Short- and long-lasting consequences of in vivo nicotine treatment

Size: px
Start display at page:

Download "Short- and long-lasting consequences of in vivo nicotine treatment"

Transcription

1 Short- and long-lasting consequences of in vivo nicotine treatment on hippocampal excitability Rachel E. Penton, Michael W. Quick, Robin A. J. Lester Supplementary Figure 1. Histogram showing the maximal fepsp slope for different periods of withdrawal. 4-week (sal-slices, n = 12; nic-slices, n = 20), 3-month (sal-slices, n = 17; nic-slices, n = 22), 6-month (sal-slices, n = 6; nic-slices, n = 10), 9-month (salslices, n = 4; nic-slices, n = 5). Data are presented as the mean ± s.e.m.

2 Supplementary Figure 2. A, B, Mean miniature EPSP frequency (A) and amplitude (B) in CA1 pyramidal cells recorded in the presence of 1µM TTX from 1-day withdrawn animals (sal-cells, n = 4; nic-cells, n = 4). C, Timecourse of the resting membrane potential during TTX wash-in (sal-cells, n = 5; nic-cells, n = 4). Error bars are only shown once per minute for clarity. Data are presented as mean ± s.e.m. 2

3 Supplementary Figure 3. A, Mean current-voltage (I-V) curves for 500 ms hyperpolarizing and subthreshold depolarizing pulses in CA1 pyramidal cells from 1-day withdrawn animals in the presence of CNQX + D-APV (sal-cells, n = 5; nic-cells, n = 4). B, Mean current-voltage (I-V) curves for 500 ms hyperpolarizing and subthreshold depolarizing pulses in CA1 pyramidal cells from 1-day withdrawn animals in the presence of TTX (sal-cells, n = 4; nic-cells, n = 4). Data are presented as the mean ± s.e.m. 3

4 Supplementary Figure 4. A, Diagram of DG isolated slice. The scalpel cut is indicated. B, Mean frequencies of spontaneously occurring spikes recorded from DG stratum granulosum in both intact (sal-slices, n = 8; nic-slices, n = 7) and DG isolated (sal-slices, n = 8; nic-slices, n = 7) slices from 1-day withdrawn animals. Data are presented as the mean ± s.e.m. DG isolated, slices with connections severed between CA3 and dentate gyrus. 4

5 Supplementary Figure 5. A, Top: Examples of orthodromically evoked suprathreshold fepsps recorded in stratum radiatum (top traces) and pspikes recorded in stratum pyramidale (bottom traces) from 9-month withdrawn slices (sal-slices: red ; nic-slices: blue). Bottom: Examples of antidromically evoked aspikes recorded in stratum pyramidale (bottom traces) from 9-month withdrawn slices (sal-slices: red ; nic-slices: blue). Stimulus artifacts have been removed for clarity and black triangles represent time of stimulus. B, Mean extracellular E-S curves from long-term withdrawn (4-week + 9- month) no CA3 slices (sal-slices, n = 15; nic-slices, n = 19). C, Mean I-O curves for aspikes from long-term withdrawn (4-week + 9-month) no CA3 slices (sal-slices, n = 7; nic-slices, n = 7). Data are presented as the mean ± s.e.m. no CA3, slices with the CA3 region removed. 5

6 Supplementary Figure 6. A, Mean extracellular E-S curves from saline-treated 1-day withdrawn animals in no DG + no CA3 slices (n = 8) in ACSF (closed symbols) and in the presence of 500 nm nicotine (open symbols). B, Mean extracellular E-S curves from nicotine-treated 1-day withdrawn animals in no DG + no CA3 slices (n = 6) in ACSF (closed symbols) and in the presence of 500 nm nicotine (open symbols). Data are presented as the mean ± s.e.m. no DG, slices with connections severed between CA3 and dentate gyrus. no CA3, slices with the CA3 region removed. 6

7 Supplementary Table 1 Treatment Saline Nicotine Number of neurons RMP (mv) ± ± 0.79* R input (MΩ) ± ± 1.56 Sag ratio 0.84 ± ± 0.01 mahp (mv) ± ± 0.34 Action potential threshold (mv) ± ± 0.87 Action potential height (mv) ± ± 0.95 Action potential half-width (ms) 0.97 ± ± 0.01 fahp (mv) ± ± 0.91 Single-cell properties following 1-day of withdrawal from chronic nicotine or saline. Membrane properties and action potential characteristics measured by intracellular recordings from CA1 pyramidal cells in hippocampal slices prepared from saline- or nicotine-treated animals following 1-day of withdrawal. Values represent mean ± s.e.m. * P <

8 Supplementary Table 2 Treatment Saline Nicotine Number of neurons RMP (mv) ± ± 1.35 R input (MΩ) ± ± 2.70* Sag ratio 0.86 ± ± 0.01 mahp (mv) ± ± 0.62 Action potential threshold (mv) ± ± 1.21 Action potential height (mv) ± ± 1.49 Action potential half-width (ms) 1.00 ± ± 0.01 fahp (mv) ± ± 1.39 Single-cell properties following long-term withdrawal from chronic nicotine or saline in the presence of blockers of synaptic transmission. Membrane properties and action potential characteristics measured by intracellular recordings from CA1 pyramidal cells in the presence of blockers in hippocampal slices prepared from saline- or nicotine-treated animals following long-term withdrawal (4- week + 3-month). Values represent mean ± s.e.m. * P <

Short- and Long-Lasting Consequences of In Vivo Nicotine Treatment on Hippocampal Excitability

Short- and Long-Lasting Consequences of In Vivo Nicotine Treatment on Hippocampal Excitability 2584 The Journal of Neuroscience, February 16, 2011 31(7):2584 2594 Cellular/Molecular Short- and Long-Lasting Consequences of In Vivo Nicotine Treatment on Hippocampal Excitability Rachel E. Penton, 1

