InertSustain Phenylhexyl. A New Second Choice Column to Change Chromatographic Behavior

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Raymond Hines
5 years ago
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1 InertSustain Phenylhexyl A ew Second Choice Column to Change Chromatographic Behavior

Providing Alternative Selectivity to C8 Phases InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6- carbon) linker and is densely bonded

2 Providing Alternative Selectivity to C8 Phases InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6- carbon) linker and is densely bonded to our newly developed ES silica gel delivering complementary selectivity to straight alkyl-chain columns, but with industry leading reproducibility, inertness and low back pressure. Physical Properties Silica : ES (Evolved Surface) Silica Gel Particle Size : μm, 5 μm Surface Area : 50 m /g Pore Size : 00 Å (0 nm) Pore Volume : 0.85 ml/g Bonded Phase : Phenylhexyl Groups End-capping : Complete Carbon Loading : 9.0 % USP Code : L ph Range :.0 to 0.0 Benefits Different selectivity to C8 phases Strong Retentivity than other Phenyl phases Highly inert packing material results in less tailing of peaks for virtually any type of analytes Deliver extreme sensitivity analysis in LC-MS and LC-MS/MS methods Extreme resistance to low and high ph mobile phases Excellent stability to 00 % aqueous mobile phases Compatible with The European Pharmacopeia (Ph. Eur.) methods Endlessly reproducible from column-to-column and batch-to-batch

3 Different Selectivity to C8 Phases The following selectivity test was used to understand the difference in selectivity between a conventional C8 phase, Phenylhexyl and Phenyl phases. Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH OH B) H O A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 54 nm Sample : 4 5, 6 7 InertSustain C8. Uracil. Caffeine. Phenol 4. n-butylbenzene 5. o-terphenyl 6. n-amylbenzene 7. Triphenylene InertSustain Phenylhexyl Bonded Phase: Phenylhexyl Groups (C6-Ph) InertSustain Phenyl Bonded Phase: Phenyl Groups (C0-Ph) Sample o., Uracil cannot be retained in a reversed phase mode when using the mobile phase condition described on the right. Therefore, Uracil was used to determine the t0 position. t0: Void Volume Sample o.,, Caffeine and Phenol are used to confirm the amount of residual silanol on the surface of the silica gel. Caffeine elutes later against Phenol when there is a lot of residual silanol on the surface of the silica gel. Sample o.4, n-butylbenzene and Sample o.6, n-amylbenzene were used to determine the hydrophobic property of the column. n-amylbenzene elutes later against n-butylbenzene when the hydrophobicity of the column is high. Stereoselectivity is indicated by Sample o.5, o-terphenyl and Sample o.7, Triphenylene. o-terphenyl has a twisted tertiary structure and Triphenylene has a planar structure. Triphenylene elutes later against o-terphenyl when the stereoselectivity of the column is high.

4 Comparison of Selectivity Between a C8 and Phenylhexyl Phase Figure compares the selectivity provided by a conventional C8 and Phenylhexyl phase. InertSustain Phenylhexyl show stronger retention of compounds containing nitro groups. Figure compares the separation of analgesic drugs on a conventional C8 and Phenylhexyl phase. Indomethacin was strongly retained on InertSustain Phenylhexyl due to the presence of strong pi-pi interaction resulting in change in elution order with better separation InertSustain C8 Figure. Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH OH B) H O A/B = 0/70, v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 0 nm Sample :.,,5-Trinitrobenzene.,-Dinitrobenzene. itrobenzene O O O O O.,,5-Trinitrobenzene.,-Dinitrobenzene O InertSustain Phenylhexyl. itrobenzene Analysis of Analgesic Drugs InertSustain C8 Figure. Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH C B) 5 mm KH PO 4 (ph.0, H PO 4 ) A/B = 45/55, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 0 nm Sample :. Indomethacin. Ibuprofen O Cl H C O CH OH H C CH H CH O OH InertSustain Phenylhexyl O. Indomethacin. Ibuprofen

5 Strong Retentivity than other Phenyl Phases The retentivity of InertSustain Phenylhexyl is stronger comparing with commercially available Phenyl columns. Sample o.5, n-amylbenzene was used to determine the hydrophobic property of columns , InertSustain Phenylhexyl 6 6 7, Column Size : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH OH B) H O A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 54 nm Sample :. Uracil. Caffeine. Phenol 4. n-butylbenzene 5. n-amylbenzene 6 6. Triphenylene 7. o-terphenyl XBridge Phenyl Inertsil Ph, , , Luna Phenyl-Hexyl InertSustain Phenyl Zorbax Phenyl-Hexyl Inertsil Ph-

Benefits of Highly Inert Packing Material InertSustain Phenylhexyl also maintains the same extreme inertness, wide ph range and provide separations with symmetric peaks.

