Prep Columns. and Bulk Media. and Bulk Media

Transcription

1 Prep Columns and Bulk Media Axia Columns Process Chromatography Bulk Media Columns, Scout and Preparative Sepra Bulk Sorbents Prep Columns and Bulk Media

2 Axia U.S. Patent No. 7, 674, 383 Axia Preparative Chromatography Redefined Axia patented technology is an advanced column packing and hardware design that eliminates media bed collapse as a source of premature failure in chiral and achiral preparative columns. If Axia packed columns do not provide at least an equivalent separation as compared to a competing preparative column of the same particle size, same phase, and dimensions, return the column with comparative data within 45 days for a FULL REFUND. Only applies to. mm ID columns. Axia Hydraulic Piston Compression First ever integrated packing piston in a column allows: The piston compresses the media bed and pressure is never released (no chance of media loss or recompression) The optimum slurry amount and packing pressure are automated for reproducible packing control Uniform packing density and consistent peak shape every time A complete departure from conventional slurry packing methods means: Longer lifetime with patented technology Improved column-to-column reproducibility Recover higher compound purity with analytical-like efficiency View the packing Packing piston locked. No media or pressure escape Media maintains initial optimal packing density and shape Silica media packed under piston pressure PREP COLUMNS & bulk media AXIA Column Specialized computer control for ideal pressure SEM of Inlet Frits After Packing Media Traditional Packed Media Media is largely intact at frit surface after packing Inlet frit shows crushed media particles or fines at frit surface after packing 346 Phenomenex

3 Axia U.S. Patent No. 7, 674, 383 Expect Better Performance. Expect an Excellent Axia Column. Every Time. A completely automated packing system offers feedback control and infinite tuning of packing density to specific media characteristics such as mechanical strength and porosity. An optimum higher bed density can be consistently reproduced column-to-column. This directly translates into consistent efficiency and peak asymmetry measurements and decreases the column variability seen in traditionally packed preparative columns. Consistent Quality. Column-to-Column. Batch-to-Batch 6 R&D Award Recipient Efficiency (N),, 8, Reproducible Column-to-Column Efficiency Average Efficiency (N) with Synergi 4 µm Hydro-RP x. mm.8 % RSD.5 7 % Improved Avg. Efficiency Peak Asymmetry.4.3. Reproducible Column-to-Column Peak Asymmetry Average Peak Asymmetry with Gemini 5 µm C8 5 x. mm 9. % RSD 3 % Improved Avg. Peak Shape 5. Density (%) Density Comparison of Packed Beds 9 % Increase in Packing Density and More Uniformly Packed Bed 6, , Conventional Slurry Packing Hydraulic Piston Compression Packing Conventional Slurry Packing Hydraulic Piston Compression Packing 53 Conventional Slurry Packing Hydraulic Piston Compression Packing Compare Lifetime mau 5 Luna 5 µm C8() 3 App ID 597 vs. Leading Competitive Preparative Column Waters XTerra 5 µm Prep MS C8 OBD mau 3 5 App ID 5933 AXIA PREP COLUMNS & bulk media 3 4 min 3 4 Firsun 8th Run Firsun th Run Six columns tested. Best lifetime chosen for comparison. Conditions for all except where noted: Columns: Luna 5 µm C8() Waters XTerra 5 µm Prep MS C8 OBD Dimension: 5 x. mm (Luna) 5 x 9 mm (XTerra ) Mobile Phase: A:.5 % TFA in Water B:.5 % TFA in Acetonitrile Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min Injection Volume: 5 μl Flow Rate: 3 ml/min (Luna) 4 ml/min (XTerra ) Detection: 54 nm Sample:. Triprolidine.6 mg. Methacycline 6 mg 3. Amitriptyline 5.5 mg Same linear velocity Visit to view the technology in action. Comparative separations may not be representative of all applications. Phenomenex 347

4 PREP COLUMNS & bulk media AXIA Axia U.S. Patent No. 7, 674, 383 Seamless Scalability: Options to Increase Sample Load Option : Increase Column Length Increase sample load without increasing your flow rate by using a longer column. With Axia technology, each preparative column is optimized for: Analytical-like efficiency Long column lifetime High sample load with high-surface area media such as Gemini, Luna, or Synergi As a result, load generally increases as a direct proportion to column length. In this example the sample load tripled by increasing column length. 3 mg on x. mm Column Option : Increase Column ID For maximizing load without increasing the run time, consider scaling up to a larger column ID. Axia packed columns provide the three important benefits you need. Reproducible performance across all column diameters Increased throughput without sacrificing purity High efficiency from analytical to preparative 5 x 4.6 mm 64 µg SF= Plates/Meter 87 Asymmetry.6 3 min 3x load min App ID 86 App ID mg on 5 x. mm Column Columns: Luna 5 μm C8() Gradient: 5 min, 5 % to 95 % Water/Acetonitrile,.5 % TFA Flow Rate:.5 ml/min on 4.6 mm 3 ml/min on. mm 6 ml/min on 3 mm 5 ml/min on 5 mm Detection: 54 nm Sample:. Propranolol. Diphenhydramine 5 x 3 mm.4 mg SF=4 Plates/Meter 8335 Asymmetry.97 Gradient: min on mm length column 5 min on 5 mm length column 5 % to 95 % Acetonitrile,.5 % TFA Flow Rate: 3 ml/min min 3 min App ID 864 App ID x. mm. mg SF= Plates/Meter 8438 Asymmetry.7 5 x 5 mm 7.3 mg SF=8 Plates/Meter 888 Asymmetry. App ID 863 Same Efficiency as Analytical Column App ID min SF=Scaling Factor 3 min 348 Phenomenex

