For Far. Greater. UHPLC Column Performance You Must Have Kinetex Core-Shell UHPLC Columns.
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1 For Far Greater UHPLC Column Performance... You Must Have Kinetex Core-Shell UHPLC Columns
2 The UHPLC Core-Shell Advantage Kinetex Core-Shell Technology produces increased efficiencies over traditional, fully porous sub- columns on the market, yielding remarkable chromatographic resolution, higher peak capacities, and greater sensitivity, so labs can get the most out of every UHPLC analysis. If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product within days for a FULL REFUND.
3 The Core-Shell Advantage Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a core-shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies! Kinetex Core-Shell Fully Porous Less Band Broadening More Band Broadening Kinetex Core-Shell High particle density helps create optimal bed structure which reduces band broadening effects of Eddy Diffusion Ultra-high performance on UHPLC systems Reduced diffusion path improves efficiency Solid Core Porous Shell Fully Porous Less homogenuous bed structure leads to performance loss Diffusion path limits efficiencies Band broadening due to frictional heating as flow rate increases Phenomenex l WEB:
4 High Performance UHPLC Core-Shell Particles Kinetex. Sub- efficiencies at much lower backpressures Gives you the opportunity to increase flow rate for increased peak capacities and shorter run times Can be used on both UHPLC and HPLC systems. Kinetex. 0 % higher performance than traditional. fully porous particle First core-shell sub- on the market. Kinetex. Incredible column efficiencies above 00,000 p/m, giving an average of 0 % higher efficiencies than traditional. fully porous particles Second core-shell sub- on the market. Other Core-Shell Solutions... Kinetex While not developed specifically for UHPLC systems, the Kinetex provides or better efficiencies at backpressures and is fully scalable to the other Kinetex particle sizes! Aeris Columns Aeris WIDEPORE and PEPTIDE columns are excellent tools for UHPLC analysis of proteins and peptides! solid core.---. solid core.---. solid core
5 UHPLC Compatible Kinetex Columns are Compatible with All UHPLC Instruments Kinetex on Agilent 90 Kinetex on JASCO X-LC Kinetex on Shimadzu Nexera Kinetex on Waters ACQUITY UPLC Need help installing your Kinetex column? Waters, ACQUITY and UPLC are registered trademarks of Waters Corporation. Agilent is a registered trademark of Agilent Technologies, Inc. Shimadzu and Nexera are registered trademarks of Shimadzu Corporation. JASCO is a registered trademark of JASCO Corporation. Phenomenex is not affiliated with Agilent, JASCO, or Waters. Phenomenex l WEB:
6 Kinetex.. Beyond Fully Porous Sub- Performance With its ability to provide ultra-high performance at a fraction of the pressure, the Kinetex. paves the way for better, faster UHPLC methods. Where Your UHPLC Performance Stops... Waters ACQUITY. 0. ml/min and 9 bar Peak Capacity of Ours Starts... Kinetex. 8 9 min 9 0 App ID ml/min and bar Peak Capacity of 8 App ID 00 Conditions same for all columns except where noted: Columns: Kinetex. C8 ACQUITY UPLC BEH. C8 Dimensions: 00 x. mm Mobile Phase: A: 0.% Formic Acid in Water B: 0.% Formic Acid in Acetonitrile Gradient: Time (min) % B 9 9 Flow Rate: As Noted Detection: nm Backpressure: As Noted Instrument: Waters ACQUITY UPLC Sample:. Pyridine. Acetaminophen. Pindolol. Sulfathiazole. Acebutolol. Chloropheniramine. Benzyl Alcohol 8. Phenol 9. Triprolidine 0. -Methyl, -nitrobenzoic acid. Nortriptyline. Prednisolone. Diflunisal. -Hydroxy, -methyl benzaldehyde. Hexanophenone 0 min... And Keeps Going! Kinetex..0 ml/min and 8 bar Peak Capacity of ,9 min App ID 000 Faster Analysis Higher Performance On Your UHPLC System Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. BEH Technology is a trademark of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation. Comparative separations may not be representative of all applications.
