Introduction of Tangential Flow Filtration (TFF) Karen Chan 16 May 2017

Size: px
Start display at page:

Download "Introduction of Tangential Flow Filtration (TFF) Karen Chan 16 May 2017"

Transcription

1 Introduction of Tangential Flow Filtration (TFF) Karen Chan 16 May 2017

2 What you will learn TFF principles and applications in mammalian cell processes TFF vocabulary definitions and key process parameters TFF membrane selection 2

3 Types of Filtration Normal Flow Filtration (NFF) Cartridge or Dead-ended Filtration Flow is perpendicular to the filter media All fluid passes through the media Particles are retained in/on filter 3

4 Normal Flow Filtration Fluid Flow FILTER SURFACE 4 Operate Tangential Flow Filtration Process Equipment rev 4 May 2016

5 Types of Filtration Normal Flow Filtration (NFF) Cartridge or Dead-ended Filtration Flow is perpendicular to the filter media All fluid passes through the media Particles are retained in/on filter Tangential Flow Filtration (TFF) Cross-flow Filtration Flow is tangential (parallel) to the filter surface A small percentage of the fluid flows through the filter media Retained particles are swept away from filter surface 5

6 Tangential Flow Filtration Bulk Concentration C b Liquid Tangential Flow Wall Concentration Cw MEMBRANE SURFACE 6 Operate Tangential Flow Filtration Process Equipment rev 4 May 2016

7 Applications of TFF in the BioPharm Industry Upstream Cell Harvesting (concentration) - Bacterial or yeast cells (product is intra-cellular) Cell Culture Clarification - Mammalian cell (product is extra-cellular) Downstream Protein Concentration Buffer Exchange (Diafiltration) 7

8 Example of a Mammalian Cell Process 8

9 Basic TFF Applications Clarification - product passes through the membrane - larger particles / molecules retained by membrane Concentration - product retained by the membrane - solvent (buffer) passes through the membrane Diafiltration (Buffer Exchange) - product retained by the membrane - solvent (buffer) passes through the membrane - new solvent added to product 9

10 What you will learn TFF principles and applications in mammalian cell processes TFF vocabulary definitions and key process parameters TFF membrane selection 10

11 Generic TFF System Buffer Tank Feed Tank V o, C o Air Supply L P R Transfer Pump Q T Recycle Tank V R, C b P F P f Q f Feed (Recycle) Pump Q F Product Recovery TFF Device 11

12 TFF Module Feed Pressure Retentate Pressure Feed Flow Membrane Permeate Pressure Permeate Channel Feed Channel (Screened or Open) Permeate Channel Permeate Flow Retentate Flow 12

13 Channel Flows (QF, QR, Qf) Feed Pressure Q F = Feed Flow (L/hr) Permeate Channel Feed Channel (Screened or Open) Permeate Channel Retentate Pressure Q R = Retentate Flow (L/hr) Membrane Permeate Pressure Q f = Permeate Flow (L/hr) 13

14 Pressure Drop P [bar or psi] = P F P R Difference in pressure along membrane feed channel Resistance to feed flow in the channel Function of viscosity, feed flowrate, channel geometry Feed Pressure (P F ) Retentate Pressure (P R ) Feed Channel (Screened or Open) 30 psi 20 psi Permeate Channel Permeate Pressure 2 psi P = psi = 10 psi 14

15 Transmembrane Pressure (TMP) TMP [bar or psi] = [(P F + P R ) / 2 - P f ] Average driving force across the membrane Created by applying retentate pressure Feed Pressure (P F ) Retentate Pressure (P R ) Feed Channel (Screened or Open) 30 PSI Permeate Channel 20 PSI Permeate Pressure (P f ) 2 PSI TMP = ( )/2-2 = 23 psi 15

16 Flux (J) J [Lm -2 h -1 ]= Q f / Membrane Area (time) Flux (J): permeate flow rate normalized for area of membrane it passes through Production capacity of the membrane Inlet Pressure Retentate Pressure Feed Channel (Screened or Open) 30 psi Permeate Channel 20 psi Permeate Pressure 2 psi J J = 2 L/min per 2.5 m² = 48 LMH 16

17 Membrane Polarization Concentration gradient from Cb at center of feed channel to more concentrated protein at Cw Reversible (varies with operating conditions) membrane Q F C b Q R C w membrane TMP Q f C f Q F = feed flow rate [L h -1 ] Q f = filtrate flow rate [L h -1 ] P F = feed pressure [bar] P R = retentate pressure[bar] P f = filtrate pressure [bar] C b = protein concentration in bulk solution [g L -1 ] C w = protein concentration at membrane [g L -1 ] C f = protein concentration in filtrate [g L -1 ] 17

18 Membrane Polarization No Membrane Polarization Controlled Membrane Polarization Uncontrolled Membrane Polarization 18

19 19 Polarization Mechanism and Flux

20 Polarization, TMP and Feed Flow Key point: Maintain membrane performance by balancing polarization (particle deposition) and removal TMP is the driving force creating flux Crossflow provides a sweeping cleaning action across the membrane surface Also affected by temperature and concentration TMP Crossflow 20

21 Retention & Passage Retentate Permeate % Retention % Passage % Passage = [ ] permeate [ ] retentate x 100 % Retention = % Passage Retention of protein X = (1 g/l permeate/100 g/l retentate) x 100 = 99 % 21

22 Volumetric Concentration Factor (VCF) VCF = V initial V final V initial 20 L of feedstock are ultrafiltered until 18 L have passed through the filtrate VCF = 20 L/ 2 L = 10 fold concentration V final Start Finish 22

23 Protein Concentration Factor (CF) CF = Final Protein concentration Initial Protein concentration V initial 20 L of feedstock are ultrafiltered until 18 L have passed through the filtrate VCF = 20 L/ 2 L = 10 fold concentration Start This 20 L feedstock contained 5 g/l of product and a concentration of 48 g/l was found in the retentate CF = 48 / 5 = 9.6 V final Finish 23

24 Diafiltration after concentration wash retentate with buffer PURIFICATION when is product YIELD ENHANCEMENT when is contaminant Example : Product is albumin and Contaminant is NaCl 24

25 Yield Yield = product recovered at end of process loss to permeate adsorption to membrane unrecoverable product due to hardware limitations V final Finish 25

