RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS DETERMINATION OF CITICOLINE AND PIRACETAM IN PHARMACEUTICAL DOSAGE FORM
|
|
- Coleen Miles
- 6 years ago
- Views:
Transcription
1 Page3639 Indo American Journal of Pharmaceutical Research, 2015 ISSN NO: RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS DETERMINATION OF CITICOLINE AND PIRACETAM IN PHARMACEUTICAL DOSAGE FORM Manutosh Acharya 1, A.K Jain 2, Naveen Sharma 3, Suman Jain 4, Gurdeep Singh Saluja 5, Ashish Dixit 6 1 OmniActive Health Technologies, Thane (W) Pharmaceutical Chemistry, Department of Pharmacology, Gajraraja Medical College, Gwalior (M.P. 3 Department of Pharmacology, Shri Ram Pharmacy College, Gwalior (M.P.) 4 SOS Pharmaceutical Sciences, Jiwaji University, Gwalior (M.P) 5 Department of Pharmaceutical Chemistry, Shri Ram Pharmacy College, Gwalior (M.P.) 6 Department of Pharmaceutical Analysis, Shri Ramnath Singh Institute of Pharmaceutical Science and Technology, Gwalior (M.P.) ARTICLE INFO Article history Received 17/08/2015 Available online 10/09/2015 Keywords, Citicoline, Assay, RP-HPLC. Corresponding author Manutosh Acharya Vice President-R&D OmniActive Health Technologies New Technology Centre, A-10 Road No 1, Wagle Industrial Estate, Thane (W) , India m.acharya@omniactives.com ABSTRACT A new simple, selective, rapid, precise reversed phase high performance liquid chromatography method has been developed and validated for the simultaneous estimation of and Citicoline in a pharmaceutical dosage form. The separation was made using Inertsil C18, (250 x 4.6) mm, 5µm column. Mobile phase used contained Phosphate buffer and acetonitrile in gradient mode at wavelength of 210nm. The mobile-phase flow rate and the sample volume injected were 1 ml/min and 10 μl, respectively. Retention time of Citicoline and were found to be 4.2 ±0.2mins, 12.3±0.2minsrespectively. A good linear relationship (Citicoline r=0.999& r=0.999) was observed over a concentration range of to µg/ml of and to µg/ml of Citicoline. The limit of detection (LOD) and limit of quantification (LOQ) for Citicoline was found to be 0.08 ppm&0.26ppm and for it was found to be0.12 ppm & 0.42ppm. It was concluded that in the present developed RP- HPLC method, the standard and sample preparation required less time and without tedious extraction. The developed method is simple, rapid, and accurate, hence can be used for routine quality control analysis in Pharmaceutical industry. The method was optimized on gradient mode; offering better resolution of peak of interest as well as impurities in contrast to all existing methods which are on isocratic mode. Please cite this article in press as Manutosh Acharya et al. RP-HPLC Method Development and Validation For Simultaneous Determination of Citicoline and in Pharmaceutical Dosage Form. Indo American Journal of Pharmaceutical Research.2015:5(08). Copy right 2015 This is an Open Access article distributed under the terms of the Indo American journal of Pharmaceutical Research, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
2 Page3640 INTRODUCTION Citicoline (Cytidine-5 -diphosphocholine or CDP) is an intermediate in the biosynthesis of phosphatidylcholine.it play important roles in cellular metabolism. Citicoline is readily absorbed in the GIT and widely distributed throughout the body, crosses the Central Nervous system, where it is incorporated into the membrane and microsoma fraction. It activates biosynthesis of structural phospholipids, increases brain metabolism, and acts on the levels of different neurotransmitters. It has a variety of applications in CNS injury models neurological disorders of the brain such as stroke, brain, trauma, Alzheimer's and Parkinson s disease. (1,2,3) is a nootropic drug in the racetams group, with chemical name 2-oxo-1-pyrrolidine acetamide. is a cyclic derivative of GABA. Both drugs are psychotherapeutic agents, used as psycho stimulant, nontropic and neurotonics.both drugs are freely soluble in water. These drugswill increase cerebral metabolism and increase level of various neurotransmitters, including acetylcholine and dopamine, exerting its action by activating the biosynthesis of structural phospholipids in neuronal membrane. This drug will increase the blood flow and oxygen consumption in brain. (1, 2, 3) The review of literature regarding quantitative analysis of Citicoline and revealed that the attempts were made to develop analytical methods for estimaton of Citicoline and in serum. Some spectrometric methods and LC methods have been reported for the estimation of the individual drugs. The focus of the present study was to develop, optimize and validate a rapid, specific, and economic and stability indicating RP-HPLC method for the simultaneous estimation of Citicoline and in tablet dosage form adaptable by pharmaceutical industry. (4,5,6) The method was optimized on gradient mode ;offering better resolution of peak of interest as well as impurities so it is useful even for studies degradation impurities. MATERIALS AND METHODS Materials HPLC:Waters HPLC series consisting pump, Auto sampler, UV- Visible detector, Thermostat column compartment connected with Waters (alliance) Empower2 software Balance Sartorius Cpa225d Semi Micro Balance. Sonicator Bio-Technics India, Mumbai Reagents buffer reagents such as potassium dihydrogen phosphate, ion pairing reagents such as hexane sulphonic acid sodium salt. Organic solvents such as methanol and acetonitrile Methodology-Assay and Validation of Simultaneous determination of and Citicoline in tablet Chemicals and Reagents Potassium dihydrogen orthophosphate, hexane sulphonic acid sodium salt, ortho phosphoric acid Chromatographic conditions Column : Inertsil C18, (250 x 4.6) mm, 5µm or equivalent Detector : UV-Visible (Use HPLC with PDA, MWD or DWD) Detection Wavelength : 210 nm Flow rate : 1.0 ml/min Injection volume : 10 µl Column oven temperature : 30 C Sample cooler temperature : NA Runtime : 25minutes Diluent : N HCl (ph 3.0 HCl) Table 1:Gradient Program. Time (Minutes) Mobile Phase A Mobile Phase B i)blank: N HCl (ph 3.0 HCl) ii)mobile Phase Mobile Phase - A: 6.8 gm of potassium dihydrogen orthophosphate & 1.0 gm of hexane sulphonic acid sodium salt were dissolved in 1000 ml Mili-Q water, ph of above solution was adjusted to 3.0 ± 0.05 with diluted ortho phosphoric acid.themobile phase buffer was filtered through 0.45 µm membrane filter. Mobile Phase - B: Acetonitrile Preparation of Diluent: N HCl
