of Microbes in Fresh Vegetables ZENAIDA M. DE GUZMAN Supervising Science Research Specialist Philippine Nuclear Research Institute PHILIPPINE NUCLEAR RESEARCH INSTITUTE PNRI Co-60
More informationBiological Consulting Services
Biological Consulting Services of North Florida/ Inc. May 13, 2009 Aphex BioCleanse Systems, Inc. Dear Sirs, We have completed antimicrobial efficacy study on the supplied Multi-Purpose Solution. The testing
More informationInternational Journal of Food Nutrition and Safety, 2012, 1(2): International Journal of Food Nutrition and Safety
International Journal of Food Nutrition and Safety, 2012, 1(2): 54-59 International Journal of Food Nutrition and Safety Journal homepage: www.modernscientificpress.com/journals/ijfns.aspx ISSN: 2165-896X
More informationSurvival of Aerobic and Anaerobic Bacteria in
APPLIED MICROBIOLOGY, Mar. 1968, p. 445-449 Copyright 1968 American Society for Microbiology Vol. 16, No. 3 Printed in U.S.A. Survival of Aerobic and Anaerobic Bacteria in Chicken Meat During Freeze-Dehydration,
More informationMALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology
MALDI-TOF Mass Spectrometry: A New Rapid ID Method in Clinical Microbiology Patrick R. Murray, PhD WW Director, Scientific Affairs BD Diagnostic Systems Outline MALDI-TOF is the most important innovation
More informationFIT TECHNICAL DATA. Summary of Research Studies and Production Trials
FIT TECHNICAL DATA Summary of Research Studies and Production Trials Fit Efficacy vs. Bacteria Pathogens Bacteria Pathogen Staphylococcus Aureus (ATCC 8) Listeria Monocytogenes (ATCC 9) Escherichia Coli
More informationChapter 4. Anti-bacterial studies of PUFA extracts from Sardinella longiceps and Sardinella fimbriata. 4.1 Introduction
Anti-bacterial studies of PUFA extracts from Sardinella longiceps and Sardinella fimbriata C o n t e n t s 4.1 Introduction 4.2 Materials and Methods 4.2.1 Extract Preparation and Determination of PUFA
More informationBACTERIAL EXAMINATION OF WATER
BACTERIAL EXAMINATION OF WATER The bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing,
More informationProfessor Diane Hilker
Professor Diane Hilker I. Exp. 19: Water Analysis for Fecal Contamination II. Exp. 20: Quantitative Analysis of H 2 O Purpose: To learn & perform the 3 stage standard H 2 O analysis test for fecal contamination
More informationLESSON 2.6 WORKBOOK Diagnosing infections, and, what s up your nose?
Staphylococcus aureus Morphology: The physical form or structure of a microbe.. LESSON 2.6 WORKBOOK Diagnosing infections, and, what s up your nose? Now we have discussed the different requirements that
More informationThe Effects of Spray Sunscreen on MG63 Cancer Cell Lines. Anthony Williams Pittsburgh Central Catholic High School Grade 11
The Effects of Spray Sunscreen on MG63 Cancer Cell Lines Anthony Williams Pittsburgh Central Catholic High School Grade 11 Sunscreen Meant to protect against harmful UV rays that can be carcinogenic Comes
More informationScreening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal
Screening of bacteria producing amylase and its immobilization: a selective approach By Debasish Mondal Article Summary (In short - What is your article about Just 2 or 3 lines) Category: Bacillus sp produce
More informationTKB1 Competent Cells. Instruction Manual. Research Use Only. Not for Use in Diagnostic Procedures. Catalog # Revision B
TKB1 Competent Cells Instruction Manual Catalog #200134 Revision B Research Use Only. Not for Use in Diagnostic Procedures. 200134-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement
More informationEXPERIMENT. Food Safety
EXPERIMENT Food Safety Hands-On Labs, Inc. Version 42-0308 -00-01 Review the safety materials and wear goggles when working with chemicals. Read the entire exercise before you begin. Take time to organize
More informationEXPERIMENT. Bacteria Identification through Functional Media Motility Testing
EXPERIMENT Bacteria Identification through Functional Media Motility Testing Hands-On Labs, Inc. Version 42-0248-00-02 Review the safety materials and wear goggles when working with chemicals. Read the
More informationLab #9. Introduction. Class samples:
Lab #9 Introduction Food-borne illness is largely caused by the presence of bacteria in red meat. However, much of these harmful bacteria can be destroyed and prevented by sanitation and safe cooking practices.
