Application of two fluorescence methods for detection and quantification of smooth surface carious lesions. Abdulaziz Saad Aljehani

Size: px
Start display at page:

Download "Application of two fluorescence methods for detection and quantification of smooth surface carious lesions. Abdulaziz Saad Aljehani"

Transcription

1 From the Department of Cariology and Endodontology, Institute of Odontology, Karolinska Institutet, Stockholm, Sweden Application of two fluorescence methods for detection and quantification of smooth surface carious lesions Abdulaziz Saad Aljehani Stockholm 2006

2 Application of two fluorescence methods for quantification of smooth surface caries OPPONENT: Professor Jukka Meurman, Department of Cariology, Institute of Dentistry, University of Helsinki, Helsinki, Finland EXAMINING COMMITTEE: Professor Björn Ögaard, Department of Orthodontics, Faculty of Dentistry, University of Oslo, Oslo, Norway Professor Jan Huggare, Department of Orthodontics, Institute of Odontology, Karolinska Institutet, Huddinge, Sweden Associate professor Solveig Fure, Department of Cariology, Faculty of Odontology, Sahlgrenska Academy at Göteborg University, Göteborg, Sweden SUPERVISORS: Dr. Xie-Qi Shi, Department of Cariology and Endodontology, Institute of Odontology, Karolinska Institutet, Sweden Professor Birgit Angmar-Månsson, Department of Cariology and Endodontology, Institute of Odontology, Karolinska Institutet, Sweden Author s address: Dr. Abdulaziz Aljehani 1) Karolinska Institutet 2) Security Force Hospital Institute of Odontology Dept. of Dentistry Dept. of Cariology and Endodontology P.O. Box 5966 Makkah City P.O. Box 4064 Saudi Arabia Huddinge Abdulaziz.Aljehani@hotmail.com SWEDEN Aljehani.Abdulaziz@ki.se ISBN

3 Abdulaziz Aljehani Dedicated to my family who made significant contributions throughout the process of bringing this thesis to completion Ąz z 3

4 Application of two fluorescence methods for quantification of smooth surface caries 4

5 Abdulaziz Aljehani Table of Contents Abstract... 6 List of papers included in the thesis 8 Introduction... 9 Conventional methods for quantification of smooth surface carious lesions 10 Laser fluorescence methods for caries detection and quantification 11 Aims Materials and methods Materials: 19 Diagnostic methods: 20 Results Discussion...28 Conclusions Acknowledgements References

6 Application of two fluorescence methods for quantification of smooth surface caries Abstract Early diagnosis of carious lesions is a key factor in prevention and management of dental caries. With non-invasive, quantitative diagnostic methods it should be possible to detect lesions at an initial stage and subsequently monitor lesion changes over time during which preventive measures are introduced. Conventional methods are inadequate for a modern approach to clinical caries management. The need for objective and quantitative methods for detecting and monitoring progression of carious lesions is therefore obvious. Aims The present thesis is aimed at evaluating two fluorescence devices, DIAGNOdent and QLF for detection and quantification of carious lesions on smooth surfaces under both in vitro and in vivo conditions, with special reference to applications in orthodontic patients. Material and methods Validity of DIAGNOdent and QLF: (papers I, IV) The validity of two fluorescence methods, DIAGNOdent and QLF, was evaluated for the purpose of caries detection and quantification on non-cavitated carious lesions on smooth surfaces. In paper I, the performance of these fluorescence methods was validated by microradiographic and histopathological analyses in order to correlate the two methods with mineral loss and lesion depth. In paper IV, the DIAGNOdent and the DIAGNOdent pen were validated by histopathological analysis in terms of Spearman s rank correlation coefficient. Reliability of DIAGNOdent and QLF: (papers I, II, IV) The reliability of DIAGNOdent and QLF in terms of observer agreement on quantification of enamel caries on smooth surfaces was evaluated and compared under in vitro conditions employing Intra-class correlation coefficient (ICC) (paper I, IV). The inter- and intra-observer agreement of the DIAGNOdent method was also tested under in vivo conditions (paper II). Application of DIAGNOdent as a monitoring tool for evaluation of preventive programs: (paper III) The capacity of DIAGNOdent for monitoring caries progression was evaluated in a clinical study where twelve subjects with 127 test teeth were randomly assigned to two 6

7 Abdulaziz Aljehani caries preventive treatment groups. The lesion changes over a 12 months period were followed by DIAGNOdent. The mean changes of the DIAGNOdent values for the two treatment groups were analyzed by three-way ANOVA. Results In the in vitro studies on comparisons between the fluorescence methods with respect to the correlation with lesion depth, the correlation coefficients for DIAGNOdent and QLF were 0.76 and 0.82, respectively (paper I), and 0.55 and 0.52 for DIAGNOdent and DIAGNOdent pen, respectively (paper IV). The QLF method showed higher correlation with mineral loss, 0.84, compared to 0.64 for DIAGNOdent. The ICC values showed excellent intra-examiner agreement, and good inter-examiner agreement under both in vitro and in vivo conditions. For longitudinal quantification, there was a significant difference in the DIAGNOdent readings between the first and the final evaluation, p= However, the difference between the two treatment groups regarding the changes of DIAGNOdent values over time was not statistically significant, p=0.87. Conclusions It was concluded that the applied fluorescence methods are reliable and valid for quantification of natural carious lesions on smooth surfaces. The results suggested that it might be feasible to use DIAGNOdent for longitudinal monitoring of carious lesions on smooth surfaces. QLF and DIAGNOdent may aid clinical decision making in caries susceptible individuals, such as orthodontic patients. 7

8 Application of two fluorescence methods for quantification of smooth surface caries List of papers included in the thesis This thesis is based on the following four papers, which will be referred to in the text by their Roman numerals: I. Aljehani A, Tranæus S, Forsberg C-M, Angmar-Månsson B, Shi X-Q. In vitro quantification of white spot enamel lesions adjacent to fixed orthodontic appliances using Quantitative Light-induced Fluorescence and DIAGNOdent. Acta Odontol Scand 2004; 62: II. III. IV. Aljehani A, Bamzahim M, Yousif M, Shi X-Q. In vivo reliability of an lnfra red fluorescence method for quantification of carious lesions in orthodontic patients. Oral Health Prev Dent 2006; 4: (in press) Aljehani A, Yousif M A, Angmar-Månsson B, Shi X-Q. Longitudinal quantification of incipient carious lesions in post-orthodontic patients using a fluorescence method. Submitted. Aljehani A, Yang L, Shi X-Q. Quantification of carious lesions on smooth surfaces with laser fluorescence methods DIAGNOdent and DIAGNOdent pen: an in vitro comparative study. Submitted. 8

9 Abdulaziz Aljehani Introduction During the past few decades, major changes have been observed not only in the prevalence of dental caries, but also in the distribution and pattern of the disease in the population (Marthaler, 1990, 2004). Most notably, the rate of lesion progression through the teeth has slowed. The chronic nature of the disease is reflected in slow lesion progression. This offers a window of opportunity for intervention to reverse the loss of mineral or arrest lesion progression, before the development of irreversible damage to the dental hard tissues. In clinical dental practice, the decrease in the rate of lesion progression has led to modification of thresholds for restorative intervention and a change towards a less invasive approach to management of the disease. The changes in prevalence and progression of dental caries may be attributed to the widespread use of fluoride containing dentifrices in combination with changes of diet and oral hygiene (Bratthall et al., 1996; Hänsel Petersson et al., 1996). Smooth surface carious lesions are frequently found among patients with high caries activity, and in orthodontic patients after removal of fixed orthodontic appliances. On removal of the bands and brackets at the end of active orthodontic treatment, clinical examination discloses the presence of these lesions, which may range in severity from incipient, non-cavitated to advanced lesions. While minor lesions may be aesthetically disturbing, advanced lesions may require restorative treatment (Basdra et al., 1996; Gorton and Featherstone, 2003). The prevalence of white spot lesions in patients who get orthodontic treatment is in the range of 50-96% (Gorelick et al., 1982; Øgaard et al., 1988; Gorton and Featherstone 2003; Boersma et al., 2005). This high prevalence is attributed to the presence of brackets, arch wires, ligatures and other orthodontic auxiliaries that complicate conventional oral hygiene measures, which in turn leads to increase the risk for plaque and food retention. With the increase in plaque retention, there is also an increase in the number of micro-organisms such as mutans streptococci and lactobacilli (Lundström and Krasse, 1987a,b; Chang et al., 1999), which are associated with the initiation and further development of dental caries. Enamel demineralization and white spot lesions occur during and sometimes remain after orthodontic treatment (O Reilly and Featherstone, 1985, Årtun and Brobakken, 1986). Studies have reported that visible white spot lesions can develop as quickly as 9

10 Application of two fluorescence methods for quantification of smooth surface caries within only one month after the fitting of a fixed bonded orthodontic appliance (Øgaard et al., 1988, Melrose et al., 1996). This highlights the need for caries risk assessment at the beginning of treatment. Clearly, the best approach during orthodontic treatment is to prevent lesions occurring. It has been concluded that fluoride preparations, oral hygiene instruction and dietary control have the greatest effect on reducing demineralization (Millet et al., 1999). On the basis of these current concepts of the disease process, lesion detection and early intervention, the primary goals of modern clinical management of caries are: to inhibit the initiation of new lesions, to arrest the progression of established lesions and to enhance the natural process of lesion repair by remineralization (Featherstone, 2004). A further goal, reflecting the reality of decision-making in general dental practice, is to recognize irreversible loss of tooth structure early, and to restore it using minimally invasive therapy. It is clear that key factors in implementing treatment based on these principles are the accurate detection of carious lesions and quantification of lesion progression over time. Interpretation of these data, as part of the overall diagnosis, allows the clinician flexibility in selecting intervention appropriate to the individual patient. Conventional methods for quantification of smooth surface carious lesions For several decades, the accepted method for detecting carious lesions in patients, as well as in clinical trials, has been a combination of clinical examination (visual-tactile, light, mirror, and probing) and bitewing radiographs. Lesions on buccal and lingual surfaces are usually detected solely by visual inspection. The appearance of a suspected lesion is judged by its color, white or brown, lesion reflectance, shiny or dull, and surface texture, rough or smooth. Visual inspection is based on subjective evaluations, which may lead to large diagnostic variations among different examiners. Furthermore, this method is qualitative, which restricts its applications, such as for monitoring lesion development and controlling the effectiveness of preventive procedures. The use of the dental explorer to probe enamel is no longer recommended because probing was shown to potentially damage the enamel surface of subsurface carious lesions and even cause cavitation, thereby increasing the rate of progression of a carious lesions (Ekstrand et al., 1987; van Dorp et al., 1988). 10