3 Page3641 iii) Standard Preparation: 40 mg of working standard and 27.2 mg of Citicoline sodium working standard (eq.to 25 mg of Citicoline) was weighed accurately into a clean and dry 100 ml volumetric flask, 50 ml of diluent was added and sonicated to dissolve. Volume was make-up to the mark with diluent.(concentration of Citicoline was about 250µg/ml and was about 400µg/ml). iv) Sample Preparation: 20 tablets were weighed accurately and the average weight was determined. The tablets were crushed to fine powder. The powder equivalent to 500 mg of Citicoline were weighed into a 200 ml volumetric flask, 100 ml of Diluent was added to it and sonicated for 15 minutes with intermittent shaking. The solution was allowed to cool at room temperature. Volume was make-up to the mark with diluent, mixed & Filtered. 5 ml of this stock solution was pipetted out in 50 ml volumetric flask and diluted up to the mark with diluent.(concentration of Citicoline was about 250µg/ml and was about 400µg/ml). The order of injection followed in HPLC was: Table 2:Order Of Injection. Sr.No. Sample Name No. of Injections 1 Blank 1 2 Standard preparation 5 3 Test preparation 2 Validation of quantitative HPLC method (7, 8): The optimized RP-HPLC method for assay was validated according to the procedures described in ICH guidelines Q2 (R1) for the validation of analytical method (ICH 2005). Specificity Specificity is the ability to assess unequivocally the analyte in the presence of components which may be expected to be present. Typically these might include impurities, degradants, matrix, etc Linearity & Range It is the ability of the method to elicit test result that is directly proportional to analyte concentration within a given range. Acceptance criteria: The correlation coefficient should be NLT Calibration curve was plotted over a concentration range of to µg/ml of Citicoline and to µg/ml of. Samples are prepared, filtered and injected into HPLC system. Aliquots (10 µl) of each solution were injected from auto sampler under the operating chromatographic conditions described above. Calibration Curve was constructed by plotting peak area verses concentration and the regression equation was calculated. Accuracy& Recovery The absolute recovery of analytical method was measured as the response of a processed spiked matrix standard expressed as a percentage of the response of pure standard which has not been subjected to sample pretreatment and indicates whether the method provides a response for the entire amount of analyte that was present in the sample. The accuracy of the method was determined by calculating recoveries of Citicoline and by the standard addition method. Known amounts of standard solutions of Citicoline and were spiked to placebo preparation at different levels (40%, 80%, 100% and 120% levels). The area under curve obtained was checked and analyzed for the recovery percentage. Limit of Detection (LOD) and Limit of Quantification (LOQ) The limit of detection (LOD) and the limit of quantification (LOQ) of Citicoline and were derived by Calculating the signal-to-noise ratio (S/N) using the following equations designated by International Conference on Harmonization (ICH) guidelines. LOD = 3.3 s/s LOQ = 10 s/s Where, s = the standard deviation of the response and S = slope of the calibration curve. The LOD and LOQ were estimated by injecting lower concentration solutions of Citicoline and and determining % RSD of area responses of 6 replicate injections. The LOD and LOQ were confirmed and recorded separately on the basis of signal (S) to noise (N) ratio.
4 Page3642 Method Precision The precision of method was checked and verified by repeatability,inter-day precision and variability due to analyst.repeatability was checked by injecting six test preparations into the HPLC system as per the test method.ruggedness of the proposed analytical method was evaluated for variability studies like, Variability due to Analyst and variability due to different day. Assay of Citicoline sodium & tablets was performed in six replicates as per the proposed test method and analysedfor proposed variability. Results were compared with the method precision data obtained under precision studies. The % Assay was calculated for each of the sample. Solution Stability The solution stability of Citicoline and sample and standard solution was carried out by leaving both the test and standard solution in tightly capped volumetric flask at room temperature for 15 hours. The same sample solution was analyzed initially and at various time intervals up to the 15 hours throughout the study period. Robustness To determine the robustness of the developed method, minute changes were made in the flow rate, percentage of organic phase, mobile phase ph and column compartment temperature. The deviations in all parameters from optimized method were studied. System Precision System suitability experiments can be defined as tests to ensure that the system can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method development and validation have been completed. To perform the system suitability test the standard solution were freshly prepared and injected under the condition of optimized method. Table 3: Validation Parameters &their Acceptance criteria. S.No. Parameters Acceptance criteria 1 Specificity No interference observed for response due to analyte or impurities of interest 2 Linearity & Range correlation coefficient NLT Accuracy & Recovery NLT 98.0% and NMT 102.0% at various recovery levels 4 Limit of Detection (LOD) To be determined by test 5 Limit of Quantification (LOQ) To be determined by test 6 Method Precision RSD NMT 2.0% 7 Solution Stability To be determined by test 9 System Precision RSD NMT 2.0% RESULTS AND DISCUSSION Optimised method obtained was using Phosphate buffer (6.8 gm of potassium dihydrogen orthophosphate & 1.0 gm of hexane sulphonic acid sodium salt in 1000 ml Mili-Q water, ph 3.0±0.1 adjusted with diluted ortho phosphoric acid) and acetonitrile under gradient mode as Mobile phase and a flow rate of 1.0 ml/min. Peaks were well defined, symmetrical and resolved. Retention time of Citicoline and was found to be 4.2 ±0.2, 12.3±0.2 mins respectively and the optimum wavelength was determined to be 210 nm. Specificity Blank (diluent), placebo and samples were injected into HPLC and peak Purity of analyte Peaks and impurity peaks was checked. The impurity peaks were found to be well resolved from each other and from analytes peaks. No interference was observed at the retention time of analyte peak from the blank & placebo solution. Linearity & Range: The linearity data for and Citicoline has been given in Table 4.
