Gerry Hendrickx Regional Sales Manager Central Europe. Developments in Waters Column Chemistries : BEH Technology
|
|
- Branden Harmon
- 5 years ago
- Views:
Transcription
1 Gerry Hendrickx Regional Sales Manager Central Europe Developments in Waters Column Chemistries : BEH Technology
2 XTerra Columns: First Generation Hybrid Particles Initial Launch 1999 Advantages of XTerra Columns: Decreased peak tailing factors for basic analytes vs. silica columns. Improved high-ph stability vs. silica columns Comparable pore properties to conventional silica columns. milar bonded phases (C 18, C 8, Embedded Polar, Phenyl) as silica column, with comparable selectivities. milar particle sizes as lica (2.5, 3.5, 5, 10 µm). Improved chemical stability without major changes in separation conditions & without the drawbacks of many alternative packing materials. Neue, U. D.; et. al. Am. Lab., 1999, 31, 36 Cheng, Y.-F.; et. al. LCGC, 2000, 18, 1162
3 Introducing 2nd Generation Hybrid: Bridged Ethane-licon Hybrid Particles Waters Patented Technology No. 6,686,035 B2 Bridged Ethanes In lica Matrix Polyethoxysilane (BPES) Anal. Chem. 2003, 75, Et CH 2 CH 2 Et Et Et Et Et Et Et n 4 Et Et Et Et Tetraethoxysilane (TES) + Et Et Et CH 2 CH 2 Et Et Et Bis(triethoxysilyl)ethane (BTEE)
4 BEH Technology Particle Attributes Surface H H H H H CH 2 CH 2 H H H Reduced lanols Wall CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 Bridging Group CH 2 CH 2 Hydrophobic Group Hybrid Particle Attribute Surface hybrid groups reduce surface silanol concentration Internal bridging groups provide high interconnectivity Internal hybrid groups provide hydrophobicity RP-HPLC Consequence Reduced USP peak tailing factors for bases Increased chemical & mechanical stability Increased high ph stability of column
5 Particle Dissolution at High ph lica Particles BEH Technology TM H - H - nly 4 bonds need to be hydrolyzed (H) 4 has high solubility in water Easy to dissolve silica at ph >7 Up to 6 bonds need to be hydrolyzed Hydrophobic, lower reactivity than silica -- bonds form as others break Difficult to remove Bridge group!
6
7 Particle Property Comparisons Particle ze % Carbon, Unbonded Surface Area Pore Volume Pore Diameter Metal Content (Na, Fe, Al) Phase Ratio BEH Technology TM 1.7 µm ACQUITY UPLC TM BEH 2.5, 3.5, 5 µm XBridge TM 6.6 %C 185 m 2 /g 0.7 cm 3 /g 135 Å 10 ppm 92 m 2 /cm 3 XTerra 2.5, 3.5, 5, 10 µm 7.0 %C 179 m 2 /g 0.7 cm 3 /g 120 Å 20 ppm 91 m 2 /cm 3 Phase Ratio = (1-ε ) i V p A + s 1 ρ s As = Surface Area Vp = Pore Volume Interstitial Column Porosity (ε i ) = 0.4 Skeletal Density (ρ s ) = 2.02 g/cm 3
8 Accelerated Low-pH Stability Test 100 Probe: Methylparaben. %Initial Retention XBridge C18 Zorbax SB-C18 SunFire C18 Inertsil DS-3 Luna C18(2) Ace C Hours in 1% TFA in H 2 ph 1.0 at 80 C Gemini C18
9 ph 3 and ph 7 Peak Shape Comparisons USP Tailing Factors ph XBridge Family Desipramine Amitriptyline ph 7 Nortriptyline Amitriptyline 1 C8 Phenyl RP18 C18 Zorbax SB C18
10 Accelerated High ph Stability Test
11 Taking ph Stability to the Extremes Hours in 0.02N NaH ph 12.3 at 50 C to 50% Efficiency Loss XBridge C8 XBridge C18 XBridge Phenyl XBridge Shield RP18 XTerra MS C18 Symmetry C18 Challenge mobile phase: 0.02N NaH ph 12.3 at 50 C Test mobile phase: 50/50 (v/v) acetonitrile/water at 50 C Test probe: Decanophenone
