ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PAIN RELIEF HERBAL FORMULATIONS
|
|
- Wilfred Newton
- 6 years ago
- Views:
Transcription
1 Page5112 Indo American Journal of Pharmaceutical Research, 2016 ISSN NO: ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PAIN RELIEF HERBAL FORMULATIONS Rucha J. Shah 1, Ajay I. Patel 1, Kartik V. Vikani 2, Nisha L. Patel 1 1 B. K. Mody Govt. Pharmacy College affiliated to Gujarat Technological University, Ahmadabad, Gujarat, India. 2 Aum Research Laboratories, Rakanpur, Gandhinagar, Gujarat, India. ARTICLE INFO Article history Received 03/04/2016 Available online 30/04/2016 Keywords Menthol, Methyl Salicylate, Linseed Oil, Gas Chromatography. ABSTRACT A rapid, sensitive, precise, and Robust Gas chromatography(gc) method was developed and validated for the simultaneous estimation of Menthol, Methyl salicylate and Linseed oil in Laboratory prepared pain relief herbal formulation and marketed pain relief formulation. The determination was carried out on capillary gas chromatography using flame ionization detector. Good separation of menthol (t R = ), methyl salicylate (t R = ) and linseed oil (t R = and ) and terpene hydrate (t R = ) was obtained. The recovery of Menthol, Methyl Salicylate and Linseed oil was found to be 98.34%, 99.1% and % respectively. The correlation coefficients of linear regression analysis (r 2 ) were found to be 0.999, and respectively. Thus Proposed GC method provides a good resolution of Menthol. Methyl salicylate and Linseed oil. Corresponding author Rucha J. Shah M.Pharm. Student, Department of Quality Assurance, B. K. Mody Govt. Pharmacy College, Rajkot (Mo): rucha.j.shah@gmail.com Please cite this article in press as Rucha J. Shah et al. Analytical Method Development and Validation of Pain Relief Herbal Formulations. Indo American Journal of Pharmaceutical Research.2016:6(04). Copy right 2016 This is an Open Access article distributed under the terms of the Indo American journal of Pharmaceutical Research, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
2 Page5113 INTRODUCTION Plants have been used medicinally for thousands of years by cultures all over the world and are the main source of medicine throughout human history. Herbs and products containing herb(s) have been in trade and commerce and are currently used for a variety of purposes. As a country, India has a rich history of use of herbs, processed herbs and formulations containing herbs both from traditional wisdom as well as cultural usage. [1] All modern medicines are derived originally from traditional herbal sources. These have evolved to produce the conventional medicine, which uses both synthetic drugs and isolated natural compounds. Herbal medicines are popular among the public and improvements in their formulation resulted in a new generation of phytomedicines that are more potent than before. Since time immemorial, various herbs and their derived compounds have been used in treatment of inflammation. [2] Inflammation is a normal, protective response to tissue injury caused by physical trauma, noxious chemicals or microbiologic agents, which is a part of the host defense. Sometimes, inflammation seems to produce events that are quite serious and become chronic like occurrence of rheumatoid arthritis and hay fever which may be life threatening. Rubor (redness), calor (heat), tumor (swelling) and dolor (pain) are the main signs of inflammation. [3] Menthol, Methyl salicylate and Linseed oil are presents in many pain relief, anti-inflammatory ointments. There is no method available for simultaneous estimation of Menthol, Methyl salicylate and Linseed oil. Menthol and Methyl salicylate are volatile in nature while Linseed oil is fatty acid. So, it is required to develop a method in which thre is no heat. In this research work we have developed a simple, optimized and validated GC method for the simultaneous estimation of Menthol, Methyl salicylate and Linseed oil. The method was validated on the basis of its linearity, precision, accuracy, Limit of detection and limit of quantification. EXPERIMENTAL Chromatographic conditions: Gas Chromatography was performed on Shimadzu GC2010 apparatus, equipped with split-splitless injector attached to DB- 01 column (30 m 0.53 mm, film thickness 3 µm) and attached to flame-ionization detector (FID).Operating conditions were set as follow: carrier gas was N 2. Temperature were set as f C/min. Data equisition and integration was performed using GCsolution software. MATERIALS Standard of menthol, methyl salicylate, linseed oil and terpene hydrate were given by Aum research laboratories and formulation was prepared in lab. Preparation of standard solution: Weigh accurately 200 mg of menthol, 300 mg of methyl salicylate, 200 mg of linseed oil and 200 mg of terpene hydrate as internal standard. Transfer it in 100 ml volumetric flask. Add 50 ml of 0.05 M methanolic KOH (700 mg KOH in 250 ml methanol). Keep this solution on magnetic stirrer for 1 hr. After 1 hr add 280 mg of tartaric acid for precipitation of KOH. Shake it well. Centrifuge it for 3 min. and then filter it with whatmann filter paper. Take this filter for GC injection. Preparation of sample solution Weigh accurately 10 gm of laboratory prepare ointment and 4.5 gm of marketed ointment in 100 ml volumetric flask. Add 50 ml of 0.05 M methanolic KOH (700 mg KOH in 250 ml methanol). Keep this solution on magnetic stirrer for 1 hr. After 1 hr add 280 mg of tartaric acid for precipitation of KOH. Shake it well. Centrifuge it for 3 min. and then filter it with whatmann filter paper. Take this filter for GC injection. Figure 1: chromatogram of Standard mixture.
