QUANTITATIVE ANALYSIS OF STEROIDS

Size: px
Start display at page:

Download "QUANTITATIVE ANALYSIS OF STEROIDS"

Transcription

1 - 041, STUDIES IN ANALYTICAL CHEMISTRY 5 QUANTITATIVE ANALYSIS OF STEROIDS S. G5ROG Head of Department, Chemical Works G. Richter Ltd., Honorary Professor of Pharmacy, Semmelweis University Medical School, Budapest, Hungary UNIVERSITATST BIBL1OTHEK ELSEVIER SCIENTIFIC PUBLISHING COMPANY Amsterdam Oxford New York 1983

2 CONTENTS Preface INTRODUCTION Problems and tendencies in contemporary steroid analysis Literature sources on analysis of steroids Scope and limitations 17 References SEX HORMONES AND RELATED MATERIALS Fundamentals of the chemistry of sex hormones Methods of analysis of sex hormones 2* Ultraviolet spectrophotometric methods based on natural absorption 24 a,f)-unsaturated ketones 24 3,5-Dienes 28 Phenolic ring A Ultraviolet spectrophotometric and colorimetric methods based on chemical reactions 32 Determination of keto groups 32 Phenolic ring A 40 Miscellaneous Fluorimetry 45 Methods based on native fluorescence 45 Methods based on acid-induced fluorescence 47 Miscellaneous fluorimetric methods Infrared spectrophotometry NMR spectrometry Polarography Titrimetric methods Gravimetric methods Column chromatography Thin-layer chromatography 68 Separation and detection problems 68 Quantitative analysis using spot elution 71 In situ methods of quantitation Gas chromatography 75 Packed columns 75 Capillary columns 78

3 Detectors 82 Problems of derivatization 84 Choice of derivates 86 Preparation of samples for quantitative analysis 94 Practical applications High-performance liquid chromatography 99 The chromatographic system 100 Detectors 108 Derivatization methods 110 Practical applications Radioimmunoassay and other radioisotope methods 116 Radioactive tracer methods 116 Double isotope derivative method 117 Competitive protein binding method 118 Radioimmunoassay Enzymatic methods 128 Colorimetric and fiuorimetric methods 128 Radioenzymatic methods 130 Enzyme immunoassay Mass spectrometry and related techniques 134 General aspects 134 Mass fragmentography Miscellaneous methods Problems in the analysis of sex hormones Purity control of bulk hormones Determination of sex hormones in pharmaceutical preparations Determination of sex hormones and their metabolites in biological samples 148 General aspects 148 Extraction procedures 149 Chromatographic separation and purification of extracts 150.Cleavage of conjugates 151 Spectroscopic methods of quantitation. 152 Chromatographic methods of quantitation 153 Radioanalytical methods of quantitation 154 Some schemes of complex analytical procedures Determination of sex hormones and anabolic agents in foods 159 References CORTICOSTEROIDS Fundamentals of the chemistry of corticosteroids Methods of analysis of corticosteroids Ultraviolet spectrophotometric and colorimetric methods based on chemical reactions 187 The tetrazolium method 187 The Porter-Silber method 189 Other methods Fluorimetry Polarography Column chromatography Paper and thin-layer chromatography 201 Paper chromatography 201

4 TLC separation and detection problems 202 Quantitative analysis Gas chromatography 205 The thermal instability of the 17-side-chain 205 Derivatization methods High-performance liquid chromatography Radioimmunoassay and other radioisotope methods 221 Competitive protein binding method 222 Radioimmunoassay Enzymatic methods 227 Photometric and fluorimetric methods 227 Enzyme immunoassay Mass spectrometry and related techniques Miscellaneous methods Problems in the analysis of corticosteroids Purity control of bulk corticosteroids Determination of corticosteroids in pharmaceutical preparations Determination of corticosteroids and their metabolites in biological samples 234 Preparation of samples for quantitative analysis 234 Quantitative determination Determination of corticosteroids in foods 237 References CHOLESTEROL AND RELATED STEROLS Fundamentals of the chemistry of sterols Methods for analysis of sterols Spectropnoiometric and colorimetric methods Fluorimetry Digitonide precipitation methods Electroanalytical methods Column chromatography Thin-layer chromatography 257 Separation and detection problems 257 Quantitative analysis Gas chromatography, 261 Derivatization 261 Separation problems 264 Quantitative analysis High-performance liquid chromatography Enzymatic methods 268 General aspects 268 Colorimetric methods 270 Fluorimetric methods, 272 Electroanalytical methods Mass spectrometry and related techniques Miscellaneous methods Problems in the analysis of sterols Determination of cholesterol in blood serum and other biological samples Determination of cholesterol in the raw materials of its production (wool. wax, spinal cord) 280

5 4.3.3 Determination of sterols in plants Determination of sterols in foods 281 References VITAMINS D AND RELATED MATERIALS Fundamentals of the chemistry of vitamins D Methods of vitamin D analysis Ultraviolet spectrophotometric methods " Colorimetric methods Infrared spectrophotometry NMR spectrometry Polarography Column chromatography Thin-layer chromatography Gas chromatography High-performance liquid chromatography Competitive protein binding method and other radioisotope techniques Mass spectrometry and related techniques Miscellaneous methods Problems in the analysis of vitamins D Purity control of crystalline vitamins D Analysis of vitamin D resins and other irradiation mixtures Determination of vitamins D in pharmaceutical preparations Determination of vitamins D in foods Determination of vitamins D and their metabolites in blood 331 References BILE ACIDS Fundamentals of the chemistry of bile acids Methods of analysis of bile acids Colorimetric methods Fluorimetry Column chromatography Thin-layer chromatography " 344 Separation of bile acids and conjugates 345 TLC fractionation of bile acids 346 Quantitative analysis using spot elution 346 In situ methods of quantitation Gas chromatography High-performance liquid chromatography Enzymatic methods Mass spectrometry and related techniques Radioimmunoassay Miscellaneous methods Problems in the analysis of bile acids Determination of bile acids and conjugates in biological samples Analysis of bulk bile acids and pharmaceutical preparations made therefrom 365 References 366