5 Page3643 Table 4: Linearity data for. Citicoline S. No. Conc. Conc. (µg/ml ) (µg/ml ) Slope Intercept CC The correlation coefficient was observed to be and for and Citicoline respectively that meets the acceptance criteria (NLT 0.999). This concludes that the method is linear throughout the range selected. Limit of Detection (LOD) and Limit of Quantification (LOQ): The LOD and LOQ were estimated by injecting lower concentration solutions of Citicoline and and determining % RSD of area responses of 6 replicate injections. Data obtained is shown in Table-5. Table-5: LOD and LOQ data of. Concentration ppm % w/w LOD LOQ Injection no. Counts -LOD S/N Value Counts - LOQ S/N Value Mean SD RSD (%) Table-6: LOD and LOQ data of Citicoline. Injection no. Counts -LOD S/N Value Counts - LOQ S/N Value Mean SD RSD (%) The % RSD for both the analytes was found well within the accepted criteria (NMT 10.0% at LOQ level and NMT 33.0% at LOD level). Hence the method has suitable level of LOD/LOQ. Accuracy: Data obtained shown in Table 7.
6 Page3644 Table 7: Accuracy (% Recovery) data for and Citicoline. Recovery Level % Recovery for % Recovery for Citicoline 40% level % Level % Level % Level The % Recovery was found well within the acceptance criteria at various recovery levels (NLT 98.0% and NMT 102.0%). Hence the method is accurate throughout the selected range. Solution Stability: The data shown in Table 8 indicate that the sample solution is stable for 13 hours at room temperature. Timepoint Table-8: Solution Stability data of Citicoline& at room temperature. Standard Preparation Sample Preparation Citicoline Citicoline Absolute % Absolute % Absolute % Time- Difference Difference Difference Counts Counts point Counts Counts w.r.t. Initial w.r.t. Initial w.r.t. Initial Initial Initial hr hr hr hr hr hr hr hr hr hr hr hr hr hr Absolute % Difference w.r.t. Initial From the results it is concluded that the standard solution is stable for about 15 hours and sample solution is stable for about 13 hours at room temperature. Robustness: System suitability parameters were met under all robustness conditions except change in organic content in gradient program where significant retention time shift was observed for both the analyses peaks. Hence it is recommended that organic content should be strictly adhered to as per the method. The system suitability data under robustness conditions has been summarized in the Table 9.
7 Page3645 Table 9: Robustness Data for Standard solution. Condition Standard Preparation Citicoline RT USP Plate Count USP Tailing RT USP Plate Count USP Tailing Control Flow Minus (0.9 ml/min) Flow Plus (1.1 ml/min) Temperature Minus (25 C) Temperature Plus (35 C) Control (-) Organic content in Mobile Phase B in gradient program* (+) Organic content in Mobile Phase B in gradient program* ph Minus (ph=2.80) ph Plus (ph=3.20) Wavelength minus (λ=205) Wavelength plus (λ=215) The retention time (RT) and system suitability data under various robustness conditions in comparison to control sample has been summarized in the Table 10. Table 10: Robustness Data for Control Sample. Control Sample Condition Citicoline RT USP Plate Count USP Tailing RT USP Plate Count USP Tailing Control Flow Minus (0.9 ml/min) Flow Plus (1.1 ml/min) Temperature Minus (25 C) Temperature Plus (35 C) Control (-) Organic content in Mobile Phase B in gradient program (+) Organic content in Mobile Phase B in gradient program ph Minus (ph=2.80) ph Plus (ph=3.20) Wavelength minus (λ=205) Wavelength plus (λ=215)
8 Page3646 Parameters Linearity And Range Regression Line Equation Correlation Coefficient (R 2 ) Precision (%RSD) Method Precision (n=6) Intermediate Table 11: Summary Of Validation Parameters For Assay. Acceptance Criteria Correlation Coefficient(R 2 ) NLT NMT 2.0 NMT 2.0 Citicoline µg/ml y=12108x µg/ml y=12450x Ruggedness (n=12) RSD 2.0% LOD (n=6) 0.08µg/mL 0.12µg/mL (0.01%w/w) (0.01%w/w) LOQ (n=6) 0.26µg/ml 0.42µg/ml (0.04%w/w) (0.04%w/w) Accuracy (98.0% %) 1. 40% level 2. 80% Level % Level % Level Solution Stability (At Room Temperature) Specificity Robustness 2% RSD Complies 2% RSD for 13 Hours Complies 2% RSD for 13 Hours Specific (Passes peak purity data analysis) Robust except Organic Plus condition CONCLUSION A new simple, selective, rapid, precise reversed phase high performance liquid chromatography method has been developed and validated for the simultaneous estimation of and Citicoline in a pharmaceutical dosage form. The method is suitable for routine QC analysis in Pharmaceutical industry. The method was optimized on gradient mode and is capable of resolving peaks between main peaks and any impurities &/or degradants formed during stability and hence can be used as stability indicating method (work to be published subsequently).the similar chromatography method may be applied to related substances also. Future research is recommended in any other pharmaceutical dosage form as the method is optimized to elute the maximum numbers of impurities. CONFLICT OF INTEREST The authors confirm that there is no conflict of interest. ACKNOWLEDMENT The author is thankful to the management and colleagues of Plethico Pharmaceuticals Limited and Jiwaji University for providing the research facilities, drugs and initiatives to carry out the work. The author is thankful to Ratna Upadhyay for supporting in research publication.