3 Page5114 Validation parameters The method was validated as per ICH guideline for system Suitability, Linearity, Accuracy, Precision, Robustness, Limit of Detection and Limit of Quantification. System suitability was performed using standard mixture (n=6) and test (n=1). Linearity of the method was performed by analyzing standard solution of analytes by the method in concentration range 1500 µg/ml to 9000 µg/ml. The accuracy of the proposed method was determined by a recovery study, carried out by adding known quantity of standards in placebo with three different concentrations and analyzed under previously optimized conditions. Precision was determined by repeatability (n=6 test) and intermediate precision (chemist change) (n=6 std and test both). Robustness of the method was evaluated by changing in column flow rate. RESULTS AND DISCUSSIONS Selection and optimization of chromatographic conditions Several trials have been taken in different solvents and different column oven temperature programming. A satisfactory result and good resolution obtained in Methanolic KOH and follow C/min.GC chromatogram for laboratory formulation and marketed formulation was developed. Figure: 2 Chromatogram of laboratory formulation. Figure: 3 Chromatogram of Marketed formulation. Validation of the proposed method The proposed method has been validated for the simultaneous determination of Menthol, Methyl salicylate and Linseed oil incommercial herbal formulation using following parameters. System suitability test Table 1: System suitability parameters for Menthol, Methyl salicylate and Linseed oil. Parameters Data obtained(n=6) Menthol ± RSD Methyl salicylate ± RSD Linseed oil(1) ± RSD Linseed oil(2) ± RSD Retention time ± ± ± ± 0.02 Area ± ± ± ± 1.70 Theoritical plates ± ± ± ± 1.77 Tailing factor ± ± ± ± 1.15
4 Page5115 Linearity Linear correlation was obtained between peak area Vs concentrations of Menthol and Linseed oil in the concentration ranges of µg/ml while for Methyl salicylate µg/ml. Regression parameters are mentioned in table and the calibration curves of these three markers are shown in Fig 4, Fig. 5 and Fig. 6. Figure: 4 Calibration curve of menthol. Figure: 5 Calibration curve of methyl salicylate. Figure: 6 calibration curve of linseed oil. Table 2: Regression parameter, Linearity, Limit of Detection and Limit of Quantification of the proposed GC method. Marker Concentration Range(µg/ml) Regression equation R 2 LOD (µg/ml) LOQ (µg/ml) Menthol y = 1177.x Methyl salicylate y = 856.5x Linseed oil y = 1261.x Range Range is the interval between upper and lower concentration of analyte in sample for which it has been demonstrated that the analytical method has suitable precision, accuracy and linearity. The linearity response was observed over a range of µg/ml for menthol and linseed oil and µg/ml for methyl salicylate. Accuracy The recovery experiment was performed by the standard addition method. The summary of the result and average mean of recovery data for each level within accepted range as shown in table 3.The low value of standard deviation indicates that proposed method is accurate.
5 Page5116 Table 3: Recovery data of Menthol, Methyl salicylate and Linseed oil. Drug spiked(%) Amount found Recovery Menthol Methyl salicylate Linseed oil Menthol Methyl salicylate Linseed oil ± ± ± ± ± ± ± ± ±0.28 Precision The low %RSD values of repeatability and intermediate precision parameter reveals that proposed parameter is precise. Table 4: Precision data for Menthol, Methyl salicylate and Linseed oil. Marker Repeatability(%RSD) Chemist change(%rsd) Menthol Methyl salicylate Linseed oil LOD and LOQ LOD values for Menthol, Methyl salicylate and Linseed oil were found to be µg/ml, µg/ml and µg/ml respectively. LOQ values for Menthol, Methyl salicylate and Linseed oil were found to be µg/ml, µg/ml and µg/ml respectively. These data shows that the proposed method is sensitive for the determination of Menthol, Methyl salicylate and Linseed oil. Robustness To evaluate robustness of the method was checked by changing in column flow rate. The average value of % RSD for determination of Menthol, Methyl salicylate and Linseed oil less than 2% revealed the robustness of the method. The average value of %RSD for determination of menthol, methyl salicylate and linseed oil less than 2% revealed the robustness of the method. Table 5: Robustness of the method for Menthol, Methyl salicylate and Linseed oil. Flow rate(ml/min) Menthol ± RSD Methyl salicylate ± RSD Linseed oil± RSD ± ± ± ± ± ± 0.03 Quantification of markers present in marketed formulation The three markers were found in marketed formulation and they were quantified with respect to Menthol (5%), Methyl salicylate (10%) and Linseed oil (3%) Marker % Assay Menthol % Methyl salicylate 99.55% Linseed oil 98.5% CONCLUSION Method development for simultaneous estimation in herbal formulation is difficult due to presence of so many phytoconstituents. In the present case an attempt was made to develop method for simultaneous estimation of menthol, methyl salicylate and linseed oil using GC. The developed GC method was found to be simple, precise and reliable. The method was applied to commercial polyherbal formulation and was found to be applicable for quantification of biological markers. The method can also be used for multicomponent formulation. The proposed method can be further improved by changes in column. ACKNOWLEDGEMENT Authors would like to thank Aum Research Laboratories for providing technical facilities to complete our research work.