11 Abdulaziz Aljehani Thus it is necessary and important to look for and test new objective and quantitative methods in order to aid clinicians in detecting and monitoring progression of carious lesions on smooth surfaces. Laser fluorescence methods for caries detection and quantification As a complement to conventional methods, several new diagnostic techniques for the purpose of detection and monitoring progression of early carious lesions have been introduced and tested. Among these, two laser fluorescence methods: quantitative lightinduced fluorescence method, QLF (de Josselin de Jong et al., 1995; Emami et al., 1996; Al-Khateeb et al., 1997, 1998; Tranaeus et al., 2001a,b), and DIAGNOdent (Shi et al., 2000, 2001a,b; Pinelli et al., 2002; Staudt et al., 2004; Mendes et al., 2005) have generated a great deal of research interest in the early detection of carious lesions on occlusal and smooth surfaces. The near infrared fluorescence method - DIAGNOdent Fluorescence is a well-known phenomenon in science and technology. In simple terms, light at one wavelength (excitation wavelength) is absorbed by a substance and emitted as a second longer wavelength (emission wavelength). The auto fluorescence phenomenon of dental hard tissues has been known for a very long time (Benedict, 1928). The cause of baseline fluorescence in sound teeth is still unclear. It might be the result of combining the inorganic matrix with absorbing organic molecules (Hibst et al., 2001). Red light, as well as infrared fluorescence radiation, is less absorbed and scattered by enamel than light of shorter wavelengths, so that it penetrates the tooth more deeply (Hibst et al., 2001). It is therefore possible to measure fluorescence from underlying carious dentin. Hibst and Gall (1998) found that red light induced fluorescence could differentiate between sound and carious tooth tissue. Fluorescence spectroscopic investigations revealed considerable contrast between sound and carious tooth tissues when excited by red light with a wavelength of 655 nm (Hibst et al., 2001). Fluorescence was found to be more intense in carious tissue compared with sound tissue (Figure 1). 11

12 Application of two fluorescence methods for quantification of smooth surface caries Fig. 1. Fluorescence spectra of sound and carious tooth areas when excited by red light, 655 nm, (Lussi et al., J Dent Res 2004). Based on the above mentioned fact, a laser based instrument, DIAGNOdent (KaVo, Biberach, Germany), for the detection and quantification of carious lesions was introduced in 1998 (König et al. 1998; Hibst and Gall, 1998). The main unit of DIAGNOdent is connected to a hand probe by a cable, with descendant and ascendant optical glass fibers. The probe comes with 2 attachments, one with the tapered tip A, intended for occlusal surfaces, and the other with the flat tip B, intended for smooth surfaces (Figure 2). Fig. 2. DIAGNOdent (original version) 12

13 Abdulaziz Aljehani The DIAGNOdent unit contains a laser diode (655nm, modulated, 1 mw peak power) as the excitation light source, and a photo diode combined with a band pass filter (transmission > 680 nm) as the detector. Laser light is generated by the main unit and transmitted through the excitation optical fiber to the tip of the hand piece. Once the tip is in contact with a tooth surface, the laser energy penetrates the tooth surface and is absorbed by the surrounding tooth material, and fluorescence within the infra-red spectrum occurs. The emitted fluorescence, as well as backscattered ambient light, is collected by the tip and carried back to a photo diode detector in the main unit via the detection fibers. The band pass filter absorbs the backscattered excitation and other short wavelength ambient light and transmits the long-wavelength fluorescence radiation. To eliminate the long-wavelength ambient light also passing through the filter, the laser diode is modulated, and only light showing the same modulation characteristic is registered by the main unit and displayed as nominal values ranging from 0 to 99, where 0 indicates minimum and 99 maximum fluorescence. The principle of DIAGNOdent is presented in Figure 3. Fig. 3. The DIAGNOdent principle (Lussi et al., J Dent Res 2004). Hibst and Gall (1998) reported that carious tissue emits stronger fluorescence than sound tissue in the red and infrared part of the spectrum (λ= 655 nm). Thus, the fluorescence from a carious region, greater than that from sound tissue, is expressed as a higher numerical readout by the device. The exact mechanism of detection has not been 13

14 Application of two fluorescence methods for quantification of smooth surface caries fully clarified; however, one assumption is that the device measures the fluorescence of bacterial disintegrated products within carious tissues, possibly porphyrins (Hibst et al., 2001). Recently a new version of DIAGNOdent, the DIAGNOdent pen, was introduced. This new device is based on the same principle as the original DIAGNOdent. The main unit measures 21 cm with two sapphire tips, wedge shaped and tapered shaped. The thickness of the wedge shaped tip is only 0.4 mm with a width of 1.1 mm in order to improve the access to proximal surfaces whereas the thickness of the tapered shaped tip is 0.7 mm. The device is cordless and handy to operate (Figure 4). Fig. 4. The DIAGNOdent pen The DIAGNOdent device has been studied extensively both in vivo and in vitro for detection of carious lesions for occlusal and smooth surface caries detection (for a review see Bader and Shugars, 2004). Recent studies by Anttonen et al, (2003, 2004) and Sköld-Larsson et al, (2004) support the application of the method for detection and monitoring of fissure caries. Recently DIAGNOdent has been tested in vitro for quantification of lesions adjacent to fixed orthodontic appliances (Staudt et al., 2004). The results suggested that the DIAGNOdent method might be appropriate for early detection and assessment of white spot lesions in orthodontic patients. To our knowledge there are to date no published studies evaluating the clinical application of DIAGNOdent for detection and monitoring of white spot lesions on smooth surfaces in orthodontic patients following fixed appliance therapy. To achieve the maximum extension of the lesion volume, one must tilt the instrument around the measuring site with continuous rotation of the probe tip around its axis during the measuring period. This ensures that the tip picks up fluorescence from all 14

15 Abdulaziz Aljehani directions. There are several factors that need to be taken into consideration when using DIAGNOdent. The manufacturer recommends that all measurements should be made at room temperature of about 22º C and the tooth surfaces should be dry. Calibration of the device against the ceramic standard before the measurement session is important to ensure accurate measurements over an extended period of time (Karlsson et al., 2004). It is also essential to perform the measurements on a clean tooth because the instrument is very sensitive to the presence of stain, deposits, calculus, as well as certain types of composite filling material, and remnants of pastes, which may produce fluorescence and therefore, cause false-positive readings (Lussi et al., 1999, 2005; Shi et al., 2000). Further, potential sources of error should be considered such as storage media for extracted teeth, which may affect the DIAGNOdent values and make it difficult to directly apply cut-off values obtained from in vitro studies to values under clinical conditions (Shi et al., 2001b). QLF: Quantitative laser/light-induced fluorescence In the beginning of 1980s laser light was used to induce auto fluorescence of enamel, and was employed to develop a sensitive, non-destructive diagnostic method for detection of enamel demineralization and dental caries (Bjelkhagen et al., 1982). Since then this method has been developed, validated and applied to in vitro, in situ and in vivo studies (Hafström-Björkman et al., 1992; de Josselin, de Jong et al., 1995; Emami et al., 1996, Al-Khateeb et al., 1997a,b 1998; Tranaeus et al., 2001a). The QLF equipment comprises a light source that generates non-coherent light from a xenon lamp provided with an optical band pass filter with a peak intensity of 370 nm in order to produce violet-blue light. A CCD based digital camera and a PC are also included in the equipment. When violet-blue light illuminates the tooth surface, a digital fluorescent image is captured by the camera, transferred to the computer and displayed on the monitor simultaneously. Data are collected, stored, and analyzed by custommade software (Figure 5) The QLF method is based on the principle that mineral loss, caused by carious destruction of tooth enamel, can be detected and measured as a decrease in fluorescence intensity when exposed to violet-blue light. The fluorescence of a carious lesion viewed by QLF is lower than that of sound tissue and thus appears as a dark area in a QLF 15

16 Application of two fluorescence methods for quantification of smooth surface caries image. Several effects may contribute to a decreased fluorescence of incipient carious lesions; the most probable are the following: firstly, light scattering in a lesion, which is much stronger than in sound enamel (Angmar-Månsson and ten Bosch, 2001), causes the light path in the lesion to be shorter than in sound enamel, thus the absorption per volume unit is small in the lesion and the fluorescence will become low. Secondly, light scattering in the lesion acts as a barrier, blocking excitation light from reaching the underlying fluorescent dentine, and also as a barrier to fluorescence light from dentine from reaching the tooth surface. Fig. 5. The QLF principle (Stookey, Dent Clin North Am 2005) There is a relation between mineral loss and fluorescent radiance, i.e. decreasing fluorescence with increasing mineral loss (Emami et al., 1996; Al-Khateeb et al., 1997 a, b). To enable calculation of loss of fluorescence in the carious lesion, the fluorescent radiance of sound tissue at the lesion site is reconstructed by interpolation from the radiance of the sound tissue surrounding the lesion (de Josselin de Jong et al., 1995). The difference between the measured values and the reconstructed values gives the resulting fluorescence loss in the lesion. Studies have shown that QLF is a sensitive method for detecting and monitoring carious lesions of smooth surfaces (for review see Stookey, 2005). With application of the QLF method, clinical studies intended to assess the effectiveness of caries preventive measures took less time and were hence cheaper. The QLF not only provides quantitative data but also displays the fluorescence image of the tooth on the monitor and this is highly instructive for demonstrating lesion progression and /or regression to 16

17 Abdulaziz Aljehani the patient with no danger to operator or patient. However, a quantitative evaluation can only take place if a variety of confounding factors are controlled. As the surface of the tooth or the lesion may be covered by dental plaque, calculus, and/or extrinsic discolorations, professional cleaning prior to QLF recording is essential. The effect of dehydration is a potential source of error for in vitro studies using QLF. There are also other reasons for measurement errors, including examiner effects, subject effects and imperfect calibration. The daylight or office light may influence the quality of the QLF image. The method does not differentiate between active and arrested carious lesions or between carious lesions and developmental defects with lower mineral content. If gingivitis exists, gum bleeding and/or an increased sulcus fluid flow rate may interfere with both the capture of fluorescence images and the following analysis. It was noted that the orientation of the camera when capturing the QLF image was important in visualizing different aspects of the tooth surface (Benson et al., 2003). The equipment is still expensive, but will probably become less expensive in the future. 17