More information

Astrocyte signaling controls spike timing-dependent depression at neocortical synapses

Astrocyte signaling controls spike timing-dependent depression at neocortical synapses Supplementary Information Astrocyte signaling controls spike timing-dependent depression at neocortical synapses Rogier Min and Thomas Nevian Department of Physiology, University of Berne, Bern, Switzerland

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Supplementary Figure 1. Normal AMPAR-mediated fepsp input-output curve in CA3-Psen cdko mice. Input-output curves, which are plotted initial slopes of the evoked fepsp as function of the amplitude of the

More information

Title: Plasticity of intrinsic excitability in mature granule cells of the dentate gyrus

Title: Plasticity of intrinsic excitability in mature granule cells of the dentate gyrus Title: Plasticity of intrinsic excitability in mature granule cells of the dentate gyrus Authors: Jeffrey Lopez-Rojas a1, Martin Heine b1 and Michael R. Kreutz ac1 a Research Group Neuroplasticity, b Research

More information

Supplementary Figure 1. Basic properties of compound EPSPs at

Supplementary Figure 1. Basic properties of compound EPSPs at Supplementary Figure 1. Basic properties of compound EPSPs at hippocampal CA3 CA3 cell synapses. (a) EPSPs were evoked by extracellular stimulation of the recurrent collaterals and pharmacologically isolated

More information

Sample Lab Report 1 from 1. Measuring and Manipulating Passive Membrane Properties

Sample Lab Report 1 from  1. Measuring and Manipulating Passive Membrane Properties Sample Lab Report 1 from http://www.bio365l.net 1 Abstract Measuring and Manipulating Passive Membrane Properties Biological membranes exhibit the properties of capacitance and resistance, which allow

More information

Wenqin Hu, Cuiping Tian, Tun Li, Mingpo Yang, Han Hou & Yousheng Shu

Wenqin Hu, Cuiping Tian, Tun Li, Mingpo Yang, Han Hou & Yousheng Shu Distinct contributions of Na v 1.6 and Na v 1.2 in action potential initiation and backpropagation Wenqin Hu, Cuiping Tian, Tun Li, Mingpo Yang, Han Hou & Yousheng Shu Supplementary figure and legend Supplementary

More information

Enhancement of synaptic transmission by cyclic AMP modulation of presynaptic I h channels. Vahri Beaumont and Robert S. Zucker

Enhancement of synaptic transmission by cyclic AMP modulation of presynaptic I h channels. Vahri Beaumont and Robert S. Zucker Enhancement of synaptic transmission by cyclic AMP modulation of presynaptic I h channels Vahri Beaumont and Robert S. Zucker Background I h channels discovered in 1976 (Noma A. and Irisawa H.) Voltage-gated

More information

Supplementary Figure 1) GABAergic enhancement by leptin hyperpolarizes POMC neurons A) Representative recording samples showing the membrane

Supplementary Figure 1) GABAergic enhancement by leptin hyperpolarizes POMC neurons A) Representative recording samples showing the membrane Supplementary Figure 1) GABAergic enhancement by leptin hyperpolarizes POMC neurons A) Representative recording samples showing the membrane potential recorded from POMC neurons following treatment with

More information

Supplementary Figure 1. GABA depolarizes the majority of immature neurons in the

Supplementary Figure 1. GABA depolarizes the majority of immature neurons in the Supplementary Figure 1. GABA depolarizes the majority of immature neurons in the upper cortical layers at P3 4 in vivo. (a b) Cell-attached current-clamp recordings illustrate responses to puff-applied

More information

Ube3a is required for experience-dependent maturation of the neocortex

Ube3a is required for experience-dependent maturation of the neocortex Ube3a is required for experience-dependent maturation of the neocortex Koji Yashiro, Thorfinn T. Riday, Kathryn H. Condon, Adam C. Roberts, Danilo R. Bernardo, Rohit Prakash, Richard J. Weinberg, Michael

More information

SUPPLEMENTARY INFORMATION. Supplementary Figure 1

SUPPLEMENTARY INFORMATION. Supplementary Figure 1 SUPPLEMENTARY INFORMATION Supplementary Figure 1 The supralinear events evoked in CA3 pyramidal cells fulfill the criteria for NMDA spikes, exhibiting a threshold, sensitivity to NMDAR blockade, and all-or-none

More information

Supplementary Information

Supplementary Information Hyperpolarization-activated cation channels inhibit EPSPs by interactions with M-type K + channels Meena S. George, L.F. Abbott, Steven A. Siegelbaum Supplementary Information Part 1: Supplementary Figures

More information

File name: Supplementary Information Description: Supplementary Figures, Supplementary Table and Supplementary References

File name: Supplementary Information Description: Supplementary Figures, Supplementary Table and Supplementary References File name: Supplementary Information Description: Supplementary Figures, Supplementary Table and Supplementary References File name: Supplementary Data 1 Description: Summary datasheets showing the spatial

More information

1.0. FSL NMDAR-fEPSP 0.8. amplitude (mv) Intensity (µa) 2.0 SD FSL Time (ms)

1.0. FSL NMDAR-fEPSP 0.8. amplitude (mv) Intensity (µa) 2.0 SD FSL Time (ms) a 2.5 1. AMPAR-fEPSP slope (mv/ms) 2. 1. NMDAR-fEPSP amplitude (mv).8.6.4.5.2. 2 4 6 8. 1 2 3 4 5 Intensity (µa) Intensity (µa) b 2. PPF Ratio (fepsp2/fepsp1) 1..5. 5 1 2 5 Time (ms) Supplementary Figure

More information

Is action potential threshold lowest in the axon?