6 Page5117 REFERENCES 1. Indian Pharmacopoeia (IP), vol III, 7 th ed. Indian Pharmacopoeia Commission publisher; 2014 pp 3169, Agnihotri S., Wakode S., Agnihotri A., An overview on anti-inflammatory properties and chemo-profiles of plants used in traditional medicine, Indian Journal of Natural Products and Resources, 2010;1(2): Jain D., Jain Y., Jain M., Anti-inflammatory Herbal Remedy-an updated review, Global Journal of Traditional Medicinal Systems,2014;3(2): C.K.Kokate, A.P. Purohit and S.B.Gokhle, Pharmacognosy; 25 th edition; Nirali Prakashan, Pune, 2003, Page No , , 315, , , ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R1); Bhairam M., Roy A.,Bahadur S., Banafar A.,Turkane D., Standerdization of herbal medicine-an overview, Journal of Applied Pharmaceutical Research,2013;10(1): Friori J.,Naldi M.,Gotti R., HS-SPME-GC-MS for the Quantitation and chiral characterization of Camphor and Menthol in creams, Springer-Chromatographia,2010;72: Kerzek J., Czekaj J., Rzeszutko, Validation of a method for simultaneous determination of menthol and methyl salicylate in pharmaceuticals by capillary gas chromatography with cool on- Acta poloniae pharmaceutica-drug research, 2003;60: Nikolic M., Markovic T., Mojovic M., Pejin B., Savic A., Peric T., et al., Chemical composition and biological activity of G b L. E -Industrial Crops and Products, 2013;49: Zhang H., Wang Z., Liu O.,Development and validation of a GC-FID method for quantitative analysis of oleic acid and related y J P A y. 11. IOFI working Group on Methods of analysis, Guidelines for the quantitative gas chromatography of volatile flavouring substances, from the working group on Methods of Analysis of the International Organization of the Flavor Industry, J. Flavour Fragr., 2011;26:
J Pharm Sci Bioscientific Res (4): ISSN NO
Development and Validation of Analytical Methods for Simultaneous Estimation of Pregabalin and Amitriptyline Hydrochloride in their Combined Marketed Dosage form ABSTRACT: Nikhilkumar Patel, Gurjit Kaur,
More informationWorld Journal of Pharmaceutical Research
World Journal of Pharmaceutical ReseaRch Volume 3, Issue 3, 4527-4535. Research Article ISSN 2277 715 DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPLC METHOD FOR ESTIMATION OF RAMOSETRON Zarana
More informationPankti M. Shah et al, Asian Journal of Pharmaceutical Technology & Innovation, 04 (17); 2016; 07-16
Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 30-03-2016 Accepted on: 01-04-2016 Published on: 15-04-2016 Corresponding Author: *Pankti M. Shah,
More informationMETHOD DEVELOPMENT AND VALIDATION BY RP-HPLC FOR ESTIMATION OF ZOLPIDEM TARTARATE
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Ramalakshmi et al. SJIF Impact Factor 6.647 Volume 7, Issue 2, 1010-1018 Research Article ISSN 2278 4357 METHOD DEVELOPMENT AND VALIDATION BY RP-HPLC
More informationISSN (Print)
Scholars Academic Journal of Pharmacy (SAJP) Sch. Acad. J. Pharm., 2014; 3(3): 240-245 Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources) www.saspublisher.com
More informationAnalysis of the fatty acids from Periploca sepium by GC-MS and GC-FID
Analysis of the fatty acids from Periploca sepium by GC-MS and GC-FID Ling Tong, Lei Zhang, Shuanghui Yu, Xiaohui Chen, Kaishun Bi * Department of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road
More informationDEVELOPMENT AND VALIDATION OF THE GC METHOD FOR THE QUANTITATIVE DETERMINATION OF SEMI-VOLATILE SOLVENTS IN THE PHARMACEUTICAL SUBSTANCE BOSENTAN
Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 71 No. 6 pp. 1107ñ1113, 2014 ISSN 0001-6837 Polish Pharmaceutical Society DEVELOPMENT AND VALIDATION OF THE GC METHOD FOR THE QUANTITATIVE DETERMINATION
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(1):138-144 Simultaneous RP HPLC determination of Latanoprost
More informationAvailable online Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2015, 7(9):951-960 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Analytical method development and validation for
More informationISSN : Method development and validation of menthol in cough syrup by gas chromatography
ISSN : 0974-7419 Volume 16 Issue 1 ACAIJ, 16(1) 2016 [001-006] Method development and validation of menthol in cough syrup by gas chromatography Ravi Kalgutkar, K.Ramakrishna, V.Srinivasarao* Department
More informationCorresponding Author:
Adv J Pharm Life sci Res, 2017 5;3:1-8 ISSN 2454 3535 (On-line) RP-HPLC Method for Estimation of Mupirocin in Bulk and Pharmaceutical Formulation S.K.Attar 1 *, M.S.Kalshetti 2, N. A. Jadhao 3, N. R. Patel
More informationSimultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by RP-HPLC method
International Journal of Chemical and Pharmaceutical Sciences 2017, Mar., Vol. 8 (1) ISSN: 0976-9390 IJCPS Simultaneous estimation of Metformin HCl and Sitagliptin in drug substance and drug products by
More informationDevelopment and Validation for Simultaneous Estimation of Sitagliptin and Metformin in Pharmaceutical Dosage Form using RP-HPLC Method
International Journal of PharmTech Research CODEN (USA): IJPRIF ISSN : 0974-4304 Vol.5, No.4, pp 1736-1744, Oct-Dec 2013 Development and Validation for Simultaneous Estimation of Sitagliptin and Metformin
More informationSimultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage Form by RP-HPLC Method
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1212-1217 Simultaneous Estimation of Gemcitabine Hydrochloride and Capecitabine Hydrochloride in Combined Tablet Dosage
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(1):6-10 ISSN: 0976-8688 CODEN (USA): PSHIBD Validated RP-HPLC method for simultaneous estimation of metformin hydrochloride
More informationSanjog Ramdharane 1, Dr. Vinay Gaitonde 2
JPSBR: Volume 5, Issue 2: 2015 (151-155) ISS. 2271-3681 A ew Gradient RP- HPLC Method for Quantitative Analysis of : (3-luoro-4- Morpholin-4-yl-Phenyl)-Carbamic Acid Methyl Ester and its Related Substances
More information36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014
36 J App Pharm Vol. 6; Issue 1: 36-42; January, 2014 Rao et al., 2014 Original Research Article SIMPLE AND RAPID LIQUID CHROMATOGRAPHIC METHOD FOR REAL-TIME QUANTIFICATION OF NAPROXEN / ESOMEPRAZOLE MAGNESIUM
More informationThe present manuscript describes simple, sensitive, rapid, accurate, precise and cost effective First derivative
ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF
More informationDevelopment and validation of stability indicating RP-LC method for estimation of calcium dobesilate in pharmaceutical formulations
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (11):236-242 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationInternational Journal of Drug Research and Technology
Int. J. Drug Res. Tech. 2016, Vol. 6 (2), 34-42 ISSN 2277-1506 International Journal of Drug Research and Technology Available online at http://www.ijdrt.com Original Research Paper DEVELOPMENT AND VALIDATION
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2015, 6(4): 30-37 ISSN: 0976-8688 CODEN (USA): PSHIBD Development and validation of stability indicating RP-HPLC method for simultaneous
More informationInternational Journal of Research in Pharmaceutical and Nano Sciences Journal homepage:
Research Article CODEX: IJRPJK ISSN: 2319 9563 International Journal of Research in Pharmaceutical and Nano Sciences Journal homepage: www.ijrpns.com HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS
More informationResearch Article Simultaneous Estimation of DL-Methionine and Pyridoxine Hydrochloride in Tablet Dosage Form by RP-HPLC
Research Article Simultaneous Estimation of DL-Methionine and Pyridoxine Hydrochloride in Tablet Dosage Form by RP-HPLC Shinde Prashanti 1 *, Mane Aruna 2, Palled Mahesh 1, Bhat AR 1 and Karagane Swapna
More informationDevelopment and Validation of Improved RP-HPLC method for Identification and Estimation of Ellagic and Gallic acid in Triphala churna
International Journal of ChemTech Research CODEN( USA): IJCRGG ISSN : 0974-4290 Vol.2, No.3, pp 1486-1493, July-Sept 2010 Development and Validation of Improved RP-HPLC method for Identification and Estimation
More informationCHAPTER INTRODUCTION OF DOSAGE FORM AND LITERATURE REVIEW
132 CHAPTER 6 DEVELOPMENT AND VALIDATION OF A STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PARACETAMOL, TRAMADOL HYDROCHLORIDE AND DOMPERIDONE IN A COMBINED DOSAGE FORM 6.1 INTRODUCTION
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS
Int. J. Chem. Sci.: 11(4), 2013, 1607-1614 ISSN 0972-768X www.sadgurupublications.com DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR QUANTITATIVE ANALYSIS TOLBUTAMIDE IN PURE AND PHARMACEUTICAL FORMULATIONS
More informationHyderabad, India. Department of Pharmaceutical Chemistry, Glocal University, Saharanpur, India.