6 Benefits of Highly Inert Packing Material InertSustain Phenylhexyl also maintains the same extreme inertness, wide ph range and provide separations with symmetric peaks. Poorly end-capped columns often show tailing of peaks due to the presence of silanols resulting in longer analysis time. Analysis of Antidepressants 4 Eluent Flow Rate Col. Temp. :40 Detection :UV 54 nm Sample :A) CH C B) 5 mm KH PO 4 (ph 7.0, K HPO 4 ) A/B = 50/50, v/v :.0 ml/min :. Imipramine hydrochloride. Amitriptyline hydrochloride. Clomipramine hydrochloride 4. Mianserin hydrochloride 0 0 InertSustain Phenylhexyl. Imipramine hydrochloride. Amitriptyline hydrochloride 4 H C Cl H 0 0 Luna Phenyl-Hexyl. Clomipramine hydrochloride 4. Mianserin hydrochloride

Extreme Sensitivity Analysis in LC-MS and LC-MS/MS Methods The usage of highly inert packing material creates another benefit for LC-MS and LC-MS/MS applications.

7 Extreme Sensitivity Analysis in LC-MS and LC-MS/MS Methods The usage of highly inert packing material creates another benefit for LC-MS and LC-MS/MS applications. As shown below, InertSustain Phenylhexyl deliver excellent peak shape and high sensitivity even with strong basic analyte. H CO H Dextromethorphan CH System Column Eluent : GL QTRAP : μm, 50 x. mm I.D. : A) CH C B) 0 mm CH COOH 4 in H O A/B = 0/70 (gradient mixer ), v/v :0 μl/min Flow rate Col. Temp. :40 Detection :SIM (ESI, Positive ) CUR IS TEM GS GS ihe O Injection Vol. Sample : μl :Dextromethorphan (50 ng/ml).0e6.0e6.0e6 Max..e6 cps..0e6 Max..7e6 cps..0e6.0e InertSustain Phenylhexyl Other Brands Phenyl Column

8 Wide ph Compatibility with Long Column Lifetime As shown in the experiment below, InertSustain Phenylhexyl maintained high efficiency for 0 hours. % Initial Purging Column :5 μm, mm I.D. Eluent :A) 50 mm Triethylamine (ph 9.5) B)CH OH A/B = 70/0, v/v Flow Rate :.0 ml/min Col. Temp. :50 Eluent :CH C/H O = 65/5, v/v Flow Rate :.0 ml/min Col. Temp. :40 Detection :UV 54 nm Sample :aphthalene Hours of Purging (h) Excellent Stability to 00 % Aqueous Mobile Phases When analyzing hydrophilic compounds under water rich mobile phase condition, once the pump is stopped, the hydrophobic bonded group pushes the aqueous mobile phase out off the pore in an irreversible fashion, in what has become known as the dewetting phenomenon. As shown in the following test, InertSustain Phenylhexyl demonstrates excellent stability to dewetting guaranteeing highly stable, reliable and reproducible chromatograms. 4 First Injection Dewetting Test Column : 5 μm, 50 x 4.6 mm I.D. Eluent : 00 % H O Flow rate :.0 ml/min Col. Temp. : 40 Detection : UV 54 nm Sample :.Cytosine.Uracil.Guanine 4.Thymine 5.Adenine InertSustain Phenylhexyl Second Injection Variation: % Testing Procedure ) 00 % water is introduced into column over 60 minutes. ) Conduct analysis. ) Stop flow for 5 minutes. 4) 00 % water is introduced again into column over 0 minutes. 5) Stop flow for 5 minutes again. 6) Conduct analysis.