Is action potential threshold lowest in the axon? Supplementary information to: Is action potential threshold lowest in the axon? Maarten H. P. Kole & Greg J. Stuart Supplementary Fig. 1 Analysis of action potential (AP) threshold criteria. (a) Example

More information

Supporting Online Material for

Supporting Online Material for www.sciencemag.org/cgi/content/full/317/5841/183/dc1 Supporting Online Material for Astrocytes Potentiate Transmitter Release at Single Hippocampal Synapses Gertrudis Perea and Alfonso Araque* *To whom

More information

Contrasting Effects of the Persistent Na + Current on Neuronal Excitability and Spike Timing

Contrasting Effects of the Persistent Na + Current on Neuronal Excitability and Spike Timing Neuron 49, 257 270, January 19, 2006 ª2006 Elsevier Inc. DOI 10.1016/j.neuron.2005.12.022 Contrasting Effects of the Persistent Na + Current on Neuronal Excitability and Spike Timing Koen Vervaeke, 1,2,4

More information

Effects of uniform extracellular DC electric fields on excitability in rat hippocampal slices in vitro

Effects of uniform extracellular DC electric fields on excitability in rat hippocampal slices in vitro J Physiol 557.1 (2004) pp 175 190 175 Effects of uniform extracellular DC electric fields on excitability in rat hippocampal slices in vitro Marom Bikson 1, Masashi Inoue 2, Hiroki Akiyama 2, Jackie K.

More information

Is activation of N-methyl-D-aspartate receptor gated channels sufficient to induce long term potentiation?

Is activation of N-methyl-D-aspartate receptor gated channels sufficient to induce long term potentiation? Neuroscience Letters, 80 (1987) 283 288 283 Elsevier Scientific Publishers Ireland Ltd. NSL 04827 Is activation of N-methyl-D-aspartate receptor gated channels sufficient to induce long term potentiation?

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Arcuate ChIEF-tdTomato neurons expressed TH These micrographs show that TH-Cre-ChIEF-tdTomato (magenta), expressed by AAV in a TH-Cre mouse, were immunostained with TH (green) in

More information

Cellular/Molecular. The Journal of Neuroscience, February 21, (8):

Cellular/Molecular. The Journal of Neuroscience, February 21, (8): The Journal of Neuroscience, February 21, 2007 27(8):1853 1867 1853 Cellular/Molecular M-Channels (Kv7/KCNQ Channels) That Regulate Synaptic Integration, Excitability, and Spike Pattern of CA1 Pyramidal

More information

The Journal of Physiology Neuroscience

The Journal of Physiology Neuroscience J Physiol 592.4 (2014) pp 669 693 669 The Journal of Physiology Neuroscience Complementary functions of SK and Kv7/M potassium channels in excitability control and synaptic integration in rat hippocampal

More information

Light-evoked hyperpolarization and silencing of neurons by conjugated polymers

Light-evoked hyperpolarization and silencing of neurons by conjugated polymers Light-evoked hyperpolarization and silencing of neurons by conjugated polymers Paul Feyen 1,, Elisabetta Colombo 1,2,, Duco Endeman 1, Mattia Nova 1, Lucia Laudato 2, Nicola Martino 2,3, Maria Rosa Antognazza

More information

Electrophysiology. General Neurophysiology. Action Potentials

Electrophysiology. General Neurophysiology. Action Potentials 5 Electrophysiology Cochlear implants should aim to reproduce the coding of sound in the auditory system as closely as possible, for best sound perception. The cochlear implant is in part the result of

More information

Neuroscience 201A (2016) - Problems in Synaptic Physiology

Neuroscience 201A (2016) - Problems in Synaptic Physiology Question 1: The record below in A shows an EPSC recorded from a cerebellar granule cell following stimulation (at the gap in the record) of a mossy fiber input. These responses are, then, evoked by stimulation.

More information

Supplementary figure 1: LII/III GIN-cells show morphological characteristics of MC

Supplementary figure 1: LII/III GIN-cells show morphological characteristics of MC 1 2 1 3 Supplementary figure 1: LII/III GIN-cells show morphological characteristics of MC 4 5 6 7 (a) Reconstructions of LII/III GIN-cells with somato-dendritic compartments in orange and axonal arborizations

More information

The action potential travels down both branches because each branch is a typical axon with voltage dependent Na + and K+ channels.

The action potential travels down both branches because each branch is a typical axon with voltage dependent Na + and K+ channels. BIO 360 - MIDTERM FALL 2018 This is an open book, open notes exam. PLEASE WRITE YOUR NAME ON EACH SHEET. Read each question carefully and answer as well as you can. Point values are shown at the beginning

More information

Differential Effects of Short- and Long-Term Potentiation on Cell Firing in the CA1 Region of the Hippocampus

Differential Effects of Short- and Long-Term Potentiation on Cell Firing in the CA1 Region of the Hippocampus 112 The Journal of Neuroscience, January 1, 2003 23(1):112 121 Differential Effects of Short- and Long-Term Potentiation on Cell Firing in the CA1 Region of the Hippocampus Carrie P. Marder and Dean V.