18 Application of two fluorescence methods for quantification of smooth surface caries Aims General aim The present thesis aimed at evaluating two fluorescence devices, DIAGNOdent and Quantitative Light-Induced Fluorescence method (QLF) for detection and quantification of carious lesions on smooth surfaces in vitro and in vivo. Specific aims 1. To validate the DIAGNOdent method in vitro and compare it with Quantitative Light-induced Fluorescence (QLF) to enable quantification of white spot lesions adjacent to orthodontic brackets (Paper I). 2. To compare the reliability of DIAGNOdent with the QLF method in terms of inter-observer agreement of in vitro quantification of enamel lesions adjacent to orthodontic brackets (Paper I). 3. To evaluate the reliability of DIAGNOdent in terms of intra- and inter-observer agreement, enabling quantification of white spot lesions in orthodontic patients subsequent to fixed appliance therapy (Paper II). 4. To monitor changes in incipient carious lesions that had been formed around orthodontic bands and brackets in vivo, using DIAGNOdent (Paper III). 5. To evaluate the effect of two preventive programs on regression of incipient carious lesions that had been formed in vivo around orthodontic bands and brackets during orthodontic treatment (Paper III). 6. To compare the validity and reliability of the newly introduced DIAGNOdent pen with those of the original DIAGNOdent device used for in vitro quantification of carious lesions on smooth surfaces (Paper IV). 18

19 Abdulaziz Aljehani Materials and methods All four studies were approved by the Ethics Committee at Huddinge University Hospital, Huddinge, Sweden (124/02, 60/03, and 564/03). Materials: Extracted teeth The material used in the in vitro studies is summarized in Table 1. In Papers I and IV, the material comprised premolar teeth that had been extracted for orthodontic reasons. The teeth were thoroughly rinsed under tap water and cleaned with a toothbrush. Paper Teeth n Visual appearance I 41 visually sound; incipient caries IV 52 visually sound; non-cavitated carious lesions Examined site smooth surface around bracket margin smooth surface Equipment DIAGNOdent QLF DIAGNOdent DIAGNOdent pen Table 1. Study material In paper I, orthodontic brackets (Ormco with metal composition of 8.6% Ni, 0.1% Mo, 69.7% Fe, 18.5% Cr and Si+) were cemented with regular orthodontic composite cement in contact with the carious lesions simulating the position of brackets under clinical situation as demonstrated in Figure 6. Precaution was taken to avoid excess cement along the bracket, close to the lesion. The teeth in Papers I, and IV were numbered and stored under refrigeration in thymolsaturated saline, in individual plastic containers. 19

20 Application of two fluorescence methods for quantification of smooth surface caries Fig. 6. Tooth specimens cemented with orthodontic bracket under ordinary light Patients In Papers II, 137 test teeth from 13 patients with ages between 13 and 17 years were recruited from King Abdulaziz Hospital, Makkah, Saudi Arabia. The subjects were selected from orthodontic patients who had recently completed full fixed appliance therapy with an average treatment time of two years. In Paper III, 127 test teeth from 12 patients exhibiting white spot lesions on the buccal surfaces were obtained from 12 subjects ranging in ages from 13 to 17 years. Diagnostic methods: Quantitative light-induced Fluorescence: Paper I The QLF device (QLF 1.98i, Inspektor Research System BV, Amsterdam, the Netherlands) employed in this study consisted of a camera (Panasonic WV-KS 152) with non-coherent light from a xenon lamp as the light source combined with a filter system. A schematic drawing of the experimental setup is presented in Figure 7. QLF images of 41 smooth surfaces were captured with focus on the lesion and the edge of the bracket base. Reconstruction of the sound fluorescence of the lesion areas was then performed as follows; the patch line used as reference for reconstruction of the demineralized region was set along the sound tissue close to the lesion. One border of the reconstruction patch nearest to the bracket was excluded due to lack of sound tissue between lesion and bracket. A satisfactory reconstruction was obtained by visual evaluation of the reconstructed images. Mean fluorescence loss in the lesion ( F) was calculated and registered. The QLF images of each tooth surface were printed out as reference images to facilitate measurements with DIAGNOdent later on. 20

21 Abdulaziz Aljehani Fig. 7. Experimental set up for the QLF measurement DIAGNOdent: Papers I, II, III, IV Before starting the measurements with DIAGNOdent, each measuring site was documented with either a digital photograph (Papers II, III, IV) or with a QLF image (Paper I). As recommended by the manufacturer, prior to every measurement session, each DIAGNOdent instrument was calibrated against its own supplied ceramic standard. First the standard value for each tooth was calibrated by measuring a sound site on the buccal surface. The lesion area or the visually sound surfaces were carefully scanned with the probe by holding the tip in contact with tooth surface and tilting the tip around the measuring site in order to collect the fluorescence from all directions. The maximum readings and the corresponding measuring sites in the picture were registered to facilitate the follow-up measurement or/and subsequent preparation of the tooth slice for histopathological analysis. Measuring details are listed in Table 2 for the four studies. Paper Condition Tip Measuring Humidity Environment time I in vitro conical 10 s dry 5 s thymol II in vivo flat 10 s dry 5 s oral cavity III in vivo flat 10 s dry 5 s oral cavity IV in vitro flat 10 s dry 5 s thymol Table 2. Measuring details 21

22 Application of two fluorescence methods for quantification of smooth surface caries Figure 8 illustrates the experimental setup of the DIAGNOdent measurement (Papers I, IV) and a clinical picture of white spot lesions (Papers II, III) after removal of orthodontic brackets.. Fig. 8. a. Experimental setup of the DIAGNOdent measurement; b. An example of a lesion in studies II, III, after removal of orthodontic brackets Before the measurement with DIAGNOdent in Paper I, a pilot study on 5 extracted teeth with orthodontic brackets was carried out to test whether the bracket emits fluorescence when using DIAGNOdent. Individual calibration was done on a sound spot and measurement was performed on the metal bracket. It was found that the metal orthodontic bracket did not give any fluorescent signal and thus had no effect on the DIAGNOdent readings. Visual inspection: Papers II, III Visual examination was included in the clinical investigations, i.e. Papers II and III. Three examiners, two cariologists and one orthodontist, worked together to localize a lesion and to perform caries assessment. In case of disagreement, consensus was reached. Clinical caries assessment was performed by the same observers with the help of mouth mirrors and blunt probes under clinical lighting, according to the modified Ekstrand criteria and classification presented in Table 3 (Ekstrand et al., 1997). The inclusion criterion was the presence of a white spot lesion on a smooth surface adjacent to the site of the orthodontic band or bracket. 22

23 Abdulaziz Aljehani Code Criteria 0 No or slight change in enamel translucency after prolonged air drying. 1 Opacity (white) hardly visible on the wet surface, but distinctly visible after air-drying 2 Opacity (white) distinctly visible without air-drying. 3 Localised enamel breakdown in opaque or discolored enamel and or greyish discoloration from the underlying dentine. Table 3. Visual inspection criteria (modified Ekstrand criteria) Validation of the diagnostic methods: (Papers I, IV) The in vitro performance of the two laser fluorescence methods was validated by histopathological analysis (Papers I, IV) and transverse micro radiography (Paper I) to determine the lesion depth and mineral loss within the lesions, respectively. Histopathological analyses At the end of the experiments in Papers I and IV the teeth were prepared for histopathological examination, which served as gold standard in the validation of lesion depth. Each tooth was embedded in methyl-metacrylate and sectioned with a watercooled diamond saw perpendicular to the occlusal surface in a bucco-lingual or mesiodistal direction at the test sites indicated on the pictures for the DIAGNOdent measurements. Tooth slices, approximately 300 µm thick, were obtained and examined under microscope at x16 magnification. The following point scale was used to stratify the sites according to the histopathological appearance of demineralization: 0 = sound; 1 = enamel caries limited to the outer half of enamel; 2 = caries extending into the inner half of the enamel, but not to the dentino-enamel junction (DEJ). 3 = caries penetrating the DEJ but limited to the outer half of the dentin; 4 = caries involving the inner half of the dentin. Microradiographic analyses For the validation of mineral loss in Paper I, quantitative transverse micro radiography (TMR) was employed to analyze the correlation between the mineral loss in the lesions on one hand, and the QLF and DIAGNOdent reading on other. After histological 23

24 Application of two fluorescence methods for quantification of smooth surface caries analysis, the tooth slices of 300 µm thickness, were manually ground to µm thickness and analysed with transverse micro radiography (TMR) according to the method described by de Josselin de Jong et al. (1987) for microradiographic analysis. The microradiographic image of each tooth slice and an aluminum step wedge for calibration, were recorded on holographic film (Kodak SO-253) exposed to Ni-filtered Cu Kα radiation at 20 kv and 55 ma, with an exposure time of 20 s. The film was developed according to the manufacturer s instruction. Mineral content depth profiles were recorded with a microscope-densitometer-computer set-up equipped with a Ikegami CCD camera and software for TMR 2000 (Inspektor Research Systems BV, Amsterdam, the Netherlands), using the densitometric values to calculate the mineral content according to the formula of Angmar et al. (1963). The tracings were expressed as integrated mineral loss ( Z), using the established parameter definition (Arends and ten Bosch, 1992). The mean integrated mineral loss from three scans was obtained for each specimen. Reliability of the diagnostic methods: (Papers I, II, IV) Once the measuring procedures of both methods had been established and carried out by the first operator, the measurements were repeated after a few days by the same and/or another investigator. Detailed information such as the number of teeth and observers involved in each study is listed in Table 4. For studying the reproducibility of the QLF method (paper I) 3 sets of new images were acquired and analyzed by each operator, respectively. To determine the reliability of DIAGNOdent (Papers II, IV), the preselected measuring sites documented by digital photographs were scanned under identical conditions. The DIAGNOdent reading was noted and the corresponding site was registered on the photographs. After a 1-week interval, the same measuring procedures were repeated by the same observers. The observers again took independent readings of the same sites using the photographs as a guide. No records of the DIAGNOdent readings from the first session were available during the second measurement session. On conclusion of all measurements with the diagnostic methods, intra-and inter-observer agreements were analysed. 24