5 Axia U.S. Patent No. 7, 674, 383 Selectivity Options Stationary Phase Selectivity With high surface areas, Phenomenex media Gemini NX-C8 and Gemini (375 m /g), Luna (4 m /g) and Synergi (475 m /g) maximize loading capabilities. Use the selection tree below to select the best media for your targeted purification. Column Selection Tree Achiral Chiral Yes Aromatic? No Yes Basic Compounds? No Yes Aromatic? No Lux Cellulose- Lux Cellulose- Lux Cellulose-3 Lux Cellulose-4 Lux Amylose- Gemini C6-Phenyl (Basic mobile phase ph recommended) Gemini NX-C8 Gemini C8 (Basic mobile phase ph recommended) Gemini, Lux and Luna are registered trademarks of Phenomenex. Synergi is a trademark of Phenomenex. Kinetex Phenyl-Hexyl Synergi Polar-RP Luna Phenyl-Hexyl Yes Polar? No Kinetex XB-C8 or C8 or C8 Synergi Polar-RP Gemini NX-C8 Synergi Hydro-RP Luna C8() or C8() Luna CN Synergi Max-RP Luna NH ph-lc Selectivity In reversed phase chromatography, compounds retain better when neutral. With the advent of ph stable (-) media such as Gemini NX-C8, Gemini C8 and C6-Phenyl, improving retention and resolution of basic compounds at high ph is now possible without compromising column lifetime. Under these conditions you can easily double or triple the loading compared to your current low ph purifications. ph Selectivity Low ph Column: Luna 5 µm C8() Dimensions: 5 x. mm Part No.: B-45-P-AX Mobile Phase: A:.5 % TFA in Water B:.5 % TFA in Acetonitrile Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min Injection Volume: 5 μl Flow Rate: 3 ml/min Detection: 54 nm Sample:. Triprolidine.6 mg. Methacycline 6 mg 3. Amitriptyline 5.5 mg High ph Columns: Gemini µm C8 Dimensions: 5 x. mm Part No.: B-4436-P-AX Mobile Phase: A: 95:5 5 mm Ammonium Bicarbonate, ph. / Acetonitrile B: 5:95 5 mm Ammonium Bicarbonate, ph. / Acetonitrile Gradient: 7 min % A to % B over 7 min, equilibrate for 3 min Injection Volume: 5 μl Flow Rate: 3 ml/min Detection: 54 nm Sample:. Triprolidine.6 mg. Methacycline 6 mg 3. Amitriptyline 5.5 mg Luna 5 μm C8() min Gemini μm C min 3 App ID 5973 App ID 598 Increase Sample Load 3 mg load mg load 6 mg load min Increase ph for Basic Compounds Baseline Resolution for Higher Purity min ph. ph. App ID 777 App ID 776 App ID min Low ph Column: Luna 5 µm C8() Dimensions: 5 x. mm Part No.: B-45-P-AX Mobile Phase: A:.5 % TFA in Water, ph. B: Acetonitrile Gradient: 95:5 to 5:95 (A/B) over 4 minutes Flow Rate: 3 ml/min Detection: 54 nm Sample:. Propranolol. Diphenhydramine High ph Column: Gemini 5 µm C8 Dimensions: 5 x. mm Part No.: B-4435-P-AX Mobile Phase: A: 5 mm Ammonium Bicarbonate, ph. B: Acetonitrile Gradient: 95:5 to 5:95 (A/B) over 5 minutes Flow Rate: 3 ml/min Detection: 54 nm Sample:. Propranolol. Diphenhydramine AXIA PREP COLUMNS & bulk media Phenomenex 349

6 Axia U.S. Patent No. 7, 674, 383 Chiral Media Packed in Axia Technology Resolve 9 % of Your Enantiomers with Lux Chiral Preparative Columns* Five Distinct Polysaccharide Phases to Screen: Lux Cellulose-: Dimethyl Cellulose Chiral Selector Cellulose tris (3, 5-dimethylphenylcarbamate) Lux Cellulose-: Chlorinated Cellulose Carbamate Phase Cellulose tris (3-chloro-4-methylphenylcarbamate) Lux Cellulose-3: Cellulose Ester Chiral Selector Cellulose tris (4-methylbenzoate) Lux Cellulose-4: Chlorinated Cellulose Carbamate Phase Cellulose tris (4-chloro-3-methylphenylcarbamate) Lux Amylose-: Chlorinated Amylose Chiral Selector Amylose tris (5-chloro--methylphenylcarbamate) * based on screening 33 compounds on the five Lux phases Availability in 3 µm and 5 µm packed columns as well as µm bulk media for process scale purification All Lux columns are pressure stable up to 3 bar and ph stable -9 Higher Purity Preparative Separations With award-winning Axia technology, analytical-like efficiency is achieved in a preparative column format. High Efficiency trans-stilbene Oxide from Analytical to Preparative mm ID N = 7,664 a =.9 App ID mm ID N = 77,8 a =. App ID 884 PREP COLUMNS & bulk media AXIA 4 6 Conditions for all separations except where noted: Column: Lux 5 µm Cellulose- Dimensions: ) 5 x 4.6 mm ) 5 x. mm 3) 5 x 5 mm Mobile Phase: Hexane/Isopropanol (9:) Flow Rates: ).5 ml/min ) ml/min 3) 5 ml/min Detection: nm Injections: ) µl ) µl 3) 3 µl min Direct Scale Up of Methocarbamol on Lux Cellulose- x 4.6 mm 5 µg a =.9 min Conditions for all separations except where noted: Column: Lux 5 µm Cellulose- Dimensions: ) x 4.6 mm ) x. mm 3) 5 x. mm Mobile Phase: Methanol/Isopropanol (9:) Flow Rates: ). ml/min ) ml/min 3) ml/min Detection: ) nm ) 54 nm 3) 54 nm Injections: ) 5 µg in µl ) 3 mg in 64 µl 3) 8 mg in 6 µl Increase ID App ID mm ID N = 75,56 a = x. mm 3 mg a = min No Resolution Lost with Increased Sample Load 5 x. mm 8 mg a = min min Same Efficiency as Analytical Column Increase Length.5x Load Increase App ID 778 App ID 885 App ID 778 min 35 Phenomenex

7 Axia U.S. Patent No. 7, 674, 383 Axia: Approved Complete Screening From analytical to Axia packed preparative achiral columns, Luna, Gemini, Synergi, and Lux chiral columns offer complementary selectivities, high efficiency, and pressure stability up to 3 bar (43 psi) for separations. Seamless Scale Up from Laboratory, to Pilot Plant and Production. Increase column ID for higher loading and greater purification. Axia packed. and 3 mm diameter columns provide same purification capability and performance as the 4.6 mm analytical screening columns. Baseline Separation of Enantiomers UV-VIS Lux Cellulose- offers great peak shape at nm O H N O H Terfenadine C(CH 3 ) 3 C(CH ) min Polarimeter App ID 8865 Dimensions: 5 x 4.6 mm Flow Rate:.5 ml/min Detection: nm Load: 3 µg in µl UV-VIS 5x Load Increase Conditions for all columns: Columns: Lux 5 µm Cellulose- Mobile Phase: Methanol with. % DEA/ Carbon Dioxide (5:75) Column Temperature: 35 C Polarimeter: ALP-PDR-Chiral Sample: Terfenadine with ethanol dissolution solvent min Polarimeter Overloading study with increased analytical load showing impurities eluting after major enantiomers only detected at 54 nm x Load Increase App ID 8866 Dimensions: 5 x 4.6 mm Flow Rate:.5 ml/min Detection: 54 nm Load:.5 mg in 5 µl High loading capacity media along with stacking injections allow for increased yields Closer stacked injections can not be used due to the impurities eluting after the major enantiomers AXIA PREP COLUMNS & bulk media App ID 8867 Dimensions: 5 x. mm Flow Rate: 5 ml/min Detection: nm Load: 5 mg in 3.5 ml 7.5 cycles per hr/ 787 mg per hr For more applications, see pp Phenomenex 35