More information

Supplementary Figure 1. ACE robotic platform. A. Overview of the rig setup showing major hardware components of ACE (Automatic single Cell

Supplementary Figure 1. ACE robotic platform. A. Overview of the rig setup showing major hardware components of ACE (Automatic single Cell 2 Supplementary Figure 1. ACE robotic platform. A. Overview of the rig setup showing major hardware components of ACE (Automatic single Cell Experimenter) including the MultiClamp 700B, Digidata 1440A,

More information

Factors determining the efficacy of distal excitatory synapses in rat hippocampal CA1 pyramidal neurones

Factors determining the efficacy of distal excitatory synapses in rat hippocampal CA1 pyramidal neurones Keywords: Pyramidal neurone, Dendrite, Synaptic transmission 7254 Journal of Physiology (1998), 507.2, pp. 441 462 441 Factors determining the efficacy of distal excitatory synapses in rat hippocampal

More information

Synaptic plasticityhippocampus. Neur 8790 Topics in Neuroscience: Neuroplasticity. Outline. Synaptic plasticity hypothesis

Synaptic plasticityhippocampus. Neur 8790 Topics in Neuroscience: Neuroplasticity. Outline. Synaptic plasticity hypothesis Synaptic plasticityhippocampus Neur 8790 Topics in Neuroscience: Neuroplasticity Outline Synaptic plasticity hypothesis Long term potentiation in the hippocampus How it s measured What it looks like Mechanisms

More information

Supporting Online Material for

Supporting Online Material for www.sciencemag.org/cgi/content/full/312/5779/1533/dc1 Supporting Online Material for Long-Term Potentiation of Neuron-Glia Synapses Mediated by Ca 2+ - Permeable AMPA Receptors Woo-Ping Ge, Xiu-Juan Yang,

More information

Action potential initiation and propagation in rat neocortical pyramidal neurons

Action potential initiation and propagation in rat neocortical pyramidal neurons Keywords: Action potential, Cerebral cortex, Dendrite 6798 Journal of Physiology (1997), 505.3, pp. 617 632 617 Action potential initiation and propagation in rat neocortical pyramidal neurons Greg Stuart

More information

Calcium Store Depletion Induces Persistent Perisomatic Increases in the Functional Density of h Channels in Hippocampal Pyramidal Neurons

Calcium Store Depletion Induces Persistent Perisomatic Increases in the Functional Density of h Channels in Hippocampal Pyramidal Neurons Article Calcium Store Depletion Induces Persistent Perisomatic Increases in the Functional Density of h Channels in Hippocampal Pyramidal Neurons Rishikesh Narayanan, 1,2 Kevin J. Dougherty, 1 and Daniel

More information

Intro. Comp. NeuroSci. Ch. 9 October 4, The threshold and channel memory

Intro. Comp. NeuroSci. Ch. 9 October 4, The threshold and channel memory 9.7.4 The threshold and channel memory The action potential has a threshold. In figure the area around threshold is expanded (rectangle). A current injection that does not reach the threshold does not

More information

Supporting Information

Supporting Information ATP from synaptic terminals and astrocytes regulates NMDA receptors and synaptic plasticity through PSD- 95 multi- protein complex U.Lalo, O.Palygin, A.Verkhratsky, S.G.N. Grant and Y. Pankratov Supporting

More information

Bidirectional NMDA receptor plasticity controls CA3 output and heterosynaptic metaplasticity

Bidirectional NMDA receptor plasticity controls CA3 output and heterosynaptic metaplasticity Bidirectional NMDA receptor plasticity controls CA output and heterosynaptic metaplasticity David L. Hunt, Nagore Puente, Pedro Grandes, Pablo E. Castillo a NMDAR EPSC (pa) - - -8-6 -4 - st 5 nd 5 b NMDAR

More information

Nature Neuroscience: doi: /nn Supplementary Figure 1

Nature Neuroscience: doi: /nn Supplementary Figure 1 Supplementary Figure 1 Drd1a-Cre driven ChR2 expression in the SCN. (a) Low-magnification image of a representative Drd1a-ChR2 coronal brain section (n = 2) showing endogenous tdtomato fluorescence (magenta).

More information

Increased serotonin transporter expression reduces fear and recruitment of. parvalbumin interneurons of the amygdala

Increased serotonin transporter expression reduces fear and recruitment of. parvalbumin interneurons of the amygdala Increased serotonin transporter expression reduces fear and recruitment of parvalbumin interneurons of the amygdala Marco Bocchio, Giulia Fucsina, Lydia Oikonomidis, Stephen B McHugh, David M Bannerman,

More information

Nerve. (2) Duration of the stimulus A certain period can give response. The Strength - Duration Curve

Nerve. (2) Duration of the stimulus A certain period can give response. The Strength - Duration Curve Nerve Neuron (nerve cell) is the structural unit of nervous system. Nerve is formed of large numbers of nerve fibers. Types of nerve fibers Myelinated nerve fibers Covered by myelin sheath interrupted

More information

Prolonged Synaptic Integration in Perirhinal Cortical Neurons

Prolonged Synaptic Integration in Perirhinal Cortical Neurons RAPID COMMUNICATION Prolonged Synaptic Integration in Perirhinal Cortical Neurons JOHN M. BEGGS, 1 JAMES R. MOYER, JR., 1 JOHN P. MCGANN, 2 AND THOMAS H. BROWN 1 3 1 Department of Psychology, 2 Interdepartmental

More information

KCNQ/M Channels Control Spike Afterdepolarization and Burst Generation in Hippocampal Neurons

KCNQ/M Channels Control Spike Afterdepolarization and Burst Generation in Hippocampal Neurons 4614 The Journal of Neuroscience, May 12, 2004 24(19):4614 4624 Cellular/Molecular KCNQ/M Channels Control Spike Afterdepolarization and Burst Generation in Hippocampal Neurons Cuiyong Yue and Yoel Yaari

More information

Relation across the Receptor.afferent in a Tonic Electroreceptor of Marine Catfish

Relation across the Receptor.afferent in a Tonic Electroreceptor of Marine Catfish No. 6] Proc. Japan Acad., 51 (1975) 485 102. Input. output Synapses Relation across the Receptor.afferent in a Tonic Electroreceptor of Marine Catfish By Shun-ichi UMEKITA, Yoshiko SUGAWARA, and Shosaku

