Lanostane Triterpenes from the Tibetan Medicinal Mushroom Ganoderma leucocontextum and Their Inhibitory Effects on HMG-CoA Reductase and α Glucosidase

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1 This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. pubs.acs.org/jnp Lanostane Triterpenes from the Tibetan Medicinal Mushroom Ganoderma leucocontextum and Their Inhibitory Effects on HMG-CoA Reductase and α Glucosidase Kai Wang,,, Li Bao,, Weiping Xiong,, Ke Ma, Junjie Han, Wenzhao Wang, Wenbing Yin, and Hongwei Liu*, Downloaded via on June 30, 2018 at 17:23:29 (UTC). See for options on how to legitimately share published articles. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichenxi Road, Chaoyang District, Beijing , People s Republic of China University of Chinese Academy of Sciences, Beijing , People s Republic of China Institute of Vegetable Research, Tibet Academy of Agriculture and Animal Science, No. 157 Jinzhuxi Road, Lasa , People s Republic of China S Supporting Information * ABSTRACT: Sixteen new lanostane triterpenes, ganoleucoins A P (1 16), together with 10 known tripterpenes (17 26), were isolated from the cultivated fruiting bodies of Ganoderma leucocontextum, a new member of the Ganoderma lucidum complex. The structures of the new compounds were elucidated by extensive spectroscopic analysis and chemical transformation. The inhibitory effects of 1 26 on HMG-CoA reductase and α-glucosidase were tested in vitro. Compounds 1, 3, 6, 10 14, 17, 18, 23, 25, and 26 showed much stronger inhibitory activity against HMG-CoA reductase than the positive control atorvastatin. Compounds 13, 14, and 16 presented potent inhibitory activity against α-glucosidase from yeast with IC50 values of 13.6, 2.5, and 5.9 μm, respectively. In addition, the cytotoxicity of 1 26 was evaluated against the K562 and PC-3 cell lines by the MTT assay. Compounds 1, 2, 6, 7, 10, 12, 16, 18, and 25 exhibited cytotoxicity against K562 cells with IC50 values in the range μm. Paclitaxel was used as the positive control with an IC50 value of 0.9 μm. This is the first report of secondary metabolites from this medicinal mushroom. species of Ganoderma in Tibet in G. leucocontextum is called Zanglingzhi in the Tibet Autonomous Region and believed to possess beneficial effects on human health.12 There have been no reports of chemical investigation of G. leucocontextum due to its scarcity in nature. We cultivated this new Ganoderma species in house for the first time and conducted chemical research on the fruiting bodies obtained. As a result, 16 new lanostane triterpenes, ganoleucoins A P (1 16), together with 10 known tripterpenes (17 26) were isolated. The structures of the new compounds were elucidated by extensive spectroscopic analysis and chemical derivation (Figure 1). The inhibitory activity of compounds 1 26 against HMG-CoA reductase and α-glucosidase and their cytotoxicity against K562 and PC-3 cell lines were evaluated in vitro. Ganoderma species (Ganodermataceae, Polyporales) have a long history of being used as folk medicine for the treatment and prevention of various diseases in China and Japan.1 They contain lanostane triterpenes, meroterpenes, alkaloids, and polysaccharides as bioactive constituents. The lanostane triterpenes from Ganoderma species presented various bioactivities, including cytotoxicity,2 anti-inflammatory activity,3 hepatoprotective effect,4 and inhibitory activities against aldose reductase,5 5αreductase,6 HMG-CoA reductase,7 and α-glucosidase.8 The Tibet plateau is usually called the Roof of the World with an average altitude of 4500 m. The unique ecosystems of the Tibet plateau, such as high-cold bush and meadows, and forests, produce a great biodiversity of edible and medicinal mushrooms.9 People living in Tibet have a long history of using wild mushrooms as food and folk medicine. Among them, Ophiocordyceps sinensis is the most famous medicinal mushroom due to its wide range of pharmacological effects.10,11 Ganoderma leucocontextum was discovered as a new 2015 American Chemical Society and American Society of Pharmacognosy Received: April 14, 2015 Published: August 19,

2 Figure 1. Structures of compounds RESULTS AND DISCUSSION The ethanol extract of G. leucocontexum was partitioned between water and ethyl acetate. The ethyl acetate-soluble fraction was subjected to chromatographic separation using silica gel, ODS, Sephadex LH-20, and preparative HPLC to yield 26 triterpenoids. Ten known compounds (17 26) were identified as ganoderiol J (17), 13 ganoderic acid DM (18), 14 ganoderone A (19), hydroxy-3,7-dioxo-5-lanosta-8,24(E)-dien-26-oic acid (20), 16 3,7-dioxo-8,24(Z)-tirucalladien-26-oic acid (21), 17 ganoderiol F (22), 18 ganoderic acid SZ (23), 19 ganoderiol B (24), 20 ganoderic acid Y (25), 21 and ganodermanontriol (26) 22 by comparison of their spectroscopic data with the literature data. Ganoleucoin A (1) was isolated as a colorless powder. The molecular formula of 1 was determined to be C 30 H 44 O 6 on the basis of HRTOFMS data. The 1 H NMR spectrum of 1 showed resonances for six methyl signals [δ H 0.87 (s), 0.89 (d, J = 6.6 Hz), 1.10 (s), 1.18 (s), 1.34 (s), and 1.85 (s)], one oxygenated methylene [δ H 4.10 (d, J = 11.3 Hz) and 3.39 (d, J = 11.3 Hz)], one oxygenated methine [δ H 4.38 (dd, J = 9.2, 5.6 Hz)], and one olefinic proton [δ H 6.86 (t, J = 7.3 Hz)]. The 13 C NMR spectrum of 1 exhibited 30 carbon resonances corresponding to six methyls, nine methylenes (one oxygenated at δ C 66.0), four methines (one oxygenated carbon at δ C 73.1), four quaternary carbons, two pairs of olefinic carbons (δ C 127.1, 138.0, 145.1, and 163.6), and three carbonyl carbons (δ C 172.5, 198.6, and 222.8, respectively). A lanostane tripterpenoid skeleton was deduced for 1 based on the above NMR data (Table 1 and Table 4). The 1 H 1 H COSY correlations of H 2-1 H 2-2, H-5 H 2-6 H-7 and H 2-15 H 2-16 H-17 H-20/(H 3-21) H 2-22 H 2-23 H-24, together with the key HMBC correlations from H 3-18 to C-12, C-13, C-14, and C-17, from H 3-19 to C-1, C-5, C-9, and C-10, from H 2-28 to C-3, C-4, C-5, and C-29, from H 3-29 to C-3, C-4, C-5, and C-28, from H-5 to C-3, C-7, C-28, and