8 Axia U.S. Patent No. 7, 674, 383 First and Only Core-Shell Material for Preparative Purifications Kinetex Preparative HPLC Columns Core-shell performance in a preparative format Easy method scale-up from Kinetex analytical HPLC and UHPLC columns Reduce solvent consumption with faster purifications 5 µm Kinetex Core-Shell Up to % efficiency increase in preparative columns VS. 5 µm Fully Porous The recent addition of the Kinetex 5 µm in the Axia packed format (. mm ID) makes it the first core-shell sorbent commercially available for small-scale preparative applications. Combine this with the added flexibility that the entire Kinetex core-shell line (.3 µm,.7 µm,.6 µm and 5 μm) is fully scalable in retention and selectivity, makes transferring high performance HPLC/UHPLC methods to preparative and applications, simple. Seamless Scalability from HPLC/UHPLC to PREP Analytical method Kinetex.6 μm XB-C8 PREP COLUMNS & bulk media AXIA mau Preparative scale-up and fraction collection Kinetex 5 μm XB-C8 mv min FR FR FR 3 FR min Analytical fraction analysis Kinetex.6 μm XB-C8 Fraction 4 Fraction 3 Fraction Fraction min App ID 454 App ID 45 App ID 453 Column: Kinetex.6 µm XB-C8 Dimensions: 5 x 4.6 mm Part No.: F-4496-E Mobile Phase: A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: Linear 85:5 (A/B) to 5:95 (A/B) over minutes Injection Volume: µl Flow Rate: ml/ min Detection: nm Sample: Crude peptide mix Column: Kinetex 5 µm XB-C8 Dimensions: 5 x. mm Part No.: F-465-P-AX Mobile Phase: A:.% TFA in Water B:.% TFA in Acetonitrile Gradient: Linear 85:5 (A/B) to 5:95 (A/B) over minutes Injection Volume: ml Flow Rate: ml/ min Detection: nm Sample: Crude peptide mix Column: Kinetex.6 µm XB-C8 Dimensions: 5 x 4.6 mm Part No.: F-4496-E Mobile Phase: A:. % TFA in Water B:. % TFA in Acetonitrile Gradient: Linear 85:5 (A/B) to 5:95 (A/B) over minutes Injection Volume: µl Flow Rate: ml/ min Detection: nm Sample: Purified fractions For more information on the power of Kinetex core-shell scalability, request technical note TN-35 at: For more Kinetex information, see p Phenomenex

9 Axia Excellent Loadability and Higher Efficiencies Axia columns packed with Kinetex 5 μm core-shell media provide higher efficiencies and loadability that is as good or better than columns packed with fully porous 5 μm media. Even under very challenging conditions, such as the purification of strong bases using a mobile phase containing formic acid (. %) as the modifier, the Axia-packed Kinetex 5 µm media outperforms a fully porous Waters XBridge Prep column. Compare Loadability Waters XBridge 5 µm C8 Prep OBD 5 x 9 mm Kinetex 5 µm C8 5 x. mm mv min Conditions for both columns: Columns: Kinetex 5 μm C8 XBridge 5 µm C8 Prep OBD Dimensions: 5 x. mm (Kinetex) 5 x 9 mm (XBridge) Mobile Phase: A: Water with.5 % Formic acid B: Acetonitrile with.5 % Formic acid Gradient: Time (min) 8 Compare Efficiencies Waters XBridge 5 µm C8 Prep OBD 5 x 9 mm 5 3 Response - Millivolts 4 N =,553 plates SecurityGuard PREP System (Highly recommended for extending column lifetime) Protect your column and prolong its lifetime with SecurityGuard, the advanced HPLC guard cartridge system. Get full protection with minimal impact on your chromatographic results. Contaminants are retained by an inexpensive, 5 x. or 5 x 3 mm ID disposable cartridge. See p. 54. For Kinetex Core-Shell SecurityGuard PREP cartridges, see p.. % B min Conditions for both columns: Columns: Kinetex 5 µm XB-C8 XBridge 5 µm C8 Prep OBD Dimensions: 5 x. mm (Kinetex) 5 x 9 mm (XBridge) Mobile Phase: Water/ Acetonitrile (5:5) Injection Volume: µl App ID 775 App ID 456 mv Excellent loadability! min Response - Millivolts Flow Rate: 3 ml/min Detection: 54 nm Sample: mg/ml in DMSO. Doxepin (From - 5 mg on-column). Amitriptyline (From - 5 mg on-column) Kinetex 5 µm XB-C8 5 x. mm N = 8,43 plates min Flow Rate: 5 ml/ min Detection: 54 nm Sample:. Uracil. Acetophenone 3. Toluene 4. Naphthalene Ordering Information SecurityGuard PREP System Part No. Description Unit Price AJ-83 SecurityGuard PREP HPLC Guard Cartridge Holder Kit, ea. mm ID, includes column coupler AJ-877 SecurityGuard PREP HPLC Guard Cartridge Holder Kit, 3. mm ID, includes column coupler ea SecurityGuard PREP Over 55% Increase in Efficiency App ID 774 App ID 455 AXIA PREP COLUMNS & bulk media Waters and XBridge are registered trademarks, OBD is a trademark of Waters Corp. Phenomenex is in no way affiliated with Waters Corp. Comparative separations may not be representative of all applications. Phenomenex 353