More information

Presynaptic mechanisms underlying cannabinoid inhibition of excitatory synaptic transmission in rat striatal neurons

Presynaptic mechanisms underlying cannabinoid inhibition of excitatory synaptic transmission in rat striatal neurons 11488 Journal of Physiology (2001), 532.3, pp.731 748 731 Presynaptic mechanisms underlying cannabinoid inhibition of excitatory synaptic transmission in rat striatal neurons Chiung-Chun Huang, Shiow-Win

More information

Supplementary Materials for VAMP4 directs synaptic vesicles to a pool that selectively maintains asynchronous neurotransmission

Supplementary Materials for VAMP4 directs synaptic vesicles to a pool that selectively maintains asynchronous neurotransmission Supplementary Materials for VAMP4 directs synaptic vesicles to a pool that selectively maintains asynchronous neurotransmission Jesica Raingo, Mikhail Khvotchev, Pei Liu, Frederic Darios, Ying C. Li, Denise

More information

Ivy/Neurogliaform Interneurons Coordinate Activity in the Neurogenic Niche

Ivy/Neurogliaform Interneurons Coordinate Activity in the Neurogenic Niche Ivy/Neurogliaform Interneurons Coordinate Activity in the Neurogenic Niche Sean J. Markwardt, Cristina V. Dieni, Jacques I. Wadiche & Linda Overstreet-Wadiche Supplementary Methods. Animals We used hemizygous

More information

The control of spiking by synaptic input in striatal and pallidal neurons

The control of spiking by synaptic input in striatal and pallidal neurons The control of spiking by synaptic input in striatal and pallidal neurons Dieter Jaeger Department of Biology, Emory University, Atlanta, GA 30322 Key words: Abstract: rat, slice, whole cell, dynamic current

More information

Supplementary Figure 1. SDS-FRL localization of CB 1 in the distal CA3 area of the rat hippocampus. (a-d) Axon terminals (t) in stratum pyramidale

Supplementary Figure 1. SDS-FRL localization of CB 1 in the distal CA3 area of the rat hippocampus. (a-d) Axon terminals (t) in stratum pyramidale Supplementary Figure 1. SDS-FRL localization of CB 1 in the distal CA3 area of the rat hippocampus. (a-d) Axon terminals (t) in stratum pyramidale (b) show stronger immunolabeling for CB 1 than those in

More information

Differential Effect of TEA on Long-Term Synaptic Modification in Hippocampal CA1 and Dentate Gyrus in vitro

Differential Effect of TEA on Long-Term Synaptic Modification in Hippocampal CA1 and Dentate Gyrus in vitro Neurobiology of Learning and Memory 76, 375 387 (2001) doi:10.1006/nlme.2001.4032, available online at http://www.idealibrary.com on Differential Effect of TEA on Long-Term Synaptic Modification in Hippocampal

More information

Arnaud Ruiz, Emilie Campanac, Ricardo Scott, Dmitri A. Rusakov, Dimitri M. Kullmann

Arnaud Ruiz, Emilie Campanac, Ricardo Scott, Dmitri A. Rusakov, Dimitri M. Kullmann Presynaptic GABA A receptors enhance transmission and LTP induction at hippocampal mossy fiber synapses Arnaud Ruiz, Emilie Campanac, Ricardo Scott, Dmitri A. Rusakov, Dimitri M. Kullmann Supplementary

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nature10776 Supplementary Information 1: Influence of inhibition among blns on STDP of KC-bLN synapses (simulations and schematics). Unconstrained STDP drives network activity to saturation

More information

Vizcarra-Chacón et al. BMC Neuroscience 2013, 14:60

Vizcarra-Chacón et al. BMC Neuroscience 2013, 14:60 Vizcarra-Chacón et al. BMC Neuroscience 2013, 14:60 RESEARCH ARTICLE Open Access Contribution of different classes of glutamate receptors in the corticostriatal polysynaptic responses from striatal direct

More information

Nature Neuroscience: doi: /nn.2275

Nature Neuroscience: doi: /nn.2275 Supplementary Figure S1. The presence of MeCP2 in enriched primary glial cultures from rat or mouse brains is not neuronal. Western blot analysis of protein extracts from (a) rat glial and neuronal cultures.

More information

Epileptic pilocarpine-treated rats exhibit aberrant hippocampal EPSP-spike potentiation but retain long-term potentiation

Epileptic pilocarpine-treated rats exhibit aberrant hippocampal EPSP-spike potentiation but retain long-term potentiation ORIGINAL RESEARCH Physiological Reports ISSN 25-87X Epileptic pilocarpine-treated rats exhibit aberrant hippocampal EPSP-spike potentiation but retain long-term potentiation Ezekiel Carpenter-Hyland*,

More information

Supplemental information Acid-sensing ion channel 1a contributes to hippocampal LTP inducibility through multiple mechanisms

Supplemental information Acid-sensing ion channel 1a contributes to hippocampal LTP inducibility through multiple mechanisms Supplemental information Acid-sensing ion channel 1a contributes to hippocampal LTP inducibility through multiple mechanisms Ming-Gang Liu, Hu-Song Li, Wei-Guang Li, Yan-Jiao Wu, Shi-Ning Deng, Chen Huang,

More information

Neurons of the Bed Nucleus of the Stria Terminalis (BNST)

Neurons of the Bed Nucleus of the Stria Terminalis (BNST) Neurons of the Bed Nucleus of the Stria Terminalis (BNST) Electrophysiological Properties and Their Response to Serotonin DONALD G. RAINNIE a Harvard Medical School and Department of Psychiatry, Brockton

More information

Supplementary Table I Blood pressure and heart rate measurements pre- and post-stroke