26 What you will learn TFF principles and applications in mammalian cell processes TFF vocabulary definitions and key process parameters TFF membrane selection 26

27 Microfiltration Membrane Structure Micron pore size µm NMWL kd kd Separate cells and cell debris from proteins Durapore (PVDF) Durapore GVVP 0.2um magnified 650x 27

28 Ultrafiltration Membrane Structure Micron pore size µm NMWL - 1 kd kd Separate proteins from low molecular weight contaminants Ultracel (regenerated cellulose) Biomax (polyethersulfone) Biomax 30 kd magnified 1000x 28

29 TFF Membranes - Chemistry Membrane Chemistry Microfiltration - PVDF (Durapore ) - Polyethersulfone Ultrafiltration - Low Binding Polyethersulfone (Biomax ) - Composite Regenerated Cellulose (Ultracel ) Durapore GVVP 0.2um magnified 650x 29

30 Ultrafiltration Membrane Structure Classical membrane with voids Biomax membrane with homogenous microporous support 30

31 Destructive Test : UF Membrane Qualification UF membrane are labelled by "Nominal Molecular Weight Limit" (NMWL) Does it specify the membrane? NO! Why? - There is no standard definition for NMWL among manufacturers Typically suppliers uses Protein markers 31

32 Destructive Test : UF Membrane Qualification : Mixed Dextran Mixed Dextran testing uses a polydisperse mixture of Dextran molecules and maps the entire retention profile of the membrane allowing better reproducibility batch-to-batch tighter specifications meaningful QA/QC release But, there is NO correlation between retention and non-destructive integrity test results 32

33 Choices in Selecting a Filter Module type Channel turbulence promoter (screen) Membrane pore size Membrane material 33

34 TFF Filter Types 34

35 Filter Module Selection Flat-Plate (Cassette) - most prevalent for bioprocessing, especially MAbs - efficient packing density and mass transfer - wide choice of membranes and screens - simple linear scale-up and scale-down Spiral - good lower-cost option for very large operations Hollow Fiber - requires high pumping rates for effective mass transfer Stirred Cell - useful for screening studies only 35

36 TFF Device Characteristics Pellicon 2 and Pellicon 3 Cassettes Flat Sheet Membrane Device Linear Scalability Screened Retentate Flow Channel Highest Efficiency - highest flux at given cross-flow velocity in feed channel Smallest Working Volume and Hold-up Volume Minimal Floor Space 36

37 Filter Pore Size Rules of Thumb For a product that will be retained, use: NMWL = 1/3-1/5 product size target a product retention > Example: 150kD MAb should use 30kD membrane For a product passing to filtrate, use: NMWL = 3-5X product size target a product retention <

38 Selecting a Membrane for a Separation Understand the solvent ph Ionic strength Viscosity Processing Conditions Prefiltration / Pretreatment Temperature Time / Frequency Hardware restrictions Cleaning / Sanitizing chemical selection Personnel Restrictions 38

39 Some Membrane Selection Questions Will it be UF / MF? Solute size / characteristics Membrane performance What is the required solvent compatibility? Solvent type / concentration Are there any issues with cleaning chemicals Compatibility / Exposure / Validation What are the membrane binding / adsorption characteristics Initial & final protein concentration is important What are the yield / retention issues? Remember UF membranes are not totally retentive Establish acceptable standards first 39

40 Summary We have reviewed TFF principles and applications in mammalian cell processes TFF vocabulary definitions and key process parameters TFF membrane selection 40

Concentration and diafiltration of cell-derived, live influenza virus using 750 C hollow fiber filter cartridge

Concentration and diafiltration of cell-derived, live influenza virus using 750 C hollow fiber filter cartridge GE Healthcare Life Sciences Application note 29-928-26 AA Cross flow filtration Concentration and diafiltration of cell-derived, live influenza virus using 75 C hollow fiber filter cartridge This application

More information

THE ART TO CLEAR SOLUTIONS

THE ART TO CLEAR SOLUTIONS THE ART TO CLEAR SOLUTIONS FOOD AND BEVERAGE BEVERAGES Proteins and more... ln recent years membrane filtration as a modern and innovative technology has undergone significant developments and meanwhile

More information

Control of irreversible fouling by application of dynamic membranes

Control of irreversible fouling by application of dynamic membranes Desalination 192 (2006) 63 67 Control of irreversible fouling by application of dynamic membranes M.T. Pessoa de Amorim*, Ilia Rosa Afonso Ramos Department of Textile Engineering, University of Minho,

More information

MMS AG Membrane Systems Milk Fractionation

MMS AG Membrane Systems Milk Fractionation MMS AG Milk Fractionation George Bou-Habib MMS - Company background Founded in June 1995 Based in Zürich, Switzerland. 30 Employees In-house process development, engineering and construction. Industrial

More information

Cross Flow Sampler Fouling Index

Cross Flow Sampler Fouling Index FINAL PROJECT REPORT Cross Flow Sampler Fouling Index Co-Principal Investigators: Dr. Samer Adham, MWH Americas, Inc. Dr. Anthony Fane, University of New South Wales NWRI Final Project Report Cross Flow

More information

Journal of Membrane Science & Research

Journal of Membrane Science & Research Journal of Membrane Science and Research 3 (2017) 102-108 Journal of Membrane Science & Research journal homepage: www.msrjournal.com Research Paper Glycerin Removal from Ultrafiltration Flat Sheet Membranes

More information

Physiology of Blood Purification: Dialysis & Apheresis. Outline. Solute Removal Mechanisms in RRT

Physiology of Blood Purification: Dialysis & Apheresis. Outline. Solute Removal Mechanisms in RRT Physiology of Blood Purification: Dialysis & Apheresis Jordan M. Symons, MD University of Washington School of Medicine Seattle Children s Hospital Outline Physical principles of mass transfer Hemodialysis

More information

The Study of Dynamic Milk Ultrafiltration Performance Influenced by Membrane Molecular Weight Cut off

The Study of Dynamic Milk Ultrafiltration Performance Influenced by Membrane Molecular Weight Cut off Iran. J. Chem. Chem. Eng. Vol. 26, No.1, 27 The Study of Dynamic Milk Ultrafiltration Performance Influenced by Membrane Molecular Weight Cut off azavi, Sayed Mohammad Ali* + Department of Food Science