6 7. CARDIAC GLYCOSIDES Fundamentals of the chemistry of cardiac glycosides Methods of analysis of cardiac glycosides Ultraviolet spectrophotometry Colorimetric method" Fluorimetry Column chromatography Thin-layer chromatography 382 Separation and detection problems 384 Quantitative analysis using spot elution 384 In situ methods of quantitation Gas chromatography High-performance liquid chromatography Radioimmunoassay and enzyme immunoassay 393 Radioimmunoassay 396 Enzyme immunoassay Miscellaneous methods Problems in the analysis of cardiac glycosides Purity control in isolated cardiac glycosides Determination of cardiac glycosides in pharmaceutical preparations Determination of cardiac glycosides in plants Determination of cardiac glycosides and their metabolites in body fluids 403 References 8. DIOSGENIN AND RELATED SAPOGENINS Fundamentals of the chemistry of sapogenins Methods of analysis of sapogenins Colorimetric methods Infrared spectrophotometry Thin-layer chromatography Column and high-performance column chromatography Gas chromatography Miscellaneous methods Problems in the analysis of sapogenins Purity control of bulk sapogenins Determination of sapogenins in plants 418 References MISCELLANEOUS STEROIDS Ecdysones Solasodine and related alkaloids Pancuronium bromide and related quaternary ammonium compounds 429 References 431 Subject index 435

Prostaglandins and related substances a practical approach

Prostaglandins and related substances a practical approach O Prostaglandins and related substances a practical approach Edited by c Benedetto Institute of Obstetrics and Gynecology, Universita di Torino, Via Ventimiglia 3, Torino 10126, Italy R C McDonald-Gibson

More information

Water-soluble Vitamin Assays in Human Nutrition

Water-soluble Vitamin Assays in Human Nutrition Water-soluble Vitamin Assays in Human Nutrition Water-soluble Vitamin Assays in Human Nutrition G.F.M. Ball SPRINGER-SCIENCE+BUSINESS MEDIA, B.V. First edition 1994 1994 Springer Science+Business Media

More information

Gas Chromatography/ Mass Spectrometry

Gas Chromatography/ Mass Spectrometry Gas Chromatography/ Mass Spectrometry Edited by H.F. Linskens and J.F. Jackson Contributors R.S. Bandurski G. Combaut A.Ehmann P.Hedden B.Janistyn H.Kameoka H.Kodama D.V.Lynch J.K.MacLeod H.Nyberg L.M.S.Palni

More information

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page:

Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry Journal home page: Banu Rekha J and Jaykar. / Asian Journal of Pharmaceutical Analysis and Medicinal Chemistry. 3(2), 2015, 76-81. Research Article CODEN: AJPAD7 ISSN: 2321-0923 Asian Journal of Pharmaceutical Analysis and

More information

BIOCHEMISTRY & MEDICINE:

BIOCHEMISTRY & MEDICINE: BIOCHEMISTRY & MEDICINE: INTRODUCTION Biochemistry can be defined as the science of the chemical basis of life (Gk bios "life"). The cell is the structural unit of living systems. Thus, biochemistry can

More information

Metabolomics: quantifying the phenotype

Metabolomics: quantifying the phenotype Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems

More information

Lipid Analysis. Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th Introduction to lipid structures

Lipid Analysis. Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th Introduction to lipid structures Lipid Analysis Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th 2005 Introduction to lipid structures Fatty acids Acylglycerols Glycerophospholipids Sterols Strategies involved in lipid

More information

Good pharmacopoeial practices: Chapter on monographs on herbal medicines

Good pharmacopoeial practices: Chapter on monographs on herbal medicines Annex 7 Good pharmacopoeial practices: Chapter on monographs on herbal medicines Background Following the fiftieth meeting of the WHO Expert Committee on Specifications for Pharmaceutical Preparations,

More information

Analytical Chemistry of Foods

Analytical Chemistry of Foods Analytical Chemistry of Foods Analytical Chemistry of Foods c. s. JAMES Seale-Hayne Faculty of Agriculture, Food and Land Use Department of Agriculture and Food Studies University of Plymouth SPRINGER-SCIENCE+BUSINESS

More information

SECTION 1 LEARNING OBJECTIVES:

SECTION 1 LEARNING OBJECTIVES: CHAPTER 5 DRUGS SECTION 1 LEARNING OBJECTIVES: 1. Define psychological and physical dependence. 2. Name and classify the commonly abused drugs. 3. Describe the tendency to develop psychological and physical

More information

Savesta Herbals is engaged in manufacturing

Savesta Herbals is engaged in manufacturing Savesta Herbals is engaged in manufacturing standardized herbal extracts that synergistically combine the ancient wisdom of Ayurveda with the modern production and QC methods. Our state-of- the- art production

More information

Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND. Bridgwater, England LIPIDOMIC ANALYSIS. Fourth Edition. Invergowrie, Dundee, Scotland

Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND. Bridgwater, England LIPIDOMIC ANALYSIS. Fourth Edition. Invergowrie, Dundee, Scotland Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND LIPIDOMIC ANALYSIS Fourth Edition WILLIAM W.CHRISTIE MRS Lipid Analysis Unit, Scottish Crop Research Institute, Dundee, Scotland Invergowrie, and

More information

Hawler Medical University College of Pharmacy Dept. of Pharmacognosy Course Book Pharmacognosy I and II, 3 rd year

Hawler Medical University College of Pharmacy Dept. of Pharmacognosy Course Book Pharmacognosy I and II, 3 rd year Hawler Medical University College of Pharmacy Dept. of Pharmacognosy Course Book Pharmacognosy and, 3 rd year Course Coordinator: Dr. Alaadin M. Naqishbandi Assistant Professor 014-015 Course Coordinator

More information

CH 3. Lipids CHAPTER SUMMARY

CH 3. Lipids CHAPTER SUMMARY H 3 C H 3 C 15 H 3 C H Views of Cholesterol APTER SUMMARY 15.1 The Nature of can best be defined as biomolecules which are soluble to a great extent in solvents. In contrast to carbohydrates, proteins

More information

Determination of Tanninoids. Analytical Pharmacognosy

Determination of Tanninoids. Analytical Pharmacognosy QUIZ If the manager of a phytopharmaceutical industry wish to buy one chromatographic equipment, which one you recommend, HPLC or TLC densitometer. What are the reasons to support your recommendation.