10 PREP COLUMNS & bulk media AXIA Axia U.S. Patent No. 7, 674, 383 Columns Ordering Information Phases Part No. Price 5 x. mm Gemini 5 µm C8 B-4435-P-AX Gemini µm C8 B-4436-P-AX Gemini 5 µm NX-C8 B-4454-P-AX Gemini µm NX-C8 B-4455-P-AX Kinetex 5 µm XB-C8 B-465-P-AX Kinetex 5 µm C8 B-46-P-AX Kinetex 5 µm C8 B-468-P-AX Kinetex 5 µm Phenyl-Hexyl B-463-P-AX Luna 5 µm C8() B-45-P-AX Luna µm C8() B-453-P-AX Luna 5 µm C8() B-449-P-AX Luna µm C8() B-45-P-AX Luna 5 µm CN B-455-P-AX Luna 5 µm PFP() B-4448-P-AX Luna 5 µm Phenyl-Hexyl B-457-P-AX Luna 5 µm HILIC B-445-P-AX Luna 5 µm Silica() B-474-P-AX Synergi 4 µm Fusion-RP B-444-P-AX Synergi 4 µm Max-RP B-4337-P-AX Synergi 4 µm Hydro-RP B-4375-P-AX Synergi 4 µm Polar-RP B-4336-P-AX 75 x. mm Gemini 5 µm C8 C-4435-P-AX Gemini 5 µm NX-C8 C-4454-P-AX Luna 5 µm C8() C-45-P-AX x. mm Gemini 5 µm C8 D-4435-P-AX Gemini µm C8 D-4436-P-AX Gemini 5 µm C6-Phenyl D-4444-P-AX Gemini 5 µm NX-C8 D-4454-P-AX Gemini µm NX-C8 D-4455-P-AX Jupiter µm C8 D-455-P-AX Jupiter µm C4 D-468-P-AX Jupiter µm Proteo D-4397-P-AX Kinetex 5 µm XB-C8 D-465-P-AX Kinetex 5 µm C8 D-46-P-AX Kinetex 5 µm C8 D-468-P-AX Kinetex 5 µm Phenyl-Hexyl D-463-P-AX Luna 5 µm C8() D-45-P-AX Luna µm C8() D-453-P-AX Luna 5 µm C8() D-449-P-AX Luna 5 µm PFP() D-4448-P-AX Luna 5 µm Phenyl-Hexyl D-457-P-AX Luna 5 µm NH D-4378-P-AX Luna 5 µm HILIC D-445-P-AX Luna 5 µm Silica () D-474-P-AX Lux 5 µm Amylose- D-447-P-AX Lux 5 µm Cellulose- D-4459-P-AX Lux 5 µm Cellulose- D-4457-P-AX Synergi 4 µm Fusion-RP D-444-P-AX Synergi 4 µm Max-RP D-4337-P-AX Synergi µm Max-RP D-435-P-AX Synergi 4 µm Hydro-RP D-4375-P-AX Synergi 4 µm Polar-RP D-4336-P-AX 5 x. mm Gemini 5 µm C8 F-4435-P-AX Gemini µm C8 F-4436-P-AX Gemini 5 µm C6-Phenyl F-4444-P-AX Gemini 5 µm NX-C8 F-4454-P-AX Gemini µm NX-C8 F-4455-P-AX Kinetex 5 µm XB-C8 F-465-P-AX If Axia packed columns do not provide at least an equivalent separation as compared to a competing preparative column of the same particle size, same phase, and dimensions, return the column with comparative data within 45 days for a full refund. Only applies to. mm ID columns. Phases Part No. Price 5 x. mm (cont d) Kinetex 5 µm C8 F-46-P-AX Kinetex 5 µm C8 F-468-P-AX Kinetex 5 µm Phenyl-Hexyl F-463-P-AX Luna 5 µm C8() F-45-P-AX Luna µm C8() F-453-P-AX Luna 5 µm C8() F-449-P-AX Luna µm C8() F-45-P-AX Luna 5 µm CN F-455-P-AX Luna 5 µm NH F-4378-P-AX Luna 5 µm PFP() F-4448-P-AX Luna 5 µm Phenyl-Hexyl F-457-P-AX Luna 5 µm HILIC F-445-P-AX Luna 5 µm Silica() F-474-P-AX Lux 5 µm Amylose- F-447-P-AX Lux 5 µm Cellulose- F-4459-P-AX Lux 5 µm Cellulose- F-4457-P-AX Lux 5 µm Cellulose-3 F-4493-P-AX Lux 5 µm Cellulose-4 F-449-P-AX Synergi 4 µm Max-RP F-4337-P-AX Synergi 4 µm Hydro-RP F-4375-P-AX Synergi 4 µm Polar-RP F-4336-P-AX Synergi 4 µm Fusion-RP F-444-P-AX 5 x. mm Gemini 5 µm C8 G-4435-P-AX Gemini µm C8 G-4436-P-AX Gemini 5 µm C6-Phenyl G-4444-P-AX Gemini 5 µm NX-C8 G-4454-P-AX Gemini µm NX-C8 G-4455-P-AX Jupiter µm C4 G-468-P-AX Jupiter 4 µm Proteo G-4396-P-AX Jupiter µm Proteo G-4397-P-AX Kinetex 5 µm XB-C8 G-465-P-AX Kinetex 5 µm C8 G-46-P-AX Kinetex 5 µm C8 G-468-P-AX Kinetex 5 µm Phenyl-Hexyl G-463-P-AX Luna 5 µm C8() G-45-P-AX Luna µm C8() G-453-P-AX Luna 5 µm C8() G-449-P-AX Luna µm C8() G-45-P-AX Luna 5 µm CN G-455-P-AX Luna 5 µm NH G-4378-P-AX Luna 5 µm PFP() G-4448-P-AX Luna 5 µm Phenyl-Hexyl G-457-P-AX Luna 5 µm Silica() G-474-P-AX Luna µm Silica() G-49-P-AX Lux 5 µm Amylose- G-447-P-AX Lux 5 µm Cellulose- G-4459-P-AX Lux 5 µm Cellulose- G-4457-P-AX Lux 5 µm Cellulose-3 G-4493-P-AX Lux 5 µm Cellulose-4 G-449-P-AX Synergi 4 µm Max-RP G-4337-P-AX Synergi 4 µm Hydro-RP G-4375-P-AX Synergi 4 µm Polar-RP G-4336-P-AX Synergi 4 µm Fusion-RP G-444-P-AX continued Make you Axia columns last longer with SecurityGuard PREP Holders and Cartridges. See p Phenomenex