C-29, from H-7 to C-5, C-6, C-8, and C-9, from H 2-12 to C-9, C-11, C-13, C-14, C-17, and C-18, from H 3-21 to C-17, C-20, and C-22, from H-24 to C-22, C-25, C-26, and C-27, and from H 3-27 to C-24, C-25, 1978 and C-26, confirmed the planar structure of 1 (Figure 2). The relative configuration of 1 was determined by ROESY experiments. The ROESY correlations of H-5 with H-7 and H 2-28, H-17 with H 3-21, H 3-18 with H-12β and H-20, H 3-19 with H-12β and H 3-29, H 3-27 with H 2 23, and H 3-30 with H-7 and H-17 assigned the α-orientation for H-5, H-7, H-17, CH 3-21, HOCH 2-28, and CH 3-30, the β-orientation for CH 3-18, CH 3-19, and CH 3-29, and the E-geometry for the C-24/C-25 double bond (Figure 3). The absolute configurations of 13R and 14R were assigned on the basis of the positive and negative Cotton effects at 245 nm (Δε = +4.2) for a π π* transition and 354 nm (Δε = 0.5) for a n π* transition by the octant rule for the α,βunsaturated ketone group. 4 Combining the relative configuration established that the absolute configuration of 1 was determined as 4R, 5R, 7S, 10S, 13R, 14R, 17R, and 20R. The molecular formula of ganoleucoin B (2) was established as C 30 H 44 O 7 by HRTOFMS data. Its 1 H and 13 C NMR spectroscopic data revealed a similar structure to 1 except for an extra oxygenated methine [δ H 4.29 (s); δ C 78.8] (Table 1 and Table 4). The location of a hydroxy group at C-12 was confirmed by the HMBC correlations from H 3-18 to C-12, C-13, C-14, and C-17 and from H-12 to C-9 and C-11 (Figure 2). The relative configurations of H-7 and H-12 were assigned as α- and β-orientation, respectively, on the basis of NOE correlations of H-12 with H 3-18 and H 3-19 and of H-7 with H-5 and H 3-30 (Figure 3). The structure of 2 was established by detailed interpretation of its 2D NMR data. As described in 1, the positive and negative Cotton effects at 237 and 317 nm indicated the 13R and 14R configurations. Ganoleucoin C (3) was assigned the molecular formula C 32 H 46 O 8 by HRTOFMS data. A detailed comparison of 1 H and 13 C NMR spectral data between 3 and 2 indicated that compound 3 was an acetylated derivative of 2 (Table 1 and Table 4). The acetyl moiety was deduced from signals due to one methyl group (δ H 2.18, s; δ C 21.2) and one carbonyl carbon (δ C 170.7). The HMBC correlations from the H-12 to the carbonyl

3 Table 1. 1 H NMR Spectral Data of Compounds 1 6 a no m b 1.59 m 1.61 m 1.49 m 1.45 m 1.58 m 2.66 m 2.64 m 2.63 m 2.54 m 2.54 m 2.67 m dt (14.6, 8.9) 2.84 dt (14.8, 9.0) 2.85 dt (14.6, 8.9) 2.77 dt (14.6, 8.9) 2.77 dt (14.8, 9.0) 2.80 dt (14.6, 8.9) 2.38 m 2.46 m 2.38 dd (15.8, 8.1) 2.47 dd (15.9, 7.9) 2.54 m 2.45 dd (16.1, 7.9) m 1.84 m 1.86 m 1.74 m 1.70 m 2.47 m m 2.52 m 2.51 m 2.19 m 2.32 m 2.71 m 1.26 m 1.25 m 1.25 m 1.65 m 1.70 m 2.05 m dd (9.2, 5.6) 4.33 dd (9.2, 5.6) 4.38 t (7.0) 4.84 t (8.4) 4.81 dd (9.0, 5.7) m 4.29 s 5.62 s 5.65 s 4.37 s 5.70 s 2.73 m m 1.80 m m 2.13 m 2.10 m 2.27 m 2.68 m 2.70 m 1.77 m 1.80 m 1.78 dd (18.8, 8.1) 2.68 dd (19.0, 8.2) 2.26 m 2.05 dd (19.0, 8.2) m 2.25 m 2.16 m 2.41 m 2.47 m 2.50 m s 0.68 s 0.88 s 0.99 s 0.81 s 0.85 s s 1.21 s 1.34 s 1.30 s 1.38 s 1.33 s m 1.54 m 1.44 m 1.60m 1.99 m 1.66 m d (6.6) 1.10 d (6.6) 0.93 d (6.5) 1.02 d (6.5) 1.16 d (6.6) 1.04 d (6.6) m 2.27 m 2.25 m 2.30 m 2.24 m 2.31 m 2.14 m 2.15 m 2.13 dt (16.1, 7.9) 2.17 m 2.19 m 2.18 dt (16.1, 8.0) m 1.16 m 1.19 m 1.17 m 1.15 m 1.22 m t (7.3) 6.88 t (7.3) 6.86 t (7.0) 6.86 t (7.1) 6.86 t (7.3) 6.88 t (7.1) s 1.84 s 1.83 s 1.85 s 1.83 s 1.85 s d (11.3) 4.05 d (11.4) 4.04 d (11.2) 4.05 d(11.3) 4.05 d (11.4) 4.03 d (11.1) 3.39 d (11.3) 3.45 d (11.4) 3.42 d (11.2) 3.48 d (11.3) 3.52 d (11.3) 3.45 d (11.1) s 1.32 s 1.32 s 1.30 s 1.29 s 1.31 s s 1.28 s 1.15 s 1.50 s 1.29 s 1.82 s s 2.22 s 2.23 s a Recorded at 500 MHz in CDCl 3, δ H in ppm, J in Hz. b m means multiplet or overlapped with other signals. carbon at δ C confirmed the acylation of the hydroxy group at C-12 (Figure 2). The NMR signal assignment was made for 3 by 2D NMR data interpretation. Similar Cotton effects observed at 250 and 347 nm between 3 and 2 confirmed the 13R and 14R configurations. Ganoleucoin D (4) had a molecular formula of C 32 H 44 O 9,as determined by HRTOFMS data. The NMR data of 4 were similar to those of 3, except for the presence of an additional ketone moiety (δ C 216.7) (Table 1 and Table 4). HMBC correlations from H-12 to C-9, C-11, C-13, C-14, C-17, and C-18, from H 3-18 to C-12, C-13, C-14, and C-17, and from H 3-30 to C-8, C-13, C-14, and C-15 indicated a carbonyl group at C-15 in 4. The structure of 4 was fully assigned by detailed elucidation of its 2D NMR spectra (Figure 2 and Figure 3). The CD spectrum of 4 showed similar Cotton effects to those of 1 3, indicative of the same 13R and 14R configurations. The molecular formula of ganoleucoin E (5) was determined as C 30 H 42 O 8 on the basis of HRTOFMS data. The 1 H and 13 C NMR spectra of 5 resembled those of 4, except for the loss of the acetyl group (Table 1 and Table 4). HMBC correlations (Figure 2) from H-5 to C-3, C-7, and C-28, from H-7 to C-5, C-6, C-8, C-9, and C-14, from H-12 to C-9, C-11, C-13, C-14, C-17, and C-18, from H 3-18 to C-12, C-13, C-14, and C-17, from H 3-19 to C-1, C-5, C-9, and C-10, from H 2-28 to C-3, C-4, C-5, and C-29, and from H 3-29 to C-3, C-4, C-5, and C-28 confirmed the substitution of the hydroxy groups at C-7 and C-12 and the carbonyl groups at C-3, C-11, and C-15 in 5, respectively. NOE correlations of H-12 with H 3-18 and H 3-19 and of H-7 with H-5 and H determined the α- and β-orientation for H-7 and H-12, respectively. Ganoleucoin F (6) was determined to have a molecular formula of C 32 H 42 O 9 on the basis of HRTOFMS data. The 1 H and 13 C NMR data of 6 were similar to those of 4, except for the presence of an additional ketone group (δ C 198.2) and the absence of one oxygenated methine (δ C 65.6) in 4 (Table 1 and Table 4). The HMBC correlations from H-5 to C-3, C-4, C-6, C-7, C-9, C-10, C-19, C-28, and C-29 and from H 2-6 to C-4, C-5, C-7, C-8, and C-10, as well as the 1 H 1 H COSY correlation of H-5 H 2-6, confirmed the location of a carbonyl group at C-7 (Figure 2). The relative configuration of H-12 