More information

Lipids are broadly classified in to simple, complex and derived, which are further subdivided into different groups.

Lipids are broadly classified in to simple, complex and derived, which are further subdivided into different groups. Paper No. 01 Paper Title: Food Chemistry Module -9: Classification of lipids Lipids are organic substances which are insoluble in water and soluble in organic solvents. Lipids are not polymers and exist

More information

TENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010)

TENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010) June 2010 TENOFOVIR TABLETS: Final text for addition to The International Pharmacopoeia (June 2010) This monograph was adopted at the Forty-fourth WHO Expert Committee on Specifications for Pharmaceutical

More information

ARTENIMOLUM ARTENIMOL. Adopted revised text for addition to The International Pharmacopoeia

ARTENIMOLUM ARTENIMOL. Adopted revised text for addition to The International Pharmacopoeia February 2012 ARTENIMOLUM ARTENIMOL Adopted revised text for addition to The International Pharmacopoeia This monograph was adopted at the Forty-sixth WHO Expert Committee on Specifications for Pharmaceutical

More information

Title Revision n date

Title Revision n date A. THIN LAYER CHROMATOGRAPHIC TECHNIQUE (TLC) 1. SCOPE The method describes the identification of hydrocortisone acetate, dexamethasone, betamethasone, betamethasone 17-valerate and triamcinolone acetonide

More information

CONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management

CONTENT. i iv ix. SVKM s NMIMS, School of Pharmacy and Technology Management CONTENT Chapter No. Title Page No. Abbreviations List of Figures List of Tables 1 Introduction 1 1.1 Practical aspects 4 1.2 Stability-indicating assay method (SIAM) 5 1.3 Regulatory aspects 6 1.4 Techniques

More information

Heparin Sodium ヘパリンナトリウム

Heparin Sodium ヘパリンナトリウム Heparin Sodium ヘパリンナトリウム Add the following next to Description: Identification Dissolve 1 mg each of Heparin Sodium and Heparin Sodium Reference Standard for physicochemical test in 1 ml of water, and

More information

Isolation, Separation, and Characterization of Organic Acids*

Isolation, Separation, and Characterization of Organic Acids* In Dashek, William V., ed. Methods in plant biochemistry and molecular biology. Boca Raton, FL: CRC Press: pp. 107-113. Chapter 9.1997. Isolation, Separation, and Characterization of Organic Acids* William

More information

Composition and Structure of Bovine Milk Lipids Introduction p. 1 Fatty Acids p. 3 Origins of the Fatty Acids p. 4 Saturated Fatty Acids p.

Composition and Structure of Bovine Milk Lipids Introduction p. 1 Fatty Acids p. 3 Origins of the Fatty Acids p. 4 Saturated Fatty Acids p. Composition and Structure of Bovine Milk Lipids Introduction p. 1 Fatty Acids p. 3 Origins of the Fatty Acids p. 4 Saturated Fatty Acids p. 5 Cis-unsaturated Fatty Acids p. 5 Trans-unsaturated Fatty Acids

More information

Choosing the metabolomics platform

Choosing the metabolomics platform Choosing the metabolomics platform Stephen Barnes, PhD Department of Pharmacology & Toxicology University of Alabama at Birmingham sbarnes@uab.edu Challenges Unlike DNA, RNA and proteins, the metabolome

More information

Tenofovir disoproxil fumarate (Tenofoviri disoproxili fumaras)

Tenofovir disoproxil fumarate (Tenofoviri disoproxili fumaras) C 19 H 30 N 5 O 10 P. C 4 H 4 O 4 Relative molecular mass. 635.5. Chemical names. bis(1-methylethyl) 5-{[(1R)-2-(6-amino-9H-purin-9-yl)-1-methylethoxy]methyl}-5-oxo-2,4,6,8-tetraoxa-5-λ 5 - phosphanonanedioate

More information

Organic Chemistry Laboratory Fall Lecture 3 Gas Chromatography and Mass Spectrometry June

Organic Chemistry Laboratory Fall Lecture 3 Gas Chromatography and Mass Spectrometry June 344 Organic Chemistry Laboratory Fall 2013 Lecture 3 Gas Chromatography and Mass Spectrometry June 19 2013 Chromatography Chromatography separation of a mixture into individual components Paper, Column,

More information

Chromatography Vacuum Ultraviolet Spectroscopy

Chromatography Vacuum Ultraviolet Spectroscopy Application Note Differentiation and Determination Differentiation and Determination of Fatty Acid Methyl of Fatty Esters Acid by Gas Methyl Chromatography Esters by Vacuum Gas Ultraviolet Spectroscopy

More information

CAMAG TLC-MS INTERFACE

CAMAG TLC-MS INTERFACE CAMAG TLC-MS INTERFACE 93.1 249.2 40 30 97.1 20 10 250.2 0 200 400 m/z WORLD LEADER IN PLANAR-CHROMATOGRAPHY Identification and elucidation of unknown substances by hyphenation of TLC / HPTLC and MS The

More information

Comparison of Water adsorption characteristics of oligo and polysaccharides of α-glucose studied by Near Infrared Spectroscopy Alfred A.