9 Page3647 REFERENCES 1. Wurtman RJ et al., Effect of oral CDP- choline on plasma choline and uridine levels in humans,biochempharmacol, 2000, 60 (7), Rao AM et al., CDP- choline: neuroprotection in transient forebrain ischemia of gerbils." Journal ofneuroscience Res, 1999, 58(5), Neil MJ, editor. The Merck Index- and Encyclopedia of chemicals. 13 th Ed. New Jercydrugs and biological; Merck and Co; 2001; 388 and RaveendraB.Ganduri, et al.,stability indicating LC method for the determination of citicoline sodium in injection formulation, International Journal of Pharm Tech Research, Vol.2, No.1, Page no , (Jan-Mar 2010). 5. Sonali O. Uttarwar, et al., Stability indicating LC Method for Citicoline Sustained Release Tablet,International Journal of PharmTech Research, Vol.2, No.4, pp , (Oct-Dec 2010) 6. Robert N. Nalbandlan, et al., Liquid-Chromatographic Quantification of, CLINICAL CHEM., Vol. 29, No. 4,page no (1983) 7. ICH guidelines, Q1 A stability testing of new drug substances and products, ICH guidelines, Q2 B Analytical procedure, Methodology,
Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin in Pharmaceutical Dosage Form using RP-HPLC Method
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.4, pp 1736-1744, Oct-Dec 2013 Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin
More informationMETHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR ESTIMATION OF ZOLPIDEM TARTARATE
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Ramalakshmi et al. SJIF Impact Factor 6.647 Volume 7, Issue 2, 1010-1018 Research Article ISSN 2278 4357 METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(1):6-10 ISSN: 0976-8688 CODEN (USA): PSHIBD Validated RP-HPLC method for simultaneous estimation of metformin hydrochloride
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(9): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(9):70-80 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of Stability Indicating
More informationDevelopment and validation of stability indicating RP-LC method for estimation of calcium dobesilate in pharmaceutical formulations
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (11):236-242 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationHyderabad, India. Department of Pharmaceutical Chemistry, Glocal University, Saharanpur, India.
International Journal On Engineering Technology and Sciences IJETS RP-HPLC Method development and validation for the Simultaneous Estimation of Metformin and Empagliflozine in Tablet Dosage Form Shaik
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(5):91-98 ISSN: 0976-8688 CODEN (USA): PSHIBD A novel RP-HPLC method development and validation of Perindopril Erbumine in
More informationCHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG
CHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE IN TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG ANALYTICAL METHOD VALIDATION REPORT FOR ASSAY 43 2.1 Introduction Analytical
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
51 CHAPTER 2 SIMULTANEOUS ESTIMATION OF PIOGLITAZONE, GLIMEPIRIDE AND GLIMEPIRIDE IMPURITIES IN COMBINATION DRUG PRODUCT BY A VALIDATED STABILITY-INDICATING RP-HPLC METHOD 2.1 INTRODUCTION OF DOSAGE FORM
More informationInternational Journal of Pharma and Bio Sciences DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE ANALYTICAL CHEMISTRY DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET K.MYTHILI *, S.GAYATRI,
More informationDevelopment and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in pure and tablet dosage form
ISSN: 2320-2831 IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: Research article Open Access Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in
More informationSimultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by RP-HPLC method
International Journal of Chemical and Pharmaceutical Sciences 2017, Mar., Vol. 8 (1) ISSN: 0976-9390 IJCPS Simultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by
More informationRP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BACLOFEN IN BULK AND PHARMACEUTICAL DOSAGE FORMS
Int. J. Chem. Sci.: 11(1), 2013, 390-398 ISSN 0972-768X www.sadgurupublications.com RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BACLOFEN IN BULK AND PHARMACEUTICAL DOSAGE FORMS SAROJ
More informationA simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical dosage form
IJPAR Vol.4 Issue 1 Jan-Mar-2015 Journal Home page: ISSN: 2320-2831 Research article Open Access A simple validated RP-HPLC method for quantification of sumatriptan succinate in bulk and pharmaceutical
More informationRP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form
RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form S. LAVANYA* 1, SK. MANSURA BEGUM 1, K. NAGAMALLESWARA RAO 2, K. GAYATHRI DEVI 3 Department of pharmaceutical
More informationInternational Journal of Pharma and Bio Sciences
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE PHARMACEUTICAL ANALYSIS DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR ESTIMATION OF ESCITALOPRAM OXALATE IN TABLET DOSAGE
More informationA New Stability-Indicating and Validated RP-HPLC Method for the Estimation of Liraglutide in Bulk and Pharmaceutical Dosage Forms
OPEN ACCESS Eurasian Journal of Analytical Chemistry ISSN: 1306-3057 2017 12(2):31-44 DOI 10.12973/ejac.2017.00152a A New Stability-Indicating and Validated RP-HPLC Method for the Estimation of Liraglutide