was assigned as β-orientation by NOE correlations of H-12 with H 3-18 and H 3-19 (Figure 3). Accordingly, the structure of 6 was identified as described in Figure 1. Ganoleucoin G (7) possessed the molecular formula C 30 H 46 O 5 on the basis of HRTOFMS data. The NMR data of 7 were similar to those of ganoderiol F (22), except for an additional carbonyl moiety (δ C 200.6) and an oxygenated methine replacing two olefinic methines (Table 2 and Table 5). HMBC correlations (Figure 2) from H-5 to C-3, C-4, C-6, C-7, C-9, C-10, C-19, C-28, and C-29, from H-7 to C-5, C-6, C-8, C-9, and C-14, from H 2-12 to C-9, C-11, C-13, C-14, C-17, and C-18, and from H 3-30 to C-8, C-13, C-14, and C-15, together with the 1 H 1 H COSY correlations of H-5 H 2-6 H-7, determined the location of a hydroxy group at C-7 and a carbonyl group at C-11, respectively. The relative configuration of 7 was assigned by ROESY experiment (Figure 3). As described in 1, the absolute configuration at C-13 and C-14 in 7 was determined to be 13R and 14R by CD data. The molecular formula of ganoleucoin H (8) was determined to be C 30 H 44 O 5 on the basis of HRTOFMS data. A comparison 1979

4 Table 2. 1 H NMR Spectral Data of Compounds 7 12 a no m b 1.75 m 1.49 m 1.61 m 1.61 m 1.23 m 2.51 m 2.61 m 2.48 m 2.71 m 2.68 m 2.61m dt(14.8, 9.0) 2.96 dt (14.8,9.0) 2.72 dt (15.0, 8.9) 2.88 dt (14.6, 8.9) 2.91 dt (14.6, 8.9) 3.01dt (14.6, 8.9) 2.36 m 2.54 m 2.31 m 2.43 m 2.58 m 1.69 m m 2.25 m 2.19 m 1.58 m 2.28 m 2.33 m m 2.53 m 2.08 m 2.32 m 2.64 m 2.78 m 1.34 m 2.37 m 1.61 m 1.80 m 2.05 m 1.88 m dd (9.0, 5.6) 1.99 m 4.89 t (8.2) 1.37 m m 2.72 m 2.60 t (15.1) 5.65 s 4.53 s 2.79 m 2.40 m 2.64 m 2.42 m 2.84 m m 2.00 m 4.52 m m 2.15 m 2.52 m 2.70 m 2.64 m 2.63 m 1.60 m 1.64 m 1.87 m 2.30 m 2.75 dd (18.3, 8.0) 2.71 dd (18.2, 7.9) m 1.69 m 1.57 m 2.42 m 2.57 m 2.13 m s 0.82 s 0.68 s 0.98 s 0.64 s 0.86 s s 1.28 s 1.13 s 1.44 s 1.47 s 1.38 s m 1.41 m 1.39 m 1.80 m 1.73 m 2.62m d (6.6) 0.92 d (6.6) 0.94 d (6.6) 1.03 d (6.5) 1.12 d (6.5) 0.99 d (6.6) m 2.17 m 2.16 m 2.32 m 2.27 m 2.28 m 1.92 m 2.04 m 1.99 m 2.20 m 2.14 dt (15.1, 7.8) 2.14 m m 1.14 m 1.12 m 1.19 m 1.16 m 1.23 m t (6.6) 5.54 t (6.6) 5.54 t (6.6) 6.90 t (7.1) 6.84 t (7.1) 6.84 t (7.1) s 4.23 s 4.24 s s 4.33 s 4.35 s 1.86 s 1.80 s 1.82 s s 1.12 s 1.11 s 4.65 d (11.2) 4.53 d (11.7) 4.54 d (11.5) 4.08 d (11.2) 4.11 d (11.7) 4.14 d (11.5) s 1.11 s 1.10 s 1.21 s 1.20 s 1.22 s s 1.21 s 1.40 s 1.52 s 1.70 s 1.61 s m 2.69 m 2.71 m m 2.64 m 2.63 m s 1.36 s 1.38 s s a Recorded at 500 MHz in CDCl 3, δ H in ppm, J in Hz. b m means multiplet or overlapped with other signals. of NMR data between 8 and 7 indicated the loss of an oxygenated methine and the presence of an additional carbonyl moiety (δ C 201.4) in 8 (Table 2 and Table 5). Interpretation of its HSQC, HMBC, and ROESY data assigned the structure of 8 (Figure 2). NOE correlations of H β -12 with H 3-18 and H 3-19, H 3-19 with H 3-29, H-17 with H 3-21 and H 3-30, and H-5 with H 3-28 and H 3-30 determined the β-orientation for CH 3-18, CH 3-19, and CH 3-29 and the α-orientation for H-5, H-17, CH 3-28, and CH 3-30 (Figure 3), respectively. Ganoleucoin I (9) possessed a molecular formula of C 30 H 46 O 5, as determined by HRTOFMS data. The 1 H and 13 C NMR data of 9 resembled those of 7, including five singlet methyls, one doublet methyl, 10 methylenes (two oxygenated), four methines (one oxygenated), four quaternary carbons, two pairs of olefinic carbons, and two carbonyl carbons (Table 2 and Table 5). HMBC correlations from H 3-30 to C-8, C-13, C-14, and C-15 (δ C 65.9) and from H-15 (δ H 4.52, m) to C-8, C-13, C-14, C-16, and C-17, as well as the 1 H 1 H COSY correlations of H-15 H 2-16 H-17, indicated the substitution of a hydroxy group at C-15 in 9 (Figure 2). The planar structure of 9 was established by detailed analysis of its 2D NMR spectra. The relative configuration of H-15 was assigned as α-orientation by the NOE correlations of H-15 with H-17 and H 3-30 (Figure 3). The CD spectrum of 9 was similar to that of 1, indicating 13R and 14R configurations. Compound 10 was isolated as a yellow powder, and its molecular formula was determined to be C 38 H 52 O 13 by HRTOFMS data. The 1 H NMR data of 10 exhibited signals due to five tertiary methyls (δ H 0.98, 1.21, 1.39, 1.44, and 1.52), one acetyl methyl (δ H 2.23), a vinyl methyl (δ H 1.86), a secondary methyl [δ H 1.03 (d, J = 6.5 Hz)], and three oxymethines [δ H 6.90 (t, J = 7.1 Hz); δ H 4.89 (t, J = 8.2 Hz); δ H 5.65 (s)] (Table 2). The 13 C NMR and HSQC spectra showed 38 carbon resonances including seven methyls, nine methylenes (one oxygenated at δ C 66.3), six methines (two oxygenated carbons at δ C 66.2 and 79.9), four quaternary carbons, one oxygenated tetrasubstituted carbon at δ C 70.3, four olefinic carbons at δ C 141.4, 127.5, 144.9, and 156.2, and seven carbonyl carbons (δ C 170.7, 171.6, 173.0, 174.6, 192.2, 213.1, and 216.8) (Table 5). Furthermore, a lanostane triterpenoid structure identical with that of ganoleucoin D (4) was supported by HMBC and ROESY experiments (Figure 2). The remaining signals in the NMR spectra of 10 were elucidated as a 3-hydroxy-3-methylglutaryl (HMG) moiety by HMBC correlations from H-2 (δ H 2.68, 2.63) to C-1 (δ C 171.6), from H-4 (δ H 2.68, 2.63) to C-5 (δ C 174.6), and from H-6 (δ H 1.39) to C-2 (δ C 44.8), C-3 (δ C 70.3), and C-4 (δ C 44.6). The HMG moiety was attached at C-28 of the triterpenoid skeleton on the basis of HMBC correlations from H 2-28 [δ H 4.08 (d, J = 11.2 Hz), 4.65 (d, J = 11.2 Hz)] to C-1 (δ C 171.6). The absolute configuration of the chiral carbon in the HMG group was 1980