12 Phenyl Column Ligand Stability at Low ph %Initial Retention Probe: Ethylparaben XBridge Phenyl Zorbax SB-Phenyl Luna Phenyl-Hexyl Varian Pursuit Diphenyl Zorbax Eclipse XDB Phenyl Restek Ultra Phenyl Hours in 1% TFA in H 2 ph 1.0 at 80 C
13 XBridge Columns Bonded Phase Step 1 %C Step 1 Coverag e Endcap Final %C 16.1 %C 3.2 µmol/m 2 Proprietary 17.5 %C 11.1 %C 3.1 µmol/m 2 Proprietary 12.8 %C 13.1 %C 3.2 µmol/m 2 Proprietary 14.6 %C Polar Group 16.6 %C 3.3 µmol/m 2 TMS 16.7 %C
14 Cycle Time ptimization: Building on BEH Technology First Introduced at Pittcon 2004 as ACQUITY UPLC BEH Columns Chemistry fferings Expanded at Pittcon 2005 First Introduced at HPLC 2005 Ease of Migration from HPLC to UPLC mplified Purification and Isolation
15 Scaling from HPLC to UPLC - Constant L/dp 5 µm 150 mm Injection = 5.0 µl Flow rate = 0.2 ml/min Rs (2,3) = 2.28 HPLC 3.5 µm 100 mm Injection = 3.3 µl Flow rate = 0.3 ml/min Rs (2,3) = µm 75 mm Injection = 2.5 µl Flow rate = 0.5 ml/min Rs (2,3) = 2.34 UPLC 1.7 µm 50 mm Injection = 1.7 µl Flow rate = 0.6 ml/min Rs (2,3) = 2.29
16 Scaling HPLC to UPLC : Stability-Indicating Assay Rs = Terbinafine XBridge 2.1 x 150 mm, 5 µm C 18 F = 300 µl/min 214 nm Height (µv) Deg 5 = 6, Speed 6X Rs = Terbinafine 9 ACQUITY UPLC TM BEH C x 50 mm, 1.7 µm F = 600 µl/ min 214 nm Height (µv) Deg. 5 = 15,656 Sensitivity 2.5X (same inj. Volume) Rs = 1X AU Minutes
17 New 2 nd Generation Hybrid Bridged Ethane-licon Hybrid Particles Bridged Ethanes in Hybrid Matrix -Strength, - Great Peak Shape - Wider ph Range C C C C C C Anal. Chem. 2003, 75,
18 Buffer 3 : Phosphate ph 12 AU Injection #2 0 5 Minutes Peak 4.4% XBridge C Time (Hours) Injection #400 Injection #650 AU AU Elution rder: Propranolol Diphenhydramine Nortriptyline Acenaphthene 0 5 Minutes Minutes 15 20
Dikma Technologies Inc.
Dikma HPLC / UHPLC Columns Selection Guide HPLC Column Selection Chart Sample MW Sample Solubility Separation Mode Dikma Recommended Column Page Fat Soluble Normal Phase Platisil NH 2 Platisil CN Platisil
More informationDikma Technologies Inc.
Dikma HPLC / UHPLC Columns Selection Guide Dikma Technologies Inc. www.dikmatech.com HPLC Column Selection Chart Sample MW Sample Solubility Separation Mode Dikma Recommended Column Fat Soluble Normal
More information2012 Waters Corporation 1
An Introduction to Waters HPLC and ACQUITY UPLC Column Technologies for small molecule analysis Esa Lehtorinne Esa_Lehtorinne@waters.com Tel: +358-9-5659 5659 6288 Fax: +358-9-5659 5659 6282 Waters Finland
More informationAgilent Technologies Prep LC Columns
Agilent Technologies Prep LC Columns Agilent Technologies Prep LC Columns Agilent Technologies has always taken seriously its responsibility to ensure your success. That s why all our instruments and supplies
More informationHow to Choose a Column
How to Choose a Column How to Choose a Column You can trust that products made today will be the same as products made 30 years from now. ~ Mike Costello, Senior Master Scheduler, Taunton, MA, U.S.A. Contents
More informationRoc On with These Dependable LC Columns
Roc LC s Conventional column built to be the cornerstone for your lab; pressure rated for any 400 bar system. Solid and reliable delivers the peak shape, reproducibility, ruggedness, and performance you
More informationACE C18-AR Use the Power!