More informationDevelopment and validation of related substances method for Varenicline and its impurities
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (1):304-309 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationDevelopment and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets
Journal of PharmaSciTech 0; ():- Research Article Development and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets * Sayyed Hussain,
More informationDevelopment of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method
Research Article Development of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method * S. M. Sandhya, G. Jyothisree, G. Babu Department
More informationJournal of Chemical and Pharmaceutical Research, 2013, 5(1): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(1):180-184 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 A simple and sensitive RP-HPLC method for estimation
More informationJ Pharm Sci Bioscientific Res (4): ISSN NO
Development and Validation of Analytical Methods for Simultaneous Estimation of Pregabalin and Amitriptyline Hydrochloride in their Combined Marketed Dosage form ABSTRACT: Nikhilkumar Patel, Gurjit Kaur,
More informationRP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms
Asian Journal of Chemistry Vol. 22, No. 7 (2010), 5067-5071 RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms A. LAKSHMANA RAO*, G. TARAKA RAMESH and J.V.L.N.S. RAO Department of Pharmaceutical
More informationA stability indicating RP-HPLC method for simultaneous estimation of darunavir and cobicistat in bulk and tablet dosage form
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (12):89-96 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationIJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page:
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Method development and validation of tenofovir disoproxil fumerate and emtricitabine in combined tablet
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF LACOSAMIDE IN BULK AND ITS PHARMACEUTICAL FORMULATION
http://www.rasayanjournal.com Vol.4, No.3 (2011), 666-672 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR IN BULK AND ITS PHARMACEUTICAL FORMULATION V.Kalyan Chakravarthy*
More informationAiro International Research Journal ISSN : Volume : 7 October 2015
Airo International Research Journal ISSN : 2320-3714 Volume : 7 October 2015 METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF ESCITALOPRAM OXALATE AND ETIZOLAM IN TABLET DOSAGE FORM
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF GEFITINIB
More informationEstimation of Zanamivir Drug present in Tablets using RP-HPLC Method
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol. 3, No.1, pp 180-186, Jan-Mar 2011 Estimation of Zanamivir Drug present in Tablets using RP-HPLC Method Ravindra Reddy.Y*,
More informationISSN (Print)
Scholars Academic Journal of Pharmacy (SAJP) Sch. Acad. J. Pharm., 2014; 3(3): 240-245 Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources) www.saspublisher.com
More informationNOVEL RP-HPLC METHOD. B.Lakshmi et a. concentration range KEY INTRODUCTION. Diltiazem is used to. . It works by of contractionn of the. (dilate).
B.Lakshmi et a ISSN-2319-2119 al, The Experiment, January. 2013 Vol..6(4), 365-371 A NOVEL RP-HPLC METHOD FOR THE DETERMINATION OF DILTIAZEM IN PHARMACEUTICAL DRUG PRODUCTS ABSTRACT High resolution RP-HPLC
More informationScholars Research Library. Der Pharmacia Lettre, 2016, 8 (6): (http://scholarsresearchlibrary.com/archive.html)
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (6):217-223 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationAMERICAN JOURNAL OF BIOLOGICAL AND PHARMACEUTICAL RESEARCH
AMERICAN JOURNAL OF BIOLOGICAL AND PHARMACEUTICAL RESEARCH e-issn - 2348-2184 Print ISSN - 2348-2176 Journal homepage: www.mcmed.us/journal/ajbpr VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 11(4), 2013, 1607-1614 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS
More information36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014
36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014 Original Research Article SIMPLE AND RAPID LIQUID CHROMATOGRAPHIC METHOD FOR REAL-TIME QUANTIFICATION OF NAPROXEN / ESOMEPRAZOLE MAGNESIUM
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(1):138-144 Simultaneous RP HPLC determination of Latanoprost
More informationRP- HPLC and Visible Spectrophotometric methods for the Estimation of Meropenem in Pure and Pharmaceutical Formulations
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol. 3, No.2, pp 605-609, April-June 2011 RP- HPLC and Visible Spectrophotometric methods for the Estimation of Meropenem
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com VALIDATED RP-HPLC METHOD FOR DETERMINATION OF BROMHEXINE
More informationResearch Article Simultaneous Estimation of DL-Methionine and Pyridoxine Hydrochloride in Tablet Dosage Form by RP-HPLC
Research Article Simultaneous Estimation of DL-Methionine and Pyridoxine Hydrochloride in Tablet Dosage Form by RP-HPLC Shinde Prashanti 1 *, Mane Aruna 2, Palled Mahesh 1, Bhat AR 1 and Karagane Swapna
More informationDevelopment, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method
ISSN: 0973-4945; CODEN ECJAO E- Chemistry http://www.e-journals.net 2012, 9(1), 340-344 Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by PLC Method V.