5 Table 3. 1 H NMR Spectral Data of Compounds a no m 2.27 m 2.27 m 2.12 m b 1.78 m 1.75 m 1.76 m 1.79 m dt (14.5, 9.0) 2.79 dt (14.5, 9.0) 2.77 dt (14.5, 9.0) 2.69 dt (14.6, 8.9) 2.34 m 2.35 m 2.33 m 2.46 m m 1.54 m 1.55 m 1.45 m m 2.07 m 2.06 m 2.33 m 2.23 m 2.21 m 2.20 m 2.53 m d (5.8) 5.39 d (5.8) 5.39 d (5.8) d (6.5) 5.50 d (6.5) 5.50 d (6.5) 2.11 m 1.99 m m 2.21 m 2.22 m 1.79 m 2.10 m 2.12 m 2.19 m 1.67 m m 1.95 m 1.95 m 1.99 m 1.29 m 1.26 m 1.29 m 1.21 m m 1.63 m 1.63 m 2.32 m 1.35 m 1.38 m 1.38 m 2.70 m m 1.54 m 1.54 m 2.15 m s 0.59 s 0.55 s 0.67 s s 1.20 s 1.21 s 0.94 s m 1.45 m 1.49 m 1.80 m d (6.5) 0.91 d (6.5) 0.91 d (6.5) 0.95 d (6.5) m 1.63 m 1.63 m 1.68 m 1.05 m 1.04 m 1.05 m 1.13 m m 1.62 m 1.58 m 1.60 m 1.46 m 1.46 m 1.46 m 1.45 m d (10.2) 4.99 d (9.9) 4.77 d (9.5) 3.43 d (10.2) d (11.1) 3.49 d (12.1) 3.39 d (11.5) 4.41 d (11.4) 4.19 d (11.1) 3.32 d (12.1) 3.22 d (11.5) 4.19 d (11.4) s 1.13 s 1.15 s 1.11 s s 0.86 s 0.85 s 1.08 s s 1.13.s 1.13 s 1.12 s s 1.08 s 1.07 s 1.34 s dd (16.1,8.5) 3.65 dd (16.1, 8.6) 3.50 d (7.3) 3.65 dd (16.1, 8.5) 3.59 dd (16.1,8.5) 3.60 dd (16.1, 8.6) 3.60 dd (16.1, 8.5) t (8.5) 6.01 t (8.5) 6.95 t (7.3) 5.96 t (8.5) m 2.27 m 2.44 t (7.6) 2.25 m m 2.07 m 2.16 m 2.07 m m 5.05 m 5.18 t (7.0) 5.04 m m 1.98 m 1.96 t (7.0) 1.98 m m 2.14 m 2.08 m 2.11 m m 5.05 m 5.09 t (6.3) 5.05 m s 1.68 s 1.67 s 1.66 s s 1.60 s 1.60 s 1.57 s s 1.53 s 1.60 s 1.53 s d (1.5) 6.63 d (1.5) d (1.5) 6.63 d (1.5) dd (8.2, 1.5) 6.60 dd (8.2, 1.5) 6.88 dd (8.2, 1.5) 6.60 dd (8.2, 1.5) d (8.2) 6.73 d (8.2) 6.63 d (8.2) 6.71 d (8.2) a Recorded at 500 MHz in CDCl 3, δ H in ppm, J in Hz. b m means multiplet or overlapped with other signals determined as S by using the reference method (Scheme 1) Compound 10a was obtained by amidation of 10 with (S)-1- phenylethylamine. Reduction of 10a with LiBH 4, followed by acetylation, gave 5-O-acetyl-1-[(S)-phenylethyl]mevalonamide (10b), which had the same 1 H NMR data as those of (3R)-5-Oacetyl-1-[(S)-phenylethyl]mevalonamide. 24 Compound 10 was designated as ganoleucoin J. Ganoleucoin K (11) had a molecular formula of C 36 H 48 O 12,as determined by HRTOFMS data. The 1 H and 13 C NMR data of 11 resembled those of 10, except for the absence of the acetyl Table C NMR Spectral Data of Compounds 1 6 a no a Recorded at 125 MHz in CDCl 3, δ C in ppm. group and the presence of an additional carbonyl group (δ C 197.8) instead of an oxygenated methine (δ C 66.3) in 10 (Table 2 and Table 5). The HMBC correlations from H-5 (δ H 2.28, m) and H 2-6 (δ H 2.64, m) to C-7 (δ C 197.8), as well as the 1 H 1 H COSY correlation of H-5 H 2-6, confirmed the location of a carbonyl moiety at C-7. A detailed examination of its 2D NMR spectroscopic data confirmed the structure of 11 (Figure 2). The relative configuration of H-12 was assigned as β-orientation by NOE correlations of H-12 with H 3-18 and H Ganoleucoin L (12) was obtained as a yellow powder. The molecular formula of C 36 H 48 O 11 was confirmed on the basis of HRTOFMS data. The 1 H and 13 C NMR data of 12 were similar to those of 11, except for the absence of an oxygenated methine (δ C 77.8) in 11 and the presence of an extra methylene (δ C 44.1) (Table 2 and Table 5). The HMBC correlations from H 3-18 (0.86, s) to C-12, C-13, C-14, and C-17 and from H 2-12 to C-9, C-11, C-13, C-14, C-17, and C-18 confirmed the structural variation at C-12 between 11 and 12. Structural determination and full signal assignment of 12 were solved on the basis of 2D NMR spectral analysis (Figure 2). The absolute configuration of the HMG group in compounds 11 and 12 was determined as S by using the same method as described for 10. The HMG group has been found to be conjugated in flavonoids from Oxytropis falcata, 23 triterpenoids from Piptoporus betulinus 24 and Ganoderma resinaceum, 27 and phenylpropanoids from Edgeworthia chrysantha

6 Figure 2. Key 1 H 1 H COSY and HMBC correlations of Ganoleuconin M (13) was assigned the molecular formula C 51 H 74 O 7 on the basis of HRTOFMS data. The 1 H NMR and 13 C NMR spectra of 13 exhibited signals due to nine singlet methyl groups, two doublet methyl groups, 14 methylenes 1982

7 Figure 3. Key ROESY correlations of 1, 2, 4, 6, 7, 9, 13, and 16. including one oxygenated methylene [δ H /δ C 4.42 (d, J = 11.1), 4.19 (d, J = 11.1 Hz)/69.2], four methines including one oxymethine (δ C 77.2), four quaternary carbons, one oxygenated tetrasubstituted carbon (δ C 74.1), five pairs of double bonds including five protonated olefinic carbons (δ H /δ C 5.96 (t, J =8.5Hz)/ 140.3, 5.50 (d, J = 6.5)/117.3, 5.39 (d, J = 5.8 Hz)/120.1, 5.04 (m)/124.3, and 5.05 (m)/122.5), a 1,2,4-trisubstituted aromatic ring [δ H 6.61 (dd, J = 8.2, 1.5 Hz), 6.64 (d, J = 1.5 Hz), 6.73 (d, J = 8.2 Hz); δ C 115.1, 117.1, 117.4, 124.3, 149.0, and 149.4], and two carbonyl carbons (δ C and 217.2) (Table 3 and Table 6). A detailed analysis of its 2D NMR spectra revealed the presence of ganodermanontriol or its side-chain triol diastereomers and a 12-deoxylganomycin A unit in 13. The HMBC correlations of H 2-26 (δ H 4.42 and 4.19) with C-15 (δ C 170.4) indicated that the ganodermanontriol moiety was linked with a 12-deoxylganomycin A unit by an ester linkage (C-26 to C-15 ). In its ROESY spectrum, the NOE correlations of H-2 with H-4, H-5 with H 3-14, and H-6 with H 2-8 assigned the Z and E configurations for the C-2 C-3 and C-6 C-7 double bonds, respectively. Ganodermanontriol (26) and its three stereoisomeric triols have been synthesized in an early report and have been reported to have different chemical shifts of C-24, C-25, and C-26 in the side chain. 29 To confirm the absolute configuration in the side chain of the tripterpenoid moiety in 13, compound 13 was hydrolyzed in 2% sodium methoxide methanol to afford ganodermanontriol (26). Thus, the absolute configuration of C-24 and C-25 in 13 was assigned as 24S and 25R. The molecular formula of ganoleuconin N (14) was determined as C 51 H 74 O 7 by HRTOFMS data. The NMR data of 14 were similar to those of 13, including the NMR signals due to a ganodermanontriol moeity and a farnesyl hydroquinone unit (Table 3 and Table 6). The structure of 14 was confirmed and fully assigned by detailed analysis of its 2D NMR data. In the HMBC spectrum, the correlation of H-24 at δ H 4.99 (d, J = 9.9 Hz) with the carbonyl group at δ C confirmed the linkage of a 12-deoxylganomycin A unit (A) with ganodermanontriol through an ester bond between C-24 and C-15. The absolute 1983