International Journal On Engineering Technology and Sciences IJETS RP-HPLC Method development and validation for the Simultaneous Estimation of Metformin and Empagliflozine in Tablet Dosage Form Shaik
More informationComparative study of analytical method development of fluconazole in tablets and capsule by ultraviolet spectrophotometric method
Research Article Comparative study of analytical method development of fluconazole in tablets and capsule by ultraviolet spectrophotometric method Shital S. Patil*, Ubhale Ravindra, Yeole Tejal, Tayade
More informationDevelopment, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by HPLC Method
ISSN: 0973-4945; CODEN ECJAO E- Chemistry http://www.e-journals.net 2012, 9(1), 340-344 Development, Estimation and Validation of Lisinopril in Bulk and its Pharmaceutical Formulation by PLC Method V.
More informationMethod Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC
Original Research Method Development and Validation for the Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form by RP-HPLC Hanumantha Rao K 1, Lakshmana Rao A 2,*, Chandra Sekhar KB 3 1 Assistant
More informationDETERMINATION OF LOTEPREDNOL ETABONATE AND TOBRAMYCIN IN COMBINED DOSAGE FORM USING RP-HPLC METHOD
280 P a g e e-issn: 2248-9126 Vol 5 Issue 4 2015 280-284. Print ISSN: 2248-9118 Indian Journal of Pharmaceutical Science & Research www.ijpsrjournal.com DETERMINATION OF LOTEPREDNOL ETABONATE AND TOBRAMYCIN
More informationPelagia Research Library
Available online at www.pelagiaresearchlibrary.com Der Pharmacia Sinica, 2014, 5(5):91-98 ISSN: 0976-8688 CODEN (USA): PSHIBD A novel RP-HPLC method development and validation of Perindopril Erbumine in
More informationInternational Journal of Pharma and Bio Sciences
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE PHARMACEUTICAL ANALYSIS DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR ESTIMATION OF ESCITALOPRAM OXALATE IN TABLET DOSAGE
More informationAnalytical Method Development and Validation for the Estimation of Guaifenesin and Dextromethorphan by RP-HPLC
Human Journals Research Article September 2018 Vol.:13, Issue:2 All rights are reserved by Ramya Sree Borra et al. Analytical Method Development and Validation for the Estimation of Guaifenesin and Dextromethorphan
More informationAvailable online at Scholars Research Library
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (3):157-161 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationResearch Article. ISSN Available online at 746
ISSN 2395-3411 Available online at www.ijpacr.com 746 Research Article Development and Validation of New Analytical Methods for Simultaneous Estimation of Ramipril and Metoprolol succinate by HPLC method
More informationTentu Nageswara Rao et al. / Int. Res J Pharm. App Sci., 2012; 2(4): 35-40
International Research Journal of Pharmaceutical and Applied Sciences Available online at www.irjpas.com Int. Res J Pharm. App Sci., 2012; 2(4):35-40 Research Article Estimation of Fesoterodine fumarate
More informationResearch Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form
Research Article Derivative Spectrophotometric Method for Estimation of Metformin Hydrochloride in Bulk Drug and Dosage Form Gowekar NM, Lawande YS*, Jadhav DP, Hase RS and Savita N. Gowekar Department
More informationSimultaneous determination of triacetin, acetic ether, butyl acetate and amorolfine hydrochloride in amorolfine liniment by HPLC
Simultaneous determination of triacetin, acetic ether, butyl acetate and amorolfine hydrochloride in amorolfine liniment by HPLC Yuan Gao 1, Li LI 2, Jianjun Zhang 3*, Wenjuan Shu 1 and Liqiong Gao 1 1
More informationJournal of Chemical and Pharmaceutical Research, 2013, 5(1): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2013, 5(1):180-184 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 A simple and sensitive RP-HPLC method for estimation
More informationIJRPC 2011, 1(4) Rohan et al. ISSN: INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article ABSORPTION CORRECTION METHOD AND SIMULTANEOUS EQUATION METHOD FOR THE SIMULTANEOUS ESTIMATION
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com VALIDATED RP-HPLC METHOD FOR DETERMINATION OF BROMHEXINE
More informationINTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES
INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Development of standardization of trikatu churna: A spectrophotometric approach Manjesh Thakur*, P. Porwal, P. Pal, K. Shukla and S.C. Mahajan Mahakal
More informationInternational Journal of Medicine and Pharmaceutical Research. International Journal of Medicine and Pharmaceutical Research
International Journal of Medicine and Pharmaceutical Research Journal Home Page: www.pharmaresearchlibrary.com/ijmpr Research Article Open Access Development and Validation of RP-HPLC Method for the Estimation
More informationCHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG
CHAPTER 2 SIMULTANEOUS DETRMINATION OF ANASTROZOLE AND TEMOZOLOMIDE IN TEMOZOLOMIDE CAPSULES 20 MG AND ANASTROZOLE TABLETS 1 MG ANALYTICAL METHOD VALIDATION REPORT FOR ASSAY 43 2.1 Introduction Analytical
More informationIJRPC 2013, 3(1) Gandhi et al ISSN: INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT OF UV-SPECTROPHOTOMETRIC METHOD FOR THE QUANTITATIVE ESTIMATION OF OFLOXACIN AND
More informationStability indicating RP-HPLC method development and validation of Etizolam and Propranolol hydrochloride in pharmaceutical dosage form
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.org/ Original
More informationEstimation of zolmitriptan by a new RP-HPLC method
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2012, 4 (3):1022-1026 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationImpact factor: 3.958/ICV: 4.10 ISSN:
Impact factor: 3.958/ICV: 4.10 ISSN: 0976-7908 131 Pharma Science Monitor 8(3), Jul-Sep 2017 PHARMA SCIENCE MONITOR AN INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES Journal home page: http://www.pharmasm.com
More informationRapid Analysis of 37 FAMEs with the Agilent 8860 Gas Chromatograph
Application Note Food Rapid Analysis of 37 FAMEs with the Agilent 88 Gas Chromatograph Author Youjuan Zhang Agilent Technologies (Shanghai) Co. Ltd., Shanghai 131 P. R. China Abstract An Agilent 88 GC
More informationA stability indicating RP-HPLC method for simultaneous estimation of darunavir and cobicistat in bulk and tablet dosage form
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (12):89-96 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD ESTIMATION OF TOLVAPTAN IN BULK PHARMACEUTICAL FORMULATION
http://www.rasayanjournal.com Vol.4, No.1 (2011), 165-171 ISSN: 0974-1496 CODEN: RJCABP DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR AND ITS PHARMACEUTICAL FORMULATION V. Kalyana Chakravarthy * and
More informationA New Stability-Indicating and Validated RP-HPLC Method for the Estimation of Liraglutide in Bulk and Pharmaceutical Dosage Forms
OPEN ACCESS Eurasian Journal of Analytical Chemistry ISSN: 1306-3057 2017 12(2):31-44 DOI 10.12973/ejac.2017.00152a A New Stability-Indicating and Validated RP-HPLC Method for the Estimation of Liraglutide
More informationDevelopment and Validation of Area Under Curve Method for Simultaneous Estimation of Thiocolchicoside and Lornoxicam in Tablet Dosage Form
Development and Validation of Area Under Curve Method for Simultaneous Estimation of Thiocolchicoside and Lornoxicam in Tablet Dosage Form ABSTRACT: A Patel* 1, B Shah 2 1 Research Scholar, Dpt. of Pharmacy,
More informationIntroductionn Ilaprazole (ILA) is
Prajapathi et al: Simultaneous Estimation of IIaprazole and Domperi Internatio idone in Pharm maceutical Dosage Form 3549 onal Journal of Pharmaceutical Sciences and Nanotechnology Volume 9 Issue 6 November
More informationScholars Research Library. Der Pharmacia Lettre, 2015, 7 (5):44-49 (
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (5):44-49 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):770-775 Validation of Rapid Liquid Chromatographic
More informationInt. Res J Pharm. App Sci., 2012; 2(3):22-28
International Research Journal of Pharmaceutical and Applied Sciences Available online at www.irjpas.com Int. Res J Pharm. App Sci., 2012; 2(3):22-28 Research Article Validated method for the simultaneous
More informationVolume 2 (6), 2014, Page CODEN (USA)-IJPRUR, e-issn: International Journal of Pharma Research and Health Sciences
CODEN (USA)-IJPRUR, e-issn: 2348-6465 International Journal of Pharma Research and Health Sciences Available online at www.pharmahealthsciences.net Original Article Simultaneous Determination of Salbutamol
More informationDevelopment and Validation of RP-HPLC Method for the Estimation of Gemigliptin
Human Journals Research Article September 2018 Vol.:13, Issue:2 All rights are reserved by Hajera Khan et al. Development and Validation of RP-HPLC Method for the Estimation of Gemigliptin Keywords: Gemigliptin,
More informationAsian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:
Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: www.ajpamc.com ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF GEFITINIB
More informationRP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form
RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form S. LAVANYA* 1, SK. MANSURA BEGUM 1, K. NAGAMALLESWARA RAO 2, K. GAYATHRI DEVI 3 Department of pharmaceutical
More informationAbsorption Correction Method for Simultaneous Estimation of Phenazopyridine HCl and Ciprofloxacin HCl in Combined Tablet Dosage Form
JPSBR: Volume 4, Issue 2: 214 (172-176) ISSN NO. 2271-3681 Absorption Correction Method for Simultaneous Estimation of and in Combined Tablet Dosage Form ABSTRACT: Parth P. Sonara, Dr. Jignesh S. Shah
More informationSIMULTANEOUS QUANTIFICATION OF TELMISARTAN AND METOPROLOL SUCCINATE IN TABLETS BY LIQUID CHROMATOGRAPHY
World Journal of Pharmaceutical Sciences ISSN (Print): 2321-3310; ISSN (Online): 2321-3086 Published by Atom and Cell Publishers All Rights Reserved Available online at: http://www.wjpsonline.com/ Short