More informationMicrobial Quality Analysis of Milk and Flavoured Milk Products from Local Vendors in Vellore
Microbial Quality Analysis of Milk and Flavoured Milk Products from Local Vendors in Vellore Aditya Sood*, Ridhi Sood, Abhijit Kumar, Gaganjot Kaur, Candy Sidhu Assistant Professor, Chandigarh University,
More informationDetermination of MIC & MBC
1 Determination of MIC & MBC Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight
More informationProphylactic effect of yogurt on Fusobacterium nucleatum in the mouth. Brigham Yang
Prophylactic effect of yogurt on Fusobacterium nucleatum in the mouth Brigham Yang BIOL 494 Mentor: Dr. R. Shane Gold 8 th April 2014 Abstract Periodontal diseases are closely related to the activity of
More informationASSESMENT OF CRYOPRESERVATION SYSTEMS INFLUENCE ON THE SURVAVIAL OF E. COLI RECOMBINANT STRAINS
Lucrări ştiinńifice Zootehnie şi Biotehnologii, vol. 41(1) (2008), Timişoara ASSESMENT OF CRYOPRESERVATION SYSTEMS INFLUENCE ON THE SURVAVIAL OF E. COLI RECOMBINANT STRAINS TESTAREA INFLUENłEI SISTEMELOR
More informationIn vitro study of antibacterial activity of Carissa carandas leaf extracts
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (1):36-40 ISSN : 2249-7412 CODEN (USA): AJPSKY In vitro study of antibacterial activity of Carissa
More informationCalibration Protocols
Calibration Protocols (1)OD 600 Reference point LUDOX Protocol Materials: 1ml LUDOX CL-X (provided in kit) ddh20 (provided by team) 96 well plate, black with clear flat bottom preferred (provided by team)
More information(44) is microconidiating, fluffy, inositolless, isoleucineless, and valineless.
THE EFFECT OF PHOTOREACTIVATION ON MUTATION FREQUENCY IN NEUROSPORA' JEANETTE SNYDER BROWN Stanford University, Stanford, California Received for publication April 1, 1951 Kelner (1949a) first reported
More informationBioprospecting of Neem for Antimicrobial Activity against Soil Microbes
ISSN: 2454-132X Impact factor: 4.295 (Volume3, Issue1) Available online at: www.ijariit.com Bioprospecting of Neem for Antimicrobial Activity against Soil Microbes R. Prasanna PRIST University, Tamilnadu
More informationTHE MOUTHWASH CHALLENGE
THE MOUTHWASH CHALLENGE Ginny Brown Churchill HS / Montgomery Co. MD Abstract A problem assignment to design a new mouthwash that will combat the growth of bacteria that is frequently blamed for gum disease.
More informationA Study of Consumer Storage of Commercially Available Peanut Butter with Analysis of Variance
A Study of Consumer Storage of Commercially Available Peanut Butter with Analysis of Variance Abstract Outbreaks of Salmonella contamination of peanut butter have become a serious food safety concern for
More informationSummary and Conclusion
Summary and Conclusion Milk and milk products are consumed daily by Indian citizens. This type of food is easily perishable and support growth of foodborne pathogens keeping this view in mind the investigation
More informationBACTERIAL GROWTH. FYBSc.
BACTERIAL GROWTH FYBSc. Bacterial growth Binary fission Generation time Phases of growth 4-2 Binary fission 1. Prokaryote cells grow by increasing in cell number (as opposed to increasing in size). 2.
More informationNormal Flora. CLS 212: Medical Microbiology
Normal Flora CLS 212: Medical Microbiology Relationships between Organisms Symbiosis Permanent association between two different organisms. Neutralism Two organisms living together, and neither is affected
More informationO. Repeat the measurement in all relevant modes used in your experiments (e.g. settings for orbital averaging).