More information

Novel ceramic membranes for water purification and food industry Boffa, Vittorio

Novel ceramic membranes for water purification and food industry Boffa, Vittorio Aalborg Universitet Novel ceramic membranes for water purification and food industry Boffa, Vittorio Published in: PPM 2013 abstracts Publication date: 2013 Document Version Early version, also known as

More information

FOULING RESISTANT REVERSE OSMOSIS MEMBRANES. Ja-young Koo, Sung Pyo Hong, Hoon Hyung, Young Hun Kim, Sungro Yoon, Soon Sik Kim

FOULING RESISTANT REVERSE OSMOSIS MEMBRANES. Ja-young Koo, Sung Pyo Hong, Hoon Hyung, Young Hun Kim, Sungro Yoon, Soon Sik Kim FOULING RESISTANT REVERSE OSMOSIS MEMBRANES Ja-young Koo, Sung Pyo Hong, Hoon Hyung, Young Hun Kim, Sungro Yoon, Soon Sik Kim Filter Business Division Saehan Industries, Inc, 1 Jungsandong, Kyungsan city,

More information

Data File. Sephadex ion exchange media. Ion exchange chromatography. Introduction. Sephadex ion exchangers General description

Data File. Sephadex ion exchange media. Ion exchange chromatography. Introduction. Sephadex ion exchangers General description A m e r s h a m B i o s c i e n c e s Sephadex ion exchange media Data File Ion exchange chromatography Based on well documented and well proven Sephadex base matrix Simple and economical to use Very high

More information

Introduction to Membrane

Introduction to Membrane Heinrich Strathmann Introduction to Membrane Science and Technology WILEY- VCH WILEY-VCH Verlag GmbH & Co. KG aa V Preface Symbols XIII XV 1 Introduction 2 1.1 Overview of Membrane Science and Technology

More information

Influence of External Coagulant Water Types on the Performances of PES Ultrafiltration Membranes

Influence of External Coagulant Water Types on the Performances of PES Ultrafiltration Membranes 30 Journal of Membrane and Separation Technology, 2012, 1, 30-34 Influence of External Coagulant Water Types on the Performances of PES Ultrafiltration Membranes Jing He, Lingyun Ji and Baoli Shi * Polymer

More information

ASSAYING OF THE FILTRATION PARAMETERS OF WHEY BY DIFFERENT FILTRATION SYSTEMS

ASSAYING OF THE FILTRATION PARAMETERS OF WHEY BY DIFFERENT FILTRATION SYSTEMS Bulletin of Engineering Tome VII [2014] ISSN: 2067 3809 1. Szilárd SZÉLPÁL, 2. Zsuzsanna KOHÁNY, 3. Eszter FOGARASSY, 4. József CSANÁDI, 5. Gyula VATAI, 6. Cecília HODÚR ASSAYING OF THE FILTRATION PARAMETERS

More information

BabyBio IMAC columns DATA SHEET DS

BabyBio IMAC columns DATA SHEET DS BabyBio IMAC columns DATA SHEET DS 45 655 010 BabyBio columns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged)

More information

Successful Operation of Electrophoretic Lacquers

Successful Operation of Electrophoretic Lacquers Successful Operation of Electrophoretic Lacquers Surindar S Tulsi* It is almost 20 years when a viable process to deposit cathodically electrophoretic clear lacquer was introduced. This process was epoxy

More information

EFFECT OF DIFFERENT DEGUMMING PROCESSES ON MINOR COMPOUNDS IN CANOLA CRUDE OIL

EFFECT OF DIFFERENT DEGUMMING PROCESSES ON MINOR COMPOUNDS IN CANOLA CRUDE OIL EFFECT OF DIFFERENT DEGUMMING PROCESSES ON MINOR COMPOUNDS IN CANOLA CRUDE OIL Campos, L. A; Chiu, M. C; Basso, R. C; Viotto, L. A; Grimaldi, R; Gonçalves, L. A. G. Luciene Ara Campos UNICAMP Fats and

More information

Filtration Approaches to Sample Preparation and Cleanup. Golnar Javadi Application Engineer Sample Preparation Products

Filtration Approaches to Sample Preparation and Cleanup. Golnar Javadi Application Engineer Sample Preparation Products Filtration Approaches to Sample Preparation and Cleanup Golnar Javadi Application Engineer Sample Preparation Products Spp-support@agilent.com 1 Today s Agenda How does filtration work a brief overview

More information

Anti-(bio)fouling composite membranes by Polyacrylic acid/poly(vinyl alcohol) electrospun layer

Anti-(bio)fouling composite membranes by Polyacrylic acid/poly(vinyl alcohol) electrospun layer Anti-(bio)fouling composite membranes by Polyacrylic acid/poly(vinyl alcohol) electrospun layer Berta Díez Odriozola Chemical Engineering Department Universidad de Alcalá Madrid Spain Introduction NEW

More information

A REVIEW ON USING MEMBRANE REACTORS IN ENZYMATIC HYDROLYSIS OF CELLULOSE

A REVIEW ON USING MEMBRANE REACTORS IN ENZYMATIC HYDROLYSIS OF CELLULOSE Journal of Engineering Science and Technology Vol. 12, No. 4 (2017) 1129-1152 School of Engineering, Taylor s University A REVIEW ON USING MEMBRANE REACTORS IN ENZYMATIC HYDROLYSIS OF CELLULOSE THAOTHY

More information

TABLE OF CONTENT CHAPTER CONTENT PAGE

TABLE OF CONTENT CHAPTER CONTENT PAGE vii TABLE OF CONTENT CHAPTER CONTENT PAGE TITLE PAGE DECLARATION DEDICATION ACKNOWLEDGEMENT ABSTRACT ABSTRAK TABLE OF CONTENT LIST OF TABLES LIST OF FIGURES NOMENCLATURE LIST OF APPENDICES i ii iii iv

More information

Application Note USD 2916 (1) Purification of Influenza Virus by Ion Exchange Chromatography on Mustang Q XT Membranes

Application Note USD 2916 (1) Purification of Influenza Virus by Ion Exchange Chromatography on Mustang Q XT Membranes Application Note USD 2916 (1) Purification of Influenza Virus by Ion Exchange Chromatography on Mustang Q XT Membranes Fast Purification Conditions Screening on AcroPrep Advance 96-Well Filter Plates Summary

More information

PRODUCTION OF MICELLAR CASEIN CONCENTRATES USING CERAMIC MICROFILTRATION MEMBRANES: OPTIMAL PROCESS DESIGN AND SYSTEM OPERATION.