Comparison of Water adsorption characteristics of oligo and polysaccharides of α-glucose studied by Near Infrared Spectroscopy Alfred A. Comparison of Water adsorption characteristics of oligo and polysaccharides of α-glucose studied by Near Infrared Spectroscopy Alfred A. Christy, Department of Science, Faculty of Engineering and Science,

More information

CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS

CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) ANALYSIS OF PHYTOCHEMICAL CONSTITUENTS OF M. ROXBURGHIANUS AND P. FRATERNUS PLANT EXTRACTS CHAPTER 8: HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

More information

Fractionation of fecal neutral steroids by high performance liquid chromatography

Fractionation of fecal neutral steroids by high performance liquid chromatography Fractionation of fecal neutral steroids by high performance liquid chromatography Evelyn M. Jackson,"* Cheryl A. Kloss,* Susan T. Weintraub,** and Glen E. Matt*'** Cardiopulmonary Department, Southwest

More information

ARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) ARTESUNATI COMPRESSI ARTESUNATE TABLETS

ARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) ARTESUNATI COMPRESSI ARTESUNATE TABLETS December 2009 ARTESUNATE TABLETS: Final text for revision of The International Pharmacopoeia (December 2009) This monograph was adopted at the Forty-fourth WHO Expert Committee on Specifications for Pharmaceutical

More information

Prerequisites Protein purification techniques and protein analytical methods. Basic enzyme kinetics.

Prerequisites Protein purification techniques and protein analytical methods. Basic enzyme kinetics. Case 19 Purification of Rat Kidney Sphingosine Kinase Focus concept The purification and kinetic analysis of an enzyme that produces a product important in cell survival is the focus of this study. Prerequisites

More information

2. Selection criteria for substances of herbal origin relevant for standardization and quality control of herbal medicines 77

2. Selection criteria for substances of herbal origin relevant for standardization and quality control of herbal medicines 77 Annex 1 WHO guidelines for selecting marker substances of herbal origin for quality control of herbal medicines 1. Introduction 72 1.1 Background 72 1.1.1 Preparation of the document 73 1.2 Objectives

More information

ANALYTICAL SCIENCES OCTOBER 2018, VOL The Japan Society for Analytical Chemistry

ANALYTICAL SCIENCES OCTOBER 2018, VOL The Japan Society for Analytical Chemistry ANALYTICAL SCIENCES OCTOBER 2018, VOL. 34 1195 2018 The Japan Society for Analytical Chemistry Process for the Purification of cis-p-coumaric Acid by Cellulose Column Chromatography after the Treatment

More information

Characterisation of a Cu selective resin for use in the production of Cu isotopes

Characterisation of a Cu selective resin for use in the production of Cu isotopes Characterisation of a Cu selective resin for use in the production of Cu isotopes Resin characterisation Selectivity Interferences with Ni or Zn Column Experiments Optimisation Simulated targets Decontamination

More information

Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application

Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application Small Scale Preparative Isolation of Corticosteroid Degradation Products Using Mass-Based Fraction Collection Application Pharmaceutical Author Cliff Woodward Agilent Technologies, Inc. 285 Centerville

More information

A Novel HILIC Column for High Speed N-linked Glycan Analysis

A Novel HILIC Column for High Speed N-linked Glycan Analysis A Novel HILIC for High Speed N-linked Glycan Analysis Application Note Biotherapeutics and Biosimilars Authors James Martosella, Oscar Potter, Danny Mancheno, and Jia Liu Agilent Technologies, Inc. Introduction

More information

This annex is valid from: to Replaces annex dated: Location(s) where activities are performed under accreditation

This annex is valid from: to Replaces annex dated: Location(s) where activities are performed under accreditation Stichting ageningen Research Location(s) where activities are performed under accreditation Head Office Akkermaalsbos 2 6708 B ageningen Nederland Location Abbreviation/ location code Akkermaalsbos 2 6708

More information

Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica

Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica Residue Monograph prepared by the meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA), 82 nd meeting 2016 Rebaudioside a From Multiple Gene Donors Expressed in Yarrowia Lipolytica This

More information

COMPARATIVE INVESTIGATION OF FOOD SUPPLEMENTS CONTAINING ASCORBIC ACID

COMPARATIVE INVESTIGATION OF FOOD SUPPLEMENTS CONTAINING ASCORBIC ACID PHARMACIA, vol. 60, No. 4/2013 37 COMPARATIVE INVESTIGATION OF FOOD SUPPLEMENTS CONTAINING ASCORBIC ACID Danka Obreshkova and Boyka Tsvetkova Medical University Sofia, Faculty of Pharmacy, Dept. of Pharmaceutical

More information

Determination of Triglycerides and Waxes in Food Products Using Cool On-Column Injection and the MET- Biodiesel Capillary Column

Determination of Triglycerides and Waxes in Food Products Using Cool On-Column Injection and the MET- Biodiesel Capillary Column Page 1 of 6 Page 1 of 6 Return to Web Version Determination of Triglycerides and Waxes in Food Products Using Cool On-Column Injection and the MET- Biodiesel Capillary Column By: Michael D. Buchanan, Reporter

More information

Journal of Global Trends in Pharmaceutical Sciences

Journal of Global Trends in Pharmaceutical Sciences An Elsevier Indexed Journal ISSN-2230-7346 Journal of Global Trends in Pharmaceutical Sciences METHOD DEVELOPMENT AND VALIDATION OF ANASTROZOLE IN TABLET DOSAGE FORM BY RP-HPLC METHOD Divya T 1*, Pavani

More information

High-Performance Liquid Chromatographic Determination of Vitamin D3 in Fish Liver Oils and Eel Body Oils