Before You Begin Read through this entire protocol sheet carefully before you start your experiment and prepare any materials you may need. This year, in order to improve reproducibility, we are requiring
More informationOZONE EFFECTS ON SPECIFIC BACTERIA AND VIRUSES (
Bacterial infections and the spread of viruses relating to athletic gear are becoming a bigger concern each year. Numerous articles are being written and public awareness is growing rapidly to the health
More informationEvaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples
Evaluation of the feasibility of the VACUETTE Urine CCM tube for microbial testing of urine samples Background The VACUETTE Urine CCM tube is for the collection, transport and storage of urine samples
More informationMt. San Antonio College Microbiology 22 Lab Schedule for Spring 2018 Mon/Weds. Split Lab Sections ONLY
Mt. San Antonio College Microbiology 22 Lab Schedule for Spring 2018 Mon/ Split Lab Sections ONLY Wk 1 Feb. 26 Orientation with Introductions & Safety Rules/Regulations Feb. 28 Orientation with Pathogen
More informationMt. San Antonio College Microbiology 22 Lab Schedule for Spring 2018 Tues/Thurs. Split Lab Sections ONLY
Mt. San Antonio College Microbiology 22 Lab Schedule for Spring 2018 Tues/ Split Lab Sections ONLY Wk 1 Feb. 27 Orientation with Introductions & Safety Rules/Regulations March 1 Orientation with Pathogen
More informationStriatal Neuron Medium Kit
Striatal Neuron Medium Kit Product Information What are included in the Striatal Neuron Medium Kit (ax0333): 2x 250 ml Striatal Neuron Basal Medium (Store at 4 o C upon receipt) 2x 7.5 ml Striatal Neuron
More informationTHE TABACCO LEAF EXTRACT AND ANTIBACTERIAL ACTIVITY IN TEXTILE
THE TABACCO LEAF EXTRACT AND ANTIBACTERIAL ACTIVITY IN TEXTILE Pichet Duangsri 1, Kantima Juntarapun 2, Chutimon Satirapipathkul 3 * Chulalongkorn University, Faculty of Engineering, Department of Chemical
More informationAperto Cell Lysis and Protein Solubilization Users Manual
Aperto Cell Lysis and Protein Solubilization Users Manual Revision 2 THIS MANUAL APPLIES TO THE FOLLOWING PRODUCTS: 3A8600 Aperto, 5X Cell Lysis Buffer. 20mL 3A8610 Aperto, 5X Cell Lysis Buffer. 100mL
More informationPRESENTER: DENNIS NYACHAE MOSE KENYATTA UNIVERSITY
18/8/2016 SOURCES OF MICROBIAL CONTAMINANTS IN BIOSAFETY LABORATORIES IN KENYA PRESENTER: DENNIS NYACHAE MOSE KENYATTA UNIVERSITY 1 INTRODUCTION Contamination occurs through avoidable procedural errors
More informationInfluence of Different Prebiotics and Probiotics on Selective Intestinal Pathogens
ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 657-663 http://www.ijcmas.com Original Research Article Influence of Different Prebiotics and Probiotics on Selective Intestinal Pathogens Anayata Sharma 1*
More informationBACTERIAL PLATING INSTRUCTIONS AND INTERPRETATION. SHELLFISH BACTERIAL WORKSHOP APRIL 26, 2017 SUSAN LARAMORE
BACTERIAL PLATING INSTRUCTIONS AND INTERPRETATION SHELLFISH BACTERIAL WORKSHOP APRIL 26, 2017 SUSAN LARAMORE slaramo1@fau.edu 772 242 2525 Spread Plate Method This is the easiest and most common plating
More informationß-Galactosidase Repression in Escherichia coli B23 Using Minimal Concentrations of Glucose and Sucrose
ß-Galactosidase Repression in Escherichia coli B23 Using Minimal Concentrations of Glucose and Sucrose JILLIAN CLARK, JACQUIE HUDSON, ROBIN MAK, CHRISTA McPHERSON, AND CARMEN TSIN Department of Microbiology
More informationExercise 15-B PHYSIOLOGICAL CHARACTERISTICS OF BACTERIA CONTINUED: AMINO ACID DECARBOXYLATION, CITRATE UTILIZATION, COAGULASE & CAMP TESTS
Exercise 15-B PHYSIOLOGICAL CHARACTERISTICS OF BACTERIA CONTINUED: AMINO ACID DECARBOXYLATION, CITRATE UTILIZATION, COAGULASE & CAMP TESTS Decarboxylation of Amino Acids and Amine Production The decarboxylation
More informationPathogenicity of Infectious Diseases
Pathogenicity of Infectious Diseases Pathogenicity of Infectious Diseases HOST DISEASE TRIAD PATHOGEN ENVIRONMENT OTHER MICROBES Microbial Interactions KOCH'S POSTULATES Four criteria that were established
More informationBiology Multiple Choice, 2 pt each.