8 Table C NMR Spectral Data of Compounds 7 12 a no a Recorded at 125 MHz in CDCl 3, δ C in ppm. configuration of the triterpene moiety in 14 was determined by the same method as described in 13. Ganoleuconin O (15) was obtained as a yellow powder. It had thesamemolecularformulaofc 51 H 74 O 7, as determined by HRTOFMS data. The NMR data of 15 were similar to those of 13 (Table 3 and Table 6). A significant difference observed in the 1 Hand 13 C spectra of 13 and 15 lies in the farnesyl hydroquinone unit, especially for signals due to the olefins and 1,2,4-trisubstituted benzene. A detailed examination of its 2D NMR spectral data ( 1 H 1 H COSY, HSQC, HMBC, and ROESY) confirmedthepresenceofganodermanontrioland a farnesyl hydroquinone unit (B). The HMBC correlation of H-24 at δ H 4.77 (d, J = 9.5 Hz) with the carbonyl group at δ C concluded the attachment of the moiety B at C-24. The alkaline hydrolysis of 15 also afforded ganodermanontriol (26). The NOE correlations of H 2-1 with H 2-4, H 2-5 with H 3-14, andh-6 with H 2-8 suggested the E and E configurations for the C-2 C-3 and C-6 C-7 double bonds, respectively. Thus, the structure of 15 was assigned as shown in Figure Ganoleuconin P (16) was determined to have a molecular formula of C 51 H 74 O 8 on the basis of HRTOFMS data. The IR spectrum showed the presence of carbonyl (1719 and 1700 cm 1 ) groups. A comparison of NMR data between 13 and 16 indicated that 16 was a triterpene-farnesyl hydroquinone conjugate. The 1 H and 13 C NMR spectra of 16 exhibited signals corresponding to dihydroxy-3,7-dioxo-8-ene-lanosta-26-ol 30 and 12-deoxylganomycin A units (Table 3 and Table 6). Detailed analysis of the 2D NMR spectra of 13 established its structure. The HMBC correlations of H 2-26 (δ H 4.19 and 4.41) with C-15 (δ C 170.3) indicated that the dihydroxy-3,7-dioxo-8-ene-lanosta- 26-ol moiety was conjugated with the 12-deoxylganomycin A unit by an ester linkage (C-26 to C-15 ). In its ROESY spectrum, the NOE correlations of H-2 with H 2-4,H 2-5 with H 3-14, and H-6 with H 2-8 assigned the Z and E configurations for the C-2 C-3 and C-6 C-7 double bonds, respectively. Due to the structural similarity in the side chain between 13 and 16, the absolute configurations of C-24 and C-25 in 16 were deduced by NMR data comparison. The 13 C NMR data of C-22, C-23, C-24, C-25, and C-26 in 16 were almost the same as those of 13, indicating the same absolute configuration of 24S and 25R. To date, only three triterpene-farnesyl hydroquinone conjugates (ganosinensins A C) have been reported from the fruiting body of G. sinense. 31 The medicinal mushroom G. lucidum was reported to have hypolipidemic and antidiabetic effects. The intake of powdered mycelium of G. lucidum was able to decrease total plasma cholesterol, total liver triglyceride, and total liver cholesterol levels in animals. 32 The water extract of G. lucidum was confirmed to have beneficial effects in treating type 2 diabetes mellitus through the suppression of hepatic PEPCK gene expression Oxo-ganoderic acid Z and 15-hydroxy-ganoderic acid S from G. lucidum exhibited inhibitory activities against HMG Co-A reductase with IC 50 values of 22.3 and 21.7 μm, respectively. 7 Ganoderol B was found to possess α-glucosidase inhibition with an IC 50 of μm. 8 Glucosidase and HMG Co-A reductase inhibitors have been considered to be valuable compounds for the development of hypolipidemic and antidiabetic drugs Herein, we used in vitro HMG-CoA reductase and α-glucosidase inhibition assays to test the hypolipidemic and antidiabetic effects of compounds isolated from the fruiting bodies of G. leucocontextum. Ganoleuconins A D(1-4), F (6), H (8), and J N (10 14), ganoderiol J (17), ganoderic acid DM (18), 3,7-dioxo-8,24(Z)-tirucalladien-26-oic acid (21), ganoderic acid S (23), ganoderic acid Y (25), and ganodermanontriol (26) exhibited HMG-CoA reductase inhibition with IC 50 values in the range μm (Table 7). Compounds 1, 3, 6, 10 14, 17, 18, 23, 25, and 26 showed much stronger inhibitory activity than the positive control atorvastatin. Compound 10 (IC 50 = 26.4 μm) showed much stronger inhibitory activity than compound 4 (IC 50 = 97.5 μm), which indicated the significance of the HMG moiety linking at C-28 for the HMG-CoA reductase inhibitory activity. Ganoleuconins E (5), M (13), N (14), and P (16) and ganoderone A (19) showed strong inhibitory activity against α-glucosidase from yeast with IC 50 values of 12.7, 13.6, 2.5, 5.9, and 13.7 μm (Table 7). All other compounds isolated presented relatively weak inhibitory activity with IC 50 values greater than 50 μm. The inhibitory effects of compounds were further investigated using small intestinal mucosa from rats. Compounds 13, 14, and 16 showed much stronger inhibitory activity against α-glucosidase, sucrase, and maltase from rats (Table 7) than the positive control acarbose. Preliminary structure activity relationship analysis showed that the farnesyl

9 Scheme 1. Determination of the Absolute Configuration in the HMG Moiety of 10 a a Key: (a) (S)-1-phenylethylamine, DMF, Et 3 N, PyBOP, HOBt; (b) LiBH 4, THF; (c) Ac 2 O, pyridine. Table C NMR Spectral Data of Compounds a no no a Recorded at 125 MHz in CDCl 3, δ C in ppm. hydroquinone moiety in 13, 14, and 16 contributed greatly to their inhibitory activity against α-glucosidase. Triterpenes are considered major pharmacologically active compounds contributing to the antitumor efficacy of Ganoderma. Ganoderic acid D induced apoptosis in HeLa cell. 37 Ganoderiol F exhibited cytotoxicity against Lewis lung carcinoma (LLC), sarcoma-180, and T-47D cell lines in vitro 38 and induced senescence in hepatoma HepG2 cells. 39 Ganoderic acid T showed significant antitumor effects in vivo. 40 Ganoderic acid X inhibited topoisomerases and induced apoptosis of cancer cells. 41 To evaluate the cytotxicity of triterpenes isolated from G. leucocontexum, compounds 1 26 were tested for their antiproliferative activity against tumor cell lines K562 (human myelogenous leukemia) and PC-3 (human prostate cancer). As shown in Table 8, compounds 1, 2, 6, 7, 8, 10 14, 16 19, and 25 exhibited cytotoxicity against K562 cells with IC 50 values in the range 10 to 50 μm. For the PC-3 cell line, compounds 8, 14, 16, and 19 showed cytotoxicity with IC 50 values of 24.2, 14.4, 10.8, and 28.5 μm, respectively. In summary, 26 cytotoxic lanostane triterpenes (1 26) including 16 new compounds were obtained from the fruiting body of the medicinal mushroom G. leucocontextum.the inhibitory activity of compounds 1 26 against HMG-CoA reductase and α-glucosidase and cytotoxicity against K562 and PC-3 cell lines were evaluated. This is the first report of bioactive secondary metabolites from this medicinal mushroom, which expanded the chemistry of 1985

