Problem 2 (10 Points) The wild type sequence of the coding region of an mrna is shown below.
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5 Problem 2 (10 Points) The wild type sequence of the coding region of an mrna is shown below. 5 AUG ACC UGG AAU AAA UGA 3 Use that sequence to answer each of the questions below. a) What is the sequence of the wild type protein? (1 Point) b) A mutant version of this mrna was identified and the mutant protein was sequenced. The sequence of the mutant protein is Met Thr Trp Ser Lys. Assuming that the mutation is a single nucleotide substitution, insertion, or deletion, what is the sequence of the mutant mrna? (1 Point) What mutagen could have been used to induce this mutation? (2 Points) c) A second mutant version of this mrna was identified and the mutant protein was sequenced. The sequence of the mutant protein is Met Thr Trp Thr Lys. Assuming that the mutation is a single nucleotide substitution, insertion, or deletion, what is the sequence of the mutant mrna? (1 Point) What mutagen could have been used to induce this mutation? (2 Points) d) A third mutant version of this mrna was identified and the mutant protein was sequenced. The sequence of the mutant protein is Met Thr Trp Lys. Assuming that the mutation is a single nucleotide substitution, insertion, or deletion, what is the sequence of the mutant mrna? (1 Point) What mutagen could have been used to induce this mutation? (2 Points) 6
6 Problem 2 (10 Points) The wild type sequence of the coding region of an mrna is shown below. 5 AUG CCU UGG CAU AAA UGA 3 Use that sequence to answer each of the questions below. a) What is the sequence of the wild type protein? (1 Point) b) A mutant version of this mrna was identified and the mutant protein was sequenced. The sequence of the mutant protein is Met Pro Trp Tyr - Lys. Assuming that the mutation is a single nucleotide substitution, insertion, or deletion, what is the sequence of the mutant mrna? (1 Point) What mutagen could have been used to induce this mutation? (2 Points) c) A second mutant version of this mrna was identified and the mutant protein was sequenced. The sequence of the mutant protein is Met Pro Trp Asn - Lys. Assuming that the mutation is a single nucleotide substitution, insertion, or deletion, what is the sequence of the mutant mrna? (1 Point) What mutagen could have been used to induce this mutation? (2 Points) d) A third mutant version of this mrna was identified and the mutant protein was sequenced. The sequence of the mutant protein is Met Pro Trp - Gln. Assuming that the mutation is a single nucleotide substitution, insertion, or deletion, what is the sequence of the mutant mrna? (1 Point) What mutagen could have been used to induce this mutation? (2 Points) 7
7 Problem 3. (10 Points) Each of the following describes a genotype of an E. coli merodiploid strain. Assume all strains are wild type for the CAP gene and for the CAP binding site in lacp. Answer the questions below each genotype. a) laci + laco C lacp + lacz - lacy + AND laci - laco + lacp + lacz + lacy - a) laci - laco C lacp + lacz + lacy - AND laci + laco + lacp + lacz - lacy + a) laci S laco + lacp + lacz - lacy + AND laci - laco + lacp + lacz + lacy - a) laci S laco + lacp + lacz + lacy - AND laci + laco C lacp + lacz - lacy + Will functional β-galactosidase or galactose permease be synthesized by this bacterium on medium contain a 8
8 Problem 3. (10 Points) Each of the following describes a genotype of an E. coli merodiploid strain. Assume all strains are wild type for the CAP gene and for the CAP binding site in lacp. Answer the questions below each genotype. a) laci + laco + lacp + lacz - lacy + AND laci - laco C lacp + lacz + lacy - a) laci + laco + lacp + lacz + lacy - AND laci - laco C lacp + lacz - lacy + a) laci S laco + lacp + lacz - lacy + AND laci - laco C lacp + lacz + lacy - a) laci S laco + lacp + lacz + lacy - AND laci + laco + lacp + lacz - lacy + Will functional β-galactosidase or galactose permease be synthesized by this bacterium on medium contain a 9
9 Problem 4. (10 Points) The figure shows the results of a replica plating experiment using four yeast strains, (A, B, C and D) and three types of medium (YPD, Aminoadipate medium and Minus Lysine medium). Strain D is wild type yeast. UV Irradiated indicates the portion of the plate subjected to UV irradiation immediately after replica plating. All plates were incubated in the dark after irradiation. The completely black-shaded areas indicate growth of all cells plated. The black dots indicate that only some cells that were plated grew into colonies. Each dot is a single yeast colony Use this figure to answer the following questions. a) What genetic difference between strains A, B, C or D is suggested by the results shown on the YPD plate? b) What genetic difference between strains A, B, C or D is suggested by the results shown on the Minus Lysine plate? c) What genetic difference between strains B and D is suggested by the results shown on the No UV portion of the Aminoadipate plate? d) What is demonstrated by the difference in the results for strain D on the No UV portion of the Aminoadipate plate compared to the UV Irradiated portion of the Aminoadipate plate? 10
10 Problem 4. (10 Points) The figure shows the results of a replica plating experiment using four yeast strains, (A, B, C and D) and three types of medium (YPD, Aminoadipate medium and Minus Lysine medium). Strain A is wild type yeast. UV Irradiated indicates the portion of the plate subjected to UV irradiation immediately after replica plating. All plates were incubated in the dark after irradiation. The completely black-shaded areas indicate growth of all cells plated. The black dots indicate that only some cells that were plated grew into colonies. Each dot is a single yeast colony Use this figure to answer the following questions. a) What genetic difference between strains A, B, C or D is suggested by the results shown on the YPD plate? b) What genetic difference between strains A, B, C or D is suggested by the results shown on the Minus Lysine plate? c) What genetic difference between strains C and A is suggested by the results shown on the No UV portion of the Aminoadipate plate? d) What is demonstrated by the difference in the results for strain A on the No UV portion of the Aminoadipate plate compared to the UV Irradiated portion of the Aminoadipate plate? 12
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