Chapter 3: Cells 3-1

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1 Chapter 3: Cells 3-1

2 Introduction: A. Human body consists of 75 trillion cells B. About 260 types of cells that vary in shape & size yet have much in common B. Differences in cell shape make different functions possible 3-2

3 A Composite Cell: A. includes many different cell structures B. main parts: nucleus, cytoplasm, cell membrane C. specialized organelles perform specific functions for the cell 3-3

4 3-4

5 D. Cell Membrane 1. regulates movement of substances in & out of the cell, participates in signal transduction, helps cells adhere to other cells 2. General Characteristics a. extremely thin & selectively permeable b. complex surface w/ adaptations to increase surface area DE9PK2w&list=UUGJqRYuHwar5 W6fzQO4VxxQ&index=10

6 3. Cell Membrane Structure: a. phospholipid bilayer, fatty acids tails turned inwards b. lipid soluble molecules can pass through c. embedded cholesterol molecules add strength & make the membrane less permeable to water-soluble substances d. many types of proteins are found in the cell membrane, including transmembrane & peripheral membrane proteins 3-6

7 e. Membrane proteins perform a variety of functions & vary in shape i. receptors on the cell surface ii. aid the passage across membrane iii. carbohydrates attached for cell ID iv. cellular adhesion molecules help determine cell interactions 3-7

8 3-8

9 E. Cytoplasm: 1. clear liquid = cytosol, a supportive cytoskeleton, networks of membranes & organelles a. Endoplasmic reticulum (ER)- made up of membranes, flattened sacs, & vesicles. Provides a tubular transport system inside the cell 3-9 i. Rough = has ribosomes,functions in protein synthesis & transport ii. Smooth = no ribosomes functions in lipid transport

10 3-10

11 b. Ribosomes - found w/ RER or scattered throughout the cytoplasm, composed of protein & RNA, provide structural support for the RNA molecules that come together in protein synthesis c. Golgi apparatus - composed of flattened sacs; refines, packages, modifies, & delivers proteins 3-11 i. Vesicles formed on ER travel to the Golgi which modifies their contents chemically ii. Vesicles then move to cell membrane & secrete its contents to the outside iii. Vesicles form a delivery service, carrying materials throughout the cell

12 d. Mitochondria - powerhouses of the cell, contain enzymes needed for aerobic respiration i. make ATP ii. more active cells have more mitochondria 3-12

13 e. Lysosomes - "garbage disposals" of the cell; contain digestive enzymes to break up old cell components & bacteria. f. Peroxisomes contain enzymes that function in the synthesis of bile acids, breakdown of lipids, degradation of rare biochemicals, and detoxification of alcohol. 3-13

14 g. Microfilaments & microtubules are thin, threadlike structures that serve as the cytoskeleton of the cell 3-14 i. Microfilaments: made of actin ii. Mictotubules: made of the globular protein tubulin h. Centrosome - made up of two hollow centrioles; function in the separation of chromosomes during cell division

15 i. Cilia & flagella - motile extensions from the cell; shorter cilia are abundant on the free surfaces of certain epithelial cells (ex:respiratory linings) cause movement of substances across the surface of the cell and a lengthy flagellum can be found on sperm cells j. Vesicles form part of the cell membrane or the Golgi/ER and store materials 3-15

16 F. Cell Nucleus: 1. bounded by a double-layered nuclear membrane w/ relatively large nuclear pores allow the passage of certain substances 3-16 that a. Nucleolus: in nucleus, made of RNA & protein; site of ribosome production, doesn t have a membrane b. Chromatin = loosely coiled fibers of protein & DNA

17 Movements Through Cell Membranes A. Passive: no energy 1. diffusion 2. facilitated diffusion 3. osmosis 4. filtration B. Active: cellular energy (ATP) 1. active transport 2. endocytosis exocytosis

18 C. Passive Mechanisms 1. Diffusion 3-18 a. caused by the random motion of molecules along the concentration gradient, from greater to lesser concentration until equilibrium is reached b. enables O 2 & CO 2 exchange btwn air & blood in the lungs, & btwn blood & tissue cells

19 2. Osmosis a. diffusion of water from greater water concentration (less osmotic pressure) to lower water concentration (greater osmotic pressure) b. Osmotic pressure is the ability of osmosis to lift a volume of water c. Compared to bodily fluids, a solution with i. the same osmotic pressure is isotonic ii. higher osmotic pressure is hypertonic iii. lower osmotic pressure is hypotonic 3-19