combines the retention and separation characteristics of a C18 phase with the extra selectivity of an integral phenyl functional group. The combination provides extra resolving power for a wide variety
More informationACE C18-AR. A C18 bonded phase with unique selectivity. Guaranteed reproducibility. Exceptional bonded phase stability
ACE C8-AR A C8 bonded phase with unique selectivity Guaranteed reproducibility Exceptional bonded phase stability Hydrophobic and aromatic mixed mode interaction High efficiency µm, µm, µm and 0µm particles
More informationCharged Surface Hybrid C18 for High Resolution LC and LC/MS Peptide Separations
Charged Surface Hybrid C18 for High Resolution LC and LC/MS Peptide Separations Higher Quality Peptide Separations Using Hybrid Particle-Based Reversed-Phase Columns and CSH Technology Matthew Lauber,
More informationUltra Columns HPLC COLUMNS
Acetaminophen, Narcotic Analgesics.............519 Aldehydes, Ketones...........................500 Beclomethasone.............................523 Corticosteroids..............................529 Explosives..................................501
More informationNuevos Desarrollos en Columnas ACQUITY UPLC & HPLC
Nuevos Desarrollos en Columnas ACQUITY UPLC & HPLC Bàrbara Bagó Bilbao, 12 Julio 2012 2011 Waters Corporation 1 Agenda Column Manufacturing and Platform Definitions New HPLC & ACQUITY UPLC Column Chemistries
More informationACE. For increased polar retention and alternative selectivity. Alternative selectivity for method development
ACE C8-Amide For increased polar retention and alternative selectivity Alternative selectivity for method development Improved separations with polar, acidic, basic and phenolic compounds High efficiency
More informationA New Era in SFC Column Technology
B TM A ew Era in SFC Column Technology Combining state-of-the-art media manufacturing with the industry leading column technology, Viridis Supercritical Fluid Chromatography (SFC) columns bring a new level
More informationUltra Columns. also available. ordering note
: 3μm or 5μm particles; 100Å pore size Our broadest selection of stationary phases, including unique phases. High density bondings, for maximum retention. High-purity, Type B silica gives excellent peak
More informationInertSustain Phenylhexyl. A New Second Choice Column to Change Chromatographic Behavior
InertSustain Phenylhexyl A ew Second Choice Column to Change Chromatographic Behavior Providing Alternative Selectivity to C8 Phases InertSustain Phenylhexyl columns are bonded with phenylhexyl groups,
More informationRestek Ultra II HPLC Columns
Restek Ultra II HPLC Columns THE Choice for All U(HPLC) Systems November 2009 1 www.chromtech.net.au, sales@chromtech.net.au, Tel: 03 9762 2034, Fax: 03 9761 1169 Topics for Today Introducing Restek Ultra
More informationWaters Amide Column Technology For Food Analysis
Waters Amide Column Technology For Food Analysis Euan Ross Business Development Manager Chemistry perations Europe 2012 Waters Corporation 1 verview verview of HILIC Retention mechanisms and characteristics
More informationAnalytical and Preparative SFC Columns
Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica...
More informationSepax Technologies, Inc.
Sepax Technologies, Inc. Sepax Technologies, Inc. develops and manufactures products in the area of chemical and biological separations, biosurfaces and proteomics. Sepax product portfolio includes ) liquid
More informationHyper-fast & Super-rugged
Hyper-fast & Super-rugged UHPLC & HPLC columns FALL 2013 PRODUCT CATALOG Hyper-fast & Super-rugged UHPLC & HPLC columns he introduction of HALO columns, developed using innovative Fused-Core particle
More informationConverting a CHP Method for Insulin to Agilent Poroshell 120 Columns
Converting a CHP Method for Insulin to Agilent Poroshell Columns Application Note Biopharm Author Rongjie Fu Agilent Technologies (Shanghai) Co., Ltd. 4 Ying Lun Road Shanghai 3 China Abstract A regulatory
More informationUSP Method Transfer of Ziprasidone HCl from HPLC to UPLC
Mia Summers and Kenneth J. Fountain Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Updating USP Methods from HPLC to UPLC using sub-2 µm columns 85% decrease in analysis time, faster throughput
More informationAPPLICATIONS TN µm Core-Shell Particle. (continued on page 2) Page 1 of 8. For additional technical notes, visit
Kinetex 5 µm Core-Shell Columns Deliver Significant Improvements in Chromatographic Efficiency and Resolution versus Traditional Fully Porous 5 µm Columns Philip J. Koerner and Jeff Layne Phenomenex, Inc.,
More informationAm I getting the very best value from my UHPLC analyses?
Am I getting the very best value from my UHPLC analyses? Stephen Luke LC Columns Product Manager 1 Primary reasons for UHPLC use Very fast Very high resolution Column 2.1 x 50 mm Column 2.1 x 150 mm Run
More informationUltimate Core Performance to Maximize Your Investment
Thermo Scientific Accucore HPL olumns Phase Overview Ultimate ore Performance to Maximize Your Investment Phase Overview Founded on state-of-the-art ore Enhanced Technology and utilizing vast experience
More informationThe high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns.