PRODUCTION OF MICELLAR CASEIN CONCENTRATES USING CERAMIC MICROFILTRATION MEMBRANES: OPTIMAL PROCESS DESIGN AND SYSTEM OPERATION. PRODUCTION OF MICELLAR CASEIN CONCENTRATES USING CERAMIC MICROFILTRATION MEMBRANES: OPTIMAL PROCESS DESIGN AND SYSTEM OPERATION A Dissertation Presented to the Faculty of the Graduate School of Cornell

More information

Use of Capto ViralQ for the removal of genomic DNA from influenza virus produced in MDCK cells

Use of Capto ViralQ for the removal of genomic DNA from influenza virus produced in MDCK cells GE Healthcare Application note 28-9769-69 AA Vaccines Use of Capto ViralQ for the removal of genomic DNA from influenza virus produced in MDCK cells We have developed a chromatographic method to remove

More information

Preparation and Application of SPPEES-TiO 2 Composite Micro-porous UF Membrane for Refinery Effluent Treatment

Preparation and Application of SPPEES-TiO 2 Composite Micro-porous UF Membrane for Refinery Effluent Treatment International Journal of Environmental Research and Development. ISSN 2249-3131 Volume 4, Number 2 (2014), pp. 147-152 Research India Publications http://www.ripublication.com/ijerd.htm Preparation and

More information

Separation of Polyphenolics and Sugar by Ultrafiltration: Effects of Operating Conditions on Fouling and Diafiltration

Separation of Polyphenolics and Sugar by Ultrafiltration: Effects of Operating Conditions on Fouling and Diafiltration Separation of Polyphenolics and Sugar by Ultrafiltration: Effects of Operating Conditions on Fouling and Diafiltration Diqiao S. Wei, M. Hossain, and Zaid S. Saleh Abstract Polyphenolics and sugar are

More information

Study on the retentivity of the volatile components of simulated guava juice using ultrafiltration

Study on the retentivity of the volatile components of simulated guava juice using ultrafiltration ORIGINAL ARTICLE Study on the retentivity of the volatile components of simulated guava juice using ultrafiltration Saad Hashim Khalil 1, Mohamed Kheireddine Aroua 2, and Nik Meriam Sulaiman 3 Abstract

More information

1 Development of Membrane Processes 1 K. Smith. 1.1 Historical background Basic principles of membrane separations 3

1 Development of Membrane Processes 1 K. Smith. 1.1 Historical background Basic principles of membrane separations 3 1 Development of Membrane Processes 1 K. Smith 1.1 Historical background 1 1.2 Basic principles of membrane separations 3 1.2.1 Depth versus screen filters 3 1.2.2 Isotropic versus anisotropic membranes

More information

Microfiltration Membranes

Microfiltration Membranes Data Sheet Product Selection Guide Microfiltration Microfiltration for Filtration and Venting Applications Microfiltration for Filtration and Venting Microfiltration (MF) is the process of separating streams

More information

3. Valorization of olive oil waste streams by the development of thin film composite membranes for selective removal of polyphenols BGU, Dr. C.

3. Valorization of olive oil waste streams by the development of thin film composite membranes for selective removal of polyphenols BGU, Dr. C. 3. Valorization of olive oil waste streams by the development of thin film composite membranes for selective removal of polyphenols BGU, Dr. C. Linder Development Objectives Cost effective Extraction of

More information

Filtration Technology

Filtration Technology Filtration Technology UF & MF as the Means to Improve Cheese Makes & Standardize Yields November 15, 2016 Milk Molitor Wisconsin Center for Dairy Research Center for Dairy Research Solution Based Research

More information

TECHNICAL DATA SHEET GROWCLEAR PLUS

TECHNICAL DATA SHEET GROWCLEAR PLUS TECHNICAL DATA SHEET GROWCLEAR PLUS CATHODIC ELECTROPHORETIC CLEAR / TINTED LACQUERING PROCESS Introduction Growclear Plus is an advanced version of the earlier popular electrophoretic Clearlyte lacquer.

More information

Food Ingredients. Equipment and Solutions for Food Ingredients and Starch

Food Ingredients. Equipment and Solutions for Food Ingredients and Starch Food Ingredients Equipment and Solutions for Food Ingredients and Starch 2 food ingredients Quality ingredients for competitive products As a trusted supplier of customized and innovative process solutions,

More information

Permeation of organic molecules in water and ethanol-water solutions by reverse osmosis

Permeation of organic molecules in water and ethanol-water solutions by reverse osmosis Proceedings of European Congress of Chemical Engineering (ECCE-6) Copenhagen, 16-20 September 2007 Permeation of organic molecules in water and ethanol-water solutions by reverse osmosis J. Labanda, J.

More information

The Effects of Antifoams on Ultrafiltration Membranes

The Effects of Antifoams on Ultrafiltration Membranes The Effects of Antifoams on Ultrafiltration Membranes By Jean-Paul Lecomte Dow Corning Corporation Dow Corning Silicone Solutions As the pioneer of silicon-based technology, Dow Corning has been improving

More information

TANNERY PROCESS REGULATION WITH ON LINE CHROMIUM (III) MONITORING

TANNERY PROCESS REGULATION WITH ON LINE CHROMIUM (III) MONITORING II WORKSHOP ON CHEMICAL SENSORS AND BIOSENSORS TANNERY PROCESS REGULATION WITH ON LINE CHROMIUM (III) MONITORING Massimo Pizzichini 1, Mauro Spadoni 1, Rosalba Montani 2. 1 ENEA, C.R. Casaccia, Via Anguillarese

More information

Vol. 10, No ABSTRACT

Vol. 10, No ABSTRACT EFFECTS OF ENZYME TREATMENT ON THE MICROFILTRATION OF ELDERBERRY 1,3 D. Furulyás, 2 F. Savanya, 2 Sz. Bánvölgyi, 3 N. Papp, 3 É. Stefanivots-Bánya, 1 M. Stéger-Máté 1 Department of Food Preservation, Faculty

More information

Depleting Lipoproteins from Serum

Depleting Lipoproteins from Serum Depleting Lipoproteins from Serum Kathy K. Foxx Kalen Biomedical LLC President For decades, fetal bovine serum (FBS) has been used as a supplement for cell-culture media, providing the growth factors that

More information

Biochemical Techniques 06 Salt Fractionation of Proteins. Biochemistry

Biochemical Techniques 06 Salt Fractionation of Proteins. Biochemistry . 1 Description of Module Subject Name Paper Name 12 Module Name/Title 2 1. Objectives Understanding the concept of protein fractionation Understanding protein fractionation with salt 2. Concept Map 3.

