Ethanol-based EDC solution preparation -

Transcription

1 Am J Dent Ethanol-based EDC solution preparation - Specimen preparation -

2 Microtensile bond strength (TBS) test - Scanning electron microscopy (SEM) observation - Dentin surface treatment & bonding Resistance against enzymatic degradation test -

3 Zhang et al Microtensile bond strength testing - SEM of bonding interfaces - Amount of hydroxyproline release -

4 Dentin surface treatment & bonding

5 212 Zhang et al and 1 M ethanol-based EDC groups than in the untreated group, suggesting that using either 0.3 M or 1.0 M ethanol-based EDC solution for dentin surface pretreatment before bonding procedure can significantly increase the resistance of dentin collagen to biodegradation within a clinically relevant time. Within the limitations of this in vitro study, the results suggest that ethanol-based EDC surface treatment for 60 seconds preserves the resin-dentin bond strength and increases the resistance of dentin collagen to biodegradation. The 0.3 M ethanolbased EDC solution may be an optimal concentration for dentin surface treatment. Further studies are recommended to validate the feasibility of incorporating ethanol-based EDC as a component in etch-and-rinse or self-etch adhesives in order to support its in vivo application. a. Ekar, Shanghai, China. b. Sinopharm Chemical Reagent Co., Ltd, Beijing, China. c. Buehler, Lake Bluff, IL, USA. d. Heraeus-Kulzer Inc, Hanau, Germany. e. 3M ESPE, St. Paul, MN, USA. f. Mitutoyo Co., Ltd, Kanagawa, Japan. g. Henkel Adhesives, Shantou, China. h. Shimadzu Co., Ltd, Kyoto, Japan. i. Tongfang, Shanghai, China. j. SPSS Inc, Chicago, IL, USA. k. Shumei, Kunshan, China. l. Leica Microsystems Inc, Buffalo Grove, IL, USA. m. Hitachi, Tokyo, Japan. n. Sigma, St Louis, MO, USA. o. Eppendorf, Hamburg, Germany. p. R&D Systems, Minneapolis, MN, USA. q. PerkinElmer, Seer Green, UK. Disclosure statement: The authors declared no conflict of interest. Dr. Zhang is a Research Fellow, Dr. Tang is a predoctoral student, Dr. Liu is Professor, Dr. Zhou is Professor and Chair, Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China. Dr. Chung is Professor, Dr. Chan is Professor and Chair, Department of Restorative Dentistry, University of Washington, Seattle, Washington, USA. References 1. Dennison JB, Sarrett DC. 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