The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns. Fast High peak capacity Low back pressure PROTEIN PEPTIDE UHPLC COLUMNS Put these new, high-performance
More informationReversed-Phase Columns (Other than ODS)
7 Reversed-Phase Columns (ther than DS) Types and characteristics of reversed-phase columns -- 9, 9 YMC-Pack Pro C8, C--------------------------- 96 YMC-Pack C 8 --------------------------------------
More informationStudies on Stationary Phase Selectivity for Solid-Core Particles
Studies on Stationary Phase Selectivity for Solid-Core Particles Richard A. enry, Wayne K. Way, Carmen T. Santasania, and David S. Bell Supelco, Div. of Sigma-Aldrich, Bellefonte, PA 6823 USA T4060 sigma-aldrich.com
More informationA Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS
A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS By Shun-Hsin Liang and Frances Carroll Abstract Vitamin K₁ and K₂ analysis is typically complex and time-consuming because these
More informationEurospher II the logical choice. Physical properties of Eurospher II silica gel: Silica gel: ultra pure, > % Metal content: < 10 ppm
HPLC SMB smometry Euroline Eurospher II Eurospher II the logical choice After more than years on the market, our Eurospher stationary phase has established a reputation for being a first-class packing
More informationYMC-Triart C18. Multi-layer hybrid material for HPLC ph and temperature stability. Innovative micro-reactor technology
YMC-Triart C18 Versatile hybrid silica based HPLC Column The Selectivity Company Multi-layer hybrid material for HPLC ph and temperature stability Innovative micro-reactor technology www.ymc.de 2 YMC-Triart
More informationYMC-Triart C18. Multilayer hybrid material for HPLC ph and temperature stability. Innovative micro-reactor technology
YMC-Triart C18 Versatile hybrid silica based HPLC column The Selectivity Company Multilayer hybrid material for HPLC ph and temperature stability Innovative micro-reactor technology www.ymc.de 2 YMC-Triart
More informationChromatogram Search Tool Search by compound name, synonym, CAS # or keyword Pinnacle II Columns
: 3μm or 5μm particles; 110Å pore size Silica at, for total control of quality and reproducibility. Excellent replacement for the original Hypersil material. Physical and chromatographic properties similar
More informationHigh-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry and Rapid Method Development
Robert Ricker Bio-Applications Scientist CABU April 5, 2 Rapid Resolution HPLC High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry and Rapid Method Development Slide 4 Ultra-Fast
More informationLC Columns - Exceed the limit. A premium inert range of LC columns delivering optimal peak shape. ProteCol -P PEEK lined
ProteCol LC Columns - Exceed the limit A premium inert range of LC columns delivering optimal peak shape. ProteCol -G Glass lined ProteCol -P PEEK lined B C A D E F A Column end cap B PEEK frit housing
More informationACQUITY UPLC WITH PDA DETECTION: DETERMINING THE SENSITIVITY LIMITS OF OXYBUTYNIN AND RELATED COMPOUNDS
ACQUITY UPLC WITH PDA DETECTION: DETERMINING THE SENSITIVITY LIMITS OF OXYBUTYNIN AND RELATED COMPOUNDS Tanya Jenkins Waters Corporation, Milford, MA, USA INTRODUCTION Some of the most challenging methods
More informationPhenyl-Hexyl. UHPLC Columns. lternate, complementary selectivity to C18 and C8 bonded phases
Phenyl-Hexyl UHPLC Columns lternate, complementary selectivity to A C8 and C8 bonded phases articularly recommended for P compounds containing aromatic groups xcellent bonded phase stability for E durable,
More informationPrep Columns. and Bulk Media. and Bulk Media
Prep Columns and Bulk Media Axia Columns...346-355 Process Chromatography...356-36 Bulk Media...356-36 Columns, Scout and Preparative...359-36 Sepra Bulk Sorbents... 36 Prep Columns and Bulk Media 345-36
More informationThermo Scientific Hypersil BDS Columns
Technical Guide Version 2 Thermo Scientific Hypersil BDS Columns Robust and rugged, delivering excellent reproducibility and peak symmetry Part of Thermo Fisher Scientific Thermo Scientific Hypersil BDS
More informationInertSustainBio C18. Rapid Separations of Proteins and Peptides
InertSustainBio C18 Rapid Separations of Proteins and Peptides High Recoveries of Proteins and Peptides The 200A pore size silica creates the opportunity to separate compounds having a molecular weight
More informationTerence Hetzel, Thorsten Teutenberg, Torsten C. Schmidt Institut für Energie- und Umwelttechnik e. V.