25 Abdulaziz Aljehani Paper Condition Samples Pretraining Observers Evaluation I in vitro 20 no 4 inter-observer II in vivo 137 yes 3 intra-and interobserver IV in vitro 52 yes 2 intra-and interobserver Table 4. Reliability studies Application of DIAGNOdent as a monitoring tool for evaluation of preventive programs: (Paper III) Following completed orthodontic therapy the twelve subjects with 127 test teeth exhibiting white spot lesions on the buccal surfaces were randomly divided into two treatment groups. The experimental group received professional teeth cleaning combined with oral hygiene instruction, while the comparison group received oral hygiene instruction only. The pre-selected measuring sites were measured by DIAGNOdent and at baseline 3, 6, 9, and 12 months. The numerical readings given by the DIAGNOdent were recorded in a case report form (CRF). No records of the DIAGNOdent readings from the previous measurement sessions were available at the follow up measurement sessions. Statistical analyses Validity: Correlation coefficient Spearman s Rank correlation coefficient was performed to analyze the correlation between the gold standard for lesion depth determined by histopathology and the readings of the diagnostic methods in Papers I, IV. The correlation between the integrated mineral loss and the mean fluorescence loss determined by QLF and the DIAGNOdent readings (Paper I), as well as the correlation between DIAGNOdent and the DIAGNOdent pen (Paper IV) was analyzed using Pearson s correlation coefficient. Mean changes of the DIAGNOdent values during five visits over a period of one year for the two treatment groups in Paper III were analyzed with a three-way ANOVA with repeated measures of two factors. The within factors are time (five time points) and 25

26 Application of two fluorescence methods for quantification of smooth surface caries quadrant (four levels) and the between factor is treatment group (OHI, and OHI plus PTC). Statistically significant difference was accepted at P < Reliability: inter-and intra-observer agreement Intra-class correlation coefficient (ICC) was used to evaluate the level of agreement between and within observers in the two measurement sessions for both in vitro and in vivo comparisons. Results Validity of the diagnostic methods The distribution of teeth with respect to lesion depth in the in vitro studies is listed in Table 5. Paper I Paper IV n % n % Sound Lesions involving the outer half of enamel Lesions involving the inner half of enamel Lesions involving the outer half of dentin Lesions involving the inner half of dentin Total Table 5. Results from histopathology: number (n) and percentage (%) of sound and carious teeth. In the in vitro studies with comparison between the fluorescence methods and lesion depth, Spearman s rank correlation coefficient for DIAGNOdent and QLF were 0.76 and 0.82, respectively, whereas the Pearson s correlation coefficient between mineral loss as measured with TMR and readings from the two methods were 0.64 and 0.84, respectively (Paper I). In Paper IV, the correlations with histology were 0.55 and 0.52 for DIAGNOdent and the DIAGNOdent pen, respectively, whereas the Pearson s correlation coefficient between DIAGNOdent and the DIAGNOdent pen at the first and second measurement sessions were 0.92 and 0.96, respectively. 26

27 Abdulaziz Aljehani Reliability of the diagnostic methods In Paper I, the inter-observer agreement between the four operators on measurements of enamel carious lesions in connection with orthodontic brackets were 0.80 and 0.93 for DIAGNOdent and QLF, respectively. In Paper IV, the ICC values for intra-observer agreements were excellent for both DIAGNOdent and the DIAGNOdent pen, 0.98, whereas the inter-observer agreements were relatively lower, 0.70 and 0.68, for the DIAGNOdent and DIAGNOdent pen, respectively. In Paper II, under in vivo conditions, the reliability of DIAGNOdent was excellent in terms of intra-examiner agreement, 0.95, and the inter-examiner agreement was good, 0.69 and 0.82 for the first and second measurements, respectively. Evaluation of preventive programs using DIAGNOdent as a monitoring tool In Paper III, for longitudinal quantification of carious lesions on smooth surfaces, there was a significant difference in the DIAGNOdent readings between the first and the final evaluation, p=0.025, However, the difference between the two treatment groups regarding changes of the DIAGNOdent values over time was not statistically significant, p=0.87. Spearman s rank correlation coefficient between the visual scores and the DIAGNOdent readings was The differences between the visual scores of the baseline and those of the final examination were not statically significant, p=

28 Application of two fluorescence methods for quantification of smooth surface caries Discussion Appropriate diagnosis of carious lesions at their earliest stages is a major factor in prevention and management of dental caries. Carious lesions on smooth surfaces are usually visually detected first as white spot lesions. However, visual inspection alone is inadequate to monitor changes of a lesion over time. Thus objective and quantitative diagnostic methods would improve the potential for appropriate clinical caries management. The studies on which this thesis is based were designed to evaluate the application of fluorescence methods in order to aid the quantification of smooth surface carious lesions. The methods were evaluated with special reference to their application to the teeth of orthodontic patients, where the risk of developing carious lesions is high. The applications of these methods were not only in detection and quantification of lesions on smooth surfaces, but also for monitoring lesion changes following preventive intervention. The reliability of the two fluorescence methods, in quantification of smooth surface caries in terms of agreement within and between operators, was also evaluated. In the present thesis the primary aim was to evaluate the two fluorescence devices, DIAGNOdent and the quantitative light-induced fluorescence method (QLF) used in in vitro detection and quantification of carious lesions on smooth surfaces adjacent to fixed orthodontic brackets simulating clinical situations. The methods were validated by two well established methods as gold standard; histopathology and transverse microradiography, used here in the determination of lesion depth and mineral loss. The correlation analyses showed that the associations between the two fluorescence methods and lesion depth were comparable (0.82 and 0.76), indicating good correlations with gold standard. Similar correlation with lesion depth for the two methods was also found in earlier studies (Shi et al., 2001a, b). When validated by TMR for mineral loss the QLF method had higher correlation coefficient (r=0.84) than the DIAGNOdent (r=0.64). This implies that QLF is a better method for evaluating mineral changes in the enamel lesions, a finding which is in agreement with the results from previous studies (Al-Khateeb et al., 1998, Shi et al., 2001a, Benson et al., 2003, Pretty et al., 2003). The higher correlation with TMR for the QLF method could be expected since the 28

29 Abdulaziz Aljehani mechanisms behind the function of the QLF method are more directly related to the changes in mineral content, whereas in the DIAGNOdent method the increase of fluorescence due to the presence of caries is related to bacterial metabolites, rather than to crystalline disintegration (Hibst and Paulus, 1999). However, DIAGNOdent compared well with QLF in respect of correlation with lesion depth for quantification of natural carious lesions on smooth surfaces, while QLF showed closer correlation with mineral changes, and is therefore the method of preference for scientific purposes. The QLF method has many merits, however, because of its hard- and software components it is rather an expensive technique. For dentists who are not familiar with image reconstruction software it may be cumbersome in the conventional dental setting. Further more, another major disadvantage of QLF is related to technical limitations of the method, namely the technique can not be applied to any white spot lesion on the smooth surface in the orthodontic patient, e.g. if the lesion is situated close to the gingiva and/or to the orthodontic bracket, the reconstruction of the sound fluorescence becomes rather difficult due to insufficient sound tissue between lesion, bracket and gingiva. A less expensive, more compact device such as DIAGNOdent, would probably find greater acceptance by clinicians to use in dental practice for longitudinal monitoring of lesion response to preventive measures in their caries risk patients. For clarification, in the present thesis, the DIAGNOdent method was selected for further investigations in possible application for scientific purpose, such as evaluation of preventive programs. In this context, the DIAGNOdent method was applied as a monitoring tool to evaluate the effect of two preventive programs on incipient carious lesions, which had been formed in vivo around orthodontic bands and brackets during orthodontic treatment. As mentioned before an important feature of a diagnostic method is to provide consistent and standardized measurements between and within observers. In Paper II, the reliability of DIAGNOdent was evaluated in orthodontic patients under clinical conditions, in order to determine whether the results of the in vitro study (Paper I) discussed above were reproducible, and with respect to possibly affecting variables under clinical conditions. Excellent intra-examiner agreement, 0.96, was found with in vivo quantification of white spot lesions on smooth surfaces, which is in good agreement with previous reports by Shi et al., (2001b) under in vitro conditions, and 29

30 Application of two fluorescence methods for quantification of smooth surface caries under in vivo conditions (Pinelli et al., 2002). The inter-observer agreement of 0.75 is in general agreement with the findings of Paper I, and with those from a recent in vitro study on smooth surface caries (Pinelli et al., 2002). However, less experienced operators would probably need increased training to obtain the necessary skills in the technique. These findings of high reliability of DIAGNOdent indicate consistent performance. Thus, the method may be suitable for longitudinal monitoring of carious lesions and thus aid clinical decision making with respect to management of orthodontically induced smooth surface lesions, provided that DIAGNOdent is sensitive enough to detect small changes in carious lesions over time. To test the applicability of DIAGNOdent for monitoring caries progression, DIAGNOdent was applied to in vivo longitudinal assessment of white spot lesions over a period of one year. During this year the effect of two intervention programs intended to arrest or reverse lesion progression were compared using the DIAGNOdent registrations (Paper III). All the lesions were also examined by conventional visual inspection during the de-bonding visit and 12 months thereafter. The differences between the visual scores of the baseline and those at the final examination were not statistically significant, p=0.8. In other words, the progression/regression of the white spot lesions during a period of one year could not be registered by means of visual inspection. A similar finding was reported in a previous study (Heinrich-Weltzien et al., 2005) which demonstrated a weakness in visual inspection for detection of smooth surface initial carious lesions. Although improvements in visual inspection with new scoring systems seem promising (Ekstrand et al. 1997; Nyvad et al., 1999; Fyffe et al., 2000), further clinical validation is still necessary. Unlike the findings from visual inspection, the DIAGNOdent readings showed statistically significant differences between the first and the final evaluations (p=0.025). Generally, the mean DIAGNOdent values for all teeth had decreased by the final evaluation, reflecting regression of the carious lesions. DIAGNOdent thus offered possible means of detecting and quantifying minor changes that had occurred during the study period of one year. These findings confirm the results from a previous study applying the QLF method (Al-Khateeb et al., 1998). 30