More informationRao and Gowrisankar: Stability-indicating RP-HPLC Method for Pseudoephedrine, Ambroxol and Desloratadine
Research Paper Development and Validation of Stability-indicating RP- HPLC Method for the Estimation of Pseudoephedrine, Ambroxol and Desloratadine in Bulk and Tablet Dosage Forms N. MALLIKARJUNA RAO*
More informationANALYTICAL TECHNIQUES FOR THE QUALITY OF ANTI- AIDS DRUGS
Research Article N. Bala Krishna, IJPRBS, 2013; Volume 2(1): 63-77 ISSN: 2277-8713 IJPRBS N.BALAA KRISHNA, Dr. M.V.V NAGESWARA REDDY IJPRBS-QR CODE PAPER-QR CODE DEVELOPMENT OF NEW ANALYTICAL TECHNIQUES
More informationInternational Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:
Research Article CODEX: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD ESTIMATION OF TOLVAPTAN IN BULK PHARMACEUTICAL FORMULATION
http://www.rasayanjournal.com Vol.4, No.1 (2011), 165-171 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR AND ITS PHARMACEUTICAL FORMULATION V. Kalyana Chakravarthy * and
More informationAnalytical Method Development and Validation for the Estimation of Guaifenesin and Dextromethorphan by RP-HPLC
Human Journals Research Article September 2018 Vol.:13, Issue:2 All rights are reserved by Ramya Sree Borra et al. Analytical Method Development and Validation for the Estimation of Guaifenesin and Dextromethorphan
More informationIsocratic Reversed Phase Liquid Chromatographic Method Validation for the Determination of Cilostazol in Pure and Formulations
Human Journals Research Article October 2015 Vol.:4, Issue:3 All rights are reserved by Rambabu K et al. Isocratic Reversed Phase Liquid Chromatographic Method Validation for the Determination of Cilostazol
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):770-775 Validation of Rapid Liquid Chromatographic
More informationJournal of Pharmacreations
Journal of Pharmacreations ISSN: 2348-6295 Pharmacreations Vol.5 Issue 1 Jan- Mar- 2018 Journal Home page: www.pharmacreations.com Research article Open Access Method development and validation of apixaban
More informationMethod Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC
Original Research Method Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC Hanumantha Rao K 1, Lakshmana Rao A 2,*, Chandra Sekhar KB 3 1 Assistant
More informationF. Al-Rimawi* Faculty of Science and Technology, Al-Quds University, P.O. Box 20002, East Jerusalem. Abstract
JJC Jordan Journal of Chemistry Vol. 4 No.4, 2009, pp. 357-365 Development and Validation of Analytical Method for Fluconazole and Fluconazole Related Compounds (A, B, and C) in Capsule Formulations by
More informationWorld Journal of Pharmaceutical Research
World Journal of Pharmaceutical ReseaRch Volume 3, Issue 3, 4527-4535. Research Article ISSN 2277 715 DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPLC METHOD FOR ESTIMATION OF RAMOSETRON Zarana
More informationVALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DEXAMETHASONE AND GRANISETRON IN COMBINED DOSAGE FORMS
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DEXAMETHASONE AND GRANISETRON IN COMBINED
More informationSimultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage Form by RP-HPLC Method
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1212-1217 Simultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage
More informationNew RP-HPLC Method for the Determination of Fludarabine in Pharmaceutical Dosage Forms
Online : ISSN 2349-669X Print : ISSN 0973-9874 New RP-HPLC Method for the Determination of Fludarabine in Pharmaceutical Dosage Forms LAKSHMI B 1, *, RAMA KRISHNA K 2 AND JAYAVEERA K N 3 1. Department
More informationTentu Nageswara Rao et al. / Int. Res J Pharm. App Sci., 2012; 2(4): 35-40
International Research Journal of Pharmaceutical and Applied Sciences Available online at www.irjpas.com Int. Res J Pharm. App Sci., 2012; 2(4):35-40 Research Article Estimation of Fesoterodine fumarate
More informationCorresponding Author:
Adv J Pharm Life sci Res, 2017 5;3:1-8 ISSN 2454 3535 (On-line) RP-HPLC Method for Estimation of Mupirocin in Bulk and Pharmaceutical Formulation S.K.Attar 1 *, M.S.Kalshetti 2, N. A. Jadhao 3, N. R. Patel
More informationSujatha and Pavani et.al. Indian Journal of Research in Pharmacy and Biotechnology ISSN: (Print) ISSN: (Online)
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF AMITRIPTYLINE HYDROCHLORIDE AND CHLORDIAZEPOXIDE IN TABLET BY RP-HPLC Neeli Sujatha* K Haritha Pavani Department of Pharmaceutical Analysis and Quality Assurance,
More informationVolume 2 (6), 2014, Page CODEN (USA)-IJPRUR, e-issn: International Journal of Pharma Research and Health Sciences
CODEN (USA)-IJPRUR, e-issn: 2348-6465 International Journal of Pharma Research and Health Sciences Available online at www.pharmahealthsciences.net Original Article Simultaneous Determination of Salbutamol
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF TRAMADOL IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 10(4), 2012, 2039-2047 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS OF TRAMADOL IN PURE AND PHARMACEUTICAL FORMULATIONS
More information10 Kadam V. N. et al. Ijppr.Human, 2014; Vol. 1(2): 10-21 (Research Article) DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS FOR SIMULTANEOUS ESTIMATION OF VOGLIBOSE, GLIMEPIRIDE AND METFORMIN HYDROCHLORIDE
More informationStability indicating RP-HPLC method development and validation of Etizolam and Propranolol hydrochloride in pharmaceutical dosage form
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationPankti M. Shah et al, Asian Journal of Pharmaceutical Technology & Innovation, 04 (17); 2016; 07-16
Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 30-03-2016 Accepted on: 01-04-2016 Published on: 15-04-2016 Corresponding Author: *Pankti M. Shah,