11 Axia U.S. Patent No. 7, 674, 383 Columns (cont d) If Axia packed columns do not provide at least an equivalent separation as compared to a competing preparative column of the same particle size, same phase, and dimensions, return the column with comparative data within 45 days for a full refund. Only applies to. mm ID columns. Ordering Information (cont d) Phases Part No. Price 5 x 3 mm Gemini 5 µm C8 B-4435-U-AX Gemini µm C8 B-4436-U-AX Gemini 5 µm NX-C8 B-4454-U-AX Gemini µm NX-C8 B-4455-U-AX Kinetex 5 µm C8 B-46-U-AX Luna 5 µm C8() B-45-U-AX Luna µm C8() B-453-U-AX Luna 5 µm C8() B-449-U-AX Luna µm C8() B-45-U-AX Luna 5 µm Phenyl-Hexyl B-457-U-AX Luna 5 µm PFP() B-4448-U-AX Luna 5 µm Silica() B-474-U-AX Synergi 4 µm Max-RP B-4337-U-AX Synergi µm Max-RP B-435-U-AX Synergi 4 µm Hydro-RP B-4375-U-AX Synergi µm Hydro-RP B-4376-U-AX Synergi 4 µm Polar-RP B-4336-U-AX Synergi µm Polar-RP B-435-U-AX 75 x 3 mm Gemini 5 µm C8 C-4435-U-AX Gemini 5 µm C6-Phenyl C-4444-U-AX Gemini 5 µm NX-C8 C-4454-U-AX Luna 5 µm C8() C-45-U-AX Luna µm C8() C-453-U-AX Luna 5 µm C8() C-449-U-AX Luna 5 µm Phenyl-Hexyl C-457-U-AX Synergi 4 µm Max-RP C-4337-U-AX Synergi 4 µm Hydro-RP C-4375-U-AX Synergi 4 µm Polar-RP C-4336-U-AX x 3 mm Gemini 5 µm C8 D-4435-U-AX Gemini µm C8 D-4436-U-AX Gemini 5 µm C6-Phenyl D-4444-U-AX Gemini 5 µm NX-C8 D-4454-U-AX Gemini µm NX-C8 D-4455-U-AX Jupiter µm C8 D-455-U-AX Kinetex 5 µm C8 D-46-U-AX Luna 5 µm C8() D-45-U-AX Luna µm C8() D-453-U-AX Luna 5 µm C8() D-449-U-AX Luna 5 µm CN D-455-U-AX Luna 5 µm PFP() D-4448-U-AX Luna 5 µm Phenyl-Hexyl D-457-U-AX Luna 5 µm Silica() D-474-U-AX Synergi 4 µm Max-RP D-4337-U-AX Synergi µm Max-RP D-435-U-AX Synergi 4 µm Hydro-RP D-4375-U-AX Synergi 4 µm Polar-RP D-4336-U-AX Synergi 4 µm Fusion-RP D-444-U-AX Synergi µm Fusion-RP D-445-U-AX For Axia Reducing Adapter, see p. 367 For Clarity Oligo-RP and Oligo-WAX Axia packed columns, see p. 34 For PREP SecurityGuard Cartridges, see p. 54 For PREP Column In-Line Filter, see p. For information see p. 339 For additional phases and sizes not displayed, please visit the Phenomenex.com website s individual product pages or contact your Phenomenex technical consultant or local distributor. Phenomenex Phases Part No. Price 5 x 3 mm Gemini 5 µm C8 F-4435-U-AX Gemini µm C8 F-4436-U-AX Gemini 5 µm NX-C8 F-4454-U-AX Gemini µm NX-C8 F-4455-U-AX Kinetex 5 µm C8 F-46-U-AX Luna 5 µm C8() F-45-U-AX Luna µm C8() F-453-U-AX Luna 5 µm NH F-4378-U-AX Luna 5 µm PFP() F-4448-U-AX Lux 5 µm Amylose- F-447-U-AX Lux 5 µm Cellulose- F-4457-U-AX Synergi 4 µm Polar-RP F-4336-U-AX 5 x 3 mm Gemini 5 µm C8 G-4435-U-AX Gemini µm C8 G-4436-U-AX Gemini 5 µm NX-C8 G-4454-U-AX Gemini µm NX-C8 G-4455-U-AX Jupiter µm C8 G-455-U-AX Jupiter 4 µm Proteo G-4396-U-AX Jupiter µm Proteo G-4397-U-AX Kinetex 5 µm C8 G-46-U-AX Luna 5 µm C8() G-45-U-AX Luna µm C8() G-453-U-AX Luna 5 µm C8() G-449-U-AX Luna 5 µm CN G-455-U-AX Luna 5 µm PFP() G-4448-U-AX Luna 5 µm HILIC G-445-U-AX Luna 5 µm Silica() G-474-U-AX Lux 5 µm Amylose- G-447-U-AX Lux 5 µm Cellulose- G-4459-U-AX Lux 5 µm Cellulose- G-4457-U-AX Lux 5 µm Cellulose-3 G-4493-U-AX Lux 5 µm Cellulose-4 G-449-U-AX Synergi 4 µm Polar-RP G-4336-U-AX 5 x 5 mm Gemini 5 µm C8 B-4435-V-AX Gemini µm C8 B-4436-V-AX Gemini µm NX-C8 B-4455-V-AX Luna 5 µm C8() B-45-V-AX Luna µm C8() B-453-V-AX x 5 mm Gemini µm C8 D-4436-V-AX Gemini µm NX-C8 D-4455-V-AX Luna µm C8() D-453-V-AX 5 x 5 mm Gemini µm C8 F-4436-V-AX Gemini µm NX-C8 F-4455-V-AX 5 x 5 mm Gemini µm C8 G-4436-V-AX Gemini µm NX-C8 G-4455-V-AX Luna µm C8() G-453-V-AX Luna 5 µm C8() G-473-V-AX Luna µm C8() G-45-V-AX Luna 5 µm C8() G-47-V-AX Lux 5 µm Cellulose- G-4459-V-AX Lux 5 µm Cellulose- G-4457-V-AX Lux 5 µm Cellulose-3 G-4493-V-AX Lux 5 µm Cellulose-4 G-449-V-AX AXIA PREP COLUMNS & bulk media 355

12 Process Chromatography Bulk HPLC Media Grams to Multi-Kilogram, Phenomenex can deliver Over different media available Long lifetime and excellent reproducibility Quick, Direct Scale-up from Analytical Methods Scaling up is easier when using an HPLC media that provides near identical performance across all particle sizes and with increases in column diameter. Any mobile phase conditions developed on a Gemini, Jupiter, Luna or Synergi analytical column can be easily transferred to a μm or 5 μm preparative column with equivalent resolution, selectivity, and proportional mass loading. Lux analytical columns also easily scale to µm preparative columns. Equivalent Performance Across All Particle Sizes Mechanically Strong Media Media free of crushed or cracked silica and silica fines Backpressures that remain stable Consistent particle size distribution so performance is maintained Longer column lifetimes (frits stay unclogged) Withstand Multiple Repacking in Dynamic Axial Compression (DAC) Systems Dynamic Axial Compression (DAC) systems apply high mechanical stress on the packing media. This, along with high flow rates and backpressures can crack or sheer low mechanical strength silica particles, creating silica fines, which will rapidly degrade column efficiency and clog frits. Gemini, Jupiter, Luna, Synergi and Lux media provide exceptional strength over multiple DAC packings without sacrificing performance as well as easily withstanding high mechanical stress. mau 6 4 Luna 5 μm C8() 4 6 min mau 4 Luna μm C8() PREP COLUMNS & bulk media PROCESS CHROMATOGRAPHY mau min 4 6 min Scaling Up with Luna μm C8() min Luna 5 μm C8() 5 x 4.6 mm flow rate =. ml/min.93 mg Berberine ().8 mg Indoprofen () 5 x mm flow rate = 4.7 ml/min min 4.45 mg Berberine () 8.74 mg Indoprofen () min 5 x. mm flow rate = ml/min 7.79 mg Berberine () mg Indoprofen () 356 Phenomenex