31 Abdulaziz Aljehani In Paper III, none of the lesions had fully recovered after one year. The white spot lesions formed around the brackets during orthodontic treatment might possibly be remineralized over a relatively long period of time. As was reported in a previous study (Øgaard et al., 1989), white spot lesions were found to be present 5 years after the completion of orthodontic treatment. Fluoride per se can not prevent caries development if the cariogenic challenge is unchanged (Holmen et al., 1986). Good oral hygiene and improved dietary habits are essential prerequisites to initiate the healing process of the white spot lesions (Årtun and Thylstrup, 1986, 1989; Holmen et al., 1987). Following these principles, no excessive topical fluoride in addition to the recommended fluoride toothpaste was supplied (Paper III). The degree of remineralization was associated with a reduction of the cariogenic and microbial challenge by oral hygiene instruction and professional teeth cleaning. However, in our study (Paper III), there was no statistically significant difference between the effects of the two preventive programs regarding changes of the DIAGNOdent values over time. The combination of professional teeth cleaning and oral hygiene instruction had the similar efficacy as oral hygiene instruction only on promoting re-mineralization of white spot lesions. However, it should be further explored whether long term or more frequent application of professional tooth cleaning might have a significant effect on the re-mineralization of white spot lesions. In Paper IV, the performance of two different DIAGNOdent devices for quantification of smooth surface carious lesions were compared. The results showed that both DIAGNOdent and the DIAGNOdent pen had excellent intra-observer agreements and moderate inter-observer agreements. The conformity on the device level was also tested where excellent agreement was found between the two generations of DIAGNOdent device. Although the DIAGNOdent pen performed equally well as DIAGNOdent for quantification of carious lesions on smooth surfaces using histology as gold standard, more in vitro and in vivo studies with larger samples need to be carried out before any recommendation can be given with regard to the validity of the device. Possible application of the DIAGNOdent pen for scientific purposes, such as evaluation of preventive programs, and to what extent the changes of a lesion can be reliably detected needs however, to be further explored under clinical conditions. 31

Diagnodent and the caveats of caries diagnosis by laser fluorescence

Diagnodent and the caveats of caries diagnosis by laser fluorescence Diagnodent and the caveats of caries diagnosis by laser fluorescence Introduction Professor Laurence Walsh School of Dentistry and Centre for Laser Science, The University of Queensland The Diagnodent

More information

Original Article. Naif A. Almosa a ; Ted Lundgren b ; Abdullah M. Aldrees c ; Dowen Birkhed d ; Heidrun Kjellberg e

Original Article. Naif A. Almosa a ; Ted Lundgren b ; Abdullah M. Aldrees c ; Dowen Birkhed d ; Heidrun Kjellberg e Original Article Diagnosing the severity of buccal caries lesions in governmental and private orthodontic patients at debonding, using the ICDAS-II and the DIAGNOdent Pen Naif A. Almosa a ; Ted Lundgren

More information

Effects of orthodontic treatment with a fixed appliance on the caries experience of patients with high and low risk of caries

Effects of orthodontic treatment with a fixed appliance on the caries experience of patients with high and low risk of caries Journal of Dental Sciences (2011) 6, 195e199 Available online at www.sciencedirect.com journal homepage: www.e-jds.com ORIGINAL ARTICLE Effects of orthodontic treatment with a fixed appliance on the caries

More information

Near-UV light detection

Near-UV light detection C L I N I C A L Near-UV light detection Javier Tapia Guadix 1 Near-UV light induced fluorescence has already proven to be very useful as an alternative to classic caries-detector dyes. However its potential

More information

Performance of a laser fluorescence device in detecting oclussal caries in vitro

Performance of a laser fluorescence device in detecting oclussal caries in vitro Performance of a laser fluorescence device in detecting oclussal caries in vitro Roxana Oancea1, Ruxandra Sava-Roºianu 1, Atena Gãluºcan 2, Daniela Jumanca 3, Ramona Amina Popovici 1, Angela Codruþa Podariu

More information

Caries lesions on smooth surfaces are commonly

Caries lesions on smooth surfaces are commonly ORIGINAL ARTICLE Effect of fluoridated chewing sticks (Miswaks) on white spot lesions in postorthodontic patients Hosam A. Baeshen, a Peter Lingstr om, b and Dowen Birkhed c G oteborg, Sweden Introduction:

More information

Use of the ICDAS system and two fluorescence-based intraoral devices for examination of occlusal surfaces

Use of the ICDAS system and two fluorescence-based intraoral devices for examination of occlusal surfaces A. Theocharopoulou, M.D. Lagerweij, A.J.P. van Strijp Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands e-mail: katerina5the@gmail.com

More information

DIAGNOdent Measurements and Correlation with the Depth and Volume of Minimally Invasive Cavity Preparations

DIAGNOdent Measurements and Correlation with the Depth and Volume of Minimally Invasive Cavity Preparations Operative Dentistry, 2006, 31-3, 291-296 Clinical Research DIAGNOdent Measurements and Correlation with the Depth and Volume of Minimally Invasive Cavity Preparations JC Hamilton WA Gregory JB Valentine

More information

Preprint. Reference. Caries diagnosis using light fluorescence devices: VistaProof and DIAGNOdent. BETRISEY, Emilie, et al.

Preprint. Reference. Caries diagnosis using light fluorescence devices: VistaProof and DIAGNOdent. BETRISEY, Emilie, et al. Preprint Caries diagnosis using light fluorescence devices: VistaProof and DIAGNOdent BETRISEY, Emilie, et al. Reference BETRISEY, Emilie, et al. Caries diagnosis using light fluorescence devices: VistaProof

More information

Exploring the boundaries of caries detection

Exploring the boundaries of caries detection From the Divisions of Cariology and Endodontology, Department of Dental Medicine Karolinska Institutet, Stockholm, Sweden Exploring the boundaries of caries detection Two advanced methods evaluated Álfheiður

More information

A Comparison of Methods for the Detection of Smooth Surface Caries

A Comparison of Methods for the Detection of Smooth Surface Caries A Comparison of Methods for the Detection of Smooth Surface Caries B. Wong 1, K. Sivagurunathan 1,J. D. Silvertown 1, W. M. P. Hellen 2, G. I. Elman 2, S. H. Abrams 1, L. O. Okoye 3, B. T. Amaechi 4 1

More information

The Effect of Mineralizing Fluorine Varnish on the Progression of Initial Caries of Enamel in Temporary Dentition by Laser Fluorescence

The Effect of Mineralizing Fluorine Varnish on the Progression of Initial Caries of Enamel in Temporary Dentition by Laser Fluorescence American Journal of Engineering Research (AJER) e-issn: 2320-0847 p-issn : 2320-0936 Volume-6, Issue-9, pp-39-43 www.ajer.org Research Paper Open Access The Effect of Mineralizing Fluorine Varnish on the

More information

Detection of intraoral lesions using a fluorescence camera

Detection of intraoral lesions using a fluorescence camera Detection of intraoral lesions using a fluorescence camera Michael Thoms Dürr Dental GmbH & Co KG, Höpfigheimer Str. 17, D-74321 Bietigheim-Bissingen University of Erlangen-Nürnberg, Institute of Material

More information

Pilot clinical study to assess caries lesion activity using quantitative lightinduced fluorescence during dehydration

Pilot clinical study to assess caries lesion activity using quantitative lightinduced fluorescence during dehydration Pilot clinical study to assess caries lesion activity using quantitative lightinduced fluorescence during dehydration Masatoshi Ando Andrea G. Ferreira-Zandoná George J. Eckert Domenick T. Zero George

More information

Assessing Caries Removal by Undergraduate Dental Students Using Quantitative Light- Induced Fluorescence

Assessing Caries Removal by Undergraduate Dental Students Using Quantitative Light- Induced Fluorescence Assessing Caries Removal by Undergraduate Dental Students Using Quantitative Light- Induced Fluorescence Adejumoke A. Adeyemi, Ph.D.; Fadi D. Jarad, Ph.D.; Gleb N. Komarov, Ph.D.; Neil Pender, Ph.D.; Susan

More information

Examination and Treatment Protocols for Dental Caries and Inflammatory Periodontal Disease

Examination and Treatment Protocols for Dental Caries and Inflammatory Periodontal Disease Examination and Treatment Protocols for Dental Caries and Inflammatory Periodontal Disease Dental Caries The current understanding of the caries process supports the shift in caries management from a restorative-only

More information

Original Paper. D.F. Côrtes a K.R. Ekstrand b A.R. Elias-Boneta c, R.P. Ellwood d

Original Paper. D.F. Côrtes a K.R. Ekstrand b A.R. Elias-Boneta c, R.P. Ellwood d Original Paper Caries Res 2000;34:443 447 Received: December 9, 1999 Accepted after revision: July 10, 2000 An in vitro Comparison of the Ability of Fibre-Optic Transillumination, Visual Inspection and

More information

Caries Detection Technology

Caries Detection Technology Caries Technology Early caries detection is essential in maintaining oral health; luckily, many products on the market help dentists do so in a particularly easy fashion. Dentaltown s look at some of the

More information

Title. Citation 北海道歯学雑誌, 38(Special issue): Issue Date Doc URL. Type. File Information.

Title. Citation 北海道歯学雑誌, 38(Special issue): Issue Date Doc URL. Type. File Information. Title The ICDAS (International Caries Detection & Assessme Author(s)Kanehira, Takashi; Takehara, Junji; Nakamura, Kimiya Citation 北海道歯学雑誌, 38(Special issue): 180-183 Issue Date 2017-09 Doc URL http://hdl.handle.net/2115/67359

More information

Images that change your world.