10 Table 7. HMG-CoA Reductase and α-glucosidase Inhibitory Activity of 1 26 HMG-CoA reductase inhibition (IC 50, μm) α-glucosidase inhibition (from baker s yeast) (IC 50, μm) α-glucosidase inhibition (IC 50, μm) small intestinal mucosa from rat sucrase inhibition (IC 50, μm) maltase inhibition (IC 50, μm) ± 3.5 >50 NT a NT NT ± 10.3 >50 NT NT NT ± 4.3 >50 NT NT NT ± 9.1 >50 NT NT NT 5 > ± 2.1 NT NT NT ± 4.4 >50 NT NT NT 7 >100 >50 NT NT NT ± 4.0 >50 NT NT NT 9 >100 >50 NT NT NT ± 10.2 >50 NT NT NT ± 2.9 >50 NT NT NT ± 2.7 >50 NT NT NT ± ± ± ± ± ± ± ± ± ± >100 >50 >50 >50 >50 16 > ± ± ± ± ± 2.7 >50 NT NT NT ± 1.5 > 50 NT NT NT 19 > ± 2.3 NT NT NT 20 >100 >50 NT NT NT ± 10.4 >50 NT NT NT 22 >100 >50 NT NT NT ± 2.3 >50 NT NT NT 24 >100 >50 NT NT NT ± 2.0 >50 NT NT NT ± 5.8 >50 NT NT NT positive control atorvastatin acarbose acarbose acarbose acarbose 32.1 ± ± ± ± ± 4.1 a NT means not tested. Table 8. Cytotoxicity of Compounds 1 26 K562 (IC 50, μm) PC-3 (IC 50, μm) K562 (IC 50, μm) PC-3 (IC 50, μm) ± 4.6 > ± ± ± 4.6 > ± 7.9 >200 3 >200 > ± ± ± ± ± 4.1 >200 5 >200 > ± ± ± 3.3 > ± ± ± ± >200 > ± ± ± ± >200 > ± ± ± ± ± ± ± 5.2 > ± ± ± 1.8 > ± ± ± ± ± ± 25.4 positive control paclitaxel doxorubicin positive control paclitaxel doxorubicin 0.9 ± ± ± ± 0.4 the Ganoderma species and provided evidence for the usage of G. leucocontexum as an herbal medicine and a promising source of new bioactive agents. The discovery of new bioactive lanostane triterpenes from G. leucocontextum also supports its phylogenetic taxonomy as a new member of the G. lucidum complex. EXPERIMENTAL SECTION General Experimental Procedures. Solvent used for extraction and chromatographic separation was analytical grade. TLC was carried out on silica gel HSGF 254, and compounds were visualized by spraying with 10% H 2 SO 4 and heating. Silica gel (Qingdao Haiyang Chemical Co., Ltd., People s Republic of China) and Sephadex LH-20 (Amersham Biosciences) were used for column chromatography. HPLC separation was performed on an Agilent 1200 HPLC system using an ODS column (C 18, mm, YMC Pak, 5 μm; detector: UV) with a flow rate of 2.0 ml/min. UV and IR spectral data were acquired using a ThermoGenesys-10S UV vis and Nicolet IS5 FT-IR spectrophotometer, respectively. Optical rotations were measured on an Anton Paar MCP 200 automatic polarimeter. NMR spectral data were obtained with a Bruker Avance-500 spectrometer (CDCl 3, δ H 7.26/δ C 77.16). The HSQC and HMBC experiments were optimized for and 8.0 Hz, 1986

11 respectively. HRTOFMS data were measured using an Agilent Accurate- Mass-Q-TOF LC/MS 6520 instrument. Fungal Material. The strain of G. leucocontexum was isolated from its fruiting bodies collected in the Linzhi region of Tibet. G. leucocontexum was cultured on slants of potato dextrose agar at 25 C for 10 days. Agar plugs were inoculated into a bag (18 35 cm) with the culture medium composed of 140 g of cotton seed hulls, 36 g of wood powders, 22 g of rice bran, 2 g of CaSO 4, and 120 ml of distilled water. Two hundred bags were inoculated and incubated at 25 C for 90 days in a greenhouse. The fruiting bodies obtained were identified by W.-P.X. (one of the coauthors) by comparing their morphological characteristics with those published for G. leucocontexum. 12 Extraction and Isolation. The air-dried and powdered fruiting bodies of G. leucocontexum (3 kg) were extracted three times with ethyl alcohol (3 10 L), and organic solvent was evaporated to dryness under vacuum to afford the crude extract (179 g). The ethanol extract (170 g) was partitioned between ethyl acetate and water. The EtOAc extract (43.5 g) was subjected to silica gel column chromatography (CC) using hexane ethyl acetate in a gradient elution (v/v, 100:0, 100:2, 100:5, 100:8, 100:10, 100:15, 100:20, 100:40, 100:50), followed by dichloromethane methanol elution (v/v, 100:1, 100:2, 100:3, 100:5, 100:10, 100:15, 100:20, 0:100) to give 23 fractions (GL-1 GL-23). Fraction 16 (1.42 g) eluted with hexane ethyl acetate (v/v, 100:50) was subjected to silica gel column chromatography with a CHCl 3 MeOH gradient solvent system (100:0 0:100) to yield 10 fractions. Compound 26 (121.1 mg) was obtained from fraction GL-16-3 by recrystallization in MeOH. Compounds 16 (7.2 mg, t R 27.2 min), 13 (5.1 mg, t R 28.1 min), 14 (3.5 mg, t R 25.1 min), and 15 (4.5 mg, t R 34.2 min) were isolated from fraction GL-16-8 (130.0 mg) by RP-HPLC using 81% methanol in water. Fraction 17 (3.45 g) eluted with dichloromethane methanol (v/v, 100:1) was further separated on Sephadex LH-20 CC eluted with 50% methanol in water to give 12 subfractions (GL-17-1 GL-17-12). Fraction GL-17-3 (123 mg) was further purified by Sephadex LH-20 CC eluted with 50% methanol in water to afford compounds 18 (24 mg), 19 (28.1 mg), and 20 (20 mg). Compounds 23 (31.2 mg, t R 32.2 min), 24 (20.7 mg, t R 36.5 min), and 25 (50.1 mg, t R 40 min) were obtained from fraction GL (201.2 mg) by RP-HPLC using 75% methanol in water. Fraction 19 (5.32 g) eluted with dichloromethane methanol (v/v, 100:2) was further separated on ODS CC using a gradient of methanol water (30 100%) to give 23 subfractions (GL-19 1 GL-19 23). Compounds 1 (23.2 mg, t R 32.2 min), 2 (15.2 mg, t R 34.5 min), and 3 (12.4 mg, t R 36.2 min) were obtained from fraction GL (150.5 mg) by RP-HPLC using 56% acetonitrile in water. Fraction GL (352.3 mg) was subjected to Sephadex LH-20 CC eluted with 70% methanol in water to afford five subfractions (GL GL ). Compounds 4 (5.2 mg, t R 32.3 min) and 5 (7.2 mg, t R 36.2 min) were purified from subfraction GL (35.2 mg) by RP-HPLC using 54% acetonitrile in water. Compounds 6 (6.1 mg, t R 32.1 min), 8 (5.4 mg, t R 35.2 min), and 21 (7.1 mg, t R 37.1 min) were isolated from GL (20.5 mg) by RP-HPLC using 56% acetonitrile in water. Subfraction GL (102.5 mg) was separated by RP-HPLC using 60% acetonitrile