More information

DEMONSTRATING IMPROVED RO SYSTEM PERFORMANCE WITH NEW LOW DIFFERENTIAL (LD) TECHNOLOGY. Abstract

DEMONSTRATING IMPROVED RO SYSTEM PERFORMANCE WITH NEW LOW DIFFERENTIAL (LD) TECHNOLOGY. Abstract DEMONSTRATING IMPROVED RO SYSTEM PERFORMANCE WITH NEW LOW DIFFERENTIAL (LD) TECHNOLOGY Rich Franks, Craig Bartels, PhD., Alex Anit, Hydranautics, Oceanside, CA. Abstract A new low differential (LD) pressure

More information

Novel technologies for blood purification Prof. Dr. Dimitrios Stamatialis

Novel technologies for blood purification Prof. Dr. Dimitrios Stamatialis Novel technologies for blood purification Prof. Dr. Dimitrios Stamatialis Biomaterials Science and Technology, MIRA institute, University of Twente 1 BST group MIRA: Technology for regeneration 5 My

More information

Ergovaline. [Methods listed in the Feed Analysis Standards] 1 Liquid chromatography Note 1, 2 [Feed Analysis Standards, Chapter 5, Section 2

Ergovaline. [Methods listed in the Feed Analysis Standards] 1 Liquid chromatography Note 1, 2 [Feed Analysis Standards, Chapter 5, Section 2 Ergovaline C 29 35 5 5 MW: 533.619 CAS o.: 2873-38-3 [Summary of ergovaline] Ergovaline is a kind of ergot alkaloids produced by endophytes eotyphodium coenophialum and eotyphodium lolli, symbionts of

More information

Ultrafiltration as a method of separation of natural organic matter from water

Ultrafiltration as a method of separation of natural organic matter from water Materials Science-Poland, Vol. 26, No. 2, 2008 Ultrafiltration as a method of separation of natural organic matter from water M. KABSCH-KORBUTOWICZ * Wrocław University of Technology, Institute of Environment

More information

TRANSPORT OF PHARMACEUTICAL AND NOM

TRANSPORT OF PHARMACEUTICAL AND NOM TRANSPORT OF PHARMACEUTICAL AND NOM IN NF AND TIGHT UF MEMBRANES Gun-Young Park *, Jaeweon Cho * Department of environmental science and engineering GIST, 1 Oryong-dong, Buk-gu, Gwangju 500-712, Korea

More information

Capturing lightning In A Bottle

Capturing lightning In A Bottle Capturing lightning In A Bottle Featured In: Manufacturing & Capabilities I Facility of the Year I ISPE By Stephen Hall, Senior Director, Engineering, MannKind Corporation; Juergen Martens, Chief Technical

More information

Sephadex G-25 resins and prepacked formats

Sephadex G-25 resins and prepacked formats GE Healthcare Desalting/buffer exchange and size exclusion chromatography Sephadex G-25 resins and prepacked formats Size exclusion chromatography (SEC) or as it is also commonly named, gel filtration

More information

Liquid-Liquid Extraction Prof. Mukesh Doble Department Of Biotechnology Indian Institute Of Technology, Madras. Lecture - 19

Liquid-Liquid Extraction Prof. Mukesh Doble Department Of Biotechnology Indian Institute Of Technology, Madras. Lecture - 19 Liquid-Liquid Extraction Prof. Mukesh Doble Department Of Biotechnology Indian Institute Of Technology, Madras Lecture - 19 Liquid-Liquid Extraction Let us continue with the Liquid- Liquid Extraction.

More information

Examination of Whey Degreasing by Modified Membrane Filtration

Examination of Whey Degreasing by Modified Membrane Filtration Journal of Agricultural Science and Technology B 6 (2016) 57-66 doi: 10.17265/2161-6264/2016.01.007 D DAVID PUBLISHING Examination of Whey Degreasing by Modified Membrane Filtration Szilárd Szélpál 1,

More information

REMOVAL OF COLOR FROM THE RAW SUGAR MANU- FACTURING PROCESS BY MEMBRANE TREATMENT

REMOVAL OF COLOR FROM THE RAW SUGAR MANU- FACTURING PROCESS BY MEMBRANE TREATMENT REMVAL F CLR FRM THE RAW SUGAR MANU- FACTURING PRCESS BY MEMBRANE TREATMENT Chalongsri Flood * and Adrian E. Flood Received: Mar 6, 2006: Revised: Apr 12, 2006; Accepted: Apr 18, 2006 ABSTRACT Color removal

More information

FILTREC SUCTION FILTERS SECTION 19B. FilTers. FA-1 suction spin-on Fs-1 suction strainers Fs-7 in tank mounted suction strainers...