13 Process Chromatography Chemically Stable Media Chemical stability at ph levels outside the normal constraints of -7 is a critical factor in today s process environments for several reasons: Allows greater loading capacity Allows optimization of sample solubility ph adjustment to optimize recovery of API Clean-in-Place (CIP) processes by means of a caustic wash Increase Sample Load with ph In reversed phase chromatography, compounds retain better when neutral. With the advent of ph stable (-) process medias such as Gemini C8, improving retention and resolution of your basic compounds at high ph is now possible without compromising column lifetime. Under these conditions you can easily double or triple the loading compared to your current low ph purifications. 5 Luna 3 mg load ph 3 5 Increase ph for basic compounds Gemini 3 mg load ph Improved Resolution! 3 x load 5 Gemini 6 mg load ph 5 5 App ID App ID App ID min min min Low ph Column: Luna 5 µm C8() Dimensions: 5 x. mm Part No.: B-45-P-AX Mobile Phase: A:.5 % TFA in Water, ph. B: Acetonitrile Gradient: 95:5 to 5:95 (A/B) over 4 minutes Flow Rate: 3 ml/min Detection: 54 nm Sample:. Propranolol. Diphenhydramine Extended Media Lifetime even Under Caustic Washes Luna 5 µm C8() Å, ph Stable (.5-) Gemini 5 µm C8 Å, ph Stable (-) ph. High ph Column: Gemini 5 µm C8 Dimensions: 5 x. mm Part No.: B-4435-P-AX Mobile Phase: A: 5 mm Ammonium Bicarbonate, ph. B: Acetonitrile Gradient: 95:5 to 5:95 (A/B) over 5 minutes Flow Rate: 3 ml/min Detection: 54 nm Sample:. Propranolol. Diphenhydramine Efficiency (N) Retention Time (min) ph.5 PROCESS CHROMATOGRAPHY PREP COLUMNS & bulk media Hours of Exposure Test Conditions: Column stability tested under highly basic conditions Continuous flush in mm Na HPO 4 (ph.) in Water/Acetonitrile, 5: Days of Exposure Test Conditions: Flushed with non recycled buffer : Acetonitrile 5 mm Methylpyrrolidine (ph.5), ml/min KEY: KEY: Efficiency, Plates for Toluene Symmetry of Toluene of Toluene (min) Symmetry of Pyridine k of Toluene of Toluene Propranolol Diphenhydramine Nortriptyline Phenomenex 357

14 Process Chromatography Excellent Performance at Low ph Luna 5 µm C8() Å, ph Stable (.5-) Gemini 5 μm C8 Å, ph Stable (-) 4 ph.5 Efficiency (N), Retention Time (min) ph.5 Hours of Exposure Test Conditions: Column stability tested under highly acidic conditions. Continuous flush in. % TFA (ph.5) in Water/Acetonitrile, 5: Days of Exposure Test Conditions: Continuous flush in. % TFA, (ph.5) at room temperature. KEY: KEY: Efficiency, Plates for Toluene Symmetry of Toluene of Toluene (min) Symmetry of Pyridine k of Toluene Naproxen Ethylparaben Tolune Diflunisal PREP COLUMNS & bulk media PROCESS CHROMATOGRAPHY Controlled Manufacturing Process We engineer and manufacture all of our media with your needs as a guideline. Our state-of-the-art facility gives us the capability to provide some of the most consistent media available on the market. With very high loadability, excellent mechanical strength, extended chemical stability, and batch-to-batch reproducibility, it is no wonder why more and more people turn to Phenomenex media every day. Batch-to-Batch Reproducibility Gemini µm C8 Specification Ranges A Certificate of Analysis showing the test results for the batch of material is provided with each Gemini media order. This data ensures batch-to-batch reproducibility Gemini µm C8 Batch Number Metal Sensitivity Silanol Activity Methylene Selectivity Metal Sensitivity Silanol Activity Methylene Selectivity Batch-to-Batch Reproducibility Metal Test and Inertness With over 5 years of proven reproducibility, you can be confident in your choice to develop methods on Luna. The following graph shows consistency in both inertness and hydrophobicity across 4 batches of Luna µm C8() Luna µm C8() Batch Number Hydrogen Bonding Metal Test Exceptional Chemical Stability for Low Leachates The dense bonded phase density of Luna and Synergi provide revolutionary ph.5 stability, with Gemini offering an extended ph range of.-.. The wide ph range of these media provides flexibility in method development allowing for improvements in resolution and greater mass loading of basic compounds (pka > 9) at high ph Hydrophobic Index Inertness Hydrogen Bonding and Hydrophobic Index Please see Sorbent Characteristics chart pp for exact ph limits of each phase. 358 Phenomenex

15 Process Chromatography Prep LC Columns and Bulk HPLC Media Maintain or increase yield with less media Dramatically reduce cost of Prep/Process-LC purifications Withstand multiple axial compression packings with high mechanical strength media Choose the Correct Media for your Application Bonded Phase Sorbent Pore Size (Å) Surface Area (m /g) ph Stability Achiral Media Si (Silica) Luna Silica() μm (bulk) -PREP (bulk) 5 μm (bulk) Particle Size (μm) ("bulk" indicates bulk media available) Density Applications.45 Small Organic Molecules, Steroids, Nutraceuticals, Fat Soluble Vitamins, Tocopherols Luna Silica(3) PREP (bulk).47 Small Organic Molecules, Steroids, Nutraceuticals, Fat Soluble Vitamins, Tocopherols C8 Luna C8() μm (bulk) -PREP (bulk) 5 μm (bulk).58 Pharmaceuticals, Peptides, Nutraceuticals, Agrochemical, Vitamins, Basic Compounds, General Reversed Phase Applications Luna C8(3) PREP (bulk).6 Pharmaceuticals, Peptides, Nutraceuticals, Agrochemical, Vitamins, Basic Compounds, General Reversed Phase Applications Synergi Hydro-RP μm (bulk).55 Very Polar Compounds, Pharmaceuticals, Vitamins, Antibiotics C8 with Polar Endcapping Jupiter 3 C μm (bulk), 5 μm (bulk).44 Hydrophilic Proteins, Oligonucleotides (>3 mer) Gemini C µm (bulk).58 Basic Compounds, increased retention & loadability at high ph, low and high ph applications C Synergi Max-RP μm (bulk).55 Pharmaceuticals, Nutraceuticals, Agrochemical, Vitamins, Amino Acids, Basic Compounds, General Reversed Phase Applications Jupiter Proteo μm (bulk).55 Peptides, Oligonucleotides (<3 mer), sirna C8 Luna C8() μm (bulk) -PREP (bulk) 5 μm (bulk).56 Pharmaceuticals, Peptides, Estrogens, Basic Compounds, General Reversed Phase Applications C4 Luna C4() PREP (bulk).54 Hydrophobic Compounds, Peptides, Small Proteins Jupiter 3 C μm (bulk),.38 Hydrophobic Proteins 5 μm (bulk) Phenyl Luna Phenyl-Hexyl μm (bulk) -PREP (bulk) 5 μm (bulk) Synergi Polar-RP (Ether-Linked Phenyl).58 Polar and Aromatic Compounds, Peptides, Antibiotics, Lipids, Phenols, Sweeteners μm (bulk).55 Polar and Aromatic Compounds, Hydrophilic Peptides, Antibiotics, Phenols, Sweeteners CN (Cyano) Luna CN μm (bulk).55 Polar Compounds, Pharmaceuticals, Hydrophilic Peptides, Esters, Steroids, Phthalates, Compounds with COOH, CO, NH, NHR or NR groups NH (Amino) Luna NH 4.5 μm (bulk).57 Sugars, Sugar Alcohols, Anionic Compounds, Steroids, Vitamins, Nucleosides, Oligonucleotides SCX (Strong Cation Exchange) Chiral Media cellulose tris(3,5- dimethylphenyl carbamate) cellulose tris(3- chloro-4-methyl phenylcarbamate) cellulose tris(4- methylbenzoate) cellulose tris(4- chloro-3-methyl phenylcarbamate) Maintain or Increase Yield with Less Media Higher silica surface area equals greater mass loading. With 4 m /g surface area, Luna has one of the highest surface areas among popular Prep LC media. Even greater mass loading is possible with the 475 m /g surface area of Synergi 8 Å media. Both Synergi and Luna are unique in that they offer high mass loading with low-density, rugged silica; requiring less media to pack a given volume. Thus while less media is needed to pack a given dimension compared to other common prep sorbents, mass loading remains high with peak resolution and purity maintained. Especially for early eluting target compounds, Luna has been shown to provide greater mass loading compared to some common prep media. This allows for increased loading on less media, and more yield per run. Luna SCX 4 7. μm (bulk).54 Amine and polyamine containing compounds, Nucleotides, Peptides Lux Cellulose- 9, μm.6 Enhanced enantioselectivity for aromatic, conjugated and other chiral compounds Lux Cellulose- 9, μm.6 Enhanced enantioselectivity for aromatic, conjugated and other chiral compounds Lux Cellulose-3 9 μm.6 Enhanced enantioselectivity for aromatic, conjugated and other chiral compounds Lux Cellulose-4 9 μm.6 Enhanced enantioselectivity for aromatic, conjugated and other chiral compounds PROCESS CHROMATOGRAPHY PREP COLUMNS & bulk media Phenomenex 359