7 Kinetex. Do More with Core-Shell! Start by matching a Kinetex. column to the sub- you re currently using. Then watch the pressure drop and you re options appear. In no time you ll have better peak capacities, sensitivities, and greater column performance. 98 Intensity, cps Intensity, cps.e.0e e.e Fully Porous. C8 00 psi ( bar) Peak Capacity =. App ID min Kinetex. C8 00 psi ( bar) Peak Capacity = min Lower backpressure gives you options App ID 99 Conditions are the same for all columns: Column: Kinetex. μm C8 Fully Porous. μm C8 Dimensions: Kinetex: 0 x. mm, 0 x. mm, and 00 x. mm Fully Porous: 0 x. mm Flow Rate: 0.8, 0.8,., and 0.8 ml/min Mobile Phase: A: 0.% Formic acid in Water B: 0.% Formic acid in Acetonitrile Gradient: Time (min) % B Temperature: 0 ºC Detection: MS Backpressure: As noted Analytes:. Haloperidol. Diltiazem. Terfenadine. Cimetidine. Acetaminophen. Sulfathiazole. Pindolol 8. Quinidine 9. Acebutolol 0. Chlorpheniramine. Tripolidine. Prednisolone. Nortriptyline. -Hydroxy--methyl benzaldehyde. Hexanophenone Increase the flow rate Kinetex. C8 98.e Increase peak capacity Increase column length Kinetex. C8.08e Increase potential resolving power Intensity, cps.0e psi ( bar) 90 Peak Capacity =. 8 App ID min Intensity, cps.00e psi ( bar) Peak Capacity = min App ID 990 Phenomenex l WEB:
8 Kinetex.. The First Sub- Core-Shell Column Upgrade the efficiency and performance of your current sub- column with a Kinetex. core-shell column. Extra resolution, higher peak capacities, and greater sensitivity are all within your reach with Kinetex Core-Shell Technology. AU % 8 % % Higher Resolution (between peaks and ) Higher Resolution (between peaks and ) Higher Resolution (between peaks and 8) AU min Waters ACQUITY BEH. C8 App ID 980 Conditions for both columns: Column: Kinetex. XB-C8 ACQUITY UPLC BEH. C8 Dimensions: 0 x. mm Mobile Phase: A: Milli-Q Water B: Acetonitrile Gradient: Time (min) % B Flow Rate: 0. ml/min Temperature: Ambient Detection: 0 nm (ambient) Backpressure: 89 bar (Kinetex) 9 bar (ACQUITY) Instrument: Waters ACQUITY UPLC Sample:. Hydrocortisone. Cortisone. Corticosterone. Cortisone--Acetate. DHEA (Dehydroepiandrosterone). -Hydroxyprogesterone. Deoxycorticosterone 8. Progesterone App ID 980 min Waters, ACQUITY, and UPLC are registered trademarks of Waters Corporation. BEH Technology is a trademark of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation. Comparative separations may not be representative of all applications.
9 Kinetex. Superior Resolving Power The increased efficiency of the Kinetex. allows you to use shorter columns for faster run times while keeping optimal performance. Now it s time for you to challenge your difficult separations with the superior efficiency and resolving power of a Kinetex. core-shell column. Fully Porous. C8 Intensity, cps min.e Fused-Core. C8 Intensity, cps min Intensity, cps.e Kinetex. Faster analysis, better sensitivity, and overall better resolution! App ID min Conditions for all columns Columns: Fully porous. C8 (0 x. mm) Fused-Core. C8 (0 x. mm) Kinetex. C8 (0 x. mm) Mobile Phase: A: 0 mm Ammonium Formate B: Acetonitrile Gradient: Time (min) % B Flow Rate: 0. ml/min Temperature: º C Detection: MS/MS (AB SCIEX API 000 ) Sample:. Lorazepam glucoronide Fused-Core is a registered trademark of Advanced Materials Technology, Inc. Phenomenex is in no way affiliated with Advanced Materials Technology, Inc. Comparative separations may not be representative of all applications. Phenomenex l WEB:
10 Kinetex.. The New Standard for UHPLC Bring your UHPLC analysis to the next level with the incredibly powerful Kinetex.. Kinetex. C8 mau Peak Capacity = 8 App ID 08 Higher Sensitivity 0 min mau Waters ACQUITY UPLC BEH. C Peak Capacity = App ID 08 Greater Resolution of Impurities 0 min Conditions for both columns: Column: Kinetex. C8 ACQUITY UPLC BEH. C8 Dimensions: 0 x. mm Mobile Phase: Acetonitrile / Water (0:0) Gradient: Time (min) % B Flow Rate: 0. ml/min Temperature: Ambient Detection: nm Instrument: Waters ACQUITY UPLC Sample:. -Gingerol (). -Gingerol (). Capsaicin. 8-Gingerol. -Shogal. 0-Gingerol. 8-Shogal 8. 0-Shogal BEH. μm Kinetex. μm Waters, ACQUITY, and UPLC are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation. Comparative separations may not be representative of all applications. 8