FILTREC SUCTION FILTERS SECTION 19B. FilTers. FA-1 suction spin-on Fs-1 suction strainers Fs-7 in tank mounted suction strainers... FILTREC SUCTION FILTERS SECTION 19B suction FilTers FA-1 suction spin-on...688 Fs-1 suction strainers...694 Fs-7 in tank mounted suction strainers...699 50% off all prices 636-770 Harrier Catalogue 14

More information

Mass Transfer, Fluid Flow and Membrane Properties in Flat and Corrugated Plate Hyperfiltration Modules

Mass Transfer, Fluid Flow and Membrane Properties in Flat and Corrugated Plate Hyperfiltration Modules Desalination, 60 (1986) 213-222 213 Elsevier Science Publishers B.V., Amsterdam -- Printed in The Netherlands Mass Transfer, Fluid Flow and Membrane Properties in Flat and Corrugated Plate Hyperfiltration

More information

Study of the Effect of Nanoparticles and Surface Morphology on Reverse Osmosis and Nanofiltration Membrane Productivity

Study of the Effect of Nanoparticles and Surface Morphology on Reverse Osmosis and Nanofiltration Membrane Productivity Membranes 203, 3, 96-225; doi:0.3390/membranes303096 Article OPEN ACCESS membranes ISSN 2077-0375 www.mdpi.com/journal/membranes Study of the Effect of Nanoparticles and Surface Morphology on Reverse Osmosis

More information

Flume Installation Instructions

Flume Installation Instructions Flume Installation Instructions To ensure a successful installation, it is important to keep the following points in mind: 1) There should be no bend, dips, elbows, or flow junction immediately upstream

More information

Column chromatography for the development of pandemic and seasonal influenza vaccines

Column chromatography for the development of pandemic and seasonal influenza vaccines Column chromatography for the development of pandemic and seasonal influenza vaccines Olga Chervyakova Research Institute for Biological Safety Problems, RK ME&S 5 th Meeting with International Partners

More information

(12) Patent Application Publication (10) Pub. No.: US 2015/ A1

(12) Patent Application Publication (10) Pub. No.: US 2015/ A1 (19) United States US 20150230484A1 (12) Patent Application Publication (10) Pub. No.: US 2015/0230484 A1 Döring (43) Pub. Date: (54) LACTOSE-FREE MILK PRODUCTS (52) U.S. Cl. CPC... A23C 9/1206 (2013.01)

More information

November 2015 Volume XXIX No. 2

November 2015 Volume XXIX No. 2 November 2015 Volume XXIX No. 2 Effect of Soluble Calcium and Lactose on Limiting Flux and Serum Protein Removal During Skim Milk Microfiltration M.C. Adams, E.E. Hurt & D.M. Barbano J. of Dairy Sci. 98(11):

More information

Effect of Membrane Properties on Performance of Membrane Distillation for Ammonia Removal

Effect of Membrane Properties on Performance of Membrane Distillation for Ammonia Removal Effect of Membrane Properties on Performance of Membrane Distillation for Ammonia Removal Zongli Xie CSIRO Materials Science and Engineering, Private Bag 33, Clayton South, Vic.3169, Australia E-mail:

More information

Super Concentrated Coconut Water

Super Concentrated Coconut Water Super Concentrated Coconut Water Using Aquaporin Inside forward osmosis CONCENTRATE UP TO 65 BRIX UNIQUE CUTTING-EDGE PROCESS IMPROVED LOGISTICS Improved coconut water distribution logistics SUPER CONCENTRATION

More information

PEEK SS Columns & Guard Columns

PEEK SS Columns & Guard Columns PEEK SS Columns & Guard Columns ~ Stainless Steel Analytical Columns ~ PEEK Columns ID 2.1, 4.0, 4.6 & 7.5 mm ~ Jour-Guard Cartridges Stainless Steel or PEEK ~ Stainless Steel End Fittings 1/4 OD Columns

More information

Development of an Organic-Inorganic PVDF/ anoclay Ultrafiltration Composite Membrane

Development of an Organic-Inorganic PVDF/ anoclay Ultrafiltration Composite Membrane Development of an Organic-Inorganic PVDF/ anoclay Ultrafiltration Composite Membrane A.C.D. Morihama 1 and J.C. Mierwza 1 1 Escola Politécnica da Universidade de São Paulo, São Paulo, São Paulo, 05508-900,

More information

utarwllnq and JProcessina

utarwllnq and JProcessina utarwllnq and JProcessina Section Proc. Fla. State Hort. Soc. 102:146-152. 1989. MEMBRANE CONCENTRATION OF ORANGE JUICE Additional index words, Stephen Cross SeparaSystems L.P. 317 W. Brokaw Road Santa

More information

Potential of membrane separation processes in cheese whey fractionation and separation

Potential of membrane separation processes in cheese whey fractionation and separation Available online at www.sciencedirect.com Procedia Engineering 42 (2012 ) 1425 1436 20 th International Congress of Chemical and Process Engineering CHISA 2012 25 29 August 2012, Prague, Czech Republic

More information

HPLC. Varian Dynamax Preparative HPLC Columns

HPLC. Varian Dynamax Preparative HPLC Columns HPLC Varian Dynamax Preparative HPLC Columns Preparative HPLC Simple, successful and economical Linear scale-up move from analytical separation to preparative purification quickly and simply. Dynamic Axial

More information

247 CMR BOARD OF REGISTRATION IN PHARMACY

247 CMR BOARD OF REGISTRATION IN PHARMACY 247 CMR 18.00: NON-STERILE COMPOUNDING Section 18.01: Authority and Purpose 18.02: Non-Sterile Compounding Process 18.03: Non-Sterile Compounding Facility 18.04: Non-Sterile Compounding Equipment 18.05:

More information

Evaluation of anthocyanin content on blackberry juice (Rubus spp.) processed by microfiltration

Evaluation of anthocyanin content on blackberry juice (Rubus spp.) processed by microfiltration Evaluation of anthocyanin content on blackberry juice (Rubus spp.) processed by microfiltration Monteiro, F.S. a, Viotto, L.A. a, Cabral, L.M.C b a UNICAMP/FEA. Campinas-SP, Brazil (fsilva@fea.unicamp.br;

More information

Effects of membrane fouling on solute rejection during membrane filtration of activated sludge

Effects of membrane fouling on solute rejection during membrane filtration of activated sludge Process Biochemistry 36 (2001) 855 860 www.elsevier.com/locate/procbio Effects of membrane fouling on solute rejection during membrane filtration of activated sludge In-Soung Chang a, *, Soon-Ouk Bag a,

More information

Stax Disposable Depth Filters

Stax Disposable Depth Filters USD 2758 Stax Disposable Depth Filters Featuring Seitz AKS Activated Carbon Media Highest Efficiency in Adsorption and Design Powdered activated carbon (PAC) is widely used in the pharmaceutical industry