Terence Hetzel, Thorsten Teutenberg, Torsten C. Schmidt Institut für Energie- und Umwelttechnik e. V. Method development of cytotoxic drugs selectivity IAC Seminar, 06.03.2015 Content Introduction Definition
More informationFortis Method Development Options
Method Dev Fortis Method Development Options Choice of Stationary phase functionality Based on Ultra pure silica Reversed Phase (RP) and Normal Phase (NP) options Fortis Ho - Polar endcapped C8 - Increased
More informationA New HILIC/RP Mixed-Mode Column and Its Applications in Surfactant Analysis
A New HILIC/RP Mixed-Mode Column and Its Applications in Surfactant Analysis X. Liu, C. Pohl, Dionex Corporation, Sunnyvale, CA, USA ABSTRACT Although reversed-phase (RP) silica columns (e.g., C18 and
More informationSelectivity Comparison of Agilent Poroshell 120 Phases in the Separation of Butter Antioxidants
Selectivity Comparison of Agilent Poroshell 1 Phases in the Separation of Butter Antioxidants Application Note Food Testing & Agriculture Author Rongjie Fu Agilent Technologies (Shanghai) Co. Ltd. Abstract
More informationPerformance of an ultra low elution volume 96-well plate
Performance of an ultra low elution volume 96-well plate Claude R. Mallet, Ziling Lu, Jeff R. Mazzeo, Uwe D. Neue Waters Corporation PittCon 2003 March 10-14 2003 Orlando, Florida Today s Challenges Faced
More informationKromasil The way to peak performance in liquid chromatography
Kromasil The way to peak performance in liquid chromatography Kromasil product catalogue Kromasil is offered in a wide assortment of columns and in bulk amounts from parts of kilograms to metric tons.
More informationImpurity Profiling of Carbamazepine by HPLC/UV
Application Note: 52049 Impurity Profiling of Carbamazepine by HPLC/UV Terry Zhang, Guifeng Jiang, Thermo Fisher Scientific, San Jose, CA, USA Key Words Accela Hypersil GOLD Carbamazepine Drug Analysis
More informationThe Raptor HILIC-Si Column
The Raptor HILIC-Si Column With Raptor LC columns, Restek chemists became the first to combine the speed of superficially porous particles (also known as SPP or core-shell particles) with the resolution
More informationExtended Temperature Range Impact on HPLC Column Stability and Performance
Extended Temperature Range Impact on HPLC Column Stability and Performance Stephanie J. Marin Dale Felix, Jody Clark Selerity Technologies, Inc. Salt Lake City, UT www.selerity.com The Selerity Series
More informationC30 ISOMERS HAVE MET THEIR MATCH
C30 ISOMERS HAVE MET THEIR MATCH INTRODUCING THE NEW! Built on proven Fused-Core particle technology, the is designed to deliver fast separations ideal for lipids and isomers compared to your C18. FEATURES
More informationColumns The FIRST and ONLY sub-2 µm core-shell columns on the market
Better UHPLC Performance Starts NW.7 µm Core-Shell * Columns The IRST and NLY sub- µm core-shell columns on the market Phenomenex India Laxmi Cyber City, Ground loor, B Block, Survey no: Kondapur, Hyderabad
More informationVITAMIN B1 & B6 VITAMIN B1&B6 COMBI-DETERMINATION IN EDTA- OF HEPARINE WHOLE BLOOD. HPLC & UPLC Method
VITAMIN B1 & B6 VITAMIN B1&B6 COMBI-DETERMINATION IN EDTA- OF HEPARINE WHOLE BLOOD Method HPLC Vitamin B6 (PLP) - 1.8 min. Internal Standard - 5.7 min. Vitamin B1 (TDP) - 7.9 min. UPLC Vitamin B6 (PLP)
More informationHPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 1290 Infinity II LC
HPLC to UHPLC Transfer of USP Method for Amlodipine Besylate Using the Agilent 129 Infinity II LC Application Note Small Molecule Pharmaceuticals Authors Gerd Vanhoenacker, Mieke Steenbeke, Koen Sandra,
More informationAPPLICATIONS TN Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology.