Images that change your world. Images that change your world. NEW a whole new perspective on caries A whole new perspective on caries Optimum diagnosis thanks to top quality images. Significantly improved diagnosis quality in unsurpassed

More information

DENTAL CLINICS OF NORTH AMERICA. Emmiam. Incipient and Hidden Caries. GUEST EDITOR Daniel W. Boston, DMD. October 2005 Volume49 Number4

DENTAL CLINICS OF NORTH AMERICA. Emmiam. Incipient and Hidden Caries. GUEST EDITOR Daniel W. Boston, DMD. October 2005 Volume49 Number4 DENTAL CLINICS OF NORTH AMERICA Incipient and Hidden Caries GUEST EDITOR Daniel W. Boston, DMD October 2005 Volume49 Number4 Emmiam An Imprint of Elsevier, Inc. PHILADELPHIA LONDON TORONTO MONTREAL SYDNEY

More information

Scanning Is Believing

Scanning Is Believing KaVo DIAGNOdent /DIAGNOdent pen Laser Caries Detection Aid Scanning Is Believing Reveal the Hidden... Treat with Confidence NEW DIAGNOdent pen! Why you need DIAGNOdent Why yyou need If you cannot detect

More information

An in vitro experiment in the early diagnosis of caries using four diagnostic methods

An in vitro experiment in the early diagnosis of caries using four diagnostic methods An in vitro experiment in the early diagnosis of caries using four diagnostic methods M. Peneva 1, Rashkova 2, M., Golchev Y 3 Sofia, Bulgaria Summary The visual diagnostics of early caries is an extremely

More information

Current Concepts in Caries Management Diagnostic, Treatment and Ethical/Medico-Legal Considerations. Radiographic Caries Diagnosis

Current Concepts in Caries Management Diagnostic, Treatment and Ethical/Medico-Legal Considerations. Radiographic Caries Diagnosis Current Concepts in Caries Management Diagnostic, Treatment and Ethical/Medico-Legal Considerations Richard N. Bohay, DMD, MSc, MRCDC Associate Professor, Schulich School of Medicine & Dentistry Schulich

More information

Caries prevention is critical for children, especially. Quantitative Assessment of Enamel Hypomineralization. Permanent Molars of Children in China

Caries prevention is critical for children, especially. Quantitative Assessment of Enamel Hypomineralization. Permanent Molars of Children in China Scientific Article Quantitative Assessment of Enamel Hypomineralization by KaVo DIAGNOdent at Different Sites on First Permanent Molars of Children in China Shao-Min Li, DDS Jing Zou, DDS, MS Zhengyan

More information

Chapter 5. Investigation of in vitro Dental Erosion by Optical Techniques

Chapter 5. Investigation of in vitro Dental Erosion by Optical Techniques Chapter 5 Investigation of in vitro Dental Erosion by Optical Techniques 5.1 INTRODUCTION 5 Dental erosion is becoming an increasing predicament in dentistry and can be attributed to either exogenous

More information

CS Introducing the CS 1600 Intraoral Camera Revolutionary technology. Superior workflow.

CS Introducing the CS 1600 Intraoral Camera Revolutionary technology. Superior workflow. CS 1600 Introducing the CS 1600 Intraoral Camera Revolutionary technology. Superior workflow. Early caries identfication for healthier patients Earliest caries identification Early detection is the most

More information

The Efficacy of Laser Fluorescence to Detect in Vitro Demineralization and Remineralization. of smooth enamel surfaces.

The Efficacy of Laser Fluorescence to Detect in Vitro Demineralization and Remineralization. of smooth enamel surfaces. Photomedicine and Laser Surgery Volume 27, Number 1, 2009 Mary Ann Liebert, Inc. Pp. 57 61 DOI: 10.1089/pho.2007.2230 The Efficacy of Laser Fluorescence to Detect in Vitro Demineralization and Remineralization

More information

In Vitro Evaluation of the Spectra Early Caries Detection System

In Vitro Evaluation of the Spectra Early Caries Detection System In Vitro Evaluation of the Spectra Early Caries Detection System Maria Graye, DMD Pedodontist in Private Practice Hamilton, NJ, USA Kenneth Markowitz, DDS, MSD Assistant Professor, Department of Oral Biology

More information

Prevalence of White Spot Lesions during Orthodontic Treatment

Prevalence of White Spot Lesions during Orthodontic Treatment Virginia Commonwealth University VCU Scholars Compass Theses and Dissertations Graduate School 2009 Prevalence of White Spot Lesions during Orthodontic Treatment Julian Dixon Virginia Commonwealth University

More information

Title remineralization therapy. Issue Date Australian Dental Associatio

Title remineralization therapy. Issue Date Australian Dental Associatio NAOSITE: Nagasaki University's Ac Title Author(s) Early detection of white spot lesio remineralization therapy. Iijima, Youichi Citation Australian dental journal, 53(3), p Issue Date 2008-09 URL http://hdl.handle.net/10069/23123

More information

Diagnostic Values of Laser Fluorescence Device Compared to Other Techniques in Occlusal Caries Detection

Diagnostic Values of Laser Fluorescence Device Compared to Other Techniques in Occlusal Caries Detection Original Article Diagnostic Values of Laser Fluorescence Device Compared to Other Techniques in Occlusal Caries Detection M. Biria 1, 2, M. Moshfeghi 3, A. Akbarzadeh Baghban 4, A. Chehresaz 5 1 Assistant

More information

PDF hosted at the Radboud Repository of the Radboud University Nijmegen

PDF hosted at the Radboud Repository of the Radboud University Nijmegen PDF hosted at the Radboud Repository of the Radboud University Nijmegen The following full text is a publisher's version. For additional information about this publication click this link. http://hdl.handle.net/2066/71282

More information

MinimalInvasiveTechniquesinCariesDetectionDiagnosisandMangagementAClinicalStudy

MinimalInvasiveTechniquesinCariesDetectionDiagnosisandMangagementAClinicalStudy Global Journal of Medical Research: J Dentistry & Otolaryngology Volume 16 Issue 2 Version 1.0 Year 2016 Type: Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc.

More information

We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors

We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 3,800 116,000 120M Open access books available International authors and editors Downloads Our

More information

NON-IONIZING DIAGNOSTIC PROCEDURES

NON-IONIZING DIAGNOSTIC PROCEDURES NON-IONIZING DIAGNOSTIC PROCEDURES UnitedHealthcare Dental Clinical Policy Policy Number: DCP041.01 Effective Date: February 1, 2017 Table of Contents Page INSTRUCTIONS FOR USE...1 BENEFIT CONSIDERATIONS...1

More information

Photoluminescence spectra of sound tooth and those of different carious stages

Photoluminescence spectra of sound tooth and those of different carious stages Journal Biological Series 7(1): 000-000, January 2019 DOI: 10.15413/jbs2019.0121 2019 Academia Publishing Research Paper Photoluminescence spectra of sound tooth and those of different carious stages Accepted

More information

Emphasis has been placed on the diagnosis of occlusal

Emphasis has been placed on the diagnosis of occlusal Scientific Article Occlusal Caries Detection: A Comparison of a Laser Fluorescence System and Conventional Methods Cíntia Burin, DDS 1 Cristian Burin, DDS 1 Alessandro Dourado Loguercio, DDS, MS, PhD 2

More information

Bacterial Plaque and Its Relation to Dental Diseases. As a hygienist it is important to stress the importance of good oral hygiene and

Bacterial Plaque and Its Relation to Dental Diseases. As a hygienist it is important to stress the importance of good oral hygiene and Melissa Rudzinski Preventive Dentistry Shaunda Clark November 2013 Bacterial Plaque and Its Relation to Dental Diseases As a hygienist it is important to stress the importance of good oral hygiene and

More information

Investigation of Dental Caries Using Laser and Light-Induced Autofluorescence Methods

Investigation of Dental Caries Using Laser and Light-Induced Autofluorescence Methods Bulg. J. Phys. 33 (2006) 55 67 Investigation of Dental Caries Using Laser and Light-Induced Autofluorescence Methods E. Borisova 1, Tz. Uzunov 2, L. Avramov 1 1 Institute of Electronics, Bulgarian Academy

More information

Update in Caries Diagnosis, Management, and Prevention

Update in Caries Diagnosis, Management, and Prevention 2008 1 Update in Caries Diagnosis, Management, and Prevention Jenny Dennings, Veronika Stiles, Sarah Thornley, Katherine Yee University of Michigan E-Learning Program HYGDCE 482 001 W08 April 15, 2008

More information

SHOFU BLOCK & DISK CAD/CAM CERAMIC-BASED RESTORATIVE. Visit or call mm x 12mm x 16mm. 98mm x 14mm. 12mm x 14mm x 18mm

SHOFU BLOCK & DISK CAD/CAM CERAMIC-BASED RESTORATIVE. Visit   or call mm x 12mm x 16mm. 98mm x 14mm. 12mm x 14mm x 18mm SHOFU BLOCK & DISK CAD/CAM CERAMIC-BASED RESTORATIVE 1mm x 12mm x 16mm 12mm x 14mm x 18mm 98mm x 14mm Visit www.shofu.com or call 8.827.4638 Created through a rigorous manufacturing process, Shofu Blocks

More information

Update in Caries Diagnosis

Update in Caries Diagnosis Update in Caries Diagnosis Jenny Smits, AAS, RDH University of Michigan E-Learning Program 482 Oral Diseases: Prevention & Management University of Michigan Dental Desktop Support 12/16/2009 Introduction

More information

71 Photothermal Radiometry and Modulated Luminescence: Applications for Dental Caries Detection

71 Photothermal Radiometry and Modulated Luminescence: Applications for Dental Caries Detection j1047 71 Photothermal Radiometry and Modulated Luminescence: Applications for Dental Caries Detection Jose A. Garcia, Andreas Mandelis, Stephen H. Abrams, and Anna Matvienko 71.1 Introduction The traditional

More information

Original Article INTRODUCTION. Nandikolla Sagarika, Sundaramoorthy Suchindran 1, SC Loganathan, Velayutham Gopikrishna. Abstract

Original Article INTRODUCTION. Nandikolla Sagarika, Sundaramoorthy Suchindran 1, SC Loganathan, Velayutham Gopikrishna. Abstract Original Article Prevalence of white spot lesion in a section of Indian population undergoing fixed orthodontic treatment: An in vivo assessment using the visual International Caries Detection and Assessment

More information

SHOFU BLOCK & DISK CAD/CAM CERAMIC-BASED RESTORATIVE. Visit or call mm x 14mm

SHOFU BLOCK & DISK CAD/CAM CERAMIC-BASED RESTORATIVE. Visit   or call mm x 14mm SHOFU BLOCK & DISK CAD/CAM CERAMIC-BASED RESTORATIVE 12mm x 14mm x 18mm 98mm x 14mm Visit www.shofu.com or call 8.827.4638 Created through a rigorous manufacturing process, Shofu Blocks & Disks HC demonstrate

More information

Early lesion detection at 6-7 years and years schoolchildren from Bucharest, a prediction factor of decay evolution

Early lesion detection at 6-7 years and years schoolchildren from Bucharest, a prediction factor of decay evolution Early lesion detection at 6-7 years and 12-13 years schoolchildren from Bucharest, a prediction factor of decay evolution Roxana Ranga 1, Adina Dumitrache 2, Oana Slusanschi 3, Marian Cuculescu 4 Bucharest,

More information

Lec. 3-4 Dr. Saif Alarab Clinical Technique for Class I Amalgam Restorations The outline form

Lec. 3-4 Dr. Saif Alarab Clinical Technique for Class I Amalgam Restorations The outline form Lec. 3-4 Dr. Saif Alarab Clinical Technique for Class I Amalgam Restorations Class I refers to -Restorations on the occlusal surfaces of posterior teeth, - The occlusal two thirds of facial and lingual