9 Compatible with The European Pharmacopeia (Ph. Eur.) Methods Analysis of Indomethacin H C O O CH OH O Indomethacin Cl Column : μm, 50 x 4.6 mm I.D. Eluent : A) 0 g/l CH COOH in H O B) CH C A/B = 70/0 min - 70/0 9 min - 50/50 min 50/50-0 min - 70/0 9 min - 0/70 6 min - 0/70, v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 54 nm Injection Vol. : μl Criteria : Indomethacin to be eluted approx. 8 min 0 0 InertSustain Phenylhexyl Access to the latest pharmaceutical, life science, environmental and food applications at

10 Comparison of Performance By conducting the tests below, we can evaluate the degree of inertness of the column. To strictly evaluate all columns, the below mentioned samples were used, which are all known as strong adsorptive compounds. Strong Basic Compound Test Dextromethorphan and Berberine Chloride are strong basic compounds. Severe tailing can be confirmed when the packing material contains residual silanol groups. H CO Column : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH C B) 5 mm K HPO 4 (ph 7.0, KH PO 4 ) A/B = 40/60,v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 0 nm Injection Vol : μl Sample : : Dextromethorphan H CH :Dextromethorphan Strong Acidic Compound Test Brilliant Blue FCF has three sulfonic groups in its chemical structure, severe tailing will occur when the surface of the packing material is slightly basic. - O S SO - SO - Column : 5 μm, 50 x 4.6 mm I.D. Eluent : A) CH C B) 0. % H PO 4 A/B = 5/75,v/v Flow Rate :.0 ml/min Col. Temp. : 40 Detection : UV 54 nm Injection Vol : 5 μl Sample : :Brilliant Blue FCF :Phenol :Salicylic acid + :Brilliant Blue FCF

11 Comparison of Performance Strong Basic Compound Test Strong Acidic Compound Test InertSustain Phenylhexyl : Dextromethorphan : Brilliant Blue FCF : Phenol : Salicylic acid Luna Phenyl-Hexyl. o peak XBridge Phenyl. o peak Zorbax Phenyl-Hexyl. o peak

12 Analytical Columns InertSustain Phenylhexyl HP µm.x0mm InertSustain Phenylhexyl HP µm.x50mm InertSustain Phenylhexyl HP µm.x75mm InertSustain Phenylhexyl HP µm.x00mm InertSustain Phenylhexyl HP µm.x50mm InertSustain Phenylhexyl HP µm.x50mm InertSustain Phenylhexyl HP µm.0x0mm InertSustain Phenylhexyl HP µm.0x50mm InertSustain Phenylhexyl HP µm.0x75mm InertSustain Phenylhexyl HP µm.0x00mm InertSustain Phenylhexyl HP µm.0x50mm InertSustain Phenylhexyl HP µm.0x50mm InertSustain Phenylhexyl HP µm 4.6x0mm InertSustain Phenylhexyl HP µm 4.6x50mm InertSustain Phenylhexyl HP µm 4.6x75mm InertSustain Phenylhexyl HP µm 4.6x00mm InertSustain Phenylhexyl HP µm 4.6x50mm InertSustain Phenylhexyl HP µm 4.6x50mm 50 * End-fittings are /6 Waters-compatible.

13 Analytical Columns InertSustain Phenylhexyl µm.0x0mm InertSustain Phenylhexyl µm.0x50mm InertSustain Phenylhexyl µm.0x75mm InertSustain Phenylhexyl µm.0x00mm InertSustain Phenylhexyl µm.0x50mm InertSustain Phenylhexyl µm.0x50mm InertSustain Phenylhexyl µm.5x0mm InertSustain Phenylhexyl µm.5x50mm InertSustain Phenylhexyl µm.5x75mm InertSustain Phenylhexyl µm.5x00mm InertSustain Phenylhexyl µm.5x50mm InertSustain Phenylhexyl µm.5x50mm InertSustain Phenylhexyl µm.x0mm InertSustain Phenylhexyl µm.x50mm InertSustain Phenylhexyl µm.x75mm InertSustain Phenylhexyl µm.x00mm InertSustain Phenylhexyl µm.x50mm InertSustain Phenylhexyl µm.x50mm InertSustain Phenylhexyl µm.0x0mm InertSustain Phenylhexyl µm.0x50mm InertSustain Phenylhexyl µm.0x75mm InertSustain Phenylhexyl µm.0x00mm InertSustain Phenylhexyl µm.0x50mm InertSustain Phenylhexyl µm.0x50mm InertSustain Phenylhexyl µm 4.0x0mm InertSustain Phenylhexyl µm 4.0x50mm InertSustain Phenylhexyl µm 4.0x75mm InertSustain Phenylhexyl µm 4.0x00mm InertSustain Phenylhexyl µm 4.0x50mm InertSustain Phenylhexyl µm 4.0x50mm InertSustain Phenylhexyl µm 4.6x0mm InertSustain Phenylhexyl µm 4.6x50mm InertSustain Phenylhexyl µm 4.6x75mm InertSustain Phenylhexyl µm 4.6x00mm InertSustain Phenylhexyl µm 4.6x50mm InertSustain Phenylhexyl µm 4.6x50mm * End-fittings are /6 Waters-compatible.