More information

PROGRESS OF OF NANOFILTRATION MEMBRANES. 1 st Korea-US Nano Forum Kew-Ho Lee

PROGRESS OF OF NANOFILTRATION MEMBRANES. 1 st Korea-US Nano Forum Kew-Ho Lee PROGRESS OF OF NANOFILTRATION MEMBRANES 1 st Korea-US Nano Forum 2003. 10. 14 Kew-Ho Lee Membrane and Separation Research Center Korea Research Institute of Chemical Technology TEL) 82-42-860-7240 FAX)

More information

Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch using Membrane Reactor

Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch using Membrane Reactor 1543 A publication of CHEMICAL ENGINEERING TRANSACTIONS VOL. 56, 2017 Guest Editors: Jiří Jaromír Klemeš, Peng Yen Liew, Wai Shin Ho, Jeng Shiun Lim Copyright 2017, AIDIC Servizi S.r.l., ISBN 978-88-95608-47-1;

More information

Sephadex G-25 media and prepacked formats

Sephadex G-25 media and prepacked formats Data File 28-9137-87 AC Desalting/buffer exchange and gel filtration Sephadex G-25 media and prepacked formats Gel filtration has successfully been employed for size based separations of macromolecules

More information

The use of dead-end and cross-flow nanofiltration to purify prebiotic oligosaccharides from reaction mixtures

The use of dead-end and cross-flow nanofiltration to purify prebiotic oligosaccharides from reaction mixtures ORIGINAL ARTICLE to purify prebiotic oligosaccharides from reaction mixtures. Alistair S. Grandison 1, Athanasios K. Goulas 2, and Robert A. Rastall 3 Abstract Grandison, A.S., Goulas, A.K., and Rastall,

More information

EASI-EXTRACT FOLIC ACID Product Code: P81 / P81B

EASI-EXTRACT FOLIC ACID Product Code: P81 / P81B EASI-EXTRACT FOLIC ACID Product Code: P81 / P81B Immunoaffinity columns for use in conjunction with HPLC or LC-MS/MS. For in vitro use only. P81/V16/13.04.15 www.r-biopharm.com Contents Page Test Principle...3

More information

QuickSplitTM Flow Splitters

QuickSplitTM Flow Splitters QuickTM Flow ters between 2 or more detectors such as Mass Spectroscopy and evaporative light scattering, UV, or IR. This is especially important when one of the detectors is destructive, such as Mass

More information

A New Era in SFC Column Technology

A New Era in SFC Column Technology B TM A ew Era in SFC Column Technology Combining state-of-the-art media manufacturing with the industry leading column technology, Viridis Supercritical Fluid Chromatography (SFC) columns bring a new level

More information

FORMULATION CHOICE. How and why they are chosen. Dr Andy Fowles On behalf of ECPA Specification Expert Group

FORMULATION CHOICE. How and why they are chosen. Dr Andy Fowles On behalf of ECPA Specification Expert Group FORMULATION CHOICE How and why they are chosen Dr Andy Fowles On behalf of ECPA Specification Expert Group Topics Why formulate? How to identify formulation options Drivers Principle formulation type overview

More information

Effect of ceramic membrane channel diameter on limiting retentate protein concentration during skim milk microfiltration 1

Effect of ceramic membrane channel diameter on limiting retentate protein concentration during skim milk microfiltration 1 J. Dairy Sci. 99:167 182 http://dx.doi.org/10.3168/jds.2015-9897 American Dairy Science Association, 2016. Effect of ceramic membrane channel diameter on limiting retentate protein concentration during

More information

Increase The Sugar Concentration of The Solution Sugar by Reverse Osmotic Membrane

Increase The Sugar Concentration of The Solution Sugar by Reverse Osmotic Membrane Journal of Physics: Conference Series PAPER OPEN ACCESS Increase The Sugar Concentration of The Solution Sugar by Reverse Osmotic Membrane To cite this article: S Redjeki et al 2018 J. Phys.: Conf. Ser.

More information

A Review of Membranes Classifications, Configurations, Surface Modifications, Characteristics and Its Applications in Water Purification

A Review of Membranes Classifications, Configurations, Surface Modifications, Characteristics and Its Applications in Water Purification Chemical and Biomolecular Engineering 2017; 2(2): 57-82 http://www.sciencepublishinggroup.com/j/cbe doi: 10.11648/j.cbe.20170202.11 A Review of Membranes Classifications, Configurations, Surface Modifications,

More information

EASIMIP TM PATULIN Product Code: P250 / P250B

EASIMIP TM PATULIN Product Code: P250 / P250B EASIMIP TM PATULIN Product Code: P250 / P250B Molecularly imprinted polymer columns for use in conjunction with HPLC. For in vitro use only. P250B/V5/03.09.18 www.r-biopharm.com Contents Page Test Principle...

More information

GE Healthcare Life Sciences. Quality matters. Whatman TM filters for air monitoring

GE Healthcare Life Sciences. Quality matters. Whatman TM filters for air monitoring GE Healthcare Life Sciences Quality matters Whatman TM filters for air monitoring Quality matters Why does quality matter? Particulate testing Examples include PM 10 & PM 2.5 particulate monitoring. Quality

More information

Removal of VOCs from aqueous solutions using pervaporation process

Removal of VOCs from aqueous solutions using pervaporation process Journal of Biodiversity and Environmental Sciences (JBES) ISSN: 2220-6663 (Print) 2222-3045 (Online) Vol. 2, No. 9, p. 33-38, 2012 http://www.innspub.net RESEARCH PAPER OPEN ACCESS Removal of VOCs from

More information

109 IWA Publishing 2002 Journal of Water Supply: Research and Technology AQUA

109 IWA Publishing 2002 Journal of Water Supply: Research and Technology AQUA 109 IWA Publishing 2002 Journal of Water Supply: Research and Technology AQUA 51.2 2002 Effects of molecular weight cutoff, f/k ratio (a hydrodynamic condition), and hydrophobic interactions on natural

More information

Final Report. Department of Water Resources. Project: Role of Microfiltration (MF) Cake Layer Composition and Stability in Desalination Efficiency

Final Report. Department of Water Resources. Project: Role of Microfiltration (MF) Cake Layer Composition and Stability in Desalination Efficiency Final Report Department of Water Resources Project: Role of Microfiltration (MF) Cake Layer Composition and Stability in Desalination Efficiency Submitted by: Jana Safarik and Donald W. Phipps, Jr. Research