Supplementary Table I Blood pressure and heart rate measurements pre- and post-stroke SUPPLEMENTARY INFORMATION doi:10.1038/nature09511 Supplementary Table I Blood pressure and heart rate measurements pre- and post-stroke Pre Post 7-days Systolic Diastolic BPM Systolic Diastolic BPM Systolic

More information

Modulation of Inhibitory Synaptic Potentials in the Piriform Cortex

Modulation of Inhibitory Synaptic Potentials in the Piriform Cortex Modulation of Inhibitory Synaptic Potentials in the Piriform Cortex MADHVI M. PATIL AND MICHAEL E. HASSELMO Department of Psychology, Boston University, Boston 02215; and Department of Psychology and Program

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Localization of virus injections. (a) Schematic showing the approximate center of AAV-DIO-ChR2-YFP injection sites in the NAc of Dyn-cre mice (n=8 mice, 16 injections; caudate/putamen,

More information

Unique functional properties of somatostatin-expressing GABAergic neurons in mouse barrel cortex

Unique functional properties of somatostatin-expressing GABAergic neurons in mouse barrel cortex Supplementary Information Unique functional properties of somatostatin-expressing GABAergic neurons in mouse barrel cortex Luc Gentet, Yves Kremer, Hiroki Taniguchi, Josh Huang, Jochen Staiger and Carl

More information

Synaptic Transmission: Ionic and Metabotropic

Synaptic Transmission: Ionic and Metabotropic Synaptic Transmission: Ionic and Metabotropic D. Purves et al. Neuroscience (Sinauer Assoc.) Chapters 5, 6, 7. C. Koch. Biophysics of Computation (Oxford) Chapter 4. J.G. Nicholls et al. From Neuron to

More information

Nature Neuroscience: doi: /nn Supplementary Figure 1

Nature Neuroscience: doi: /nn Supplementary Figure 1 Supplementary Figure 1 Relative expression of K IR2.1 transcript to enos was reduced 29-fold in capillaries from knockout animals. Relative expression of K IR2.1 transcript to enos was reduced 29-fold

More information

EPSP was necessary. Because action potentials were only elicited by a subset of

EPSP was necessary. Because action potentials were only elicited by a subset of Journal of Physiology (1992), 453, pp. 663-687 663 With 11 figures Printed in Great Britain SYNAPTICALLY TRIGGRD ACTION POTNTIALS BGIN AS A DPOLARIZING RAMP IN RAT HIPPOCAMPAL NURONS IN VITRO BY G.-Y.

More information

The mammalian cochlea possesses two classes of afferent neurons and two classes of efferent neurons.

The mammalian cochlea possesses two classes of afferent neurons and two classes of efferent neurons. 1 2 The mammalian cochlea possesses two classes of afferent neurons and two classes of efferent neurons. Type I afferents contact single inner hair cells to provide acoustic analysis as we know it. Type

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION doi:10.1038/nature11306 Supplementary Figures Supplementary Figure 1. Basic characterization of GFP+ RGLs in the dentate gyrus of adult nestin-gfp mice. a, Sample confocal images

More information

Induction of Hebbian and Non-Hebbian Mossy Fiber Long-Term Potentiation by Distinct Patterns of High-Frequency Stimulation

Induction of Hebbian and Non-Hebbian Mossy Fiber Long-Term Potentiation by Distinct Patterns of High-Frequency Stimulation The Journal of Neuroscience, July 1, 1996, 16(13):4293 4299 Induction of Hebbian and Non-Hebbian Mossy Fiber Long-Term Potentiation by Distinct Patterns of High-Frequency Stimulation Nathaniel N. Urban

More information

STRUCTURAL ELEMENTS OF THE NERVOUS SYSTEM

STRUCTURAL ELEMENTS OF THE NERVOUS SYSTEM STRUCTURAL ELEMENTS OF THE NERVOUS SYSTEM STRUCTURE AND MAINTENANCE OF NEURONS (a) (b) Dendrites Cell body Initial segment collateral terminals (a) Diagrammatic representation of a neuron. The break in

More information

Physiology of the nerve

Physiology of the nerve Physiology of the nerve Objectives Transmembrane potential Action potential Relative and absolute refractory period The all-or-none law Hoorweg Weiss curve Du Bois Reymond principle Types of nerve fibres

More information

A Role for Synaptic Inputs at Distal Dendrites: Instructive Signals for Hippocampal Long-Term Plasticity

A Role for Synaptic Inputs at Distal Dendrites: Instructive Signals for Hippocampal Long-Term Plasticity Article A Role for Synaptic Inputs at Distal Dendrites: Instructive Signals for Hippocampal Long-Term Plasticity Joshua T. Dudman, 1 David Tsay, 1 and Steven A. Siegelbaum 1,2,3, * 1 Department of Neuroscience

More information

Introduction to Neurobiology

Introduction to Neurobiology Biology 240 General Zoology Introduction to Neurobiology Nervous System functions: communication of information via nerve signals integration and processing of information control of physiological and

More information

Cholinergic Activation of M2 Receptors Leads to Context- Dependent Modulation of Feedforward Inhibition in the Visual Thalamus

Cholinergic Activation of M2 Receptors Leads to Context- Dependent Modulation of Feedforward Inhibition in the Visual Thalamus Cholinergic Activation of M2 Receptors Leads to Context- Dependent Modulation of Feedforward Inhibition in the Visual Thalamus Miklos Antal., Claudio Acuna-Goycolea., R. Todd Pressler, Dawn M. Blitz, Wade

More information

Synaptic and Network Mechanisms of Sparse and Reliable Visual Cortical Activity during Nonclassical Receptive Field Stimulation

Synaptic and Network Mechanisms of Sparse and Reliable Visual Cortical Activity during Nonclassical Receptive Field Stimulation Article Synaptic and Network Mechanisms of Sparse and Reliable Visual Cortical Activity during Nonclassical Receptive Field Stimulation Bilal Haider, 1 Matthew R. Krause, 1 Alvaro Duque, 1 Yuguo Yu, 1

More information

Nature Methods: doi: /nmeth Supplementary Figure 1. Activity in turtle dorsal cortex is sparse.