14 Analytical Columns InertSustain Phenylhexyl 5 µm.0x0mm InertSustain Phenylhexyl 5 µm.0x50mm InertSustain Phenylhexyl 5 µm.0x75mm InertSustain Phenylhexyl 5 µm.0x00mm InertSustain Phenylhexyl 5 µm.0x50mm InertSustain Phenylhexyl 5 µm.0x50mm InertSustain Phenylhexyl 5 µm.5x0mm InertSustain Phenylhexyl 5 µm.5x50mm InertSustain Phenylhexyl 5 µm.5x75mm InertSustain Phenylhexyl 5 µm.5x00mm InertSustain Phenylhexyl 5 µm.5x50mm InertSustain Phenylhexyl 5 µm.5x50mm InertSustain Phenylhexyl 5 µm.x0mm InertSustain Phenylhexyl 5 µm.x50mm InertSustain Phenylhexyl 5 µm.x75mm InertSustain Phenylhexyl 5 µm.x00mm InertSustain Phenylhexyl 5 µm.x50mm InertSustain Phenylhexyl 5 µm.x50mm InertSustain Phenylhexyl 5 µm.0x0mm InertSustain Phenylhexyl 5 µm.0x50mm InertSustain Phenylhexyl 5 µm.0x75mm InertSustain Phenylhexyl 5 µm.0x00mm InertSustain Phenylhexyl 5 µm.0x50mm InertSustain Phenylhexyl 5 µm.0x50mm InertSustain Phenylhexyl 5 µm 4.0x0mm InertSustain Phenylhexyl 5 µm 4.0x50mm InertSustain Phenylhexyl 5 µm 4.0x75mm InertSustain Phenylhexyl 5 µm 4.0x00mm InertSustain Phenylhexyl 5 µm 4.0x50mm InertSustain Phenylhexyl 5 µm 4.0x50mm InertSustain Phenylhexyl 5 µm 4.6x0mm InertSustain Phenylhexyl 5 µm 4.6x50mm InertSustain Phenylhexyl 5 µm 4.6x75mm InertSustain Phenylhexyl 5 µm 4.6x00mm InertSustain Phenylhexyl 5 µm 4.6x50mm InertSustain Phenylhexyl 5 µm 4.6x50mm * End-fittings are /6 Waters-compatible.

15 Guard Columns for UHPLC Holder for Guard Columns for UHPLC - Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.5x0 mm Cartridges, InertSustain Phenylhexyl µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustain Phenylhexyl µm 80 Guard Columns for UHPLC, Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl µm Guard Columns for UHPLC,.5x0 mm, pcs, InertSustain Phenylhexyl µm Guard Columns for UHPLC,.x0 mm, pcs, InertSustain Phenylhexyl µm Guard Columns for UHPLC,.0x0 mm, InertSustain Phenylhexyl µm 80 Cartridge Guard Column Ei (on-metal) Holder for 0 mm, Cartridge Guard Column Ei - Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Holder/Cartridge Set, Holder with pcs.x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Holder/Cartridge Set, Holder with pcs 4.0x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Replacement Cartridges, pcs.0x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Replacement Cartridges, pcs.x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Replacement Cartridges, pcs.0x0 mm, InertSustain Phenylhexyl 5 µm Cartridge Ei Replacement Cartridges, pcs 4.0x0 mm, InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