More information

Separation of d-limonene from supercritical CO 2 by means of membranes

Separation of d-limonene from supercritical CO 2 by means of membranes J. of Supercritical Fluids 34 (2005) 143 147 Separation of d-limonene from supercritical CO 2 by means of membranes Luiz Henrique Castelan Carlson, Ariovaldo Bolzan, Ricardo Antônio Francisco Machado Universidade

More information

Effects of viscosity, pump mechanism and nozzle geometry on nasal spray droplet size

Effects of viscosity, pump mechanism and nozzle geometry on nasal spray droplet size ILASS Europe 21, 23rd Annual Conference on Liquid Atomization and Spray Systems, Brno, Czech Republic, September 21 P. Kippax 1, J. Suman 2, A. Virden 1* and G. Williams 3 1 Malvern Instruments, Grovewood

More information

TWO-STAGE INTEGRATED CERAMIC MEMBRANE REACTOR SYSTEM FOR THE CONTINUOUS ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES

TWO-STAGE INTEGRATED CERAMIC MEMBRANE REACTOR SYSTEM FOR THE CONTINUOUS ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES TWO-STAGE INTEGRATED CERAMIC MEMBRANE REACTOR SYSTEM FOR THE CONTINUOUS ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES Mehrdad Ebrahimi 1, Laura Placido 1, Larisa Engel 1, Kikavous Shams Ashaghi 1, Peter Czermak

More information

This document is downloaded from DR-NTU, Nanyang Technological University Library, Singapore.

This document is downloaded from DR-NTU, Nanyang Technological University Library, Singapore. This document is downloaded from DR-NTU, Nanyang Technological University Library, Singapore. Title Design and development of novel feed spacers in spiral wound membrane modules with 3D printing Author(s)

More information

Membrane filtration in water recycling: removal of natural hormones

Membrane filtration in water recycling: removal of natural hormones Membrane filtration in water recycling: removal of natural hormones L.D. Nghiem,.I. Schäfer and T.D.Waite Centre for Water and Waste Technology, School of Civil and Environmental Engineering, The University

More information

HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC

HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 129 Infinity II LC Application Note Small Molecule Pharmaceuticals Authors Gerd Vanhoenacker, Mieke Steenbeke, Koen Sandra,

More information

New method of blood purification (Recycle Filtration System)

New method of blood purification (Recycle Filtration System) Tokai J Exp Clin Med., Vol. 33, No. 3, pp. 124-129, 2008 New method of blood purification (Recycle Filtration System) Hajime SUZUKI 1), Miho HIDA 1), Makoto KITAMURA 1), Shin-ichi TANAKA 2), Takayo MIYAKOGAWA

More information

ACE CN-ES. Extra Stability. Extended Spacer. Enhanced Selectivity ACE. Combining CN polar selectivity with enhanced hydrophobicity

ACE CN-ES. Extra Stability. Extended Spacer. Enhanced Selectivity ACE. Combining CN polar selectivity with enhanced hydrophobicity ACE CN-ES Combining CN polar selectivity with enhanced hydrophobicity Extended Spacer Extra Stability New stationary phase technology for UHPLC and HPLC separations Alternative polar selectivity for method

More information

Sample Preparation Techniques for Biological Matrices: Finding the right balance to achieve optimal results

Sample Preparation Techniques for Biological Matrices: Finding the right balance to achieve optimal results Sample Preparation Techniques for Biological Matrices: Finding the right balance to achieve optimal results Presenter: Diana Wang Applications Engineer SPP-Support@agilent.com 1 Today s Agenda - Why sample

More information

NANO 243/CENG 207 Course Use Only

NANO 243/CENG 207 Course Use Only L9. Drug Permeation Through Biological Barriers May 3, 2018 Lipids Lipid Self-Assemblies 1. Lipid and Lipid Membrane Phospholipid: an amphiphilic molecule with a hydrophilic head and 1~2 hydrophobic tails.

More information

Product Data Sheet July 2014

Product Data Sheet July 2014 Product Data Sheet July 2014 INTERNATIONAL MASTER FOR PROFESSIONAL USE ONLY V0930V_E EHS Turbo Plus P498-Line and P494-Line Product Description P498 & P494-Line EHS Turbo Plus Mixing Basics/Ready Mixes

More information

Contents. 01 Welded Syringe Filter Economy Syringe Filter Sterile Syringe Filter Membrane Filter 21

Contents. 01 Welded Syringe Filter Economy Syringe Filter Sterile Syringe Filter Membrane Filter 21 C0000613 C0000613 Contents LABFIL is a product brand under ALWSCI Technologies, which provides wide selection of syringe f ilter and membranes. It is our great pleasure to have customer be satisf ied with

More information

HPLC Analysis of Sugars

HPLC Analysis of Sugars HPLC Analysis of Sugars Pre-Lab Exercise: 1) Read about HPLC, sugars and the experiment and its background. 2) Prepare a flowchart as appropriate for the lab exercise. 3) Note the various sugar concentrations

More information

Modified cellulosic nanofiltration membrane with improved characteristics for desalination and concentration of reactive dyest

Modified cellulosic nanofiltration membrane with improved characteristics for desalination and concentration of reactive dyest Indian Journal of Chemical Technology Vol.3,May 1996,pp. 131-135 Modified cellulosic nanofiltration membrane with improved characteristics for desalination and concentration of reactive dyest G Venkidachalam

More information

Environment Protection Engineering ULTRAFILTRATION OF DYE SOLUTIONS IN THE PRESENCE OF CATIONIC AND ANIONIC SURFACTANTS

Environment Protection Engineering ULTRAFILTRATION OF DYE SOLUTIONS IN THE PRESENCE OF CATIONIC AND ANIONIC SURFACTANTS Environment Protection Engineering Vol. 35 9 No. KATARZYNA MAJEWSKA-NOWAK* ULTRAFILTRATION OF DYE SOLUTIONS IN THE PRESENCE OF CATIONIC AND ANIONIC SURFACTANTS The objective of the study was to investigate

More information

QuickSplit TM Flow Splitters

QuickSplit TM Flow Splitters Quick TM Flow ters between 2 or more detecrs such as Mass Spectroscopy and evaporative light scattering, UV, or IR. This is especially important when one of the detecrs is destructive, such as Mass Spec

More information