11 Kinetex. More Peaks and More Sensitivity, in Less Time With typically 0 % more efficiency then a fully porous. particle, the Kinetex. lets you SEE MORE! Waters ACQUITY BEH. C8 AU 0. 8 Peaks App ID Min Kinetex 9. C8 AU Peaks App ID 9 Min Conditions for all columns same except where noted: Column: Waters ACQUITY UPLC BEH. C8 Kinetex. C8 Dimensions: 0 x. mm Mobile Phase: A: 0. % TFA in Water B: 0. % TFA in Acetonitrile Gradient: Time (min) % B Flow Rate: 0. ml/min Temperature: 0 º C Detection: 0 nm (ambient) Instrument: Waters ACQUITY UPLC Sample: Trypsin digested BSA Waters, ACQUITY, and UPLC are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation. Comparative separations may not be representative of all applications. Phenomenex l WEB: 9
12 Kinetex.. More Resolution! Kinetex. Core-Shell Technology will show you what you re missing with conventional sub- fully porous columns. Waters ACQUITY BEH. C8 8 AU min AU min App ID 8 Kinetex. C8 AU More Resolution! min AU App ID min Conditions for all columns same except where noted: Column: Waters ACQUITY UPLC BEH. Gradient: Time (min) % B C8 0 Kinetex. C8.0 9 Dimensions: 0 x. mm. 9 Mobile Phase: A: 0. % Formic Acid in Water. B: 0. % Formic Acid in Acetonitrile Flow Rate: 0. ml/min Temperature: Ambient Detection: nm Instrument: Waters ACQUITY UPLC Sample:. Estriol. Hydrocortisone. Corticosterone. Cortisone acetate. -beta-estradiol. -alpha-estradiol. -OH-progesterone 8. -alpha-ethynylestradiol 9. Estrone 0. Deoxycorticosterone acetate. Progesterone Waters, ACQUITY, and UPLC are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Phenomenex is in no way affiliated with Waters Corporation. Comparative separations may not be representative of all applications. 0
13 UHPLC Column Protection R&D 00 Award Recipient Protect any UHPLC column Protect your column, including Kinetex core-shell columns, from damaging contaminants and microparticulates with the SecurityGuard ULTRA guard cartridge system! Simple to use Extend column lifetime Pressure rated to 0,000 psi (,8 bar) Fits virtually all manufacturers columns. to. mm ID UHPLC Column Protection SecurityGuard ULTRA Guard Cartridge System (Cartridge connected to Holder) Cartridge* Frit Holder Material Chamber Compounds of Interest } Contaminants and Particulates *Cartridge schematic not drawn to scale See it in action: Phenomenex l WEB:
14 Six selectivities. Take your pick. Choose from a wide range of phases for greater flexibility in UHPLC method development. Kinetex columns come in a variety of stationary phases to cover a full spectrum of applications ranging from acids and bases, to isomers and extremely polar compounds. Si C8 XB- C8 C8 Endcapped C8 phase Increased retention for polar basic compounds Protective isobutyl side chains Increased retention of polar acidic compounds Endcapped C8 phase Less hydrophobic than a C8 phase F ACN H 0 ACN ACN ACN ACN ACN ACN ACN F F ACN ACN ACN ACN H 0 ACN ACN ACN ACN H 0 H 0 H 0 F F H 0 HO O H 0 OH H 0 H 0 O H 0 Si H 0 OH Si HO Si H 0 HO OH Phenyl- Hexyl PFP HILIC Phenyl - Hexyl Greater retention and separation of aromatic hydrocarbons Pentafluorophenyl phase Unique aromatic and polar selectivity Unbonded silica phase Increased retention of polar compounds Material Characteristics Packing Material Pore Size (Å) Effective Surface Area (m /g) Effective Carbon Load % USP Classification ph Stability* Pressure Stability Kinetex C L. - 8.* 000 bar** Kinetex XB-C L. - 8.* 000 bar** Kinetex C L. - 8.* 000 bar** Kinetex PFP L. - 8.* 000 bar** Kinetex HILIC L bar** Kinetex Phenyl-Hexyl L. - 8.* 000 bar** *Columns are ph stable from.-0 under isocratic conditions. Columns are ph stable.-8. under gradient conditions. **Kinetex. columns of.0 and. mm ID are pressure rated to 00 bar.
