Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic
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1 Supplementary Information The title of the manuscript Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke Xin-Wei He 1, Wei-Ling Li 1, Cai Li 1, Peng Liu 1, Yu-Guang Shen 1, Min Zhu 2, Xiao-Ping Jin 1* 1 Department of Neurology, Taizhou Hospital, Wenzhou Medical University, Linhai, , China 2 Medical Research Center, Taizhou Hospital, Wenzhou Medical University, Linhai, , China * corresponding author, hhh @aliyun.com
2 Galectin-1 Galectin-3 Galectin-9 Galectin-3BP Galectin-1 / r c = a p = r = a p < r = a p = Galectin-3 r c = b p = / r = a p < r = p = Galectin-9 r = b p < r = b p = / r = a p = Galectin-3BP r = b p = r = b p = r = b p = / Supplemental Table S1. Correlations between serum levels of galectin-1, galectin-3, galectin-3bp, and galectin-9 a Correlation coefficient in controls b Correlation coefficient in patients r c represents Pearson's correlation coefficient r represents Spearman's rank correlation coefficient Abbreviations: Galectin-3BP, galectin-3-binding protein
3 Galectin-1 Galectin-3 Galectin-9 Galectin-3BP r p r p r p r p NIHSS on day NIHSS on day Stroke volume Supplementary Table S2. Correlation of serum levels of galectin-1, -3, -9, and -3BP with stroke severity. r represents Spearman's rank correlation coefficient. Abbreviations: Galectin-3BP, galectin-3-binding protein; NIHSS, National Institutes of Health Stroke Scale.
4 Characteristic Patients have the second blood samples All stroke patients (n = 130) p value (n = 109) Age (years) 70.1 ± ± Male (%) 71 (65.1) 79 (60.8) SBP (mmhg) ± ± DBP (mmhg) 80.9 ± ± BMI (kg/m 2 ) ± ± FBG (mmol/l) 5.37 (4.81, 6.35) 5.37 (4.82, 5.85) TG (mmol/l) 1.32 (1.03, 1.76) 1.31 (1.02, 1.72) TC (mmol/l) 4.59 ± ± HDL-C (mmol/l) 1.10 (1.00, 1.31) 1.12 (1.01, 1.36) LDL-C (mmol/l) 2.50 (2.17, 3.17) 2.50 (2.16, 3.20) HbA 1C (%) 5.8 (5.4, 6.2) 5.8 (5.5, 6.2) Homocysteine (μmol/l) 12.3 (10.6, 15.5) 13.1 (10.9, 15.9) Serum creatinine (μmol/l) 72.4 ± ± Hs-CRP (mg/l) 3.30 (2.08, 5.90) 3.05 (2.18, 5.80) Hypertension 86 (78.9) 101 (77.7) Diabetes mellitus 25 (22.9) 31 (23.8) Dyslipidaemia 46 (42.2) 54 (41.5) Smokers 48 (44.0) 52 (40.0) Alcohol consumers 23 (21.1) 26 (20.0) Hypertension med use 28 (32.6 a ) 33 (32.7 a ) Diabetes med use 14 (56.0 b ) 19 (61.3 b ) NIHSS on day 1 4 (2, 8) 4 (2, 8) mrs scores 3 (2, 4) 3 (2, 4) Supplementary Table S3. Baseline characteristics of the subgroup of patients have the second blood samples on day 6 after stroke and comparison to the whole LAA stroke patients. Continuous variables are expressed as the mean ± standard deviation (SD) or the median (interquartile range). Categorical values are presented as frequencies (percentages). a represents the percentage in the hypertension population. b represents the percentage in the diabetes population. Abbreviations: LAA stroke, large artery atherosclerotic stroke; SBP, systolic blood pressure; DBP, diastolic blood pressure; BMI, body mass index; FBG, fasting blood glucose; TG, triglycerides; TC, total cholesterol; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; HbA 1C, haemoglobin A1c; hs-crp, high-sensitivity C-reactive protein; NIHSS, National Institutes of
5 Health Stroke Scale; mrs, modified Rankin Scale. Characteristic Patients have the third blood samples Patients have the second blood samples p value (n = 39) (n = 109) Age (years) 69.6 ± ± Male (%) 25 (64.1) 71 (65.1) SBP (mmhg) ± ± DBP (mmhg) 82.2 ± ± BMI (kg/m 2 ) ± ± FBG (mmol/l) 5.47 (5.04, 7.46) 5.37 (4.81, 6.35) TG (mmol/l) 1.28 (1.09, 1.72) 1.32 (1.03, 1.76) TC (mmol/l) 4.93 ± ± HDL-C (mmol/l) 1.16 (1.04, 1.37) 1.10 (1.00, 1.31) LDL-C (mmol/l) 3.08 (2.49, 3.63) 2.50 (2.17, 3.17) HbA 1C (%) 5.9 (5.5, 6.8) 5.8 (5.4, 6.2) Homocysteine (μmol/l) 14.5 (11.6, 17.6) 12.3 (10.6, 15.5) Serum creatinine (μmol/l) 70.2 ± ± Hs-CRP (mg/l) 2.8 (2.13, 6.08) 3.30 (2.08, 5.90) Hypertension 31 (79.5) 86 (78.9) Diabetes mellitus 12 (30.8) 25 (22.9) Dyslipidaemia 19 (48.7) 46 (42.2) Smokers 13 (33.3) 48 (44.0) Alcohol consumers 9 (23.1) 23 (21.1) Hypertension med use 11 (35.5 a ) 28 (32.6 a ) Diabetes med use 5 (41.7 b ) 14 (56.0 b ) NIHSS on day 1 3 (2, 4) 4 (2, 8) mrs scores 3 (1, 3) 3 (2, 4) Supplementary Table S4. Baseline characteristics of the subgroup of patients have the second blood samples on day 6 after stroke and third blood samples in 4th week after stroke. Continuous variables are expressed as the mean ± standard deviation (SD) or the median (interquartile range). Categorical values are presented as frequencies (percentages). a represents the percentage in the hypertension population. b represents the percentage in the diabetes population. Abbreviations: LAA stroke, large artery atherosclerotic stroke; SBP, systolic blood pressure; DBP, diastolic blood pressure; BMI, body mass index; FBG, fasting blood glucose; TG, triglycerides; TC, total
6 cholesterol; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; HbA 1C, haemoglobin A1c; hs-crp, high-sensitivity C-reactive protein; NIHSS, National Institutes of Health Stroke Scale; mrs, modified Rankin Scale.
(n=6279). Continuous variables are reported as mean with 95% confidence interval and T1 T2 T3. Number of subjects
Table 1. Distribution of baseline characteristics across tertiles of OPG adjusted for age and sex (n=6279). Continuous variables are reported as mean with 95% confidence interval and categorical values
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