More informationDevelopment and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in pure and tablet dosage form
ISSN: 2320-2831 IJPAR Vol.3 Issue 4 Oct-Dec-2014 Journal Home page: Research article Open Access Development and validation of RP-HPLC method for simultaneous estimation of gliclazide and metformin in
More informationAvailable online Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2016, 8(1):171-176 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Reverse phase high performance liquid chromatography
More informationInternational Journal of Pharma and Bio Sciences DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE ANALYTICAL CHEMISTRY DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE ESTIMATION OF STRONTIUM RANELATE IN SACHET K.MYTHILI *, S.GAYATRI,
More informationMEDAK DIST. ANDHRA PRADESH STATE, INDIA. Research Article RECEIVED ON ACCEPTED ON
Page67 Available Online through IJPBS Volume 1 Issue 2 APRIL- JUNE 2011 SIMPLE QUANTITATIVE METHOD DEVELOPMENT AND VALIDATION OF VALSARTAN IN PUREFORM AND PHARMACEUTICAL DOSAGE FORMS BYUV SPECTROSCOPY
More informationfor Methotrexate. The result of analysis has been validated statistically and recovery studies confirmed the accuracy of
ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF LEFLUNOMIDE AND METHOTREXATE IN
More informationIJRPC 2013, 3(2) Jajam Thriveni et al. ISSN: INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ROSUVASTATIN CALCIUM
More informationScholars Research Library. Der Pharmacia Lettre, 2016, 8 (6): (http://scholarsresearchlibrary.com/archive.html)
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (6):217-223 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationAmudha S et al., Asian Journal of Pharmthiaceutical Technology & Innovation, 04 (21); 2016; Research Article
Asian Journal of Pharmaceutical Technology & Innovation ISSN: 2347-8810 Research Article Received on: 09-11-2016 Accepted on: 20-11-2016 Published on: 15-12-2016 Corresponding Author: * Amudha S, Dept.
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(9): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(9):70-80 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of Stability Indicating
More informationRao and Gowrisankar: Stability-indicating RP-HPLC Method for Pseudoephedrine, Ambroxol and Desloratadine
Research Paper Development and Validation of Stability-indicating RP- HPLC Method for the Estimation of Pseudoephedrine, Ambroxol and Desloratadine in Bulk and Tablet Dosage Forms N. MALLIKARJUNA RAO*
More informationAMERICAN JOURNAL OF BIOLOGICAL AND PHARMACEUTICAL RESEARCH
AMERICAN JOURNAL OF BIOLOGICAL AND PHARMACEUTICAL RESEARCH e-issn - 2348-2184 Print ISSN - 2348-2176 Journal homepage: www.mcmed.us/journal/ajbpr VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF
More informationResearch and Reviews: Journal of Pharmacy and Pharmaceutical Sciences
Research and Reviews: Journal of Pharmacy and Pharmaceutical Sciences A Validated Spectrophotometric Method for Determination of Paliperidone Palmitate in Bulk Drug and its Pharmaceutical Dosage Form.
More informationISSN: ; CODEN ECJHAO E-Journal of Chemistry 2011, 8(3),
ISSN: 0973-4945; CODEN ECJHAO E- Chemistry http://www.e-journals.net 2011, 8(3), 1275-1279 Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine
More informationDevelopment and validation of related substances method for Varenicline and its impurities
Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (1):304-309 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4
More informationAnalytical method validation for tablet of phenoxymethyl penicillin potassium by RP-HPLC method
Analytical method validation for tablet of phenoxymethyl penicillin potassium by RP-HPLC method Baghel U.S.* 1, Singhal M. 2, Gupta M.M. 1, Singh H.P. 3, Dave Shuchi 4, Sahu M. *1 Jaipur College of Pharmacy,
More informationJournal of Chemical and Pharmaceutical Research, 2018, 10(2): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2018, 10(2):84-90 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Development and Validation of Q-Absorbance Ratio
More information10 Kadam V. N. et al. Ijppr.Human, 2014; Vol. 1(2): 10-21 (Research Article) DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS FOR SIMULTANEOUS ESTIMATION OF VOGLIBOSE, GLIMEPIRIDE AND METFORMIN HYDROCHLORIDE
More informationJPSBR: Volume 4, Issue 6: 2014 ( ) ISSN NO
Analytical Method Development and Validation for Simultaneous Estimation of Timolol Maleate and Brimonidine in Bulk and Marketed Ophthalmic Formulation Suketa K. Mehta*, Dr. Dilip G. Maheshwari Department
More informationAnkit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), Available online at RESEARCH ARTICLE
Ankit et al Journal of Drug Delivery & Therapeutics; 2013, 3(2), 26-30 26 Available online at http://jddtonline.info RESEARCH ARTICLE METHOD DEVELOPMENT AND ITS VALIDATION FOR QUANTITATIVE SIMULTANEOUS