in water to afford compound 9 (43.1 mg, t R 39.2 min). Compounds 10 (23.1 mg, t R 39.8 min), 7 (13.2 mg, t R 35.1 min), and 22 (3.6 mg, t R 38.2 min) were purified from fraction GL (90.2 mg) by RP-HPLC using 71% methanol in water. Fraction GL (123 mg) was further separated by Sephadex LH-20 CC eluted with 50% methanol in water to afford compounds 11 (45 mg), 12 (43.1 mg), and 17 (7.1 mg). The physical properties and spectroscopic data of the new compounds are as follows. Ganoleuconin A (1): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 218 (4.10), 258 (2.00) nm; CD (c M, MeOH) λ max (Δε) 245 (+4.2), 354 ( 0.5) nm; IR (neat) ν max 3315, 2980, 2948, 2840, 2820, 1702, 1696, 1618, 1586, 1429, 1360, 1347, 1236, 856, 612 cm 1 ; for 1 H and 13 C NMR data see Tables 1 and 4; positive HRTOFMS m/z [M + H] (calcd for C 30 H 45 O 6, ). Ganoleuconin B (2): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 225 (4.04), 260 (1.98) nm; CD 1987 (c M, MeOH) λ max (Δε) 237 (+3.1), 317 ( 0.5) nm; IR (neat) ν max 3319, 2948, 2925, 2840, 2820, 1723, 1702, 1625, 1586, 1425, 1369, 1364, 1226, 866, 754, 612 cm 1 ; for 1 H and 13 C NMR data see Tables 1 and 4; positive HRTOFMS m/z [M + H] (calcd for C 30 H 45 O 7, ). Ganoleuconin C (3): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 220 (4.10), 260 (2.00) nm; CD (c M, MeOH) λ max (Δε) 250 (+3.2), 347 ( 0.7) nm; IR (neat) ν max 3320, 2979, 2952, 2925, 2835, 2820, 1725, 1702, 1622, 1588, 1441, 1368, 1374, 1236, 856, 612 cm 1 ; for 1 H and 13 C NMR data see Tables 1 and 4; positive HRTOFMS m/z [M + H] (calcd for C 32 H 47 O 8, ). Ganoleuconin D (4): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 216 (4.10), 262 (2.00) nm; CD (c M, MeOH) λ max (Δε) 260 (+3.6), 350 ( 0.4) nm; IR (neat) ν max 3320, 2961, 2952, 2925, 2850, 2840, 1714, 1702, 1622, 1588, 1441, 1368, 1374, 1236, 856, 615 cm 1 ; for 1 H and 13 C NMR data see Tables 1 and 4; positive HRTOFMS m/z [M + H] (calcd for C 32 H 45 O 9, ). Ganoleuconin E (5): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 225 (4.10), 260 (2.00) nm; CD (c M, MeOH) λ max (Δε) 260 (+4.5), 355 ( 0.3) nm; IR (neat) ν max 3325, 2979, 2954, 2910, 2832, 2812, 1725, 1710, 1703, 1622, 1588, 1441, 1368, 1254, 1372, 1236, 836, 612 cm 1 ; for 1 H and 13 C NMR data see Tables 1 and 4; positive HRTOFMS m/z [M + H] (calcd for C 30 H 43 O 8, ). Ganoleuconin F (6): colorless powder, [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 216 (4.10), 262 (2.00) nm; CD (c M, MeOH) λ max (Δε) 226 (+3.2), 267 (+2.4), 310 ( 0.9) nm; IR (neat) ν max 3315, 3210, 2961, 2952, 2918, 2849, 2828, 1723, 1713, 1700, 1619, 1588, 1425, 1375, 1365, 1230, 856, 736, 615 cm 1 ; for 1 H and 13 C NMR data see Tables 1 and 4; positive HRTOFMS m/z [M + H] (calcd for C 32 H 43 O 9, ). Ganoleuconin G (7): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 200 (3.50), 254 (2.10) nm; CD (c M, MeOH) λ max (Δε) 260 (+3.6), 355 ( 0.7) nm; IR (neat) ν max 3310, 2979, 2960, 2920, 2855, 2832, 1713, 1701, 1639, 1579, 1440, 1390, 1360, 870, 725, 620 cm 1 ; for 1 H and 13 C NMR data see Tables 2 and 5; positive HRTOFMS m/z [M + H] (calcd for C 30 H 47 O 5, ). Ganoleuconin H (8): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 200 (3.50), 254 (2.10) nm; CD (c M, MeOH) λ max (Δε) 225 (+3.2), 267 (+2.3), 305 ( 0.5) nm; IR (neat) ν max 3310, 2979, 2960, 2920, 2855, 2825, 1723, 1715, 1700, 1639, 1574, 1430, 1380, 1360, 870, 724, 618 cm 1 ; for 1 H and 13 C NMR data see Tables 2 and 5; positive HRTOFMS m/z [M + H] (calcd for C 30 H 45 O 5, ). Ganoleuconin I (9): colorless powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 200 (3.50), 254 (2.10) nm; CD (c M, MeOH) λ max (Δε) 255 (+3.9), 345 ( 0.8) nm; IR (neat) ν max 3325, 2980, 2948, 2910, 2845, 2835, 1702, 1685, 1619, 1557, 1424, 1378, 1360, 870, 724, 620 cm 1 ; for 1 H and 13 C NMR data see Tables 2 and 5; positive HRTOFMS m/z [M + H] (calcd for C 30 H 47 O 5, ). Ganoleucoin J (10): yellow powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 218 (4.29), 260 (2.12) nm; CD (c M, MeOH) λ max (Δε) 250 (+4.1), 352 ( 0.7) nm; IR (neat) ν max 3310, 2977, 2969, 2922, 2875, 2860, 1723, 1706, 1685, 1641, 1577, 1457, 1379, 1372, 875, 740, 615 cm 1 ; for 1 H and 13 C NMR data see Tables 2 and 5; positive HRTOFMS m/z [M + H] (calcd for C 38 H 53 O 13, ). Ganoleucoin K (11): yellow powder, [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 218 (4.10), 260 (2.12) nm; CD (c M, MeOH) λ max (Δε) 225 (+4.2), 280 (+2.4), 310 ( 0.8) nm; IR (neat) ν max 3308, 2975, 2960, 2912, 2885, 2854, 1715, 1701, 1683, 1625, 1450, 1380, 1372, 826, 739, 615 cm 1 ; for 1 H and 13 C NMR data see Tables 2 and 5; positive HRTOFMS m/z [M + H] (calcd for C 36 H 49 O 12, ). Ganoleucoin L (12): yellow powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 218 (4.20), 262 (2.00) nm; CD (c

12 M, MeOH) λ max (Δε) 224 (+3.9), 282 (+2.5), 305 ( 0.9) nm; IR (neat) ν max 3325, 2960, 2949, 2910, 2870, 2860, 1716, 1703, 1684, 1625, 1590, 1447, 1380, 1372, 1232, 872, 740, 615 cm 1 ; for 1 H and 13 C NMR data see Tables 2 and 5; positive HRTOFMS m/z [M + H] (calcd. for C 36 H 49 O 11, ). Ganoleuconin M (13): yellow powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 248 (4.12), 254 (3.99),377(2.10) nm; IR (neat) ν max 3380, 2930, 2860, 1725, 1704, 1642, 1440, 1403, 1348, 1301, 1239, 1167, 1018, 1000, 745, 649 cm 1 ; for 1 H and 13 C NMR data see Tables 3 and 6; positive HRTOFMS m/z [M + H] (calcd for C 51 H 75 O 7, ). Ganoleuconin N (14): yellow powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 245 (3.60), 254 (3.51), 377(1.99) nm; IR (neat) ν max 3452, 2925, 2820, 1725, 1725, 1630, 1460, 1423, 1345, 1301, 1234, 1190, 1128, 1023, 1000, 654 cm 1 ; for 1 H and 13 C NMR data see Tables 3 and 6; positive HRTOFMS m/z [M + H] (calcd for C 51 H 75 O 7, ). Ganoleuconin O (15): yellow powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 248 (3.52), 254 (3.40), 377 (2.00) nm; IR (neat) ν max 3452, 2939, 2829, 1719, 1680, 1635, 1462, 1425, 1348, 1301, 1234, 1190, 1128, 1023, 980 cm 1 ; for 1 H and 13 C NMR data see Tables 3 and 6; positive HRTOFMS m/z [M + H] (calcd for C 51 H 75 O 7, ). Ganoleuconin P (16): yellow powder; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 200 (3.50), 254 (3.40), 377 (2.00) nm; IR (neat) ν max 3452, 2925, 2849, 1719, 1700, 1635, 1460, 1423, 1345, 1301, 1234, 1190, 1128, 1023, 1000, 649 cm 1 ; for 1 H and 13 C NMR data see Table 3 and 6; positive HRTOFMS m/z [M + H] (calcd for C 51 H 75 O 8, ). Determination of the Absolute Configuration in Compounds (S)-1-Phenylethylamine (19 μl, 150 μmol), Et 3 N (32 μl, 225 μmol), PyBOP (58.5 mg, 115 μmol), and HOBt (20.0 mg, 150 μmol) were added to a solution of compound 10 (50.1 mg, 75 μmol) in 0.5 ml of DMF at 0 C. The mixture was stirred at room temperature for 5 h. The reaction was quenched with dilute aqueous HCl, and the organic materials were extracted with EtOAc to afford a yellow residue, which was purified by Sephadex LH-20 CC eluting with MeOH to give amide 10a (54.2 mg). Compound 10a was further transformed into 10b (5.4 mg) by the method described in an earlier report. 