15 Kinetex Ordering Information. Minibore Column (mm) Phases 0 x. C8 00B--AN SecurityGuard. μm Minibore Columns (mm) Ultra Cartridges Phases 0 x. 0 x. 00 x. 0 x. /pk XB-C8 00A-98-AN 00B-98-AN 00D-98-AN 00F-98-AN AJ0-88 C8 00A--AN 00B--AN 00D--AN 00F--AN AJ0-88 C8 00A-99-AN 00B-99-AN 00D-99-AN 00F-99-AN AJ0-88 PFP 00A--AN 00B--AN 00D--AN 00F--AN AJ0-88 HILIC 00A--AN 00B--AN 00D--AN AJ0-88 Phenyl-Hexyl 00B-00-AN 00D-00-AN 00F-00-AN AJ0-888 for. mm ID R&D 00 Award Recipient SecurityGuard. μm MidBore Columns (mm) Ultra Cartridges Phases 0 x.0 0 x.0 00 x.0 /pk XB-C8 00A-98-Y0 00B-98-Y0 00D-98-Y0 AJ0-8 C8 00B--Y0 00D--Y0 AJ0-8 C8 00A-99-Y0 00B-99-Y0 00D-99-Y0 AJ0-8 PFP 00B--Y0 00D--Y0 AJ0-880 HILIC 00B--Y0 AJ0-89 Phenyl-Hexyl AJ0-88 for.0 mm ID SecurityGuard. μm Minibore Columns (mm) Ultra Cartridges Phases 0 x. 0 x. x. 00 x. 0 x. /pk XB-C8 00A-9-AN 00B-9-AN 00C-9-AN 00D-9-AN 00F-9-AN AJ0-88 C8 00A--AN 00B--AN 00C--AN 00D--AN 00F--AN AJ0-88 C8 00A-9-AN 00B-9-AN 00C-9-AN 00D-9-AN 00F-9-AN AJ0-88 PFP 00A--AN 00B--AN 00C--AN 00D--AN 00F--AN AJ0-88 HILIC 00A--AN 00B--AN 00C--AN 00D--AN 00F--AN AJ0-88 Phenyl-Hexyl 00A-9-AN 00B-9-AN 00C-9-AN 00D-9-AN 00F-9-AN AJ0-888 for. mm ID SecurityGuard. μm MidBore Columns (mm) Ultra Cartridges Phases 0 x.0 0 x.0 x.0 00 x.0 0 x.0 /pk XB-C8 00A-9-Y0 00B-9-Y0 00C-9-Y0 00D-9-Y0 00F-9-Y0 AJ0-8 C8 00A--Y0 00B--Y0 00C--Y0 00D--Y0 00F--Y0 AJ0-8 C8 00A-9-Y0 00B-9-Y0 00C-9-Y0 00D-9-Y0 00F-9-Y0 AJ0-8 PFP 00A--Y0 00B--Y0 00C--Y0 00D--Y0 00F--Y0 AJ0-880 HILIC 00A--Y0 00F--Y0 AJ0-89 Phenyl-Hexyl 00D-9-Y0 00F-9-Y0 AJ0-88 for.0 mm ID SecurityGuard. μm Analytical Columns (mm) Ultra Cartridges Phases 0 x. 0 x. x. 00 x. 0 x. /pk XB-C8 00B-9-E0 00C-9-E0 00D-9-E0 00F-9-E0 AJ0-88 C8 00A--E0 00B--E0 00C--E0 00D--E0 00F--E0 AJ0-88 C8 00B-9-E0 00C-9-E0 00D-9-E0 00F-9-E0 AJ0-80 PFP 00A--E0 00B--E0 00C--E0 00D--E0 00F--E0 AJ0-8 HILIC 00B--E0 00C--E0 00D--E0 00F--E0 AJ0-8 Phenyl-Hexyl 00B-9-E0 00C-9-E0 00D-9-E0 00F-9-E0 AJ0-8 for. mm ID SecurityGuard ULTRA cartridges require holder, Part No. AJ Find Kinetex analytical and preparative dimensions online at: Phenomenex l WEB:
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