16 PREP COLUMNS & bulk media PROCESS CHROMATOGRAPHY Process Chromatography Scout and Preparative Columns Achiral Columns Ordering Information Luna μm Semi-Prep and Axia Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x 5 x 5 5 x. 5 x 3 5 x 5 4 x 3.* x 5 x.** 5 x 3 /pk /3pk /ea /ea Silica() G-49-E G-49-N G-49-P-AX G-49-U-AX G-49 V-AX AJ-4348 AJ-73 AJ-79 AJ-83 C8() G-45-E G-45-N G-45-P-AX G-45-U-AX G-45-V-AX AJ-49 AJ-7 AJ-784 AJ-83 C8() G-453-E G-453-N G-453-AK G-453-P-AX G-453-U-AX G-453-V-AX AJ-487 AJ-7 AJ-7839 AJ-83 CN G-43-E G-43-N G-43-P-AX AJ-435 AJ-733 AJ-8 AJ-83 Phenyl-Hexyl G-485-E G-485-N G-485-P-AX G-485-U-AX AJ-435 AJ-734 AJ-784 AJ-833 for ID:3.-8. mm 9-6 mm 8-9 mm 3-49 mm Luna μm-prep Semi-Prep and Axia Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x 5 x. 5 x 3 4 x 3.* x. 5 x.** 5 x 3 /pk /3pk /ea /ea PREP Silica(3) G-467-E G-467-N AJ-4348 AJ-73 PREP C4() G-446-E G-446-N PREP C8() G-433-E G-433-N G-433-P-AX AJ-49 AJ-7 AJ-784 AJ-83 PREP C8(3) G-466-E G-466-N AJ-487 AJ-7 PREP C8() G-434-E G-434-N G-434-P-AX G-434-U-AX AJ-487 AJ-7 AJ-7839 AJ-83 for ID: mm 9-6 mm 8-9 mm 3-49 mm Luna 5 μm Axia Preparative and Pilot Scale Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x. 5 x 3 5 x 5 4 x 3.* 5 x.** 5 x 3 /pk /ea /ea Silica() G-47-E G-47-P-AX G-47-U-AX AJ-4348 AJ-79 AJ-83 C8() G-47-E G-47-P-AX G-47-V-AX AJ-49 AJ-784 AJ-83 C8() G-473-E G-473-P-AX G-473-U-AX G-473-V-AX AJ-487 AJ-7839 AJ-83 for ID: mm 8-9 mm 3-49 mm Gemini μm Semi-Prep and Axia Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x 5 x. 5 x 3 5 x 5 4 x 3.* x 5 x.** 5 x 3 /pk /3pk /ea /ea C8 G-4436-E G-4436-N G-4436-P-AX G-4436-U-AX G-4436-V-AX AJ-7597 AJ-7598 AJ-7846 AJ-838 for ID: mm 9-6 mm 8-9 mm 3-49 mm Jupiter μm Semi-Prep and Axia Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x 5 x. 5 x 3 4 x 3.* x 5 x.** 5 x 3 /pk /3pk /ea /ea C4 3 Å G-468-E G-468-N G-468-P-AX G-468-U-AX AJ-433 AJ-75 AJ-73 AJ-834 C8 3 Å G-455-E G-455-N AJ-43 AJ-74 AJ-73 AJ-833 Proteo 9 Å G-4397-E G-4397-N G-4397-P-AX G-4397-U-AX AJ-674 AJ-775 AJ-784 AJ-834 for ID: mm 9-6 mm 8-9 mm 3-49 mm Synergi μm Analytical, Semi-Prep, Axia Preparative and Pilot Scale Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x 5 x 5 5 x. 5 x 3 4 x 3.* x 5 x. ** 5 x 3 /pk /3pk /ea /ea Max-RP G-435-E G-435-N G-435-P-AX G-435-U-AX AJ-674 AJ-775 AJ-784 AJ-834 Polar-RP G-435-E G-435-N G-435-P-AX G-435-U-AX AJ-676 AJ-776 AJ-7845 AJ-837 Hydro-RP G-4376-E G-4376-N G-4376-AK G-4376-P-AX G-4376-U-AX AJ-75 AJ-75 AJ-7843 AJ-835 Fusion-RP G-445-E G-445-N G-445-P-AX G-445-U-AX AJ-7557 AJ-7558 AJ-7844 AJ-836 for ID: mm 9-6 mm 8-9 mm 3-49 mm Chiral Columns Ordering Information Lux Analytical and Axia Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x x 5 x. 5 x 5 4 x 3.* x 5 x.** 5 x 3 μm /pk /3pk /ea /ea Lux Cellulose- G-45-E G-45-N AJ-843 AJ-844 AJ-845 AJ-846 Lux Cellulose- G-45-E G-45-N AJ-8366 AJ-8399 AJ-84 AJ-84 μm /pk /3pk /ea /ea Lux Cellulose- F-4473-E G-4473-E G-4573-N AJ-843 AJ-844 AJ-845 AJ-846 Lux Cellulose- F-4464-E G-4464-E G-4564-N AJ-8366 AJ-8399 AJ-84 AJ-84 Lux Cellulose-3 F-454-E G-454-E G-454-N AJ-86 AJ-863 AJ-864 AJ-865 Lux Cellulose-4 F-453-E G-453-E G-453-N AJ-867 AJ-868 AJ-869 AJ-863 for ID: mm 9-6 mm 8-9 mm 3-49 mm For additional sizes not listed, please visit the individual *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-48 SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ-7 product pages or contact your Phenomenex technical consultant **PREP SecurityGuard Cartridges require holder, Part No.: AJ-83 or local distributor. PREP SecurityGuard Cartridges require holder, Part No.: AJ Phenomenex