Dramatically Improve Existing 5 µm and 3 µm Fully Porous Methods with Kinetex 5 µm Core-shell Technology Jeff Layne, Phenomenex, Inc. Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 951 USA With virtually
More informationProbing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection INTRODUCTION
Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection Michael Jones Waters Corporation, Wilmslow, UK APPLICATION BENEFITS The ACQUITY Arc System is
More informationHPLC Separation Tools for Membrane Protein Purification and Drug Membrane Permeability Prediction
IAM CHRMATGRAPHY IAM CHRMATGRAPHY HPLC Separation Tools for Membrane Protein Purification and Drug Membrane Permeability Prediction Immobilized Artificial Membrane (IAM) technology is an innovative approach
More informationACE Excel UHPLC Columns
Excel UHPLC Columns info@ace-hplc.com Advanced Chromatography Technologies Ltd www.ace-hplc.com Australia & New Zealand contact: infor@winlab.com.au or call + 0 Outline Introduction Key Product Points
More informationNUCLEODUR. Professional solutions for HPLC. An optimized phase for every field of application.
NUCLEODUR Professional solutions for HPLC An optimized phase for every field of application Contents / Customer service NUCLEODUR high purity silica Overview of NUCLEODUR phases Summary of available modifications
More informationIAM Chromatography. Introduction
IAM Chromatography IAM Drug Discovery Columns Introduction References: 1. Regis 1998-99 Chromatography Catalog A simple, rapid method to predict drug transport across biological barriers has been a long
More informationInsulin SEC analysis. Insulin Samples:
EE1 Insulin SEC analysis Insulin Samples: Lispro, identical in structure to Insulin Human, except that it has lysine and proline at positions 8 and 9, respectively, of chain B, whereas this sequence is
More informationAeris. Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations. Aeris PEPTIDE. Aeris WIDEPORE
Aeris Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations Aeris is a specialized line of reversed phase core-shell UHPLC columns, built exclusively for the ultra-high performance
More informationNew HPLC Column Options for UHPLC
New HPLC Column Options for UHPLC Rapid Resolution High Definition Columns and More Chor Koon Tan, PhD Business Development Manager Columns & Supplies Division Asia-Pacific Agilent Technologies Page 1
More informationACE. Intelligent Solutions for Method Development. Method Development Kits. ACE UHPLC and HPLC Columns. a a
r: ff e ts ice O ki r ti y mn e p tiv olu sam mn c c u le the col e d S an for ngle le si ab s a l i a a av ACE Method Development Kits Intelligent Solutions for Method Development Highly cost effective
More informationCore-Shell Technology for Proteins and Peptides
Core-Shell Technology for Proteins and Peptides Better BioSeparations on HPLC and UHPLC Systems www.phenomenex.com/aeris Aeris Core-Shell Technology Core-Shell Particles Precision Engineered for Protein
More informationExploiting Selectivity in HPLC and UHPLC With Rational Stationary Phase Design
Exploiting Selectivity in HPLC and UHPLC With Rational Stationary Phase Design Alan P McKeown amckeown@ace-hplc.com Advanced Chromatography Technologies www.ace-hplc.com www.mac-mod.com Outline 2 Chromatographic
More informationSUPPORTING INFORMATION:
1S SUPPORTING INFORMATION: The discovery of the once weekly glucagon like peptide 1 (GLP-1) analog semaglutide Jesper Lau*, Paw Bloch, Lauge Schäffer, Ingrid Pettersson, Jane Spetzler, Jacob Kofoed, Kjeld
More informationComparison of a UPLC Method across Multiple UHPLC Systems
Comparison of a UPLC Method across Multiple UHPLC Systems Tanya Jenkins Waters Corporation, Milford, MA, U.S. INTRODUCTION In 2004, Waters introduced the ACQUITY UPLC System. Since this launch, many liquid
More informationPreparative Chromatography Meets UHPLC
Preparative Chromatography Meets UHPLC The Power of Kinetex Core-Shell Technology with the Performance of Piston Packed Prep Columns Ultra-High Efficiencies Ultra-Long Lifetimes NO Added Pressure Two Award
More information176 YMC Chiral Columns
176 YMC Chiral Columns YMC Chiral Columns 177 Chiral Columns Content YMC Chiral NEA(R)(S)... 178-181 YMC Chiral CD BR... 182-185 Ordering Information... 186 HPLC Columns for Optical Isomer Separation Introduction
More informationHPLC Products. New 1.9μm Pinnacle DB Small Particle HPLC Columns. 2007/08 Edition. Chromatography Products. hplc columns.