23 The 1 H NMR spectrum of 10b was consistent with that of (3R)-5-O-acetyl-1-[(S)-phenylethyl]mevalonamide rather than the 3S isomer reported in the literature. 39 The absolute configuration of the HMG moiety in compounds 11 and 12 (each 10.0 mg) was determined using the same method, and all the final products were proved to be (3R)-5-O-acetyl-1-[(S)-phenylethyl]mevalonamide. (3R)-5-O-Acetyl-1-[(S)-phenylethyl]mevalonamide (10b): colorless oil; [α] 25 D (c 0.1, MeOH); UV (MeOH) λ max (log ε) 248 (3.52), 254 (3.40) nm; IR (neat) ν max 3452, 2939, 1740, 1640, 1620 cm 1 ; 1 H NMR (CDCl 3, 500 MHz) δ (5H, m, C 6 H 5-1 ), 6.34 (1H, d, J = 7.3 Hz, NH), 5.14 (1H, quintet, J = 7.2 Hz, H-1 ), 4.23 (2H, t, J = 6.8 Hz, H-5), 2.41, 2.28 (each 1H, d, J = 14.4 Hz, H-2), 2.04 (3H, s, CH 3 COO), 1.85 (2H, m, H-4), 1.50 (3H, d, J = 6.8 Hz, H-2 ), 1.23 (3H, s, H-6); positive HRTOFMS m/z [M + H] (calcd for C 16 H 23 NO 3, ). Alkaline Hydrolysis of Compounds According to a published procedure, 42 compound 13 (3.0 mg) dissolved in 2% sodium methanolate methanol (4 ml) was stirred at room temperature for 1 h. The reaction mixture was neutralized with 10% acetic acid. After removal of the solvent from the filtrate under reduced pressure, the residue was dissolved in water (10 ml) and extracted with ethyl acetate (10 ml). The organic solvents were evaporated and purified by preparative TLC on silica gel (dichloromethane methanol, 20:1) to give compound 26 (1.8 mg). Compounds 14 and 15 were hydrolyzed by the same method to give compound 26. Inhibition Assay against α-glucosidase from Baker s Yeast. As described in our earlier work, 43 the bioassay was conducted using a 96-well plate, and the absorbance was determined at 405 nm using a Spectra Max 190 microplate reader (Molecular Devices Inc.). The control was prepared by adding a phosphate buffer instead of test compounds. The blank was prepared by adding phosphate buffer instead of the α-glucosidase. The inhibition rates (%) = [(OD control OD control blank) (OD test OD test blank)]/(od control OD control blank) 100%. Acarbose was utilized as the positive control with an IC 50 of μm. Inhibition Assay against α-glucosidase, Sucrase, and Maltase from Rat s Small Intestinal Mucosa. A slightly modified method of the rat intestinal mucosa assay developed by Kwon et al. was used. 44,45 A total of 1.0 g of rat-intestinal mucosa powder was suspended in 3 ml of 0.9% saline, and the suspension was sonicated at 4 C 12 times (each 30 s). After centrifugation (10000g) at4 C for 30 min, the resulting supernatant was used as the enzyme solution for the assay. Each compound dissolved in DMSO (10 μl) was mixed with 20 μl of substrate (maltose, sucrose, p-nitrophenyl-α-d-glucopyranoside, respectively), 10 μl of enzyme solution, and 60 μl of 0.1 M phosphate buffer (ph 6.9). After preincubation for 40 min, a commercial kit (GOD assay, Jiancheng Biological Engineering Institue, Najing, China) was used to test the production of glucose. The absorbance was read at 550 nm under a Spectra Max 190 microplate reader (Molecular Devices Inc.). The blank was prepared by adding potassium phosphate buffer instead of enzyme. The control was prepared by adding potassium phosphate buffer instead of test compounds. The inhibition rates (%) = [(OD control OD control blank) (OD test OD test blank)]/ (OD control OD control blank) 100%. Inhibition Assay against HMG-CoA Reductase. The HMG-CoA reductase prepared from pig liver microsomes was assayed by the previously described colorimetric method with a slight modification. 46,47 In brief, a mixture containing 10 μl of test compounds (final concentrations of 2.5, 5, 10, 20, 40, 80 μm), 20 μl of prepared enzyme (final concentration of about 4 U/mL), 40 μl of HMG-CoA (final concentration of 200 μm), and 10 μl of 0.1 mol/l potassium phosphate buffer (ph 7.0) (containing 3.5 mmol/l EDTA, 10 mmol/l dithiothreitol, and 0.1 g/l boving serum albumin) was incubated at 37 C for 5 min. After that, 20 μl of NADPH solution (final concentration of 100 μm) was added to the reaction solution and incubated for 15 min at 37 C. The assay was conducted in a 96-well plate, and the absorbance was determined at 340 nm using a Spectra Max 190 microplate reader (Molecular Devices Inc.). The blank was prepared by adding potassium phosphate buffer instead of HMG-CoA reductase. The control was prepared by adding potassium phosphate buffer instead of test compounds. The inhibition rates (%) = [(OD test OD test blank) (OD control blank OD control)]/ (OD control blank OD control) 100%. Cytotoxicity Assay. The cytotoxicity against K562 and PC-3 cell lines of compounds 1 26 was tested using the MTT method as previously reported. 48 Statistical Analyses. The bioactivity values were expressed as means of three independent experiments, and each was carried out in triplicate. ASSOCIATED CONTENT *S Supporting Information The Supporting Information is available free of charge on the ACS Publications website at. NMR spectra data of compounds 1 26 and 10b (PDF) AUTHOR INFORMATION Corresponding Author *Tel: liuhw@im.ac.cn (H.-W. Liu). Author Contributions K. Wang, L. Bao, and W.-P. Xiong contributed equally to this article. Notes The authors declare no competing financial interest. 1988