Nature Methods: doi: /nmeth Supplementary Figure 1. Activity in turtle dorsal cortex is sparse. Supplementary Figure 1 Activity in turtle dorsal cortex is sparse. a. Probability distribution of firing rates across the population (notice log scale) in our data. The range of firing rates is wide but

More information

-51mV 30s 3mV. n=14 n=4 p=0.4. Depolarization (mv) 3

-51mV 30s 3mV. n=14 n=4 p=0.4. Depolarization (mv) 3 Supplementary Figure 1 a optoβ 2 -AR b ChR2-51mV 30s 3mV -50mV 30s 3mV c 4 n=14 n=4 p=0.4 Depolarization (mv) 3 2 1 0 Both optogenetic actuators, optoβ 2 AR and ChR2, were effective in stimulating astrocytes

More information

CELLULAR NEUROPHYSIOLOGY

CELLULAR NEUROPHYSIOLOGY CELLULAR NEUROPHYSIOLOGY CONSTANCE HAMMOND 4. SYNAPTIC TRANSMISSION II: GLUTAMATERGIC TRANSMISSION Video 4-1: Observations and glutamate receptor channels Synaptic transmission II 1 Constance Hammond Observation

More information

Postsynaptic Complex Spike Bursting Enables the Induction of LTP by Theta Frequency Synaptic Stimulation

Postsynaptic Complex Spike Bursting Enables the Induction of LTP by Theta Frequency Synaptic Stimulation The Journal of Neuroscience, September 15, 1998, 18(18):7118 7126 Postsynaptic Complex Spike Bursting Enables the Induction of LTP by Theta Frequency Synaptic Stimulation Mark J. Thomas, 1 Ayako M. Watabe,

More information

Linking Neuronal Ensembles by Associative Synaptic Plasticity

Linking Neuronal Ensembles by Associative Synaptic Plasticity Linking Neuronal Ensembles by Associative Synaptic Plasticity Qi Yuan 1,2, Jeffry S. Isaacson 2, Massimo Scanziani 1,2,3 * 1 Department of Neurobiology, Center for Neural Circuits and Behavior, University

More information

Bursting dynamics in the brain. Jaeseung Jeong, Department of Biosystems, KAIST

Bursting dynamics in the brain. Jaeseung Jeong, Department of Biosystems, KAIST Bursting dynamics in the brain Jaeseung Jeong, Department of Biosystems, KAIST Tonic and phasic activity A neuron is said to exhibit a tonic activity when it fires a series of single action potentials

More information

Supplementary Figure 1: Kv7 currents in neonatal CA1 neurons measured with the classic M- current voltage-clamp protocol.

Supplementary Figure 1: Kv7 currents in neonatal CA1 neurons measured with the classic M- current voltage-clamp protocol. Supplementary Figures 1-11 Supplementary Figure 1: Kv7 currents in neonatal CA1 neurons measured with the classic M- current voltage-clamp protocol. (a), Voltage-clamp recordings from CA1 pyramidal neurons

More information

POSTSYNAPTIC INHIBITION OF CRAYFISH TONIC FLEXOR MOTOR NEURONES BY ESCAPE COMMANDS

POSTSYNAPTIC INHIBITION OF CRAYFISH TONIC FLEXOR MOTOR NEURONES BY ESCAPE COMMANDS J. exp. Biol. (1980), 85, 343-347 343 With a figures Printed in Great Britain POSTSYNAPTIC INHIBITION OF CRAYFISH TONIC FLEXOR MOTOR NEURONES BY ESCAPE COMMANDS BY J. Y. KUWADA, G. HAGIWARA AND J. J. WINE

More information

Part 11: Mechanisms of Learning

Part 11: Mechanisms of Learning Neurophysiology and Information: Theory of Brain Function Christopher Fiorillo BiS 527, Spring 2012 042 350 4326, fiorillo@kaist.ac.kr Part 11: Mechanisms of Learning Reading: Bear, Connors, and Paradiso,

More information

Supplementary Information. Errors in the measurement of voltage activated ion channels. in cell attached patch clamp recordings

Supplementary Information. Errors in the measurement of voltage activated ion channels. in cell attached patch clamp recordings Supplementary Information Errors in the measurement of voltage activated ion channels in cell attached patch clamp recordings Stephen R. Williams 1,2 and Christian Wozny 2 1 Queensland Brain Institute,

More information

Visualization of the spread of electrical activity in rat hippocampal slices by voltage-sensitive optical probes. A. Grinvald, A. Manker and M.

Visualization of the spread of electrical activity in rat hippocampal slices by voltage-sensitive optical probes. A. Grinvald, A. Manker and M. Visualization of the spread of electrical activity in rat hippocampal slices by voltage-sensitive optical probes A. Grinvald, A. Manker and M. Segal J. Physiol. 1982;333;269-291 This information is current

More information

Direct current stimulation modulates LTP and LTD: activity-dependence and dendritic effects

Direct current stimulation modulates LTP and LTD: activity-dependence and dendritic effects City University of New York (CUNY) CUNY Academic Works Master's Theses City College of New York 2016 Direct current stimulation modulates LTP and LTD: activity-dependence and dendritic effects Gregory

More information

TRPA1 channels regulate astrocyte resting calcium. and inhibitory synapse efficacy through GAT-3