16 Cartridge Guard Column E Holder for 0 mm, Cartridge Guard Column E Holder for mm, Cartridge Guard Column E Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl µm Holder with pcs.5x0 mm Cartridges, InertSustain Phenylhexyl µm Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl µm Holder with pcs 4.0x0 mm Cartridges, InertSustain Phenylhexyl µm Holder with pcs.0x mm Cartridges, InertSustain Phenylhexyl µm Holder with pcs 4.0x mm Cartridges, InertSustain Phenylhexyl µm Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl 5 µm Holder with pcs.5x0 mm Cartridges, InertSustain Phenylhexyl 5 µm Holder with pcs.0x0 mm Cartridges, InertSustain Phenylhexyl 5 µm Holder with pcs 4.0x0 mm Cartridges, InertSustain Phenylhexyl 5 µm Holder with pcs.0x mm Cartridges, InertSustain Phenylhexyl 5 µm Holder with pcs 4.0x mm Cartridges, InertSustain Phenylhexyl 5 µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl µm Cartridge E Replacement Cartridges, pcs.5x0mm, InertSustain Phenylhexyl µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl µm Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustain Phenylhexyl µm Cartridge E Replacement Cartridges, pcs.0xmm, InertSustain Phenylhexyl µm Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustain Phenylhexyl µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl 5 µm Cartridge E Replacement Cartridges, pcs.5x0mm, InertSustain Phenylhexyl 5 µm Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustain Phenylhexyl 5 µm Cartridge E Replacement Cartridges, pcs 4.0x0mm, InertSustain Phenylhexyl 5 µm Cartridge E Replacement Cartridges, pcs.0xmm, InertSustain Phenylhexyl 5 µm Cartridge E Replacement Cartridges, pcs 4.0xmm, InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

17 GL Cart Guard Column * End-fittings are /6 Waters-compatible Holder for GL Cart Multipurpose Type - GL Cart Holder/Cartridge Set, Holder with 5 pcs Cartridges, InertSustain Phenylhexyl µm GL Cart Replacement Cartridges, 0 pcs 4.6x5 mm, InertSustain Phenylhexyl µm GL Cart Holder/Cartridge Set, Holder with 5 pcs Cartridges, InertSustain Phenylhexyl 5 µm GL Cart Replacement Cartridges, 0 pcs 4.6x5 mm, InertSustain Phenylhexyl 5 µm Conventional Guard Column Guard Column.0Xmm InertSustain Phenylhexyl µm Guard Column.0X50mm InertSustain Phenylhexyl µm Guard Column.5Xmm InertSustain Phenylhexyl µm Guard Column.5X50mm InertSustain Phenylhexyl µm Guard Column.Xmm InertSustain Phenylhexyl µm Guard Column.X50mm InertSustain Phenylhexyl µm Guard Column.0Xmm InertSustain Phenylhexyl µm Guard Column.0X50mm InertSustain Phenylhexyl µm Guard Column 4.0X0mm InertSustain Phenylhexyl µm Guard Column 4.0Xmm InertSustain Phenylhexyl µm Guard Column 4.0X50mm InertSustain Phenylhexyl µm Guard Column 4.6Xmm InertSustain Phenylhexyl µm Guard Column 4.6X50mm InertSustain Phenylhexyl µm Guard Column.0Xmm InertSustain Phenylhexyl 5 µm Guard Column.0X50mm InertSustain Phenylhexyl 5 µm Guard Column.5Xmm InertSustain Phenylhexyl 5 µm Guard Column.5X50mm InertSustain Phenylhexyl 5 µm Guard Column.Xmm InertSustain Phenylhexyl 5 µm Guard Column.X50mm InertSustain Phenylhexyl 5 µm Guard Column.0Xmm InertSustain Phenylhexyl 5 µm Guard Column.0X50mm InertSustain Phenylhexyl 5 µm Guard Column 4.0X0mm InertSustain Phenylhexyl 5 µm Guard Column 4.0Xmm InertSustain Phenylhexyl 5 µm Guard Column 4.0X50mm InertSustain Phenylhexyl 5 µm Guard Column 4.6Xmm InertSustain Phenylhexyl 5 µm Guard Column 4.6X50mm InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