More information

Treatment of Post-Orthodontic whitespot Lesions By Resin Infiltration

Treatment of Post-Orthodontic whitespot Lesions By Resin Infiltration IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-issn: 2279-0853, p-issn: 2279-0861.Volume 16, Issue 11 Ver. I (Nov. 2017), PP 80-84 www.iosrjournals.org Treatment of Post-Orthodontic whitespot

More information

Direct composite restorations for large posterior cavities extended range of applications for high-performance materials

Direct composite restorations for large posterior cavities extended range of applications for high-performance materials Direct composite restorations for large posterior cavities extended range of applications for high-performance materials A case study by Ann-Christin Meier, Dr. med. dent., Stapelfeld, Germany When large

More information

Electronic Dental Records

Electronic Dental Records Electronic Dental Records Dr. Douglas K Benn, Professor of Maxillofacial Radiology & Director of Oral Diagnostic Systems, University of Florida and Health Conundrums LLC 8/2/2008 Dr Benn, University of

More information

Diagnosis versus Detection of Caries

Diagnosis versus Detection of Caries Caries Res 2004;38:192 198 DOI: 10.1159/000077754 Diagnosis versus Detection of Caries B. Nyvad School of Dentistry, Faculty of Health Sciences, University of Aarhus, Aarhus, Denmark Key Words Dental caries,

More information

The 4Cs in Solving the Caries Puzzle

The 4Cs in Solving the Caries Puzzle The 4Cs in Solving the Caries Puzzle CAMBRA Caries Detection & Assesment System (ICDAS) Case-based protocol Calcium/Phosphate & Fluoride Objectives: Engaging in this program, the participant will be able

More information

SmartCrown. The Cavity Fighting SmartCrown. Patient Education Booklet. SmartCrown.com Toll Free Local

SmartCrown. The Cavity Fighting SmartCrown. Patient Education Booklet. SmartCrown.com Toll Free Local The Cavity Fighting SmartCrown SmartCrown Ca2+ SmartCrown.com Patient Education Booklet www.smartcrown.com support@smartcrown.com 800.255.2839 Toll Free 801.281.4012 Local 801.281.4022 Fax F- PO43- Origin

More information

ECC II Caries Disease Status. Drs Francisco Ramos-Gomez, Man Wai Ng and Jessica Lee

ECC II Caries Disease Status. Drs Francisco Ramos-Gomez, Man Wai Ng and Jessica Lee ECC II Caries Disease Status Drs Francisco Ramos-Gomez, Man Wai Ng and Jessica Lee Strategies for the detection and monitoring of early carious lesions. Detection and Staging Traditional Non- Surgical

More information

Remineralization Assessment of Early Childhood Caries using QLF-D: A Randomized Clinical Trial

Remineralization Assessment of Early Childhood Caries using QLF-D: A Randomized Clinical Trial ISSN (Print) : 0974-6846 ISSN (Online) : 0974-5645 Indian Journal of Science and Technology, Vol 9(40), DOI: 10.17485/ijst/2016/v9i40/103251, October 2016 Remineralization Assessment of Early Childhood

More information

Advanced Probing Techniques

Advanced Probing Techniques Module 21 Advanced Probing Techniques MODULE OVERVIEW The clinical periodontal assessment is one of the most important functions performed by dental hygienists. This module begins with a review of the

More information

EFFICACY OF LASER FLUORESCENCE IN DENTAL CARIES DIAGNOSIS: A META-ANALYSIS. Derek T. Fagen LCDR, DC, USN

EFFICACY OF LASER FLUORESCENCE IN DENTAL CARIES DIAGNOSIS: A META-ANALYSIS. Derek T. Fagen LCDR, DC, USN EFFICACY OF LASER FLUORESCENCE IN DENTAL CARIES DIAGNOSIS: A META-ANALYSIS by Derek T. Fagen LCDR, DC, USN A thesis submitted to the Faculty of the Comprehensive Dentistry Graduate Program Naval Postgraduate

More information

INTERCHANGEABLE HEAD SYSTEM. Easily and quickly change between caries diagnosis and intraoral imaging. T r i t o n H D

INTERCHANGEABLE HEAD SYSTEM. Easily and quickly change between caries diagnosis and intraoral imaging. T r i t o n H D INTERCHANGEABLE HEAD SYSTEM. Easily and quickly change between caries diagnosis and intraoral imaging. T r i t o n H D Whether its patient education, caries detection or accurate clinical documentation,

More information

PREVALENCE OF WHITE SPOT LESIONS DURING THE PROCEDURE OF FIXED ORTHODONTIC TREATMENT

PREVALENCE OF WHITE SPOT LESIONS DURING THE PROCEDURE OF FIXED ORTHODONTIC TREATMENT Original Article Received : 18 04 15 Review completed : 24 05 15 Accepted : 30 05 15 PREVALENCE OF WHITE SPOT LESIONS DURING THE PROCEDURE OF FIXED ORTHODONTIC TREATMENT Saibel Farishta, * Saurabh Sharma,

More information

Practice Impact Questionnaire

Practice Impact Questionnaire Practice Impact Questionnaire Your practitioner identifier is: XXXXXXXX It is very important that ONLY YOU complete this questionnaire because your responses will be compared to responses that you provided

More information

BASCD Trainers Pack for Caries Prevalence Studies. Updated: June 2014 for UK Training & Calibration exercise for the Deciduous Dentition

BASCD Trainers Pack for Caries Prevalence Studies. Updated: June 2014 for UK Training & Calibration exercise for the Deciduous Dentition BASCD Trainers Pack for Caries Prevalence Studies Updated: June 2014 for UK Training & Calibration exercise for the Deciduous Dentition Prepared by Helen Paisley, Cynthia Pine and Girvan Burnside Administrative

More information

Multi center study evaluating safety and effectiveness of The Canary System : An Interim Analysis

Multi center study evaluating safety and effectiveness of The Canary System : An Interim Analysis Multi center study evaluating safety and effectiveness of The Canary System : An Interim Analysis S.H. Abrams a, K. Sivagurunathan a,r. J. Jeon a, J.D. Silvertown a, A. Hellen a, A. Mandelis a, W.M.P.

More information

Contemporary Policy Implications to Control and Prevent Dental Caries. Policies are formed to achieve outcomes? Are outcomes being achieved?

Contemporary Policy Implications to Control and Prevent Dental Caries. Policies are formed to achieve outcomes? Are outcomes being achieved? Contemporary Policy Implications to Control and Prevent Dental Caries Policies are formed to achieve outcomes? Are outcomes being achieved? 2 3 4 Temple University School of Dentistry s Mission is the

More information

EFFECT OF ACIDULATED PHOSPHATE FLUORIDE GEL ON THE PREVENTION OF WHITE SPOT LESIONS IN PATIENTS UNDERGOING ACTIVE ORTHODONTIC TREATMENT.

EFFECT OF ACIDULATED PHOSPHATE FLUORIDE GEL ON THE PREVENTION OF WHITE SPOT LESIONS IN PATIENTS UNDERGOING ACTIVE ORTHODONTIC TREATMENT. EFFECT OF ACIDULATED PHOSPHATE FLUORIDE GEL ON THE PREVENTION OF WHITE SPOT LESIONS IN PATIENTS UNDERGOING ACTIVE ORTHODONTIC TREATMENT A Thesis by CORNEIL KIMBERLY HUTTO FRETTY Submitted to the Office

More information

VistaCam ix HD for diagnostics support in HD quality

VistaCam ix HD for diagnostics support in HD quality VistaCam ix HD for diagnostics support in HD quality The innovative intraoral camera with interchangeable head system COMPRESSED AIR SUCTION IMAGING DENTAL CARE HYGIENE Vista Cam ix HD real HD resolution

More information

Patient had no significant findings in medical history. Her vital signs were 130/99, pulse 93.

Patient had no significant findings in medical history. Her vital signs were 130/99, pulse 93. Julia Collins Den 1200 Journal #4 1. Demographics Patient is J.S. age 29, Heavy/II 2. Assessment Patient had no significant findings in medical history. Her vital signs were 130/99, pulse 93. Patient does

More information

أ.م. هدى عباس عبد اهلل CROWN AND BRIDGE جامعة تكريت كلية. Lec. (2) طب االسنان

أ.م. هدى عباس عبد اهلل CROWN AND BRIDGE جامعة تكريت كلية. Lec. (2) طب االسنان Lec. (2) CROWN AND BRIDGE أ.م. هدى عباس عبد اهلل Patient selection and examination A thorough diagnosis must first be made of the patient's dental condition, considering both hard and soft tissues. this

More information

A COMPARISON OF THE CLINICAL AXIAL EXTENSION OF CLASS II CARIOUS LESIONS WITH DIFFERENT DIAGNOSTIC IMAGES

A COMPARISON OF THE CLINICAL AXIAL EXTENSION OF CLASS II CARIOUS LESIONS WITH DIFFERENT DIAGNOSTIC IMAGES A COMPARISON OF THE CLINICAL AXIAL EXTENSION OF CLASS II CARIOUS LESIONS WITH DIFFERENT DIAGNOSTIC IMAGES Mohammed S. H. Bin-Shuwaish, B.D.S. A thesis submitted in partial fulfillment of the requirements

More information

Correlation between Laser Fluorescence Readings and Volume of Tooth Preparation in Incipient Occlusal Caries In Vitrojerd_

Correlation between Laser Fluorescence Readings and Volume of Tooth Preparation in Incipient Occlusal Caries In Vitrojerd_ Correlation between Laser Fluorescence Readings and Volume of Tooth Preparation in Incipient Occlusal Caries In Vitrojerd_309 31..39 EDUARDO S. GHANAME, DDS, MS* ANDRÉ V. RITTER, DDS, MS HARALD O. HEYMANN,

More information

COVER FEATURE SYNOPSIS

COVER FEATURE SYNOPSIS Supplementary Methods for Detection and Quantification of Dental Caries Lena Karlsson, RDH; Sofia Tranæus, DDS, PhD Department of Cariology and Endodontology, Institute of Odontology, Karolinska Institute,

More information

Influence of education level and experience on detection of approximal caries in digital dental radiographs. An in vitro study

Influence of education level and experience on detection of approximal caries in digital dental radiographs. An in vitro study swed dent j 2010; 34: 63-69 hellén-halme, hänsel petersson Influence of education level and experience on detection of approximal caries in digital dental radiographs. An in vitro study Kristina Hellén-Halme