High-Performance Liquid Chromatographic Determination of Vitamin D3 in Fish Liver Oils and Eel Body Oils J. Nutr. Sci. VitaminoL, 30, 421-430, 1984 High-Performance Liquid Chromatographic Determination of Vitamin D3 in Fish Liver Oils and Eel Body Oils Atsuko TAKEUCHI, Toshio OKANO, Mani AYAME, Harumi YOSHIKAWA,

More information

Facile Isolation of Carotenoid Antioxidants from Solanum lycopersicum using Flash Chromatography

Facile Isolation of Carotenoid Antioxidants from Solanum lycopersicum using Flash Chromatography Facile Isolation of Carotenoid Antioxidants from Solanum lycopersicum using Flash Chromatography Jack E. Silver, jsilver@teledyne.com, Paul Bellinghausen, Nancy Fowler, and Ruth Pipes, Teledyne Isco, Inc.,

More information

Org/Biochem Final Lec Form, Spring 2012 Page 1 of 6

Org/Biochem Final Lec Form, Spring 2012 Page 1 of 6 Page 1 of 6 Missing Complete Protein and Question #45 Key Terms: Fill in the blank in the following 25 statements with one of the key terms in the table. Each key term may only be used once. Print legibly.

More information

Special Considerations for Tracers Based on Proteins or Protein Fragments

Special Considerations for Tracers Based on Proteins or Protein Fragments Special Considerations for Tracers Based on Proteins or Protein Fragments 10 June, 2017 Serge K. Lyashchenko, Pharm.D. Assistant Attending, Memorial Hospital, Memorial Sloan Kettering Cancer Center Assistant

More information

Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method

Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method Asian Journal of Chemistry Vol. 22, No. 5 (2010), 3369-3375 Simultaneous Determination of Halobetasol and Salicylic Acid Related Substances by Reversed Phase High Performance Liquid Chromatographic Method

More information

INTERNATIONAL PHARMACOPOEIA MONOGRAPH ON LAMIVUDINE TABLETS

INTERNATIONAL PHARMACOPOEIA MONOGRAPH ON LAMIVUDINE TABLETS RESTRICTED INTERNATIONAL PHARMACOPOEIA MONOGRAPH ON LAMIVUDINE TABLETS DRAFT FOR COMMENT Please address any comments you may have on this document, by 12 July 2006, to Dr S. Kopp, Quality Assurance and

More information

Identification of Aromatic Fatty Acid Ethyl Esters

Identification of Aromatic Fatty Acid Ethyl Esters Chapter 3.2 Identification of Aromatic Fatty Acid Ethyl Esters The only use of gas chromatography is not sufficient to determine which compounds are eluting from the catalytic bed. At the beginning of

More information

Draft monograph for inclusion in. The International Pharmacopoeia. Dextromethorphani solutionum peroralum - Dextromethorphan oral solution

Draft monograph for inclusion in. The International Pharmacopoeia. Dextromethorphani solutionum peroralum - Dextromethorphan oral solution August 2015 Draft document for comment 1 2 3 4 5 6 Draft monograph for inclusion in The International Pharmacopoeia Dextromethorphani solutionum peroralum - Dextromethorphan oral solution (August 2015)

More information

Test Bank for Lehninger Principles of Biochemistry 5th Edition by Nelson

Test Bank for Lehninger Principles of Biochemistry 5th Edition by Nelson Test Bank for Lehninger Principles of Biochemistry 5th Edition by Nelson Link download full: http://testbankair.com/download/test-bank-forlehninger-principles-of-biochemistry-5th-edition-by-nelson/ Chapter

More information

Chukvelutins A-C, 16-norphragmalin limonoids with unprecedented skeletons from Chukrasia tabularis var. velutina

Chukvelutins A-C, 16-norphragmalin limonoids with unprecedented skeletons from Chukrasia tabularis var. velutina Chukvelutins A-C, 16-norphragmalin limonoids with unprecedented skeletons from Chukrasia tabularis var. velutina Jun Luo, Jun-Song Wang, Jian-Guang Luo, Xiao-Bing Wang, and Ling-Yi Kong* Department of

More information

DiscovIR-LC. Application Note 026 May 2008 READING TEA LEAVES SUMMARY INTRODUCTION

DiscovIR-LC. Application Note 026 May 2008 READING TEA LEAVES SUMMARY INTRODUCTION TM DiscovIR-LC Deposition and Detection System Application Note 026 May 2008 READING TEA LEAVES The DiscovIR-LC is a powerful new tool for materials analysis. When connected to the outlet of an LC column,

More information

High Performance Liquid Chromatography

High Performance Liquid Chromatography High Performance Liquid Chromatography 1 / 6 2 / 6 Right here, we have countless ebook and collections to check out. We additionally provide variant types and afterward type of the books to browse. The

More information

Lipid Chemistry. Presented By. Ayman Elsamanoudy Salwa Abo El-khair

Lipid Chemistry. Presented By. Ayman Elsamanoudy Salwa Abo El-khair Lipid Chemistry Presented By Ayman Elsamanoudy Salwa Abo El-khair 6 1. By the end of this chapter the student should be able to: define lipids. describe the biological importance of lipids. point out basic

More information

A comparison study of the analysis of volatile organic acids and fatty acids

A comparison study of the analysis of volatile organic acids and fatty acids Application Note Food Testing A comparison study of the analysis of volatile organic acids and fatty acids Using DB-FATWAX Ultra Inert and other WAX GC columns Author Yun Zou Agilent Technologies (Shanghai)

More information

SYNOPSIS STUDIES ON THE PREPARATION AND CHARACTERISATION OF PROTEIN HYDROLYSATES FROM GROUNDNUT AND SOYBEAN ISOLATES

SYNOPSIS STUDIES ON THE PREPARATION AND CHARACTERISATION OF PROTEIN HYDROLYSATES FROM GROUNDNUT AND SOYBEAN ISOLATES 1 SYNOPSIS STUDIES ON THE PREPARATION AND CHARACTERISATION OF PROTEIN HYDROLYSATES FROM GROUNDNUT AND SOYBEAN ISOLATES Proteins are important in food processing and food product development, as they are

More information

DRAFT PROPOSAL FOR THE INTERNATIONAL PHARMACOPOEIA: CARBAMAZEPINI COMPRESSI - CARBAMAZEPINE TABLETS

DRAFT PROPOSAL FOR THE INTERNATIONAL PHARMACOPOEIA: CARBAMAZEPINI COMPRESSI - CARBAMAZEPINE TABLETS December 2015 Draft document for comment 1 2 3 4 5 6 DRAFT PROPOSAL FOR THE INTERNATIONAL PHARMACOPOEIA: CARBAMAZEPINI COMPRESSI - CARBAMAZEPINE TABLETS (December 2015) REVISED DRAFT FOR COMMENT Should

More information

Lec 4a- BPK 110 Human Nutrition: Current Iss.