18 Preparative Columns Max. Operating Pressure InertSustain Phenylhexyl 5 µm 6.0x50mm InertSustain Phenylhexyl 5 µm 6.0x00mm InertSustain Phenylhexyl 5 µm 6.0x50mm InertSustain Phenylhexyl 5 µm 6.0x50mm InertSustain Phenylhexyl 5 µm 7.6x50mm InertSustain Phenylhexyl 5 µm 7.6x00mm InertSustain Phenylhexyl 5 µm 7.6x50mm InertSustain Phenylhexyl 5 µm 7.6x50mm InertSustain Phenylhexyl 5 µm 0x50mm InertSustain Phenylhexyl 5 µm 0x00mm InertSustain Phenylhexyl 5 µm 0x50mm InertSustain Phenylhexyl 5 µm 0x50mm InertSustain Phenylhexyl 5 µm 4x50mm InertSustain Phenylhexyl 5 µm 4x00mm InertSustain Phenylhexyl 5 µm 4x50mm InertSustain Phenylhexyl 5 µm 4x50mm InertSustain Phenylhexyl 5 µm x50mm InertSustain Phenylhexyl 5 µm x00mm InertSustain Phenylhexyl 5 µm x50mm InertSustain Phenylhexyl 5 µm x50mm * End-fittings are /6 Waters-compatible. Guard Columns for Preparative Columns * End-fittings are /6 Waters-compatible InertSustain Phenylhexyl 5 µm 6.0x50mm Guard InertSustain Phenylhexyl 5 µm 7.6x50mm Guard InertSustain Phenylhexyl 5 µm 0x50mm Guard InertSustain Phenylhexyl 5 µm 4x50mm Guard InertSustain Phenylhexyl 5 µm x50mm Guard Cartridge Guard Columns for Preparative Columns Holder for Prep Guard Cartridge Prep Guard Cartridges, pcs 7.6x0 mm, InertSustain Phenylhexyl 5 µm Prep Guard Holder/Cartridge Set, Holder with pcs 7.6x0 mm Cartridges, InertSustain Phenylhexyl 5 µm * End-fittings are /6 Waters-compatible.

19 Capillary Columns Capillary EX-AO 0.05X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.05X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.05X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.075X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.075X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm Capillary EX-AO 0.X50mm InertSustain Phenylhexyl µm Capillary EX 0.X50mm InertSustain Phenylhexyl µm Capillary EX 0.X50mm InertSustain Phenylhexyl µm Capillary EX 0.5X50mm InertSustain Phenylhexyl µm Capillary EX 0.5X50mm InertSustain Phenylhexyl µm Capillary EX 0.7X50mm InertSustain Phenylhexyl µm Capillary EX 0.7X50mm InertSustain Phenylhexyl µm EX Micro Guard (Metal) 0.Xmm InertSustain Phenylhexyl µm Capillary EX-AO 0.05x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.05x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.05x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.075x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.075x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.075x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm Capillary EX-AO 0.x50mm InertSustain Phenylhexyl 5 µm Capillary EX 0.x50mm InertSustain Phenylhexyl 5 µm Capillary EX 0.x50mm InertSustain Phenylhexyl 5 µm Capillary EX 0.5x50mm InertSustain Phenylhexyl 5 µm Capillary EX 0.5x50mm InertSustain Phenylhexyl 5 µm Capillary EX 0.7x50mm InertSustain Phenylhexyl 5 µm Capillary EX 0.7x50mm InertSustain Phenylhexyl 5 µm EX Micro Guard (Metal) 0.xmm InertSustain Phenylhexyl 5 µm EX Micro Guard (on-metal) 0.xmm InertSustain Phenylhexyl 5 µm * End-fittings are Valco /6 (0- UF). * Valco / (6-40 UF) end-fittings are available, indicate / when ordering.

Worldwide Ordering Information GL Sciences, Inc. USA 47 Torrance Blvd.

Japan - ishishinjuku 6-Chome Shinjuku-ku, Tokyo, 6-0, Japan Phone: +8--5-66 Fax: +8--5-66 Email: world@gls.co.

De Sleutel 9 565 AS Eindhoven The etherlands Phone: + (0)40 54 95 Fax: + (0)40 54 97 79 Email: info@glsciences.

eu International Distributors Visit our Website at www.glsciences.

20 Worldwide Ordering Information GL Sciences, Inc. USA 47 Torrance Blvd. Suite 55 Torrance, CA 9050 Phone: Fax: Web: GL Sciences, Inc. Japan - ishishinjuku 6-Chome Shinjuku-ku, Tokyo, 6-0, Japan Phone: Fax: world@gls.co.jp Web: GL Sciences B.V. De Sleutel AS Eindhoven The etherlands Phone: + (0) Fax: + (0) info@glsciences.eu Web: International Distributors Visit our Website at The GL Sciences name, the GL Sciences logo and the following registered trademark or trademark are the property of GL Sciences Inc. Inertsil InertSustain InertSustainSwift All other trademarks or service marks are the property of their respective owners. The specification and the column type are subject to change without notice due to continual improvements.

InertSustainBio C18. Rapid Separations of Proteins and Peptides

InertSustainBio C18. Rapid Separations of Proteins and Peptides InertSustainBio C18 Rapid Separations of Proteins and Peptides High Recoveries of Proteins and Peptides The 200A pore size silica creates the opportunity to separate compounds having a molecular weight