TRPA1 channels regulate astrocyte resting calcium. and inhibitory synapse efficacy through GAT-3 TRPA1 channels regulate astrocyte resting calcium and inhibitory synapse efficacy through GAT-3 * 1 Eiji Shigetomi, * 1 Xiaoping Tong 3 Kelvin Y. Kwan, 3 David P. Corey & 1,2 Baljit S. Khakh Ψ 1 Departments

More information

Neuroscience 201A Problem Set #1, 27 September 2016

Neuroscience 201A Problem Set #1, 27 September 2016 Neuroscience 201A Problem Set #1, 27 September 2016 1. The figure above was obtained from a paper on calcium channels expressed by dentate granule cells. The whole-cell Ca 2+ currents in (A) were measured

More information

Dep. Control Time (min)

Dep. Control Time (min) aa Control Dep. RP 1s 1 mv 2s 1 mv b % potentiation of IPSP 2 15 1 5 Dep. * 1 2 3 4 Time (min) Supplementary Figure 1. Rebound potentiation of IPSPs in PCs. a, IPSPs recorded with a K + gluconate pipette

More information

Fig. S4. Current-voltage relations of iglurs. A-C: time courses of currents evoked by 100 ms pulses

Fig. S4. Current-voltage relations of iglurs. A-C: time courses of currents evoked by 100 ms pulses Fig. S1. Immunohistochemical detection of iglur2 protein in single islet cells. A: α cells identified using glucagon-specific antibody express the iglur2 subtype of AMPA receptor. 24 out of 26 identified

More information

Brief anoxic episodes induce long-lasting changes in synaptic properties of rat CA3 hippocampal neurons

Brief anoxic episodes induce long-lasting changes in synaptic properties of rat CA3 hippocampal neurons Neuroscience Letters, 90 (1988) 273-278 273 Elsevier Scientific Publishers Ireland Ltd. NSL 05456 Brief anoxic episodes induce long-lasting changes in synaptic properties of rat CA3 hippocampal neurons

More information

BIPN 140 Problem Set 6

BIPN 140 Problem Set 6 BIPN 140 Problem Set 6 1) The hippocampus is a cortical structure in the medial portion of the temporal lobe (medial temporal lobe in primates. a) What is the main function of the hippocampus? The hippocampus

More information

1) Drop off in the Bi 150 box outside Baxter 331 or to the head TA (jcolas).

1) Drop off in the Bi 150 box outside Baxter 331 or  to the head TA (jcolas). Bi/CNS/NB 150 Problem Set 3 Due: Tuesday, Oct. 27, at 4:30 pm Instructions: 1) Drop off in the Bi 150 box outside Baxter 331 or e-mail to the head TA (jcolas). 2) Submit with this cover page. 3) Use a

More information

BIPN 140 Problem Set 6

BIPN 140 Problem Set 6 BIPN 140 Problem Set 6 1) Hippocampus is a cortical structure in the medial portion of the temporal lobe (medial temporal lobe in primates. a) What is the main function of the hippocampus? The hippocampus

More information

Supplementary Figure S1: Histological analysis of kainate-treated animals

Supplementary Figure S1: Histological analysis of kainate-treated animals Supplementary Figure S1: Histological analysis of kainate-treated animals Nissl stained coronal or horizontal sections were made from kainate injected (right) and saline injected (left) animals at different

More information

Marianna Szemes 1, Rachel L Davies 2, Claire LP Garden 3 and Maria M Usowicz 4*

Marianna Szemes 1, Rachel L Davies 2, Claire LP Garden 3 and Maria M Usowicz 4* Szemes et al. Molecular Brain 2013, 6:33 RESEARCH Open Access Weaker control of the electrical properties of cerebellar granule cells by tonically active GABA A receptors in the Ts65Dn mouse model of Down

More information

The Journal of Physiology Neuroscience

The Journal of Physiology Neuroscience J Physiol 591.22 (2013) pp 5765 5790 5765 The Journal of Physiology Neuroscience Long-lasting hyperpolarization underlies seizure reduction by low frequency deep brain electrical stimulation Sheela Toprani

More information

BIONB/BME/ECE 4910 Neuronal Simulation Assignments 1, Spring 2013

BIONB/BME/ECE 4910 Neuronal Simulation Assignments 1, Spring 2013 BIONB/BME/ECE 4910 Neuronal Simulation Assignments 1, Spring 2013 Tutorial Assignment Page Due Date Week 1/Assignment 1: Introduction to NIA 1 January 28 The Membrane Tutorial 9 Week 2/Assignment 2: Passive

More information

Selective Shunting of the NMDA EPSP Component by the Slow Afterhyperpolarization in Rat CA1 Pyramidal Neurons

Selective Shunting of the NMDA EPSP Component by the Slow Afterhyperpolarization in Rat CA1 Pyramidal Neurons J Neurophysiol 97: 3242 3255, 2007. First published February 28, 2007; doi:10.1152/jn.00422.2006. Selective Shunting of the NMDA EPSP Component by the Slow Afterhyperpolarization in Rat CA1 Pyramidal Neurons

More information

Neuromodulation of hippocampal single cell- and network activity by human cerebrospinal fluid

Neuromodulation of hippocampal single cell- and network activity by human cerebrospinal fluid Neuromodulation of hippocampal single cell- and network activity by human cerebrospinal fluid Andreas Björefeldt 2015 Department of Physiology Institute of Neuroscience and Physiology Sahlgrenska academy

More information

Nature Neuroscience: doi: /nn Supplementary Figure 1. Trial structure for go/no-go behavior

Nature Neuroscience: doi: /nn Supplementary Figure 1. Trial structure for go/no-go behavior Supplementary Figure 1 Trial structure for go/no-go behavior a, Overall timeline of experiments. Day 1: A1 mapping, injection of AAV1-SYN-GCAMP6s, cranial window and headpost implantation. Water restriction

More information