Lec 4a- BPK 110 Human Nutrition: Current Iss. Lec 4a- BPK 110 Human Nutrition: Current Iss. TOPICS FOR Lec 4a: 1. Introduction to Lipids 2. Lipid Structure 3. Saturated vs. Unsaturated Fatty Acid Chains 4. Phospholipids and Sterols (Other Lipids)

More information

Title Spot Test Method Convenient in Column Chromatography for Det Author(s) Morita, Shigeru; Hanai, Tetsuya Citation Bulletin of the Institute for Chemi University (1975), 53(3): 279-283 Issue Date 1975-09-16

More information

RITONAVIRI COMPRESSI RITONAVIR TABLETS. Final text for addition to The International Pharmacopoeia (July 2012)

RITONAVIRI COMPRESSI RITONAVIR TABLETS. Final text for addition to The International Pharmacopoeia (July 2012) July 2012 RITONAVIRI COMPRESSI RITONAVIR TABLETS Final text for addition to The International Pharmacopoeia (July 2012) This monograph was adopted at the Forty-sixth WHO Expert Committee on Specifications

More information

The Third Department of Internal Medicine, University of Tokyo Faculty of Medicine, Hongo, Tokyo 113

The Third Department of Internal Medicine, University of Tokyo Faculty of Medicine, Hongo, Tokyo 113 Endocrinol. Japon. 1974, 21 (2), 115 ` 119 A Radioimmunoassay for Serum Dehydroepiandrosterone HISAHIKO SEKIHARA, TOHRU YAMAJI, NAKAAKI OHSAWA AND HIROSHI IBAYASHI * The Third Department of Internal Medicine,

More information

601 S. College Avenue Kaitlin L. Siela Columbia, MO Telephone: (816)

601 S. College Avenue Kaitlin L. Siela Columbia, MO Telephone: (816) Jane M. Jackson Department of Chemistry Telephone: (816) 695-8701 University of Missouri Columbia Email: jmj9q7@mail.missouri.edu 105 Chemistry Building 601 S. College Avenue Kaitlin L. Siela Columbia,

More information

This annex is valid from: to Replaces annex dated: Location(s) where activities are performed under accreditation

This annex is valid from: to Replaces annex dated: Location(s) where activities are performed under accreditation Stichting ageningen Research Location(s) where activities are performed under accreditation Head Office Akkermaalsbos 2 6708 B ageningen The Netherlands Location Abbreviation/ location code Akkermaalsbos

More information

MALDI-TOF. Introduction. Schematic and Theory of MALDI

MALDI-TOF. Introduction. Schematic and Theory of MALDI MALDI-TOF Proteins and peptides have been characterized by high pressure liquid chromatography (HPLC) or SDS PAGE by generating peptide maps. These peptide maps have been used as fingerprints of protein

More information

Electrolyte Analyzer with Ion-Selective Electrode and Blood Gas analyzer

Electrolyte Analyzer with Ion-Selective Electrode and Blood Gas analyzer Biochemistry & Fundamental Reference Laboratory, Yokohama Our Head Office and Biochemistry Reference Laboratory moved to Yokohama with aims to have further development on the 21 st September, 2018. [Reference

More information

Lipids. Lipids. Jiří Jonák and Lenka Fialová Institute of Medical Biochemistry, 1st Medical Faculty of the Charles University, Prague

Lipids. Lipids. Jiří Jonák and Lenka Fialová Institute of Medical Biochemistry, 1st Medical Faculty of the Charles University, Prague Lipids Jiří Jonák and Lenka Fialová Institute of Medical Biochemistry, 1st Medical Faculty of the Charles University, Prague Lipids 1. General introduction 2. Nomenclature of fatty acids 3. Degradation

More information

Chemistry 1120 Exam 2 Study Guide

Chemistry 1120 Exam 2 Study Guide Chemistry 1120 Exam 2 Study Guide Chapter 6 6.1 Know amines are derivatives of ammonia, which is not an amine. Classify amines as primary, secondary or tertiary. Master Tutor Section 6.1 Review Section

More information

SAURASHTRA UNIVERSITY

SAURASHTRA UNIVERSITY SAURASHTRA UNIVERSITY COURSE STRUCTURE & SYLLABUS FOR UNDERGRADUATE PROGRAMME IN BIOCHEMISTRY 3 rd and 4 th Semester CORE COURSES UNDER CHOICE BASED CREDIT SEMESTER SYSTEM w.e.f June, 2017. COURSE STRUCTURE

More information

Detection of Lipid Peroxidation Products From Free Radical and Enzymatic Processes. Jason D. Morrow M.D. Vanderbilt University School of Medicine

Detection of Lipid Peroxidation Products From Free Radical and Enzymatic Processes. Jason D. Morrow M.D. Vanderbilt University School of Medicine Detection of Lipid Peroxidation Products From Free Radical and Enzymatic Processes Jason D. Morrow M.D. Vanderbilt University School of Medicine Question? Which one of the following is the most accurate

More information

Nutrition is the study of the nutrients in food and how they nourish the body.