More information

Recovery percentage of remineralization according to severity of early caries

Recovery percentage of remineralization according to severity of early caries Research Article Recovery percentage of remineralization according to severity of early caries HEE EUN KIM, RDH, PHD, HO KEUN KWON, DDS, PHD & BAEK IL KIM, DDS, PHD ABSTRACT: Purpose: To analyze the cutoff

More information

The Cavity Fighting ProActive Crown

The Cavity Fighting ProActive Crown The Cavity Fighting ProActive Crown Powered by Ketac Nano Patient Education Booklet www.proactivedentist.com support@proactivedentist.com 800.255.2839 Toll Free 801.281.4012 Local 801.281.4022 Fax Origin

More information

Essentials of. Dental Assisting. Edition 6. Debbie S. Robinson Doni L. Bird

Essentials of. Dental Assisting. Edition 6. Debbie S. Robinson Doni L. Bird Essentials of Dental Assisting Edition 6 Debbie S. Robinson Doni L. Bird CHAPTER21 Restorative Procedures http://evolve.elsevier.com/robinson/essentials/ LEARNING OBJECTIVES KEY TERMS 1. Pronounce, define,

More information

Continually Fluoride Releasing Aesthetic Dental Restorative Material

Continually Fluoride Releasing Aesthetic Dental Restorative Material Continually Fluoride Releasing Aesthetic Dental Restorative Material Research is our best product Image provided by Dr. Sushil Koirala BEAUTIFIL II More than just filling BEAUTIFIL II stands out for its

More information

Linking Research to Clinical Practice

Linking Research to Clinical Practice Prevention of Root Caries Denise M. Bowen, RDH, MS Linking Research to Clinical Practice The purpose of Linking Research to Clinical Practice is to present evidence based information to clinical dental

More information

Effect of Drying on the Reproducibility of DIAGNOdent to Detect Caries-Like Lesions

Effect of Drying on the Reproducibility of DIAGNOdent to Detect Caries-Like Lesions Braz Dent J (2010) 21(5): 405-410 Laser diagnosis of caries-like lesions ISSN 0103-6440 Effect of Drying on the Reproducibility of DIAGNOdent to Detect Caries-Like Lesions Camila PINELLI 1 Leonor de Castro

More information

Collaborators. Dental caries. Fluorescence and photochemical reactions: Lessons from teeth and tissues. 54,000+ patient appointments per year

Collaborators. Dental caries. Fluorescence and photochemical reactions: Lessons from teeth and tissues. 54,000+ patient appointments per year Fluorescence and photochemical reactions: Lessons from teeth and tissues Laurence J. Walsh School of Dentistry, The University of Queensland 2006 Collaborators Dr Jelena Vlacic Dr Fardad Shakabaie Dr Srdjan

More information

Restorative Case Presentation. Sharon Irwin

Restorative Case Presentation. Sharon Irwin Restorative Case Presentation Sharon Irwin Patient Details Male aged 13 DOB 28/07/00 Social History lives with Mother and siblings, 2 nd year high school Medical History fit and well Medication none Dental

More information

Minimally Invasive Dentistry

Minimally Invasive Dentistry Minimally Invasive Dentistry Full Summary Minimally Invasive Dentistry Defined: Minimally invasive dentistry (MID) is an evidence based intervention approach supported internationally that aims to do the

More information

Review Article Caries Detection Methods Based on Changes in Optical Properties between Healthy and Carious Tissue

Review Article Caries Detection Methods Based on Changes in Optical Properties between Healthy and Carious Tissue International Dentistry Volume 2010, Article ID 270729, 9 pages doi:10.1155/2010/270729 Review Article Caries Detection Methods Based on Changes in Optical Properties between Healthy and Carious Tissue

More information

Chapter 15 Detection of Early Caries by Laser-Induced Breakdown Spectroscopy

Chapter 15 Detection of Early Caries by Laser-Induced Breakdown Spectroscopy Chapter 15 Detection of Early Caries by Laser-Induced Breakdown Spectroscopy Yuji Matsuura Abstract To improve sensitivity of dental caries detection by laser-induced breakdown spectroscopy (LIBS) analysis,

More information

Indications The selection of amalgam as a restorative material for class V cavity should involve the following considerations:

Indications The selection of amalgam as a restorative material for class V cavity should involve the following considerations: 1 Lec.7 د.عبد املنعم اخلفاجي CLASS V CAVITY PREPARATION FOR AMAGLAM Indications The selection of amalgam as a restorative material for class V cavity should involve the following considerations: 1- Caries:

More information

PREDICTING IMPROVEMENT OF POST-ORTHODONTIC WHITE SPOT LESIONS

PREDICTING IMPROVEMENT OF POST-ORTHODONTIC WHITE SPOT LESIONS PREDICTING IMPROVEMENT OF POST-ORTHODONTIC WHITE SPOT LESIONS SUSAN KIM A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN DENTISTRY UNIVERSITY OF WASHINGTON

More information

Dental Depth Profilometric Diagnosis of Pit & Fissure Caries Using Frequency-Domain Infrared Photothermal Radiometry and Modulated Laser Luminescence

Dental Depth Profilometric Diagnosis of Pit & Fissure Caries Using Frequency-Domain Infrared Photothermal Radiometry and Modulated Laser Luminescence Dental Depth Profilometric Diagnosis of Pit & Fissure Caries Using Frequency-Domain Infrared Photothermal Radiometry and Modulated Laser Luminescence Raymond J. Jeon, Calvin Han, Andreas Mandelis* and

More information

2. Gap closure and replacement of the missing tooth 35 with directly modelled bridge region 34-36

2. Gap closure and replacement of the missing tooth 35 with directly modelled bridge region 34-36 GrandTEC Test Kit Dear User, This Test Kit has been put together to enable you to test GrandTEC on the model before using it in a clinical situation. GrandTEC is a resin-impregnated glass fibre strip.

More information

A novel quantitative light induced fluorescence device for monitoring molar incisor hypomineralization

A novel quantitative light induced fluorescence device for monitoring molar incisor hypomineralization Original Article A novel quantitative light induced fluorescence device for monitoring molar incisor hypomineralization B Durmus, A Durhan, B Gökkaya, B Kıtıki, F Yanıkoğlu, B Kargül Department of Pediatric

More information

SUBCHAPTER 16H - DENTAL ASSISTANTS SECTION CLASSIFICATION AND TRAINING

SUBCHAPTER 16H - DENTAL ASSISTANTS SECTION CLASSIFICATION AND TRAINING SUBCHAPTER 16H - DENTAL ASSISTANTS SECTION.0100 - CLASSIFICATION AND TRAINING 21 NCAC 16H.0101 CLASSIFICATION Based upon education, training, and experience, a dental assistant shall be categorized as

More information

Diagnosis of Pit and Fissure Caries Using Frequency-Domain Infrared Photothermal Radiometry and Modulated Laser Luminescence

Diagnosis of Pit and Fissure Caries Using Frequency-Domain Infrared Photothermal Radiometry and Modulated Laser Luminescence Original Paper DOI: 10.1159/000080579 Received: August 1, 2003 Accepted after revision: February 24, 2004 Diagnosis of Pit and Fissure Caries Using Frequency-Domain Infrared Photothermal Radiometry and

More information

VistaCam ix Proof interchangeable head

VistaCam ix Proof interchangeable head VistaCam ix Proof interchangeable head Installation and Operating Instructions 9000-618-178/30 2014/07 Content Important information 1. About this document 4 1.1 Warnings and Symbols 4 1.2 Copyright notice

More information

CLASS II AMALGAM RESTORATIONS. Amalgam restorations that restore one or both of the proximal surfaces of the tooth

CLASS II AMALGAM RESTORATIONS. Amalgam restorations that restore one or both of the proximal surfaces of the tooth CLASS II AMALGAM RESTORATIONS Amalgam restorations that restore one or both of the proximal surfaces of the tooth Initial Tooth Preparation Occlusal outline form (occlusal step).the occlusal outline form

More information

Detection of initial caries lesions on smooth surfaces by quantitative light-induced fluorescence and visual examination: an in vivo comparison

Detection of initial caries lesions on smooth surfaces by quantitative light-induced fluorescence and visual examination: an in vivo comparison Eur J Oral Sci 2005; 113: 494 498 Printed in Singapore. All rights reserved Copyright Ó Eur J Oral Sci 2005 European Journal of Oral Sciences Detection of initial caries lesions on smooth surfaces by quantitative

More information

FACT SHEET: ABOUT DENTAL CARIES

FACT SHEET: ABOUT DENTAL CARIES FACT SHEET: ABOUT DENTAL CARIES What is dental caries? Dental caries is the technical term used by healthcare and dental professionals to describe the disease more commonly known as tooth decay. 1-2 Dental

More information

An in vitro comparison of quantitative light-induced fluorescence-digital and spectrophotometer on monitoring artificial white spot lesions

An in vitro comparison of quantitative light-induced fluorescence-digital and spectrophotometer on monitoring artificial white spot lesions Photodiagnosis and Photodynamic Therapy (2015) 12, 378 384 Available online at www.sciencedirect.com ScienceDirect journal homepage: www.elsevier.com/locate/pdpdt An in vitro comparison of quantitative

More information

Remineralization of early caries by chewing sugar-free gum: A clinical study using quantitative light-induced fluorescence

Remineralization of early caries by chewing sugar-free gum: A clinical study using quantitative light-induced fluorescence Research Article Remineralization of early caries by chewing sugar-free gum: A clinical study using quantitative light-induced fluorescence YING DONG, MDS, WEI YIN, DDS, PHD, DEYU HU, DDS, PHD, XIMU ZHANG,

More information

Preparation and making fillings Class V., III., IV.

Preparation and making fillings Class V., III., IV. Preparation and making fillings Class V., III., IV. Class V. Cervical defects - Dental caries - Non carious lesions (erosion, abrasion, V shaped defects) Types of defects Caries Erosion Abrasion V shaped

More information

Measurement of surface hardness of primary carious lesions in extracted human enamel Measurement of Knoop hardness using Cariotester

Measurement of surface hardness of primary carious lesions in extracted human enamel Measurement of Knoop hardness using Cariotester Dental Materials Journal 2015; 34(2): 252 256 Measurement of surface hardness of primary carious lesions in extracted human enamel Measurement of Knoop hardness using Cariotester Akihiko SHIMIZU 1, Takatsugu

More information