More informationJournal of Chemical and Pharmaceutical Research, 2018, 10(3): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2018, 10(3):142-147 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development of Reverse Phase HPLC Method and Validation
More informationAnkit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), Available online at RESEARCH ARTICLE
Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), 26-30 26 Available online at http://jddtonline.info RESEARCH ARTICLE METHOD DEVELOPMENT AND ITS VALIDATION FOR QUANTITATIVE SIMULTANEOUS
More informationSIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD
170 Original Article SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD *Lakshmana Rao A, 1 Bhaskara Raju V *V.V. Institute of Pharmaceutical Sciences, Gudlavalleru,
More informationComparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation
Oriental Journal of Chemistry Vol. 24(2), 537-544 (2008) Comparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation P.N. DALVI,
More informationDETERMINATION OF LOTEPREDNOL ETABONATE AND TOBRAMYCIN IN COMBINED DOSAGE FORM USING RP-HPLC METHOD
280 P a g e e-issn: 2248-9126 Vol 5 Issue 4 2015 280-284. Print ISSN: 2248-9118 Indian Journal of Pharmaceutical Science & Research www.ijpsrjournal.com DETERMINATION OF LOTEPREDNOL ETABONATE AND TOBRAMYCIN
More information(ACE) inhibitor class that is primarily used in cardiovascular. conditions. Lisinopril was introduced into therapy in the early
107 CHAPTER 5 METHODDEVOLOPMENT FOR SIMULTANEOUS DETERMINATION OF LISINOPRIL AND HYDROCHLOROTHIAZIDE RELATED IMPURITIES IN LISINOPRIL AND HYDROCHLOROTHIAZIDE COMBINED TABLET DOSAGE FORMS USING HPLC 108
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ETORICOXIB AND THIOCOLCHICOSIDE IN PHARMACEUTICAL DOSAGE FORMS
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ETORICOXIB AND
More informationResearch Article. ISSN Available online at 746
ISSN 2395-3411 Available online at www.ijpacr.com 746 Research Article Development and Validation of New Analytical Methods for Simultaneous Estimation of Ramipril and Metoprolol succinate by HPLC method
More informationESTIMATION OF RELATED SUBSTANCES OF FEBUXOSTAT IN BULK & 40/80/120mg TABLETS BY RP-HPLC
Page1 Research Article International Journal of Pharmaceutical Biological and Chemical Sciences ESTIMATION OF RELATED SUBSTANCES OF FEBUXOSTAT IN BULK & 40/80/120mg TABLETS BY RP-HPLC M.NARESH CHANDRA
More informationSimultaneous For the Estimation of Metformin and Empagliflozin in Pharmaceutical Dosage Form by HPLC Method
IOSR Journal Of Pharmacy And Biological Sciences (IOSR-JPBS) e-issn:2278-3008, p-issn:2319-7676. Volume 14, Issue 1 Ser. III (Jan Feb 2019), PP 75-80 www.iosrjournals.org Simultaneous For the Estimation
More informationIAJPS 2018, 05 (01), J. Amutha Iswarya Devi et al ISSN Available online at:
CODEN [USA]: IAJPBB ISSN: 2349-7750 INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES http://doi.org/10.5281/zenodo.1174186 Available online at: http://www.iajps.com Research Article ANALYTICAL METHOD DEVELOPMENT
More informationStability Indicating Method Development and Validation for the Estimation of Rotigotine by RP-HPLC in Bulk and Pharmaceutical Dosage Form
ORIENTAL JOURNAL OF CHEMISTRY An International Open Free Access, Peer Reviewed Research Journal www.orientjchem.org ISSN: 0970-020 X CODEN: OJCHEG 2015, Vol. 31, No. (4): Pg. 2499-2505 Stability Indicating
More informationAvailable online Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(9):951-960 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Analytical method development and validation for
More informationMethod Development and Validation for Simultaneous Estimation of Atorvastatin and Ezetimibe in Pharmaceutical Dosage Form by HPLC
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationDevelopment and Validation of RP-HPLC Method for the Simultaneous Estimation of Haloperidol and Trihexyphenidyl in API and Combined Tablet Dosage Form
Amulya ORIGINAL E et al.: Development RESEARCH and Validation of RP-HPLC Method ISSN: 2455-8095 doi: https://doi.org/10.21477/ijapsr.3.3.3 Development and Validation of RP-HPLC Method for the Simultaneous
More informationResearch and Reviews: Journal of Pharmaceutical Analysis
Research and Reviews: Journal of Pharmaceutical Analysis RP-HPLC Method Development and Validation for the Determination of Bupropion Hydrochloride in a Solid Dosage Form. Lovekesh Mehta, and Jitender
More informationMEDAK DIST. ANDHRA PRADESH STATE, INDIA. Research Article RECEIVED ON ACCEPTED ON
Page67 Available Online through IJPBS Volume 1 Issue 2 APRIL- JUNE 2011 SIMPLE QUANTITATIVE METHOD DEVELOPMENT AND VALIDATION OF VALSARTAN IN PUREFORM AND PHARMACEUTICAL DOSAGE FORMS BYUV SPECTROSCOPY
More informationStress Degradation Studies And Validation Method For Quantification Of Aprepitent In Formulations By Using RP-HPLC
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.5, No.4, pp 1462-1468, April-June 2013 Stress Degradation Studies And Validation Method For Quantification Of Aprepitent
More informationValidated UV Spectrophotometric Method Development And Stability Studies Of Acamprosate Calcium In Bulk And Tablet Dosage Form
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.3, pp 1241-1246, July-Sept 2013 Validated UV Spectrophotometric Method Development And Stability Studies Of Acamprosate
More informationAvailable online at Scholars Research Library
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (1):303-314 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationDhull Rohit, Kumar Sanjay, Jalwal Pawan Department of Chemistry, OPJS Churu, Rajasthan, India
International Journal of Advanced Science and Research ISSN: 2455-4227, Impact Factor: RJIF 5.12 www.allsciencejournal.com Volume 2; Issue 2; March 2017; Page No. 31-40 Validated gradient stability indicating
More informationANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF SOLID DOSAGE FORM OF ANTINEOPLASTIC DRUG IMATINIB MESILATE BY RP HPLC.
ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF SOLID DOSAGE FORM OF ANTINEOPLASTIC DRUG IMATINIB MESILATE BY RP HPLC. G. Sathwik 1, Sonal Dubey* 1, P. Prabitha 1 and D. R. Harish Kumar 2 1 Krupanidhi
More informationMethod development and validation for the simultaneous estimation of saxagliptin and metformin in tablet dosage form by RP-HPLC method
IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: ISSN: 2320-2831 Research article Open Access Method development and validation for the simultaneous estimation of saxagliptin and metformin in tablet
More informationJournal of Global Trends in Pharmaceutical Sciences
An Elsevier Indexed Journal ISSN-2230-7346 Journal of Global Trends in Pharmaceutical Sciences METHOD DEVELOPMENT AND VALIDATION OF ANASTROZOLE IN TABLET DOSAGE FORM BY RP-HPLC METHOD Divya T 1*, Pavani
More informationRP-HPLC Method for the Simultaneous Estimation of Sitagliptin Phosphate and Metformin Hydrochloride in Combined Tablet Dosage Forms
Est. 1984 ORIENTAL JOURNAL OF CHEMISTRY An International Open Free Access, Peer Reviewed Research Journal www.orientjchem.org ISSN: 0970-020 X CODEN: OJCHEG 2012, Vol. 28, No. (1): Pg. 463-469 RP-HPLC
More informationDETERMINATION OF OXYCODONE CONTENT AND RELATED SUBSTANCES IN OXYCODONE AND ACETAMINOPHEN CAPSULE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
DETERMINATION OF OXYCODONE CONTENT AND RELATED SUBSTANCES IN OXYCODONE AND ACETAMINOPHEN CAPSULE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY *Liandong Hu 1,2 and Yanjing Shi 1,2 1 School of Pharmaceutical
More informationResearch Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form
Research Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form Gowekar NM, Lawande YS*, Jadhav DP, Hase RS and Savita N. Gowekar Department
More informationASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC
ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC METHOD APPROVALS Norvin Pharma Inc. Author Analytical Laboratory Approver Analytical Laboratory Group Leader Approver Manager Quality Control Chemistry
More informationDevelopment and Validation of RP-HPLC Method for the Estimation of Gemigliptin
Human Journals Research Article September 2018 Vol.:13, Issue:2 All rights are reserved by Hajera Khan et al. Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin Keywords: Gemigliptin,
More informationAvailable online at Scholars Research Library
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (3):157-161 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationInternational Journal of Farmacia
International Journal of Farmacia Journal Home page: www.ijfjournal.com Stability indicating analytical method development and validation for estimation of Sacubitril and Valsartan in bulk and pharmaceutical
More informationQuetiapine Tablets. Expert Committee Monographs Chemical Medicines 4 Reason for Revision Compliance
Quetiapine Tablets Type of Posting Revision Bulletin Posting Date 25 Sep 2015 Official Date 01 Nov 2015 Expert Committee Monographs Chemical Medicines 4 Reason for Revision Compliance In accordance with
More informationAsian Journal of Chemistry Vol. 21, No. 1 (2009),
Asian Journal of Chemistry Vol. 21, o. 1 (2009), 176-182 imultaneous Estimation of Related Impurities of Tizanidine Hydrochloride in its Active Pharmaceutical Ingradient by Reversed-Phase Liquid Chromatography
More informationRP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR CILOSTAZOL IN TABLET DOSAGE FORM
IJPAR Vol.5 Issue 4 Oct - Dec -2016 Journal Home page: ISSN:2320-2831 Research article Open Access RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR CILOSTAZOL IN TABLET DOSAGE FORM *1 M. Swapna *, 2 M.Vijay
More informationSanjog Ramdharane 1, Dr. Vinay Gaitonde 2
JPSBR: Volume 5, Issue 2: 2015 (151-155) ISS. 2271-3681 A ew Gradient RP- HPLC Method for Quantitative Analysis of : (3-luoro-4- Morpholin-4-yl-Phenyl)-Carbamic Acid Methyl Ester and its Related Substances
More informationA. Prameela Rani*, K.E.Pravallika, P.Ravi, O.Sasivardhan
International Journal of ChemTech Research CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555 Vol.10 No.9, pp 467-476, 2017 Development and Validation of Stability Indicating RP-UPLC Method for
More informationInt. J. Pharm. Sci. Rev. Res., 30(1), January February 2015; Article No. 32, Pages:
Research Article Development and Validation of Stability Indicating RP-HPLC Method for the Estimation of Cilnidipine in Bulk and Pharmaceutical Dosage Form. Atul Kadam* 1, Dr. (Mrs.) Purnima Hamrapurkar
More information