MICROBIOLOGICAL PROFILE

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1 Evans Vanodine International plc G L O B A L H Y G I E N E S O L U T I O N S SAFE ZONE PLUS MICROBIOLOGICAL PROFILE

2 2 INTRODUCTION SAFE ZONE PLUS is an unperfumed liquid virucidal disinfectant. SAFE ZONE PLUS is available as a ready-to-use solution. SAFE ZONE PLUS has been tested using European Standard methods to meet specific classification/regulatory demands. CONTENTS PAGE BACTERICIDAL ACTIVITY 3 Enterococcus hirae Escherichia coli Leptospira interrogans Pseudomonas aeruginosa Staphylococcus aureus Bacterial Spores Bacillus subtilis Clostridium difficile FUNGICIDAL ACTIVITY 3 Aspergillus brasiliensis Candida albicans VIRUCIDAL ACTIVITY 4 Avian Influenza Virus (H3N8/HN1) Adenovirus Bovine Coronavirus ECBO Virus Influenza A (H1N1/H7N9) Norovirus Poliovirus Polyomavirus SV 40 Rotavirus Vacciniavirus

3 3 Activity against bacteria BACTERIA DISEASE / INFECTION Suspension Test methods Surface Test methods time (mins) time (mins) C D C D Enterococcus hirae Urinary tract infections EN EN EN Escherichia coli Food poisoning EN Pseudomonas aeruginosa Opportunistic pathogen, wound, burn infections EN 1276 EN EN EN Leptospira interrogans Methicillin resistant Staphylococcus aureus Leptospirosis (Weils disease) Skin, bone and wound infections EN 1276 EN 1276 Staphylococcus aureus Skin, bone and wound infections EN 1276 EN EN EN Bacillus subtilis spores Opportunistic pathogen EN Clostridium difficile spores Diarrhoea, abdominal cramps EN Activity against fungi FUNGI DISEASE / INFECTION Suspension Test methods Surface Test methods Aspergillus brasiliensis Ear infections EN 160 EN time (mins) time (mins) C D C D 1 EN Candida albicans Oral and genital infections, candidiasis EN 160 EN EN EN C = CLEAN D = DIRTY

4 4 Activity against viruses VIRUS DISEASE / INFECTION time (mins) Test method Avian Influenza Virus (H3N8/HN1) Avian influenza that can be transmitted to man C D Suspension 10 EN Adenovirus Respiratory infections EN Bovine Coronavirus Calf enteritis 1 EN ECBO Virus Influenza A H1N1 Reproductive, respiratory, or enteric disease in cattle Common cause of human influenza 30 RKI and DVV* 1 EN Influenza A H7N9 Influenza EN Norovirus Diarrhoea and vomiting, gastroenteritis 1 EN Poliovirus Polio EN14476 Polyomavirus SV 40 Rotavirus Vacciniavirus Kidney, respiratory and brain infections and potentially oncogenic (tumour causing) Severe vomiting and diarrhoea in infants and young children Infection following vaccination 30 RKI and DVV* 1 RKI and DVV* RKI and DVV* *Robert Koch Institute is the public health institute in Germany and DVV is the German Association for the Control of Virus Diseases.

5 TEST METHOD REFERENCES EUROPEAN STANDARD: EN 1276 bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas Bactericidal Criteria: minute contact time, 20 C, hard water, dirty and clean conditions. log reduction % reduction. EUROPEAN STANDARD:160 fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas. Fungicidal criteria: 1 minute contact time, 20 C, hard water, low level soiling. EUROPEAN STANDARD:13697 Chemical disinfectants and antiseptics Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas. Without mechanical action. Bactericidal Criteria: minute contact time, 20 C, hard water, dirty conditions. log reduction % reduction. Fungicidal criteria: 1 minute contact time, 20 C, hard water, low level soiling. EUROPEAN STANDARD:13704 Chemical disinfectants Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas. Sporicidal Criteria: 60 minute contact time, 20 C, hard water, low level soiling.

6 6 TEST METHOD REFERENCES EUROPEAN STANDARD:13727 bactericidal activity of chemical disinfectants and antiseptics used in the medical area Bactericidal Criteria: minute contact time, 20 C, hard water, dirty conditions. log reduction % reduction. EUROPEAN STANDARD:13624 fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the medical area. Fungicidal criteria: 1 minute contact time, 20 C, hard water, low level soiling. EUROPEAN STANDARD:1461 Chemical disinfectants and antiseptics Quantitative carrier test for evaluation of bactericidal activity for instruments used in the medical area. Bactericidal criteria: 1 minute contact time, 20 C, low level soiling. log reduction % reduction. EUROPEAN STANDARD:14476 Chemical disinfectants and antiseptics Virucidal quantitative suspension test for chemical disinfectants and antiseptics used in the medical area. Virucidal criteria: minute contact time, 20 C, organic soiling.

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