HPLC Products 2007/08 Edition hplc columns hplc accessories hplc replacement parts analytical reference materials vials & syringes New 1.9μm Pinnacle DB Small Particle HPLC Columns See page 8 for details.
More informationCore-Shell / Solid core Particles: A Practical Substitute to Sub-2 Micron Particles: An overview
Core-Shell / Solid core Particles: A Practical Substitute to Sub-2 Micron Particles: An overview ABSTRACT: S. M. Ramdharane 1, K. B. Vyas 2, K. S. Nimavat 3 * 1 Pacific Academy of Higher Education & Research
More informationFor Far. Greater. UHPLC Column Performance You Must Have Kinetex Core-Shell UHPLC Columns.
For Far Greater UHPLC Column Performance... You Must Have Kinetex Core-Shell UHPLC Columns www.phenomenex.com/kinetex The UHPLC Core-Shell Advantage Kinetex Core-Shell Technology produces increased efficiencies
More informationChapter 22: Gas and Liquid Chromatography. Liquid Chromatography: Stationary Phases and Mobile Phases: Normal Phase: Reversed Phase:
Chapter 22: Gas and Liquid Chromatography Liquid Chromatography: Stationary Phases and Mobile Phases: Normal Phase: Reversed Phase: Ion Exchange Chromatography Page 1 of 5 Pinnacle DB Ideal for applications
More informationAgilent Anion-Exchange Media for Proteins - Loading vs Resolution - Effect of Flow Rate and Example Protein Separations
Agilent Anion-Exchange Media for Proteins - Loading vs Resolution - Effect of Flow Rate and Example Protein Separations Technical Overview Introduction PL-SAX is a hydrophilic strong anion-exchange chromatographic
More informationSeparation of Vitamin D and Vitamin D Metabolites on FLARE C18 MM (Mixed Mode) HPLC Column
Diamond Analytics Technical Note: T131-1 Separation of Vitamin D and Vitamin D Metabolites on FLARE C1 MM (Mixed Mode) HPLC Column Introduction In this technical note, the versatility of the diamond-based
More informationBiphenyl. Stationary Phase: Fast, Rugged Raptor Columns with Time-Tested Selectivity. Pure Chromatography. Selectivity Accelerated
Fast, Rugged Raptor Columns with Time-Tested Selectivity Selectivity Accelerated Stationary Phase: Biphenyl CH 3 Si CH 3 O Pure Chromatography www.restek.com/raptor The Raptor Biphenyl Column With Raptor
More informationThis revision also necessitates a change in the table numbering in the test for Organic Impurities.
Methylphenidate Hydrochloride Extended-Release Tablets Type of Posting Notice of Intent to Revise Posting Date 27 Jul 2018 Targeted Official Date To Be Determined, Revision Bulletin Expert Committee Chemical
More informationColumns for HPLC. Columns for HPLC
Analytical columns packed with NUCLEDUR NUCLEDUR reversed phase packings In reversed phase liquid chromatography the efficiency of the packing is strongly affected by the quality of the base silica itself.
More informationRapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit
Rapid Gradient and Elevated Temperature UHPLC of Flavonoids in Citrus Fruit Application Note General Chromatography, Food Industry Authors John W. Henderson Jr., Judy Berry, Anne Mack, William Long Agilent
More informationColumns for HPLC. Columns for HPLC
Basic principles of preparative HPLC Basically, preparative HPLC follows the same rules as analytical scale chromatography. However, there are important differences in the aims of the two techniques. In
More informationHPLC Columns. HPLC Accessories HPLC Column Test Mixes Reference Books...www.restek.com. HPLC Applications...
HPLC Columns HPLC Column Selection................................292-297 UHPLC Columns........................................298-299 Capillary LC Columns........................................300 Pinnacle
More informationAPPLICATIONS TN A Comparison of Various Kinetex C18 Phases USP: L1
APPLICATIONS A Comparison of Various Kinetex C18 Phases Zeshan Aqeel and Jeff Layne Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 91 USA Introduction When performing reversed phase LC separations, there
More information11:00 a.m. EST Telephone Number: Chair Person: Tim Spaeder
Stephen W. Coates LC Columns Marketing Specialist Consumables and Accessories Business Unit May 17, 2 High-Throughput Separations for QA/QC, LC/MS, LC/MS/MS, Combinatorial Chemistry, and Rapid Method Development
More informationFat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C18 Bonded Column
Fat-Soluble Vitamins Analysis on an Agilent ZORBAX Eclipse PAH Polymeric C8 Bonded Column Application Note Food Authors Rongjie FU Agilent Technologies (Shanghai) Co. Ltd. Ying Lun Road Waigaoqiao Free
More informationApplication Note. Separation of clindamycin phosphate and process impurities. Summary. Introduction. Category Pharmaceutical analysis Matrix
Application Note Separation of clindamycin phosphate and process impurities Category Pharmaceutical analysis Matrix Drugs Method UHPLC Keywords Antibiotics, process impurities, quality control Analytes
More information3 Normal-Phase Columns. Reversed-Phase C18 Columns (ODS) Reversed-Phase Columns (Other than ODS) Columns for HTS and LC/MS Analysis.