Nutrition is the study of the nutrients in food and how they nourish the body. Chapter 2 Nutrients Copyright 2011 by the National Restaurant Association Educational Foundation (NRAEF) and published by Pearson Education, Inc. All rights reserved. People need certain nutrients on a

More information

You must answer FOUR of the SIX questions. Use a SEPARATE answer book for EACH question.

You must answer FOUR of the SIX questions. Use a SEPARATE answer book for EACH question. UNIVERSITY OF EAST ANGLIA School of Pharmacy Main Series UG Examination 2016-17 DRUG DESIGN AND MECHANISMS OF DRUG ACTION PHA-5001Y Time allowed: 2 hours You must answer FOUR of the SIX questions. Use

More information

Analysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column

Analysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column Application Note Food Analysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column Authors Ingrid Van Der Meer, Yun Zou, and Gustavo Serrano Agilent

More information

In vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae)

In vitro antioxidant activity of a flavonoid compound isolated from methanolic extract of Helianthus annuus leaves (Asteraceae) Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2017, 9 [6]:28-34 [http://scholarsresearchlibrary.com/archive.html] ISSN 0975-5071 USA CODEN: DPLEB4

More information

Glycosylation analysis of blood plasma proteins

Glycosylation analysis of blood plasma proteins Glycosylation analysis of blood plasma proteins Thesis booklet Eszter Tóth Doctoral School of Pharmaceutical Sciences Semmelweis University Supervisor: Károly Vékey DSc Official reviewers: Borbála Dalmadiné

More information

Lipids fatty, oily, or waxy hydrophobic organic compounds.

Lipids fatty, oily, or waxy hydrophobic organic compounds. Lipids Lipids Lipids fatty, oily, or waxy hydrophobic organic compounds. u long hydrocarbon chain u composed of CHO Diverse group u fats u oils u waxes u steroids Do not form polymers u big molecules made

More information

TENOFOVIRI DISOPROXILIS FUMARAS TENOFOVIR DISOPROXIL FUMARATE

TENOFOVIRI DISOPROXILIS FUMARAS TENOFOVIR DISOPROXIL FUMARATE August 2009 RESTRICTED TENOFOVIRI DISOPROXILIS FUMARAS TENOFOVIR DISOPROXIL FUMARATE DRAFT PROPOSAL FOR THE INTERNATIONAL PHARMACOPOEIA FOR COMMENT Please send any comments on this document to Dr S. Kopp

More information

Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High-performance Normal Phase Partition Chromatography (2) Table of Contents

Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High-performance Normal Phase Partition Chromatography (2) Table of Contents No. 079 SEPARATION REPORT Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High-performance Normal Phase Partition Chromatography (2) Table of Contents 1. Introduction 1 2. Comparison

More information

Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts

Study of Phytochemical Screening and Antimicrobial Activity of Citrus aurantifolia Seed Extracts American Journal of Analytical Chemistry, 2016, 7, 254-259 Published Online March 2016 in SciRes. http://www.scirp.org/journal/ajac http://dx.doi.org/10.4236/ajac.2016.73022 Study of Phytochemical Screening

More information

Sepax Technologies, Inc.

Sepax Technologies, Inc. Sepax Technologies, Inc. Sepax Technologies, Inc. develops and manufactures products in the area of chemical and biological separations, biosurfaces and proteomics. Sepax product portfolio includes ) liquid

More information

Delineation of the Role of Glycosylation in the Cytotoxic Properties of Quercetin using Novel Assays in Living Vertebrates

Delineation of the Role of Glycosylation in the Cytotoxic Properties of Quercetin using Novel Assays in Living Vertebrates Delineation of the Role of Glycosylation in the Cytotoxic Properties of Quercetin using Novel Assays in Living Vertebrates Si-Hwan Park,, Hyun Jung Kim,, Soon-Ho Yim, Ah-Ra Kim, Nisha Tyagi, Haihong Shen,

More information

Sphingolipids. Sphingolipids are an additional type of membrane lipids, after glycerophospholipids, galactolipids and sulfolipids

Sphingolipids. Sphingolipids are an additional type of membrane lipids, after glycerophospholipids, galactolipids and sulfolipids Lipids 2 Steven E. Massey, Ph.D. Assistant Professor of Bioinformatics Department of Biology and Environmental Sciences University of Puerto Rico Río Piedras Office & Lab: Bioinformatics Lab NCN#343B Tel:

More information

Case 19 Purification of Rat Kidney Sphingosine Kinase

Case 19 Purification of Rat Kidney Sphingosine Kinase Case 19 Purification of Rat Kidney Sphingosine Kinase Focus concept The purification and kinetic analysis of an enzyme that produces a product important in cell survival is the focus of this study. Prerequisites

More information

THIN LAYER CHROMATOGRAPHY

THIN LAYER CHROMATOGRAPHY THIN LAYER CHROMATOGRAPHY Thin layer chromatography is the best known technique of plant biochemistry. TLC is used for preliminary separation and determination of plant constituents. It is helpful for

More information

β-sitosterol-3-o-β-d-xylopyranosyl (1 4)-O-β-D- GLUCOPYRANOSIDE FROM THE SEEDS OF ZANTHOXYLUM HEMILTONIANUM WALL

β-sitosterol-3-o-β-d-xylopyranosyl (1 4)-O-β-D- GLUCOPYRANOSIDE FROM THE SEEDS OF ZANTHOXYLUM HEMILTONIANUM WALL Int. J. Chem. Sci.: 11(2), 201, 111-116 ISSN 0972-768X www.sadgurupublications.com β-sitsterl---β-d-xylpyransyl (1 4)--β-D- GLUCPYRANSIDE FRM TE SEEDS F ZANTXYLUM EMILTNIANUM WALL SANDYA RAJPT * Department