20 3. Facilitated Diffusion a. membrane proteins function as carriers to move molecules (glucose) across the cell membrane b. the # of carrier molecules in the cell membrane limits the rate of this process 3-20

21 3-21

22 3-22 SALT SUCKS

23 4. Filtration a. Because of hydrostatic pressure, molecules can be forced through membranes by the process of filtration. ex. Blood pressure in the kidneys 3-23

24 D. Active Mechanisms 1. Active Transport a. Uses ATP to move molecules from low to high concentration through transport proteins in cell membranes b. Up to 40% of a cell's energy may be used to fuel this process to get needed materials or eliminate wastes c. Actively transported substances include: sugars, amino acids, Na +, K +, Ca +2, H +, & nutrient molecules in the intestines 3-24

25 3-25

26 Comparing Facilitated Diffusion and Active Transport Transport of solutes across cell membranes by protein carriers can occur in one of two ways: The solute can move "downhill," from regions of higher to lower concentration, relying on the specificity of the protein carrier to pass through the membrane. This process is called passive transport or facilitated diffusion, and does not require energy. The solute can move "uphill," from regions of lower to higher concentration. This process is called active transport, and requires some form of chemical energy. The transport process a cell uses depends on its specific needs. For example, red blood cells rely on facilitated diffusion to move glucose across membranes, whereas intestinal epithelial cells use active transport to take in glucose from the gut. Facilitated diffusion is effective for red blood cells because the concentration of glucose in the blood is stable and higher than the cellular concentration. On the other hand, active transport is needed in the gut because there are large fluctuations of glucose concentration as a result of eating. 3-26

27 2. Endocytosis & Exocytosis a. Endocytosis - molecules too large to be transported by other means are engulfed by an invagination of the cell membrane & carried into the cell surrounded by a vesicle b. Exocytosis is the reverse of endocytosis c. 2 forms of endocytosis i. Pinocytosis - cells engulf liquids 3-27 ii. Phagocytosis - cells engulf large particles (WBC eat bacteria)

28 3-28

29 3-29

30 The Cell Cycle A. series of changes a cell undergoes from the time it is formed until it reproduces B. interphase, mitosis, cytokinesis, and differentiation C. Highly regulated, most cells do not divide continually. Cells have a maximum number of times they can divide because of built-in clocks (telomeres) on the tips of chromosomes 3-30

31 Cell Cycle m/watch?feature=player _embedded&v=vln7k 1-9QB

32 E. Mitosis/Meiosis 1. Meiosis, four cells, gametes (sperm/ova) are produced, each of which contains half of the parent cell s genetic information 2. Mitosis is a carefully orchestrated division of the nucleus of the cell that results in 2 daughter cells receiving an exact copy of the mother cell's genetic material 3-32

33 D. Interphase 1. a period of great metabolic activity in which the cell grows & synthesizes new molecules and organelles 2. S phase: the DNA is replicated in preparation for cell division 3. G 1 & G 2 phases: of interphase, the cell grows & other structures are duplicated 3-33

34 3. Mitosis is described as a series of 4 stages, but the process is continuous 4. Prophase: DNA condenses into chromosomes, centrioles migrate to the poles, microtubules of the cytoskeleton reorganize into spindle fibers, & the nuclear envelope disappears 3-34

35 5. Metaphase: spindle fibers attach to centromeres on the chromosomes & chromosomes align midway btwn centrioles 6. Anaphase: spindle fibers contract & pull sister chromatids apart 7. Telophase: chromosomes have completed their migrations making 2 identical groups, nuclear envelopes reappear, chromosomes begin to unwind 3-35

36 3-36

37 F. Cytoplasmic Division 1. Cytokinesis begins during anaphase of mitosis & continues as a contractile ring pinches the two new cells apart 2. The two daughter cells may have varying amounts of cytoplasm & organelles, but they share identical genetic info. 3-37

38 G. Cell Differentiation 1. Process by which cells develop into different types of cells with specialized functions 2. reflects genetic control of the nucleus as certain genes are turned on while others are turned off 3-38

39 3-39

40 H. Cell Death 1. Apoptosis is a form of cell death that is a normal part of development Cancer uncontrolled cell division Metastasis cancer spreads 3-40

41 Stem Cells

42 Animations/ Links UUGJqRYuHwar5W6fzQO4VxxQ (scroll down for animations)

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