We are pleased to present the 11th YMC General Catalog. Since its formation in 1980, YMC Co., Ltd. has been working in the rapidly changing field of chemistry. Over this time, the rate of discovery of
More informationPharmacopoeia Methods
Allowable Adjustments to Pharmacopoeia Methods Isocratic Separations Component European Pharmacopoeia (Ph. Eur.) Composition of the mobile phase ± 30 % Relative or ± 2 % absolute, whichever is the larger;
More informationSystematic Evaluation of Solid Phase Extraction (SPE) Chemistries for the Determination of Acidic, Neutral, and Basic Drugs
Systematic Evaluation of Solid Phase Extraction (SPE) Chemistries for the Determination of Acidic, Neutral, and Basic Drugs Laura Marlatt, An Trinh, and David S. Bell Supelco, 595 North Harrison Road,
More informationEszopiclone (Lunesta ): An Analytical Profile
Eszopiclone (Lunesta ): An Analytical Profile Roxanne E. Franckowski, M.S.* and Robert A. Thompson, Ph.D. U.S. Department of Justice Drug Enforcement Administration Special Testing and Research Laboratory
More informationInertSustain Amide 技術資料
InertSustain Amide 技術資料 InertSustain Amide Physical Properties Silica Particle Size Surface Area Pore Size Pore Volume Bonded Phase End-capping Carbon Loading ph Range USP Code :ES (Evolved Surface) Silica
More informationRapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column
APPLICATION NOTE 21673 Rapid and sensitive UHPLC screening of additives in carbonated beverages with a robust organic acid column Authors Aaron Lamb and Brian King, Thermo Fisher Scientific, Runcorn, UK
More informationExtended Application Note
Extended Application Note Comparison of 4 μm vs. 2.o Columns in Analysis of Drospirenone/Ethinyl Estradiol Tablet APP A-339 www.mtc-usa.com 1-732-578-1777 INTRODUCTION The formulation of drospirenone and
More informationEnhanced method development workflow for modern LC and SFC
Enhanced method development workflow for modern LC and SFC Davy GUILLARME 18 th of September 2014 What can be done with modern LC? MONOLITHS HIGH TEMPERATURE 1 000 000 plates 12 m column 120 C.60 0.40
More informationBiomed Pap Med Fac Univ Palacky Olomouc Czech Repub
SUPPLEMENTAL MATERIAL Sistik P, Turjap M, Iordache AM, Saldanha H, Lemr K, Bednar P. Quantification of selected antidepressants and antipsychotics in clinical samples using chromatographic methods combined
More informationSUMMARY, CONCLUSION & RECOMMENDATIONS
196 Chapter-5 SUMMARY, CONCLUSION & RECOMMENDATIONS 197 CHAPTER 5 5.1 Summary, Conclusion and Recommendations Summary and Conclusion are drawn based on the work carried out by the author on development
More informationComparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation
Oriental Journal of Chemistry Vol. 24(2), 537-544 (2008) Comparison of conventional HPLC with UPLC method for determination of albuterol sulfate and it s impurities in pharmaceutical formulation P.N. DALVI,
More informationCore-Shell Technology. 11 HPLC Myths. Uncovered. & Why You Must Have Kinetex Core-Shell HPLC Columns
Core-Shell Technology 11 HPLC Myths Uncovered & Why You Must Have Kinetex Core-Shell HPLC Columns 2.6 www.phenomenex.com/kinetex HPLC myths have been around since the early 19 s! From the one about the
More informationMaximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system
Maximizing chromatographic peak capacity with the Agilent 1290 Infinity LC system A practical guide on how to use parameters to increase peak capacity Application Note Pharmaceutical and Chemical Author
More informationHICHROM. Chromatography Columns and Supplies NEW PRODUCTS. Catalogue 9. Hichrom Limited
HICHROM Chromatography Columns and Supplies NEW PRODUCTS Catalogue 9 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email: sales@hichrom.co.uk
More information