More information

Bile Acid Synthesis in Man: Metabolism of

Bile Acid Synthesis in Man: Metabolism of Bile Acid Synthesis in Man: Metabolism of 7a-Hydroxycholesterol-'4C and 26-Hydroxycholesterol-3H KAmR E. ANDERSON, ENGELINE KOK, and NORMAN B. JAVITT From the Gastroenterology Division, Department of Medicine,

More information

ANALYTICAL METHODS IN DETERMINING AFLATOXIN B1 IN FOOD

ANALYTICAL METHODS IN DETERMINING AFLATOXIN B1 IN FOOD 1 International Journal of Advance Research, IJOAR.org Volume 1, Issue 4, April 2013, ISSN 2320-9186 ANALYTICAL METHODS IN DETERMINING AFLATOXIN B1 IN FOOD MATERIALS Melvin Samuel. S * * School of Biosciences

More information

Improving Benzodiazepine Immunoassay Sensitivity by Rapid Glucuronide Hydrolysis Technology

Improving Benzodiazepine Immunoassay Sensitivity by Rapid Glucuronide Hydrolysis Technology Improving Benzodiazepine Immunoassay Sensitivity by Rapid Glucuronide Hydrolysis Technology Pongkwan (Nikki) Sitasuwan, Margarita Marinova, and L. Andrew Lee Integrated Micro-Chromatography Systems, LLC

More information

Clinical Chemistry

Clinical Chemistry Western Technical College 10513155 Clinical Chemistry Course Outcome Summary Course Information Description Career Cluster Instructional Level Total Credits 5.00 Introduces Clinical Chemistry techniques

More information

ISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL

ISOLATION AND CHARACTERIZATION OF HESPERIDIN FROM ORANGE PEEL Indo American Journal of Pharmaceutical Research, 2013 ISSN NO: 2231-6876 Journal home page: http:///index.php/en/ INDO AMERICAN JOURNAL OF PHARMACEUTICAL RESEARCH ISOLATION AND CHARACTERIZATION OF HESPERIDIN

More information

CORESTA Recommended Method No. 84

CORESTA Recommended Method No. 84 Cooperation Centre for Scientific Research Relative to Tobacco E-Vapour Sub-Group CORESTA Recommended Method No. 84 DETERMINATION OF GLYCERIN, PROPYLENE GLYCOL, WATER, AND NICOTINE IN THE AEROSOL OF E-CIGARETTES

More information

Syllabus for Training in Inborn Errors of Metabolism for Scientists and Medically Qualified Laboratory Staff

Syllabus for Training in Inborn Errors of Metabolism for Scientists and Medically Qualified Laboratory Staff Training Syllabus LABORATORY SYLLABUS Syllabus for Training in Inborn Errors of Metabolism for Scientists and Medically Qualified Laboratory Staff This syllabus is intended as a guide. Whilst the training

More information

DOWNLOAD OR READ : STEROLS AND BILE ACIDS PDF EBOOK EPUB MOBI

DOWNLOAD OR READ : STEROLS AND BILE ACIDS PDF EBOOK EPUB MOBI DOWNLOAD OR READ : STEROLS AND BILE ACIDS PDF EBOOK EPUB MOBI Page 1 Page 2 sterols and bile acids sterols and bile acids pdf sterols and bile acids Disturbances in their production can have deleterious

More information

BabyBio IMAC columns DATA SHEET DS

BabyBio IMAC columns DATA SHEET DS BabyBio IMAC columns DATA SHEET DS 45 655 010 BabyBio columns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged)

More information

Nutrients. Chapter 25 Nutrition, Metabolism, Temperature Regulation

Nutrients. Chapter 25 Nutrition, Metabolism, Temperature Regulation Chapter 25 Nutrition, Metabolism, Temperature Regulation 25-1 Nutrients Chemicals used by body to produce energy, provide building blocks or function in other chemical reactions Classes Carbohydrates,

More information

ABACAVIR SULFATE Proposal for revision of The International Pharmacopoeia (August 2012)

ABACAVIR SULFATE Proposal for revision of The International Pharmacopoeia (August 2012) August 2012 RESTRICTED ABACAVIR SULFATE Proposal for revision of The International Pharmacopoeia (August 2012) Draft for comment This document was provided by a quality control expert. Should you have

More information

Pantothenic Acid. Shang-Jing Pan, Ph.D. Abbott Nutrition Columbus, Ohio, USA

Pantothenic Acid. Shang-Jing Pan, Ph.D. Abbott Nutrition Columbus, Ohio, USA Pantothenic Acid Shang-Jing Pan, Ph.D. Abbott Nutrition Columbus, Ohio, USA Pantothenic acid- history Discovered by Roger J Williams in 1919. Isolated in 1933 by R. J. Williams. Named by Williams, meaning

More information

Matrix solid phase dispersion extraction for Tropane alkaloid detection in cultured and collected Hyoscyamus reticulatus plants

Matrix solid phase dispersion extraction for Tropane alkaloid detection in cultured and collected Hyoscyamus reticulatus plants ISSN 232-547 International Journal of Advanced Research (215), Volume 3, Issue 11, 78 82 Journal homepage: http://www.journalijar.com INTERNATIONAL JOURNAL OF ADVANCED RESEARCH RESEARCH ARTICLE Matrix

More information

WITH REGARD TO METHODS OF ANALYSIS FOR DETERMINATION OF THE FIBRE CONTENT DECLARED ON A LABEL

WITH REGARD TO METHODS OF ANALYSIS FOR DETERMINATION OF THE FIBRE CONTENT DECLARED ON A LABEL EUROPEAN COMMSSON HEALTH AND CONSUMERS DRECTORATE-GENERAL DECEMBER 2012 GUDANCE DOCUMENT FOR COMPETENT AUTHORTES FOR THE CONTROL OF COMPLANCE WTH EU LEGSLATON ON: Council Directive 90/496/EEC of 24 September

More information