17 Process Chromatography Bulk HPLC Media Achiral Media Ordering Information Clarity Phases g kg 5 kg kg μm Clarity Oligo-RP 4G K L M-4445 Clarity Oligo-WAX 4G-445 4K-445 4L-445 4M-445 Gemini ( Å) Phases g kg 5 kg kg μm C8 4G K L M-4436 Luna ( Å) Phases g kg 5 kg kg 5 kg kg μm Silica() 4G-49 4K-49 4L-49 4M-49 4N-49 4P-49 C8() 4G-45 4K-45 4L-45 4M-45 4N-45 4P-45 C8() 4G-453 4K-453 4L-453 4M-453 4N-453 4P-453 CN 4G-43 4K-43 4L-43 4M-43 4N-43 4P-43 NH 4G K L M N P-4379 SCX 4G-44 4K-44 4L-44 4M-44 4N-44 4P-44 Phenyl-Hexyl 4G-485 4K-485 4L-485 4M-485 4N-485 4P-485 μm-prep Silica(3) 4G-467 4K-467 4L-467 4M-467 4N-467 4P-467 Silica() 4G-43 4K-43 4L-43 4M-43 4N-43 4P-43 C4() 4G-446 4K-446 4L-446 4M-446 4N-446 4P-446 C8() 4G-433 4K-433 4L-433 4M-433 4N-433 4P-433 C8(3) 4G-466 4K-466 4L-466 4M-466 4N-466 4P-466 C8() 4G-434 4K-434 4L-434 4M-434 4N-434 4P-434 Phenyl-Hexyl 4G-435 4K-435 4L-435 4M-435 4N-435 4P μm Silica() 4G-47 4K-47 4L-47 4M-47 4N-47 4P-47 C8() 4G-47 4K-47 4L-47 4M-47 4N-47 4P-47 C8() 4G-473 4K-473 4L-473 4M-473 4N-473 4P-473 Phenyl-Hexyl 4G-486 4K-486 4L-486 4M-486 4N-486 4P-486 Jupiter (3 Å and 9 Å) Phases g kg 5 kg kg 5 kg kg μm 3 Å C4 4G-468 4K-468 4L-468 4M-468 4N-468 4P Å C8 4G-455 4K-455 4L-455 4M-455 4N-455 4P-455 Proteo 9 Å 4G K L M N P μm 3 Å C4 4G-469 4K-469 4L-469 4M-469 4N-469 4P Å C8 4G-457 4K-457 4L-457 4M-457 4N-457 4P-457 Synergi (8 Å) Phases g kg 5 kg kg 5 kg kg μm Max-RP 4G-435 4K-435 4L-435 4M-435 4N-435 4P-435 Hydro-RP 4G K L M N P-4376 Polar-RP 4G-435 4K-435 4L-435 4M-435 4N-435 4P-435 Fusion-RP 4G-445 4K-445 4L-445 4M-445 4N-445 4P-445 Chiral Media Ordering Information Lux ( Å) Phases g g kg μm Cellulose- 4D-45 4G-45 4K-45 Cellulose- 4D-45 4G-45 4K-45 μm Cellulose- 4D G K-4473 Cellulose- 4D G K-4464 Cellulose-3 4D-454 4G-454 4K-454 Cellulose-4 4D-453 4G-453 4K-453 Contact your Phenomenex technical consultant or local distributor for additional bulk packings and quantities not listed. For additional brand information, please visit the individual product pages or contact your Phenomenex technical consultant or local distributor For Sepra bulk sorbents, see p. 36 PROCESS CHROMATOGRAPHY PREP COLUMNS & bulk media Phenomenex 36

18 Process Chromatography Sepra Bulk Sorbents Provides reproducible recoveries from capture to purification Removes contaminants and eliminates matrix effects Offers controlled selectivity for target analytes Results in high-throughput sample purification If Sepra Bulk products do not perform as well or better than your current SPE product of similar phase, mass and size, send in your comparative data within 45 days and keep the Sepra Bulk for FREE. Phenomenex offers a wide mix of bulk media including an array of large particle media for today s chemists who need effective capture and concentrating resins. Sepra media offers purification of proteins, peptides, nucleic acids, antibodies, tryptic digests, nucleotides, viruses, and small molecular weight pharmaceuticals in a low pressure environment. It is an excellent economical alternative to high pressure RPC while still offering high resolution and loading capacity. SEPRA BULK SORBENTS PREP COLUMNS & bulk media PROCESS CHROMATOGRAPHY Capture and Concentrate Resins Ordering Information Particle Surface Carbon Size Pore Size Area Load Sepra Bulk Sorbents Media Base Material Brand Phase (µm) (Å) (m /g) (%) ph Stability Phase g Price kg Price Silica Sepra C8-E C8-E 4G K-4348 C8-T C8-T 4G-445 4K-445 C C8 4G-446 4K-446 Phenyl Phenyl 4G-447 4K-447 CN CN 4G-449 4K-449 NH NH 4G-448 4K-448 Florisil 7 (6/ mesh) Florisil 4G-44 4K-44 SCX SCX 4G-443 4K-443 SAX SAX 4G-444 4K-444 WCX WCX 4G-SO7 4K-SO7 Silica Silica 4G-44 4K-44 EPH 7 Proprietary -7.5 EPH 4G-458 4K-458 Small Pore Polymer Sepra ZT ZT ZT 4G-446 4K-446 ZT-SCX ZT-SCX 4G K-4466 ZT-WCX ZT-WCX 4G K-4478 ZT-SAX ZT-SAX 4G K-4485 ZT-WAX ZT-WAX 4G-4463 Large Pore Polymer Sepra ZTL ZTL ZTL 4G-447 4K-447 ZTL-SCX ZTL-SCX 4G K-4467 ZTL-WCX ZTL-WCX ZTL-SAX ZTL-SAX ZTL-WAX ZTL-WAX 4G K-4494 Styrenedivinylbenzene Sepra Polymer SDB-L SDB-L SDB-L 4G-44 4K-44 Interested in MSPD for your analysis? Please contact us for technique and accessory information. Florisil is a registered trademark of U.S. Silica Co. 36 Phenomenex