More information3-Acetyldeoxynivalenol. 15-Acetyldeoxynivalenol
3-Acetyldeoxynivalenol 15-Acetyldeoxynivalenol [Methods listed in the Feed Analysis Standards] 1 Simultaneous analysis of trichothecene mycotoxin by gas chromatography [Feed Analysis Standards, Chapter
More informationCORESTA RECOMMENDED METHOD NÄ 9
CORESTA RECOMMENDED METHOD NÄ 9 DETERMINATION OF NICOTINE IN CIGARETTE FILTERS BY GAS CHROMATOGRAPHIC ANALYSIS (April 2009) 0. INTRODUCTION In 2001 the CORESTA Routine Analytical Chemistry Sub-Group was
More informationDevelopment of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method
Research Article Development of a Validated RP-HPLC Method for the Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method * S. M. Sandhya, G. Jyothisree, G. Babu Department
More informationDEVELOPMENT AND VALIDATION OF GAS CHROMATOGRAPHY METHODS FOR THE CONTROL OF VOLATILE IMPURITIES IN THE PHARMACEUTICAL SUBSTANCE DUTASTERIDE
Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 74 No. 5 pp. 1343ñ1351, 2017 ISSN 0001-6837 Polish Pharmaceutical Society ANALYSIS DEVELOPMENT AND VALIDATION OF GAS CHROMATOGRAPHY METHODS FOR THE CONTROL
More informationJournal of Chemical, Biological and Physical Sciences
MAY 2014 JULY 2014, Vol. 4, No. 3; 2196-2204. E- ISSN: 2249 1929 Journal of Chemical, Biological and Physical Sciences An International Peer Review E-3 Journal of Sciences Available online atwww.jcbsc.org
More informationJournal of Pharmacreations
Journal of Pharmacreations ISSN: 2348-6295 Pharmacreations Vol.5 Issue 1 Jan- Mar- 2018 Journal Home page: www.pharmacreations.com Research article Open Access Method development and validation of apixaban
More informationValidation of Developed Analytical Method for Balofloxacin Floating Tablets by Reverse Phase High Performance Liquid Chromatography
International Journal of ChemTech Research CODEN (USA): IJCRGG, ISSN: 0974-4290, ISSN(Online):2455-9555 Vol.10 No.10, pp 145-155, 2017 Validation of Developed Analytical Method for Balofloxacin Floating
More informationDEVELOPMENT AND VALIDATION OF NEW HPLC METHOD FOR THE ESTIMATION OF PALIPERIDONE IN PHARMACEUTICAL DOSAGE FORMS
ISSN: 0974-1496 e-issn: 0976-0083 CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in DEVELOPMENT AND VALIDATION OF NEW HPLC METHOD FOR THE ESTIMATION OF PALIPERIDONE IN PHARMACEUTICAL
More informationA NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BERBERINE, QUERCETIN, AND PIPERINE IN AN AYURVEDIC FORMULATION
International Journal of Applied Pharmaceutics ISSN- 0975-7058 Vol 11, Issue 1, 2019 Original Article A NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF BERBERINE, QUERCETIN, AND PIPERINE IN AN AYURVEDIC
More informationDevelopment and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets
Journal of PharmaSciTech 0; ():- Research Article Development and Validation of a Simultaneous HPLC Method for Quantification of Amlodipine Besylate and Metoprolol Tartrate in Tablets * Sayyed Hussain,
More informationS. G. Talele, D. V. Derle. Department of Pharmaceutics, N.D.M.V.P. College of Pharmacy, Nashik, Maharashtra, India
ORIGINAL ARTICLE Stability-indicating high-performance liquid chromatography (HPLC) method development and validation for the determination of quetiapine fumarate in bulk and dosage form S. G. Talele,
More informationSIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD
170 Original Article SIMULTANEOUS ESTIMATION OF VALSARTAN AND HYDROCHLOROTHIAZIDE IN TABLETS BY RP-HPLC METHOD *Lakshmana Rao A, 1 Bhaskara Raju V *V.V. Institute of Pharmaceutical Sciences, Gudlavalleru,
More informationISSN (Print)
Scholars Academic Journal of Pharmacy (SAJP) Sch. Acad. J. Pharm., 2015; 4(9): 398-404 Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources) www.saspublisher.com
More informationDEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF ROSUVASTATIN CALCIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM
Research Article DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR DETERMINATION OF ROSUVASTATIN CALCIUM IN BULK AND PHARMACEUTICAL DOSAGE FORM Chirag B. Pandya*, K.P. Channabasavaraj, Jaydeep D. Chudasama,
More informationJournal of Chemical and Pharmaceutical Research
Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research J. Chem. Pharm. Res., 2010, 2(4):878-886 ISSN No: 0975-7384 CODEN(USA): JCPRC5 _ RP-HPLC method development and validation
More informationVALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DEXAMETHASONE AND GRANISETRON IN COMBINED DOSAGE FORMS
INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY Available online at www.ijrpc.com Research Article VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DEXAMETHASONE AND GRANISETRON IN COMBINED
More informationRP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms
Asian Journal of Chemistry Vol. 22, No. 7 (2010), 5067-5071 RP-HPLC Analysis of Temozolomide in Pharmaceutical Dosage Forms A. LAKSHMANA RAO*, G. TARAKA RAMESH and J.V.L.N.S. RAO Department of Pharmaceutical
More information