Biology 3340 Spring 2007 Name Exam 1, Version A Write your name on both the exam booklet and the mark sense sheet. On the upper left corner of the mark sense sheet in the Key ID box, mark the version letter
More informationScreening and isolation of microbial contaminants from carbonated and non-carbonated soft drinks of Delhi
International Journal of Emerging Trends in Science and Technology IC Value: 76.89 (Index Copernicus) Impact Factor: 4.219 DOI: https://dx.doi.org/10.18535/ijetst/v4i5.04 Screening and isolation of microbial
More informationMt. San Antonio College Microbiology 22 Lab Schedule for Fall 2017 Tues/Thurs. Split Lab Sections ONLY
Mt. San Antonio College Microbiology 22 Lab Schedule for Fall 2017 Tues/ Split Lab Sections ONLY Wk 1 Aug. 29 Orientation with Introductions & Safety Rules/Regulations Aug. 31 Orientation with Pathogen
More informationCHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS
CHAPTER 8 ANTIBACTERIAL ACTIVITY OF THE CRUDE ETHANOLIC EXTRACT AND THE ISOLATED COMPOUNDS FROM THE STEM OF COSTUS IGNEUS 8.1 INTRODUCTION Medicinal plants are the backbone of traditional medicine and
More informationIn-vitro analysis of the microbial-load in raw meat and finished products
ISSN: 2319-7706 Volume 3 Number 12 (2014) pp. 643-648 http://www.ijcmas.com Original Research Article In-vitro analysis of the microbial-load in raw meat and finished products M. P.Prasad* Department of
More informationIntroduction to Microbiology The Microbial World and You (Chapter 1) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College
Introduction to Microbiology The Microbial World and You (Chapter 1) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Primary Source for figures and content: Eastern Campus
More informationSOP History Number Date Reason for Change v1 10/10/2014 Original V2 10/10/2016 Update V3 10/10/2018 Update
Document Number: SASoM/METHOD/073.v3 Title: Version: Author: Lentivirus production from HEK293T cells v3 Paul Reynolds Effective from: 10/10/2018 Valid to: 09/10/2020 SOP History Number Date Reason for
More informationComparison of a Waterless, Scrubless CHG/Ethanol Surgical Scrub to Traditional CHG and Povidone-Iodine Surgical Scrubs
Comparison of a Waterless, Scrubless CHG/Ethanol Surgical Scrub to Traditional CHG and Povidone-Iodine Surgical Scrubs by M. Sigler 1 J. Bastyr 2 J. Stahl 2 J. Pyrek 2 1 Stephens and Associates, Inc.,
More informationIndigenous fermented milk products: A microbiological study in Bhagalpur town
RESEARCH ARTICLE FOOD SCIENCE RESEARCH JOURNAL Volume 4 Issue 1 April, 2013 24-28 Indigenous fermented milk products: A microbiological study in Bhagalpur town A.R. NIGAM, R.P. SAH AND MD. IRSHAD ALAM
More informationAdenovirus Manual 1. Table of Contents. Large Scale Prep 2. Quick MOI Test 4. Infection of MNT-1 Cells 8. Adenovirus Stocks 9
Adenovirus Manual 1 Table of Contents Large Scale Prep 2 Quick MOI Test 4 TCID 50 Titration 5 Infection of MNT-1 Cells 8 Adenovirus Stocks 9 CAUTION: Always use filter tips and bleach everything!!! Adenovirus
More informationFORMULATION, SENSORY EVALUATION AND NUTRIENT ANALYSIS OF PRODUCTS WITH ALOE VERA
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Joy K et al. Volume 2, Issue 6, 5321-5328. Research Article ISSN 2278 4357 FORMULATION, SENSORY EVALUATION AND NUTRIENT ANALYSIS OF PRODUCTS WITH ALOE
More informationWeds. Date. Aug. 26. Sept. 2
Mt.SanAntonioCollege Microbiology 22 Lab Schedule for Fall 2015 Mon./ Split Lab Sections ONLY Wk. Mon. 1 Aug. 24 Orientation with Introductions & Safety Rules/Regulations 2 Aug. 31 Exercise #1: The Microscope
More informationCoenzyme Q-10 Effects on Mammalian Cell Behavior. Clayton Gentilcore Grade 11 Central Catholic High School PJAS 2013
Coenzyme Q-10 Effects on Mammalian Cell Behavior Clayton Gentilcore Grade 11 Central Catholic High School PJAS 2013 An Overview of Stem Cells Unspecialized cells capable of renewing themselves through
More informationHOW TO SOLVE PRACTICAL ASPECTS OF MICROBIOLOGY
HOW TO SOLVE PRACTICAL ASPECTS OF MICROBIOLOGY PROPOSAL: NEW EXERCISES PART 2 Inés Arana, Maite Orruño & Isabel Barcina Department of Immunology, Microbiology and Parasitology University of Basque Country
More informationThe Human Microbiome Christine Rodriguez, Ph.D. Harvard Outreach 2012
The Human Microbiome Christine Rodriguez, Ph.D. Harvard Outreach 2012 Microbes are all over us Page 1 of 22 There are millions of microbes per square inch on your body Thousands of different species on
More informationHuman Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set
Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Catalog Number : SEK11233 To achieve the best assay results, this manual must be read carefully before using this product
More informationEFFECT OF OXY-WASH ON MOISTURE LOSS AND HATCHABILITY OF BROILER EGGS
EFFECT OF OXY-WSH ON MOISTURE LOSS ND HTCHBILITY OF BROILER EGGS NOTE: [O 2 XY-Wash is a analog of quatize (about 67% as concentrated).] Investigator: Location: Sponsor of Study: SUMMRY 9 February 1996
More informationHuman ipsc-derived Ventricular Cardiomyocytes. Protocol version 3.1
Human ipsc-derived Ventricular Cardiomyocytes Protocol version 3.1 Protocol version 3.1 Table of Contents Product Information 2 Recommendations 2 Preparing Cardiomyocyte Maintenance Medium 3 Cardiomyocyte
More informationInternational Journal of Scientific & Engineering Research, Volume 7, Issue 8, August ISSN
International Journal of Scientific & Engineering Research, Volume 7, Issue 8, August-2016 105 Antimicrobial activity of Andrographis paniculata stem extracts. S.Gurupriya 1 and Dr.L.Cathrine 2 1 M.phil
More informationPreservative A15 Safe antimicrobial for cosmetics and pharmaceuticals
Safe antimicrobial for cosmetics and pharmaceuticals 1. Chemical structure Structural formula: Empirical formula: C 11 16 8 8 Molecular weight: 388,29 2. Codex and names CTFA/ICI name: Imidazolidinyl Urea
More information320 MBIO Microbial Diagnosis. Aljawharah F. Alabbad Noorah A. Alkubaisi 2017
320 MBIO Microbial Diagnosis Aljawharah F. Alabbad Noorah A. Alkubaisi 2017 Pathogens of the Urinary tract The urinary system is composed of organs that regulate the chemical composition and volume of
More informationModule Four: The GI System Module Five: The Gut Microbiome. The GI System. LLiana Shanti, CN
Module Four: The GI System Module Five: The Gut Microbiome The GI System LLiana Shanti, CN www.healthmasteryinstitute.com Review Review Review The Gut Hippocrates has been quoted as saying death sits in
More information4-3 Infection and Response Biology
4-3 Infection and Response Biology. Pathogens are disease causing microorganisms. Draw one line from each disease to the correct disease-causing microorganism. [3 marks] Disease Measles Microorganism Virus
More informationMicrobes as Agents of Infectious Disease
Microbes as Agents of Infectious Disease Normal Flora Virulence and Pathogenicity Toxicity vs. Invasiveness WE ARE NOT ALONE! We are outnumbered. The average human contains about 10 trillion cells. On
More informationThe textile material is goods carrier of various types
AJHS Asian Journal of Home Science Volume 9 Issue 1 June, 2014 308-312 e ISSN-0976-8351 Open Access - www.researchjournal.co.in A Case Study Extraction and application of antimicrobial agents using neem
More informationCharles P. Gerba Departments of Soil, Water and Environmental Science and Epidemiology and Environmental Health University of Arizona Tucson, AZ
Charles P. Gerba Departments of Soil, Water and Environmental Science and Epidemiology and Environmental Health University of Arizona Tucson, AZ Percentage of Disease by Transmission Route Animal to human
More informationGB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE
Translated English of Chinese Standard: GB4789.30-2016 www.chinesestandard.net Buy True-PDF Auto-delivery. Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.30-2016
More informationKey Stage 2 Science PSHE English Estimated Teaching Time
Key Stage 2 Science Working Scientifically Animals Including Humans (Upper KS2 only) PSHE Core Theme 1: Health and Wellbeing English Reading and Comprehension Estimated Teaching Time 50 minutes The Spread
More informationPluricyte Cardiomyocytes
Pluricyte Cardiomyocytes Manual Version 2.1 / March 2018 Contents 1. Introduction 2 2. Equipment, Materials and Reagents 3 3. Methods 4 3.1 Coating of tissue culture plates 4 3.2 Thawing Pluricyte Cardiomyocytes
More information